Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0002601

Physical Location & Seq

Genomic Coordinates Reverse (-)

19759981 .. 19802862

Gene Structure 42882 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G008910.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4950 bp

AAGAATGAGAGCTTGGGTTTGTACCATTACCAGGTCGAATTAGAGATGGACTCAAAGTCCGAAATCAACTTGGAGAGGCCAACACATGCATGGGTCTCCAACGGAAAGTCTCTGAGTACCCGAAGCTTGAAACTTGGCCAGTTGCTTTTCTCTGTGAAGAACCAGTTTACAGCAATTTTGGAAAGAGTGGAGCTGTGTGCATCGCGGAGCCATCCAATCCGAAGATTGACACATTGTGGGTTCACGGAGATCCTGAAAACCTACCCCCGCTCCAGCTGCCCTGAGGGAGTAGTTGTGCTTGTTGTCAGCTTATACCTCATCAGTTTTCTTAGCATTTTGGTTTTCACTCTGTGCGGATATTCTTTCTCTGCTCATGGTCAAAGAAGAGAAATCAGGGTCTCCGAGTTAGCCAAGATTGTGGGCATGGAGGAATCGGTAGGCATGGAAGTGCCAATCCTTGGCAGCGATTCAGTGAAATGGATCGAGTTCTCTATTCTTTCATCTAATGAATTGGCCGCCACCGCCGATGCTGATACTTGTGCCCCTCTCACTCACGATTGCGCTTCTTCCATCGCCATTGGAGACCCTCCTACATATCTCATATGGAGAATCCACAAGGATTTCCCCCGTACTCTCGAATTACTCGAGCTCTCTGCTAAGAAAGAATTTCCAAAAGTCGGACTGCGAATCACTTTTCCAGAATCACTCTCTTCTTCAGTTTTTCTTTGCAAAAATGAGATTGATATCGGTTCCGGGAGCCACCCCTATTTGCTTTATGCATTGACCGTGGCCGGAGTCGCTTACCTTTTGAAACTTGGGACCACCTCTAGCTATGCACCCTGCTCTGTTATTGGAGAGTTTAACTTGCATCCCTACGGACCAATTAGCAGTGCCACAGCAACGCCGACCGGATGTCTTGTTGTAGGTCGGAATGACGGCTCACTTGCTTGCTTTCAACTCACACTTGAATCCACTGCTCCTGGTTTTCTGCAAGAGCTACGGGATGATTCAGGAACTGGTAGTTTCTGGGGTTTCAAGTCAAGGGGTAGGACGGTAGGGGCTGTGCAGGACTTGGTTATATCTATGGTTCATGGGAAACCACTTATATTTGTGATTCATACGGATCGGGTGTTAAGAGTATGGGATCTTTCATTTCATGGCAGGATCTTTGCCAATAAGATGAACGGTCCAACAGTGGCAGGAGCTGCATCGGTAAGGTTGTGGGTTGGTCAAGCTGACAATGACTCGAGCATAATTCCTGTGGCAATCTTGTACAGGCATAATTCGGATATTAGCTCAGAGACAATCTACGTATATAGGCTCCATTGTAACGTGAGAGACAGAATTGAATTATTGATATATCGTTCAAAACAAATAATTCATGTTGAGGATGGATGGATTGATGTCAAACTTATTTCCAACAAGATATGCATATTGAAAAATCACGGACTGGTGTTTCTCAAGTTGTTGGATATAAGTGCCGACACGGTAGATGCAGTTTGGGTAGATGCAGTTTGTTATCCTTTGCAAGAGGACTTTGTTGCAGATCAACTCTTTCAAAGTTCAAAACATTCTTCGGATGACCTTCTTTTGATAACTCATTCAATATTTTCATCTTCAAGGGATCATATTCTACCCGTTGTATCTTCTATTTTCTTACGTAGGCTGCTTCTTCCTGGCGTTCATCATAATGCTGCTTTGCGCACAACTTTGTTGGATTATAATAGGCACTGGACAGAGTCAGATTTCCACTCCTTAACTGCTGATGGGTTGAAGAAGGAAATTCTTTCCCTGATTGAACATGAGGCTCTGACTGGTAATTCATCGTCAATATTTTGCTGCTGGGAAATTTTTTGTGCTCGCTATTTCGAAAACTGGTGCAAGAGCAATGCACCATGTGGCCTGCTTGTTGATTCCTCAACCGGCACTGTTGGTTTAATTAGAAAGAGTTCAGTATCTTTTTTTCGTTCTTTGGAGGATATTGAGCGGGTTAATGATGGCTCTTTGGATGAACTTGGCAATATTCCAAGCTTTGGGTTGGATTCATTTGGCAAGGCTGATCGTAAGCTACTTGCTGAAATGCTCAGGTGTGTCACTAATGTCAGCCAGCAATTGGGAAAAACAGCTTCTGCTATATTTTACGAATCACTCATTAGTGCACCACCAGTTATCTCATCTAAAGAAATTATTCCACGCCTATTGAAGATATTAGAAACTGGATATAGTTCAACAGCAGCAGAGCTTCGGATAGCAGATCTTGGAACTGATCTTGCCTGGGAGAAAAATCTAGCAGATCACAAAAATTTGAGGAAATTTTCTATTGACATGATGAAGTCTCTTCATGCTTTGCACGAGAAATCTGGCACATGGAGCCGTATTTTAAGTGTGATTGAGAAGTATCTTAAGTATCTCATACCTCGAAAAATTATACAAAAATTTGATGCTGAAGTAGCATTGGATATTAATGCCTCCATCTTGGTCTCGGCTACGTTTCAAGTTGCAAAGGTTATGTTTGAGTCTGCATTGGATATTCATCTGTTTCTAAGCTATCTAGTGAGCATTAGCAGGCAGATCAATATGCTACATGATGATATATCCAAAATACAACTTGAGTTGGTGCCAATGATTCAAGACGTCATTTATGAGTGGCTTCTCCTGCATTTCTTTGCCACCACACCATCTGAATCAGCTGCAATTGAAGATTTCAGCTCTCAACTTTCATTGTTACAAATTGATAGCAACACTGGTAGAAGATTATGGAATGAGAAGCTTGGGAAGTGCGAGTTCACTCTGGCTTTTATATTCTTACTTAATGTTAGAAGTTCCTCCAAAGACCAGAACCACTTTTCTCCAAGAAGCATACCAAGCGTTGAGGACATAATTGATTCAGTACGAGAGTTTGATAGCTGGATCATGTGGGGCCAGGATGGGGAATCCCTTACTTTCCTGAAGCGTGCAGCGGATCTTGCATTGATCCTGCTAAGGCACGGGCAGTATGATGCTGTTGAGCACTTACTCACTATTGTGGAGGCACATTTAGAAAAGGAGGAGACTTCTCACGGTATTAGAGCTACAGATGGTGGATGGTGCATACTTCATCATCTTTTAGGATGCTGCTTCCTTGCTCAGGCACATCGTGGAGTACATGGGGTTCTGAAAGAGAGGAAAGTTCATGAAGCTATTCGTTGTTTCTTCAGAGCCGCATCTGGAAACGGGTCATCTCATGCTTTACAAAGCTTGCCTCAGGAAGCGTGGCCACATCTTGGTATCGATGGTAGCGTATCAGATGCTGCATGGAGGCTCCATTACTATCAGTGGGCAATGCAGATATGTGAGCATTATAATATTAGTGAAGGTGCATGTCAGTTTGCTCTTGCTGCGCTTGAGCAAGTTGAGGAAGCTTACAGTGCAAATAATGAGTCCCATGACAGAGCTCCATTTAATGAATCAGTATCTACCATCAAAGGAAGACTTTGGGCAAATGTTTGCAAATTTACTTTAGATCTCAATCTCTATTATGATGCATATTGTGCAATAATTTCAAATCCAGATGAGGAGAGCAAGTACATCTGCTTGAGGCGTCTTATAAATGTTCTTTATGAACGTGGTGCCATAAAGATTCTATGTGGTGGTCAGCTACCATTCATTGGTCTTACAGAGAAGGTAGAACAAGAGCTTGCATGGAAGGCAGAGCGGTCAGACATTTTAGCAAAGACAAACTTATATAAGTTGCTTTATGCATTTGAAATGCATCGGCATAACTGGCGACAAGCAGCAAGCTACATGTACCTTTATTCTGCCCGTTTGAGAACTGAAGCATCTCTGAAAGGTTACCAACAATTGTGGCGAGTACTGAAGGAGATACAGAATGGGCTCTCTGCTGCTATCAACGCACTTCATCTTGTTCATCCAGCATATGCTTGGATTGATCCTCTGCTTGCGAGAAGTGCTCTTCACAACGAGCAGTATCCAGGTAGAGAGGCTAGGATAACAATTGAAGACCAGCCAGCAAACAATGATGTTCATCCTCGAAGTTGGAAATCTTTTATAGATATTCAGAAAATTGAGGACGAGTTTGTGCTTACTTCAGCAGAACATCTGCTTTCACTGGCAGATGTCAAATGGACAAATACAGGAACCCAGACAGCACCATTAGAGTTGGTTGATCTTTTGATCCAGACAAATCTATATGATATGGCATTTACAGTTCTTGTAAGATTCTTCAAAGGTTCAGAGTTAAAGAGGGGATTGGAAAGAGTTTTCTCAGCTATGTCACTAAAATGCTTTCCACATATAGTTGATTCTTCAAGGGTTGGGGATGATCCCACAACACAAGGTCTTCTGTTGACTTCTCCAAAGGATGATGTGATCGTTCATGGTTCACCAGATATGTCTTCAGCAAATCAACAATCCAATGGAATAAATCAATGGGGAACTCTCGAGCTTTATCTTGACCGGTATAAAGTGTTCCATGCACGTCTACCACTAATTGTTGCCGAAACTCTTCTTCGTACGGATTCTCAAATCCAACTGCCTCTTTGGTTGGTTAAATTATTCAAGGATGGCAGGAGGGAAAAGACTTTGGGCATGACTAGCCACGAATCAAATCCGGCATCGTTGTTTCAATTATATGTTGATTATGGTCGATATGGAGAAGCTATAAATCTGTTGCTTGAGCACAAAGGGTGTTTTGGATCCATGAGAGCAGCCAACATCATGAATCGTAAAAGACCATTTGGAGTTTGGTTTCCGTATACAATGATTCAGCGCCTCTGGTGCAAACTTGAGGAAATGATAAATTCCGGTCACATGGTAGATCAATGTAAGCAGTTCAAAGATTTACTACACAGAGCTTTGCTGAAACATCTCCAACTGGTAAAGATGGACTCGGAAGAAGCACTTAGTTTTATTTCAGAAAATAAGATGGAAACACTTCGGATGCAATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0008104 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016973 GO:0031090 GO:0031503 GO:0031965 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0034613 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

