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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 256 |
| AciI | CCGC | 2 cut(s) | 308, 370 |
| AcoI | YGGCCR | 3 cut(s) | 330, 349, 400 |
| AcyI | GRCGYC | 1 cut(s) | 253 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 318, 339 |
| AjnI | CCWGG | 1 cut(s) | 386 |
| Alw21I | GWGCWC | 1 cut(s) | 170 |
| Alw26I | GTCTC | 2 cut(s) | 107, 274 |
| AoxI | GGCC | 4 cut(s) | 34, 330, 349, 400 |
| ApaI | GGGCCC | 1 cut(s) | 38 |
| AspS9I | GGNCC | 2 cut(s) | 34, 35 |
| AsuC2I | CCSGG | 1 cut(s) | 58 |
| AsuHPI | GGTGA | 1 cut(s) | 264 |
| BaeGI | GKGCMC | 1 cut(s) | 38 |
| BanII | GRGCYC | 1 cut(s) | 38 |
| Bbv12I | GWGCWC | 1 cut(s) | 170 |
| BceAI | ACGGC | 1 cut(s) | 356 |
| BciT130I | CCWGG | 1 cut(s) | 388 |
| BcnI | CCSGG | 1 cut(s) | 58 |
| BcoDI | GTCTC | 2 cut(s) | 107, 274 |
| Bme1390I | CCNGG | 2 cut(s) | 58, 388 |
| BmgT120I | GGNCC | 2 cut(s) | 34, 35 |
| BmiI | GGNNCC | 3 cut(s) | 35, 36, 62 |
| BmrFI | CCNGG | 2 cut(s) | 58, 388 |
| BmrI | ACTGGG | 1 cut(s) | 284 |
| BmuI | ACTGGG | 1 cut(s) | 284 |
| BpuMI | CCSGG | 1 cut(s) | 58 |
| BsaHI | GRCGYC | 1 cut(s) | 253 |
| BsaJI | CCNNGG | 5 cut(s) | 28, 56, 236, 338, 378 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 318, 339 |
| Bse118I | RCCGGY | 1 cut(s) | 351 |
| Bse1I | ACTGG | 1 cut(s) | 290 |
| BseBI | CCWGG | 1 cut(s) | 388 |
| BseDI | CCNNGG | 5 cut(s) | 28, 56, 236, 338, 378 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 318, 339 |
| BseMII | CTCAG | 1 cut(s) | 290 |
| BseNI | ACTGG | 1 cut(s) | 290 |
| BseSI | GKGCMC | 1 cut(s) | 38 |
| BshFI | GGCC | 4 cut(s) | 36, 332, 351, 402 |
| BsiHKAI | GWGCWC | 1 cut(s) | 170 |
| BsiSI | CCGG | 6 cut(s) | 58, 298, 329, 352, 367, 399 |
| BslFI | GGGAC | 1 cut(s) | 38 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 318, 339 |
| BsmAI | GTCTC | 2 cut(s) | 107, 274 |
| BsmFI | GGGAC | 1 cut(s) | 38 |
| BsnI | GGCC | 4 cut(s) | 36, 332, 351, 402 |
| Bsp120I | GGGCCC | 1 cut(s) | 34 |
| Bsp1286I | GDGCHC | 2 cut(s) | 38, 170 |
| BspACI | CCGC | 2 cut(s) | 308, 370 |
| BspANI | GGCC | 4 cut(s) | 36, 332, 351, 402 |
| BspCNI | CTCAG | 1 cut(s) | 289 |
| BspLI | GGNNCC | 3 cut(s) | 35, 36, 62 |
| BsrFI | RCCGGY | 1 cut(s) | 351 |
| BsrI | ACTGG | 1 cut(s) | 290 |
| BssAI | RCCGGY | 1 cut(s) | 351 |
| BssECI | CCNNGG | 5 cut(s) | 28, 56, 236, 338, 378 |
| BssNI | GRCGYC | 1 cut(s) | 253 |
| BssT1I | CCWWGG | 1 cut(s) | 28 |
| Bst2UI | CCWGG | 1 cut(s) | 388 |
| Bst6I | CTCTTC | 2 cut(s) | 112, 119 |
| BstACI | GRCGYC | 1 cut(s) | 253 |
| BstC8I | GCNNGC | 3 cut(s) | 303, 353, 357 |
| BstDEI | CTNAG | 1 cut(s) | 276 |
| BstDSI | CCRYGG | 3 cut(s) | 236, 338, 378 |
| BstMAI | GTCTC | 2 cut(s) | 107, 274 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 307 |
| BstNI | CCWGG | 1 cut(s) | 388 |
| BstSCI | CCNGG | 2 cut(s) | 56, 386 |
| BstSLI | GKGCMC | 1 cut(s) | 38 |
| BsuRI | GGCC | 4 cut(s) | 36, 332, 351, 402 |
| BtgI | CCRYGG | 3 cut(s) | 236, 338, 378 |
