Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0002601

Physical Location & Seq

Genomic Coordinates Reverse (-)

15446021 .. 15471481

Gene Structure 25461 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG101500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4527 bp

ATGGAAGTGGATCCGGAATCGGTAGGCATGGAAGTGCCAATCCTCGGCAGCGATTCAGTGAAATGGATCGAGTTCTCTATTCTTTCATCTAATGAATTGGCCGCCACCGCCGATGCTGATACTTGTGCCCCTCTCACTCACGATTGCGCTTCTTCCATCGCCATTGGAGACCCTCCTACATATCTCATATGGAGAATCCACAAGGATTTCCCCCGTACTCTCGAATTACTCGAGCTCTCTGCTAAGAAAGAATTTCCAAAAGTCGGACTGCGAATCACTTTTCCAGAAGCACTCTCTTCTTCAGTTTTTCTTTGCAAAAATGAGATTGATATCGGTTCCAGGAGCCACCCCTATTTGCTTTATGCATTGACCGTGGCCGGAGTCGCTTACCTTTTGAAACTTGGGACCACCTCTAGCTATGCACCCAGCTCTGTTATTAGAGAGTTTAACTTGCATCCCTACGGACCAATTAGCAGTGCCGCAGCAACGCTGACCGGATGTCTTGTTGTAGGTCGGAATGACGGCTCACTTGCTTGCTTTCAACTCACACTTGAATCCACTGCTCCTGGTTTTCTGCAAGAGGTACGGGATGATTCAGGAACTGGTAGTTTCTGGGGTTTCAAGTCAAGGGGTAGGACGGTAGGGGCTGTGCAGGACTTGGTTATATCTATGGTTCATGGGAAACCACTTATATTTGTGATTCATACGGATTGGGTGTTAAGAGTATGGGATCTTTCATTTCATGGCAGGCTCTTTGCCAATAAGATGAAAGGTCCAACAGTGGCAGGAGCTGCATCGGTAAGGTTGTGGGTTGGTCAAGCTGACAATGACTCGAGCATAATTCATGTGGCAATCTTGTACAGGCATAATTCGGATATTAGCTCAGAGACAATCTACGTATATAGGCTCCATTGTAATGTGAGAGACAGAATTGAATTATTGATATATCGTTCAAAACAAATAATTCCTATTGAGGAGGATGGATGGATTGATGTCAAACTTATTTCCAACAAGATATGCATATTGAAAAATCACGGACTGGTGTTTCTCAAGTTGTTGCATGTCTCAGCATTTATGGTAGATGCAGTTTGTTATCCTTTGCAAGAGGACTTTGTTGCAGATCAACTCTTTCAAAGTTCAGAGCATTCTTTGGATGACCTTCTTTTGATAACTCATTCAATATTTTCATCTTCAAGGGATCATATTCTACCCTTTGTATCTTCTATTTTCTTACGTAGGCTGCTTCTTCCTGGCATTCATCATAATGCTGCTTTGCGCACAACTTTGTTGGATTATAATAGGCACTGGACTGAGTCAGATTTCCACTCCTTAACTGCTGATGGGTTGAAGAAGGAAATTCTTTCCCTGATTGAACATGAGGTTCTGACTGGTAATTCGTCGTCAATATTTTGCTGCTGGAAAAATTTTTGTGCTCGCTATTTCCAAAACTGGTGCAAGAGCAATGCACCATGTGGCCTGCTTGTTGATTCCTCAACCGGCACTGTTGGTTTAATTAGAAAGAGTTCAGTATCTTTTTTTCGTTCTTTGGAGGATATTGAGCGGGTTAATGATGGCTCTTTGGATGAACTTGGCAATATTCCAAGCTTTGGGTTGGATTCATTTGGCAAGGCTGATTGTAAGCTACTTGCTGAAATGCTTAGGTGTGTCACTAATGTCAGCCAGCAATTGGGAAAAACAGCTTCTGCTATATTTTACGAATTACTCATTAGTGCACCACCAGTTATCTCATCTAAAGAAATTATTCCACGCCTATTGAAGATATTAGAAACTGGATATAGTTCAACAGCAGCAGCGCTTCGGATAGCAGATCTTGGAACTGATCTTGCCTGGGAGAAAAATCTAGCAGATCACAAAAATTTGAGGAAATTTTCTATTGACATGATGAAGTCTCTTCATGCTTTGCATGAGAAATCTGGCACATGGAGCCGTATTTTAAGTGTGATTGAGAAGTATCTTAAGTATCTCATACCTCGAAAAATTATACAAAAATTTGATGCTGAAGTAGCATTGGATATTAATGCCTCCATCTTGGTCCCGGCTACGTTTCAAGTTGCAAAGGTTATGTTTGAGTCTGCATTGGATATTCATCTGTTTCTAAGCTATCTAGTGAGCATTAGCAGGCAGATCAATATGCTACATGATGATATATCCAAAATACAACTTGAGTTGGTGCCAATGATTCAAGAAGTCATTTATGAGTGGCTTCTCCTGCATTTCTTTGCCACCACACCATCTGAATCAGCTGCAATTGAAGATTTCAGCCCTCAACTTTCATTGTTACAAATTGATAGCAACACTGGTAGAAGATTATGGAATGATAAGCTTGGGAAGTGCGAGTTCACTCTGGCTTTTATATTCTTACTTAATGTTAGAAGTTCCTCCAAAGACCAGAACCACTTTTCTCCAAGAAGCATACCAAGCGTTGAGGACATAATTGATTCAGTACGAGAGTTTGATAGCTGGACCATGTGGGGCCAGGATGGGGAATCCCTTACTGTCCTGAAGCGTGCAGTGGATCTTGCATTGATCCTGCTAAGGCACGGGCAGCATGATGCTGTTGAGCACTTACTCACTATTGTGGAGGCACATTTAGAAAAGGAGGAGACTTCTCACGGTATTAGAGCTACAGATGGTGGATGGTGCATACTTCATCATCTTTTAGGATGCTGCTTCCTTGCTCAGGCACATCGTGGAGTACATGGGATTCTGAAAGAGAGGAAAGTTCATGAAGCTGTTCGTTGTTTCTTCAGAGCCGCATCTGGAAACGGGTCATCTCATGCTTTACAAAGCTTGCCTCAGGAAGCGTGGTTGCCACATCTTGGTATCGATGGTAGCGTATCAGATGCTGCATGGAGGCTCCATTACTATCAGTGGGCAATGCAGATATGTGAGCATTATAATATTAGTGAAGGTGCATGTCAGTTTGCTCTTGCTGCGCTTGAGCAAGTTGAGGAAGCTTACAGTGCAAACAATGAGTCCCATGACAGAGCTCCATTTAATGAATCAGTATCTACCATCAAAGGAAGACTTTGGGCAAATGTTTGCAAATTTACTTTAGATCTCAATCTCTATTATGATGCATATTGTGCAATAATTTCAAATCCAGATGAGGAGAGCAAGTACATCTGCTTGAGGCGTCTTATAAATGTTCTTTATGAACGTGGTGCCATAAAGATTCTATGTGGTGGTCAGCTACCATTCATTGGTCTTACAGAGAAGGTAGAACAAGAGCTTGCATGGAAGGCAGAGCGATCAGACATTTTAGCAAAGACAAACTTATATAAGTTGCTTTATGCATTTGAAATGCATCGGCATAACTGGCGAAAAGCAGCAAGCTACATGTACCTTTATTCTGCCCGTTTGAGAACTGAAGCATCTCTGAAAGGTTACCAACAATTGTGGCGAGTACTGAAGGAGATACTGAATGGGCTCTCTGCTGCTATCAACGCACTTCATCTTGTTCATCCAGCATATGCTTGGATTGATCCTCTGCTTGCGAGAAGTGCTCTTCACAACGAGCAGTATCCAGGTAGAGAGGCTAGAATAACAATTGAAGACCAGCCAGCAAACAATGATGTTCATCCTCGAAGTTGGAAATCTTTTATAGATATTCAGAAAATTGAGAACGAGTTTGTGCTTACTTCAGCAGAACATCTGCTTTCACTGGCACATGTTAAATGGACAAATACAGGAACCCAGACAGCACCATTAGAGTTGGTTGATCTTTTGATCCAGACAAATCTATATGATATGGCATTTACAGTTCTTGTAAGATTCTTCAAAGGTTCAGAGTTAAAGAGGGGATTGGAAAGAGTTTTCTCAGCTATGTCACTAAAATGCTTTCCACATATAGTTGATTCTTCAAGGGTTGGGGATGATCCCACAACACAAGGTCTTCTGTTGACTTCTCCAAAGGATGATGTGATCGTTCATGGTTCACCAGATATGTCTTCAGCAAATCAACAATCCAATGGAATAAATCAATGGGGAACTCTCGAGCTTTATCTTGACCGGTATAAAGTGTTCCATGCACGTCTACCACTAATTGTTGCCGAAACTCTTCTTCGTACGGATTCTCAAATCCAACTGCCTCTTTGGTTGGTTAAATTATTCAAGGATGGCAGGAGGGAAAAGACATTGGGGATGACTGGCCACGAATCAAATCCAGCATCGTTGTTTCAATTATATGTTGATTATGGTCGATATGGAGAAGCTATAAATCTGTTGCTCGAGCACAAAGGGTGTTTTGGATCCATGAGACCAGCCAACATCATGAATCGTAAAAGACCATTTGGAGTTTGGTTTCCGTATACAATGATTCAGCGCCTCTGGTGCCAACTTGAGGAAATGATAAATTCCGGTGACATGGTAGATCAATGTAAGCAGTTCAAAGATTTGCTACACAGAACTTTGCTGAAACATCTCCAACTGGTAAAGATGGACTCGGAAGAAGCACTTAGTTTTATTTCAGAAAATAAGATGGAAACACTTCGGATGCAATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0008104 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016973 GO:0031090 GO:0031503 GO:0031965 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0034613 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

