Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0002601

Physical Location & Seq

Genomic Coordinates Reverse (-)

18037930 .. 18053665

Gene Structure 15736 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00346940.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4455 bp

ATGGAAGTGGATCCGGAATCGGTAGGCATGGAAGTGCCAATCCTCGGCAGCGATTCAGTGAAATGGATCGAGGTCCCTATTCTTTCATCTAATGAATTGGCTGCCACCGCCGATGCTGATACTTGTGCCCCTCTCACTCACGATTGCGCTTCTTCCATCGCCATTGGAGACCCTCCTACATATCTCATATGGAGAATCCACAAGGATTTCCCCCGTACGCTCGAATTACTCGAGCTCTCTGCTAAGAAAGAATTTCCAAAAGTCGGACTGCGAATCACTTTTCCAGAAGCACTCTCTTCTTCAGTTTTTCTTTGCAAAAATGAGATTGATATCGGTTCCAGGAGCCACCCCTATTTGCTTTATGCATTGACTGTCGCCGGAGTCGCTTACCTTTTGAAACTTGGGACCACCTCTAGCTATGCACCCAGCTCTGTTATTAGAGAGTTTAACTTGCATCCCTACGGACCTATTAGCAGTGCCGCGGCAACGCCGACCGGATGTCTTGTTGTAGGTCGGAATGACGGCTCACTTGCTTGCTTTCAACTCACACTTGAATCCACTGCTCCTGGTTTTCTGCAAGAGCTACGGGATGATTCAGGAACTGGTAGTTTCTGGGGTTTCATGTCAAGGGGTAGGACGGTAGGGGCTGTGCAGGACTTGGTTATATCTATGGTTCATGGGAAACCACTTATATTTGTGATTCATATGGATTGGGTATTAAGAGTATGGGATCTTTCATTTCATGGCAGGCTCTTTGCCAATAAGATGAAAGGTCCAACAGTGGCAGGAGCTGCATCGGTAAGGTTGTGGGTTGGTCAAGCTGTCAATGACTCTAGCATAATTCCTGTGGCAATCTTGTACAGGCATAATTCGGATATTAGCTCAGAGACAATCTACGTATATAGGCTCCATTGTAACGTGAGAGACAGAATTGAATTATTGATATATCGTTCAAAACAAATAATTCCTATTGAGGAGGATGGATGGATTGATGTCAAACTGATTTCCAACAAGATATGCATATTGAAAAATGACGGACTGGTGTTTCTCAAGTTGTTGCATATAAATGCCGAGACGGTAGATGCAGTTTGTTATCCTTTGCAAGAGGACTTTGTTGCAGATCAACTCTTTCAAAGTTCAGAGCATTCTTCGGATGACCTTCTTTTGATAACTCATTCAATATTTTCATCTTCAAGGGATCATATTCTACCCGTTGTATCTTCTATTTTCTTACGTAGGCTGCTTCTTCCTGGCATTCATCATATTGCTGCTTTGCGCACAACTTTGTTGGATTATAATAGGCACTGGACTGAGTCAGATTTCCACTCGTTAACTGCTGATGGGTTGAAGAAGGAAATTCTTTCCCTGATTGAACATGAGGTTCTGACTGGTAATTCATCGTCAATATTTTGCTGCTGGAAAATTTTTTGTGCTCGCTATTTCCAAAACTGGTGCAAGAGCAATGCACCATGTGGCCTGCTTGTTGATTCCTCAACCGGCACTGTTGGTTTAATTAGAAAGAGTTCAGTATCTTTTTTTCGTTCTTTGGAGGATATTGAGCGGGTTAATGATGGCTCTTTGGATGAACTTGGCAATATTCCAAGCTTTGGGTTGGATTCATTTGGCAAGGCTGATTGTAAGCTACTTGCTGAAATGCTCAGGTGTGTCACTAAACTCAGCCAGCAATTGGGAAAAACAGCTGCTGCTATATTTTACGAATTACTCATTACTGCACCACCAGTTATCTCATCTAAAGAAATTATTCCACGCCTATTGAAGATATTAGAAACTGGATATAGTTCAACAGCAGCAGCGCTTCTGATAGCAGATCTTGGAACTGATCTTGCCTGGAAGAAAAATCTAGCAGATCACAAAAAATTGAGGAAATTTTCTATCGACATGATGAAGTCTCTTCATGCTTTGCACGAGAAATCTGGCACATGGAGCCGTATTTTAAGTGTGATTGAGAAGTATCTTAAGTATCTCATACCTCGAAAAATTATACAAAAATTTGATGCTGAAGTAGCATTGGATATTAATGCCTCTATCTTGGTCCCGGCTACGTTTCAAGTTGCAAAGGTTATGTTTGAGTCTGCATTGGATATTCATCTGTTTCTAAGCTATCTAGTGAGCATTAGCAGGCAGATCAATATGCTACATGATGATATATCCAAAATACAACTTGAGTTGGTGCCAATGATTCAAGAAGTCATTTATGAGTGGCTTCTCCTGCATTTCTTTGCCACCACACCATCTGAATCAGCTGCAATTGAAGATTTCAGCTCTCAACTTTCATTGTTACAAATTGATAGCAACACTGGTAGAAGATTATGGAATGAGAAGCTTGGGAAGTGCGAGTTCACTCTGGCTTTTATATTCTTACTTAATGTTAGAAGTTCCTCCAAAGACCAGAACCACTTTTCTCCAAGAAGCATACCAAGTGTTGAGGACATAATTGATTCAGTACGAGAGTTTGATAGCTGGACCATGTGGGGCCAGGATGGGGAATCCCTTACTGTCCTGAAGCGTGCAGTGGATCTTGCATTGATCCTGCTAAGGCACGGGCAGTATGATGCTGTTGAGCACTTACTCACTATTGTGGAGGCACATTTAGAAAAGGAAGGTGGATGGTGCATACTTCATCATCTTTTAGGATGCTGCTTCCTTGCTCAGGCACATCGTGGAGTACATGGGGTTCTGAAAGAGAGGAAAGTTCATGAAGCTGTTCGTTGTTTCTTCAGAGCCGCATCTGGAAACGGGTCAGCTCATGCTTTACAAAGCTTGCCTCAGGAAGCGTGGTTGCCACATCTTGGTATCAATGGTAGCGTATCAGATGCTGCATGGAGGCTCCATTACTATCAGTGGGCAATGCAGATATGTGAGCATTATAATATTAGTGAAGGTGCATGTCAGTTTGCTGTTGCTGCGCTTGAGCAAGTTGAGGAAGCTTACAGTGCAAACAATGAGTCCCATGACAGAGCTCCATTTAATGATTCAGTATCTACCATCAAAGGAAGACTTTGGGCAAATGTTTGCAAATTCACTTTAGATCTCAATCTCTATTATGATGCATATTGTGCAATAATTTCAAATCCAGATGAGGAGAGCAAGTACATCTGCTTGAGGCGTCTTATAAATGTTCTTTATGAACGTGGTGCCATAAAGATTCTATGTGGTGGTCAGCTACCATTCATTGGTCTTACAGAGAAGGTAGAGCAAGAGCTTGCATGGAAGGCAGAGCGGTCAGACATTTTAGCAAAGACAAACTTATATAAGTTGCTTTATGCATTTGAAATGCATCAGCATAACTGGCGAAAAGCAGCAAGCTACATGTACCTTTATTCTGCCCGTTTGAGAACTGAAGCATCGCTGAAAGGTTACCAACAATTGTGGCGAGTACTGAAGGAGATACTGAATGGGCTCTCTGCTGCTATCAACGCACTTCATCTTGTTCATCCAGCATATGCTTGGATTGATCCTCCGCTTGCGAGAAGTGCTCTTCACAACGAGCAGTATCCAGGTAGAGAGGCTAGAATAACAATTGAAGACCAGCCAGCAAACAATGATGTTCATCCTCGAAGTTGGAAATCTTTTATGGATATTCAGAAAATTGAGAACGAGTTTGTGCTTACTTCAGCAGAACATCTGCTTTCACTGGCACATGTCAAATGGACAAATACAGGAACCCAGACAGCACCATTAGAGTTGGTTGATCTTTTGATCCAGACAAATCTATATGATATGGCATTTACAGTTCTTTTAAGATTCTTCAAAGGTTCAGAGTTAAAGAGGGGATTGGAAAGAGTTTTCTCAGCTATGTCACTAAAATGCTTTCCACATATAGTTGATTCTTCAAGGGTTGGGGATGATCCCATCGTTCATGGTTCACCAGATATGTCTTCAGCAAATCAACAATCCAATGGAATAAATCAATGGGGAACTCTTGCGCTTTATCTTGACCGGTATAAAGTGTTCCATGCACGGCTACCACTAATTGTTGCCGAAACTCTTCTTTGTACAGATTCTCAAATCCAACTGCCTCTGTGGTTGGTTAAATTATTCAAGGATGGCAGGAGGGAAAAGACATTGGGGATGACTGGCCACGAATCAAATCCGGCATCGTTGTTTCAATTATATGTTGATTATGGTCGACATGGAGAAGCTATAAATCTGTTGCTCGAGCACAAAGGGTGTTTTGGATCCATGAGACCAGCCAACATCATGAATCGTAAAAGACCATTTGGAGTTTGGTTTCCGTATACAATGATTCAGCGCCTCTGGTGCCAACTTGAGGAAATGATAAATTCCGGTCACATGGTAGATCAATGTAAGCAGTTCAAAGATTTGCTACACAGAGCTTTGCTGAAACATCTCCAACTGGTAAAGATGGACTCGGAAGAAGCACTTAGTTTTATTTCAGAAAATAAGATGGAAACACTTCGGATGCAATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0008104 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016973 GO:0031090 GO:0031503 GO:0031965 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0034613 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

DUF5311 Nup160

Protein Analysis

1484

Amino Acids

167.33

Weight (kDa)

6.34

Isoelectric Point (pI)

