Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0002601

Physical Location & Seq

Genomic Coordinates Reverse (-)

43439885 .. 43455048

Gene Structure 15164 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG330700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4533 bp

ATGGAAGTGCCAATCCTCGGCAGCGATTCACTGAAATGGATTGAGTTCCCTATTCCTTCTTCTAATGCCGCCACCGCCACCGCCACCGCCACCGCCACCGCCACCGCCACCGCCACCGCCACCGCCACCGCCACCGCCGTTGCTGATACTTGTGCCCCTCTCACTCACGATTGCGCTTCTTCCATCGCCATTGGCGACCCTCCTACACATCTCATATGGAGAATCCACAAGCATCTCCCTCACTCTCTCGAATTACTTGAGCTCTCTGCTAACAAAGAATTTCCAAAACTCGGACTGCGAATCACTTTTCCACAAGCACTCTCTTCCTCCGTTTTTCTTTGCAAAAATGAGATTGATGTCGGTTCCAGGAGCCACCCCTATTTGCTCTATGCATTGACCGTTGCCGGAGTCGCTTACCTTTTGAGACTTGGAACCGTCTCCAGTTATGCTTCCAACTCTGTTATTAGAGAGTTTAGCTTGCATCCCTACGGACCTATAACCAGTGCCGCAGCAACGCCAACCGGATGTCTTGTTGTAGGTCGGAATGATGGCTCACTTGCTTGCTTTCAACTCACACTTGAATCCACTGCTCCTGGTTTTCTGCAAGAGCTACGGGATGATCCAGGAATCGGTAGTTTGTGGGGTTTCATGTCAAGGGGTAGGATGGTAGGCGCTGTGCAGGACTTGGCTATATCTATGGTTCATGGGAAACCACTTATATTCGTTATTCATACGGACGGGGTGTTACGAGTATGGGATATATCATTTCATAGCAGGCTCTTTTCCCATAAGATGAACAGTCTAACAATGGCAGGAGCTGCATTGGTAAGGTTGTGGGTTGGTCAAGCTGACAATGATTCGAGCATAATTCCTTTGGCAATCTTGTACAGACATGATTCGGAAATGAGCTCAGAGACGATCTACGTATATAGCCTCCATTGTAACGTGGGAGACAGAATTGTTTTATCGATGGATCCTTCAGAACAAATTATTCCTATTGAGGAGGATGGATGGATTGATGTCAAACTTATTTCCAACAAGATATGCATATTGAAAAATCAGGGACTGGTGCTTCACAAGTTGTTGCATACAAATGTCAACATGGTAGATGCAGTTTGTTATGCTTTGCAAGAGGACTTCGTTGCAGATCAACTCTTTCAAAGTTCTGAACATTCTTCGGATGACCTTCTTTTGATAACTCATTCAATATTTTCATCTTCAAAGGATCATATTCTACCCGTTGTATCTTCTATTTTCTTACGTAGGCTGCTTCTTCCTGGCATTCATCATAATGCTGCTTTGCGCACAACTTTGTTGGATTATAATAGGCACTGGACTGAGTCAGATTTCCACTCCTTAACTGCTGACGGTTTGAAGAAGGAAATTCTTTCCCTGATTGAACATGAGGGTCTGACTGGTAATTCATCGTCAATATTTTGCTGCTGGAAAATTTTTTGTGCTCGCTATTTCCAGAACTGGTGCAAGAGCAATGCACCATGTGGCCTACTTGTTGATTCCTCAACCGGCACTGTTGGTTTAATTAGAAAGAGTTCAGTATCTCTTTTTCGTTCTTTGGAAGATATTGAGCGGGTTAATGATGGCTCTTTGGATGAACTTGGCAATTTTCCAAGCTTTGGGTTGGATTCATTTGATGAGGCTCGTGATTGTGAGCTACTTGCTGAAATGCTCAGATGTGTCATTAACGTCAGCCAGCAATTGGGCAAAACAGCTTCTGCTATATTTTATGAATCACTCATTAGTGCACCACCAGTTATCTCATCTGAAGACATTATTCCGCGCCTATTGAAGATATTAGAAACTGGATATAGTTCAACAGCCGCAGTGCTTCATATATCAGATCTTGGACCTGATGTTGCCTGGGAGAAAAATCTAGCAGATCACAAAAATTTGAGGAAATTTTCAATTGACATGATGTTGTCTCTTCATGCTTTGCACGAGAAATCTGGCACATGGAGCCGTATTTTGAGTGTGATTGAGAACTATCTAAAGTATCTCATACCTCGGAAAATTATACAAAAATTTGATGCTGTAGTAGCATTGGATATCAATGCCTCCATCTTGGTCCAGGCCATGTCTCAAGTTGCAAAGGTTATGTTTGAGTCTGCACTGGATATTCATCTGTTTCTAAGCTATCTAGTAAGCATTAGTGGGCAGATCAATATGCTACATGATGATATATCCAAAATACAGCTTGAGTTGGTTCCGATGATTCAAGAAATCATTTATGAGTGGCTTCTCTTACATTTCTTTGCCACCACACCATCTGAATCAGCTGCAATTGAAGATTTCAGCTCTCAACTTTCATTGTTACAAATTGATAGCAACACAGGCAGAAGATCATGGAATGAGAAGCTTGGGAAGTGCGAGTTCACTCTGGCTTTTATATTCTTACTTAATGTTAGAAGTTCCTCCAAAGACCAGAGCCACTTTTGTTCAAGAAGCATATCAAATGTTCAGGACATAATTGATTCAATACGAGAATTTGCTAGCTGGATCATGTGGGGCCAGAATAGGGAATCCCTTACTGTCCTGAGGCGTGCAGCGGATCTTGCATTGATCCTGCTAAGGCACGGGCAGTATGATGCTGTTGAGCACCTACTCACTATTGTGGAGGCACATTTACAAAAGGAGAAGACTTCTCATCGTGTTCAAGCTACAGATGGCGGATGGTGCATACTGCATCATCTTTTAGGATGCTGTTATCTTGCTCAAGCACATCGTGGATTACATGGGGTCCTGAAAGAGAGGAAAGTTCATGAAGCTGTATGTTGTTTCTTCAGAGCTGCATCTGGAAATGGCTCAGCTCAGGCTTTACAAAGCTTGCCTCAGGAAGCGGGGTTGCCACATCTTGGCATCGATGGTAGCATATCAGATGCTGCATGGAAGCTCCATTACTATCAGTGGGCAATGCAGATATTTGAGCAATATAATATTAGTGAAGGTGCATGTCAGTTTGCTCTTGCTGCGCTTGAGCAAGTTGAGGAAGCGTATAGTGCAAACACTGAGTCCCATGACAGAGCTCCATTTGATGAATCAGTATCTACCATTAAAGGAAGACTTTGGGCACATGTTTGCAAGTTTACTTTAGATCTCAATCTCTTTTATGATGCGTACTGTGCAATAATTTCAAATCCAGATGAGGAGAGCAAGCACATCTGGTTGAGGCGTCTTATAATTGTTCTTTATGAACGTGGTGCCATAAAGATTCTTTGTGGTGGTCAGCTACCATTCATTGGTCTTACAGAGAAGGTAGAACAAGAGCTTCCATGGAAGGCAGAGCGGTCAGACATTTTAGCGAGGCCAAACTTATATAAGTTGCTTTATGCATTTGAAATGCATCGGCATAACTGGCGAAAAGCAGCAAGCTACATGTACCTTTATTCTGCTCGTTTGAGAACTGAAACATCTCTGAAAGGGTACCAGCAATTGTGGCGAGCACTGAAGGAGATACTGAATGGGCTCTCTGCTGCTATCAACGCACTTCATCTTGTTCATCCAGCATATGCTTGGATTGATCCTCTCCTTGAGAGAAATGCTATTCACAATGAGCAGTATCCAAGTAAAAAGGCTAGAATAACAATTGAAGACCAGTCAGCAAGCAATGATGTTGATCCTCAAAGTTGGAAATCTTATATAGATATTCAGAAAATTGAAAACGAGTTTGTGCTTACTTCAGCAGAATATTTGCTTTCACTAGCACATGTCAAATGGACAAATACAGGAACCCAGAAAGCACCATTGGAGTTGGTTGATCTCTTGATCCAGACAAATCTATATGATATGGCATTTACAGTTCTCTTAAGATTCTTCAAAGGTTCAGAGTTAAAGAGGGGATTGGAAAGAGTTTTCTCTGCTATGTCACTAAAATGCTGTCCGCATATAGTTAATTCGTCAAGGGTCGGGGATGATTCCACAACACAGGGTCTTCTGTTGACTTCTTCAAAGGATGAAGTGATCGTTCATGGTTCACCAGATACAAGTTCAGCAAATCAACAATCCAGTGGAATTAATCAATGGGGAACTCTCGAGCTCTATCTTGACCGATATAAAGTCTTCCATGCACGTCTACCATTTATTGTTGCTGAAACTCTTCTTCGTACGGATTCTCAAATCGAACTGCCTCTTTGGTTGGTTAAATTATTCAAGGATGGCAGGAGGGAAAAGACATTGGGGATGACTGGCCAAGAATCAAGTCCGGCATTATTATTTCAACTATATGTTGATTATGGTCGATATAGAGAAGCTACGAATCTGTTGCTTGAGTACATAGGTTCTTTAGCATCCATGAGACCAGCCAACATCATTAATCGTAAAAGACCATTTGGAGTTTGGTTTCCATATACAACAATTCAGCGCCTCTGGTGCCGACTCGAGGAAATGATAAATTCAGGTCACATGGTAGATCAATGTAAGCAGCTCAAGGATTTGCTACATGGAGCTTTGCTGAAACATCTCCAACTGGTAAAAGTGGACTCGGAAGATGCACTCGAGTCATTTCAGAAAATAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0008104 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016973 GO:0031090 GO:0031503 GO:0031965 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0034613 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

