pycom02g19260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002872
Physical Location & Seq
Forward (+)
17174834 .. 17177046
2213 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g19260.3

Sequence Viewer

Length: 918 bp
ATGCAGATACAGGTGTTTGCTCAGCAAGTTTGGTGGCCATGGTGCAATCAGCAGCTAAGCCTAAGAGTTTATTGTTTTGTTGTTAAGGTTTTACTGAAATTACTCAAACATTCTCAACTAACGGGTGCTAACGTTTTTGTATTTTCATTAATGGTAAGCCAAGAGGCCCACCCCCTGTTTTTCTTCTCTTCTCTCTTCTCTCTTTCTTCCTCCGTGCTCTTTCTTTCCCCCCTCCTCTCGGTGCTCTTTCCTCCGACAAGAACACACACACACACACACACCCATGCCCACACCTATAGCTCTTTTCTAACCCCGTGGTTGTGTTTTGGACGTCAAACCATGAAGAAATCACCTCGGAGACATTTCCCAGTTTTCCGGCGCGCACCGCCCCTTTCGAGGCAATTTGCGGCCAAACCACGGCGATTTGGCCGGCGTGCGAGGTATCAATCTCTTTGTCTCGCTGAGTACTACAACTTTTCTTTTTTTGAATCTTACCCAGTTCCGGCGGACACCCGTGGCTTCCAGGCTGTTTTCCGGCCAACTCCGACCACAATGACCGGAACCAAGGTTAGGCCTTGGGCCGCTAATTTTGACGTTCTGAACCCGTTTTCGATGTCCTGTGGCGTTTCCGTCTTCGCTAGTGGCGTGGCTTTTCGACGGCGAAGTACTGAAGTTGATTTTCAATATATGTATGTTCACTGGTTTTGTACTTACCTAGTGGTCCTTTCCGCTACGGGACGTAGGGATAGATCCGGTCCGTCCCGGGCGACGGTTTGGTGTTGGCATAGGGCCTGGAGTGTGTTTTCCTCTGACTGTCTGCTCAGAGACGGGGAGCCGGCAAGGGGACTTAGAATCGAGGCATACAATCCCGACGTCCAGGCACTTCCGCATCGGCATCTTCTAGTCCTCTCGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

306

Amino Acids

35.32

Weight (kDa)

10.45

Isoelectric Point (pI)

