Rorug06G0135300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002183
Physical Location & Seq
Reverse (-)
19144676 .. 19148099
3424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0135300.1

Sequence Viewer

Length: 1203 bp
ATGACGTCGACCGAGACAGAGGACGCCCTACGACGTCGCACCGCCATCACCGATTACCGGAAGAGGCTGCTCCAGCACAAGGAGCTCGACTCCCGAGTCCGCGCAGTGCGGGAAAACTTGCGAGGAGCTAAGAAGGAGTTTGGGAAAACTGAAGATGATTTGAAGTCGCTTCAAAGTGTTGGTCAGATTATAGGGGAAGTTCTGAGACCTCTCGATAATGAACGCTTGATAGTGAAGGCGAGTAGTGGCCCTAGGTATGTGGTTGGCTGTCGCAGTAAAGTGGACAAAGAAAAACTAACAGCGGGCACTCGGGTTGTTTTGGATATGACTACTCTGACTATCATGAGGGCTCTTCCAAGAGAGGTTGATCCAGTAGTGTATAATATGCTTCATGAAGATCCTGGTAATGTTAGCTACTCCGCTGTGGGAGGGCTAGGAGACCAGATCCGGGAGCTTAGGGAATCTATAGAGCTGCCTCTAATGAACCCTGAGCTCTTCCTTAGAGTGGGCATCAAACCACCCAAGGGTGTTCTTCTCTATGGACCTCCGGGAACAGGGAAGACATTGCTAGCCAGAGCAATTGCGAGCAACATAGATGCCAACTTTCTAAAGGTTGTATCAAGCGCCATTATTGATAAGTACATTGGTGAAAGTGCAAGATTGATTAGGGAAATGTTTGGTTATGCTCGTGATCACCAGCCCTGTATCATTTTTATGGACGAGATCGATGCCATCGGTGGACGGCGTTTTAGCGAAGGGACCAGTGCAGACCGTGAAATCCAGCGAACACTCATGGAGTTGCTTAACCAGTTAGATGGATTTGATCAACTTGGGAAGGTGAAAATGATAATGGCAACAAATAGACCTGATGTTTTAGATCCTGCACTTCTCCGACCTGGGAGACTAGACCGGAAAATCGAGATTCCATTGCCTAATGAACAGTCAAGAATGGAAATTCTTAAAATTCATGCTGCTGGAATAGCTAAACATGGGGAAATTGATTATGAGGCAGTTGTGAAGCTTGCTGAGGGCTTTAATGGAGCAGATCTGCGGAATGTCTGCACTGAAGCTGGGATGTCTGCGATCCGTGCTGAACGCGATTATGTCATTCATGAAGACTTCATGAAGGCTGTAAGAAAATTGAACGAAGCCAAGAAGCTGGAATCAAGTGCACACTATAATGCTGATTTTGGAAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

400

Amino Acids

44.77

Weight (kDa)

8.25

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 64 - 119 1e-14 Proteasomal ATPase OB C-terminal domain
RuvB_N PF05496 176 - 243 8.7e-06 Holliday junction DNA helicase RuvB P-loop domain
AAA PF00004 177 - 310 1.3e-43 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 333 - 375 1.5e-12 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 95
AatII GACGTC 2 cut(s) 8, 37
AccI GTMKAC 1 cut(s) 8
AccII CGCG 2 cut(s) 102, 1098
AciI CCGC 6 cut(s) 42, 100, 109, 302, 420, 1051
AclWI GGATC 5 cut(s) 362, 392, 439, 872, 1078
AcsI RAATTY 2 cut(s) 954, 963
AcuI CTGAAG 2 cut(s) 171, 1086
AcyI GRCGYC 3 cut(s) 5, 24, 34
AfaI GTAC 1 cut(s) 641
