Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002601

Physical Location & Seq

Genomic Coordinates Reverse (-)

28997416 .. 29009548

Gene Structure 12133 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1161100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3342 bp

ATGCCCCGAAAACAAAAAACTTCAAAAGCATCCCAAAATAATGCATCAAATAAGGATCATATTGTACCAGTTGTATCCTCGATTTTCTTACGCAGGCTGCTTCTTCCTGGGGTTCATCATAATATTGCTTTGCGCTCAACCTTGCTGGATTATAACAGGCACTGGAGTGATTCAGATTTCCATTCTCTAACCGCCGATATGTTGAAGAAGGAAATTCTTTCCCTGATTGAACATGAGGGTGTCAGTGATAATTCAACGTCAATATTTTGCTGTTGGAAAAAATTCTGTTCTCGCTACTTCCAAAATTGGTGCAAGAGCAATGCACTATGTGGCTTGCTTTTGGATTCCCACAGGGGCACTTTTGGATTGATCAGAAAGAACTCAGTATCTCTTTTTCGTTGTTCGGAGAATATTGAGCGGCTTATTAATGGTATCTCAGGTACTTCTGATGATCTCATCCATCTTGTTACCATGGAGGCGGGTTTAATTCTTCAATTAGAGGTGCTCGTTAAAATGCTTAGATGTGTTGTTAATGTCAGCCAACAATTGGGAAAAACAGCTTCTGCTATATTTTATGAATCATTAGTTAGTGCACCACTCGTTATCTCAGCTGAGGAAATTACTCATCGCCTTTTGAAGAATTTAGAATCTGGATATGGTTCAACGGTAGCAATGCTTCATGTATCAGAGCATGGACCTGATGTTGCTTTGGAGAGAAACCTGGCTGATAAAAAAAAATTGAGGAAATTTTCTATTGGCATGCTGCTATCTCTTCATGCCTTGCACAAAGAATCTGGCACATGGAGTCGAATTTTAAAAGTGATTGAGGTTTATCTAAAAATTCTTAAACCTCGGAAAATTATACAAAAATCTGATGCTGAAATATCGTTGGATATTAGTGCTTCCATTTTGGTCCAGGCTACTTCTCAAGTTGCGAAGGTTATGTATGAGTCTGCCTTTGACATTCTTCTGTTTCTAAGCTATGTTGTGAGCATCAGTGGGCAGATCCAGATGCTGCATGATGATGTATCCAAAATTCAACTTGACTTGATTCCAAGGATTGAAAAAATCATTTCTGAGTGGCTTCTTATACATTTCTGTGCCACCACGCCATCTGAATCAGCTGAAATTGAAGATTTTAGCTCTCAGCTTTCAGTGTTACATATTGATGAAAACATGGGAAGAAGATCAAGGACTGAGAAGCTTGGAAAGTGTGAGTTCACTCTGGCTTTTTTATTCTTACCAAGCATTCGAACTTCCTCCGGAGACCAGGTCTGTATTGCCTTGAGAAGCATACCAGGCGTGAAGGACATTAGTATTTCAATGCGGGACTTTGTTAGCTGGATCATCTGGGGCCACACTGCAGAATCCTTTACCTTCCTCAAACGTGCAACAGATCTTGCTTTGATCCTTCTAAGGCATGGGCAGTATGATGCGGTTGAGCACCTACTCACTATGGTGGAGGCACATTTACAAAAGGAGAAGACATCTTATAGTATCCAAGATACAGAGGGAGGATGGTGCATACTTCATCATCTTTTAGGGTGCTGCTTCCTTGCTCAGGTACGCCGTGGGTTGCATGGAGTGCTGAAAGAGAGGAAAGTCACTGAAGCTGTTCGTTGTTTCTTCAGAGCTTCGTCTGGAAAAGGAACATCCCAGGCTTTACGGAGTTTGCTTCAGGAACCGGGGTCTCCAAATCTTGGTTTCACTGGTGATATATCAGCTTCCTCATGGAGACTGCATTATTACCAATGGGCTATGCAGATCTTTGAGCAATATAATATTAGCGAAGGCGCTTGTCAGTTTGCTCTCGCTGCGCTTGAGGAGGTAGAGGAAGCTAGTGTTAAAGATGAGCTGAATGGACAAGACTCGGTCAATGAGTCAGTATCTACCATCAAAGGACGACTTTGGGCAAATGTCTTCAAGTTTACTTTAGATCATAATCTCTATTGTGATGCATATTCTGCAGTAATTTCAAACCCAGATGAGGAGAGCAAGTACATCTGCTTGAGGCGTCTGATAATTGTTCTTTACGAGCGTGGTGCCATAAAGATTCTTTGTGGTGGTAAGCTACCTTTTGTTGGTCTTACAAAGAAGGTAGAGTACGAGCTTGCCAGGAAGGCAGATTGTTCAGGTATATTAGCAAAGCCAGACTTATATAAGTTGCTTTATGCATTTGAAATGCATCAACATAACTGGAGAAGAGCAGCATGCTACATGTACCTATATTCTGCTCGTTTGAGAAAAGAAACAGCTCTGAGAGATTACCAGCACACGTCATTGGCACTAAAGGAGATTCTGAATTGGCTCTCTGCTGCTATCAATGCACTTCATCTTGTTCATCCGGCCAACGCTTGGATTGATCCTCTACTCTGGACTGCCAGCAACAATGCTTATCCTCAAACAGGGAAATCTTATATTGATATTGAGAAGATTGAGGACGAGTTTATGCTTACTTCAGCAGAATATTTGCTTTCGTTGGCACATATCAAACCTGCATCTAGTGGTAAAGTGCAGAAGATTTGGACTCGCTATATCCAGGCCGTCCCATGTTTGTCTCATGGTTGGATTGGAAGAATTGTTGGAAGAACCCAGAAAGCACCACTAGAGGTTGTTGATCTCCTTATGCAGACAAATTTATATGATATGGCATTTACAGTTCTTCTAAGATTCTTAAAAGGTTCAGAGTTGAAGAGGGGAATGGAACGAGTTTTCTCTGCCATGTCACTGAGATGCTGTTCACATAGAGTTGATTCTTCATGGGTTAGGAATGAACACAGATCACGTGGTCTTCTGTTGACTTCATCAATGGCTGATGTGGCTGTTCATGGTTCACCTGATGTGGGTTCTACAAATGAACAATTTAAGGGAATCAGTCACTGGGGAACTCTTGAGCTTTACCTTGACAAGTATAAAGTGTTCCATGCCAGTTTACCACTAATTGTTGCTGAAACTCTTCTTCGTACGGATCCTCAAATTGAATTGCCTCTTTGGCTGGTAAAGATGTTCAAGGATGGTAGAAAGGACAAGACTTGGGGCATGACCGGCCAAGGATCAAATCCAGCATTATTATTCCAACTATATGTTGATTACGGTCGATACAGAGAAGCTACAATTTTTTTGCTCGAGTACATAGGATTGTTTGCATCAATGAGGCCGGCTGACATAATCAAGCGTAAAAGACCACTTGGAGTCTGGTTTCCATACACAGGAATTCAGCGTCTGTGGTGCCAACTTGAGGAATTGATCAGTTCGGGCCACATGGTAGATGAATGTAACAAGCTCAAGAATTTATTATGTGGAGCTTTGCTGAGCCATCTCGAACTGGTGAAAGTAGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0008104 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016973 GO:0031090 GO:0031503 GO:0031965 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0034613 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

