pycom04g19170

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002872
Physical Location & Seq
Forward (+)
20936558 .. 20937406
849 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g19170.4

Sequence Viewer

Length: 525 bp
ATGGTTCACAAACATGATCGTAATAACCCGTCCCGGGATTTACTGAAGAAAATGGGTATTTTTGTATTTTCACTAATGGTGAACCCAAGGGGCCCACCCCCTGTTTTGTCCACCCTGCCGTGTCCCTCCTCTCTTCTTTCTTCTTTTTCTTTTTTTCTTCTTTCCTTCTTTTCTTTCGTTGTCTGCACTTTTTTTTCCTTTCTTTTTGAAAACTCCTCCATTCTTCTCTTCTTCTTCCCCGTGACAACACACACACTCACAACTACACCTACTCGAGGAAATCATCACCATCATCGCCATTCAACTGCATCTCTCTCTCTCTCTCTTTCCCCTGCTCGCGGCGCCGGGAGGACACAAACACCTCCGGCGAGTTTTGGTTGTTTTCCGATTGGGTTTTGGAGTGTTTTTGGCACGGGTGAGTGCACACCCAATTTTCCGGCGAGCACCGCCCCTTTCGAGGAAATTTCCGGCCAAACCACGGCGAGTTGGCCGGCGTGCAAGGTATCAATCTCTTCGTCTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

19.07

Weight (kDa)