DUF5311 Nup160

Protein Analysis

1649

Amino Acids

185.89

Weight (kDa)

6.28

Isoelectric Point (pI)

40.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_Nup120_160	PF11715	199 - 662	8.6e-54	Nucleoporin Nup120/160, beta-propeller domain
NUP160_helical_2	PF17238	685 - 872	1.1e-86	NUP160, helical domain
TPR_NUP160_120_M	PF23354	1051 - 1301	1.3e-34	NUP160/120 middle TPR
HEAT_Nup120	PF23300	1052 - 1201	3.3e-12	Nucleoporin Nup120, HEAT repeats
TPR_Nup160_C	PF23347	1353 - 1617	2.4e-10	Nup160 C-terminal TPR

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002601)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33410 AT1G33410
fragaria_vesca	FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400
malus_domestica	MD00G1034800.v1.1 MD05G1063300.v1.1 MD05G1161100.v1.1 MD05G1162300.v1.1 MD05G1162400.v1.1 MD07G1280900.v1.1 MD08G1158600.v1.1 MD10G1009800.v1.1 MD10G1160000.v1.1
prunus_persica	Prupe.5G022000_v2.0.a1
pyrus_communis	pycom10g00600
rosa_chinensis	RchiOBHm_Chr2g0126921 RchiOBHm_Chr4g0402211
rosa_laevigata	RLG00000009106 RLG00000018928
rosa_multiflora	Rmu_sc0000007.1_g000030 Rmu_sc0001405.1_g000051
rosa_roxburghii	Rroxscaffold_1G00047890 Rroxscaffold_2G00116920 Rroxscaffold_5G00346940
rosa_rugosa	Rorug02G0270100 Rorug02G0270200
rosa_samantha	Rh2AG322500 Rh2BG330700 Rh2CG310600 Rh2DG350000 Rh4BG104100 Rh4CG117900 Rh4DG101500
rosa_wichuraiana	Rw2G026160 Rw4G008910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1722, 3342, 3587
AatII	GACGTC	1 cut(s)	2637
Acc16I	TGCGCA	1 cut(s)	1703
AccB1I	GGYRCC	2 cut(s)	2617, 3608
AccB7I	CCANNNNNTGG	5 cut(s)	458, 2033, 2113, 3263, 3647
AccBSI	CCGCTC	3 cut(s)	270, 1987, 3694
AccI	GTMKAC	2 cut(s)	4480, 4754
AccII	CGCG	1 cut(s)	205
AciI	CCGC	9 cut(s)	205, 268, 354, 516, 522, 1987, 2960, 3201, 3694
AcoI	YGGCCR	4 cut(s)	136, 513, 789, 3254
AcsI	RAATTY	8 cut(s)	665, 1782, 1848, 2302, 2312, 2435, 3491, 4798
AcuI	CTGAAG	8 cut(s)	699, 2466, 2969, 3178, 3834, 3875, 4071, 4380
AcyI	GRCGYC	2 cut(s)	2634, 3580
AdeI	CACNNNGTG	4 cut(s)	236, 351, 1898, 3137
AfiI	CCNNNNNNNGG	9 cut(s)	31, 458, 677, 2033, 2113, 2929, 3127, 3263, 3647
AflII	CTTAAG	1 cut(s)	2402
AflIII	ACRYGT	1 cut(s)	3783
AgeI	ACCGGT	1 cut(s)	4455
AhdI	GACNNNNNGTC	2 cut(s)	55, 912
AjiI	CACGTC	1 cut(s)	4478
AjnI	CCWGG	6 cut(s)	30, 979, 1675, 2273, 2922, 3970
AjuI	GAANNNNNNNTTGG	1 cut(s)	28