AfiI CCNNNNNNNGG 6 cut(s) 57, 79, 448, 505, 524, 554
AgsI TTSAA 3 cut(s) 163, 173, 1144
AjnI CCWGG 2 cut(s) 400, 895
Alw21I GWGCWC 3 cut(s) 87, 495, 1174
Alw26I GTCTC 4 cut(s) 8, 199, 432, 895
Alw44I GTGCAC 1 cut(s) 1170
AlwI GGATC 5 cut(s) 362, 392, 439, 872, 1078
Ama87I CYCGRG 2 cut(s) 93, 309
AoxI GGCC 1 cut(s) 247
ApaLI GTGCAC 1 cut(s) 1170
ApeKI GCWGC 3 cut(s) 67, 472, 971
ApoI RAATTY 2 cut(s) 954, 963
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
AspA2I CCTAGG 1 cut(s) 251
AspLEI GCGC 2 cut(s) 104, 626
AspS9I GGNCC 3 cut(s) 248, 542, 759
AsuC2I CCSGG 2 cut(s) 449, 549
AsuHPI GGTGA 4 cut(s) 40, 659, 686, 850
AsuNHI GCTAGC 1 cut(s) 568
AvaI CYCGRG 2 cut(s) 93, 309
AvaII GGWCC 2 cut(s) 542, 759
AvrII CCTAGG 1 cut(s) 251
BaeGI GKGCMC 2 cut(s) 308, 1174
BanII GRGCYC 3 cut(s) 87, 352, 495
BauI CACGAG 1 cut(s) 687
BbsI GAAGAC 2 cut(s) 566, 1122
Bbv12I GWGCWC 3 cut(s) 87, 495, 1174
BbvCI CCTCAGC 1 cut(s) 1026
BbvI GCAGC 3 cut(s) 54, 459, 958
BccI CCATC 3 cut(s) 53, 740, 809
BceAI ACGGC 1 cut(s) 758
BcgI CGANNNNNNTGC 2 cut(s) 710, 744
BciT130I CCWGG 2 cut(s) 402, 897
BclI TGATCA 2 cut(s) 691, 823
BcnI CCSGG 2 cut(s) 449, 549
BcoDI GTCTC 4 cut(s) 8, 199, 432, 895
BfaI CTAG 4 cut(s) 252, 434, 569, 905
BfmI CTRYAG 1 cut(s) 465
BfoI RGCGCY 1 cut(s) 627
BglII AGATCT 1 cut(s) 1045
BisI GCNGC 3 cut(s) 68, 473, 972
BlnI CCTAGG 1 cut(s) 251
BlsI GCNGC 3 cut(s) 69, 474, 973
Bme1390I CCNGG 4 cut(s) 402, 449, 549, 897
Bme18I GGWCC 2 cut(s) 542, 759
BmeT110I CYCGRG 2 cut(s) 93, 309
BmgT120I GGNCC 3 cut(s) 248, 542, 759
BmiI GGNNCC 1 cut(s) 760
BmrFI CCNGG 4 cut(s) 402, 449, 549, 897
BmsI GCATC 3 cut(s) 519, 586, 718
BmtI GCTAGC 1 cut(s) 572
BpiI GAAGAC 2 cut(s) 566, 1122
BplI GAGNNNNNCTC 2 cut(s) 74, 106
BpmI CTGGAG 1 cut(s) 56
Bpu10I CCTNAGC 3 cut(s) 455, 489, 1026
BpuMI CCSGG 2 cut(s) 449, 549
Bsa29I ATCGAT 1 cut(s) 726
BsaHI GRCGYC 3 cut(s) 5, 24, 34
BsaI GGTCTC 2 cut(s) 199, 432
BsaJI CCNNGG 3 cut(s) 251, 522, 896
BsaWI WCCGGW 2 cut(s) 57, 909
Bsc4I CCNNNNNNNGG 6 cut(s) 57, 79, 448, 505, 524, 554
Bse1I ACTGG 3 cut(s) 371, 762, 808
Bse3DI GCAATG 2 cut(s) 563, 926
BseBI CCWGG 2 cut(s) 402, 897
BseCI ATCGAT 1 cut(s) 726