52.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 334, 876
AccII CGCG 1 cut(s) 381
AciI CCGC 6 cut(s) 386, 407, 506, 582, 729, 887
AclI AACGTT 1 cut(s) 132
AclWI GGATC 1 cut(s) 744
AcoI YGGCCR 4 cut(s) 35, 408, 427, 536
AcuI CTGAAG 1 cut(s) 690
AcyI GRCGYC 2 cut(s) 331, 873
AfaI GTAC 3 cut(s) 467, 667, 709
AfiI CCNNNNNNNGG 7 cut(s) 238, 396, 417, 502, 570, 769, 842
AgsI TTSAA 2 cut(s) 488, 683
AjnI CCWGG 3 cut(s) 522, 791, 876
AluBI AGCT 2 cut(s) 55, 300
AluI AGCT 2 cut(s) 55, 300
Alw21I GWGCWC 2 cut(s) 219, 246
Alw26I GTCTC 3 cut(s) 352, 461, 819
AlwI GGATC 1 cut(s) 744
Ama87I CYCGRG 1 cut(s) 762
AoxI GGCC 8 cut(s) 35, 165, 408, 427, 536, 572, 579, 789
ApeKI GCWGC 1 cut(s) 52
AseI ATTAAT 1 cut(s) 149
AspLEI GCGC 2 cut(s) 381, 383
AspS9I GGNCC 5 cut(s) 166, 579, 721, 755, 789
AsuC2I CCSGG 2 cut(s) 763, 764
AsuHPI GGTGA 1 cut(s) 342
AvaI CYCGRG 1 cut(s) 762
AvaII GGWCC 2 cut(s) 721, 755
BaeI ACNNNNGTAYC 1 cut(s) 32
BalI TGGCCA 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 625
Bbv12I GWGCWC 2 cut(s) 219, 246
BbvI GCAGC 1 cut(s) 64
BceAI ACGGC 2 cut(s) 434, 674
BciT130I CCWGG 3 cut(s) 524, 793, 878
BcnI CCSGG 2 cut(s) 763, 764
BcoDI GTCTC 3 cut(s) 352, 461, 819
BfaI CTAG 3 cut(s) 639, 716, 902
BfmI CTRYAG 1 cut(s) 295
BisI GCNGC 3 cut(s) 53, 408, 582
BlpI GCTNAGC 2 cut(s) 21, 56
BlsI GCNGC 3 cut(s) 54, 409, 583
BmcAI AGTACT 2 cut(s) 467, 667
Bme1390I CCNGG 5 cut(s) 524, 763, 764, 793, 878
Bme18I GGWCC 2 cut(s) 721, 755
BmeT110I CYCGRG 1 cut(s) 762
BmgT120I GGNCC 5 cut(s) 166, 579, 721, 755, 789
BmiI GGNNCC 2 cut(s) 562, 834
BmrFI CCNGG 5 cut(s) 524, 763, 764, 793, 878
BmrI ACTGGG 2 cut(s) 362, 491
BmsI GCATC 2 cut(s) 898, 904
BmuI ACTGGG 2 cut(s) 362, 491
BpiI GAAGAC 1 cut(s) 625
BpmI CTGGAG 1 cut(s) 814
Bpu1102I GCTNAGC 2 cut(s) 21, 56
BpuMI CCSGG 2 cut(s) 763, 764
BsaHI GRCGYC 2 cut(s) 331, 873
BsaJI CCNNGG 8 cut(s) 38, 314, 353, 416, 514, 564, 575, 762
BsaWI WCCGGW 2 cut(s) 557, 752
Bsc4I CCNNNNNNNGG 7 cut(s) 238, 396, 417, 502, 570, 769, 842
Bse118I RCCGGY 2 cut(s) 429, 835
Bse1I ACTGG 3 cut(s) 368, 497, 704
BseBI CCWGG 3 cut(s) 524, 793, 878
BseDI CCNNGG 8 cut(s) 38, 314, 353, 416, 514, 564, 575, 762
BseLI CCNNNNNNNGG 7 cut(s) 238, 396, 417, 502, 570, 769, 842
BseMII CTCAG 3 cut(s) 35, 453, 835
BseNI ACTGG 3 cut(s) 368, 497, 704
BsePI GCGCGC 1 cut(s) 379
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 1 cut(s) 64
