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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 143 |
| AciI | CCGC | 1 cut(s) | 154 |
| AcyI | GRCGYC | 1 cut(s) | 140 |
| AfaI | GTAC | 1 cut(s) | 109 |
| AgsI | TTSAA | 1 cut(s) | 208 |
| AjnI | CCWGG | 1 cut(s) | 143 |
| AspS9I | GGNCC | 1 cut(s) | 186 |
| AvaII | GGWCC | 1 cut(s) | 186 |
| BciT130I | CCWGG | 1 cut(s) | 145 |
| BfaI | CTAG | 3 cut(s) | 48, 81, 236 |
| BmcAI | AGTACT | 1 cut(s) | 109 |
| Bme1390I | CCNGG | 1 cut(s) | 145 |
| Bme18I | GGWCC | 1 cut(s) | 186 |
| BmgT120I | GGNCC | 1 cut(s) | 186 |
| BmiI | GGNNCC | 2 cut(s) | 10, 188 |
| BmrFI | CCNGG | 1 cut(s) | 145 |
| BmsI | GCATC | 3 cut(s) | 63, 165, 171 |
| BsaHI | GRCGYC | 1 cut(s) | 140 |
| BsaJI | CCNNGG | 1 cut(s) | 12 |
| BseBI | CCWGG | 1 cut(s) | 145 |
| BseDI | CCNNGG | 1 cut(s) | 12 |
| BseGI | GGATG | 1 cut(s) | 54 |
| BspACI | CCGC | 1 cut(s) | 154 |
| BspLI | GGNNCC | 2 cut(s) | 10, 188 |
| BssECI | CCNNGG | 1 cut(s) | 12 |
| BssNI | GRCGYC | 1 cut(s) | 140 |
| BssT1I | CCWWGG | 1 cut(s) | 12 |
| Bst2UI | CCWGG | 1 cut(s) | 145 |
| BstACI | GRCGYC | 1 cut(s) | 140 |
| BstDEI | CTNAG | 1 cut(s) | 115 |
| BstF5I | GGATG | 1 cut(s) | 54 |
| BstNI | CCWGG | 1 cut(s) | 145 |
| BstSCI | CCNGG | 1 cut(s) | 143 |
| BtsCI | GGATG | 1 cut(s) | 54 |
| Cfr13I | GGNCC | 1 cut(s) | 186 |
| Csp6I | GTAC | 1 cut(s) | 108 |
| CviJI | RGCY | 1 cut(s) | 92 |
| CviKI_1 | RGCY | 1 cut(s) | 92 |
| CviQI | GTAC | 1 cut(s) | 108 |
| DdeI | CTNAG | 1 cut(s) | 115 |
| Eco130I | CCWWGG | 1 cut(s) | 12 |
| Eco47I | GGWCC | 1 cut(s) | 186 |
| EcoO109I | RGGNCCY | 1 cut(s) | 186 |
| EcoRII | CCWGG | 1 cut(s) | 143 |
| EcoT14I | CCWWGG | 1 cut(s) | 12 |
| ErhI | CCWWGG | 1 cut(s) | 12 |
| FaiI | YATR | 2 cut(s) | 129, 215 |
| FokI | GGATG | 1 cut(s) | 41 |
| FspBI | CTAG | 3 cut(s) | 48, 81, 236 |
| Hin1I | GRCGYC | 1 cut(s) | 140 |
| HinfI | GANTC | 1 cut(s) | 119 |
| Hpy188III | TCNNGA | 2 cut(s) | 136, 236 |
| Hpy99I | CGWCG | 1 cut(s) | 142 |
| HpyCH4IV | ACGT | 1 cut(s) | 140 |
| HpyF3I | CTNAG | 1 cut(s) | 115 |
| HpySE526I | ACGT | 1 cut(s) | 140 |
| Hsp92I | GRCGYC | 1 cut(s) | 140 |
| LpnPI | CCDG | 2 cut(s) | 130, 157 |
| LweI | GCATC | 3 cut(s) | 63, 165, 171 |
| MaeI | CTAG | 3 cut(s) | 48, 81, 236 |
| MaeII | ACGT | 1 cut(s) | 140 |
| MboII | GAAGA | 1 cut(s) | 157 |
| MluCI | AATT | 1 cut(s) | 208 |
| MnlI | CCTC | 1 cut(s) | 117 |
| MspR9I | CCNGG | 1 cut(s) | 145 |
| MvaI | CCWGG | 1 cut(s) | 145 |
| NlaIV | GGNNCC | 2 cut(s) | 10, 188 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 84, 165 |
| PfeI | GAWTC | 1 cut(s) | 119 |
| PpuMI | RGGWCCY | 1 cut(s) | 186 |
| Psp5II | RGGWCCY | 1 cut(s) | 186 |
| Psp6I | CCWGG | 1 cut(s) | 143 |
| PspGI | CCWGG | 1 cut(s) | 143 |
| PspN4I | GGNNCC | 2 cut(s) | 10, 188 |
| PspPI | GGNCC | 1 cut(s) | 186 |
| PspPPI | RGGWCCY | 1 cut(s) | 186 |
| RsaI | GTAC | 1 cut(s) | 109 |
| RsaNI | GTAC | 1 cut(s) | 108 |
| Sau96I | GGNCC | 1 cut(s) | 186 |
| ScaI | AGTACT | 1 cut(s) | 109 |
| ScrFI | CCNGG | 1 cut(s) | 145 |
| SetI | ASST | 1 cut(s) | 143 |
| SfaNI | GCATC | 3 cut(s) | 63, 165, 171 |
| SinI | GGWCC | 1 cut(s) | 186 |
| Sse9I | AATT | 1 cut(s) | 208 |
| SsiI | CCGC | 1 cut(s) | 154 |
| SspMI | CTAG | 3 cut(s) | 48, 81, 236 |
| StyD4I | CCNGG | 1 cut(s) | 143 |
| StyI | CCWWGG | 1 cut(s) | 12 |
| TaiI | ACGT | 1 cut(s) | 143 |
| TaqI | TCGA | 3 cut(s) | 97, 122, 168 |
| TasI | AATT | 1 cut(s) | 208 |
| TatI | WGTACW | 1 cut(s) | 107 |
| TfiI | GAWTC | 1 cut(s) | 119 |
| TspDTI | ATGAA | 1 cut(s) | 192 |
| TspGWI | ACGGA | 3 cut(s) | 28, 47, 61 |
| VpaK11BI | GGWCC | 1 cut(s) | 186 |
| XbaI | TCTAGA | 1 cut(s) | 235 |
| XspI | CTAG | 3 cut(s) | 48, 81, 236 |
| ZraI | GACGTC | 1 cut(s) | 141 |
| ZrmI | AGTACT | 1 cut(s) | 109 |