DUF5311 Nup160

Protein Analysis

1114

Amino Acids

126.01

Weight (kDa)

8.59

Isoelectric Point (pI)

38.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_Nup120_160	PF11715	18 - 139	6.5e-23	Nucleoporin Nup120/160, beta-propeller domain
NUP160_helical_2	PF17238	165 - 350	5.8e-79	NUP160, helical domain
TPR_NUP160_120_M	PF23354	529 - 779	3.5e-35	NUP160/120 middle TPR
HEAT_Nup120	PF23300	530 - 678	3.8e-14	Nucleoporin Nup120, HEAT repeats
TPR_Nup160_C	PF23347	870 - 1098	3.1e-07	Nup160 C-terminal TPR

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002601)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33410 AT1G33410
fragaria_vesca	FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400
malus_domestica	MD00G1034800.v1.1 MD05G1063300.v1.1 MD05G1161100.v1.1 MD05G1162300.v1.1 MD05G1162400.v1.1 MD07G1280900.v1.1 MD08G1158600.v1.1 MD10G1009800.v1.1 MD10G1160000.v1.1
prunus_persica	Prupe.5G022000_v2.0.a1
pyrus_communis	pycom10g00600
rosa_chinensis	RchiOBHm_Chr2g0126921 RchiOBHm_Chr4g0402211
rosa_laevigata	RLG00000009106 RLG00000018928
rosa_multiflora	Rmu_sc0000007.1_g000030 Rmu_sc0001405.1_g000051
rosa_roxburghii	Rroxscaffold_1G00047890 Rroxscaffold_2G00116920 Rroxscaffold_5G00346940
rosa_rugosa	Rorug02G0270100 Rorug02G0270200
rosa_samantha	Rh2AG322500 Rh2BG330700 Rh2CG310600 Rh2DG350000 Rh4BG104100 Rh4CG117900 Rh4DG101500
rosa_wichuraiana	Rw2G026160 Rw4G008910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	153
Acc36I	ACCTGC	1 cut(s)	2503
AccB1I	GGYRCC	2 cut(s)	2042, 3228
AccB7I	CCANNNNNTGG	3 cut(s)	547, 1700, 2355
AccBSI	CCGCTC	1 cut(s)	418
AccI	GTMKAC	1 cut(s)	3336
AccIII	TCCGGA	1 cut(s)	1262
AciI	CCGC	5 cut(s)	192, 418, 479, 1327, 1436
AclWI	GGATC	8 cut(s)	63, 1000, 1352, 1402, 2357, 2963, 2976, 3061
AcoI	YGGCCR	2 cut(s)	2346, 3046
AcuI	CTGAAG	4 cut(s)	1612, 1629, 1661, 2442
AcvI	CACGTG	1 cut(s)	2786
AcyI	GRCGYC	1 cut(s)	2014
AdeI	CACNNNGTG	1 cut(s)	329
AfaI	GTAC	8 cut(s)	66, 442, 1566, 2000, 2105, 2222, 2965, 3131
AflIII	ACRYGT	2 cut(s)	2217, 2274
AjiI	CACGTC	1 cut(s)	2277
AjnI	CCWGG	8 cut(s)	106, 720, 915, 1269, 1297, 1656, 2114, 2538
AleI	CACNNNNGTG	2 cut(s)	165, 1457
Alw21I	GWGCWC	3 cut(s)	507, 595, 1446
Alw26I	GTCTC	4 cut(s)	1260, 1695, 1729, 2562
Alw44I	GTGCAC	1 cut(s)	591
AlwI	GGATC	8 cut(s)	63, 1000, 1352, 1402, 2357, 2963, 2976, 3061
AlwNI	CAGNNNCTG	5 cut(s)	162, 563, 1015, 2880, 3223
Ama87I	CYCGRG	1 cut(s)	3125
Aor13HI	TCCGGA	1 cut(s)	1262
AoxI	GGCC	6 cut(s)	1354, 2346, 2541, 3046, 3155, 3256
ApaLI	GTGCAC	1 cut(s)	591
ApeKI	GCWGC	7 cut(s)	97, 763, 1015, 1548, 1814, 2207, 2315
AseI	ATTAAT	1 cut(s)	426
Asp700I	GAANNNNTTC	3 cut(s)	281, 1614, 2955
AspLEI	GCGC	3 cut(s)	135, 1796, 1819
AspS9I	GGNCC	4 cut(s)	695, 913, 1354, 3256
