pycom12g00190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002872
Physical Location & Seq
Reverse (-)
265863 .. 267987
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g00190.3

Sequence Viewer

Length: 576 bp
ATGGGTATTTTTGTATTTTCATTAACCCAAGGGGCCCACCCCCTGTTTTGTCCCCCGGTTCCCTCTCCCTTTTCTTTTTTTTCTGAAAAGTCTTCTCTTCTCTCTTCTCTCTTTCTTTCTCCGTGCTCTCTCTTTCCCCCCACCTCTCGGTGCTCTTTTCTCCTACTCTTTTCTAACCCTGTGGTTGTGTTTTGGACGTCAAACCACGAAGAAACCACCTCGGAGACATTTCCCAGTTTTCCGGCGAGCATCGCTCCTTTCGAGGCAATTTCCGGCCAAACCACGGCGAGTTGGCCGGCGTGCAAGTTTCCGGCGGACACCCGTGGCTTCCAGGCTGTTTTCCGGCCAACTCCGGCCACGATGACGGGAACCAAGGGGGCGATTATCTGTGGCGTTTCCTTCTTCGCTAAGTGGCGTGGCTTTTCGACGGCGAAGTACTATCCGGATTTAGAATCGAGGCATACAATCCCGACGTCCAGACACTTCCGCATCGGCATCTTTGAGTCCTCTCGGTGTAGGACCCACTCCTTTGTTTCATTCAATTTTACATTATTTTCTTTTAGTGTTCTAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

21.19

Weight (kDa)