BseDI CCNNGG 3 cut(s) 251, 522, 896
BseGI GGATG 1 cut(s) 1080
BseLI CCNNNNNNNGG 6 cut(s) 57, 79, 448, 505, 524, 554
BseMI GCAATG 2 cut(s) 563, 926
BseMII CTCAG 3 cut(s) 194, 480, 1017
BseNI ACTGG 3 cut(s) 371, 762, 808
BseRI GAGGAG 1 cut(s) 138
BseSI GKGCMC 2 cut(s) 308, 1174
BseXI GCAGC 3 cut(s) 54, 459, 958
BseYI CCCAGC 1 cut(s) 1070
BsgI GTGCAG 3 cut(s) 786, 867, 1045
Bsh1236I CGCG 2 cut(s) 102, 1098
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 1 cut(s) 249
BshVI ATCGAT 1 cut(s) 726
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 3 cut(s) 87, 495, 1174
BsiHKCI CYCGRG 2 cut(s) 93, 309
BsiSI CCGG 4 cut(s) 58, 448, 548, 910
BslFI GGGAC 1 cut(s) 772
BslI CCNNNNNNNGG 6 cut(s) 57, 79, 448, 505, 524, 554
BsmAI GTCTC 4 cut(s) 8, 199, 432, 895
BsmFI GGGAC 1 cut(s) 772
BsnI GGCC 1 cut(s) 249
Bso31I GGTCTC 2 cut(s) 199, 432
BsoBI CYCGRG 2 cut(s) 93, 309
Bsp1286I GDGCHC 5 cut(s) 87, 308, 352, 495, 1174
Bsp143I GATC 9 cut(s) 367, 397, 444, 691, 723, 823, 877, 1045, 1083
BspACI CCGC 6 cut(s) 42, 100, 109, 302, 420, 1051
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 3 cut(s) 195, 481, 1018
BspDI ATCGAT 1 cut(s) 726
BspFNI CGCG 2 cut(s) 102, 1098
BspHI TCATGA 4 cut(s) 342, 391, 1111, 1122
BspLI GGNNCC 1 cut(s) 760
BspOI GCTAGC 1 cut(s) 572
BspPI GGATC 5 cut(s) 362, 392, 439, 872, 1078
BspQI GCTCTTC 2 cut(s) 357, 500
BspTNI GGTCTC 2 cut(s) 199, 432
BsrDI GCAATG 2 cut(s) 563, 926
BsrI ACTGG 3 cut(s) 371, 762, 808
BssECI CCNNGG 3 cut(s) 251, 522, 896
BssMI GATC 9 cut(s) 367, 397, 444, 691, 723, 823, 877, 1045, 1083
BssNI GRCGYC 3 cut(s) 5, 24, 34
BssSI CACGAG 1 cut(s) 687
BssT1I CCWWGG 2 cut(s) 251, 522
Bst2BI CACGAG 1 cut(s) 687
Bst2UI CCWGG 2 cut(s) 402, 897
Bst4CI ACNGT 2 cut(s) 773, 942
Bst6I CTCTTC 3 cut(s) 56, 357, 500
BstACI GRCGYC 3 cut(s) 5, 24, 34
BstC8I GCNNGC 4 cut(s) 304, 570, 586, 1023
BstDEI CTNAG 6 cut(s) 129, 203, 455, 489, 500, 1026
BstF5I GGATG 1 cut(s) 1080
BstFNI CGCG 2 cut(s) 102, 1098
BstH2I RGCGCY 1 cut(s) 627
BstHHI GCGC 2 cut(s) 104, 626
BstKTI GATC 9 cut(s) 370, 400, 447, 694, 726, 826, 880, 1048, 1086
BstMAI GTCTC 4 cut(s) 8, 199, 432, 895
BstMBI GATC 9 cut(s) 367, 397, 444, 691, 723, 823, 877, 1045, 1083