DUF5311 Nup160

Protein Analysis

1510

Amino Acids

169.33

Weight (kDa)

6.1

Isoelectric Point (pI)

42.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_Nup120_160	PF11715	71 - 529	2.1e-55	Nucleoporin Nup120/160, beta-propeller domain
NUP160_helical_2	PF17238	546 - 740	6.1e-91	NUP160, helical domain
TPR_NUP160_120_M	PF23354	919 - 1170	4.6e-34	NUP160/120 middle TPR
HEAT_Nup120	PF23300	920 - 1069	4.4e-12	Nucleoporin Nup120, HEAT repeats
TPR_Nup160_C	PF23347	1222 - 1502	6e-13	Nup160 C-terminal TPR

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002601)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33410 AT1G33410
fragaria_vesca	FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400
malus_domestica	MD00G1034800.v1.1 MD05G1063300.v1.1 MD05G1161100.v1.1 MD05G1162300.v1.1 MD05G1162400.v1.1 MD07G1280900.v1.1 MD08G1158600.v1.1 MD10G1009800.v1.1 MD10G1160000.v1.1
prunus_persica	Prupe.5G022000_v2.0.a1
pyrus_communis	pycom10g00600
rosa_chinensis	RchiOBHm_Chr2g0126921 RchiOBHm_Chr4g0402211
rosa_laevigata	RLG00000009106 RLG00000018928
rosa_multiflora	Rmu_sc0000007.1_g000030 Rmu_sc0001405.1_g000051
rosa_roxburghii	Rroxscaffold_1G00047890 Rroxscaffold_2G00116920 Rroxscaffold_5G00346940
rosa_rugosa	Rorug02G0270100 Rorug02G0270200
rosa_samantha	Rh2AG322500 Rh2BG330700 Rh2CG310600 Rh2DG350000 Rh4BG104100 Rh4CG117900 Rh4DG101500
rosa_wichuraiana	Rw2G026160 Rw4G008910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1323, 3194
Acc16I	TGCGCA	1 cut(s)	1304
Acc65I	GGTACC	1 cut(s)	3438
AccB1I	GGYRCC	3 cut(s)	3215, 3438, 4383
AccB7I	CCANNNNNTGG	4 cut(s)	1634, 1717, 2870, 3254
AccBSI	CCGCTC	2 cut(s)	1588, 3301
AccI	GTMKAC	1 cut(s)	4087
AccII	CGCG	1 cut(s)	1798
AcoI	YGGCCR	1 cut(s)	4201
AcsI	RAATTY	8 cut(s)	278, 1383, 1449, 1906, 1916, 2039, 2501, 4405
AcuI	CTGAAG	5 cut(s)	963, 1803, 2782, 3482, 3678
AcyI	GRCGYC	1 cut(s)	3187
AdeI	CACNNNGTG	2 cut(s)	1499, 2741
AfaI	GTAC	6 cut(s)	887, 3134, 3395, 3440, 4120, 4286
AfiI	CCNNNNNNNGG	9 cut(s)	17, 290, 629, 1634, 1717, 2533, 2870, 3254, 4393
AflII	CTTAAG	1 cut(s)	3820
AflIII	ACRYGT	3 cut(s)	3088, 3390, 3721
AjiI	CACGTC	1 cut(s)	4085
AjnI	CCWGG	6 cut(s)	365, 592, 622, 1276, 1877, 2085
AjuI	GAANNNNNNNTTGG	2 cut(s)	3743, 3775
AleI	CACNNNNGTG	1 cut(s)	2627
Alw21I	GWGCWC	8 cut(s)	264, 911, 1462, 1765, 2616, 3043, 3460, 4053
Alw26I	GTCTC	7 cut(s)	418, 442, 908, 945, 1944, 2100, 4303
Alw44I	GTGCAC	1 cut(s)	1761
AlwNI	CAGNNNCTG	4 cut(s)	818, 1066, 1733, 2897
Ama87I	CYCGRG	3 cut(s)	4046, 4391, 4508