AleI	CACNNNNGTG	1 cut(s)	3023
Alw21I	GWGCWC	7 cut(s)	651, 1861, 2161, 3012, 3436, 3952, 4680
Alw26I	GTCTC	9 cut(s)	100, 114, 403, 576, 1295, 1332, 2340, 2485, 3044
Alw44I	GTGCAC	1 cut(s)	2157
AlwNI	CAGNNNCTG	4 cut(s)	1016, 1205, 2129, 3290
Ama87I	CYCGRG	3 cut(s)	644, 1246, 4439
AoxI	GGCC	7 cut(s)	77, 136, 513, 789, 1900, 2920, 3254
ApaLI	GTGCAC	1 cut(s)	2157
ApoI	RAATTY	8 cut(s)	665, 1782, 1848, 2302, 2312, 2435, 3491, 4798
ArsI	GACNNNNNNTTYG	2 cut(s)	3404, 3436
AseI	ATTAAT	1 cut(s)	2463
AsiGI	ACCGGT	1 cut(s)	4455
Asp700I	GAANNNNTTC	5 cut(s)	1948, 2187, 3180, 4503, 4932
AspLEI	GCGC	4 cut(s)	563, 1704, 3382, 4770
AspS9I	GGNCC	4 cut(s)	819, 878, 1187, 2920
AsuC2I	CCSGG	1 cut(s)	754
AsuHPI	GGTGA	1 cut(s)	4374
AsuII	TTCGAA	1 cut(s)	1869
AvaI	CYCGRG	3 cut(s)	644, 1246, 4439
AvaII	GGWCC	3 cut(s)	819, 878, 1187
AxyI	CCTNAGG	2 cut(s)	282, 3243
BaeGI	GKGCMC	2 cut(s)	544, 2161
BalI	TGGCCA	2 cut(s)	138, 3256
BamHI	GGATCC	1 cut(s)	4694
BanI	GGYRCC	2 cut(s)	2617, 3608
BanII	GRGCYC	3 cut(s)	651, 3436, 3876
BauI	CACGAG	1 cut(s)	2351
BbsI	GAAGAC	4 cut(s)	3475, 4005, 4331, 4386
Bbv12I	GWGCWC	7 cut(s)	651, 1861, 2161, 3012, 3436, 3952, 4680
BceAI	ACGGC	2 cut(s)	952, 2358
BcgI	CGANNNNNNTGC	2 cut(s)	635, 669
BciT130I	CCWGG	6 cut(s)	32, 981, 1677, 2275, 2924, 3972
BciVI	GTATCC	1 cut(s)	3978
BcnI	CCSGG	1 cut(s)	754
BcoDI	GTCTC	9 cut(s)	100, 114, 403, 576, 1295, 1332, 2340, 2485, 3044
BfaI	CTAG	5 cut(s)	828, 2288, 2552, 3984, 4593
BfmI	CTRYAG	1 cut(s)	3072
BfoI	RGCGCY	1 cut(s)	4771
BfrI	CTTAAG	1 cut(s)	2402
BfuI	GTATCC	1 cut(s)	3978
BglII	AGATCT	2 cut(s)	2254, 3502
BmcAI	AGTACT	1 cut(s)	3852
Bme1390I	CCNGG	7 cut(s)	32, 754, 981, 1677, 2275, 2924, 3972
Bme18I	GGWCC	3 cut(s)	819, 878, 1187
BmeRI	GACNNNNNGTC	2 cut(s)	55, 912
BmeT110I	CYCGRG	3 cut(s)	644, 1246, 4439
BmgBI	CACGTC	1 cut(s)	4478
BmgT120I	GGNCC	4 cut(s)	819, 878, 1187, 2920
BmrFI	CCNGG	7 cut(s)	32, 754, 981, 1677, 2275, 2924, 3972
BpiI	GAAGAC	4 cut(s)	3475, 4005, 4331, 4386
BplI	GAGNNNNNCTC	4 cut(s)	35, 67, 3934, 3966
BpmI	CTGGAG	1 cut(s)	256
Bpu10I	CCTNAGC	3 cut(s)	2084, 2981, 3126
Bpu14I	TTCGAA	1 cut(s)	1869
BpuEI	CTTGAG	6 cut(s)	1445, 2630, 3404, 3595, 4694, 4805
BpuMI	CCSGG	1 cut(s)	754
Bsa29I	ATCGAT	1 cut(s)	3270
BsaAI	YACGTR	2 cut(s)	1312, 1661
BsaBI	GATNNNNATC	4 cut(s)	743, 2532, 3507, 4023
BsaHI	GRCGYC	2 cut(s)	2634, 3580