DUF5311 Nup160

Protein Analysis

1508

Amino Acids

170.23

Weight (kDa)

6.24

Isoelectric Point (pI)

40.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_Nup120_160	PF11715	61 - 520	2.7e-54	Nucleoporin Nup120/160, beta-propeller domain
NUP160_helical_2	PF17238	543 - 730	4.8e-85	NUP160, helical domain
TPR_NUP160_120_M	PF23354	909 - 1160	3.4e-35	NUP160/120 middle TPR
HEAT_Nup120	PF23300	910 - 1060	1.1e-12	Nucleoporin Nup120, HEAT repeats
TPR_Nup160_C	PF23347	1212 - 1491	2.1e-09	Nup160 C-terminal TPR

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002601)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33410 AT1G33410
fragaria_vesca	FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400
malus_domestica	MD00G1034800.v1.1 MD05G1063300.v1.1 MD05G1161100.v1.1 MD05G1162300.v1.1 MD05G1162400.v1.1 MD07G1280900.v1.1 MD08G1158600.v1.1 MD10G1009800.v1.1 MD10G1160000.v1.1
prunus_persica	Prupe.5G022000_v2.0.a1
pyrus_communis	pycom10g00600
rosa_chinensis	RchiOBHm_Chr2g0126921 RchiOBHm_Chr4g0402211
rosa_laevigata	RLG00000009106 RLG00000018928
rosa_multiflora	Rmu_sc0000007.1_g000030 Rmu_sc0001405.1_g000051
rosa_roxburghii	Rroxscaffold_1G00047890 Rroxscaffold_2G00116920 Rroxscaffold_5G00346940
rosa_rugosa	Rorug02G0270100 Rorug02G0270200
rosa_samantha	Rh2AG322500 Rh2BG330700 Rh2CG310600 Rh2DG350000 Rh4BG104100 Rh4CG117900 Rh4DG101500
rosa_wichuraiana	Rw2G026160 Rw4G008910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1296, 2919, 3164
Acc16I	TGCGCA	1 cut(s)	1277
AccB1I	GGYRCC	3 cut(s)	2191, 3185, 4353
AccB7I	CCANNNNNTGG	4 cut(s)	1607, 1687, 2840, 3224
AccBSI	CCGCTC	1 cut(s)	1561
AccI	GTMKAC	2 cut(s)	4057, 4331
AccIII	TCCGGA	1 cut(s)	13
AciI	CCGC	5 cut(s)	102, 108, 480, 1561, 2775
AcoI	YGGCCR	3 cut(s)	99, 375, 4171
AcsI	RAATTY	8 cut(s)	251, 1356, 1423, 1876, 1886, 2009, 3068, 4375
AcuI	CTGAAG	8 cut(s)	285, 2040, 2543, 2752, 3411, 3452, 3648, 3957
AcyI	GRCGYC	1 cut(s)	3157
AdeI	CACNNNGTG	2 cut(s)	1472, 2711
AfaI	GTAC	9 cut(s)	217, 585, 860, 2466, 2718, 3143, 3365, 3429, 4090
AfeI	AGCGCT	1 cut(s)	1815
AfiI	CCNNNNNNNGG	9 cut(s)	44, 263, 1607, 1687, 2503, 2701, 2840, 3224, 4363
AflII	CTTAAG	1 cut(s)	1976
AflIII	ACRYGT	2 cut(s)	3360, 3691
AgeI	ACCGGT	1 cut(s)	4032
AhdI	GACNNNNNGTC	1 cut(s)	498
AjiI	CACGTC	1 cut(s)	4055
AjnI	CCWGG	6 cut(s)	338, 565, 1249, 1847, 2496, 3547
AleI	CACNNNNGTG	1 cut(s)	2597
Alw21I	GWGCWC	7 cut(s)	237, 1435, 1735, 2586, 3013, 3529, 4257
Alw26I	GTCTC	7 cut(s)	162, 881, 918, 1069, 1914, 2618, 4273
Alw44I	GTGCAC	1 cut(s)	1731
AlwNI	CAGNNNCTG	4 cut(s)	602, 791, 1703, 2867
Ama87I	CYCGRG	4 cut(s)	230, 832, 4016, 4250
Aor13HI	TCCGGA	1 cut(s)	13
