Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0002601

Physical Location & Seq

Genomic Coordinates Forward (+)

44301007 .. 44316845

Gene Structure 15839 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00116920.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4440 bp

ATGGAAGTGCCAATCCTCGGCAGCGATTCACTGAAATGGATTGAGTTCCCTGTTTCTTCTTCTAATGCCGCCACCGCCGATGCTGATACTTGTGCCTCTCTCACTCACGATTGCGCTTCTTCCATCGCCATTGGCGACCCTCCTACACATCTCATATGGAGAATCCACAAGCATCTCCCTCACTCTCTCGAATTACTTGAGCTCTCTGCTAACAAAGAATTTCCAAAACTCGGACTGCGAATCACTTTTCCACAAGCACTCTCTTCCTCCGTTTTTCTTTGCAAAAATGAGATTGATGTCGGCTCCAGGAGCCACCCCTATTTGCTCTATGCATTGACCGTCGCCGGAGTCGCTTACCTTTTGAGACTTGGAACCGTCTCTAGTTATGCTTCCAGCTCTGTTATTAGAGAGTTTAGCTTGCATCCCCACGGACCTATAACCAGTGCTGCAGCAACGCCAACCGGATGTCTTGTTGTAGGTCGGAATGACGGCTCACTCGCTTGCTTTCAACTCACACTTGAATCCACTGCTCCTGGTTTTCTGCAAGAGCTACAGGATGATCCAGGAATCGGTAGTTTGTGGGGTTTCATGTCAAGGGGTAGGACGTTGGGCGCTGTGCAGGACTTGGCTATATCTATGGTTCATGGGAAACCACTTATATTCGTTATTCATACGGATGGGGTGTTAAGAGTATGGGATATATCATTTCATAGCAGGCTCTTTTCCCATAAGATGAACGGAGCTGCATTGGTAAGGTTGTGGGTTGGTCAAGCTGACAATGATTCCAGCATAATTCCTTTGGCAATCTTGTACAGACATAATTCGGAAATGAGCTCAGAGACGATCTACGTATATAGCCTCCATTGTAATGTGGGAGACAGAATTGTTTTATCGTTGGATCCTTCAGAACAAATTATTCCTATTGAGGAGGATGGATGGATTGATGTCAAACTTATTTCCAACAAGATATGCATATTGAAAAATCAGGGACTAGTGCTTCACAAGTTGTTGCATACAAATGTCAACATGGTAGATGCAGTTTGTTATGCTTTGCAAGAGGAATTCGTTGCAGATCAACTCTTTCAAAGTTCTGAACATTCTTCGGATGACCTTCTTTTGATAACTCATTCAATATTTTCATCTTCAAAGGATCATATTCTACCCGTTGTATCTTCTATTTTCTTACGTAGGCTGCTTCTTCCTGGCATTCATCATAGTGCTGCTTTGCGCACAACTTTGTTGGATTATAATAGGCACTGGACTGAGTCAGATTTCCACTCCTTAACTGCTGACGGGTTGAAGAAGGAAATTCTTTCCCTGATTGAACATGAGGGTCTGACTGGTAATTCATCGTCAATATTTTGCTGCTGGAAAATTTTTTGTGCTCGCTATTTCCAAAACTGGTGCAAGAGCAATGCACCATGCGGCCTGCTTGTTGATTCCTCAACCGGCACTGTTGGTTTAATTAGAAAGAGTTCAGTATCTCTTTTTCGTTCTTTGGAGGATATTGAGCGGGTTAATGATGGCTCTTTGGATGAACTTGGCAATTTTCCAAGCTTTGGGTTGGATTCATTTGATGAGGCTCTTGATTGTGAGCTACTTGCTGAAATGCTCAGATGTGTCATTAACGTCAGCCAGCAATTGGGCAAAACAGCTTCTGCTATATTTTATGAATCACTCATTAGTGCACCACCAGTTATCTCATCTGAAGAAATTATTCCGCGCCTATTGAAGATATTAGAAACTGGATATAGTTCAACAGCTGCAGTGCTTCATATATCAGATCTTGGACCTGATGTTGCCTGGGAGAAAAATCTAGCAGATCACAAAAATTTGAGGAAATTTTCAATTGACATGATGTTGTCTCTTCATGCTTTGCACAAGAAATCTGGCACATGGAGCCGTATTTTGAGTGTGATTGAGAACTATCTAAAGTATCTCATACCTCGGAAAATTATACAAAAATTTGATGCTGAAGTAGCATTGGATATCAATGCCTACATCTTGGTCCAGGCTATGTCTCAAGTTGCAAAGGTTATGTTTGAGTCTGCACTGGATATTCATCTGTTTCTAAGCTATCTAGTGAGCATTAGTGGGCAGATCAATATGCTACATGATGATATATCCAAAATACAGCTTCAGTTGGTTCCAATGATTCAAGAAATCATTTATGAGTGGCTTCTCTTACATTTCTTTGCCACCACACCATCTGAATCAGCTGCAATTGAAGATTTCAGCTCTCAACTTTCATTGTTACAAATTGATAGCAACACAGGCAGAAGATCATGGAATGAGAAGCTTGGGAAGTGCGAGTTCACTCTGGCTTTTATATTCTTACTTAATGTTAGAAGTTCCTCCAAAGACCAGAGCCACTTTTCTCCAAGAAGCATACCAAACGTTCAGGACATAATTGATTCAATACGAGAATTTGCTAGCTGGGTCATGTGGGGCCGGAATGGGGAATCCCTTACTGTCCTGAGGCGTGCAGCGGATCTTGCATTGATCCTGCTAAGGCACGGGCAGTATGATGCTGTTGAGCACCTACTCACTATTGTGGAGGCACATTTACAAAAGGAGAAGACTTCTCATCGTATTCAAGCTACAGATGGCGGATGGTGCATACTGCATCATCTTTTAGGATGCTGTTTCCTTGCTCAAGCACATCGTGGATTACATGGGGTCCTGAAAGAGAGGAAAGTTCATGAAGCTGTTCGTTGTTTCTTCAGAGCTGCATCTGGAAACGGCTCAGCGCAGGATTTACAAAGCTTGCCTCAGGAAGCGGGCTTGCCACATCTTGGTATCGATGGTAGTGTATCAGATGCTGCATGGAGGCTCCATTACTATCAGTGGGCAATGCAGATATTTGAGCAATATAATATTAGTGAAGGTGCATGTCAGTTTGCTCTCGCTGCGCTTGAGCAAGTCGAGGAAGCTTATAGTGCAAACAATGAGTCTCATGACAGAGCTCCATTTGATGAATCAGTATCTACCATCAAAGGAAGACTTTGGGCACATGTTTGCAAGTTTACTTTAGATCTCAATCTCTTTTATGATGCGTATTGTGCAATAATTTCAAATCCAGATGAGGAGAGCAAGCACATCTGGTTGAGGCGTCTTATAATTGTTCTTTATGAACGTGGTGCCATAAAGATTCTTTGTGGTGGTCAGCTACCATTCATTGGTCTTACAGAGAAGGTGGAACAAGAGCTTGCATGGAAGGCTGAGCGGTCAGACATTTTAGCAAAGCCAAACTTATATAAGTTGCTTTATGCATTTGAAATGCATCGGCATAACTGGCGAAAAGCAGCAAGCTACATGTACCTTTATTCTGCTCGTTTGAGAACTGAAACATCTCTGAAAGGGTACCAGCAATTGTGGCGAGCACTGAAGGAGATACTGAATGGGCTCTCTGCTGCTATCAACGCACTTCATCTTGTTCATCCAGCATATGCTTGGATTGATCCTCTCCTTGAGAGAAATGCTAATGGCAATGAGCAGTATCCAAGTAAAAAGGCTAGAATAACAATTGAAGACCAGTCAGCAAGCAATGATGTTGATCCTCAAAGTTGGAAATCTTTTATAGATATTCAGAAAATTGAAAACGAGTTTGTGCTTACTTCAGCAGAATATTTGCTTTCACTGGCACACGTCAAATGGACAAATACAGGAACCCAGAAAGCACCATTGGAGTTGGTTGATCTCTTGATCCAGACAAATCTATATGATATGGCATTTACAGTTCTCTTAAGATTCTTCAAAGGTTCAGAGTTAAAGAGGGGATTGGAAAGAGTTTTCTCTGCTATGTCACTAAAATGCTGTCCACATATAGTTAATTCTTCAAGGGTCGGGGATGATTCCACAACACGGGGTCTTCTGTTGACTTCTTCGAAGGATGAAGTGATCGTTCATGGTTCACCAGATACAAGTTCAGCAAATCAACAATCCAGCGGAATTAATCAATGGGGAACTCTCGAGCTTTATCTTGACCGATATAAAGTCTTCCATGCACGTCTACCATTTATTGTTGCTGAAACTCTTCTTCGTACGGATTCTCAAATCGAACTGCCTCTTTGGTTGGTTAAATTATTCAAGGTTGGCAGGAGGGAAAAGACATTGGGGATGACTGGCCAAGAATCAAGTCCGGCATTATTATTTCAACTATATGTTGATTATGGTCGATATAGAGAAGCTACGAATCTGTTGCTTGAGTACATAGGTTCTTTTGCATCCATGAGACCAGCCAACATCATGAATCGTAAAAGACCATTTGGAGTTTGGTTTCCATATACAACAATTCAGCGCCTCTGGTGCCAACTTGAGGAAATGATAAATTCAGGTCACATGGTAGATCAATGTAAGCAGCTCAAAGATTTGCTACATGGAGCTTTGCTGAAACATCTCCAACTGGTAAAAGTGGACTCGGAAGATGCAATTAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0008104 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016973 GO:0031090 GO:0031503 GO:0031965 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0034613 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