BsaI	GGTCTC	4 cut(s)	100, 403, 576, 2485
BsaJI	CCNNGG	3 cut(s)	457, 786, 2274
BsaWI	WCCGGW	3 cut(s)	908, 4455, 4802
BsaXI	ACNNNNNCTCC	6 cut(s)	254, 279, 284, 309, 3548, 3578
Bsc4I	CCNNNNNNNGG	9 cut(s)	31, 458, 677, 2033, 2113, 2929, 3127, 3263, 3647
Bse118I	RCCGGY	2 cut(s)	1922, 4455
Bse21I	CCTNAGG	2 cut(s)	282, 3243
Bse3DI	GCAATG	2 cut(s)	1894, 3327
Bse8I	GATNNNNATC	4 cut(s)	743, 2532, 3507, 4023
BseBI	CCWGG	6 cut(s)	32, 981, 1677, 2275, 2924, 3972
BseCI	ATCGAT	1 cut(s)	3270
BseDI	CCNNGG	3 cut(s)	457, 786, 2274
BseJI	GATNNNNATC	4 cut(s)	743, 2532, 3507, 4023
BseLI	CCNNNNNNNGG	9 cut(s)	31, 458, 677, 2033, 2113, 2929, 3127, 3263, 3647
BseMI	GCAATG	2 cut(s)	1894, 3327
BseMII	CTCAG	7 cut(s)	104, 273, 1311, 2098, 3140, 3257, 4278
BseRI	GAGGAG	2 cut(s)	3062, 3569
BseSI	GKGCMC	2 cut(s)	544, 2161
BseYI	CCCAGC	1 cut(s)	1842
BsgI	GTGCAG	2 cut(s)	1085, 2976
Bsh1236I	CGCG	1 cut(s)	205
Bsh1285I	CGRYCG	1 cut(s)	909
BshFI	GGCC	7 cut(s)	79, 138, 515, 791, 1902, 2922, 3256
BshNI	GGYRCC	2 cut(s)	2617, 3608
BshTI	ACCGGT	1 cut(s)	4455
BshVI	ATCGAT	1 cut(s)	3270
BsiEI	CGRYCG	1 cut(s)	909
BsiHKAI	GWGCWC	7 cut(s)	651, 1861, 2161, 3012, 3436, 3952, 4680
BsiHKCI	CYCGRG	3 cut(s)	644, 1246, 4439
BsiSI	CCGG	7 cut(s)	753, 792, 909, 1923, 4456, 4610, 4803
BsiWI	CGTACG	1 cut(s)	4511
BslFI	GGGAC	2 cut(s)	832, 3406
BslI	CCNNNNNNNGG	9 cut(s)	31, 458, 677, 2033, 2113, 2929, 3127, 3263, 3647
BsmAI	GTCTC	9 cut(s)	100, 114, 403, 576, 1295, 1332, 2340, 2485, 3044
BsmFI	GGGAC	2 cut(s)	832, 3406
BsnI	GGCC	7 cut(s)	79, 138, 515, 791, 1902, 2922, 3256
Bso31I	GGTCTC	4 cut(s)	100, 403, 576, 2485
BsoBI	CYCGRG	3 cut(s)	644, 1246, 4439
Bsp119I	TTCGAA	1 cut(s)	1869
Bsp1286I	GDGCHC	9 cut(s)	544, 651, 1861, 2161, 3012, 3436, 3876, 3952, 4680
Bsp1407I	TGTACA	1 cut(s)	1272
BspACI	CCGC	9 cut(s)	205, 268, 354, 516, 522, 1987, 2960, 3201, 3694
BspANI	GGCC	7 cut(s)	79, 138, 515, 791, 1902, 2922, 3256
BspCNI	CTCAG	7 cut(s)	105, 274, 1310, 2097, 3139, 3256, 4277
BspDI	ATCGAT	1 cut(s)	3270
BspFNI	CGCG	1 cut(s)	205
BspHI	TCATGA	2 cut(s)	3172, 4716
BspQI	GCTCTTC	1 cut(s)	3957
BspT104I	TTCGAA	1 cut(s)	1869
BspT107I	GGYRCC	2 cut(s)	2617, 3608
BspTI	CTTAAG	1 cut(s)	2402
BspTNI	GGTCTC	4 cut(s)	100, 403, 576, 2485
BsrBI	CCGCTC	3 cut(s)	270, 1987, 3694
BsrDI	GCAATG	2 cut(s)	1894, 3327
BsrFI	RCCGGY	2 cut(s)	1922, 4455
BsrGI	TGTACA	1 cut(s)	1272