Bsh1236I CGCG 1 cut(s) 381
BshFI GGCC 8 cut(s) 37, 167, 410, 429, 538, 574, 581, 791
BsiHKAI GWGCWC 2 cut(s) 219, 246
BsiHKCI CYCGRG 1 cut(s) 762
BsiSI CCGG 8 cut(s) 376, 430, 503, 535, 558, 753, 763, 836
BslFI GGGAC 3 cut(s) 745, 750, 858
BslI CCNNNNNNNGG 7 cut(s) 238, 396, 417, 502, 570, 769, 842
BsmAI GTCTC 3 cut(s) 352, 461, 819
BsmBI CGTCTC 1 cut(s) 819
BsmFI GGGAC 3 cut(s) 745, 750, 858
BsnI GGCC 8 cut(s) 37, 167, 410, 429, 538, 574, 581, 791
BsoBI CYCGRG 1 cut(s) 762
Bsp1286I GDGCHC 2 cut(s) 219, 246
Bsp143I GATC 1 cut(s) 749
Bsp1720I GCTNAGC 2 cut(s) 21, 56
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 6 cut(s) 386, 407, 506, 582, 729, 887
BspANI GGCC 8 cut(s) 37, 167, 410, 429, 538, 574, 581, 791
BspCNI CTCAG 3 cut(s) 34, 454, 834
BspFNI CGCG 1 cut(s) 381
BspLI GGNNCC 2 cut(s) 562, 834
BspPI GGATC 1 cut(s) 744
BsrFI RCCGGY 2 cut(s) 429, 835
BsrI ACTGG 3 cut(s) 368, 497, 704
BssAI RCCGGY 2 cut(s) 429, 835
BssECI CCNNGG 8 cut(s) 38, 314, 353, 416, 514, 564, 575, 762
BssHII GCGCGC 1 cut(s) 379
BssMI GATC 1 cut(s) 749
BssNI GRCGYC 2 cut(s) 331, 873
BssT1I CCWWGG 3 cut(s) 38, 564, 575
Bst2UI CCWGG 3 cut(s) 524, 793, 878
Bst4CI ACNGT 2 cut(s) 772, 815
Bst6I CTCTTC 2 cut(s) 193, 200
BstACI GRCGYC 2 cut(s) 331, 873
BstC8I GCNNGC 4 cut(s) 381, 431, 435, 837
BstDEI CTNAG 6 cut(s) 21, 56, 62, 462, 821, 848
BstDSI CCRYGG 4 cut(s) 38, 314, 416, 514
BstFNI CGCG 1 cut(s) 381
BstHHI GCGC 2 cut(s) 381, 383
BstKTI GATC 1 cut(s) 752
BstMAI GTCTC 3 cut(s) 352, 461, 819
BstMBI GATC 1 cut(s) 749
BstMWI GCNNNNNNNGC 1 cut(s) 385
BstNI CCWGG 3 cut(s) 524, 793, 878
BstSCI CCNGG 5 cut(s) 522, 761, 762, 791, 876
BstSFI CTRYAG 1 cut(s) 295
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 1 cut(s) 64
BstV2I GAAGAC 1 cut(s) 625
BstX2I RGATCY 1 cut(s) 749
BstYI RGATCY 1 cut(s) 749
BsuRI GGCC 8 cut(s) 37, 167, 410, 429, 538, 574, 581, 791
BtgI CCRYGG 4 cut(s) 38, 314, 416, 514
BtsIMutI CAGTG 1 cut(s) 697
Cac8I GCNNGC 4 cut(s) 381, 431, 435, 837
CfoI GCGC 2 cut(s) 381, 383
Cfr10I RCCGGY 2 cut(s) 429, 835
Cfr13I GGNCC 5 cut(s) 166, 579, 721, 755, 789
Cfr9I CCCGGG 1 cut(s) 762
CpoI CGGWCCG 1 cut(s) 755
Csp6I GTAC 3 cut(s) 466, 666, 708
CspCI CAANNNNNGTGG 2 cut(s) 14, 49
CspI CGGWCCG 1 cut(s) 755
CviAII CATG 3 cut(s) 39, 284, 340
CviQI GTAC 3 cut(s) 466, 666, 708
DdeI CTNAG 6 cut(s) 21, 56, 62, 462, 821, 848