DUF5311 Nup160

Protein Analysis

1479

Amino Acids

166.16

Weight (kDa)

6.1

Isoelectric Point (pI)

43.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_Nup120_160	PF11715	51 - 504	1.9e-55	Nucleoporin Nup120/160, beta-propeller domain
NUP160_helical_2	PF17238	521 - 715	1.2e-90	NUP160, helical domain
TPR_NUP160_120_M	PF23354	894 - 1145	1.8e-34	NUP160/120 middle TPR
HEAT_Nup120	PF23300	895 - 1044	5.9e-13	Nucleoporin Nup120, HEAT repeats
TPR_Nup160_C	PF23347	1197 - 1477	5.5e-13	Nup160 C-terminal TPR

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002601)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33410 AT1G33410
fragaria_vesca	FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400
malus_domestica	MD00G1034800.v1.1 MD05G1063300.v1.1 MD05G1161100.v1.1 MD05G1162300.v1.1 MD05G1162400.v1.1 MD07G1280900.v1.1 MD08G1158600.v1.1 MD10G1009800.v1.1 MD10G1160000.v1.1
prunus_persica	Prupe.5G022000_v2.0.a1
pyrus_communis	pycom10g00600
rosa_chinensis	RchiOBHm_Chr2g0126921 RchiOBHm_Chr4g0402211
rosa_laevigata	RLG00000009106 RLG00000018928
rosa_multiflora	Rmu_sc0000007.1_g000030 Rmu_sc0001405.1_g000051
rosa_roxburghii	Rroxscaffold_1G00047890 Rroxscaffold_2G00116920 Rroxscaffold_5G00346940
rosa_rugosa	Rorug02G0270100 Rorug02G0270200
rosa_samantha	Rh2AG322500 Rh2BG330700 Rh2CG310600 Rh2DG350000 Rh4BG104100 Rh4CG117900 Rh4DG101500
rosa_wichuraiana	Rw2G026160 Rw4G008910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1248, 3119
Acc16I	TGCGCA	1 cut(s)	1229
Acc65I	GGTACC	1 cut(s)	3363
AccB1I	GGYRCC	3 cut(s)	3140, 3363, 4308
AccB7I	CCANNNNNTGG	4 cut(s)	1559, 1642, 2795, 3179
AccBSI	CCGCTC	2 cut(s)	1513, 3226
AccI	GTMKAC	1 cut(s)	4012
AccII	CGCG	1 cut(s)	1723
AclI	AACGTT	1 cut(s)	2397
AclWI	GGATC	9 cut(s)	554, 893, 906, 1158, 2497, 2499, 3455, 3551, 3700
AcoI	YGGCCR	1 cut(s)	4126
AcsI	RAATTY	9 cut(s)	218, 1061, 1308, 1374, 1831, 1841, 1964, 2426, 4330
AcuI	CTGAAG	7 cut(s)	888, 1728, 1995, 2123, 2707, 3407, 3603
AcyI	GRCGYC	1 cut(s)	3112
AdeI	CACNNNGTG	1 cut(s)	2666
AfaI	GTAC	5 cut(s)	812, 3320, 3365, 4045, 4211
AflII	CTTAAG	1 cut(s)	3745
AflIII	ACRYGT	3 cut(s)	3013, 3315, 3646
AhlI	ACTAGT	1 cut(s)	991
AjiI	CACGTC	2 cut(s)	3649, 4010
AjnI	CCWGG	6 cut(s)	305, 532, 562, 1201, 1802, 2010
AjuI	GAANNNNNNNTTGG	2 cut(s)	3668, 3700
AleI	CACNNNNGTG	1 cut(s)	2552
Alw21I	GWGCWC	7 cut(s)	204, 836, 1387, 1690, 2541, 2968, 3385
Alw26I	GTCTC	8 cut(s)	358, 382, 833, 870, 1869, 2025, 2958, 4228
Alw44I	GTGCAC	1 cut(s)	1686
AlwI	GGATC	9 cut(s)	554, 893, 906, 1158, 2497, 2499, 3455, 3551, 3700
AlwNI	CAGNNNCTG	2 cut(s)	1658, 2822