42.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_Nup120_160	PF11715	61 - 520	7.9e-55	Nucleoporin Nup120/160, beta-propeller domain
NUP160_helical_2	PF17238	543 - 730	1.3e-82	NUP160, helical domain
TPR_NUP160_120_M	PF23354	899 - 1150	1e-34	NUP160/120 middle TPR
HEAT_Nup120	PF23300	900 - 1050	1.5e-12	Nucleoporin Nup120, HEAT repeats
TPR_Nup160_C	PF23347	1202 - 1452	2.1e-08	Nup160 C-terminal TPR

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002601)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33410 AT1G33410
fragaria_vesca	FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400
malus_domestica	MD00G1034800.v1.1 MD05G1063300.v1.1 MD05G1161100.v1.1 MD05G1162300.v1.1 MD05G1162400.v1.1 MD07G1280900.v1.1 MD08G1158600.v1.1 MD10G1009800.v1.1 MD10G1160000.v1.1
prunus_persica	Prupe.5G022000_v2.0.a1
pyrus_communis	pycom10g00600
rosa_chinensis	RchiOBHm_Chr2g0126921 RchiOBHm_Chr4g0402211
rosa_laevigata	RLG00000009106 RLG00000018928
rosa_multiflora	Rmu_sc0000007.1_g000030 Rmu_sc0001405.1_g000051
rosa_roxburghii	Rroxscaffold_1G00047890 Rroxscaffold_2G00116920 Rroxscaffold_5G00346940
rosa_rugosa	Rorug02G0270100 Rorug02G0270200
rosa_samantha	Rh2AG322500 Rh2BG330700 Rh2CG310600 Rh2DG350000 Rh4BG104100 Rh4CG117900 Rh4DG101500
rosa_wichuraiana	Rw2G026160 Rw4G008910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1296, 2889, 3134
Acc16I	TGCGCA	1 cut(s)	1277
AccB1I	GGYRCC	3 cut(s)	2191, 3155, 4281
AccB7I	CCANNNNNTGG	4 cut(s)	1607, 1687, 2810, 3194
AccBSI	CCGCTC	2 cut(s)	1561, 3241
AccI	GTMKAC	2 cut(s)	4150, 4259
AccII	CGCG	1 cut(s)	482
AccIII	TCCGGA	1 cut(s)	13
AciI	CCGC	7 cut(s)	108, 480, 482, 1561, 2745, 3241, 3482
AcoI	YGGCCR	1 cut(s)	4099
AcsI	RAATTY	7 cut(s)	251, 1356, 1422, 1886, 2009, 3038, 4303
AcuI	CTGAAG	8 cut(s)	285, 2040, 2543, 2722, 3381, 3422, 3618, 3885
AcyI	GRCGYC	1 cut(s)	3127
AdeI	CACNNNGTG	2 cut(s)	1472, 2681
AfaI	GTAC	8 cut(s)	217, 860, 2466, 2688, 3113, 3335, 3399, 4018
AfeI	AGCGCT	1 cut(s)	1815
AfiI	CCNNNNNNNGG	9 cut(s)	44, 263, 1607, 1687, 2503, 2671, 2810, 3194, 4291
AflII	CTTAAG	1 cut(s)	1976
AflIII	ACRYGT	2 cut(s)	3330, 3661
AgeI	ACCGGT	1 cut(s)	3960
AhdI	GACNNNNNGTC	1 cut(s)	498
AjnI	CCWGG	6 cut(s)	338, 565, 1249, 1847, 2496, 3517
AleI	CACNNNNGTG	1 cut(s)	2597
Alw21I	GWGCWC	6 cut(s)	237, 1435, 2586, 2983, 3499, 4185
Alw26I	GTCTC	6 cut(s)	162, 881, 918, 1067, 1914, 4201
AlwNI	CAGNNNCTG	4 cut(s)	602, 791, 1703, 2837
Ama87I	CYCGRG	2 cut(s)	230, 4178
Aor13HI	TCCGGA	1 cut(s)	13
Aor51HI	AGCGCT	1 cut(s)	1815
AoxI	GGCC	3 cut(s)	1474, 2494, 4099
ApoI	RAATTY	7 cut(s)	251, 1356, 1422, 1886, 2009, 3038, 4303
ArsI	GACNNNNNNTTYG	2 cut(s)	2951, 2983
AseI	ATTAAT	1 cut(s)	2037