DpnI GATC 1 cut(s) 751
DpnII GATC 1 cut(s) 749
EaeI YGGCCR 4 cut(s) 35, 408, 427, 536
Eam1104I CTCTTC 2 cut(s) 193, 200
EarI CTCTTC 2 cut(s) 193, 200
EciI GGCGGA 1 cut(s) 521
Eco130I CCWWGG 3 cut(s) 38, 564, 575
Eco147I AGGCCT 1 cut(s) 574
Eco47I GGWCC 2 cut(s) 721, 755
Eco57I CTGAAG 1 cut(s) 690
Eco88I CYCGRG 1 cut(s) 762
EcoO109I RGGNCCY 1 cut(s) 789
EcoRII CCWGG 3 cut(s) 522, 791, 876
EcoT14I CCWWGG 3 cut(s) 38, 564, 575
ErhI CCWWGG 3 cut(s) 38, 564, 575
Esp3I CGTCTC 1 cut(s) 819
FaeI CATG 3 cut(s) 42, 287, 343
FaiI YATR 9 cut(s) 40, 285, 297, 341, 687, 689, 693, 786, 862
FaqI GGGAC 3 cut(s) 745, 750, 858
FatI CATG 3 cut(s) 38, 283, 339
Fnu4HI GCNGC 3 cut(s) 53, 408, 582
Fsp4HI GCNGC 3 cut(s) 53, 408, 582
FspBI CTAG 3 cut(s) 639, 716, 902
GlaI GCGC 2 cut(s) 380, 382
GluI GCNGC 3 cut(s) 53, 408, 582
GsuI CTGGAG 1 cut(s) 814
HaeIII GGCC 8 cut(s) 37, 167, 410, 429, 538, 574, 581, 791
HapII CCGG 8 cut(s) 376, 430, 503, 535, 558, 753, 763, 836
HhaI GCGC 2 cut(s) 381, 383
Hin1I GRCGYC 2 cut(s) 331, 873
Hin1II CATG 3 cut(s) 42, 287, 343
Hin6I GCGC 2 cut(s) 379, 381
HinP1I GCGC 2 cut(s) 379, 381
HinfI GANTC 2 cut(s) 488, 852
HpaII CCGG 8 cut(s) 376, 430, 503, 535, 558, 753, 763, 836
HphI GGTGA 1 cut(s) 342
Hpy166II GTNNAC 1 cut(s) 697
Hpy188I TCNGA 6 cut(s) 255, 357, 546, 600, 811, 824
Hpy188III TCNNGA 1 cut(s) 869
Hpy8I GTNNAC 1 cut(s) 697
Hpy99I CGWCG 3 cut(s) 660, 772, 875
HpyCH4III ACNGT 2 cut(s) 772, 815
HpyCH4IV ACGT 5 cut(s) 132, 331, 594, 739, 873
HpyCH4V TGCA 2 cut(s) 4, 45
HpyF10VI GCNNNNNNNGC 1 cut(s) 385
HpyF3I CTNAG 6 cut(s) 21, 56, 62, 462, 821, 848
HpySE526I ACGT 5 cut(s) 132, 331, 594, 739, 873
Hsp92I GRCGYC 2 cut(s) 331, 873
Hsp92II CATG 3 cut(s) 42, 287, 343
HspAI GCGC 2 cut(s) 379, 381
KroI GCCGGC 2 cut(s) 429, 835
KroNI GCCGGC 2 cut(s) 431, 837
Kzo9I GATC 1 cut(s) 749
LmnI GCTCC 1 cut(s) 832
Lsp1109I GCAGC 1 cut(s) 64
LweI GCATC 2 cut(s) 898, 904
MaeI CTAG 3 cut(s) 639, 716, 902
MaeII ACGT 5 cut(s) 132, 331, 594, 739, 873
MalI GATC 1 cut(s) 751
MboI GATC 1 cut(s) 749
MboII GAAGA 7 cut(s) 175, 180, 187, 198, 355, 625, 890
MflI RGATCY 1 cut(s) 749
MhlI GDGCHC 2 cut(s) 219, 246
MlsI TGGCCA 1 cut(s) 37
MluCI AATT 3 cut(s) 98, 401, 586
MluNI TGGCCA 1 cut(s) 37
MmeI TCCRAC 2 cut(s) 278, 569
Mox20I TGGCCA 1 cut(s) 37
MroNI GCCGGC 2 cut(s) 429, 835
MscI TGGCCA 1 cut(s) 37
MseI TTAA 2 cut(s) 84, 149