| Cac8I | GCNNGC | 3 cut(s) | 303, 353, 357 |
| Cfr10I | RCCGGY | 1 cut(s) | 351 |
| Cfr13I | GGNCC | 2 cut(s) | 34, 35 |
| CviAII | CATG | 1 cut(s) | 206 |
| CviJI | RGCY | 6 cut(s) | 36, 332, 351, 383, 391, 402 |
| CviKI_1 | RGCY | 6 cut(s) | 36, 332, 351, 383, 391, 402 |
| DdeI | CTNAG | 1 cut(s) | 276 |
| EaeI | YGGCCR | 3 cut(s) | 330, 349, 400 |
| Eam1104I | CTCTTC | 2 cut(s) | 112, 119 |
| EarI | CTCTTC | 2 cut(s) | 112, 119 |
| EciI | GGCGGA | 1 cut(s) | 385 |
| Eco130I | CCWWGG | 1 cut(s) | 28 |
| Eco24I | GRGCYC | 1 cut(s) | 38 |
| EcoO109I | RGGNCCY | 1 cut(s) | 34 |
| EcoRII | CCWGG | 1 cut(s) | 386 |
| EcoT14I | CCWWGG | 1 cut(s) | 28 |
| EcoT38I | GRGCYC | 1 cut(s) | 38 |
| ErhI | CCWWGG | 1 cut(s) | 28 |
| FaeI | CATG | 1 cut(s) | 209 |
| FaiI | YATR | 2 cut(s) | 207, 219 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 91, 123 |
| FaqI | GGGAC | 1 cut(s) | 38 |
| FatI | CATG | 1 cut(s) | 205 |
| FriOI | GRGCYC | 1 cut(s) | 38 |
| HaeIII | GGCC | 4 cut(s) | 36, 332, 351, 402 |
| HapII | CCGG | 6 cut(s) | 58, 298, 329, 352, 367, 399 |
| Hin1I | GRCGYC | 1 cut(s) | 253 |
| Hin1II | CATG | 1 cut(s) | 209 |
| HpaII | CCGG | 6 cut(s) | 58, 298, 329, 352, 367, 399 |
| HphI | GGTGA | 1 cut(s) | 264 |
| Hpy188I | TCNGA | 4 cut(s) | 96, 179, 279, 410 |
| HpyAV | CCTTC | 2 cut(s) | 74, 181 |
| HpyCH4IV | ACGT | 1 cut(s) | 253 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 307 |
| HpyF3I | CTNAG | 1 cut(s) | 276 |
| HpySE526I | ACGT | 1 cut(s) | 253 |
| Hsp92I | GRCGYC | 1 cut(s) | 253 |
| Hsp92II | CATG | 1 cut(s) | 209 |
| KroI | GCCGGC | 1 cut(s) | 351 |
| KroNI | GCCGGC | 1 cut(s) | 353 |
| LmnI | GCTCC | 1 cut(s) | 173 |
| MaeII | ACGT | 1 cut(s) | 253 |
| MboII | GAAGA | 3 cut(s) | 99, 106, 277 |
| MhlI | GDGCHC | 2 cut(s) | 38, 170 |
| MmeI | TCCRAC | 1 cut(s) | 202 |
| MnlI | CCTC | 4 cut(s) | 169, 185, 285, 312 |
| MroNI | GCCGGC | 1 cut(s) | 351 |
| MslI | CAYNNNNRTG | 1 cut(s) | 204 |
| MspI | CCGG | 6 cut(s) | 58, 298, 329, 352, 367, 399 |
| MspR9I | CCNGG | 2 cut(s) | 58, 388 |
| MvaI | CCWGG | 1 cut(s) | 388 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 307 |
| NaeI | GCCGGC | 1 cut(s) | 353 |
| NciI | CCSGG | 1 cut(s) | 58 |
| NgoMIV | GCCGGC | 1 cut(s) | 351 |
| NlaIII | CATG | 1 cut(s) | 209 |
| NlaIV | GGNNCC | 3 cut(s) | 35, 36, 62 |
| PdiI | GCCGGC | 1 cut(s) | 353 |
| Psp6I | CCWGG | 1 cut(s) | 386 |
| PspGI | CCWGG | 1 cut(s) | 386 |
| PspN4I | GGNNCC | 3 cut(s) | 35, 36, 62 |
| PspOMI | GGGCCC | 1 cut(s) | 34 |
| PspPI | GGNCC | 2 cut(s) | 34, 35 |
| RseI | CAYNNNNRTG | 1 cut(s) | 204 |
| Sau96I | GGNCC | 2 cut(s) | 34, 35 |
| ScrFI | CCNGG | 2 cut(s) | 58, 388 |
| SduI | GDGCHC | 2 cut(s) | 38, 170 |
| SetI | ASST | 3 cut(s) | 218, 256, 277 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 204 |
| SsiI | CCGC | 2 cut(s) | 308, 370 |
| StyD4I | CCNGG | 2 cut(s) | 56, 386 |
| StyI | CCWWGG | 1 cut(s) | 28 |
| TaiI | ACGT | 1 cut(s) | 256 |
| TaqI | TCGA | 1 cut(s) | 317 |
| ZraI | GACGTC | 1 cut(s) | 254 |