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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 116 |
| AccIII | TCCGGA | 1 cut(s) | 82 |
| AciI | CCGC | 1 cut(s) | 127 |
| AcyI | GRCGYC | 1 cut(s) | 113 |
| AfaI | GTAC | 1 cut(s) | 77 |
| AgsI | TTSAA | 1 cut(s) | 181 |
| Aor13HI | TCCGGA | 1 cut(s) | 82 |
| AspS9I | GGNCC | 1 cut(s) | 159 |
| AvaII | GGWCC | 1 cut(s) | 159 |
| BceAI | ACGGC | 1 cut(s) | 84 |
| BfaI | CTAG | 2 cut(s) | 48, 209 |
| BmcAI | AGTACT | 1 cut(s) | 77 |
| Bme18I | GGWCC | 1 cut(s) | 159 |
| BmgT120I | GGNCC | 1 cut(s) | 159 |
| BmiI | GGNNCC | 2 cut(s) | 10, 161 |
| BmsI | GCATC | 2 cut(s) | 138, 144 |
| BsaHI | GRCGYC | 1 cut(s) | 113 |
| BsaJI | CCNNGG | 1 cut(s) | 12 |
| BsaWI | WCCGGW | 1 cut(s) | 82 |
| BseAI | TCCGGA | 1 cut(s) | 82 |
| BseDI | CCNNGG | 1 cut(s) | 12 |
| BsiSI | CCGG | 1 cut(s) | 83 |
| Bsp13I | TCCGGA | 1 cut(s) | 82 |
| BspACI | CCGC | 1 cut(s) | 127 |
| BspEI | TCCGGA | 1 cut(s) | 82 |
| BspLI | GGNNCC | 2 cut(s) | 10, 161 |
| BssECI | CCNNGG | 1 cut(s) | 12 |
| BssNI | GRCGYC | 1 cut(s) | 113 |
| BssT1I | CCWWGG | 1 cut(s) | 12 |
| BstACI | GRCGYC | 1 cut(s) | 113 |
| BstDEI | CTNAG | 1 cut(s) | 88 |
| Cfr13I | GGNCC | 1 cut(s) | 159 |
| Csp6I | GTAC | 1 cut(s) | 76 |
| CviJI | RGCY | 1 cut(s) | 60 |
| CviKI_1 | RGCY | 1 cut(s) | 60 |
| CviQI | GTAC | 1 cut(s) | 76 |
| DdeI | CTNAG | 1 cut(s) | 88 |
| Eco130I | CCWWGG | 1 cut(s) | 12 |
| Eco47I | GGWCC | 1 cut(s) | 159 |
| EcoO109I | RGGNCCY | 1 cut(s) | 159 |
| EcoT14I | CCWWGG | 1 cut(s) | 12 |
| ErhI | CCWWGG | 1 cut(s) | 12 |
| FaiI | YATR | 2 cut(s) | 102, 188 |
| FspBI | CTAG | 2 cut(s) | 48, 209 |
| HapII | CCGG | 1 cut(s) | 83 |
| Hin1I | GRCGYC | 1 cut(s) | 113 |
| HinfI | GANTC | 1 cut(s) | 92 |
| HpaII | CCGG | 1 cut(s) | 83 |
| Hpy188III | TCNNGA | 4 cut(s) | 83, 109, 117, 209 |
| Hpy99I | CGWCG | 2 cut(s) | 70, 115 |
| HpyAV | CCTTC | 1 cut(s) | 49 |
| HpyCH4IV | ACGT | 1 cut(s) | 113 |
| HpyF3I | CTNAG | 1 cut(s) | 88 |
| HpySE526I | ACGT | 1 cut(s) | 113 |
| Hsp92I | GRCGYC | 1 cut(s) | 113 |
| Kpn2I | TCCGGA | 1 cut(s) | 82 |
| LpnPI | CCDG | 2 cut(s) | 96, 130 |
| LweI | GCATC | 2 cut(s) | 138, 144 |
| MaeI | CTAG | 2 cut(s) | 48, 209 |
| MaeII | ACGT | 1 cut(s) | 113 |
| MboII | GAAGA | 2 cut(s) | 34, 130 |
| MluCI | AATT | 1 cut(s) | 181 |
| MnlI | CCTC | 1 cut(s) | 90 |
| MroI | TCCGGA | 1 cut(s) | 82 |
| MspI | CCGG | 1 cut(s) | 83 |
| NlaIV | GGNNCC | 2 cut(s) | 10, 161 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 138 |
| PfeI | GAWTC | 1 cut(s) | 92 |
| PpuMI | RGGWCCY | 1 cut(s) | 159 |
| Psp5II | RGGWCCY | 1 cut(s) | 159 |
| PspN4I | GGNNCC | 2 cut(s) | 10, 161 |
| PspPI | GGNCC | 1 cut(s) | 159 |
| PspPPI | RGGWCCY | 1 cut(s) | 159 |
| RsaI | GTAC | 1 cut(s) | 77 |
| RsaNI | GTAC | 1 cut(s) | 76 |
| Sau96I | GGNCC | 1 cut(s) | 159 |
| ScaI | AGTACT | 1 cut(s) | 77 |
| SetI | ASST | 2 cut(s) | 53, 116 |
| SfaNI | GCATC | 2 cut(s) | 138, 144 |
| SinI | GGWCC | 1 cut(s) | 159 |
| Sse9I | AATT | 1 cut(s) | 181 |
| SsiI | CCGC | 1 cut(s) | 127 |
| SspMI | CTAG | 2 cut(s) | 48, 209 |
| StyI | CCWWGG | 1 cut(s) | 12 |
| TaiI | ACGT | 1 cut(s) | 116 |
| TaqI | TCGA | 3 cut(s) | 65, 95, 141 |
| TasI | AATT | 1 cut(s) | 181 |
| TatI | WGTACW | 1 cut(s) | 75 |
| TfiI | GAWTC | 1 cut(s) | 92 |
| TspDTI | ATGAA | 1 cut(s) | 165 |
| VpaK11BI | GGWCC | 1 cut(s) | 159 |
| XbaI | TCTAGA | 1 cut(s) | 208 |
| XspI | CTAG | 2 cut(s) | 48, 209 |
| ZraI | GACGTC | 1 cut(s) | 114 |
| ZrmI | AGTACT | 1 cut(s) | 77 |