MslI CAYNNNNRTG 1 cut(s) 282
Msp20I TGGCCA 1 cut(s) 37
MspI CCGG 8 cut(s) 376, 430, 503, 535, 558, 753, 763, 836
MspR9I CCNGG 5 cut(s) 524, 763, 764, 793, 878
MvaI CCWGG 3 cut(s) 524, 793, 878
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 1 cut(s) 385
NaeI GCCGGC 2 cut(s) 431, 837
NciI CCSGG 2 cut(s) 763, 764
NcoI CCATGG 1 cut(s) 38
NdeII GATC 1 cut(s) 749
NgoMIV GCCGGC 2 cut(s) 429, 835
NlaIII CATG 3 cut(s) 42, 287, 343
NlaIV GGNNCC 2 cut(s) 562, 834
PauI GCGCGC 1 cut(s) 379
PceI AGGCCT 1 cut(s) 574
PcsI WCGNNNNNNNCGW 1 cut(s) 642
PdiI GCCGGC 2 cut(s) 431, 837
PfeI GAWTC 2 cut(s) 488, 852
PkrI GCNGC 3 cut(s) 54, 409, 583
PshBI ATTAAT 1 cut(s) 149
Psp1406I AACGTT 1 cut(s) 132
Psp6I CCWGG 3 cut(s) 522, 791, 876
PspGI CCWGG 3 cut(s) 522, 791, 876
PspN4I GGNNCC 2 cut(s) 562, 834
PspPI GGNCC 5 cut(s) 166, 579, 721, 755, 789
PsuI RGATCY 1 cut(s) 749
PteI GCGCGC 1 cut(s) 379
RsaI GTAC 3 cut(s) 467, 667, 709
RsaNI GTAC 3 cut(s) 466, 666, 708
RseI CAYNNNNRTG 1 cut(s) 282
Rsr2I CGGWCCG 1 cut(s) 755
RsrII CGGWCCG 1 cut(s) 755
SaqAI TTAA 2 cut(s) 84, 149
SatI GCNGC 3 cut(s) 53, 408, 582
Sau3AI GATC 1 cut(s) 749
Sau96I GGNCC 5 cut(s) 166, 579, 721, 755, 789
ScaI AGTACT 2 cut(s) 467, 667
ScrFI CCNGG 5 cut(s) 524, 763, 764, 793, 878
SduI GDGCHC 2 cut(s) 219, 246
SfaNI GCATC 2 cut(s) 898, 904
SfcI CTRYAG 1 cut(s) 295
SinI GGWCC 2 cut(s) 721, 755
SmaI CCCGGG 1 cut(s) 764
SmiMI CAYNNNNRTG 1 cut(s) 282
Sse9I AATT 3 cut(s) 98, 401, 586
SseBI AGGCCT 1 cut(s) 574
SsiI CCGC 6 cut(s) 386, 407, 506, 582, 729, 887
SspMI CTAG 3 cut(s) 639, 716, 902
StuI AGGCCT 1 cut(s) 574
StyD4I CCNGG 5 cut(s) 522, 761, 762, 791, 876
StyI CCWWGG 3 cut(s) 38, 564, 575
TaaI ACNGT 2 cut(s) 772, 815
TaiI ACGT 5 cut(s) 135, 334, 597, 742, 876
TaqI TCGA 4 cut(s) 395, 611, 655, 855
TasI AATT 3 cut(s) 98, 401, 586
TatI WGTACW 3 cut(s) 465, 665, 707
TauI GCSGC 2 cut(s) 410, 584
TfiI GAWTC 2 cut(s) 488, 852
Tru1I TTAA 2 cut(s) 84, 149
Tru9I TTAA 2 cut(s) 84, 149
TscAI CASTG 1 cut(s) 704
TseI GCWGC 1 cut(s) 52
TspDTI ATGAA 2 cut(s) 135, 356
TspGWI ACGGA 3 cut(s) 202, 619, 747
TspMI CCCGGG 1 cut(s) 762
TspRI CASTG 1 cut(s) 704
VpaK11BI GGWCC 2 cut(s) 721, 755
VspI ATTAAT 1 cut(s) 149
XmaI CCCGGG 1 cut(s) 762
XspI CTAG 3 cut(s) 639, 716, 902
ZraI GACGTC 2 cut(s) 332, 874
ZrmI AGTACT 2 cut(s) 467, 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.