pycom17g06190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002872
Physical Location & Seq
Reverse (-)
4353702 .. 4355711
2010 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g06190.4

Sequence Viewer

Length: 627 bp
ATGGGTATTTTTCCCAAGGGGCCCACCCCTGTTTTGTTCCCCCTGCCGTGTCCCTCCTTTCTTCTTTCTTCTTTTTCTTTTTTCTTCTTTTCCTTTCTTTTGTTTTGTTTTGTCTGCACCTTCTCCTTTCCTTCGAAACTCCCCCATTTCTCTCTTCTTCTTCCCCGTGAAAGACACACACACACCCACAACTACACCTACTCGAGGAAATCATCACCATCATCGCCATTCAACTGCATCTCTCTCTCTCTCTCTCTTTCCCCTGCTCGCGGCGCCGGGAGGACACGAACACCTCCGGCGAGTTTTGTGATTTTTCCAGTTGGGTTTTGGAGAGTTTGTGGCACGGGTGAAGGCTCACCCAAATTTTCCGGCGAGTGTGTGTTTTCCGAGGGAATTTCCGGCCACCACAGGCCGTTTCACGGCGAACCCAAGGGGCGATTATCTGTAGCATTTCCTTCTTCGCTAGATGGCATGGCTTTTCGACGGCGAAGTATTGACTTAGAATCGAGGCATACAATCCCGACGTCCAGGCACTTCCGCATCGGCATCTTCGAGTCCTCTTGGTGTAGGACCCACTCCTTTATTTCATTCAATTTTATATTATTTTCTTTTAGTGTTCTAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

23.41

Weight (kDa)

10.0

Isoelectric Point (pI)