Ama87I	CYCGRG	1 cut(s)	3971
AoxI	GGCC	3 cut(s)	1426, 2449, 4126
ApaLI	GTGCAC	1 cut(s)	1686
ApoI	RAATTY	9 cut(s)	218, 1061, 1308, 1374, 1831, 1841, 1964, 2426, 4330
ArsI	GACNNNNNNTTYG	2 cut(s)	2936, 2968
AseI	ATTAAT	1 cut(s)	3954
Asp700I	GAANNNNTTC	4 cut(s)	1474, 1716, 2709, 4035
Asp718I	GGTACC	1 cut(s)	3363
AspLEI	GCGC	7 cut(s)	116, 614, 1230, 1725, 2752, 2914, 4302
AspS9I	GGNCC	5 cut(s)	431, 1790, 2008, 2449, 2680
AsuHPI	GGTGA	1 cut(s)	3906
AsuII	TTCGAA	1 cut(s)	3887
AsuNHI	GCTAGC	1 cut(s)	2432
AvaI	CYCGRG	1 cut(s)	3971
AvaII	GGWCC	4 cut(s)	431, 1790, 2008, 2680
AxyI	CCTNAGG	2 cut(s)	2477, 2772
BaeGI	GKGCMC	2 cut(s)	1690, 3013
BalI	TGGCCA	1 cut(s)	4128
BamHI	GGATCC	1 cut(s)	898
BanI	GGYRCC	3 cut(s)	3140, 3363, 4308
BanII	GRGCYC	4 cut(s)	204, 836, 2968, 3408
BbsI	GAAGAC	5 cut(s)	2585, 3007, 3537, 3863, 3991
Bbv12I	GWGCWC	7 cut(s)	204, 836, 1387, 1690, 2541, 2968, 3385
BceAI	ACGGC	3 cut(s)	505, 1887, 2758
BcgI	CGANNNNNNTGC	2 cut(s)	4183, 4217
BciT130I	CCWGG	6 cut(s)	307, 534, 564, 1203, 1804, 2012
BciVI	GTATCC	1 cut(s)	3510
BcoDI	GTCTC	8 cut(s)	358, 382, 833, 870, 1869, 2025, 2958, 4228
BcuI	ACTAGT	1 cut(s)	991
BfaI	CTAG	6 cut(s)	381, 992, 1817, 2081, 2433, 3516
BfmI	CTRYAG	4 cut(s)	447, 551, 1764, 2601
BfoI	RGCGCY	2 cut(s)	615, 4303
BfrI	CTTAAG	1 cut(s)	3745
BfuI	GTATCC	1 cut(s)	3510
BglII	AGATCT	2 cut(s)	1783, 3034
BlpI	GCTNAGC	2 cut(s)	2746, 3222
Bme1390I	CCNGG	6 cut(s)	307, 534, 564, 1203, 1804, 2012
Bme18I	GGWCC	4 cut(s)	431, 1790, 2008, 2680
BmeT110I	CYCGRG	1 cut(s)	3971
BmgBI	CACGTC	2 cut(s)	3649, 4010
BmgT120I	GGNCC	5 cut(s)	431, 1790, 2008, 2449, 2680
BmrFI	CCNGG	6 cut(s)	307, 534, 564, 1203, 1804, 2012
BmtI	GCTAGC	1 cut(s)	2436
BpiI	GAAGAC	5 cut(s)	2585, 3007, 3537, 3863, 3991
BpmI	CTGGAG	1 cut(s)	289
Bpu10I	CCTNAGC	1 cut(s)	2510
Bpu1102I	GCTNAGC	2 cut(s)	2746, 3222
Bpu14I	TTCGAA	1 cut(s)	3887
BpuEI	CTTGAG	7 cut(s)	218, 2007, 2640, 2936, 3491, 4226, 4337
Bsa29I	ATCGAT	1 cut(s)	2802
BsaAI	YACGTR	2 cut(s)	850, 1187
BsaBI	GATNNNNATC	3 cut(s)	2061, 3039, 3555
BsaHI	GRCGYC	1 cut(s)	3112
BsaI	GGTCTC	1 cut(s)	4228
BsaJI	CCNNGG	4 cut(s)	16, 427, 1803, 1946
BsaWI	WCCGGW	1 cut(s)	461
Bse118I	RCCGGY	1 cut(s)	1448
Bse21I	CCTNAGG	2 cut(s)	2477, 2772
Bse3DI	GCAATG	4 cut(s)	1420, 2859, 3496, 3553
Bse8I	GATNNNNATC	3 cut(s)	2061, 3039, 3555
BseBI	CCWGG	6 cut(s)	307, 534, 564, 1203, 1804, 2012
BseCI	ATCGAT	1 cut(s)	2802
BseDI	CCNNGG	4 cut(s)	16, 427, 1803, 1946
BseJI	GATNNNNATC	3 cut(s)	2061, 3039, 3555
BseMI	GCAATG	4 cut(s)	1420, 2859, 3496, 3553
BseMII	CTCAG	7 cut(s)	849, 1254, 1627, 2468, 2760, 2786, 3213