AsiGI	ACCGGT	1 cut(s)	3960
Asp700I	GAANNNNTTC	5 cut(s)	1522, 1761, 2724, 4008, 4437
AspLEI	GCGC	6 cut(s)	149, 1278, 1816, 2929, 3949, 4275
AspS9I	GGNCC	7 cut(s)	73, 405, 464, 773, 2053, 2484, 2494
AsuC2I	CCSGG	1 cut(s)	2057
AsuHPI	GGTGA	1 cut(s)	3879
AvaI	CYCGRG	2 cut(s)	230, 4178
AvaII	GGWCC	6 cut(s)	73, 405, 464, 773, 2053, 2484
AxyI	CCTNAGG	1 cut(s)	2787
BaeGI	GKGCMC	1 cut(s)	130
BalI	TGGCCA	1 cut(s)	4101
BamHI	GGATCC	2 cut(s)	10, 4199
BanI	GGYRCC	3 cut(s)	2191, 3155, 4281
BanII	GRGCYC	3 cut(s)	237, 2983, 3423
BauI	CACGAG	1 cut(s)	1925
BbsI	GAAGAC	3 cut(s)	3022, 3552, 3891
Bbv12I	GWGCWC	6 cut(s)	237, 1435, 2586, 2983, 3499, 4185
BceAI	ACGGC	3 cut(s)	538, 1932, 3998
BcgI	CGANNNNNNTGC	2 cut(s)	221, 255
BciT130I	CCWGG	6 cut(s)	340, 567, 1251, 1849, 2498, 3519
BciVI	GTATCC	1 cut(s)	3525
BcnI	CCSGG	1 cut(s)	2057
BcoDI	GTCTC	6 cut(s)	162, 881, 918, 1067, 1914, 4201
BfaI	CTAG	5 cut(s)	414, 834, 1862, 2126, 3531
BfoI	RGCGCY	2 cut(s)	1817, 4276
BfrI	CTTAAG	1 cut(s)	1976
BfuI	GTATCC	1 cut(s)	3525
BglII	AGATCT	2 cut(s)	1828, 3049
BmcAI	AGTACT	1 cut(s)	3399
Bme1390I	CCNGG	7 cut(s)	340, 567, 1251, 1849, 2057, 2498, 3519
Bme18I	GGWCC	6 cut(s)	73, 405, 464, 773, 2053, 2484
BmeRI	GACNNNNNGTC	1 cut(s)	498
BmeT110I	CYCGRG	2 cut(s)	230, 4178
BmgT120I	GGNCC	7 cut(s)	73, 405, 464, 773, 2053, 2484, 2494
BmrFI	CCNGG	7 cut(s)	340, 567, 1251, 1849, 2057, 2498, 3519
BpiI	GAAGAC	3 cut(s)	3022, 3552, 3891
BplI	GAGNNNNNCTC	2 cut(s)	3481, 3513
Bpu10I	CCTNAGC	3 cut(s)	1658, 2555, 2670
BpuEI	CTTGAG	5 cut(s)	1034, 2204, 2951, 3142, 4310
BpuMI	CCSGG	1 cut(s)	2057
BsaAI	YACGTR	2 cut(s)	898, 1235
BsaBI	GATNNNNATC	4 cut(s)	329, 2106, 3054, 3570
BsaHI	GRCGYC	1 cut(s)	3127
BsaI	GGTCTC	2 cut(s)	162, 4201
BsaJI	CCNNGG	2 cut(s)	43, 480
BsaWI	WCCGGW	4 cut(s)	13, 494, 3960, 4307
BsaXI	ACNNNNNCTCC	2 cut(s)	3095, 3125
Bsc4I	CCNNNNNNNGG	9 cut(s)	44, 263, 1607, 1687, 2503, 2671, 2810, 3194, 4291
Bse118I	RCCGGY	2 cut(s)	1496, 3960
Bse21I	CCTNAGG	1 cut(s)	2787
Bse3DI	GCAATG	2 cut(s)	1468, 2874
Bse8I	GATNNNNATC	4 cut(s)	329, 2106, 3054, 3570
BseAI	TCCGGA	1 cut(s)	13
BseBI	CCWGG	6 cut(s)	340, 567, 1251, 1849, 2498, 3519
BseDI	CCNNGG	2 cut(s)	43, 480
BseJI	GATNNNNATC	4 cut(s)	329, 2106, 3054, 3570
BseLI	CCNNNNNNNGG	9 cut(s)	44, 263, 1607, 1687, 2503, 2671, 2810, 3194, 4291
BseMI	GCAATG	2 cut(s)	1468, 2874
BseMII	CTCAG	7 cut(s)	897, 1302, 1672, 1690, 2684, 2801, 3825
BseRI	GAGGAG	2 cut(s)	989, 3116
BseSI	GKGCMC	1 cut(s)	130
BseYI	CCCAGC	1 cut(s)	425
BsgI	GTGCAG	3 cut(s)	671, 1716, 2550
Bsh1236I	CGCG	1 cut(s)	482
Bsh1285I	CGRYCG	1 cut(s)	495
BshFI	GGCC	3 cut(s)	1476, 2496, 4101
BshNI	GGYRCC	3 cut(s)	2191, 3155, 4281
BshTI	ACCGGT	1 cut(s)	3960