9.56

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 341
AccII CGCG 1 cut(s) 339
AciI CCGC 2 cut(s) 339, 447
AcoI YGGCCR 2 cut(s) 469, 488
AcsI RAATTY 1 cut(s) 462
AcuI CTGAAG 1 cut(s) 65
AcyI GRCGYC 1 cut(s) 342
AfiI CCNNNNNNNGG 5 cut(s) 34, 275, 338, 457, 478
AgsI TTSAA 2 cut(s) 209, 303
Alw21I GWGCWC 2 cut(s) 425, 446
Alw44I GTGCAC 1 cut(s) 421
Ama87I CYCGRG 2 cut(s) 33, 273
AoxI GGCC 3 cut(s) 91, 469, 488
ApaI GGGCCC 1 cut(s) 95
ApaLI GTGCAC 1 cut(s) 421
ApoI RAATTY 1 cut(s) 462
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 2 cut(s) 91, 92
AsuC2I CCSGG 3 cut(s) 34, 35, 346
AsuHPI GGTGA 3 cut(s) 91, 278, 428
AvaI CYCGRG 2 cut(s) 33, 273
BaeGI GKGCMC 2 cut(s) 95, 425
BanI GGYRCC 1 cut(s) 341
BanII GRGCYC 1 cut(s) 95
Bbv12I GWGCWC 2 cut(s) 425, 446
BccI CCATC 1 cut(s) 297
BceAI ACGGC 2 cut(s) 103, 495
BcnI CCSGG 3 cut(s) 34, 35, 346
BfoI RGCGCY 1 cut(s) 345
BisI GCNGC 1 cut(s) 340
BlsI GCNGC 1 cut(s) 341
Bme1390I CCNGG 3 cut(s) 34, 35, 346
BmeT110I CYCGRG 2 cut(s) 33, 273
BmgT120I GGNCC 2 cut(s) 91, 92
BmiI GGNNCC 3 cut(s) 92, 93, 343
BmrFI CCNGG 3 cut(s) 34, 35, 346
BmsI GCATC 1 cut(s) 317
BpuMI CCSGG 3 cut(s) 34, 35, 346
BsaHI GRCGYC 1 cut(s) 342
BsaJI CCNNGG 3 cut(s) 33, 86, 477
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 275, 338, 457, 478
Bse118I RCCGGY 1 cut(s) 490
BseDI CCNNGG 3 cut(s) 33, 86, 477
BseLI CCNNNNNNNGG 5 cut(s) 34, 275, 338, 457, 478
BseRI GAGGAG 2 cut(s) 118, 205
BseSI GKGCMC 2 cut(s) 95, 425
BsgI GTGCAG 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 339
BshFI GGCC 3 cut(s) 93, 471, 490
BshNI GGYRCC 1 cut(s) 341
BsiHKAI GWGCWC 2 cut(s) 425, 446
BsiHKCI CYCGRG 2 cut(s) 33, 273
BsiSI CCGG 6 cut(s) 34, 345, 365, 437, 468, 491
BslFI GGGAC 2 cut(s) 16, 108
BslI CCNNNNNNNGG 5 cut(s) 34, 275, 338, 457, 478
BsmFI GGGAC 2 cut(s) 16, 108
BsnI GGCC 3 cut(s) 93, 471, 490
BsoBI CYCGRG 2 cut(s) 33, 273
Bsp120I GGGCCC 1 cut(s) 91
Bsp1286I GDGCHC 3 cut(s) 95, 425, 446
Bsp143I GATC 1 cut(s) 16
BspACI CCGC 2 cut(s) 339, 447
BspANI GGCC 3 cut(s) 93, 471, 490
BspFNI CGCG 1 cut(s) 339
BspLI GGNNCC 3 cut(s) 92, 93, 343
BspT107I GGYRCC 1 cut(s) 341
BsrFI RCCGGY 1 cut(s) 490
BssAI RCCGGY 1 cut(s) 490
BssECI CCNNGG 3 cut(s) 33, 86, 477
BssMI GATC 1 cut(s) 16
BssNI GRCGYC 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 86
Bst6I CTCTTC 3 cut(s) 138, 233, 517
BstACI GRCGYC 1 cut(s) 342
BstC8I GCNNGC 4 cut(s) 337, 442, 492, 496
BstDSI CCRYGG 1 cut(s) 477
BstENI CCTNNNNNAGG 1 cut(s) 273
BstFNI CGCG 1 cut(s) 339
BstH2I RGCGCY 1 cut(s) 345
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 1 cut(s) 19
BstMBI GATC 1 cut(s) 16
BstMWI GCNNNNNNNGC 2 cut(s) 341, 446
BstSCI CCNGG 3 cut(s) 32, 33, 344
BstSLI GKGCMC 2 cut(s) 95, 425
BstUI CGCG 1 cut(s) 339
BsuRI GGCC 3 cut(s) 93, 471, 490
BtgI CCRYGG 1 cut(s) 477
BtgZI GCGATG 1 cut(s) 278
Cac8I GCNNGC 4 cut(s) 337, 442, 492, 496
CfoI GCGC 1 cut(s) 344
Cfr10I RCCGGY 1 cut(s) 490
Cfr13I GGNCC 2 cut(s) 91, 92
Cfr9I CCCGGG 1 cut(s) 33
CviAII CATG 1 cut(s) 14
CviJI RGCY 3 cut(s) 93, 471, 490
CviKI_1 RGCY 3 cut(s) 93, 471, 490
DinI GGCGCC 1 cut(s) 343
DpnI GATC 1 cut(s) 18
DpnII GATC 1 cut(s) 16
EaeI YGGCCR 2 cut(s) 469, 488
Eam1104I CTCTTC 3 cut(s) 138, 233, 517