BseRI	GAGGAG	2 cut(s)	941, 3101
BseSI	GKGCMC	2 cut(s)	1690, 3013
BseYI	CCCAGC	1 cut(s)	2436
BsgI	GTGCAG	3 cut(s)	638, 2034, 2505
Bsh1236I	CGCG	1 cut(s)	1723
BshFI	GGCC	3 cut(s)	1428, 2451, 4128
BshNI	GGYRCC	3 cut(s)	3140, 3363, 4308
BshVI	ATCGAT	1 cut(s)	2802
BsiHKAI	GWGCWC	7 cut(s)	204, 836, 1387, 1690, 2541, 2968, 3385
BsiHKCI	CYCGRG	1 cut(s)	3971
BsiSI	CCGG	5 cut(s)	345, 462, 1449, 2452, 4142
BsiWI	CGTACG	1 cut(s)	4043
BslFI	GGGAC	1 cut(s)	1002
BsmAI	GTCTC	8 cut(s)	358, 382, 833, 870, 1869, 2025, 2958, 4228
BsmBI	CGTCTC	2 cut(s)	382, 833
BsmFI	GGGAC	1 cut(s)	1002
BsmI	GAATGC	1 cut(s)	1206
BsnI	GGCC	3 cut(s)	1428, 2451, 4128
Bso31I	GGTCTC	1 cut(s)	4228
BsoBI	CYCGRG	1 cut(s)	3971
Bsp119I	TTCGAA	1 cut(s)	3887
Bsp1286I	GDGCHC	9 cut(s)	204, 836, 1387, 1690, 2541, 2968, 3013, 3385, 3408
Bsp1407I	TGTACA	1 cut(s)	810
Bsp1720I	GCTNAGC	2 cut(s)	2746, 3222
BspANI	GGCC	3 cut(s)	1428, 2451, 4128
BspCNI	CTCAG	7 cut(s)	848, 1255, 1626, 2469, 2759, 2785, 3214
BspDI	ATCGAT	1 cut(s)	2802
BspFNI	CGCG	1 cut(s)	1723
BspHI	TCATGA	3 cut(s)	2701, 2956, 4248
BspMAI	CTGCAG	2 cut(s)	451, 1768
BspOI	GCTAGC	1 cut(s)	2436
BspPI	GGATC	9 cut(s)	554, 893, 906, 1158, 2497, 2499, 3455, 3551, 3700
BspT104I	TTCGAA	1 cut(s)	3887
BspT107I	GGYRCC	3 cut(s)	3140, 3363, 4308
BspTI	CTTAAG	1 cut(s)	3745
BspTNI	GGTCTC	1 cut(s)	4228
BsrBI	CCGCTC	2 cut(s)	1513, 3226
BsrDI	GCAATG	4 cut(s)	1420, 2859, 3496, 3553
BsrFI	RCCGGY	1 cut(s)	1448
BsrGI	TGTACA	1 cut(s)	810
BssAI	RCCGGY	1 cut(s)	1448
BssECI	CCNNGG	4 cut(s)	16, 427, 1803, 1946
BssNI	GRCGYC	1 cut(s)	3112
Bst2UI	CCWGG	6 cut(s)	307, 534, 564, 1203, 1804, 2012
Bst4CI	ACNGT	5 cut(s)	340, 376, 1456, 2473, 3739
Bst6I	CTCTTC	3 cut(s)	268, 1872, 4041
BstACI	GRCGYC	1 cut(s)	3112
BstAFI	CTTAAG	1 cut(s)	3745
BstAUI	TGTACA	1 cut(s)	810
BstBAI	YACGTR	2 cut(s)	850, 1187
BstBI	TTCGAA	1 cut(s)	3887
BstDEI	CTNAG	9 cut(s)	835, 1263, 1613, 2072, 2477, 2510, 2746, 2772, 3222
BstDSI	CCRYGG	1 cut(s)	427
BstFNI	CGCG	1 cut(s)	1723
BstH2I	RGCGCY	2 cut(s)	615, 4303
BstHHI	GCGC	7 cut(s)	116, 614, 1230, 1725, 2752, 2914, 4302
BstMAI	GTCTC	8 cut(s)	358, 382, 833, 870, 1869, 2025, 2958, 4228
BstNI	CCWGG	6 cut(s)	307, 534, 564, 1203, 1804, 2012
BstNSI	RCATGY	3 cut(s)	2895, 3017, 3319
BstSCI	CCNGG	6 cut(s)	305, 532, 562, 1201, 1802, 2010
BstSFI	CTRYAG	4 cut(s)	447, 551, 1764, 2601
BstSLI	GKGCMC	2 cut(s)	1690, 3013
BstSNI	TACGTA	2 cut(s)	850, 1187
BstUI	CGCG	1 cut(s)	1723
BstV2I	GAAGAC	5 cut(s)	2585, 3007, 3537, 3863, 3991
BstX2I	RGATCY	4 cut(s)	898, 1783, 2491, 3034
BstYI	RGATCY	4 cut(s)	898, 1783, 2491, 3034