AoxI	GGCC	5 cut(s)	1501, 2088, 2524, 3320, 4201
ApaLI	GTGCAC	1 cut(s)	1761
ApoI	RAATTY	8 cut(s)	278, 1383, 1449, 1906, 1916, 2039, 2501, 4405
ArsI	GACNNNNNNTTYG	2 cut(s)	3011, 3043
AseI	ATTAAT	2 cut(s)	4029, 4326
Asp700I	GAANNNNTTC	2 cut(s)	1549, 4110
Asp718I	GGTACC	1 cut(s)	3438
AspLEI	GCGC	6 cut(s)	176, 674, 1305, 1800, 2989, 4377
AspS9I	GGNCC	5 cut(s)	491, 1865, 2083, 2524, 2755
AsuHPI	GGTGA	1 cut(s)	3981
AsuNHI	GCTAGC	1 cut(s)	2507
AvaI	CYCGRG	3 cut(s)	4046, 4391, 4508
AvaII	GGWCC	4 cut(s)	491, 1865, 2083, 2755
AxyI	CCTNAGG	2 cut(s)	2552, 2847
BaeGI	GKGCMC	3 cut(s)	157, 1765, 3088
BalI	TGGCCA	1 cut(s)	4203
BamHI	GGATCC	1 cut(s)	973
BanI	GGYRCC	3 cut(s)	3215, 3438, 4383
BanII	GRGCYC	5 cut(s)	264, 911, 3043, 3483, 4053
BarI	GAAGNNNNNNTAC	2 cut(s)	2897, 2929
BauI	CACGAG	2 cut(s)	1659, 1955
BbsI	GAAGAC	6 cut(s)	1791, 2660, 3082, 3612, 3938, 4066
Bbv12I	GWGCWC	8 cut(s)	264, 911, 1462, 1765, 2616, 3043, 3460, 4053
BceAI	ACGGC	2 cut(s)	122, 1962
BcgI	CGANNNNNNTGC	2 cut(s)	4258, 4292
BciT130I	CCWGG	6 cut(s)	367, 594, 624, 1278, 1879, 2087
BciVI	GTATCC	1 cut(s)	3585
BcoDI	GTCTC	7 cut(s)	418, 442, 908, 945, 1944, 2100, 4303
BfaI	CTAG	5 cut(s)	1892, 2156, 2508, 3591, 3716
BfmI	CTRYAG	2 cut(s)	2049, 2676
BfoI	RGCGCY	2 cut(s)	675, 4378
BfrI	CTTAAG	1 cut(s)	3820
BfuI	GTATCC	1 cut(s)	3585
BglII	AGATCT	2 cut(s)	1858, 3109
BlpI	GCTNAGC	1 cut(s)	2821
Bme1390I	CCNGG	6 cut(s)	367, 594, 624, 1278, 1879, 2087
Bme18I	GGWCC	4 cut(s)	491, 1865, 2083, 2755
BmeT110I	CYCGRG	3 cut(s)	4046, 4391, 4508
BmgBI	CACGTC	1 cut(s)	4085
BmgT120I	GGNCC	5 cut(s)	491, 1865, 2083, 2524, 2755
BmrFI	CCNGG	6 cut(s)	367, 594, 624, 1278, 1879, 2087
BmtI	GCTAGC	1 cut(s)	2511
BpiI	GAAGAC	6 cut(s)	1791, 2660, 3082, 3612, 3938, 4066
BpmI	CTGGAG	1 cut(s)	424
Bpu10I	CCTNAGC	2 cut(s)	2585, 2826
Bpu1102I	GCTNAGC	1 cut(s)	2821
BpuEI	CTTGAG	8 cut(s)	278, 2082, 2234, 2715, 3011, 3566, 4301, 4424
Bsa29I	ATCGAT	2 cut(s)	968, 2877
BsaAI	YACGTR	2 cut(s)	925, 1262
BsaBI	GATNNNNATC	3 cut(s)	2136, 3114, 3630
BsaHI	GRCGYC	1 cut(s)	3187
BsaI	GGTCTC	1 cut(s)	4303
BsaJI	CCNNGG	4 cut(s)	16, 1878, 2021, 3287
BsaWI	WCCGGW	1 cut(s)	521
Bsc4I	CCNNNNNNNGG	9 cut(s)	17, 290, 629, 1634, 1717, 2533, 2870, 3254, 4393
Bse118I	RCCGGY	1 cut(s)	1523
Bse21I	CCTNAGG	2 cut(s)	2552, 2847
Bse3DI	GCAATG	3 cut(s)	1495, 2934, 3628
Bse8I	GATNNNNATC	3 cut(s)	2136, 3114, 3630
BseBI	CCWGG	6 cut(s)	367, 594, 624, 1278, 1879, 2087
BseCI	ATCGAT	2 cut(s)	968, 2877
BseDI	CCNNGG	4 cut(s)	16, 1878, 2021, 3287
