pycom08g10800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002872
Physical Location & Seq
Reverse (-)
9043846 .. 9045819
1974 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g10800.5

Sequence Viewer

Length: 600 bp
ATGTTTATCCCAAGGGGCCCACCCCCTGTTTTTGTTCCCCGGTTCTTTCCCTTTTCTTTTTTTTCTGAAAAGTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCTTTCTCTTTGTCTTCCACTCCTTCCTCCGACAAGAACTCACACACACACACACCCATGCCCACACCTATAACTCTTTTCTAACCCCGTGGTTGTGTTTTGGACGTCAAACCATGAAGAAAACCACCTCGGAGACATTTCCCAGTTTTCCGGCGAGCACCGCCCCTTTCGAGGTAATTTCCGGCCAAACCACGGCGAGTTGGCCGGCGTGCGAGTTTCCGGCGGACACCCGTGGCTTCCAGGCTGTTTTCCGGCCAACTCCGACCACGATGACGGGAACCAAGGGGGCGTTTAATTGCGGCGTTTCCTTCTTCGCTAGGTGGCGTGCCGGCATGGGGCCTGAAGTGTTATATCGGACATTCACTAGTGACTTAGAATCAAGGCATATAATCCCGACGTCCAGGCACTTCTGCATCGGCATCTTCGAGTCCTCTCGGTGTAGGACCCACTTCTTTGTTTCATTCAATTTTATATTATTTTCTTTTAGTGTTCTAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

22.74

Weight (kDa)