AsuC2I	CCSGG	1 cut(s)	1686
AsuHPI	GGTGA	3 cut(s)	1724, 2826, 3340
AsuII	TTCGAA	1 cut(s)	1252
AvaI	CYCGRG	1 cut(s)	3125
AvaII	GGWCC	2 cut(s)	695, 913
BaeGI	GKGCMC	2 cut(s)	359, 595
BamHI	GGATCC	1 cut(s)	2968
BanI	GGYRCC	2 cut(s)	2042, 3228
BarI	GAAGNNNNNNTAC	4 cut(s)	660, 692, 2087, 2119
BbrPI	CACGTG	1 cut(s)	2786
BbsI	GAAGAC	3 cut(s)	1490, 1912, 2783
Bbv12I	GWGCWC	3 cut(s)	507, 595, 1446
BbvCI	CCTCAGC	1 cut(s)	612
BbvI	GCAGC	7 cut(s)	84, 750, 1002, 1535, 1801, 2219, 2302
BccI	CCATC	6 cut(s)	468, 1120, 1512, 1901, 3008, 3324
BceAI	ACGGC	2 cut(s)	1554, 2528
BcgI	CGANNNNNNTGC	2 cut(s)	2024, 2058
BciT130I	CCWGG	8 cut(s)	108, 722, 917, 1271, 1299, 1658, 2116, 2540
BciVI	GTATCC	3 cut(s)	85, 1039, 1508
BclI	TGATCA	2 cut(s)	369, 3246
BcnI	CCSGG	1 cut(s)	1686
BcoDI	GTCTC	4 cut(s)	1260, 1695, 1729, 2562
BfaI	CTAG	4 cut(s)	1839, 2502, 2606, 3340
BfmI	CTRYAG	2 cut(s)	1362, 1965
BfoI	RGCGCY	1 cut(s)	1797
BfuAI	ACCTGC	1 cut(s)	2503
BfuI	GTATCC	3 cut(s)	85, 1039, 1508
BglI	GCCNNNNNGGC	2 cut(s)	2120, 2992
BglII	AGATCT	2 cut(s)	1396, 1764
BisI	GCNGC	8 cut(s)	98, 419, 764, 1016, 1549, 1815, 2208, 2316
BlpI	GCTNAGC	1 cut(s)	3311
BlsI	GCNGC	8 cut(s)	99, 420, 765, 1017, 1550, 1816, 2209, 2317
Bme1390I	CCNGG	9 cut(s)	108, 722, 917, 1271, 1299, 1658, 1686, 2116, 2540
Bme18I	GGWCC	2 cut(s)	695, 913
BmeT110I	CYCGRG	1 cut(s)	3125
BmgBI	CACGTC	1 cut(s)	2277
BmgT120I	GGNCC	4 cut(s)	695, 913, 1354, 3256
BmiI	GGNNCC	5 cut(s)	1355, 1683, 2044, 2970, 3230
BmrFI	CCNGG	9 cut(s)	108, 722, 917, 1271, 1299, 1658, 1686, 2116, 2540
BmrI	ACTGGG	1 cut(s)	2890
BmuI	ACTGGG	1 cut(s)	2890
BpiI	GAAGAC	3 cut(s)	1490, 1912, 2783
BpmI	CTGGAG	2 cut(s)	184, 2218
Bpu10I	CCTNAGC	2 cut(s)	612, 1560
Bpu1102I	GCTNAGC	1 cut(s)	3311
Bpu14I	TTCGAA	1 cut(s)	1252
BpuEI	CTTGAG	7 cut(s)	912, 1306, 1841, 2029, 2912, 3257, 3269
BpuMI	CCSGG	1 cut(s)	1686
BsaAI	YACGTR	1 cut(s)	2786
BsaBI	GATNNNNATC	1 cut(s)	1941
BsaHI	GRCGYC	1 cut(s)	2014
BsaI	GGTCTC	2 cut(s)	1260, 1695
BsaJI	CCNNGG	8 cut(s)	107, 471, 851, 1055, 1570, 1656, 1685, 3049
BsaWI	WCCGGW	1 cut(s)	1262
BsaXI	ACNNNNNCTCC	4 cut(s)	1257, 1287, 1982, 2012
Bse118I	RCCGGY	2 cut(s)	3044, 3157
Bse1I	ACTGG	7 cut(s)	68, 167, 1714, 2201, 2885, 2928, 3330
Bse3DI	GCAATG	2 cut(s)	325, 678
Bse8I	GATNNNNATC	1 cut(s)	1941
BseAI	TCCGGA	1 cut(s)	1262
BseBI	CCWGG	8 cut(s)	108, 722, 917, 1271, 1299, 1658, 2116, 2540
BseDI	CCNNGG	8 cut(s)	107, 471, 851, 1055, 1570, 1656, 1685, 3049
BseGI	GGATG	6 cut(s)	29, 456, 1523, 1652, 2341, 3019
BseJI	GATNNNNATC	1 cut(s)	1941
BseMI	GCAATG	2 cut(s)	325, 678
BseNI	ACTGG	7 cut(s)	68, 167, 1714, 2201, 2885, 2928, 3330
BseRI	GAGGAG	2 cut(s)	1838, 2003
BseSI	GKGCMC	2 cut(s)	359, 595