BseJI	GATNNNNATC	3 cut(s)	2136, 3114, 3630
BseLI	CCNNNNNNNGG	9 cut(s)	17, 290, 629, 1634, 1717, 2533, 2870, 3254, 4393
BseMI	GCAATG	3 cut(s)	1495, 2934, 3628
BseMII	CTCAG	8 cut(s)	924, 1329, 1702, 2543, 2835, 2840, 2861, 3015
BseRI	GAGGAG	2 cut(s)	1016, 3176
BseSI	GKGCMC	3 cut(s)	157, 1765, 3088
BsgI	GTGCAG	3 cut(s)	698, 2109, 2580
Bsh1236I	CGCG	1 cut(s)	1798
BshFI	GGCC	5 cut(s)	1503, 2090, 2526, 3322, 4203
BshNI	GGYRCC	3 cut(s)	3215, 3438, 4383
BshVI	ATCGAT	2 cut(s)	968, 2877
BsiHKAI	GWGCWC	8 cut(s)	264, 911, 1462, 1765, 2616, 3043, 3460, 4053
BsiHKCI	CYCGRG	3 cut(s)	4046, 4391, 4508
BsiSI	CCGG	4 cut(s)	405, 522, 1524, 4217
BsiWI	CGTACG	1 cut(s)	4118
BslFI	GGGAC	2 cut(s)	1077, 3013
BslI	CCNNNNNNNGG	9 cut(s)	17, 290, 629, 1634, 1717, 2533, 2870, 3254, 4393
BsmAI	GTCTC	7 cut(s)	418, 442, 908, 945, 1944, 2100, 4303
BsmBI	CGTCTC	2 cut(s)	442, 908
BsmFI	GGGAC	2 cut(s)	1077, 3013
BsmI	GAATGC	1 cut(s)	1281
BsnI	GGCC	5 cut(s)	1503, 2090, 2526, 3322, 4203
Bso31I	GGTCTC	1 cut(s)	4303
BsoBI	CYCGRG	3 cut(s)	4046, 4391, 4508
Bsp1407I	TGTACA	1 cut(s)	885
Bsp1720I	GCTNAGC	1 cut(s)	2821
Bsp19I	CCATGG	1 cut(s)	3287
BspANI	GGCC	5 cut(s)	1503, 2090, 2526, 3322, 4203
BspCNI	CTCAG	8 cut(s)	923, 1330, 1701, 2544, 2834, 2839, 2860, 3016
BspDI	ATCGAT	2 cut(s)	968, 2877
BspFNI	CGCG	1 cut(s)	1798
BspHI	TCATGA	1 cut(s)	2776
BspOI	GCTAGC	1 cut(s)	2511
BspT107I	GGYRCC	3 cut(s)	3215, 3438, 4383
BspTI	CTTAAG	1 cut(s)	3820
BspTNI	GGTCTC	1 cut(s)	4303
BsrBI	CCGCTC	2 cut(s)	1588, 3301
BsrDI	GCAATG	3 cut(s)	1495, 2934, 3628
BsrFI	RCCGGY	1 cut(s)	1523
BsrGI	TGTACA	1 cut(s)	885
BssAI	RCCGGY	1 cut(s)	1523
BssECI	CCNNGG	4 cut(s)	16, 1878, 2021, 3287
BssNI	GRCGYC	1 cut(s)	3187
BssSI	CACGAG	2 cut(s)	1659, 1955
BssT1I	CCWWGG	1 cut(s)	3287
Bst2BI	CACGAG	2 cut(s)	1659, 1955
Bst2UI	CCWGG	6 cut(s)	367, 594, 624, 1278, 1879, 2087
Bst4CI	ACNGT	8 cut(s)	400, 436, 800, 1370, 1531, 2548, 3137, 3814
Bst6I	CTCTTC	3 cut(s)	328, 1947, 4116
BstACI	GRCGYC	1 cut(s)	3187
BstAFI	CTTAAG	1 cut(s)	3820
BstAPI	GCANNNNNTGC	1 cut(s)	818
BstAUI	TGTACA	1 cut(s)	885
BstBAI	YACGTR	2 cut(s)	925, 1262
BstDSI	CCRYGG	1 cut(s)	3287
BstFNI	CGCG	1 cut(s)	1798
BstH2I	RGCGCY	2 cut(s)	675, 4378
BstHHI	GCGC	6 cut(s)	176, 674, 1305, 1800, 2989, 4377
BstMAI	GTCTC	7 cut(s)	418, 442, 908, 945, 1944, 2100, 4303
BstNI	CCWGG	6 cut(s)	367, 594, 624, 1278, 1879, 2087
BstNSI	RCATGY	4 cut(s)	2970, 3092, 3394, 3725
BstSCI	CCNGG	6 cut(s)	365, 592, 622, 1276, 1877, 2085
BstSFI	CTRYAG	2 cut(s)	2049, 2676
BstSLI	GKGCMC	3 cut(s)	157, 1765, 3088