Bsu15I	ATCGAT	1 cut(s)	2802
Bsu36I	CCTNAGG	2 cut(s)	2477, 2772
BsuI	GTATCC	1 cut(s)	3510
BsuRI	GGCC	3 cut(s)	1428, 2451, 4128
BsuTUI	ATCGAT	1 cut(s)	2802
BtgI	CCRYGG	1 cut(s)	427
BtgZI	GCGATG	1 cut(s)	109
BtrI	CACGTC	2 cut(s)	3649, 4010
BtsI	GCAGTG	2 cut(s)	525, 1773
CaiI	CAGNNNCTG	2 cut(s)	1658, 2822
CciI	TCATGA	3 cut(s)	2701, 2956, 4248
CfoI	GCGC	7 cut(s)	116, 614, 1230, 1725, 2752, 2914, 4302
Cfr10I	RCCGGY	1 cut(s)	1448
Cfr13I	GGNCC	5 cut(s)	431, 1790, 2008, 2449, 2680
ClaI	ATCGAT	1 cut(s)	2802
CseI	GACGC	1 cut(s)	3101
Csp6I	GTAC	5 cut(s)	811, 3319, 3364, 4044, 4210
CviQI	GTAC	5 cut(s)	811, 3319, 3364, 4044, 4210
DdeI	CTNAG	9 cut(s)	835, 1263, 1613, 2072, 2477, 2510, 2746, 2772, 3222
DraIII	CACNNNGTG	1 cut(s)	2666
EaeI	YGGCCR	1 cut(s)	4126
Eam1104I	CTCTTC	3 cut(s)	268, 1872, 4041
EarI	CTCTTC	3 cut(s)	268, 1872, 4041
EciI	GGCGGA	1 cut(s)	2625
Ecl136II	GAGCTC	3 cut(s)	202, 834, 2966
Eco105I	TACGTA	2 cut(s)	850, 1187
Eco24I	GRGCYC	4 cut(s)	204, 836, 2968, 3408
Eco31I	GGTCTC	1 cut(s)	4228
Eco32I	GATATC	1 cut(s)	1990
Eco47I	GGWCC	4 cut(s)	431, 1790, 2008, 2680
Eco53kI	GAGCTC	3 cut(s)	202, 834, 2966
Eco57I	CTGAAG	7 cut(s)	888, 1728, 1995, 2123, 2707, 3407, 3603
Eco81I	CCTNAGG	2 cut(s)	2477, 2772
Eco88I	CYCGRG	1 cut(s)	3971
EcoICRI	GAGCTC	3 cut(s)	202, 834, 2966
EcoO109I	RGGNCCY	1 cut(s)	2680
EcoRI	GAATTC	1 cut(s)	1061
EcoRII	CCWGG	6 cut(s)	305, 532, 562, 1201, 1802, 2010
EcoRV	GATATC	1 cut(s)	1990
EcoT22I	ATGCAT	4 cut(s)	334, 974, 3274, 3285
EcoT38I	GRGCYC	4 cut(s)	204, 836, 2968, 3408
Esp3I	CGTCTC	2 cut(s)	382, 833
FalI	AAGNNNNNCTT	4 cut(s)	2988, 3020, 3236, 3268
FaqI	GGGAC	1 cut(s)	1002
FauI	CCCGC	2 cut(s)	1506, 2773
FauNDI	CATATG	2 cut(s)	155, 3448
FblI	GTMKAC	1 cut(s)	4012
FriOI	GRGCYC	4 cut(s)	204, 836, 2968, 3408
FspBI	CTAG	6 cut(s)	381, 992, 1817, 2081, 2433, 3516
FspI	TGCGCA	1 cut(s)	1229
GlaI	GCGC	7 cut(s)	115, 613, 1229, 1724, 2751, 2913, 4301
GsaI	CCCAGC	1 cut(s)	2440
GsuI	CTGGAG	1 cut(s)	289
HaeII	RGCGCY	2 cut(s)	615, 4303
HaeIII	GGCC	3 cut(s)	1428, 2451, 4128
HapII	CCGG	5 cut(s)	345, 462, 1449, 2452, 4142
HgaI	GACGC	1 cut(s)	3101
HhaI	GCGC	7 cut(s)	116, 614, 1230, 1725, 2752, 2914, 4302
Hin1I	GRCGYC	1 cut(s)	3112
Hin6I	GCGC	7 cut(s)	114, 612, 1228, 1723, 2750, 2912, 4300
HinP1I	GCGC	7 cut(s)	114, 612, 1228, 1723, 2750, 2912, 4300
HincII	GTYRAC	2 cut(s)	1024, 3879
HindII	GTYRAC	2 cut(s)	1024, 3879
HindIII	AAGCTT	4 cut(s)	1555, 2297, 2764, 2931
HpaII	CCGG	5 cut(s)	345, 462, 1449, 2452, 4142
HphI	GGTGA	1 cut(s)	3906
Hpy166II	GTNNAC	9 cut(s)	1024, 1688, 2316, 3027, 3821, 3879, 3914, 4013, 4417