BsiEI	CGRYCG	1 cut(s)	495
BsiHKAI	GWGCWC	6 cut(s)	237, 1435, 2586, 2983, 3499, 4185
BsiHKCI	CYCGRG	2 cut(s)	230, 4178
BsiSI	CCGG	8 cut(s)	14, 378, 495, 1497, 2057, 3961, 4115, 4308
BsiWI	CGTACG	1 cut(s)	215
BslFI	GGGAC	4 cut(s)	59, 418, 2039, 2953
BslI	CCNNNNNNNGG	9 cut(s)	44, 263, 1607, 1687, 2503, 2671, 2810, 3194, 4291
BsmAI	GTCTC	6 cut(s)	162, 881, 918, 1067, 1914, 4201
BsmBI	CGTCTC	1 cut(s)	1067
BsmFI	GGGAC	4 cut(s)	59, 418, 2039, 2953
BsmI	GAATGC	2 cut(s)	1144, 1254
BsnI	GGCC	3 cut(s)	1476, 2496, 4101
Bso31I	GGTCTC	2 cut(s)	162, 4201
BsoBI	CYCGRG	2 cut(s)	230, 4178
Bsp1286I	GDGCHC	8 cut(s)	130, 237, 1435, 2586, 2983, 3423, 3499, 4185
Bsp13I	TCCGGA	1 cut(s)	13
Bsp1407I	TGTACA	2 cut(s)	858, 4016
BspACI	CCGC	7 cut(s)	108, 480, 482, 1561, 2745, 3241, 3482
BspANI	GGCC	3 cut(s)	1476, 2496, 4101
BspCNI	CTCAG	7 cut(s)	896, 1303, 1671, 1689, 2683, 2800, 3824
BspEI	TCCGGA	1 cut(s)	13
BspFNI	CGCG	1 cut(s)	482
BspHI	TCATGA	2 cut(s)	2716, 4221
BspQI	GCTCTTC	1 cut(s)	3504
BspT107I	GGYRCC	3 cut(s)	2191, 3155, 4281
BspTI	CTTAAG	1 cut(s)	1976
BspTNI	GGTCTC	2 cut(s)	162, 4201
BsrBI	CCGCTC	2 cut(s)	1561, 3241
BsrDI	GCAATG	2 cut(s)	1468, 2874
BsrFI	RCCGGY	2 cut(s)	1496, 3960
BsrGI	TGTACA	2 cut(s)	858, 4016
BssAI	RCCGGY	2 cut(s)	1496, 3960
BssECI	CCNNGG	2 cut(s)	43, 480
BssNAI	GTATAC	1 cut(s)	4260
BssNI	GRCGYC	1 cut(s)	3127
BssSI	CACGAG	1 cut(s)	1925
Bst1107I	GTATAC	1 cut(s)	4260
Bst2BI	CACGAG	1 cut(s)	1925
Bst2UI	CCWGG	6 cut(s)	340, 567, 1251, 1849, 2498, 3519
Bst4CI	ACNGT	8 cut(s)	373, 640, 781, 1078, 1504, 2518, 2954, 3754
Bst6I	CTCTTC	4 cut(s)	301, 1917, 3504, 4014
BstACI	GRCGYC	1 cut(s)	3127
BstAFI	CTTAAG	1 cut(s)	1976
BstAPI	GCANNNNNTGC	2 cut(s)	791, 3077
BstAUI	TGTACA	2 cut(s)	858, 4016
BstBAI	YACGTR	2 cut(s)	898, 1235
BstDSI	CCRYGG	1 cut(s)	480
BstEII	GGTNACC	1 cut(s)	3377
BstENI	CCTNNNNNAGG	1 cut(s)	2669
BstFNI	CGCG	1 cut(s)	482
BstH2I	RGCGCY	2 cut(s)	1817, 4276
BstHHI	GCGC	6 cut(s)	149, 1278, 1816, 2929, 3949, 4275
BstMAI	GTCTC	6 cut(s)	162, 881, 918, 1067, 1914, 4201
BstMCI	CGRYCG	1 cut(s)	495
BstNI	CCWGG	6 cut(s)	340, 567, 1251, 1849, 2498, 3519
BstNSI	RCATGY	3 cut(s)	2910, 3334, 3665
BstPI	GGTNACC	1 cut(s)	3377
BstSCI	CCNGG	7 cut(s)	338, 565, 1249, 1847, 2055, 2496, 3517
BstSLI	GKGCMC	1 cut(s)	130
BstSNI	TACGTA	2 cut(s)	898, 1235
BstUI	CGCG	1 cut(s)	482
BstV2I	GAAGAC	3 cut(s)	3022, 3552, 3891
BstX2I	RGATCY	6 cut(s)	10, 730, 1828, 2536, 3049, 4199
BstYI	RGATCY	6 cut(s)	10, 730, 1828, 2536, 3049, 4199
BstZ17I	GTATAC	1 cut(s)	4260
Bsu36I	CCTNAGG	1 cut(s)	2787
BsuI	GTATCC	1 cut(s)	3525
BsuRI	GGCC	3 cut(s)	1476, 2496, 4101
BtgI	CCRYGG	1 cut(s)	480
BtgZI	GCGATG	2 cut(s)	142, 3351