Aor51HI	AGCGCT	1 cut(s)	1815
AoxI	GGCC	5 cut(s)	99, 375, 1474, 2494, 4171
ApaLI	GTGCAC	1 cut(s)	1731
ApoI	RAATTY	8 cut(s)	251, 1356, 1423, 1876, 1886, 2009, 3068, 4375
ArsI	GACNNNNNNTTYG	4 cut(s)	1378, 1410, 2981, 3013
AseI	ATTAAT	1 cut(s)	2037
AsiGI	ACCGGT	1 cut(s)	4032
Asp700I	GAANNNNTTC	5 cut(s)	1522, 1761, 2754, 4080, 4509
AspLEI	GCGC	5 cut(s)	149, 1278, 1816, 2959, 4347
AspS9I	GGNCC	6 cut(s)	405, 464, 773, 2053, 2484, 2494
AsuC2I	CCSGG	1 cut(s)	2057
AsuHPI	GGTGA	2 cut(s)	3951, 4394
AvaI	CYCGRG	4 cut(s)	230, 832, 4016, 4250
AvaII	GGWCC	5 cut(s)	405, 464, 773, 2053, 2484
AxyI	CCTNAGG	1 cut(s)	2817
BaeGI	GKGCMC	2 cut(s)	130, 1735
BalI	TGGCCA	1 cut(s)	4173
BamHI	GGATCC	2 cut(s)	10, 4271
BanI	GGYRCC	3 cut(s)	2191, 3185, 4353
BanII	GRGCYC	3 cut(s)	237, 3013, 3453
BbsI	GAAGAC	4 cut(s)	3052, 3582, 3908, 3963
Bbv12I	GWGCWC	7 cut(s)	237, 1435, 1735, 2586, 3013, 3529, 4257
BceAI	ACGGC	2 cut(s)	538, 1932
BcgI	CGANNNNNNTGC	2 cut(s)	221, 255
BciT130I	CCWGG	6 cut(s)	340, 567, 1251, 1849, 2498, 3549
BciVI	GTATCC	1 cut(s)	3555
BcnI	CCSGG	1 cut(s)	2057
BcoDI	GTCTC	7 cut(s)	162, 881, 918, 1069, 1914, 2618, 4273
BfaI	CTAG	4 cut(s)	414, 1862, 2126, 3561
BfmI	CTRYAG	1 cut(s)	2646
BfoI	RGCGCY	2 cut(s)	1817, 4348
BfrI	CTTAAG	1 cut(s)	1976
BfuI	GTATCC	1 cut(s)	3555
BglII	AGATCT	2 cut(s)	1828, 3079
BmcAI	AGTACT	1 cut(s)	3429
Bme1390I	CCNGG	7 cut(s)	340, 567, 1251, 1849, 2057, 2498, 3549
Bme18I	GGWCC	5 cut(s)	405, 464, 773, 2053, 2484
BmeRI	GACNNNNNGTC	1 cut(s)	498
BmeT110I	CYCGRG	4 cut(s)	230, 832, 4016, 4250
BmgBI	CACGTC	1 cut(s)	4055
BmgT120I	GGNCC	6 cut(s)	405, 464, 773, 2053, 2484, 2494
BmrFI	CCNGG	7 cut(s)	340, 567, 1251, 1849, 2057, 2498, 3549
BpiI	GAAGAC	4 cut(s)	3052, 3582, 3908, 3963
BplI	GAGNNNNNCTC	2 cut(s)	3511, 3543
Bpu10I	CCTNAGC	3 cut(s)	1658, 2555, 2700
BpuEI	CTTGAG	5 cut(s)	1034, 2204, 2981, 3172, 4382
BpuMI	CCSGG	1 cut(s)	2057
Bsa29I	ATCGAT	1 cut(s)	2847
BsaAI	YACGTR	2 cut(s)	898, 1235
BsaBI	GATNNNNATC	4 cut(s)	329, 2106, 3084, 3600
BsaHI	GRCGYC	1 cut(s)	3157
BsaI	GGTCTC	2 cut(s)	162, 4273
BsaJI	CCNNGG	3 cut(s)	43, 372, 1848
BsaWI	WCCGGW	4 cut(s)	13, 494, 4032, 4379
BsaXI	ACNNNNNCTCC	2 cut(s)	3125, 3155
Bsc4I	CCNNNNNNNGG	9 cut(s)	44, 263, 1607, 1687, 2503, 2701, 2840, 3224, 4363
Bse118I	RCCGGY	2 cut(s)	1496, 4032
Bse21I	CCTNAGG	1 cut(s)	2817
Bse3DI	GCAATG	2 cut(s)	1468, 2904
Bse8I	GATNNNNATC	4 cut(s)	329, 2106, 3084, 3600
BseAI	TCCGGA	1 cut(s)	13
BseBI	CCWGG	6 cut(s)	340, 567, 1251, 1849, 2498, 3549
BseCI	ATCGAT	1 cut(s)	2847
BseDI	CCNNGG	3 cut(s)	43, 372, 1848
BseJI	GATNNNNATC	4 cut(s)	329, 2106, 3084, 3600
BseLI	CCNNNNNNNGG	9 cut(s)	44, 263, 1607, 1687, 2503, 2701, 2840, 3224, 4363