55.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 527
AccB1I GGYRCC 1 cut(s) 272
AccII CGCG 1 cut(s) 270
AciI CCGC 2 cut(s) 270, 538
AcoI YGGCCR 1 cut(s) 400
AcsI RAATTY 2 cut(s) 362, 393
AcyI GRCGYC 2 cut(s) 273, 524
AfiI CCNNNNNNNGG 3 cut(s) 204, 269, 419
AgsI TTSAA 2 cut(s) 232, 592
AjnI CCWGG 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 202
AoxI GGCC 3 cut(s) 20, 400, 410
ApaI GGGCCC 1 cut(s) 24
ApoI RAATTY 2 cut(s) 362, 393
AspLEI GCGC 1 cut(s) 275
AspS9I GGNCC 3 cut(s) 20, 21, 570
AsuC2I CCSGG 1 cut(s) 277
AsuHPI GGTGA 3 cut(s) 207, 348, 359
AsuII TTCGAA 1 cut(s) 134
AvaI CYCGRG 1 cut(s) 202
AvaII GGWCC 1 cut(s) 570
BaeGI GKGCMC 1 cut(s) 24
BanI GGYRCC 1 cut(s) 272
BanII GRGCYC 1 cut(s) 24
BccI CCATC 2 cut(s) 226, 461
BceAI ACGGC 4 cut(s) 31, 397, 436, 500
BciT130I CCWGG 1 cut(s) 529
BcnI CCSGG 1 cut(s) 277
BfaI CTAG 2 cut(s) 464, 620
BfmI CTRYAG 1 cut(s) 444
BfoI RGCGCY 1 cut(s) 276
BisI GCNGC 1 cut(s) 271
BlsI GCNGC 1 cut(s) 272
Bme1390I CCNGG 2 cut(s) 277, 529
Bme18I GGWCC 1 cut(s) 570
BmeT110I CYCGRG 1 cut(s) 202
BmgT120I GGNCC 3 cut(s) 20, 21, 570
BmiI GGNNCC 4 cut(s) 21, 22, 274, 572
BmrFI CCNGG 2 cut(s) 277, 529
BmsI GCATC 3 cut(s) 246, 549, 555
Bpu14I TTCGAA 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 277
BsaHI GRCGYC 2 cut(s) 273, 524
BsaJI CCNNGG 3 cut(s) 15, 387, 429
BsaXI ACNNNNNCTCC 2 cut(s) 322, 352
Bsc4I CCNNNNNNNGG 3 cut(s) 204, 269, 419
Bse1I ACTGG 1 cut(s) 317
BseBI CCWGG 1 cut(s) 529
BseDI CCNNGG 3 cut(s) 15, 387, 429
BseLI CCNNNNNNNGG 3 cut(s) 204, 269, 419
BseNI ACTGG 1 cut(s) 317
BseSI GKGCMC 1 cut(s) 24
BsgI GTGCAG 1 cut(s) 100
Bsh1236I CGCG 1 cut(s) 270
BshFI GGCC 3 cut(s) 22, 402, 412
BshNI GGYRCC 1 cut(s) 272
BsiHKCI CYCGRG 1 cut(s) 202
BsiSI CCGG 4 cut(s) 276, 296, 369, 399
BslFI GGGAC 1 cut(s) 36
BslI CCNNNNNNNGG 3 cut(s) 204, 269, 419
BsmFI GGGAC 1 cut(s) 36
BsnI GGCC 3 cut(s) 22, 402, 412
BsoBI CYCGRG 1 cut(s) 202
Bsp119I TTCGAA 1 cut(s) 134
Bsp120I GGGCCC 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 24
BspACI CCGC 2 cut(s) 270, 538
BspANI GGCC 3 cut(s) 22, 402, 412
BspFNI CGCG 1 cut(s) 270
BspLI GGNNCC 4 cut(s) 21, 22, 274, 572
BspT104I TTCGAA 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 272
BsrI ACTGG 1 cut(s) 317
BssECI CCNNGG 3 cut(s) 15, 387, 429
BssNI GRCGYC 2 cut(s) 273, 524
BssT1I CCWWGG 2 cut(s) 15, 429
Bst2UI CCWGG 1 cut(s) 529
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 2 cut(s) 273, 524
BstBI TTCGAA 1 cut(s) 134
BstC8I GCNNGC 1 cut(s) 268
BstDEI CTNAG 1 cut(s) 499
BstENI CCTNNNNNAGG 1 cut(s) 202
BstFNI CGCG 1 cut(s) 270
BstH2I RGCGCY 1 cut(s) 276
BstHHI GCGC 1 cut(s) 275
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNI CCWGG 1 cut(s) 529
BstSCI CCNGG 2 cut(s) 275, 527
BstSFI CTRYAG 1 cut(s) 444
BstSLI GKGCMC 1 cut(s) 24
BstUI CGCG 1 cut(s) 270
BsuRI GGCC 3 cut(s) 22, 402, 412
BtgZI GCGATG 1 cut(s) 207
Cac8I GCNNGC 1 cut(s) 268
CfoI GCGC 1 cut(s) 275
Cfr13I GGNCC 3 cut(s) 20, 21, 570
CviAII CATG 1 cut(s) 472
CviJI RGCY 5 cut(s) 22, 354, 402, 412, 476
CviKI_1 RGCY 5 cut(s) 22, 354, 402, 412, 476
DdeI CTNAG 1 cut(s) 499
DinI GGCGCC 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 400
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco130I CCWWGG 2 cut(s) 15, 429
Eco24I GRGCYC 1 cut(s) 24
Eco47I GGWCC 1 cut(s) 570
Eco88I CYCGRG 1 cut(s) 202
EcoNI CCTNNNNNAGG 1 cut(s) 202