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 4 cut(s) 73, 82, 980, 1088
BstNI CCWGG 2 cut(s) 402, 897
BstSCI CCNGG 4 cut(s) 400, 447, 547, 895
BstSFI CTRYAG 1 cut(s) 465
BstSLI GKGCMC 2 cut(s) 308, 1174
BstUI CGCG 2 cut(s) 102, 1098
BstV1I GCAGC 3 cut(s) 54, 459, 958
BstV2I GAAGAC 2 cut(s) 566, 1122
BstX2I RGATCY 4 cut(s) 397, 444, 877, 1045
BstXI CCANNNNNNTGG 2 cut(s) 815, 1159
BstYI RGATCY 4 cut(s) 397, 444, 877, 1045
Bsu15I ATCGAT 1 cut(s) 726
BsuRI GGCC 1 cut(s) 249
BsuTUI ATCGAT 1 cut(s) 726
BtsCI GGATG 1 cut(s) 1080
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 3 cut(s) 111, 769, 1062
Cac8I GCNNGC 4 cut(s) 304, 570, 586, 1023
CciI TCATGA 4 cut(s) 342, 391, 1111, 1122
CfoI GCGC 2 cut(s) 104, 626
Cfr13I GGNCC 3 cut(s) 248, 542, 759
ClaI ATCGAT 1 cut(s) 726
CseI GACGC 1 cut(s) 32
Csp6I GTAC 1 cut(s) 640
CviAII CATG 7 cut(s) 343, 392, 793, 968, 989, 1112, 1123
CviQI GTAC 1 cut(s) 640
DdeI CTNAG 6 cut(s) 129, 203, 455, 489, 500, 1026
DpnI GATC 9 cut(s) 369, 399, 446, 693, 725, 825, 879, 1047, 1085
DpnII GATC 9 cut(s) 367, 397, 444, 691, 723, 823, 877, 1045, 1083
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
Eam1104I CTCTTC 3 cut(s) 56, 357, 500
EarI CTCTTC 3 cut(s) 56, 357, 500
Ecl136II GAGCTC 2 cut(s) 85, 493
Eco130I CCWWGG 2 cut(s) 251, 522
Eco24I GRGCYC 3 cut(s) 87, 352, 495
Eco31I GGTCTC 2 cut(s) 199, 432
Eco47I GGWCC 2 cut(s) 542, 759
Eco53kI GAGCTC 2 cut(s) 85, 493
Eco57I CTGAAG 2 cut(s) 171, 1086
Eco88I CYCGRG 2 cut(s) 93, 309
EcoICRI GAGCTC 2 cut(s) 85, 493
EcoRII CCWGG 2 cut(s) 400, 895
EcoT14I CCWWGG 2 cut(s) 251, 522
EcoT38I GRGCYC 3 cut(s) 87, 352, 495
ErhI CCWWGG 2 cut(s) 251, 522
FaeI CATG 7 cut(s) 346, 395, 796, 971, 992, 1115, 1126
FaqI GGGAC 1 cut(s) 772
FatI CATG 7 cut(s) 342, 391, 792, 967, 988, 1111, 1122
FauI CCCGC 2 cut(s) 102, 295
FbaI TGATCA 2 cut(s) 691, 823
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 3 cut(s) 68, 473, 972
FokI GGATG 1 cut(s) 1087
FriOI GRGCYC 3 cut(s) 87, 352, 495
Fsp4HI GCNGC 3 cut(s) 68, 473, 972
FspBI CTAG 4 cut(s) 252, 434, 569, 905
GlaI GCGC 2 cut(s) 103, 625
GluI GCNGC 3 cut(s) 68, 473, 972
GsaI CCCAGC 1 cut(s) 1074