BssAI	RCCGGY	2 cut(s)	1922, 4455
BssECI	CCNNGG	3 cut(s)	457, 786, 2274
BssNAI	GTATAC	1 cut(s)	4755
BssNI	GRCGYC	2 cut(s)	2634, 3580
BssSI	CACGAG	1 cut(s)	2351
BssT1I	CCWWGG	1 cut(s)	457
Bst1107I	GTATAC	1 cut(s)	4755
Bst2BI	CACGAG	1 cut(s)	2351
Bst2UI	CCWGG	6 cut(s)	32, 981, 1677, 2275, 2924, 3972
Bst4CI	ACNGT	9 cut(s)	787, 1054, 1187, 1195, 1489, 1930, 3062, 3407, 4207
Bst6I	CTCTTC	5 cut(s)	379, 715, 2343, 3957, 4509
BstACI	GRCGYC	2 cut(s)	2634, 3580
BstAFI	CTTAAG	1 cut(s)	2402
BstAPI	GCANNNNNTGC	2 cut(s)	1205, 3530
BstAUI	TGTACA	1 cut(s)	1272
BstBAI	YACGTR	2 cut(s)	1312, 1661
BstBI	TTCGAA	1 cut(s)	1869
BstDSI	CCRYGG	1 cut(s)	786
BstEII	GGTNACC	1 cut(s)	3830
BstENI	CCTNNNNNAGG	1 cut(s)	3125
BstFNI	CGCG	1 cut(s)	205
BstH2I	RGCGCY	1 cut(s)	4771
BstHHI	GCGC	4 cut(s)	563, 1704, 3382, 4770
BstMAI	GTCTC	9 cut(s)	100, 114, 403, 576, 1295, 1332, 2340, 2485, 3044
BstMCI	CGRYCG	1 cut(s)	909
BstNI	CCWGG	6 cut(s)	32, 981, 1677, 2275, 2924, 3972
BstNSI	RCATGY	3 cut(s)	89, 3363, 3787
BstPI	GGTNACC	1 cut(s)	3830
BstSCI	CCNGG	7 cut(s)	30, 752, 979, 1675, 2273, 2922, 3970
BstSFI	CTRYAG	1 cut(s)	3072
BstSLI	GKGCMC	2 cut(s)	544, 2161
BstSNI	TACGTA	2 cut(s)	1312, 1661
BstUI	CGCG	1 cut(s)	205
BstV2I	GAAGAC	4 cut(s)	3475, 4005, 4331, 4386
BstX2I	RGATCY	7 cut(s)	249, 1144, 1164, 2254, 2962, 3502, 4694
BstXI	CCANNNNNNTGG	1 cut(s)	418
BstYI	RGATCY	7 cut(s)	249, 1144, 1164, 2254, 2962, 3502, 4694
BstZ17I	GTATAC	1 cut(s)	4755
Bsu15I	ATCGAT	1 cut(s)	3270
Bsu36I	CCTNAGG	2 cut(s)	282, 3243
BsuI	GTATCC	1 cut(s)	3978
BsuRI	GGCC	7 cut(s)	79, 138, 515, 791, 1902, 2922, 3256
BsuTUI	ATCGAT	1 cut(s)	3270
BtgI	CCRYGG	1 cut(s)	786
BtgZI	GCGATG	2 cut(s)	186, 556
BtrI	CACGTC	1 cut(s)	4478
BtsI	GCAGTG	2 cut(s)	895, 972
CaiI	CAGNNNCTG	4 cut(s)	1016, 1205, 2129, 3290
CciI	TCATGA	2 cut(s)	3172, 4716
CfoI	GCGC	4 cut(s)	563, 1704, 3382, 4770
Cfr10I	RCCGGY	2 cut(s)	1922, 4455
Cfr13I	GGNCC	4 cut(s)	819, 878, 1187, 2920
ClaI	ATCGAT	1 cut(s)	3270
CseI	GACGC	1 cut(s)	3569
CsiI	ACCWGGT	1 cut(s)	30
CspAI	ACCGGT	1 cut(s)	4455
CspCI	CAANNNNNGTGG	4 cut(s)	2181, 2216, 3824, 3859
DraIII	CACNNNGTG	4 cut(s)	236, 351, 1898, 3137
DriI	GACNNNNNGTC	2 cut(s)	55, 912
EaeI	YGGCCR	4 cut(s)	136, 513, 789, 3254
Eam1104I	CTCTTC	5 cut(s)	379, 715, 2343, 3957, 4509
Eam1105I	GACNNNNNGTC	2 cut(s)	55, 912
EarI	CTCTTC	5 cut(s)	379, 715, 2343, 3957, 4509
