pycom10g08970

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002872
Physical Location & Seq
Reverse (-)
11856187 .. 11858435
2249 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g08970.1

Sequence Viewer

Length: 708 bp
ATGTGCACAAACTATGTTGGGGTAAGGGAGGTTGCCACTTGCAGAATGTCCTCTGCTAAGTCCCTCCAGCACTATCAACAGCTTAACGTGGAGTGTAGTCTCAATCTCGTCCTTTGTAACAACCCGTCCCGGAATTTACGTAAGAAAATGAGTATTTTTGTATTTTTCACTAATGGGGGGCCCAAGGGGCCCCCCCCCTCTTTCTCTCCCCCCCCCCCCCCCCCGAGCCTCTCACATTTTCCTTCTCTCTCAGCTCTCTTTTCTTTCTTTTCCCCGAACCTCTCTCCCTTACCCATTCTCTTTCTCTTTTCTAATCCCGTGGTTGTGTTTTGGACGTCAAATCACGAAGAAACCACCTCGGAGACATTTCCCAGTTTTCCGGCGAGCACCGCCCCTTTCGAGGAAATTTCCGGCCAAACCACGGCGAGTTGGCCGGCGTGCAAGTTGCCGGCGGACACCCGTGGCTTCCAGGCTGTTTTCCGGCCAACTCCGACCACGATGACGGGAACCAAGGGGGCAATTATCTGTGGCGTTTCCTTCTTCGCTAGATGGTGTGGCTTTTCGACGGCGAAGTACTATCCGGACTTAGAATCGAGGCATACAATCCCGACGTCCAGGCACTTCCGCATCAGCATCTTTGAGTCCTCTCGGTGTAGGACCCACTCCTTGGTTTCATTCAATTTTATATTATTTTCTTTAGTGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

236

Amino Acids

26.14

Weight (kDa)

9.28

Isoelectric Point (pI)