BtsI	GCAGTG	3 cut(s)	481, 558, 2538
CaiI	CAGNNNCTG	4 cut(s)	602, 791, 1703, 2837
CciI	TCATGA	2 cut(s)	2716, 4221
CfoI	GCGC	6 cut(s)	149, 1278, 1816, 2929, 3949, 4275
Cfr10I	RCCGGY	2 cut(s)	1496, 3960
Cfr13I	GGNCC	7 cut(s)	73, 405, 464, 773, 2053, 2484, 2494
Cfr42I	CCGCGG	1 cut(s)	483
CseI	GACGC	1 cut(s)	3116
Csp6I	GTAC	8 cut(s)	216, 859, 2465, 2687, 3112, 3334, 3398, 4017
CspAI	ACCGGT	1 cut(s)	3960
CspCI	CAANNNNNGTGG	4 cut(s)	1755, 1790, 3371, 3406
CviQI	GTAC	8 cut(s)	216, 859, 2465, 2687, 3112, 3334, 3398, 4017
DraIII	CACNNNGTG	2 cut(s)	1472, 2681
DriI	GACNNNNNGTC	1 cut(s)	498
EaeI	YGGCCR	1 cut(s)	4099
Eam1104I	CTCTTC	4 cut(s)	301, 1917, 3504, 4014
Eam1105I	GACNNNNNGTC	1 cut(s)	498
EarI	CTCTTC	4 cut(s)	301, 1917, 3504, 4014
Ecl136II	GAGCTC	2 cut(s)	235, 2981
Eco105I	TACGTA	2 cut(s)	898, 1235
Eco24I	GRGCYC	3 cut(s)	237, 2983, 3423
Eco31I	GGTCTC	2 cut(s)	162, 4201
Eco32I	GATATC	1 cut(s)	331
Eco47I	GGWCC	6 cut(s)	73, 405, 464, 773, 2053, 2484
Eco47III	AGCGCT	1 cut(s)	1815
Eco53kI	GAGCTC	2 cut(s)	235, 2981
Eco57I	CTGAAG	8 cut(s)	285, 2040, 2543, 2722, 3381, 3422, 3618, 3885
Eco81I	CCTNAGG	1 cut(s)	2787
Eco88I	CYCGRG	2 cut(s)	230, 4178
Eco91I	GGTNACC	1 cut(s)	3377
EcoICRI	GAGCTC	2 cut(s)	235, 2981
EcoNI	CCTNNNNNAGG	1 cut(s)	2669
EcoO109I	RGGNCCY	1 cut(s)	73
EcoO65I	GGTNACC	1 cut(s)	3377
EcoRII	CCWGG	6 cut(s)	338, 565, 1249, 1847, 2496, 3517
EcoRV	GATATC	1 cut(s)	331
EcoT22I	ATGCAT	5 cut(s)	367, 1022, 3073, 3289, 3300
EcoT38I	GRGCYC	3 cut(s)	237, 2983, 3423
Esp3I	CGTCTC	1 cut(s)	1067
FalI	AAGNNNNNCTT	6 cut(s)	3003, 3035, 3251, 3283, 4389, 4421
FaqI	GGGAC	4 cut(s)	59, 418, 2039, 2953
FauI	CCCGC	1 cut(s)	1554
FauNDI	CATATG	3 cut(s)	188, 705, 3463
FblI	GTMKAC	2 cut(s)	4150, 4259
FriOI	GRGCYC	3 cut(s)	237, 2983, 3423
FspBI	CTAG	5 cut(s)	414, 834, 1862, 2126, 3531
FspI	TGCGCA	1 cut(s)	1277
GlaI	GCGC	6 cut(s)	148, 1277, 1815, 2928, 3948, 4274
GsaI	CCCAGC	1 cut(s)	429
HaeII	RGCGCY	2 cut(s)	1817, 4276
HaeIII	GGCC	3 cut(s)	1476, 2496, 4101
HapII	CCGG	8 cut(s)	14, 378, 495, 1497, 2057, 3961, 4115, 4308
HgaI	GACGC	1 cut(s)	3116
HhaI	GCGC	6 cut(s)	149, 1278, 1816, 2929, 3949, 4275
Hin1I	GRCGYC	1 cut(s)	3127
Hin6I	GCGC	6 cut(s)	147, 1276, 1814, 2927, 3947, 4273
HinP1I	GCGC	6 cut(s)	147, 1276, 1814, 2927, 3947, 4273
HincII	GTYRAC	2 cut(s)	1332, 4151
HindII	GTYRAC	2 cut(s)	1332, 4151
HindIII	AAGCTT	4 cut(s)	1603, 2342, 2779, 2946
HpaI	GTTAAC	1 cut(s)	1332
HpaII	CCGG	8 cut(s)	14, 378, 495, 1497, 2057, 3961, 4115, 4308
HphI	GGTGA	1 cut(s)	3879
Hpy166II	GTNNAC	5 cut(s)	1332, 2361, 3887, 4151, 4260
Hpy8I	GTNNAC	5 cut(s)	1332, 2361, 3887, 4151, 4260
HpyAV	CCTTC	7 cut(s)	1169, 1345, 2615, 2894, 3202, 3226, 3397