8.86

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 200, 476
AccIII TCCGGA 1 cut(s) 442
AciI CCGC 2 cut(s) 314, 487
AcoI YGGCCR 4 cut(s) 274, 293, 344, 354
AcyI GRCGYC 2 cut(s) 197, 473
AfaI GTAC 1 cut(s) 437
AfiI CCNNNNNNNGG 2 cut(s) 147, 283
AgsI TTSAA 1 cut(s) 541
AjnI CCWGG 1 cut(s) 330
Alw21I GWGCWC 2 cut(s) 128, 155
Alw26I GTCTC 1 cut(s) 218
Aor13HI TCCGGA 1 cut(s) 442
AoxI GGCC 5 cut(s) 33, 274, 293, 344, 354
ApaI GGGCCC 1 cut(s) 37
AspS9I GGNCC 3 cut(s) 33, 34, 519
AsuC2I CCSGG 1 cut(s) 56
AvaII GGWCC 1 cut(s) 519
BaeGI GKGCMC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 2 cut(s) 128, 155
BceAI ACGGC 2 cut(s) 300, 444
BciT130I CCWGG 1 cut(s) 332
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 1 cut(s) 218
BfaI CTAG 1 cut(s) 569
BmcAI AGTACT 1 cut(s) 437
Bme1390I CCNGG 2 cut(s) 56, 332
Bme18I GGWCC 1 cut(s) 519
BmgT120I GGNCC 3 cut(s) 33, 34, 519
BmiI GGNNCC 5 cut(s) 34, 35, 60, 370, 521
BmrFI CCNGG 2 cut(s) 56, 332
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 3 cut(s) 258, 498, 504
BmuI ACTGGG 1 cut(s) 228
BpiI GAAGAC 1 cut(s) 84
BplI GAGNNNNNCTC 2 cut(s) 238, 270
BpuMI CCSGG 1 cut(s) 56
BsaHI GRCGYC 2 cut(s) 197, 473
BsaJI CCNNGG 6 cut(s) 28, 54, 219, 282, 322, 372
BsaWI WCCGGW 1 cut(s) 442
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 283
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 1 cut(s) 234
BseAI TCCGGA 1 cut(s) 442
BseBI CCWGG 1 cut(s) 332
BseDI CCNNGG 6 cut(s) 28, 54, 219, 282, 322, 372
BseLI CCNNNNNNNGG 2 cut(s) 147, 283
BseNI ACTGG 1 cut(s) 234
BseSI GKGCMC 1 cut(s) 37
BshFI GGCC 5 cut(s) 35, 276, 295, 346, 356
BsiHKAI GWGCWC 2 cut(s) 128, 155
BsiSI CCGG 8 cut(s) 56, 242, 273, 296, 311, 343, 353, 443
BslFI GGGAC 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 147, 283
BsmAI GTCTC 1 cut(s) 218
BsmFI GGGAC 1 cut(s) 36
BsnI GGCC 5 cut(s) 35, 276, 295, 346, 356
Bsp120I GGGCCC 1 cut(s) 33
Bsp1286I GDGCHC 3 cut(s) 37, 128, 155
Bsp13I TCCGGA 1 cut(s) 442
BspACI CCGC 2 cut(s) 314, 487
BspANI GGCC 5 cut(s) 35, 276, 295, 346, 356
BspEI TCCGGA 1 cut(s) 442
BspLI GGNNCC 5 cut(s) 34, 35, 60, 370, 521
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 1 cut(s) 234
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 6 cut(s) 28, 54, 219, 282, 322, 372
BssNI GRCGYC 2 cut(s) 197, 473
BssT1I CCWWGG 2 cut(s) 28, 372
Bst2UI CCWGG 1 cut(s) 332
Bst6I CTCTTC 2 cut(s) 102, 109
BstACI GRCGYC 2 cut(s) 197, 473
BstC8I GCNNGC 3 cut(s) 247, 297, 301
BstDEI CTNAG 1 cut(s) 408
BstDSI CCRYGG 2 cut(s) 282, 322
BstMAI GTCTC 1 cut(s) 218
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNI CCWGG 1 cut(s) 332
BstSCI CCNGG 2 cut(s) 54, 330
BstSLI GKGCMC 1 cut(s) 37
BstV2I GAAGAC 1 cut(s) 84
BsuRI GGCC 5 cut(s) 35, 276, 295, 346, 356
BtgI CCRYGG 2 cut(s) 282, 322
BtgZI GCGATG 1 cut(s) 235
Cac8I GCNNGC 3 cut(s) 247, 297, 301
Cfr10I RCCGGY 1 cut(s) 295
Cfr13I GGNCC 3 cut(s) 33, 34, 519
Csp6I GTAC 1 cut(s) 436
CviJI RGCY 8 cut(s) 35, 276, 295, 327, 335, 346, 356, 420
CviKI_1 RGCY 8 cut(s) 35, 276, 295, 327, 335, 346, 356, 420
