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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 66 |
| AcoI | YGGCCR | 2 cut(s) | 107, 157 |
| AcsI | RAATTY | 1 cut(s) | 36 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 76, 166 |
| AoxI | GGCC | 3 cut(s) | 107, 138, 157 |
| ApeKI | GCWGC | 1 cut(s) | 60 |
| ApoI | RAATTY | 1 cut(s) | 36 |
| AxyI | CCTNAGG | 1 cut(s) | 31 |
| BalI | TGGCCA | 1 cut(s) | 159 |
| BbvI | GCAGC | 1 cut(s) | 72 |
| BccI | CCATC | 1 cut(s) | 198 |
| BfaI | CTAG | 1 cut(s) | 199 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 146 |
| BisI | GCNGC | 1 cut(s) | 61 |
| BlsI | GCNGC | 1 cut(s) | 62 |
| BsaJI | CCNNGG | 1 cut(s) | 165 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 76, 166 |
| Bse118I | RCCGGY | 1 cut(s) | 109 |
| Bse21I | CCTNAGG | 1 cut(s) | 31 |
| BseDI | CCNNGG | 1 cut(s) | 165 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 76, 166 |
| BseMII | CTCAG | 1 cut(s) | 45 |
| BseXI | GCAGC | 1 cut(s) | 72 |
| BshFI | GGCC | 3 cut(s) | 109, 140, 159 |
| BsiSI | CCGG | 2 cut(s) | 110, 125 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 76, 166 |
| BsnI | GGCC | 3 cut(s) | 109, 140, 159 |
| BspACI | CCGC | 1 cut(s) | 66 |
| BspANI | GGCC | 3 cut(s) | 109, 140, 159 |
| BspCNI | CTCAG | 1 cut(s) | 44 |
| BsrFI | RCCGGY | 1 cut(s) | 109 |
| BssAI | RCCGGY | 1 cut(s) | 109 |
| BssECI | CCNNGG | 1 cut(s) | 165 |
| BstDEI | CTNAG | 1 cut(s) | 31 |
| BstDSI | CCRYGG | 1 cut(s) | 165 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 146 |
| BstV1I | GCAGC | 1 cut(s) | 72 |
| Bsu36I | CCTNAGG | 1 cut(s) | 31 |
| BsuRI | GGCC | 3 cut(s) | 109, 140, 159 |
| BtgI | CCRYGG | 1 cut(s) | 165 |
| Cfr10I | RCCGGY | 1 cut(s) | 109 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 161, 196 |
| CviJI | RGCY | 4 cut(s) | 90, 109, 140, 159 |
| CviKI_1 | RGCY | 4 cut(s) | 90, 109, 140, 159 |
| DdeI | CTNAG | 1 cut(s) | 31 |
| EaeI | YGGCCR | 2 cut(s) | 107, 157 |
| Eco147I | AGGCCT | 1 cut(s) | 140 |
| Eco81I | CCTNAGG | 1 cut(s) | 31 |
| Fnu4HI | GCNGC | 1 cut(s) | 61 |
| Fsp4HI | GCNGC | 1 cut(s) | 61 |
| FspBI | CTAG | 1 cut(s) | 199 |
| GluI | GCNGC | 1 cut(s) | 61 |
| HaeIII | GGCC | 3 cut(s) | 109, 140, 159 |
| HapII | CCGG | 2 cut(s) | 110, 125 |
| HpaII | CCGG | 2 cut(s) | 110, 125 |
| Hpy188III | TCNNGA | 1 cut(s) | 33 |
| Hpy99I | CGWCG | 1 cut(s) | 58 |
| HpyAV | CCTTC | 2 cut(s) | 142, 151 |
| HpyCH4IV | ACGT | 2 cut(s) | 8, 46 |
| HpyCH4V | TGCA | 1 cut(s) | 117 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 146 |
| HpyF3I | CTNAG | 1 cut(s) | 31 |
| HpySE526I | ACGT | 2 cut(s) | 8, 46 |
| LpnPI | CCDG | 5 cut(s) | 18, 100, 123, 138, 141 |
| Lsp1109I | GCAGC | 1 cut(s) | 72 |
| MaeI | CTAG | 1 cut(s) | 199 |
| MaeII | ACGT | 2 cut(s) | 8, 46 |
| MboII | GAAGA | 2 cut(s) | 32, 185 |
| MlsI | TGGCCA | 1 cut(s) | 159 |
| MluCI | AATT | 3 cut(s) | 36, 81, 173 |
| MluNI | TGGCCA | 1 cut(s) | 159 |
| MnlI | CCTC | 4 cut(s) | 40, 70, 130, 139 |
| Mox20I | TGGCCA | 1 cut(s) | 159 |
| MscI | TGGCCA | 1 cut(s) | 159 |
| Msp20I | TGGCCA | 1 cut(s) | 159 |
| MspI | CCGG | 2 cut(s) | 110, 125 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 146 |
| PceI | AGGCCT | 1 cut(s) | 140 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 141 |
| PkrI | GCNGC | 1 cut(s) | 62 |
| SatI | GCNGC | 1 cut(s) | 61 |
| SetI | ASST | 4 cut(s) | 11, 32, 49, 134 |
| Sse9I | AATT | 3 cut(s) | 36, 81, 173 |
| SseBI | AGGCCT | 1 cut(s) | 140 |
| SsiI | CCGC | 1 cut(s) | 66 |
| SspMI | CTAG | 1 cut(s) | 199 |
| StuI | AGGCCT | 1 cut(s) | 140 |
| TaiI | ACGT | 2 cut(s) | 11, 49 |
| TaqI | TCGA | 3 cut(s) | 53, 135, 144 |
| TasI | AATT | 3 cut(s) | 36, 81, 173 |
| TseI | GCWGC | 1 cut(s) | 60 |
| XapI | RAATTY | 1 cut(s) | 36 |
| XspI | CTAG | 1 cut(s) | 199 |