HpyCH4III	ACNGT	8 cut(s)	373, 640, 781, 1078, 1504, 2518, 2954, 3754
HpyCH4IV	ACGT	5 cut(s)	897, 918, 1234, 2063, 3151
HpySE526I	ACGT	5 cut(s)	897, 918, 1234, 2063, 3151
Hsp92I	GRCGYC	1 cut(s)	3127
HspAI	GCGC	6 cut(s)	147, 1276, 1814, 2927, 3947, 4273
Kpn2I	TCCGGA	1 cut(s)	13
KspAI	GTTAAC	1 cut(s)	1332
KspI	CCGCGG	1 cut(s)	483
LguI	GCTCTTC	1 cut(s)	3504
LmnI	GCTCC	7 cut(s)	342, 568, 788, 912, 1944, 2853, 2986
MaeI	CTAG	5 cut(s)	414, 834, 1862, 2126, 3531
MaeII	ACGT	5 cut(s)	897, 918, 1234, 2063, 3151
MaeIII	GTNAC	6 cut(s)	914, 1666, 2298, 3377, 3819, 4310
MbiI	CCGCTC	2 cut(s)	1561, 3241
MfeI	CAATTG	4 cut(s)	1685, 2268, 3386, 3540
MflI	RGATCY	6 cut(s)	10, 730, 1828, 2536, 3049, 4199
MhlI	GDGCHC	8 cut(s)	130, 237, 1435, 2586, 2983, 3423, 3499, 4185
MlsI	TGGCCA	1 cut(s)	4101
MluNI	TGGCCA	1 cut(s)	4101
MlyI	GAGTC	6 cut(s)	390, 824, 1322, 2099, 2975, 4385
MmeI	TCCRAC	9 cut(s)	244, 494, 800, 1032, 1269, 1593, 3563, 4057, 4399
Mox20I	TGGCCA	1 cut(s)	4101
Mph1103I	ATGCAT	5 cut(s)	367, 1022, 3073, 3289, 3300
MroI	TCCGGA	1 cut(s)	13
MroXI	GAANNNNTTC	5 cut(s)	1522, 1761, 2724, 4008, 4437
MscI	TGGCCA	1 cut(s)	4101
MslI	CAYNNNNRTG	4 cut(s)	32, 1065, 2597, 3826
Msp20I	TGGCCA	1 cut(s)	4101
MspA1I	CMGCKG	3 cut(s)	482, 1700, 2264
MspCI	CTTAAG	1 cut(s)	1976
MspI	CCGG	8 cut(s)	14, 378, 495, 1497, 2057, 3961, 4115, 4308
MspR9I	CCNGG	7 cut(s)	340, 567, 1251, 1849, 2057, 2498, 3519
MunI	CAATTG	4 cut(s)	1685, 2268, 3386, 3540
Mva1269I	GAATGC	2 cut(s)	1144, 1254
MvaI	CCWGG	6 cut(s)	340, 567, 1251, 1849, 2498, 3519
MvnI	CGCG	1 cut(s)	482
NciI	CCSGG	1 cut(s)	2057
NdeI	CATATG	3 cut(s)	188, 705, 3463
NmeAIII	GCCGAG	2 cut(s)	24, 1096
NmuCI	GTSAC	3 cut(s)	1666, 3819, 4310
NsbI	TGCGCA	1 cut(s)	1277
NsiI	ATGCAT	5 cut(s)	367, 1022, 3073, 3289, 3300
NspI	RCATGY	3 cut(s)	2910, 3334, 3665
OliI	CACNNNNGTG	1 cut(s)	2597
PaeR7I	CTCGAG	2 cut(s)	230, 4178
PagI	TCATGA	2 cut(s)	2716, 4221
PciI	ACATGT	2 cut(s)	3330, 3661
PciSI	GCTCTTC	1 cut(s)	3504
PcsI	WCGNNNNNNNCGW	1 cut(s)	228
PctI	GAATGC	2 cut(s)	1144, 1254
PdmI	GAANNNNTTC	5 cut(s)	1522, 1761, 2724, 4008, 4437
Pfl23II	CGTACG	1 cut(s)	215
PflFI	GACNNNGTC	1 cut(s)	1312
PflMI	CCANNNNNTGG	4 cut(s)	1607, 1687, 2810, 3194
PfoI	TCCNGGA	1 cut(s)	338
PinAI	ACCGGT	1 cut(s)	3960
PleI	GAGTC	6 cut(s)	389, 824, 1321, 2098, 2974, 4385
PpsI	GAGTC	6 cut(s)	389, 824, 1321, 2098, 2974, 4385
Ppu21I	YACGTR	2 cut(s)	898, 1235
PpuMI	RGGWCCY	1 cut(s)	73
PscI	ACATGT	2 cut(s)	3330, 3661
PshBI	ATTAAT	1 cut(s)	2037
PsiI	TTATAA	3 cut(s)	1296, 2889, 3134
Psp124BI	GAGCTC	2 cut(s)	237, 2983
Psp5II	RGGWCCY	1 cut(s)	73
Psp6I	CCWGG	6 cut(s)	338, 565, 1249, 1847, 2496, 3517
PspEI	GGTNACC	1 cut(s)	3377
PspFI	CCCAGC	1 cut(s)	425