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 1 cut(s) 408
EaeI YGGCCR 4 cut(s) 274, 293, 344, 354
Eam1104I CTCTTC 2 cut(s) 102, 109
EarI CTCTTC 2 cut(s) 102, 109
EciI GGCGGA 1 cut(s) 329
Eco130I CCWWGG 2 cut(s) 28, 372
Eco24I GRGCYC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 519
EcoO109I RGGNCCY 2 cut(s) 33, 519
EcoRII CCWGG 1 cut(s) 330
EcoT14I CCWWGG 2 cut(s) 28, 372
EcoT38I GRGCYC 1 cut(s) 37
ErhI CCWWGG 2 cut(s) 28, 372
FaiI YATR 1 cut(s) 462
FaqI GGGAC 1 cut(s) 36
FriOI GRGCYC 1 cut(s) 37
FspBI CTAG 1 cut(s) 569
HaeIII GGCC 5 cut(s) 35, 276, 295, 346, 356
HapII CCGG 8 cut(s) 56, 242, 273, 296, 311, 343, 353, 443
Hin1I GRCGYC 2 cut(s) 197, 473
HinfI GANTC 2 cut(s) 452, 503
HpaII CCGG 8 cut(s) 56, 242, 273, 296, 311, 343, 353, 443
Hpy188I TCNGA 2 cut(s) 85, 223
Hpy188III TCNNGA 4 cut(s) 443, 469, 477, 569
Hpy99I CGWCG 2 cut(s) 430, 475
HpyAV CCTTC 1 cut(s) 409
HpyCH4IV ACGT 2 cut(s) 197, 473
HpyCH4V TGCA 1 cut(s) 303
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 1 cut(s) 408
HpySE526I ACGT 2 cut(s) 197, 473
Hsp92I GRCGYC 2 cut(s) 197, 473
Kpn2I TCCGGA 1 cut(s) 442
KroI GCCGGC 1 cut(s) 295
KroNI GCCGGC 1 cut(s) 297
LmnI GCTCC 1 cut(s) 259
LweI GCATC 3 cut(s) 258, 498, 504
MaeI CTAG 1 cut(s) 569
MaeII ACGT 2 cut(s) 197, 473
MboII GAAGA 5 cut(s) 84, 89, 96, 221, 394
MhlI GDGCHC 3 cut(s) 37, 128, 155
MluCI AATT 2 cut(s) 267, 541
MlyI GAGTC 1 cut(s) 512
MnlI CCTC 6 cut(s) 73, 154, 229, 256, 450, 517
MroI TCCGGA 1 cut(s) 442
MroNI GCCGGC 1 cut(s) 295
MseI TTAA 1 cut(s) 23
MspI CCGG 8 cut(s) 56, 242, 273, 296, 311, 343, 353, 443
MspR9I CCNGG 2 cut(s) 56, 332
MvaI CCWGG 1 cut(s) 332
MwoI GCNNNNNNNGC 1 cut(s) 251
NaeI GCCGGC 1 cut(s) 297
NciI CCSGG 1 cut(s) 56
NgoMIV GCCGGC 1 cut(s) 295
NlaIV GGNNCC 5 cut(s) 34, 35, 60, 370, 521
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PdiI GCCGGC 1 cut(s) 297
PfeI GAWTC 1 cut(s) 452
PleI GAGTC 1 cut(s) 511
PpsI GAGTC 1 cut(s) 511
PpuMI RGGWCCY 1 cut(s) 519
Psp5II RGGWCCY 1 cut(s) 519
Psp6I CCWGG 1 cut(s) 330
PspGI CCWGG 1 cut(s) 330
PspN4I GGNNCC 5 cut(s) 34, 35, 60, 370, 521
PspOMI GGGCCC 1 cut(s) 33
PspPI GGNCC 3 cut(s) 33, 34, 519
PspPPI RGGWCCY 1 cut(s) 519
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
SaqAI TTAA 1 cut(s) 23
Sau96I GGNCC 3 cut(s) 33, 34, 519
ScaI AGTACT 1 cut(s) 437
SchI GAGTC 1 cut(s) 512
ScrFI CCNGG 2 cut(s) 56, 332
SduI GDGCHC 3 cut(s) 37, 128, 155
SetI ASST 4 cut(s) 146, 200, 221, 476
SfaNI GCATC 3 cut(s) 258, 498, 504
SinI GGWCC 1 cut(s) 519
Sse9I AATT 2 cut(s) 267, 541
SsiI CCGC 2 cut(s) 314, 487
SspMI CTAG 1 cut(s) 569
StyD4I CCNGG 2 cut(s) 54, 330
StyI CCWWGG 2 cut(s) 28, 372
TaiI ACGT 2 cut(s) 200, 476
TaqI TCGA 3 cut(s) 261, 425, 455
TasI AATT 2 cut(s) 267, 541
TatI WGTACW 1 cut(s) 435
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 1 cut(s) 23
Tru9I TTAA 1 cut(s) 23
TspDTI ATGAA 2 cut(s) 9, 525
TspGWI ACGGA 1 cut(s) 111
VpaK11BI GGWCC 1 cut(s) 519
XbaI TCTAGA 1 cut(s) 568
XspI CTAG 1 cut(s) 569
ZraI GACGTC 2 cut(s) 198, 474
ZrmI AGTACT 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.