EcoO109I RGGNCCY 2 cut(s) 20, 570
EcoRII CCWGG 1 cut(s) 527
EcoT14I CCWWGG 2 cut(s) 15, 429
EcoT38I GRGCYC 1 cut(s) 24
EgeI GGCGCC 1 cut(s) 274
EheI GGCGCC 1 cut(s) 274
ErhI CCWWGG 2 cut(s) 15, 429
FaeI CATG 1 cut(s) 475
FaiI YATR 3 cut(s) 473, 513, 599
FaqI GGGAC 1 cut(s) 36
FatI CATG 1 cut(s) 471
Fnu4HI GCNGC 1 cut(s) 271
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 1 cut(s) 271
FspBI CTAG 2 cut(s) 464, 620
GlaI GCGC 1 cut(s) 274
GluI GCNGC 1 cut(s) 271
HaeII RGCGCY 1 cut(s) 276
HaeIII GGCC 3 cut(s) 22, 402, 412
HapII CCGG 4 cut(s) 276, 296, 369, 399
HhaI GCGC 1 cut(s) 275
Hin1I GRCGYC 2 cut(s) 273, 524
Hin1II CATG 1 cut(s) 475
Hin6I GCGC 1 cut(s) 273
HinP1I GCGC 1 cut(s) 273
HinfI GANTC 2 cut(s) 503, 554
HpaII CCGG 4 cut(s) 276, 296, 369, 399
HphI GGTGA 3 cut(s) 207, 348, 359
Hpy188I TCNGA 1 cut(s) 388
Hpy188III TCNNGA 2 cut(s) 520, 620
Hpy99I CGWCG 2 cut(s) 486, 526
HpyAV CCTTC 4 cut(s) 130, 141, 344, 465
HpyCH4IV ACGT 1 cut(s) 524
HpyCH4V TGCA 2 cut(s) 117, 237
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 1 cut(s) 499
HpySE526I ACGT 1 cut(s) 524
Hsp92I GRCGYC 2 cut(s) 273, 524
Hsp92II CATG 1 cut(s) 475
HspAI GCGC 1 cut(s) 273
KasI GGCGCC 1 cut(s) 272
LweI GCATC 3 cut(s) 246, 549, 555
MaeI CTAG 2 cut(s) 464, 620
MaeII ACGT 1 cut(s) 524
MboII GAAGA 8 cut(s) 53, 60, 76, 146, 149, 152, 450, 541
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 3 cut(s) 362, 393, 592
Mly113I GGCGCC 1 cut(s) 273
MlyI GAGTC 1 cut(s) 563
MnlI CCTC 7 cut(s) 64, 198, 273, 303, 382, 501, 568
MspI CCGG 4 cut(s) 276, 296, 369, 399
MspR9I CCNGG 2 cut(s) 277, 529
MvaI CCWGG 1 cut(s) 529
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 1 cut(s) 272
NarI GGCGCC 1 cut(s) 273
NciI CCSGG 1 cut(s) 277
NlaIII CATG 1 cut(s) 475
NlaIV GGNNCC 4 cut(s) 21, 22, 274, 572
NspV TTCGAA 1 cut(s) 134
PaeR7I CTCGAG 1 cut(s) 202
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PfeI GAWTC 1 cut(s) 503
PkrI GCNGC 1 cut(s) 272
PleI GAGTC 1 cut(s) 562
PluTI GGCGCC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 562
PpuMI RGGWCCY 1 cut(s) 570
Psp5II RGGWCCY 1 cut(s) 570
Psp6I CCWGG 1 cut(s) 527
PspGI CCWGG 1 cut(s) 527
PspN4I GGNNCC 4 cut(s) 21, 22, 274, 572
PspOMI GGGCCC 1 cut(s) 20
PspPI GGNCC 3 cut(s) 20, 21, 570
PspPPI RGGWCCY 1 cut(s) 570
PspXI VCTCGAGB 1 cut(s) 202
SatI GCNGC 1 cut(s) 271
Sau96I GGNCC 3 cut(s) 20, 21, 570
SchI GAGTC 1 cut(s) 563
ScrFI CCNGG 2 cut(s) 277, 529
SduI GDGCHC 1 cut(s) 24
SetI ASST 4 cut(s) 122, 200, 295, 527
SfaNI GCATC 3 cut(s) 246, 549, 555
SfcI CTRYAG 1 cut(s) 444
SfoI GGCGCC 1 cut(s) 274
Sfr274I CTCGAG 1 cut(s) 202
SfuI TTCGAA 1 cut(s) 134
SinI GGWCC 1 cut(s) 570
SlaI CTCGAG 1 cut(s) 202
SmlI CTYRAG 1 cut(s) 202
SmoI CTYRAG 1 cut(s) 202
Sse9I AATT 3 cut(s) 362, 393, 592
SsiI CCGC 2 cut(s) 270, 538
SspDI GGCGCC 1 cut(s) 272
SspMI CTAG 2 cut(s) 464, 620
StyD4I CCNGG 2 cut(s) 275, 527
StyI CCWWGG 2 cut(s) 15, 429
TaiI ACGT 1 cut(s) 527
TaqI TCGA 5 cut(s) 134, 203, 481, 506, 552
TasI AATT 3 cut(s) 362, 393, 592
TauI GCSGC 1 cut(s) 273
TfiI GAWTC 1 cut(s) 503
TspDTI ATGAA 1 cut(s) 576
VpaK11BI GGWCC 1 cut(s) 570
XagI CCTNNNNNAGG 1 cut(s) 202
XapI RAATTY 2 cut(s) 362, 393
XbaI TCTAGA 1 cut(s) 619
XcmI CCANNNNNNNNNTGG 1 cut(s) 324
XhoI CTCGAG 1 cut(s) 202
XspI CTAG 2 cut(s) 464, 620
ZraI GACGTC 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.