9.44

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 208, 500
AciI CCGC 3 cut(s) 261, 323, 399
AcoI YGGCCR 3 cut(s) 283, 302, 353
AcuI CTGAAG 1 cut(s) 462
AcyI GRCGYC 2 cut(s) 205, 497
AfiI CCNNNNNNNGG 3 cut(s) 271, 292, 435
AgsI TTSAA 1 cut(s) 565
AhlI ACTAGT 1 cut(s) 464
AjnI CCWGG 2 cut(s) 339, 500
Alw21I GWGCWC 1 cut(s) 260
Alw26I GTCTC 2 cut(s) 78, 227
AoxI GGCC 5 cut(s) 16, 283, 302, 353, 437
ApaI GGGCCC 1 cut(s) 20
AspS9I GGNCC 4 cut(s) 16, 17, 437, 543
AsuC2I CCSGG 1 cut(s) 40
AvaII GGWCC 1 cut(s) 543
BaeGI GKGCMC 1 cut(s) 20
BanII GRGCYC 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 260
BceAI ACGGC 1 cut(s) 309
BciT130I CCWGG 2 cut(s) 341, 502
BcnI CCSGG 1 cut(s) 40
BcoDI GTCTC 2 cut(s) 78, 227
BcuI ACTAGT 1 cut(s) 464
BfaI CTAG 3 cut(s) 417, 465, 593
BisI GCNGC 1 cut(s) 400
BlsI GCNGC 1 cut(s) 401
Bme1390I CCNGG 3 cut(s) 40, 341, 502
Bme18I GGWCC 1 cut(s) 543
BmgT120I GGNCC 4 cut(s) 16, 17, 437, 543
BmiI GGNNCC 5 cut(s) 17, 18, 379, 438, 545
BmrFI CCNGG 3 cut(s) 40, 341, 502
BmrI ACTGGG 1 cut(s) 237
BmsI GCATC 2 cut(s) 522, 528
BmuI ACTGGG 1 cut(s) 237
BpiI GAAGAC 1 cut(s) 106
BpuMI CCSGG 1 cut(s) 40
BsaHI GRCGYC 2 cut(s) 205, 497
BsaJI CCNNGG 7 cut(s) 11, 38, 188, 228, 291, 331, 381
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 292, 435
Bse118I RCCGGY 2 cut(s) 304, 428
Bse1I ACTGG 1 cut(s) 243
BseBI CCWGG 2 cut(s) 341, 502
BseDI CCNNGG 7 cut(s) 11, 38, 188, 228, 291, 331, 381
BseLI CCNNNNNNNGG 3 cut(s) 271, 292, 435
BseNI ACTGG 1 cut(s) 243
BseSI GKGCMC 1 cut(s) 20
BshFI GGCC 5 cut(s) 18, 285, 304, 355, 439
BsiHKAI GWGCWC 1 cut(s) 260
BsiSI CCGG 7 cut(s) 40, 251, 282, 305, 320, 352, 429
BslI CCNNNNNNNGG 3 cut(s) 271, 292, 435
BsmAI GTCTC 2 cut(s) 78, 227
BsnI GGCC 5 cut(s) 18, 285, 304, 355, 439
Bsp120I GGGCCC 1 cut(s) 16
Bsp1286I GDGCHC 2 cut(s) 20, 260
BspACI CCGC 3 cut(s) 261, 323, 399
BspANI GGCC 5 cut(s) 18, 285, 304, 355, 439
BspLI GGNNCC 5 cut(s) 17, 18, 379, 438, 545
BsrFI RCCGGY 2 cut(s) 304, 428
BsrI ACTGG 1 cut(s) 243
BssAI RCCGGY 2 cut(s) 304, 428
BssECI CCNNGG 7 cut(s) 11, 38, 188, 228, 291, 331, 381
BssNI GRCGYC 2 cut(s) 205, 497
BssT1I CCWWGG 2 cut(s) 11, 381
Bst2UI CCWGG 2 cut(s) 341, 502
BstACI GRCGYC 2 cut(s) 205, 497
BstC8I GCNNGC 5 cut(s) 256, 306, 310, 426, 430
BstDEI CTNAG 1 cut(s) 472
BstDSI CCRYGG 3 cut(s) 188, 291, 331
BstMAI GTCTC 2 cut(s) 78, 227
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstNI CCWGG 2 cut(s) 341, 502
BstSCI CCNGG 3 cut(s) 38, 339, 500
BstSLI GKGCMC 1 cut(s) 20
BstV2I GAAGAC 1 cut(s) 106
BsuRI GGCC 5 cut(s) 18, 285, 304, 355, 439
BtgI CCRYGG 3 cut(s) 188, 291, 331
Cac8I GCNNGC 5 cut(s) 256, 306, 310, 426, 430
Cfr10I RCCGGY 2 cut(s) 304, 428
Cfr13I GGNCC 4 cut(s) 16, 17, 437, 543
CviAII CATG 3 cut(s) 158, 214, 433
CviJI RGCY 7 cut(s) 18, 285, 304, 336, 344, 355, 439
CviKI_1 RGCY 7 cut(s) 18, 285, 304, 336, 344, 355, 439
DdeI CTNAG 1 cut(s) 472
EaeI YGGCCR 3 cut(s) 283, 302, 353
EciI GGCGGA 1 cut(s) 338
Eco130I CCWWGG 2 cut(s) 11, 381
Eco24I GRGCYC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 543
Eco57I CTGAAG 1 cut(s) 462
EcoO109I RGGNCCY 3 cut(s) 16, 437, 543
EcoRII CCWGG 2 cut(s) 339, 500