BstSNI	TACGTA	2 cut(s)	925, 1262
BstUI	CGCG	1 cut(s)	1798
BstV2I	GAAGAC	6 cut(s)	1791, 2660, 3082, 3612, 3938, 4066
BstX2I	RGATCY	4 cut(s)	973, 1858, 2566, 3109
BstYI	RGATCY	4 cut(s)	973, 1858, 2566, 3109
Bsu15I	ATCGAT	2 cut(s)	968, 2877
Bsu36I	CCTNAGG	2 cut(s)	2552, 2847
BsuI	GTATCC	1 cut(s)	3585
BsuRI	GGCC	5 cut(s)	1503, 2090, 2526, 3322, 4203
BsuTUI	ATCGAT	2 cut(s)	968, 2877
BtgI	CCRYGG	1 cut(s)	3287
BtgZI	GCGATG	1 cut(s)	169
BtrI	CACGTC	1 cut(s)	4085
BtsI	GCAGTG	2 cut(s)	585, 1848
CaiI	CAGNNNCTG	4 cut(s)	818, 1066, 1733, 2897
CciI	TCATGA	1 cut(s)	2776
CfoI	GCGC	6 cut(s)	176, 674, 1305, 1800, 2989, 4377
Cfr10I	RCCGGY	1 cut(s)	1523
Cfr13I	GGNCC	5 cut(s)	491, 1865, 2083, 2524, 2755
ClaI	ATCGAT	2 cut(s)	968, 2877
CseI	GACGC	1 cut(s)	3176
Csp6I	GTAC	6 cut(s)	886, 3133, 3394, 3439, 4119, 4285
CspCI	CAANNNNNGTGG	2 cut(s)	121, 156
CviQI	GTAC	6 cut(s)	886, 3133, 3394, 3439, 4119, 4285
DraIII	CACNNNGTG	2 cut(s)	1499, 2741
EaeI	YGGCCR	1 cut(s)	4201
Eam1104I	CTCTTC	3 cut(s)	328, 1947, 4116
EarI	CTCTTC	3 cut(s)	328, 1947, 4116
EciI	GGCGGA	1 cut(s)	2700
Ecl136II	GAGCTC	4 cut(s)	262, 909, 3041, 4051
Eco105I	TACGTA	2 cut(s)	925, 1262
Eco130I	CCWWGG	1 cut(s)	3287
Eco24I	GRGCYC	5 cut(s)	264, 911, 3043, 3483, 4053
Eco31I	GGTCTC	1 cut(s)	4303
Eco32I	GATATC	1 cut(s)	2065
Eco47I	GGWCC	4 cut(s)	491, 1865, 2083, 2755
Eco53kI	GAGCTC	4 cut(s)	262, 909, 3041, 4051
Eco57I	CTGAAG	5 cut(s)	963, 1803, 2782, 3482, 3678
Eco81I	CCTNAGG	2 cut(s)	2552, 2847
Eco88I	CYCGRG	3 cut(s)	4046, 4391, 4508
EcoICRI	GAGCTC	4 cut(s)	262, 909, 3041, 4051
EcoO109I	RGGNCCY	1 cut(s)	2755
EcoRII	CCWGG	6 cut(s)	365, 592, 622, 1276, 1877, 2085
EcoRV	GATATC	1 cut(s)	2065
EcoT14I	CCWWGG	1 cut(s)	3287
EcoT22I	ATGCAT	4 cut(s)	394, 1049, 3349, 3360
EcoT38I	GRGCYC	5 cut(s)	264, 911, 3043, 3483, 4053
ErhI	CCWWGG	1 cut(s)	3287
Esp3I	CGTCTC	2 cut(s)	442, 908
FalI	AAGNNNNNCTT	2 cut(s)	3063, 3095
FaqI	GGGAC	2 cut(s)	1077, 3013
FauI	CCCGC	2 cut(s)	1581, 2848
FauNDI	CATATG	2 cut(s)	215, 3523
FblI	GTMKAC	1 cut(s)	4087
FriOI	GRGCYC	5 cut(s)	264, 911, 3043, 3483, 4053
FspBI	CTAG	5 cut(s)	1892, 2156, 2508, 3591, 3716
FspI	TGCGCA	1 cut(s)	1304
GlaI	GCGC	6 cut(s)	175, 673, 1304, 1799, 2988, 4376
GsuI	CTGGAG	1 cut(s)	424
HaeII	RGCGCY	2 cut(s)	675, 4378
HaeIII	GGCC	5 cut(s)	1503, 2090, 2526, 3322, 4203
HapII	CCGG	4 cut(s)	405, 522, 1524, 4217
HgaI	GACGC	1 cut(s)	3176
HhaI	GCGC	6 cut(s)	176, 674, 1305, 1800, 2989, 4377
Hin1I	GRCGYC	1 cut(s)	3187
Hin6I	GCGC	6 cut(s)	174, 672, 1303, 1798, 2987, 4375
