pycom05g13520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002872
Physical Location & Seq
Reverse (-)
17085401 .. 17086828
1428 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g13520.4

Sequence Viewer

Length: 372 bp
ATGCCCACACCTATATTTTCCGTCGAGCACCGCCCTTTCGAGGCAATTTTCGGCCAAACCACGGCAAGTTGGCCGGCTTGCAAGTTTCCAGCGGACACCCGTGGCTTCCAGGCTGTTTTCCGGCCAACTCCGGCCACAATGATGGGAACTAAGGGGCCGTACTATGAAGTTGATTTTCAATATATGTATTTTCACTGGTTTTGTACTTACCTAGGCCTGGATTATCGGACATTCACCAGTGACTTAGAATCGAGGCATACAATCCCGACGTCCAGGCACTTCCGCATCGGCATCTTCGAGTCCACTTGGTGTAGAACCCACTCCTTGGTTTCATTCAATTTTATATTATTTTCTTTTAGTGTTCTAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

124

Amino Acids

14.44

Weight (kDa)

6.95

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 272
AccB7I CCANNNNNTGG 1 cut(s) 325
AciI CCGC 3 cut(s) 31, 92, 283
AcoI YGGCCR 4 cut(s) 52, 71, 122, 132
AcyI GRCGYC 1 cut(s) 269
AdeI CACNNNGTG 1 cut(s) 309
AfaI GTAC 2 cut(s) 161, 205
AfiI CCNNNNNNNGG 4 cut(s) 40, 61, 217, 325
AgsI TTSAA 2 cut(s) 179, 337
AjnI CCWGG 3 cut(s) 108, 216, 272
Alw21I GWGCWC 1 cut(s) 30
AoxI GGCC 6 cut(s) 52, 71, 122, 132, 155, 214
AspA2I CCTAGG 1 cut(s) 211
AspS9I GGNCC 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 226
AvrII CCTAGG 1 cut(s) 211
Bbv12I GWGCWC 1 cut(s) 30
BccI CCATC 1 cut(s) 136
BceAI ACGGC 2 cut(s) 78, 142
BciT130I CCWGG 3 cut(s) 110, 218, 274
BfaI CTAG 2 cut(s) 212, 365
BlnI CCTAGG 1 cut(s) 211
Bme1390I CCNGG 3 cut(s) 110, 218, 274
BmgT120I GGNCC 1 cut(s) 155
BmiI GGNNCC 1 cut(s) 156
BmrFI CCNGG 3 cut(s) 110, 218, 274
BmsI GCATC 2 cut(s) 294, 300
BsaHI GRCGYC 1 cut(s) 269
BsaJI CCNNGG 4 cut(s) 60, 100, 211, 324
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 61, 217, 325
Bse118I RCCGGY 1 cut(s) 73
Bse1I ACTGG 2 cut(s) 200, 237
BseBI CCWGG 3 cut(s) 110, 218, 274
BseDI CCNNGG 4 cut(s) 60, 100, 211, 324
BseLI CCNNNNNNNGG 4 cut(s) 40, 61, 217, 325
BseNI ACTGG 2 cut(s) 200, 237
BshFI GGCC 6 cut(s) 54, 73, 124, 134, 157, 216
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 3 cut(s) 74, 121, 131
BslI CCNNNNNNNGG 4 cut(s) 40, 61, 217, 325
BsnI GGCC 6 cut(s) 54, 73, 124, 134, 157, 216
Bsp1286I GDGCHC 1 cut(s) 30
BspACI CCGC 3 cut(s) 31, 92, 283
BspANI GGCC 6 cut(s) 54, 73, 124, 134, 157, 216
BspLI GGNNCC 1 cut(s) 156
BsrFI RCCGGY 1 cut(s) 73
BsrI ACTGG 2 cut(s) 200, 237
BssAI RCCGGY 1 cut(s) 73
BssECI CCNNGG 4 cut(s) 60, 100, 211, 324
BssNI GRCGYC 1 cut(s) 269
BssT1I CCWWGG 2 cut(s) 211, 324
Bst2UI CCWGG 3 cut(s) 110, 218, 274
BstACI GRCGYC 1 cut(s) 269
BstC8I GCNNGC 2 cut(s) 75, 79
BstDEI CTNAG 2 cut(s) 150, 244
BstDSI CCRYGG 2 cut(s) 60, 100
BstNI CCWGG 3 cut(s) 110, 218, 274
BstSCI CCNGG 3 cut(s) 108, 216, 272
BstXI CCANNNNNNTGG 1 cut(s) 142
BsuRI GGCC 6 cut(s) 54, 73, 124, 134, 157, 216
BtgI CCRYGG 2 cut(s) 60, 100
BtsIMutI CAGTG 2 cut(s) 193, 244
Cac8I GCNNGC 2 cut(s) 75, 79
Cfr10I RCCGGY 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 155