60.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 338, 614
AccB7I CCANNNNNTGG 1 cut(s) 667
AccIII TCCGGA 1 cut(s) 580
AciI CCGC 3 cut(s) 390, 452, 625
AcoI YGGCCR 3 cut(s) 412, 431, 482
AcsI RAATTY 2 cut(s) 133, 405
AcyI GRCGYC 2 cut(s) 335, 611
AfaI GTAC 1 cut(s) 575
AfiI CCNNNNNNNGG 3 cut(s) 400, 421, 667
AgsI TTSAA 1 cut(s) 679
AjnI CCWGG 2 cut(s) 468, 614
AluBI AGCT 2 cut(s) 82, 254
AluI AGCT 2 cut(s) 82, 254
Alw21I GWGCWC 2 cut(s) 8, 389
Alw26I GTCTC 2 cut(s) 104, 356
Alw44I GTGCAC 1 cut(s) 4
Ama87I CYCGRG 1 cut(s) 223
Aor13HI TCCGGA 1 cut(s) 580
AoxI GGCC 5 cut(s) 179, 188, 412, 431, 482
ApaI GGGCCC 2 cut(s) 183, 192
ApaLI GTGCAC 1 cut(s) 4
ApoI RAATTY 2 cut(s) 133, 405
AspS9I GGNCC 5 cut(s) 179, 180, 188, 189, 657
AsuC2I CCSGG 1 cut(s) 130
AvaI CYCGRG 1 cut(s) 223
AvaII GGWCC 1 cut(s) 657
BaeGI GKGCMC 3 cut(s) 8, 183, 192
BanII GRGCYC 2 cut(s) 183, 192
Bbv12I GWGCWC 2 cut(s) 8, 389
BccI CCATC 1 cut(s) 543
BceAI ACGGC 2 cut(s) 438, 582
BciT130I CCWGG 2 cut(s) 470, 616
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 2 cut(s) 104, 356
BfaI CTAG 2 cut(s) 546, 706
BglI GCCNNNNNGGC 1 cut(s) 187
BmcAI AGTACT 1 cut(s) 575
Bme1390I CCNGG 3 cut(s) 130, 470, 616
Bme18I GGWCC 1 cut(s) 657
BmeT110I CYCGRG 1 cut(s) 223
BmgT120I GGNCC 5 cut(s) 179, 180, 188, 189, 657
BmiI GGNNCC 7 cut(s) 180, 181, 189, 190, 191, 508, 659
BmrFI CCNGG 3 cut(s) 130, 470, 616
BmrI ACTGGG 1 cut(s) 366
BmsI GCATC 2 cut(s) 636, 642
BmuI ACTGGG 1 cut(s) 366
BpmI CTGGAG 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 130
BsaAI YACGTR 1 cut(s) 140
BsaHI GRCGYC 2 cut(s) 335, 611
BsaJI CCNNGG 7 cut(s) 183, 318, 357, 420, 460, 510, 666
BsaWI WCCGGW 1 cut(s) 580
Bsc4I CCNNNNNNNGG 3 cut(s) 400, 421, 667
Bse118I RCCGGY 2 cut(s) 433, 448
Bse1I ACTGG 1 cut(s) 372
BseAI TCCGGA 1 cut(s) 580
BseBI CCWGG 2 cut(s) 470, 616
BseDI CCNNGG 7 cut(s) 183, 318, 357, 420, 460, 510, 666
BseLI CCNNNNNNNGG 3 cut(s) 400, 421, 667
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 1 cut(s) 372
BseSI GKGCMC 3 cut(s) 8, 183, 192
BshFI GGCC 5 cut(s) 181, 190, 414, 433, 484
BsiHKAI GWGCWC 2 cut(s) 8, 389
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 7 cut(s) 130, 380, 411, 434, 449, 481, 581
BslFI GGGAC 2 cut(s) 46, 112
BslI CCNNNNNNNGG 3 cut(s) 400, 421, 667
BsmAI GTCTC 2 cut(s) 104, 356
BsmFI GGGAC 2 cut(s) 46, 112
BsnI GGCC 5 cut(s) 181, 190, 414, 433, 484
BsoBI CYCGRG 1 cut(s) 223
Bsp120I GGGCCC 2 cut(s) 179, 188
Bsp1286I GDGCHC 4 cut(s) 8, 183, 192, 389
Bsp13I TCCGGA 1 cut(s) 580
BspACI CCGC 3 cut(s) 390, 452, 625
BspANI GGCC 5 cut(s) 181, 190, 414, 433, 484
BspCNI CTCAG 1 cut(s) 263
BspEI TCCGGA 1 cut(s) 580
BspLI GGNNCC 7 cut(s) 180, 181, 189, 190, 191, 508, 659
BsrFI RCCGGY 2 cut(s) 433, 448
BsrI ACTGG 1 cut(s) 372
BssAI RCCGGY 2 cut(s) 433, 448
BssECI CCNNGG 7 cut(s) 183, 318, 357, 420, 460, 510, 666
BssNI GRCGYC 2 cut(s) 335, 611
BssT1I CCWWGG 3 cut(s) 183, 510, 666
Bst2UI CCWGG 2 cut(s) 470, 616
BstACI GRCGYC 2 cut(s) 335, 611
BstBAI YACGTR 1 cut(s) 140
BstC8I GCNNGC 4 cut(s) 385, 435, 439, 450
BstDEI CTNAG 3 cut(s) 57, 250, 586
BstDSI CCRYGG 3 cut(s) 318, 420, 460
BstMAI GTCTC 2 cut(s) 104, 356
BstMWI GCNNNNNNNGC 2 cut(s) 187, 389
BstNI CCWGG 2 cut(s) 470, 616
BstSCI CCNGG 3 cut(s) 128, 468, 614
BstSLI GKGCMC 3 cut(s) 8, 183, 192
BstSNI TACGTA 1 cut(s) 140
BsuRI GGCC 5 cut(s) 181, 190, 414, 433, 484
BtgI CCRYGG 3 cut(s) 318, 420, 460
Cac8I GCNNGC 4 cut(s) 385, 435, 439, 450
Cfr10I RCCGGY 2 cut(s) 433, 448
Cfr13I GGNCC 5 cut(s) 179, 180, 188, 189, 657