HinP1I	GCGC	6 cut(s)	174, 672, 1303, 1798, 2987, 4375
HincII	GTYRAC	2 cut(s)	1099, 3954
HindII	GTYRAC	2 cut(s)	1099, 3954
HindIII	AAGCTT	3 cut(s)	1630, 2372, 2839
HpaII	CCGG	4 cut(s)	405, 522, 1524, 4217
HphI	GGTGA	1 cut(s)	3981
Hpy166II	GTNNAC	8 cut(s)	1099, 1763, 2391, 3102, 3954, 3989, 4088, 4492
Hpy8I	GTNNAC	8 cut(s)	1099, 1763, 2391, 3102, 3954, 3989, 4088, 4492
HpyAV	CCTTC	8 cut(s)	66, 987, 1196, 1372, 2954, 3262, 3286, 3457
HpyCH4III	ACNGT	8 cut(s)	400, 436, 800, 1370, 1531, 2548, 3137, 3814
HpyCH4IV	ACGT	6 cut(s)	924, 945, 1261, 1704, 3211, 4084
HpySE526I	ACGT	6 cut(s)	924, 945, 1261, 1704, 3211, 4084
Hsp92I	GRCGYC	1 cut(s)	3187
HspAI	GCGC	6 cut(s)	174, 672, 1303, 1798, 2987, 4375
KpnI	GGTACC	1 cut(s)	3442
LmnI	GCTCC	7 cut(s)	369, 595, 815, 1974, 2913, 3046, 4457
MaeI	CTAG	5 cut(s)	1892, 2156, 2508, 3591, 3716
MaeII	ACGT	6 cut(s)	924, 945, 1261, 1704, 3211, 4084
MaeIII	GTNAC	5 cut(s)	744, 941, 2328, 3879, 4412
MbiI	CCGCTC	2 cut(s)	1588, 3301
MfeI	CAATTG	5 cut(s)	1715, 1923, 2298, 3446, 3600
MflI	RGATCY	4 cut(s)	973, 1858, 2566, 3109
MlsI	TGGCCA	1 cut(s)	4203
MluNI	TGGCCA	1 cut(s)	4203
MlyI	GAGTC	7 cut(s)	417, 1349, 2129, 3035, 4383, 4487, 4520
MmeI	TCCRAC	7 cut(s)	477, 521, 1059, 1296, 1620, 3623, 4501
Mox20I	TGGCCA	1 cut(s)	4203
Mph1103I	ATGCAT	4 cut(s)	394, 1049, 3349, 3360
MroXI	GAANNNNTTC	2 cut(s)	1549, 4110
MscI	TGGCCA	1 cut(s)	4203
MslI	CAYNNNNRTG	5 cut(s)	34, 1092, 1290, 2627, 3886
Msp20I	TGGCCA	1 cut(s)	4203
MspA1I	CMGCKG	2 cut(s)	2294, 2564
MspCI	CTTAAG	1 cut(s)	3820
MspI	CCGG	4 cut(s)	405, 522, 1524, 4217
MspR9I	CCNGG	6 cut(s)	367, 594, 624, 1278, 1879, 2087
MunI	CAATTG	5 cut(s)	1715, 1923, 2298, 3446, 3600
Mva1269I	GAATGC	1 cut(s)	1281
MvaI	CCWGG	6 cut(s)	367, 594, 624, 1278, 1879, 2087
MvnI	CGCG	1 cut(s)	1798
NcoI	CCATGG	1 cut(s)	3287
NdeI	CATATG	2 cut(s)	215, 3523
NheI	GCTAGC	1 cut(s)	2507
NmuCI	GTSAC	2 cut(s)	3879, 4412
NsbI	TGCGCA	1 cut(s)	1304
NsiI	ATGCAT	4 cut(s)	394, 1049, 3349, 3360
NspI	RCATGY	4 cut(s)	2970, 3092, 3394, 3725
OliI	CACNNNNGTG	1 cut(s)	2627
PaeR7I	CTCGAG	3 cut(s)	4046, 4391, 4508
PagI	TCATGA	1 cut(s)	2776
PciI	ACATGT	3 cut(s)	3088, 3390, 3721
PcsI	WCGNNNNNNNCGW	1 cut(s)	192
PctI	GAATGC	1 cut(s)	1281
PdmI	GAANNNNTTC	2 cut(s)	1549, 4110
Pfl23II	CGTACG	1 cut(s)	4118
PflFI	GACNNNGTC	1 cut(s)	1339
PflMI	CCANNNNNTGG	4 cut(s)	1634, 1717, 2870, 3254
PfoI	TCCNGGA	2 cut(s)	365, 622
PleI	GAGTC	7 cut(s)	416, 1348, 2128, 3034, 4383, 4487, 4519
PpsI	GAGTC	7 cut(s)	416, 1348, 2128, 3034, 4383, 4487, 4519