EarI CTCTTC 3 cut(s) 138, 233, 517
Eco130I CCWWGG 1 cut(s) 86
Eco24I GRGCYC 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 65
Eco88I CYCGRG 2 cut(s) 33, 273
EcoNI CCTNNNNNAGG 1 cut(s) 273
EcoO109I RGGNCCY 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 86
EcoT38I GRGCYC 1 cut(s) 95
EgeI GGCGCC 1 cut(s) 343
EheI GGCGCC 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 86
FaeI CATG 1 cut(s) 17
FaiI YATR 1 cut(s) 15
FaqI GGGAC 2 cut(s) 16, 108
FatI CATG 1 cut(s) 13
Fnu4HI GCNGC 1 cut(s) 340
FriOI GRGCYC 1 cut(s) 95
Fsp4HI GCNGC 1 cut(s) 340
GlaI GCGC 1 cut(s) 343
GluI GCNGC 1 cut(s) 340
HaeII RGCGCY 1 cut(s) 345
HaeIII GGCC 3 cut(s) 93, 471, 490
HapII CCGG 6 cut(s) 34, 345, 365, 437, 468, 491
HhaI GCGC 1 cut(s) 344
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 1 cut(s) 17
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HpaII CCGG 6 cut(s) 34, 345, 365, 437, 468, 491
HphI GGTGA 3 cut(s) 91, 278, 428
Hpy166II GTNNAC 4 cut(s) 7, 82, 111, 423
Hpy188I TCNGA 1 cut(s) 387
Hpy8I GTNNAC 4 cut(s) 7, 82, 111, 423
HpyAV CCTTC 1 cut(s) 175
HpyCH4V TGCA 4 cut(s) 186, 308, 423, 498
HpyF10VI GCNNNNNNNGC 2 cut(s) 341, 446
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 1 cut(s) 17
HspAI GCGC 1 cut(s) 342
KasI GGCGCC 1 cut(s) 341
KroI GCCGGC 1 cut(s) 490
KroNI GCCGGC 1 cut(s) 492
Kzo9I GATC 1 cut(s) 16
LpnPI CCDG 9 cut(s) 47, 114, 128, 345, 358, 378, 450, 481, 504
LweI GCATC 1 cut(s) 317
MaeIII GTNAC 1 cut(s) 241
MalI GATC 1 cut(s) 18
MboI GATC 1 cut(s) 16
MboII GAAGA 9 cut(s) 58, 125, 132, 149, 215, 220, 223, 226, 504
MhlI GDGCHC 3 cut(s) 95, 425, 446
MluCI AATT 2 cut(s) 430, 462
Mly113I GGCGCC 1 cut(s) 342
MnlI CCTC 7 cut(s) 136, 139, 226, 269, 342, 372, 451
MroNI GCCGGC 1 cut(s) 490
MslI CAYNNNNRTG 1 cut(s) 12
MspI CCGG 6 cut(s) 34, 345, 365, 437, 468, 491
MspR9I CCNGG 3 cut(s) 34, 35, 346
MvnI CGCG 1 cut(s) 339
MwoI GCNNNNNNNGC 2 cut(s) 341, 446
NaeI GCCGGC 1 cut(s) 492
NarI GGCGCC 1 cut(s) 342
NciI CCSGG 3 cut(s) 34, 35, 346
NdeII GATC 1 cut(s) 16
NgoMIV GCCGGC 1 cut(s) 490
NlaIII CATG 1 cut(s) 17
NlaIV GGNNCC 3 cut(s) 92, 93, 343
NmuCI GTSAC 1 cut(s) 241
PaeR7I CTCGAG 1 cut(s) 273
PdiI GCCGGC 1 cut(s) 492
PkrI GCNGC 1 cut(s) 341
PluTI GGCGCC 1 cut(s) 345
PspN4I GGNNCC 3 cut(s) 92, 93, 343
PspOMI GGGCCC 1 cut(s) 91
PspPI GGNCC 2 cut(s) 91, 92
PspXI VCTCGAGB 1 cut(s) 273
RseI CAYNNNNRTG 1 cut(s) 12
SatI GCNGC 1 cut(s) 340
Sau3AI GATC 1 cut(s) 16
Sau96I GGNCC 2 cut(s) 91, 92
ScrFI CCNGG 3 cut(s) 34, 35, 346
SduI GDGCHC 3 cut(s) 95, 425, 446
SetI ASST 3 cut(s) 271, 364, 504
SfaNI GCATC 1 cut(s) 317
SfoI GGCGCC 1 cut(s) 343
Sfr274I CTCGAG 1 cut(s) 273
SlaI CTCGAG 1 cut(s) 273
SmaI CCCGGG 1 cut(s) 35
SmiMI CAYNNNNRTG 1 cut(s) 12
SmlI CTYRAG 1 cut(s) 273
SmoI CTYRAG 1 cut(s) 273
Sse9I AATT 2 cut(s) 430, 462
SsiI CCGC 2 cut(s) 339, 447
SspDI GGCGCC 1 cut(s) 341
StyD4I CCNGG 3 cut(s) 32, 33, 344
StyI CCWWGG 1 cut(s) 86
TaqI TCGA 2 cut(s) 274, 456
TasI AATT 2 cut(s) 430, 462
TauI GCSGC 1 cut(s) 342
TseFI GTSAC 1 cut(s) 241
Tsp45I GTSAC 1 cut(s) 241
TspMI CCCGGG 1 cut(s) 33
VneI GTGCAC 1 cut(s) 421
XagI CCTNNNNNAGG 1 cut(s) 273
XapI RAATTY 1 cut(s) 462
XhoI CTCGAG 1 cut(s) 273
XmaI CCCGGG 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.