pycom14g14120

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002872
Physical Location & Seq
Reverse (-)
16900975 .. 16902046
1072 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g14120.2

Sequence Viewer

Length: 327 bp
ATGAAGAAACCACCTCGGAGACATTTCCCAGTTTTCCGGCGAGCACCGCCCCTTTCGAGGAAATTTCCGGCCAAACCACGGCGAGTTGGCCGGCGTGCAAGGTATCAATCTTTTCGTCTCGCTGATTTTTCGGCGGACACCCGTGGCTTCCAGGCTGTTTTTTGGCCAACTCCGACTACGATGACGGGAACCAAGGGGGCGTTTATCTGCGGCGTTTCCTTCTTCGCTAGATGGCGTGGCTTTTCGACGGCGAAGTACTGTGAGTGGACCCCTTCTAAAAATGCATGTTTTGATAGTAGAAATGCATACATGAAAAGCATGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

109

Amino Acids

12.58

Weight (kDa)

11.6

Isoelectric Point (pI)

55.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 47, 134, 210
AcoI YGGCCR 3 cut(s) 69, 88, 164
AcsI RAATTY 1 cut(s) 62
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 2 cut(s) 57, 78
AjnI CCWGG 1 cut(s) 150
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 2 cut(s) 13, 122
AoxI GGCC 3 cut(s) 69, 88, 164
ApoI RAATTY 1 cut(s) 62
AspS9I GGNCC 1 cut(s) 267
AvaII GGWCC 1 cut(s) 267
BalI TGGCCA 1 cut(s) 166
Bbv12I GWGCWC 1 cut(s) 46
BccI CCATC 1 cut(s) 225
BceAI ACGGC 2 cut(s) 95, 264
BciT130I CCWGG 1 cut(s) 152
BcoDI GTCTC 2 cut(s) 13, 122
BfaI CTAG 1 cut(s) 228
BisI GCNGC 1 cut(s) 211
BlsI GCNGC 1 cut(s) 212
BmcAI AGTACT 1 cut(s) 257
Bme1390I CCNGG 1 cut(s) 152
Bme18I GGWCC 1 cut(s) 267
BmgT120I GGNCC 1 cut(s) 267
BmiI GGNNCC 2 cut(s) 190, 269
BmrFI CCNGG 1 cut(s) 152
BmrI ACTGGG 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 23
BsaJI CCNNGG 4 cut(s) 14, 77, 142, 192
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 78
Bse118I RCCGGY 1 cut(s) 90
Bse1I ACTGG 1 cut(s) 29
BseBI CCWGG 1 cut(s) 152
BseDI CCNNGG 4 cut(s) 14, 77, 142, 192
BseLI CCNNNNNNNGG 2 cut(s) 57, 78
BseNI ACTGG 1 cut(s) 29
BshFI GGCC 3 cut(s) 71, 90, 166
BsiHKAI GWGCWC 1 cut(s) 46
BsiSI CCGG 3 cut(s) 37, 68, 91
BslI CCNNNNNNNGG 2 cut(s) 57, 78
BsmAI GTCTC 2 cut(s) 13, 122
BsmBI CGTCTC 1 cut(s) 122
BsnI GGCC 3 cut(s) 71, 90, 166
Bsp1286I GDGCHC 1 cut(s) 46
BspACI CCGC 3 cut(s) 47, 134, 210
BspANI GGCC 3 cut(s) 71, 90, 166
BspLI GGNNCC 2 cut(s) 190, 269
BsrFI RCCGGY 1 cut(s) 90
BsrI ACTGG 1 cut(s) 29
BssAI RCCGGY 1 cut(s) 90
BssECI CCNNGG 4 cut(s) 14, 77, 142, 192
BssT1I CCWWGG 1 cut(s) 192
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 1 cut(s) 260
BstC8I GCNNGC 3 cut(s) 42, 92, 96
BstDSI CCRYGG 2 cut(s) 77, 142
BstMAI GTCTC 2 cut(s) 13, 122
BstMWI GCNNNNNNNGC 1 cut(s) 46
BstNI CCWGG 1 cut(s) 152
BstNSI RCATGY 1 cut(s) 288
BstSCI CCNGG 1 cut(s) 150
BsuRI GGCC 3 cut(s) 71, 90, 166
BtgI CCRYGG 2 cut(s) 77, 142
Cac8I GCNNGC 3 cut(s) 42, 92, 96
Cfr10I RCCGGY 1 cut(s) 90
Cfr13I GGNCC 1 cut(s) 267