BseXI	GCAGC	7 cut(s)	84, 750, 1002, 1535, 1801, 2219, 2302
BsgI	GTGCAG	1 cut(s)	2534
Bsh1285I	CGRYCG	1 cut(s)	3097
BshFI	GGCC	6 cut(s)	1356, 2348, 2543, 3048, 3157, 3258
BshNI	GGYRCC	2 cut(s)	2042, 3228
BsiEI	CGRYCG	1 cut(s)	3097
BsiHKAI	GWGCWC	3 cut(s)	507, 595, 1446
BsiHKCI	CYCGRG	1 cut(s)	3125
BsiSI	CCGG	5 cut(s)	1263, 1685, 2345, 3045, 3158
BsiWI	CGTACG	1 cut(s)	2963
BslFI	GGGAC	2 cut(s)	1343, 2531
BsmAI	GTCTC	4 cut(s)	1260, 1695, 1729, 2562
BsmFI	GGGAC	2 cut(s)	1343, 2531
BsmI	GAATGC	1 cut(s)	1248
BsnI	GGCC	6 cut(s)	1356, 2348, 2543, 3048, 3157, 3258
Bso31I	GGTCTC	2 cut(s)	1260, 1695
BsoBI	CYCGRG	1 cut(s)	3125
Bsp119I	TTCGAA	1 cut(s)	1252
Bsp1286I	GDGCHC	4 cut(s)	359, 507, 595, 1446
Bsp13I	TCCGGA	1 cut(s)	1262
Bsp1720I	GCTNAGC	1 cut(s)	3311
Bsp19I	CCATGG	1 cut(s)	471
BspACI	CCGC	5 cut(s)	192, 418, 479, 1327, 1436
BspANI	GGCC	6 cut(s)	1356, 2348, 2543, 3048, 3157, 3258
BspEI	TCCGGA	1 cut(s)	1262
BspLI	GGNNCC	5 cut(s)	1355, 1683, 2044, 2970, 3230
BspMAI	CTGCAG	2 cut(s)	1366, 1969
BspMI	ACCTGC	1 cut(s)	2503
BspPI	GGATC	8 cut(s)	63, 1000, 1352, 1402, 2357, 2963, 2976, 3061
BspQI	GCTCTTC	1 cut(s)	2197
BspT104I	TTCGAA	1 cut(s)	1252
BspT107I	GGYRCC	2 cut(s)	2042, 3228
BspTNI	GGTCTC	2 cut(s)	1260, 1695
BsrBI	CCGCTC	1 cut(s)	418
BsrDI	GCAATG	2 cut(s)	325, 678
BsrFI	RCCGGY	2 cut(s)	3044, 3157
BsrI	ACTGG	7 cut(s)	68, 167, 1714, 2201, 2885, 2928, 3330
BssAI	RCCGGY	2 cut(s)	3044, 3157
BssECI	CCNNGG	8 cut(s)	107, 471, 851, 1055, 1570, 1656, 1685, 3049
BssNI	GRCGYC	1 cut(s)	2014
BssT1I	CCWWGG	3 cut(s)	471, 1055, 3049
Bst2UI	CCWGG	8 cut(s)	108, 722, 917, 1271, 1299, 1658, 2116, 2540
Bst4CI	ACNGT	3 cut(s)	667, 2659, 3095
Bst6I	CTCTTC	4 cut(s)	777, 2197, 2687, 2961
BstACI	GRCGYC	1 cut(s)	2014
BstAPI	GCANNNNNTGC	2 cut(s)	1585, 1964
BstBAI	YACGTR	1 cut(s)	2786
BstBI	TTCGAA	1 cut(s)	1252
BstC8I	GCNNGC	7 cut(s)	95, 335, 761, 2112, 2212, 2383, 3159
BstDSI	CCRYGG	2 cut(s)	471, 1570
BstENI	CCTNNNNNAGG	2 cut(s)	1559, 2403
BstF5I	GGATG	6 cut(s)	29, 456, 1523, 1652, 2341, 3019
BstH2I	RGCGCY	1 cut(s)	1797
BstHHI	GCGC	3 cut(s)	135, 1796, 1819
BstMAI	GTCTC	4 cut(s)	1260, 1695, 1729, 2562
BstMCI	CGRYCG	1 cut(s)	3097
BstMWI	GCNNNNNNNGC	9 cut(s)	1299, 1585, 1814, 1964, 2120, 2324, 2819, 2992, 3045
BstNI	CCWGG	8 cut(s)	108, 722, 917, 1271, 1299, 1658, 2116, 2540
BstNSI	RCATGY	3 cut(s)	763, 2214, 2221
BstSCI	CCNGG	9 cut(s)	106, 720, 915, 1269, 1297, 1656, 1684, 2114, 2538
BstSFI	CTRYAG	2 cut(s)	1362, 1965
BstSLI	GKGCMC	2 cut(s)	359, 595
BstV1I	GCAGC	7 cut(s)	84, 750, 1002, 1535, 1801, 2219, 2302
BstV2I	GAAGAC	3 cut(s)	1490, 1912, 2783
BstX2I	RGATCY	4 cut(s)	1005, 1396, 1764, 2968
BstYI	RGATCY	4 cut(s)	1005, 1396, 1764, 2968
BsuI	GTATCC	3 cut(s)	85, 1039, 1508
BsuRI	GGCC	6 cut(s)	1356, 2348, 2543, 3048, 3157, 3258