EcoT14I CCWWGG 2 cut(s) 11, 381
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 2 cut(s) 11, 381
FaeI CATG 3 cut(s) 161, 217, 436
FaiI YATR 8 cut(s) 159, 171, 215, 434, 451, 486, 488, 572
FatI CATG 3 cut(s) 157, 213, 432
Fnu4HI GCNGC 1 cut(s) 400
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 1 cut(s) 400
FspBI CTAG 3 cut(s) 417, 465, 593
GluI GCNGC 1 cut(s) 400
HaeIII GGCC 5 cut(s) 18, 285, 304, 355, 439
HapII CCGG 7 cut(s) 40, 251, 282, 305, 320, 352, 429
Hin1I GRCGYC 2 cut(s) 205, 497
Hin1II CATG 3 cut(s) 161, 217, 436
HinfI GANTC 2 cut(s) 476, 527
HpaII CCGG 7 cut(s) 40, 251, 282, 305, 320, 352, 429
Hpy188I TCNGA 5 cut(s) 67, 131, 232, 363, 456
Hpy188III TCNNGA 2 cut(s) 493, 593
Hpy99I CGWCG 1 cut(s) 499
HpyAV CCTTC 2 cut(s) 133, 418
HpyCH4IV ACGT 2 cut(s) 205, 497
HpyCH4V TGCA 1 cut(s) 513
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpyF3I CTNAG 1 cut(s) 472
HpySE526I ACGT 2 cut(s) 205, 497
Hsp92I GRCGYC 2 cut(s) 205, 497
Hsp92II CATG 3 cut(s) 161, 217, 436
KroI GCCGGC 2 cut(s) 304, 428
KroNI GCCGGC 2 cut(s) 306, 430
LweI GCATC 2 cut(s) 522, 528
MaeI CTAG 3 cut(s) 417, 465, 593
MaeII ACGT 2 cut(s) 205, 497
MaeIII GTNAC 1 cut(s) 467
MboII GAAGA 4 cut(s) 106, 229, 403, 514
MhlI GDGCHC 2 cut(s) 20, 260
MluCI AATT 3 cut(s) 276, 394, 565
MlyI GAGTC 1 cut(s) 536
MmeI TCCRAC 2 cut(s) 154, 386
MnlI CCTC 4 cut(s) 137, 238, 265, 541
MroNI GCCGGC 2 cut(s) 304, 428
MseI TTAA 1 cut(s) 393
MslI CAYNNNNRTG 1 cut(s) 156
MspI CCGG 7 cut(s) 40, 251, 282, 305, 320, 352, 429
MspR9I CCNGG 3 cut(s) 40, 341, 502
MvaI CCWGG 2 cut(s) 341, 502
MwoI GCNNNNNNNGC 1 cut(s) 260
NaeI GCCGGC 2 cut(s) 306, 430
NciI CCSGG 1 cut(s) 40
NgoMIV GCCGGC 2 cut(s) 304, 428
NlaIII CATG 3 cut(s) 161, 217, 436
NlaIV GGNNCC 5 cut(s) 17, 18, 379, 438, 545
NmuCI GTSAC 1 cut(s) 467
PcsI WCGNNNNNNNCGW 1 cut(s) 522
PdiI GCCGGC 2 cut(s) 306, 430
PfeI GAWTC 1 cut(s) 476
PkrI GCNGC 1 cut(s) 401
PleI GAGTC 1 cut(s) 535
PpsI GAGTC 1 cut(s) 535
PpuMI RGGWCCY 1 cut(s) 543
Psp5II RGGWCCY 1 cut(s) 543
Psp6I CCWGG 2 cut(s) 339, 500
PspGI CCWGG 2 cut(s) 339, 500
PspN4I GGNNCC 5 cut(s) 17, 18, 379, 438, 545
PspOMI GGGCCC 1 cut(s) 16
PspPI GGNCC 4 cut(s) 16, 17, 437, 543
PspPPI RGGWCCY 1 cut(s) 543
RseI CAYNNNNRTG 1 cut(s) 156
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 1 cut(s) 400
Sau96I GGNCC 4 cut(s) 16, 17, 437, 543
SchI GAGTC 1 cut(s) 536
ScrFI CCNGG 3 cut(s) 40, 341, 502
SduI GDGCHC 2 cut(s) 20, 260
SetI ASST 6 cut(s) 170, 208, 230, 276, 422, 500
SfaNI GCATC 2 cut(s) 522, 528
SinI GGWCC 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 156
SpeI ACTAGT 1 cut(s) 464
Sse9I AATT 3 cut(s) 276, 394, 565
SsiI CCGC 3 cut(s) 261, 323, 399
SspMI CTAG 3 cut(s) 417, 465, 593
StyD4I CCNGG 3 cut(s) 38, 339, 500
StyI CCWWGG 2 cut(s) 11, 381
TaiI ACGT 2 cut(s) 208, 500
TaqI TCGA 2 cut(s) 270, 525
TasI AATT 3 cut(s) 276, 394, 565
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TseFI GTSAC 1 cut(s) 467
Tsp45I GTSAC 1 cut(s) 467
TspDTI ATGAA 2 cut(s) 230, 549
VpaK11BI GGWCC 1 cut(s) 543
XbaI TCTAGA 1 cut(s) 592
XspI CTAG 3 cut(s) 417, 465, 593
ZraI GACGTC 2 cut(s) 206, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.