BseMI	GCAATG	2 cut(s)	1468, 2904
BseMII	CTCAG	6 cut(s)	897, 1080, 1302, 2714, 2831, 3855
BseRI	GAGGAG	3 cut(s)	989, 2636, 3146
BseSI	GKGCMC	2 cut(s)	130, 1735
BseYI	CCCAGC	1 cut(s)	425
BsgI	GTGCAG	2 cut(s)	671, 2550
BshFI	GGCC	5 cut(s)	101, 377, 1476, 2496, 4173
BshNI	GGYRCC	3 cut(s)	2191, 3185, 4353
BshTI	ACCGGT	1 cut(s)	4032
BshVI	ATCGAT	1 cut(s)	2847
BsiHKAI	GWGCWC	7 cut(s)	237, 1435, 1735, 2586, 3013, 3529, 4257
BsiHKCI	CYCGRG	4 cut(s)	230, 832, 4016, 4250
BsiSI	CCGG	7 cut(s)	14, 378, 495, 1497, 2057, 4033, 4380
BsiWI	CGTACG	1 cut(s)	4088
BslFI	GGGAC	3 cut(s)	418, 2039, 2983
BslI	CCNNNNNNNGG	9 cut(s)	44, 263, 1607, 1687, 2503, 2701, 2840, 3224, 4363
BsmAI	GTCTC	7 cut(s)	162, 881, 918, 1069, 1914, 2618, 4273
BsmFI	GGGAC	3 cut(s)	418, 2039, 2983
BsmI	GAATGC	2 cut(s)	1144, 1254
BsnI	GGCC	5 cut(s)	101, 377, 1476, 2496, 4173
Bso31I	GGTCTC	2 cut(s)	162, 4273
BsoBI	CYCGRG	4 cut(s)	230, 832, 4016, 4250
Bsp1286I	GDGCHC	9 cut(s)	130, 237, 1435, 1735, 2586, 3013, 3453, 3529, 4257
Bsp13I	TCCGGA	1 cut(s)	13
Bsp1407I	TGTACA	1 cut(s)	858
BspACI	CCGC	5 cut(s)	102, 108, 480, 1561, 2775
BspANI	GGCC	5 cut(s)	101, 377, 1476, 2496, 4173
BspCNI	CTCAG	6 cut(s)	896, 1079, 1303, 2713, 2830, 3854
BspDI	ATCGAT	1 cut(s)	2847
BspEI	TCCGGA	1 cut(s)	13
BspHI	TCATGA	2 cut(s)	2746, 4293
BspQI	GCTCTTC	1 cut(s)	3534
BspT107I	GGYRCC	3 cut(s)	2191, 3185, 4353
BspTI	CTTAAG	1 cut(s)	1976
BspTNI	GGTCTC	2 cut(s)	162, 4273
BsrBI	CCGCTC	1 cut(s)	1561
BsrDI	GCAATG	2 cut(s)	1468, 2904
BsrFI	RCCGGY	2 cut(s)	1496, 4032
BsrGI	TGTACA	1 cut(s)	858
BssAI	RCCGGY	2 cut(s)	1496, 4032
BssECI	CCNNGG	3 cut(s)	43, 372, 1848
BssNAI	GTATAC	1 cut(s)	4332
BssNI	GRCGYC	1 cut(s)	3157
Bst1107I	GTATAC	1 cut(s)	4332
Bst2UI	CCWGG	6 cut(s)	340, 567, 1251, 1849, 2498, 3549
Bst4CI	ACNGT	8 cut(s)	373, 640, 781, 1504, 2518, 2636, 2984, 3784
Bst6I	CTCTTC	4 cut(s)	301, 1917, 3534, 4086
BstACI	GRCGYC	1 cut(s)	3157
BstAFI	CTTAAG	1 cut(s)	1976
BstAPI	GCANNNNNTGC	2 cut(s)	791, 3107
BstAUI	TGTACA	1 cut(s)	858
BstBAI	YACGTR	2 cut(s)	898, 1235
BstDSI	CCRYGG	1 cut(s)	372
BstEII	GGTNACC	1 cut(s)	3407
BstENI	CCTNNNNNAGG	1 cut(s)	2699
BstH2I	RGCGCY	2 cut(s)	1817, 4348
BstHHI	GCGC	5 cut(s)	149, 1278, 1816, 2959, 4347
BstMAI	GTCTC	7 cut(s)	162, 881, 918, 1069, 1914, 2618, 4273
BstNI	CCWGG	6 cut(s)	340, 567, 1251, 1849, 2498, 3549
BstNSI	RCATGY	4 cut(s)	1064, 2940, 3364, 3695
BstPI	GGTNACC	1 cut(s)	3407
BstSCI	CCNGG	7 cut(s)	338, 565, 1249, 1847, 2055, 2496, 3547
BstSFI	CTRYAG	1 cut(s)	2646
BstSLI	GKGCMC	2 cut(s)	130, 1735
BstSNI	TACGTA	2 cut(s)	898, 1235
BstV2I	GAAGAC	4 cut(s)	3052, 3582, 3908, 3963