BtgI	CCRYGG	2 cut(s)	471, 1570
BtgZI	GCGATG	1 cut(s)	611
BtrI	CACGTC	1 cut(s)	2277
BtsCI	GGATG	6 cut(s)	29, 456, 1523, 1652, 2341, 3019
BtsI	GCAGTG	1 cut(s)	1359
BtsIMutI	CAGTG	9 cut(s)	160, 250, 1003, 1161, 1359, 1605, 1707, 2726, 2878
BveI	ACCTGC	1 cut(s)	2503
Cac8I	GCNNGC	7 cut(s)	95, 335, 761, 2112, 2212, 2383, 3159
CaiI	CAGNNNCTG	5 cut(s)	162, 563, 1015, 2880, 3223
CfoI	GCGC	3 cut(s)	135, 1796, 1819
Cfr10I	RCCGGY	2 cut(s)	3044, 3157
Cfr13I	GGNCC	4 cut(s)	695, 913, 1354, 3256
CseI	GACGC	2 cut(s)	2003, 3209
CsiI	ACCWGGT	1 cut(s)	1269
Csp6I	GTAC	8 cut(s)	65, 441, 1565, 1999, 2104, 2221, 2964, 3130
CviQI	GTAC	8 cut(s)	65, 441, 1565, 1999, 2104, 2221, 2964, 3130
DraI	TTTAAA	1 cut(s)	816
DraIII	CACNNNGTG	1 cut(s)	329
EaeI	YGGCCR	2 cut(s)	2346, 3046
Eam1104I	CTCTTC	4 cut(s)	777, 2197, 2687, 2961
EarI	CTCTTC	4 cut(s)	777, 2197, 2687, 2961
Eco130I	CCWWGG	3 cut(s)	471, 1055, 3049
Eco31I	GGTCTC	2 cut(s)	1260, 1695
Eco47I	GGWCC	2 cut(s)	695, 913
Eco57I	CTGAAG	4 cut(s)	1612, 1629, 1661, 2442
Eco72I	CACGTG	1 cut(s)	2786
Eco88I	CYCGRG	1 cut(s)	3125
EcoNI	CCTNNNNNAGG	2 cut(s)	1559, 2403
EcoRI	GAATTC	1 cut(s)	3213
EcoRII	CCWGG	8 cut(s)	106, 720, 915, 1269, 1297, 1656, 2114, 2538
EcoT14I	CCWWGG	3 cut(s)	471, 1055, 3049
EcoT22I	ATGCAT	4 cut(s)	46, 1960, 2176, 2187
ErhI	CCWWGG	3 cut(s)	471, 1055, 3049
FalI	AAGNNNNNCTT	4 cut(s)	1890, 1922, 2138, 2170
FaqI	GGGAC	2 cut(s)	1343, 2531
FauI	CCCGC	2 cut(s)	472, 1320
FbaI	TGATCA	2 cut(s)	369, 3246
FblI	GTMKAC	1 cut(s)	3336
Fnu4HI	GCNGC	8 cut(s)	98, 419, 764, 1016, 1549, 1815, 2208, 2316
FokI	GGATG	6 cut(s)	16, 443, 1530, 1639, 2328, 3026
Fsp4HI	GCNGC	8 cut(s)	98, 419, 764, 1016, 1549, 1815, 2208, 2316
FspBI	CTAG	4 cut(s)	1839, 2502, 2606, 3340
GlaI	GCGC	3 cut(s)	134, 1795, 1818
GluI	GCNGC	8 cut(s)	98, 419, 764, 1016, 1549, 1815, 2208, 2316
GsuI	CTGGAG	2 cut(s)	184, 2218
HaeII	RGCGCY	1 cut(s)	1797
HaeIII	GGCC	6 cut(s)	1356, 2348, 2543, 3048, 3157, 3258
HapII	CCGG	5 cut(s)	1263, 1685, 2345, 3045, 3158
HgaI	GACGC	2 cut(s)	2003, 3209
HhaI	GCGC	3 cut(s)	135, 1796, 1819
Hin1I	GRCGYC	1 cut(s)	2014
Hin6I	GCGC	3 cut(s)	133, 1794, 1817
HinP1I	GCGC	3 cut(s)	133, 1794, 1817
HincII	GTYRAC	1 cut(s)	2799
HindII	GTYRAC	1 cut(s)	2799
HindIII	AAGCTT	1 cut(s)	1202
HpaII	CCGG	5 cut(s)	1263, 1685, 2345, 3045, 3158
HphI	GGTGA	3 cut(s)	1724, 2826, 3340
Hpy166II	GTNNAC	8 cut(s)	593, 1221, 1929, 2741, 2799, 2834, 2933, 3337
Hpy188III	TCNNGA	9 cut(s)	651, 1009, 1263, 1641, 1679, 2374, 2891, 3286, 3320
Hpy8I	GTNNAC	8 cut(s)	593, 1221, 1929, 2741, 2799, 2834, 2933, 3337
HpyAV	CCTTC	8 cut(s)	202, 931, 1300, 1387, 1421, 1784, 2089, 2113
HpyCH4III	ACNGT	3 cut(s)	667, 2659, 3095
HpyCH4IV	ACGT	4 cut(s)	257, 1387, 2276, 2785