Csp6I GTAC 2 cut(s) 160, 204
CviJI RGCY 9 cut(s) 54, 73, 77, 105, 113, 124, 134, 157, 216
CviKI_1 RGCY 9 cut(s) 54, 73, 77, 105, 113, 124, 134, 157, 216
CviQI GTAC 2 cut(s) 160, 204
DdeI CTNAG 2 cut(s) 150, 244
DraIII CACNNNGTG 1 cut(s) 309
EaeI YGGCCR 4 cut(s) 52, 71, 122, 132
Eco130I CCWWGG 2 cut(s) 211, 324
Eco147I AGGCCT 1 cut(s) 216
EcoRII CCWGG 3 cut(s) 108, 216, 272
EcoT14I CCWWGG 2 cut(s) 211, 324
ErhI CCWWGG 2 cut(s) 211, 324
FaiI YATR 6 cut(s) 14, 165, 183, 185, 258, 344
FspBI CTAG 2 cut(s) 212, 365
HaeIII GGCC 6 cut(s) 54, 73, 124, 134, 157, 216
HapII CCGG 3 cut(s) 74, 121, 131
Hin1I GRCGYC 1 cut(s) 269
HinfI GANTC 2 cut(s) 248, 299
HpaII CCGG 3 cut(s) 74, 121, 131
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 1 cut(s) 303
Hpy188I TCNGA 1 cut(s) 228
Hpy188III TCNNGA 2 cut(s) 265, 365
Hpy8I GTNNAC 1 cut(s) 303
Hpy99I CGWCG 2 cut(s) 26, 271
HpyCH4IV ACGT 1 cut(s) 269
HpyCH4V TGCA 1 cut(s) 81
HpyF3I CTNAG 2 cut(s) 150, 244
HpySE526I ACGT 1 cut(s) 269
Hsp92I GRCGYC 1 cut(s) 269
KroI GCCGGC 1 cut(s) 73
KroNI GCCGGC 1 cut(s) 75
LweI GCATC 2 cut(s) 294, 300
MaeI CTAG 2 cut(s) 212, 365
MaeII ACGT 1 cut(s) 269
MaeIII GTNAC 1 cut(s) 239
MboII GAAGA 1 cut(s) 286
MhlI GDGCHC 1 cut(s) 30
MluCI AATT 2 cut(s) 45, 337
MlyI GAGTC 1 cut(s) 308
MnlI CCTC 2 cut(s) 34, 246
MroNI GCCGGC 1 cut(s) 73
MslI CAYNNNNRTG 1 cut(s) 140
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 3 cut(s) 74, 121, 131
MspR9I CCNGG 3 cut(s) 110, 218, 274
MvaI CCWGG 3 cut(s) 110, 218, 274
NaeI GCCGGC 1 cut(s) 75
NgoMIV GCCGGC 1 cut(s) 73
NlaIV GGNNCC 1 cut(s) 156
NmuCI GTSAC 1 cut(s) 239
PceI AGGCCT 1 cut(s) 216
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PdiI GCCGGC 1 cut(s) 75
PfeI GAWTC 1 cut(s) 248
PflMI CCANNNNNTGG 1 cut(s) 325
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
Psp6I CCWGG 3 cut(s) 108, 216, 272
PspGI CCWGG 3 cut(s) 108, 216, 272
PspN4I GGNNCC 1 cut(s) 156
PspPI GGNCC 1 cut(s) 155
RsaI GTAC 2 cut(s) 161, 205
RsaNI GTAC 2 cut(s) 160, 204
RseI CAYNNNNRTG 1 cut(s) 140
Sau96I GGNCC 1 cut(s) 155
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 3 cut(s) 110, 218, 274
SduI GDGCHC 1 cut(s) 30
SetI ASST 3 cut(s) 13, 213, 272
SfaNI GCATC 2 cut(s) 294, 300
SmiMI CAYNNNNRTG 1 cut(s) 140
Sse9I AATT 2 cut(s) 45, 337
SseBI AGGCCT 1 cut(s) 216
SsiI CCGC 3 cut(s) 31, 92, 283
SspMI CTAG 2 cut(s) 212, 365
StuI AGGCCT 1 cut(s) 216
StyD4I CCNGG 3 cut(s) 108, 216, 272
StyI CCWWGG 2 cut(s) 211, 324
TaiI ACGT 1 cut(s) 272
TaqI TCGA 4 cut(s) 24, 39, 251, 297
TasI AATT 2 cut(s) 45, 337
TatI WGTACW 1 cut(s) 203
TfiI GAWTC 1 cut(s) 248
TscAI CASTG 2 cut(s) 200, 244
TseFI GTSAC 1 cut(s) 239
Tsp45I GTSAC 1 cut(s) 239
TspDTI ATGAA 2 cut(s) 180, 321
TspGWI ACGGA 1 cut(s) 10
TspRI CASTG 2 cut(s) 200, 244
Van91I CCANNNNNTGG 1 cut(s) 325
XbaI TCTAGA 1 cut(s) 364
XmaJI CCTAGG 1 cut(s) 211
XspI CTAG 2 cut(s) 212, 365
ZraI GACGTC 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.