BstX2I	RGATCY	6 cut(s)	10, 730, 1828, 2536, 3079, 4271
BstYI	RGATCY	6 cut(s)	10, 730, 1828, 2536, 3079, 4271
BstZ17I	GTATAC	1 cut(s)	4332
Bsu15I	ATCGAT	1 cut(s)	2847
Bsu36I	CCTNAGG	1 cut(s)	2817
BsuI	GTATCC	1 cut(s)	3555
BsuRI	GGCC	5 cut(s)	101, 377, 1476, 2496, 4173
BsuTUI	ATCGAT	1 cut(s)	2847
BtgI	CCRYGG	1 cut(s)	372
BtgZI	GCGATG	1 cut(s)	142
BtrI	CACGTC	1 cut(s)	4055
BtsI	GCAGTG	3 cut(s)	481, 558, 2538
CaiI	CAGNNNCTG	4 cut(s)	602, 791, 1703, 2867
CciI	TCATGA	2 cut(s)	2746, 4293
CfoI	GCGC	5 cut(s)	149, 1278, 1816, 2959, 4347
Cfr10I	RCCGGY	2 cut(s)	1496, 4032
Cfr13I	GGNCC	6 cut(s)	405, 464, 773, 2053, 2484, 2494
ClaI	ATCGAT	1 cut(s)	2847
CseI	GACGC	1 cut(s)	3146
Csp6I	GTAC	9 cut(s)	216, 584, 859, 2465, 2717, 3142, 3364, 3428, 4089
CspAI	ACCGGT	1 cut(s)	4032
CspCI	CAANNNNNGTGG	4 cut(s)	1755, 1790, 3401, 3436
CviQI	GTAC	9 cut(s)	216, 584, 859, 2465, 2717, 3142, 3364, 3428, 4089
DraIII	CACNNNGTG	2 cut(s)	1472, 2711
DriI	GACNNNNNGTC	1 cut(s)	498
EaeI	YGGCCR	3 cut(s)	99, 375, 4171
Eam1104I	CTCTTC	4 cut(s)	301, 1917, 3534, 4086
Eam1105I	GACNNNNNGTC	1 cut(s)	498
EarI	CTCTTC	4 cut(s)	301, 1917, 3534, 4086
Ecl136II	GAGCTC	2 cut(s)	235, 3011
Eco105I	TACGTA	2 cut(s)	898, 1235
Eco24I	GRGCYC	3 cut(s)	237, 3013, 3453
Eco31I	GGTCTC	2 cut(s)	162, 4273
Eco32I	GATATC	1 cut(s)	331
Eco47I	GGWCC	5 cut(s)	405, 464, 773, 2053, 2484
Eco47III	AGCGCT	1 cut(s)	1815
Eco53kI	GAGCTC	2 cut(s)	235, 3011
Eco57I	CTGAAG	8 cut(s)	285, 2040, 2543, 2752, 3411, 3452, 3648, 3957
Eco81I	CCTNAGG	1 cut(s)	2817
Eco88I	CYCGRG	4 cut(s)	230, 832, 4016, 4250
Eco91I	GGTNACC	1 cut(s)	3407
EcoICRI	GAGCTC	2 cut(s)	235, 3011
EcoNI	CCTNNNNNAGG	1 cut(s)	2699
EcoO65I	GGTNACC	1 cut(s)	3407
EcoRII	CCWGG	6 cut(s)	338, 565, 1249, 1847, 2496, 3547
EcoRV	GATATC	1 cut(s)	331
EcoT22I	ATGCAT	5 cut(s)	367, 1022, 3103, 3319, 3330
EcoT38I	GRGCYC	3 cut(s)	237, 3013, 3453
FalI	AAGNNNNNCTT	6 cut(s)	3033, 3065, 3281, 3313, 4461, 4493
FaqI	GGGAC	3 cut(s)	418, 2039, 2983
FauI	CCCGC	1 cut(s)	1554
FauNDI	CATATG	2 cut(s)	188, 3493
FblI	GTMKAC	2 cut(s)	4057, 4331
FriOI	GRGCYC	3 cut(s)	237, 3013, 3453
FspBI	CTAG	4 cut(s)	414, 1862, 2126, 3561
FspI	TGCGCA	1 cut(s)	1277
GlaI	GCGC	5 cut(s)	148, 1277, 1815, 2958, 4346
GsaI	CCCAGC	1 cut(s)	429
HaeII	RGCGCY	2 cut(s)	1817, 4348
HaeIII	GGCC	5 cut(s)	101, 377, 1476, 2496, 4173
HapII	CCGG	7 cut(s)	14, 378, 495, 1497, 2057, 4033, 4380
HgaI	GACGC	1 cut(s)	3146
HhaI	GCGC	5 cut(s)	149, 1278, 1816, 2959, 4347
Hin1I	GRCGYC	1 cut(s)	3157
Hin6I	GCGC	5 cut(s)	147, 1276, 1814, 2957, 4345
HinP1I	GCGC	5 cut(s)	147, 1276, 1814, 2957, 4345
HincII	GTYRAC	1 cut(s)	3924
HindII	GTYRAC	1 cut(s)	3924