HpyF10VI	GCNNNNNNNGC	9 cut(s)	1299, 1585, 1814, 1964, 2120, 2324, 2819, 2992, 3045
HpySE526I	ACGT	4 cut(s)	257, 1387, 2276, 2785
Hsp92I	GRCGYC	1 cut(s)	2014
HspAI	GCGC	3 cut(s)	133, 1794, 1817
Kpn2I	TCCGGA	1 cut(s)	1262
KroI	GCCGGC	1 cut(s)	3157
KroNI	GCCGGC	1 cut(s)	3159
Ksp22I	TGATCA	2 cut(s)	369, 3246
LguI	GCTCTTC	1 cut(s)	2197
LmnI	GCTCC	1 cut(s)	3302
Lsp1109I	GCAGC	7 cut(s)	84, 750, 1002, 1535, 1801, 2219, 2302
MabI	ACCWGGT	1 cut(s)	1269
MaeI	CTAG	4 cut(s)	1839, 2502, 2606, 3340
MaeII	ACGT	4 cut(s)	257, 1387, 2276, 2785
MaeIII	GTNAC	6 cut(s)	466, 1158, 1603, 2724, 2876, 3275
MbiI	CCGCTC	1 cut(s)	418
MfeI	CAATTG	1 cut(s)	545
MflI	RGATCY	4 cut(s)	1005, 1396, 1764, 2968
MhlI	GDGCHC	4 cut(s)	359, 507, 595, 1446
MlyI	GAGTC	6 cut(s)	814, 959, 1862, 1889, 2521, 3201
MmeI	TCCRAC	5 cut(s)	254, 870, 2546, 2563, 3100
Mph1103I	ATGCAT	4 cut(s)	46, 1960, 2176, 2187
MroI	TCCGGA	1 cut(s)	1262
MroNI	GCCGGC	1 cut(s)	3157
MroXI	GAANNNNTTC	3 cut(s)	281, 1614, 2955
MseI	TTAA	9 cut(s)	426, 485, 510, 531, 815, 846, 1845, 2675, 2865
MslI	CAYNNNNRTG	7 cut(s)	165, 237, 1023, 1098, 1167, 1457, 2731
MspA1I	CMGCKG	2 cut(s)	611, 1124
MspI	CCGG	5 cut(s)	1263, 1685, 2345, 3045, 3158
MspR9I	CCNGG	9 cut(s)	108, 722, 917, 1271, 1299, 1658, 1686, 2116, 2540
MunI	CAATTG	1 cut(s)	545
Mva1269I	GAATGC	1 cut(s)	1248
MvaI	CCWGG	8 cut(s)	108, 722, 917, 1271, 1299, 1658, 2116, 2540
MwoI	GCNNNNNNNGC	9 cut(s)	1299, 1585, 1814, 1964, 2120, 2324, 2819, 2992, 3045
NaeI	GCCGGC	1 cut(s)	3159
NciI	CCSGG	1 cut(s)	1686
NcoI	CCATGG	1 cut(s)	471
NgoMIV	GCCGGC	1 cut(s)	3157
NlaIV	GGNNCC	5 cut(s)	1355, 1683, 2044, 2970, 3230
NmuCI	GTSAC	3 cut(s)	1603, 2724, 2876
NsiI	ATGCAT	4 cut(s)	46, 1960, 2176, 2187
NspI	RCATGY	3 cut(s)	763, 2214, 2221
NspV	TTCGAA	1 cut(s)	1252
OliI	CACNNNNGTG	2 cut(s)	165, 1457
PaeI	GCATGC	2 cut(s)	763, 2214
PaeR7I	CTCGAG	1 cut(s)	3125
PciI	ACATGT	1 cut(s)	2217
PciSI	GCTCTTC	1 cut(s)	2197
PctI	GAATGC	1 cut(s)	1248
PdiI	GCCGGC	1 cut(s)	3159
PdmI	GAANNNNTTC	3 cut(s)	281, 1614, 2955
Pfl23II	CGTACG	1 cut(s)	2963
PflFI	GACNNNGTC	2 cut(s)	1271, 1871
PflMI	CCANNNNNTGG	3 cut(s)	547, 1700, 2355
PkrI	GCNGC	8 cut(s)	99, 420, 765, 1017, 1550, 1816, 2209, 2317
PleI	GAGTC	6 cut(s)	813, 958, 1862, 1888, 2521, 3200
PmaCI	CACGTG	1 cut(s)	2786
PmlI	CACGTG	1 cut(s)	2786
PpsI	GAGTC	6 cut(s)	813, 958, 1862, 1888, 2521, 3200
Ppu21I	YACGTR	1 cut(s)	2786
PscI	ACATGT	1 cut(s)	2217
PshBI	ATTAAT	1 cut(s)	426
PsiI	TTATAA	1 cut(s)	153
Psp6I	CCWGG	8 cut(s)	106, 720, 915, 1269, 1297, 1656, 2114, 2538
PspCI	CACGTG	1 cut(s)	2786
PspGI	CCWGG	8 cut(s)	106, 720, 915, 1269, 1297, 1656, 2114, 2538
PspLI	CGTACG	1 cut(s)	2963
PspN4I	GGNNCC	5 cut(s)	1355, 1683, 2044, 2970, 3230