PspGI	CCWGG	6 cut(s)	338, 565, 1249, 1847, 2496, 3517
PspLI	CGTACG	1 cut(s)	215
PspPI	GGNCC	7 cut(s)	73, 405, 464, 773, 2053, 2484, 2494
PspPPI	RGGWCCY	1 cut(s)	73
PspXI	VCTCGAGB	2 cut(s)	230, 4178
PsrI	GAACNNNNNNTAC	4 cut(s)	2679, 2711, 3956, 3988
PstNI	CAGNNNCTG	4 cut(s)	602, 791, 1703, 2837
PsuI	RGATCY	6 cut(s)	10, 730, 1828, 2536, 3049, 4199
PsyI	GACNNNGTC	1 cut(s)	1312
PvuII	CAGCTG	2 cut(s)	1700, 2264
RsaI	GTAC	8 cut(s)	217, 860, 2466, 2688, 3113, 3335, 3399, 4018
RsaNI	GTAC	8 cut(s)	216, 859, 2465, 2687, 3112, 3334, 3398, 4017
RseI	CAYNNNNRTG	4 cut(s)	32, 1065, 2597, 3826
SacI	GAGCTC	2 cut(s)	237, 2983
SacII	CCGCGG	1 cut(s)	483
SalI	GTCGAC	1 cut(s)	4149
SapI	GCTCTTC	1 cut(s)	3504
Sau96I	GGNCC	7 cut(s)	73, 405, 464, 773, 2053, 2484, 2494
ScaI	AGTACT	1 cut(s)	3399
SchI	GAGTC	6 cut(s)	390, 824, 1322, 2099, 2975, 4385
ScrFI	CCNGG	7 cut(s)	340, 567, 1251, 1849, 2057, 2498, 3519
SduI	GDGCHC	8 cut(s)	130, 237, 1435, 2586, 2983, 3423, 3499, 4185
Sfr274I	CTCGAG	2 cut(s)	230, 4178
Sfr303I	CCGCGG	1 cut(s)	483
SgrBI	CCGCGG	1 cut(s)	483
SinI	GGWCC	6 cut(s)	73, 405, 464, 773, 2053, 2484
SlaI	CTCGAG	2 cut(s)	230, 4178
SmiMI	CAYNNNNRTG	4 cut(s)	32, 1065, 2597, 3826
SmlI	CTYRAG	8 cut(s)	230, 1049, 1976, 2183, 2930, 3121, 4178, 4289
SmoI	CTYRAG	8 cut(s)	230, 1049, 1976, 2183, 2930, 3121, 4178, 4289
SnaBI	TACGTA	2 cut(s)	898, 1235
SsiI	CCGC	7 cut(s)	108, 480, 482, 1561, 2745, 3241, 3482
SspI	AATATT	4 cut(s)	1182, 1407, 1597, 2893
SspMI	CTAG	5 cut(s)	414, 834, 1862, 2126, 3531
SstI	GAGCTC	2 cut(s)	237, 2983
StyD4I	CCNGG	7 cut(s)	338, 565, 1249, 1847, 2055, 2496, 3517
TaaI	ACNGT	8 cut(s)	373, 640, 781, 1078, 1504, 2518, 2954, 3754
TaiI	ACGT	5 cut(s)	900, 921, 1237, 2066, 3154
TaqI	TCGA	8 cut(s)	69, 222, 231, 1896, 1993, 3577, 4150, 4179
TatI	WGTACW	5 cut(s)	858, 2686, 3111, 3397, 4016
TauI	GCSGC	3 cut(s)	482, 485, 2747
TseFI	GTSAC	3 cut(s)	1666, 3819, 4310
Tsp45I	GTSAC	3 cut(s)	1666, 3819, 4310
TspGWI	ACGGA	3 cut(s)	477, 1050, 4245
Tth111I	GACNNNGTC	1 cut(s)	1312
Van91I	CCANNNNNTGG	4 cut(s)	1607, 1687, 2810, 3194
Vha464I	CTTAAG	1 cut(s)	1976
VpaK11BI	GGWCC	6 cut(s)	73, 405, 464, 773, 2053, 2484
VspI	ATTAAT	1 cut(s)	2037
XagI	CCTNNNNNAGG	1 cut(s)	2669
XapI	RAATTY	7 cut(s)	251, 1356, 1422, 1886, 2009, 3038, 4303
XceI	RCATGY	3 cut(s)	2910, 3334, 3665
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3465, 4245
XhoI	CTCGAG	2 cut(s)	230, 4178
XmiI	GTMKAC	2 cut(s)	4150, 4259
XmnI	GAANNNNTTC	5 cut(s)	1522, 1761, 2724, 4008, 4437
XspI	CTAG	5 cut(s)	414, 834, 1862, 2126, 3531
ZrmI	AGTACT	1 cut(s)	3399
Zsp2I	ATGCAT	5 cut(s)	367, 1022, 3073, 3289, 3300

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.