HindIII	AAGCTT	4 cut(s)	1603, 2342, 2809, 2976
HpaII	CCGG	7 cut(s)	14, 378, 495, 1497, 2057, 4033, 4380
HphI	GGTGA	2 cut(s)	3951, 4394
Hpy166II	GTNNAC	6 cut(s)	1733, 2361, 3924, 3959, 4058, 4332
Hpy8I	GTNNAC	6 cut(s)	1733, 2361, 3924, 3959, 4058, 4332
Hpy99I	CGWCG	1 cut(s)	1402
HpyAV	CCTTC	6 cut(s)	1169, 1345, 2924, 3232, 3256, 3427
HpyCH4III	ACNGT	8 cut(s)	373, 640, 781, 1504, 2518, 2636, 2984, 3784
HpyCH4IV	ACGT	5 cut(s)	897, 1234, 2063, 3181, 4054
HpySE526I	ACGT	5 cut(s)	897, 1234, 2063, 3181, 4054
Hsp92I	GRCGYC	1 cut(s)	3157
HspAI	GCGC	5 cut(s)	147, 1276, 1814, 2957, 4345
Kpn2I	TCCGGA	1 cut(s)	13
LguI	GCTCTTC	1 cut(s)	3534
LmnI	GCTCC	7 cut(s)	342, 568, 788, 912, 1944, 2883, 3016
MaeI	CTAG	4 cut(s)	414, 1862, 2126, 3561
MaeII	ACGT	5 cut(s)	897, 1234, 2063, 3181, 4054
MaeIII	GTNAC	5 cut(s)	1666, 2298, 3407, 3849, 4382
MbiI	CCGCTC	1 cut(s)	1561
MfeI	CAATTG	4 cut(s)	1685, 2268, 3416, 3570
MflI	RGATCY	6 cut(s)	10, 730, 1828, 2536, 3079, 4271
MhlI	GDGCHC	9 cut(s)	130, 237, 1435, 1735, 2586, 3013, 3453, 3529, 4257
MlsI	TGGCCA	1 cut(s)	4173
MluNI	TGGCCA	1 cut(s)	4173
MlyI	GAGTC	6 cut(s)	390, 824, 1322, 2099, 3005, 4457
MmeI	TCCRAC	9 cut(s)	244, 494, 800, 1032, 1269, 1593, 3593, 4129, 4471
Mox20I	TGGCCA	1 cut(s)	4173
Mph1103I	ATGCAT	5 cut(s)	367, 1022, 3103, 3319, 3330
MroI	TCCGGA	1 cut(s)	13
MroXI	GAANNNNTTC	5 cut(s)	1522, 1761, 2754, 4080, 4509
MscI	TGGCCA	1 cut(s)	4173
MslI	CAYNNNNRTG	5 cut(s)	32, 915, 1263, 2597, 3856
Msp20I	TGGCCA	1 cut(s)	4173
MspA1I	CMGCKG	1 cut(s)	2264
MspCI	CTTAAG	1 cut(s)	1976
MspI	CCGG	7 cut(s)	14, 378, 495, 1497, 2057, 4033, 4380
MspR9I	CCNGG	7 cut(s)	340, 567, 1251, 1849, 2057, 2498, 3549
MunI	CAATTG	4 cut(s)	1685, 2268, 3416, 3570
Mva1269I	GAATGC	2 cut(s)	1144, 1254
MvaI	CCWGG	6 cut(s)	340, 567, 1251, 1849, 2498, 3549
NciI	CCSGG	1 cut(s)	2057
NdeI	CATATG	2 cut(s)	188, 3493
NmeAIII	GCCGAG	1 cut(s)	24
NmuCI	GTSAC	3 cut(s)	1666, 3849, 4382
NsbI	TGCGCA	1 cut(s)	1277
NsiI	ATGCAT	5 cut(s)	367, 1022, 3103, 3319, 3330
NspI	RCATGY	4 cut(s)	1064, 2940, 3364, 3695
OliI	CACNNNNGTG	1 cut(s)	2597
PaeR7I	CTCGAG	4 cut(s)	230, 832, 4016, 4250
PagI	TCATGA	2 cut(s)	2746, 4293
PciI	ACATGT	2 cut(s)	3360, 3691
PciSI	GCTCTTC	1 cut(s)	3534
PcsI	WCGNNNNNNNCGW	2 cut(s)	228, 2853
PctI	GAATGC	2 cut(s)	1144, 1254
PdmI	GAANNNNTTC	5 cut(s)	1522, 1761, 2754, 4080, 4509
Pfl23II	CGTACG	1 cut(s)	4088
PflFI	GACNNNGTC	1 cut(s)	1312
PflMI	CCANNNNNTGG	4 cut(s)	1607, 1687, 2840, 3224
PfoI	TCCNGGA	1 cut(s)	338
PinAI	ACCGGT	1 cut(s)	4032
PleI	GAGTC	6 cut(s)	389, 824, 1321, 2098, 3004, 4457
PpsI	GAGTC	6 cut(s)	389, 824, 1321, 2098, 3004, 4457
Ppu21I	YACGTR	2 cut(s)	898, 1235
PscI	ACATGT	2 cut(s)	3360, 3691