Csp6I GTAC 1 cut(s) 574
CviQI GTAC 1 cut(s) 574
DdeI CTNAG 3 cut(s) 57, 250, 586
EaeI YGGCCR 3 cut(s) 412, 431, 482
EciI GGCGGA 1 cut(s) 467
Eco105I TACGTA 1 cut(s) 140
Eco130I CCWWGG 3 cut(s) 183, 510, 666
Eco24I GRGCYC 2 cut(s) 183, 192
Eco47I GGWCC 1 cut(s) 657
Eco88I CYCGRG 1 cut(s) 223
EcoO109I RGGNCCY 4 cut(s) 179, 188, 189, 657
EcoRII CCWGG 2 cut(s) 468, 614
EcoT14I CCWWGG 3 cut(s) 183, 510, 666
EcoT38I GRGCYC 2 cut(s) 183, 192
ErhI CCWWGG 3 cut(s) 183, 510, 666
FaiI YATR 3 cut(s) 15, 600, 686
FaqI GGGAC 2 cut(s) 46, 112
FriOI GRGCYC 2 cut(s) 183, 192
FspBI CTAG 2 cut(s) 546, 706
GsuI CTGGAG 1 cut(s) 50
HaeIII GGCC 5 cut(s) 181, 190, 414, 433, 484
HapII CCGG 7 cut(s) 130, 380, 411, 434, 449, 481, 581
Hin1I GRCGYC 2 cut(s) 335, 611
HinfI GANTC 2 cut(s) 590, 641
HpaII CCGG 7 cut(s) 130, 380, 411, 434, 449, 481, 581
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 361, 492
Hpy188III TCNNGA 3 cut(s) 344, 581, 607
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 2 cut(s) 568, 613
HpyAV CCTTC 2 cut(s) 252, 547
HpyCH4IV ACGT 4 cut(s) 87, 139, 335, 611
HpyCH4V TGCA 3 cut(s) 6, 42, 441
HpyF10VI GCNNNNNNNGC 2 cut(s) 187, 389
HpyF3I CTNAG 3 cut(s) 57, 250, 586
HpySE526I ACGT 4 cut(s) 87, 139, 335, 611
Hsp92I GRCGYC 2 cut(s) 335, 611
Kpn2I TCCGGA 1 cut(s) 580
KroI GCCGGC 2 cut(s) 433, 448
KroNI GCCGGC 2 cut(s) 435, 450
LweI GCATC 2 cut(s) 636, 642
MaeI CTAG 2 cut(s) 546, 706
MaeII ACGT 4 cut(s) 87, 139, 335, 611
MaeIII GTNAC 1 cut(s) 116
MboII GAAGA 2 cut(s) 359, 532
MhlI GDGCHC 4 cut(s) 8, 183, 192, 389
MluCI AATT 4 cut(s) 133, 405, 519, 679
MlyI GAGTC 1 cut(s) 650
MmeI TCCRAC 1 cut(s) 515
MroI TCCGGA 1 cut(s) 580
MroNI GCCGGC 2 cut(s) 433, 448
MseI TTAA 1 cut(s) 84
MspI CCGG 7 cut(s) 130, 380, 411, 434, 449, 481, 581
MspR9I CCNGG 3 cut(s) 130, 470, 616
MvaI CCWGG 2 cut(s) 470, 616
MwoI GCNNNNNNNGC 2 cut(s) 187, 389
NaeI GCCGGC 2 cut(s) 435, 450
NciI CCSGG 1 cut(s) 130
NgoMIV GCCGGC 2 cut(s) 433, 448
NlaIV GGNNCC 7 cut(s) 180, 181, 189, 190, 191, 508, 659
PdiI GCCGGC 2 cut(s) 435, 450
PfeI GAWTC 1 cut(s) 590
PflMI CCANNNNNTGG 1 cut(s) 667
PfoI TCCNGGA 1 cut(s) 128
PleI GAGTC 1 cut(s) 649
PpsI GAGTC 1 cut(s) 649
Ppu21I YACGTR 1 cut(s) 140
PpuMI RGGWCCY 1 cut(s) 657
Psp5II RGGWCCY 1 cut(s) 657
Psp6I CCWGG 2 cut(s) 468, 614
PspGI CCWGG 2 cut(s) 468, 614
PspN4I GGNNCC 7 cut(s) 180, 181, 189, 190, 191, 508, 659
PspOMI GGGCCC 2 cut(s) 179, 188
PspPI GGNCC 5 cut(s) 179, 180, 188, 189, 657
PspPPI RGGWCCY 1 cut(s) 657
RsaI GTAC 1 cut(s) 575
RsaNI GTAC 1 cut(s) 574
SaqAI TTAA 1 cut(s) 84
Sau96I GGNCC 5 cut(s) 179, 180, 188, 189, 657
ScaI AGTACT 1 cut(s) 575
SchI GAGTC 1 cut(s) 650
ScrFI CCNGG 3 cut(s) 130, 470, 616
SduI GDGCHC 4 cut(s) 8, 183, 192, 389
SetI ASST 9 cut(s) 33, 84, 90, 142, 256, 282, 338, 359, 614
SfaNI GCATC 2 cut(s) 636, 642
SfiI GGCCNNNNNGGCC 1 cut(s) 187
SinI GGWCC 1 cut(s) 657
SnaBI TACGTA 1 cut(s) 140
Sse9I AATT 4 cut(s) 133, 405, 519, 679
SsiI CCGC 3 cut(s) 390, 452, 625
SspMI CTAG 2 cut(s) 546, 706
StyD4I CCNGG 3 cut(s) 128, 468, 614
StyI CCWWGG 3 cut(s) 183, 510, 666
TaiI ACGT 4 cut(s) 90, 142, 338, 614
TaqI TCGA 3 cut(s) 399, 563, 593
TasI AATT 4 cut(s) 133, 405, 519, 679
TatI WGTACW 1 cut(s) 573
TfiI GAWTC 1 cut(s) 590
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TspDTI ATGAA 1 cut(s) 663
Van91I CCANNNNNTGG 1 cut(s) 667
VneI GTGCAC 1 cut(s) 4
VpaK11BI GGWCC 1 cut(s) 657
XapI RAATTY 2 cut(s) 133, 405
XspI CTAG 2 cut(s) 546, 706
ZraI GACGTC 2 cut(s) 336, 612
ZrmI AGTACT 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.