Ppu21I	YACGTR	2 cut(s)	925, 1262
PpuMI	RGGWCCY	1 cut(s)	2755
PscI	ACATGT	3 cut(s)	3088, 3390, 3721
PshBI	ATTAAT	2 cut(s)	4029, 4326
PsiI	TTATAA	2 cut(s)	1323, 3194
Psp124BI	GAGCTC	4 cut(s)	264, 911, 3043, 4053
Psp5II	RGGWCCY	1 cut(s)	2755
Psp6I	CCWGG	6 cut(s)	365, 592, 622, 1276, 1877, 2085
PspGI	CCWGG	6 cut(s)	365, 592, 622, 1276, 1877, 2085
PspLI	CGTACG	1 cut(s)	4118
PspPI	GGNCC	5 cut(s)	491, 1865, 2083, 2524, 2755
PspPPI	RGGWCCY	1 cut(s)	2755
PspXI	VCTCGAGB	2 cut(s)	4391, 4508
PstNI	CAGNNNCTG	4 cut(s)	818, 1066, 1733, 2897
PsuI	RGATCY	4 cut(s)	973, 1858, 2566, 3109
PsyI	GACNNNGTC	1 cut(s)	1339
PvuII	CAGCTG	1 cut(s)	2294
RsaI	GTAC	6 cut(s)	887, 3134, 3395, 3440, 4120, 4286
RsaNI	GTAC	6 cut(s)	886, 3133, 3394, 3439, 4119, 4285
RseI	CAYNNNNRTG	5 cut(s)	34, 1092, 1290, 2627, 3886
SacI	GAGCTC	4 cut(s)	264, 911, 3043, 4053
Sau96I	GGNCC	5 cut(s)	491, 1865, 2083, 2524, 2755
SchI	GAGTC	7 cut(s)	417, 1349, 2129, 3035, 4383, 4487, 4520
ScrFI	CCNGG	6 cut(s)	367, 594, 624, 1278, 1879, 2087
SfcI	CTRYAG	2 cut(s)	2049, 2676
Sfr274I	CTCGAG	3 cut(s)	4046, 4391, 4508
SinI	GGWCC	4 cut(s)	491, 1865, 2083, 2755
SlaI	CTCGAG	3 cut(s)	4046, 4391, 4508
SmiMI	CAYNNNNRTG	5 cut(s)	34, 1092, 1290, 2627, 3886
SnaBI	TACGTA	2 cut(s)	925, 1262
SspI	AATATT	4 cut(s)	1209, 1434, 2953, 3704
SspMI	CTAG	5 cut(s)	1892, 2156, 2508, 3591, 3716
SstI	GAGCTC	4 cut(s)	264, 911, 3043, 4053
StyD4I	CCNGG	6 cut(s)	365, 592, 622, 1276, 1877, 2085
StyI	CCWWGG	1 cut(s)	3287
TaaI	ACNGT	8 cut(s)	400, 436, 800, 1370, 1531, 2548, 3137, 3814
TaiI	ACGT	6 cut(s)	927, 948, 1264, 1707, 3214, 4087
TaqI	TCGA	9 cut(s)	249, 860, 968, 2877, 4047, 4134, 4252, 4392, 4509
TaqII	GACCGA	1 cut(s)	4077
TatI	WGTACW	2 cut(s)	885, 4284
TauI	GCSGC	3 cut(s)	71, 509, 1841
TseFI	GTSAC	2 cut(s)	3879, 4412
Tsp45I	GTSAC	2 cut(s)	3879, 4412
TspGWI	ACGGA	4 cut(s)	319, 504, 749, 4136
Tth111I	GACNNNGTC	1 cut(s)	1339
Van91I	CCANNNNNTGG	4 cut(s)	1634, 1717, 2870, 3254
Vha464I	CTTAAG	1 cut(s)	3820
VneI	GTGCAC	1 cut(s)	1761
VpaK11BI	GGWCC	4 cut(s)	491, 1865, 2083, 2755
VspI	ATTAAT	2 cut(s)	4029, 4326
XapI	RAATTY	8 cut(s)	278, 1383, 1449, 1906, 1916, 2039, 2501, 4405
XceI	RCATGY	4 cut(s)	2970, 3092, 3394, 3725
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3525, 4347
XhoI	CTCGAG	3 cut(s)	4046, 4391, 4508
XmiI	GTMKAC	1 cut(s)	4087
XmnI	GAANNNNTTC	2 cut(s)	1549, 4110
XspI	CTAG	5 cut(s)	1892, 2156, 2508, 3591, 3716
Zsp2I	ATGCAT	4 cut(s)	394, 1049, 3349, 3360

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.