Hpy8I	GTNNAC	9 cut(s)	1024, 1688, 2316, 3027, 3821, 3879, 3914, 4013, 4417
Hpy99I	CGWCG	1 cut(s)	344
HpyAV	CCTTC	8 cut(s)	912, 1121, 1297, 2879, 3187, 3211, 3382, 3883
HpyCH4III	ACNGT	5 cut(s)	340, 376, 1456, 2473, 3739
HpyCH4IV	ACGT	8 cut(s)	605, 849, 1186, 1629, 2397, 3136, 3648, 4009
HpyF3I	CTNAG	9 cut(s)	835, 1263, 1613, 2072, 2477, 2510, 2746, 2772, 3222
HpySE526I	ACGT	8 cut(s)	605, 849, 1186, 1629, 2397, 3136, 3648, 4009
Hsp92I	GRCGYC	1 cut(s)	3112
HspAI	GCGC	7 cut(s)	114, 612, 1228, 1723, 2750, 2912, 4300
KpnI	GGTACC	1 cut(s)	3367
LmnI	GCTCC	8 cut(s)	308, 309, 535, 740, 1899, 2838, 2971, 4382
MaeI	CTAG	6 cut(s)	381, 992, 1817, 2081, 2433, 3516
MaeII	ACGT	8 cut(s)	605, 849, 1186, 1629, 2397, 3136, 3648, 4009
MaeIII	GTNAC	3 cut(s)	2253, 3804, 4337
MbiI	CCGCTC	2 cut(s)	1513, 3226
MfeI	CAATTG	5 cut(s)	1640, 1848, 2223, 3371, 3525
MflI	RGATCY	4 cut(s)	898, 1783, 2491, 3034
MhlI	GDGCHC	9 cut(s)	204, 836, 1387, 1690, 2541, 2968, 3013, 3385, 3408
MlsI	TGGCCA	1 cut(s)	4128
MluNI	TGGCCA	1 cut(s)	4128
MlyI	GAGTC	5 cut(s)	357, 1274, 2054, 2960, 4412
MmeI	TCCRAC	7 cut(s)	461, 876, 984, 1221, 1545, 3548, 4426
Mox20I	TGGCCA	1 cut(s)	4128
Mph1103I	ATGCAT	4 cut(s)	334, 974, 3274, 3285
MroXI	GAANNNNTTC	4 cut(s)	1474, 1716, 2709, 4035
MscI	TGGCCA	1 cut(s)	4128
MslI	CAYNNNNRTG	7 cut(s)	34, 675, 867, 1017, 1215, 2552, 3811
Msp20I	TGGCCA	1 cut(s)	4128
MspA1I	CMGCKG	4 cut(s)	1763, 2219, 2489, 3948
MspCI	CTTAAG	1 cut(s)	3745
MspI	CCGG	5 cut(s)	345, 462, 1449, 2452, 4142
MspR9I	CCNGG	6 cut(s)	307, 534, 564, 1203, 1804, 2012
MunI	CAATTG	5 cut(s)	1640, 1848, 2223, 3371, 3525
Mva1269I	GAATGC	1 cut(s)	1206
MvaI	CCWGG	6 cut(s)	307, 534, 564, 1203, 1804, 2012
MvnI	CGCG	1 cut(s)	1723
NdeI	CATATG	2 cut(s)	155, 3448
NheI	GCTAGC	1 cut(s)	2432
NmuCI	GTSAC	2 cut(s)	3804, 4337
NsbI	TGCGCA	1 cut(s)	1229
NsiI	ATGCAT	4 cut(s)	334, 974, 3274, 3285
NspI	RCATGY	3 cut(s)	2895, 3017, 3319
NspV	TTCGAA	1 cut(s)	3887
OliI	CACNNNNGTG	1 cut(s)	2552
PaeR7I	CTCGAG	1 cut(s)	3971
PagI	TCATGA	3 cut(s)	2701, 2956, 4248
PciI	ACATGT	2 cut(s)	3013, 3315
PcsI	WCGNNNNNNNCGW	1 cut(s)	132
PctI	GAATGC	1 cut(s)	1206
PdmI	GAANNNNTTC	4 cut(s)	1474, 1716, 2709, 4035
Pfl23II	CGTACG	1 cut(s)	4043
PflFI	GACNNNGTC	1 cut(s)	1264
PflMI	CCANNNNNTGG	4 cut(s)	1559, 1642, 2795, 3179
PfoI	TCCNGGA	2 cut(s)	305, 562
PleI	GAGTC	5 cut(s)	356, 1273, 2053, 2959, 4412
PpsI	GAGTC	5 cut(s)	356, 1273, 2053, 2959, 4412
Ppu21I	YACGTR	2 cut(s)	850, 1187
PpuMI	RGGWCCY	1 cut(s)	2680
PscI	ACATGT	2 cut(s)	3013, 3315
PshBI	ATTAAT	1 cut(s)	3954
PsiI	TTATAA	2 cut(s)	1248, 3119