Csp6I GTAC 1 cut(s) 256
CviAII CATG 3 cut(s) 285, 310, 319
CviJI RGCY 6 cut(s) 71, 90, 147, 155, 166, 240
CviKI_1 RGCY 6 cut(s) 71, 90, 147, 155, 166, 240
CviQI GTAC 1 cut(s) 256
EaeI YGGCCR 3 cut(s) 69, 88, 164
EciI GGCGGA 1 cut(s) 149
Eco130I CCWWGG 1 cut(s) 192
Eco47I GGWCC 1 cut(s) 267
EcoRII CCWGG 1 cut(s) 150
EcoT14I CCWWGG 1 cut(s) 192
EcoT22I ATGCAT 2 cut(s) 286, 307
ErhI CCWWGG 1 cut(s) 192
Esp3I CGTCTC 1 cut(s) 122
FaeI CATG 3 cut(s) 288, 313, 322
FaiI YATR 4 cut(s) 286, 307, 311, 320
FatI CATG 3 cut(s) 284, 309, 318
Fnu4HI GCNGC 1 cut(s) 211
Fsp4HI GCNGC 1 cut(s) 211
FspBI CTAG 1 cut(s) 228
GluI GCNGC 1 cut(s) 211
HaeIII GGCC 3 cut(s) 71, 90, 166
HapII CCGG 3 cut(s) 37, 68, 91
Hin1II CATG 3 cut(s) 288, 313, 322
HpaII CCGG 3 cut(s) 37, 68, 91
Hpy166II GTNNAC 1 cut(s) 267
Hpy188I TCNGA 2 cut(s) 18, 174
Hpy8I GTNNAC 1 cut(s) 267
Hpy99I CGWCG 1 cut(s) 250
HpyAV CCTTC 2 cut(s) 229, 282
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4V TGCA 3 cut(s) 98, 284, 305
HpyF10VI GCNNNNNNNGC 1 cut(s) 46
Hsp92II CATG 3 cut(s) 288, 313, 322
KroI GCCGGC 1 cut(s) 90
KroNI GCCGGC 1 cut(s) 92
LpnPI CCDG 6 cut(s) 42, 50, 81, 104, 137, 164
MaeI CTAG 1 cut(s) 228
MboII GAAGA 2 cut(s) 16, 214
MhlI GDGCHC 1 cut(s) 46
MlsI TGGCCA 1 cut(s) 166
MluCI AATT 1 cut(s) 62
MluNI TGGCCA 1 cut(s) 166
MmeI TCCRAC 1 cut(s) 197
MnlI CCTC 2 cut(s) 24, 51
Mox20I TGGCCA 1 cut(s) 166
Mph1103I ATGCAT 2 cut(s) 286, 307
MroNI GCCGGC 1 cut(s) 90
MscI TGGCCA 1 cut(s) 166
Msp20I TGGCCA 1 cut(s) 166
MspI CCGG 3 cut(s) 37, 68, 91
MspR9I CCNGG 1 cut(s) 152
MvaI CCWGG 1 cut(s) 152
MwoI GCNNNNNNNGC 1 cut(s) 46
NaeI GCCGGC 1 cut(s) 92
NgoMIV GCCGGC 1 cut(s) 90
NlaIII CATG 3 cut(s) 288, 313, 322
NlaIV GGNNCC 2 cut(s) 190, 269
NsiI ATGCAT 2 cut(s) 286, 307
NspI RCATGY 1 cut(s) 288
PdiI GCCGGC 1 cut(s) 92
PkrI GCNGC 1 cut(s) 212
Psp6I CCWGG 1 cut(s) 150
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 2 cut(s) 190, 269
PspPI GGNCC 1 cut(s) 267
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SatI GCNGC 1 cut(s) 211
Sau96I GGNCC 1 cut(s) 267
ScaI AGTACT 1 cut(s) 257
ScrFI CCNGG 1 cut(s) 152
SduI GDGCHC 1 cut(s) 46
SetI ASST 2 cut(s) 16, 104
SinI GGWCC 1 cut(s) 267
Sse9I AATT 1 cut(s) 62
SsiI CCGC 3 cut(s) 47, 134, 210
SspMI CTAG 1 cut(s) 228
StyD4I CCNGG 1 cut(s) 150
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 1 cut(s) 260
TaqI TCGA 2 cut(s) 56, 245
TasI AATT 1 cut(s) 62
TatI WGTACW 1 cut(s) 255
TauI GCSGC 1 cut(s) 213
TspDTI ATGAA 2 cut(s) 17, 326
VpaK11BI GGWCC 1 cut(s) 267
XapI RAATTY 1 cut(s) 62
XceI RCATGY 1 cut(s) 288
XspI CTAG 1 cut(s) 228
ZrmI AGTACT 1 cut(s) 257
Zsp2I ATGCAT 2 cut(s) 286, 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.