PspPI	GGNCC	4 cut(s)	695, 913, 1354, 3256
PspXI	VCTCGAGB	1 cut(s)	3125
PsrI	GAACNNNNNNTAC	2 cut(s)	2903, 2935
PstI	CTGCAG	2 cut(s)	1366, 1969
PstNI	CAGNNNCTG	5 cut(s)	162, 563, 1015, 2880, 3223
PsuI	RGATCY	4 cut(s)	1005, 1396, 1764, 2968
PsyI	GACNNNGTC	2 cut(s)	1271, 1871
PvuII	CAGCTG	2 cut(s)	611, 1124
RsaI	GTAC	8 cut(s)	66, 442, 1566, 2000, 2105, 2222, 2965, 3131
RsaNI	GTAC	8 cut(s)	65, 441, 1565, 1999, 2104, 2221, 2964, 3130
RseI	CAYNNNNRTG	7 cut(s)	165, 237, 1023, 1098, 1167, 1457, 2731
SapI	GCTCTTC	1 cut(s)	2197
SaqAI	TTAA	9 cut(s)	426, 485, 510, 531, 815, 846, 1845, 2675, 2865
SatI	GCNGC	8 cut(s)	98, 419, 764, 1016, 1549, 1815, 2208, 2316
Sau96I	GGNCC	4 cut(s)	695, 913, 1354, 3256
SchI	GAGTC	6 cut(s)	814, 959, 1862, 1889, 2521, 3201
ScrFI	CCNGG	9 cut(s)	108, 722, 917, 1271, 1299, 1658, 1686, 2116, 2540
SduI	GDGCHC	4 cut(s)	359, 507, 595, 1446
SexAI	ACCWGGT	1 cut(s)	1269
SfcI	CTRYAG	2 cut(s)	1362, 1965
Sfr274I	CTCGAG	1 cut(s)	3125
SfuI	TTCGAA	1 cut(s)	1252
SinI	GGWCC	2 cut(s)	695, 913
SlaI	CTCGAG	1 cut(s)	3125
SmiMI	CAYNNNNRTG	7 cut(s)	165, 237, 1023, 1098, 1167, 1457, 2731
SmlI	CTYRAG	8 cut(s)	927, 1285, 1820, 2008, 2891, 3125, 3236, 3284
SmoI	CTYRAG	8 cut(s)	927, 1285, 1820, 2008, 2891, 3125, 3236, 3284
SphI	GCATGC	2 cut(s)	763, 2214
SsiI	CCGC	5 cut(s)	192, 418, 479, 1327, 1436
SspI	AATATT	5 cut(s)	124, 264, 412, 1783, 2468
SspMI	CTAG	4 cut(s)	1839, 2502, 2606, 3340
StyD4I	CCNGG	9 cut(s)	106, 720, 915, 1269, 1297, 1656, 1684, 2114, 2538
StyI	CCWWGG	3 cut(s)	471, 1055, 3049
TaaI	ACNGT	3 cut(s)	667, 2659, 3095
TaiI	ACGT	4 cut(s)	260, 1390, 2279, 2788
TaqI	TCGA	6 cut(s)	80, 808, 1252, 3097, 3126, 3321
TaqII	GACCGA	1 cut(s)	1861
TatI	WGTACW	2 cut(s)	1998, 3129
TauI	GCSGC	1 cut(s)	421
Tru1I	TTAA	9 cut(s)	426, 485, 510, 531, 815, 846, 1845, 2675, 2865
Tru9I	TTAA	9 cut(s)	426, 485, 510, 531, 815, 846, 1845, 2675, 2865
TscAI	CASTG	9 cut(s)	167, 250, 1003, 1161, 1366, 1612, 1714, 2733, 2885
TseFI	GTSAC	3 cut(s)	1603, 2724, 2876
TseI	GCWGC	7 cut(s)	97, 763, 1015, 1548, 1814, 2207, 2315
Tsp45I	GTSAC	3 cut(s)	1603, 2724, 2876
TspGWI	ACGGA	2 cut(s)	1681, 2981
TspRI	CASTG	9 cut(s)	167, 250, 1003, 1161, 1366, 1612, 1714, 2733, 2885
Tth111I	GACNNNGTC	2 cut(s)	1271, 1871
Van91I	CCANNNNNTGG	3 cut(s)	547, 1700, 2355
VneI	GTGCAC	1 cut(s)	591
VpaK11BI	GGWCC	2 cut(s)	695, 913
VspI	ATTAAT	1 cut(s)	426
XagI	CCTNNNNNAGG	2 cut(s)	1559, 2403
XceI	RCATGY	3 cut(s)	763, 2214, 2221
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3192
XhoI	CTCGAG	1 cut(s)	3125
XmiI	GTMKAC	1 cut(s)	3336
XmnI	GAANNNNTTC	3 cut(s)	281, 1614, 2955
XspI	CTAG	4 cut(s)	1839, 2502, 2606, 3340
Zsp2I	ATGCAT	4 cut(s)	46, 1960, 2176, 2187

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.