Psp124BI	GAGCTC	3 cut(s)	204, 836, 2968
Psp1406I	AACGTT	1 cut(s)	2397
Psp5II	RGGWCCY	1 cut(s)	2680
Psp6I	CCWGG	6 cut(s)	305, 532, 562, 1201, 1802, 2010
PspFI	CCCAGC	1 cut(s)	2436
PspGI	CCWGG	6 cut(s)	305, 532, 562, 1201, 1802, 2010
PspLI	CGTACG	1 cut(s)	4043
PspPI	GGNCC	5 cut(s)	431, 1790, 2008, 2449, 2680
PspPPI	RGGWCCY	1 cut(s)	2680
PstI	CTGCAG	2 cut(s)	451, 1768
PstNI	CAGNNNCTG	2 cut(s)	1658, 2822
PsuI	RGATCY	4 cut(s)	898, 1783, 2491, 3034
PsyI	GACNNNGTC	1 cut(s)	1264
PvuII	CAGCTG	2 cut(s)	1763, 2219
RsaI	GTAC	5 cut(s)	812, 3320, 3365, 4045, 4211
RsaNI	GTAC	5 cut(s)	811, 3319, 3364, 4044, 4210
RseI	CAYNNNNRTG	7 cut(s)	34, 675, 867, 1017, 1215, 2552, 3811
SacI	GAGCTC	3 cut(s)	204, 836, 2968
Sau96I	GGNCC	5 cut(s)	431, 1790, 2008, 2449, 2680
SchI	GAGTC	5 cut(s)	357, 1274, 2054, 2960, 4412
ScrFI	CCNGG	6 cut(s)	307, 534, 564, 1203, 1804, 2012
SduI	GDGCHC	9 cut(s)	204, 836, 1387, 1690, 2541, 2968, 3013, 3385, 3408
SfcI	CTRYAG	4 cut(s)	447, 551, 1764, 2601
Sfr274I	CTCGAG	1 cut(s)	3971
SfuI	TTCGAA	1 cut(s)	3887
SinI	GGWCC	4 cut(s)	431, 1790, 2008, 2680
SlaI	CTCGAG	1 cut(s)	3971
SmiMI	CAYNNNNRTG	7 cut(s)	34, 675, 867, 1017, 1215, 2552, 3811
SmlI	CTYRAG	9 cut(s)	197, 2022, 2655, 2915, 3470, 3745, 3971, 4205, 4316
SmoI	CTYRAG	9 cut(s)	197, 2022, 2655, 2915, 3470, 3745, 3971, 4205, 4316
SnaBI	TACGTA	2 cut(s)	850, 1187
SpeI	ACTAGT	1 cut(s)	991
SspI	AATATT	4 cut(s)	1134, 1359, 2878, 3629
SspMI	CTAG	6 cut(s)	381, 992, 1817, 2081, 2433, 3516
SstI	GAGCTC	3 cut(s)	204, 836, 2968
StyD4I	CCNGG	6 cut(s)	305, 532, 562, 1201, 1802, 2010
TaaI	ACNGT	5 cut(s)	340, 376, 1456, 2473, 3739
TaiI	ACGT	8 cut(s)	608, 852, 1189, 1632, 2400, 3139, 3651, 4012
TaqI	TCGA	7 cut(s)	189, 2802, 2925, 3887, 3972, 4059, 4177
TaqII	GACCGA	1 cut(s)	4002
TatI	WGTACW	2 cut(s)	810, 4209
TauI	GCSGC	2 cut(s)	71, 1428
TseFI	GTSAC	2 cut(s)	3804, 4337
Tsp45I	GTSAC	2 cut(s)	3804, 4337
TspGWI	ACGGA	5 cut(s)	259, 444, 689, 753, 4061
Tth111I	GACNNNGTC	1 cut(s)	1264
Van91I	CCANNNNNTGG	4 cut(s)	1559, 1642, 2795, 3179
Vha464I	CTTAAG	1 cut(s)	3745
VneI	GTGCAC	1 cut(s)	1686
VpaK11BI	GGWCC	4 cut(s)	431, 1790, 2008, 2680
VspI	ATTAAT	1 cut(s)	3954
XapI	RAATTY	9 cut(s)	218, 1061, 1308, 1374, 1831, 1841, 1964, 2426, 4330
XceI	RCATGY	3 cut(s)	2895, 3017, 3319
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3450, 4272
XhoI	CTCGAG	1 cut(s)	3971
XmiI	GTMKAC	1 cut(s)	4012
XmnI	GAANNNNTTC	4 cut(s)	1474, 1716, 2709, 4035
XspI	CTAG	6 cut(s)	381, 992, 1817, 2081, 2433, 3516
Zsp2I	ATGCAT	4 cut(s)	334, 974, 3274, 3285

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.