PshBI	ATTAAT	1 cut(s)	2037
PsiI	TTATAA	3 cut(s)	1296, 2919, 3164
Psp124BI	GAGCTC	2 cut(s)	237, 3013
Psp6I	CCWGG	6 cut(s)	338, 565, 1249, 1847, 2496, 3547
PspEI	GGTNACC	1 cut(s)	3407
PspFI	CCCAGC	1 cut(s)	425
PspGI	CCWGG	6 cut(s)	338, 565, 1249, 1847, 2496, 3547
PspLI	CGTACG	1 cut(s)	4088
PspPI	GGNCC	6 cut(s)	405, 464, 773, 2053, 2484, 2494
PspXI	VCTCGAGB	3 cut(s)	230, 832, 4250
PsrI	GAACNNNNNNTAC	2 cut(s)	4028, 4060
PstNI	CAGNNNCTG	4 cut(s)	602, 791, 1703, 2867
PsuI	RGATCY	6 cut(s)	10, 730, 1828, 2536, 3079, 4271
PsyI	GACNNNGTC	1 cut(s)	1312
PvuII	CAGCTG	1 cut(s)	2264
RsaI	GTAC	9 cut(s)	217, 585, 860, 2466, 2718, 3143, 3365, 3429, 4090
RsaNI	GTAC	9 cut(s)	216, 584, 859, 2465, 2717, 3142, 3364, 3428, 4089
RseI	CAYNNNNRTG	5 cut(s)	32, 915, 1263, 2597, 3856
SacI	GAGCTC	2 cut(s)	237, 3013
SapI	GCTCTTC	1 cut(s)	3534
Sau96I	GGNCC	6 cut(s)	405, 464, 773, 2053, 2484, 2494
ScaI	AGTACT	1 cut(s)	3429
SchI	GAGTC	6 cut(s)	390, 824, 1322, 2099, 3005, 4457
ScrFI	CCNGG	7 cut(s)	340, 567, 1251, 1849, 2057, 2498, 3549
SduI	GDGCHC	9 cut(s)	130, 237, 1435, 1735, 2586, 3013, 3453, 3529, 4257
SfcI	CTRYAG	1 cut(s)	2646
Sfr274I	CTCGAG	4 cut(s)	230, 832, 4016, 4250
SinI	GGWCC	5 cut(s)	405, 464, 773, 2053, 2484
SlaI	CTCGAG	4 cut(s)	230, 832, 4016, 4250
SmiMI	CAYNNNNRTG	5 cut(s)	32, 915, 1263, 2597, 3856
SnaBI	TACGTA	2 cut(s)	898, 1235
SsiI	CCGC	5 cut(s)	102, 108, 480, 1561, 2775
SspI	AATATT	4 cut(s)	1182, 1407, 1597, 2923
SspMI	CTAG	4 cut(s)	414, 1862, 2126, 3561
SstI	GAGCTC	2 cut(s)	237, 3013
StyD4I	CCNGG	7 cut(s)	338, 565, 1249, 1847, 2055, 2496, 3547
TaaI	ACNGT	8 cut(s)	373, 640, 781, 1504, 2518, 2636, 2984, 3784
TaiI	ACGT	5 cut(s)	900, 1237, 2066, 3184, 4057
TatI	WGTACW	4 cut(s)	858, 2716, 3141, 3427
TauI	GCSGC	3 cut(s)	104, 482, 2777
TseFI	GTSAC	3 cut(s)	1666, 3849, 4382
Tsp45I	GTSAC	3 cut(s)	1666, 3849, 4382
TspGWI	ACGGA	5 cut(s)	477, 722, 1050, 4106, 4317
Tth111I	GACNNNGTC	1 cut(s)	1312
Van91I	CCANNNNNTGG	4 cut(s)	1607, 1687, 2840, 3224
Vha464I	CTTAAG	1 cut(s)	1976
VneI	GTGCAC	1 cut(s)	1731
VpaK11BI	GGWCC	5 cut(s)	405, 464, 773, 2053, 2484
VspI	ATTAAT	1 cut(s)	2037
XagI	CCTNNNNNAGG	1 cut(s)	2699
XapI	RAATTY	8 cut(s)	251, 1356, 1423, 1876, 1886, 2009, 3068, 4375
XceI	RCATGY	4 cut(s)	1064, 2940, 3364, 3695
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3495, 4317
XhoI	CTCGAG	4 cut(s)	230, 832, 4016, 4250
XmiI	GTMKAC	2 cut(s)	4057, 4331
XmnI	GAANNNNTTC	5 cut(s)	1522, 1761, 2754, 4080, 4509
XspI	CTAG	4 cut(s)	414, 1862, 2126, 3561
ZrmI	AGTACT	1 cut(s)	3429
Zsp2I	ATGCAT	5 cut(s)	367, 1022, 3103, 3319, 3330

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.