GsuI CTGGAG 1 cut(s) 56
HaeII RGCGCY 1 cut(s) 627
HaeIII GGCC 1 cut(s) 249
HapII CCGG 4 cut(s) 58, 448, 548, 910
HgaI GACGC 1 cut(s) 32
HhaI GCGC 2 cut(s) 104, 626
Hin1I GRCGYC 3 cut(s) 5, 24, 34
Hin1II CATG 7 cut(s) 346, 395, 796, 971, 992, 1115, 1126
Hin6I GCGC 2 cut(s) 102, 624
HinP1I GCGC 2 cut(s) 102, 624
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HindIII AAGCTT 1 cut(s) 1019
HinfI GANTC 5 cut(s) 89, 96, 461, 922, 1163
HpaII CCGG 4 cut(s) 58, 448, 548, 910
HphI GGTGA 4 cut(s) 40, 659, 686, 850
Hpy166II GTNNAC 4 cut(s) 9, 283, 740, 1172
Hpy188I TCNGA 4 cut(s) 186, 204, 336, 893
Hpy188III TCNNGA 9 cut(s) 93, 212, 343, 392, 689, 919, 945, 1112, 1123
Hpy8I GTNNAC 4 cut(s) 9, 283, 740, 1172
Hpy99I CGWCG 3 cut(s) 10, 36, 39
HpyAV CCTTC 5 cut(s) 127, 229, 749, 829, 1120
HpyCH4III ACNGT 2 cut(s) 773, 942
HpyCH4IV ACGT 2 cut(s) 5, 34
HpyCH4V TGCA 5 cut(s) 656, 767, 884, 1062, 1172
HpyF10VI GCNNNNNNNGC 4 cut(s) 73, 82, 980, 1088
HpyF3I CTNAG 6 cut(s) 129, 203, 455, 489, 500, 1026
HpySE526I ACGT 2 cut(s) 5, 34
Hsp92I GRCGYC 3 cut(s) 5, 24, 34
Hsp92II CATG 7 cut(s) 346, 395, 796, 971, 992, 1115, 1126
HspAI GCGC 2 cut(s) 102, 624
Ksp22I TGATCA 2 cut(s) 691, 823
Kzo9I GATC 9 cut(s) 367, 397, 444, 691, 723, 823, 877, 1045, 1083
LguI GCTCTTC 2 cut(s) 357, 500
LmnI GCTCC 5 cut(s) 75, 82, 125, 451, 1040
Lsp1109I GCAGC 3 cut(s) 54, 459, 958
LweI GCATC 3 cut(s) 519, 586, 718
MaeI CTAG 4 cut(s) 252, 434, 569, 905
MaeII ACGT 2 cut(s) 5, 34
MalI GATC 9 cut(s) 369, 399, 446, 693, 725, 825, 879, 1047, 1085
MboI GATC 9 cut(s) 367, 397, 444, 691, 723, 823, 877, 1045, 1083
MboII GAAGA 8 cut(s) 73, 164, 344, 407, 487, 524, 571, 1127
MfeI CAATTG 1 cut(s) 579
MflI RGATCY 4 cut(s) 397, 444, 877, 1045
MhlI GDGCHC 5 cut(s) 87, 308, 352, 495, 1174
MluCI AATT 5 cut(s) 579, 954, 963, 996, 1139
MlyI GAGTC 2 cut(s) 83, 105
MmeI TCCRAC 1 cut(s) 916
MseI TTAA 3 cut(s) 804, 960, 1035
MslI CAYNNNNRTG 2 cut(s) 713, 1179
MspA1I CMGCKG 2 cut(s) 302, 422
MspI CCGG 4 cut(s) 58, 448, 548, 910
MspR9I CCNGG 4 cut(s) 402, 449, 549, 897
MunI CAATTG 1 cut(s) 579
MvaI CCWGG 2 cut(s) 402, 897
MvnI CGCG 2 cut(s) 102, 1098
MwoI GCNNNNNNNGC 4 cut(s) 73, 82, 980, 1088
NciI CCSGG 2 cut(s) 449, 549
NdeII GATC 9 cut(s) 367, 397, 444, 691, 723, 823, 877, 1045, 1083
NheI GCTAGC 1 cut(s) 568
NlaIII CATG 7 cut(s) 346, 395, 796, 971, 992, 1115, 1126
NlaIV GGNNCC 1 cut(s) 760
PagI TCATGA 4 cut(s) 342, 391, 1111, 1122
PciSI GCTCTTC 2 cut(s) 357, 500
PfeI GAWTC 3 cut(s) 461, 922, 1163
PfoI TCCNGGA 2 cut(s) 447, 547
PkrI GCNGC 3 cut(s) 69, 474, 973
PleI GAGTC 2 cut(s) 83, 104
PpsI GAGTC 2 cut(s) 83, 104
Psp124BI GAGCTC 2 cut(s) 87, 495
Psp6I CCWGG 2 cut(s) 400, 895
PspFI CCCAGC 1 cut(s) 1070
PspGI CCWGG 2 cut(s) 400, 895
PspN4I GGNNCC 1 cut(s) 760
PspPI GGNCC 3 cut(s) 248, 542, 759
PsuI RGATCY 4 cut(s) 397, 444, 877, 1045
RsaI GTAC 1 cut(s) 641
RsaNI GTAC 1 cut(s) 640
RseI CAYNNNNRTG 2 cut(s) 713, 1179
SacI GAGCTC 2 cut(s) 87, 495
SalI GTCGAC 1 cut(s) 7
SapI GCTCTTC 2 cut(s) 357, 500
SaqAI TTAA 3 cut(s) 804, 960, 1035
SatI GCNGC 3 cut(s) 68, 473, 972
Sau3AI GATC 9 cut(s) 367, 397, 444, 691, 723, 823, 877, 1045, 1083
Sau96I GGNCC 3 cut(s) 248, 542, 759
SchI GAGTC 2 cut(s) 83, 105
ScrFI CCNGG 4 cut(s) 402, 449, 549, 897
SduI GDGCHC 5 cut(s) 87, 308, 352, 495, 1174
SfaNI GCATC 3 cut(s) 519, 586, 718
SfcI CTRYAG 1 cut(s) 465
SinI GGWCC 2 cut(s) 542, 759
SmiMI CAYNNNNRTG 2 cut(s) 713, 1179
Sse9I AATT 5 cut(s) 579, 954, 963, 996, 1139
SsiI CCGC 6 cut(s) 42, 100, 109, 302, 420, 1051
SspMI CTAG 4 cut(s) 252, 434, 569, 905
SstI GAGCTC 2 cut(s) 87, 495
StyD4I CCNGG 4 cut(s) 400, 447, 547, 895
StyI CCWWGG 2 cut(s) 251, 522
TaaI ACNGT 2 cut(s) 773, 942
TaiI ACGT 2 cut(s) 8, 37
TaqI TCGA 5 cut(s) 8, 87, 213, 726, 918
TaqII GACCGA 1 cut(s) 26
TasI AATT 5 cut(s) 579, 954, 963, 996, 1139
TatI WGTACW 1 cut(s) 639
TfiI GAWTC 3 cut(s) 461, 922, 1163
Tru1I TTAA 3 cut(s) 804, 960, 1035
Tru9I TTAA 3 cut(s) 804, 960, 1035
TscAI CASTG 3 cut(s) 111, 769, 1069
TseI GCWGC 3 cut(s) 67, 472, 971
TspGWI ACGGA 1 cut(s) 1076
TspRI CASTG 3 cut(s) 111, 769, 1069
VneI GTGCAC 1 cut(s) 1170
VpaK11BI GGWCC 2 cut(s) 542, 759
XapI RAATTY 2 cut(s) 954, 963
XmaJI CCTAGG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 8
XspI CTAG 4 cut(s) 252, 434, 569, 905
ZraI GACGTC 2 cut(s) 6, 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.