Ecl136II	GAGCTC	2 cut(s)	649, 3434
Eco105I	TACGTA	2 cut(s)	1312, 1661
Eco130I	CCWWGG	1 cut(s)	457
Eco24I	GRGCYC	3 cut(s)	651, 3436, 3876
Eco31I	GGTCTC	4 cut(s)	100, 403, 576, 2485
Eco32I	GATATC	1 cut(s)	745
Eco47I	GGWCC	3 cut(s)	819, 878, 1187
Eco53kI	GAGCTC	2 cut(s)	649, 3434
Eco57I	CTGAAG	8 cut(s)	699, 2466, 2969, 3178, 3834, 3875, 4071, 4380
Eco81I	CCTNAGG	2 cut(s)	282, 3243
Eco88I	CYCGRG	3 cut(s)	644, 1246, 4439
Eco91I	GGTNACC	1 cut(s)	3830
EcoICRI	GAGCTC	2 cut(s)	649, 3434
EcoNI	CCTNNNNNAGG	1 cut(s)	3125
EcoO65I	GGTNACC	1 cut(s)	3830
EcoRII	CCWGG	6 cut(s)	30, 979, 1675, 2273, 2922, 3970
EcoRV	GATATC	1 cut(s)	745
EcoT14I	CCWWGG	1 cut(s)	457
EcoT22I	ATGCAT	6 cut(s)	91, 781, 1433, 3526, 3742, 3753
EcoT38I	GRGCYC	3 cut(s)	651, 3436, 3876
ErhI	CCWWGG	1 cut(s)	457
FalI	AAGNNNNNCTT	6 cut(s)	3456, 3488, 3704, 3736, 4884, 4916
FaqI	GGGAC	2 cut(s)	832, 3406
FauI	CCCGC	2 cut(s)	275, 1980
FauNDI	CATATG	2 cut(s)	602, 3916
FblI	GTMKAC	2 cut(s)	4480, 4754
FriOI	GRGCYC	3 cut(s)	651, 3436, 3876
FspBI	CTAG	5 cut(s)	828, 2288, 2552, 3984, 4593
FspI	TGCGCA	1 cut(s)	1703
GlaI	GCGC	4 cut(s)	562, 1703, 3381, 4769
GsaI	CCCAGC	1 cut(s)	1846
GsuI	CTGGAG	1 cut(s)	256
HaeII	RGCGCY	1 cut(s)	4771
HaeIII	GGCC	7 cut(s)	79, 138, 515, 791, 1902, 2922, 3256
HapII	CCGG	7 cut(s)	753, 792, 909, 1923, 4456, 4610, 4803
HgaI	GACGC	1 cut(s)	3569
HhaI	GCGC	4 cut(s)	563, 1704, 3382, 4770
Hin1I	GRCGYC	2 cut(s)	2634, 3580
Hin6I	GCGC	4 cut(s)	561, 1702, 3380, 4768
HinP1I	GCGC	4 cut(s)	561, 1702, 3380, 4768
HincII	GTYRAC	1 cut(s)	4347
HindII	GTYRAC	1 cut(s)	4347
HindIII	AAGCTT	5 cut(s)	124, 2029, 2768, 3235, 3399
HpaII	CCGG	7 cut(s)	753, 792, 909, 1923, 4456, 4610, 4803
HphI	GGTGA	1 cut(s)	4374
Hpy166II	GTNNAC	8 cut(s)	168, 243, 2159, 2787, 4347, 4382, 4481, 4755
Hpy8I	GTNNAC	8 cut(s)	168, 243, 2159, 2787, 4347, 4382, 4481, 4755
HpyAV	CCTTC	6 cut(s)	1595, 1771, 3347, 3655, 3679, 3850
HpyCH4III	ACNGT	9 cut(s)	787, 1054, 1187, 1195, 1489, 1930, 3062, 3407, 4207
HpyCH4IV	ACGT	7 cut(s)	1311, 1332, 1660, 2489, 2634, 3604, 4477
HpySE526I	ACGT	7 cut(s)	1311, 1332, 1660, 2489, 2634, 3604, 4477
Hsp92I	GRCGYC	2 cut(s)	2634, 3580
HspAI	GCGC	4 cut(s)	561, 1702, 3380, 4768
LguI	GCTCTTC	1 cut(s)	3957
MabI	ACCWGGT	1 cut(s)	30
MaeI	CTAG	5 cut(s)	828, 2288, 2552, 3984, 4593
MaeII	ACGT	7 cut(s)	1311, 1332, 1660, 2489, 2634, 3604, 4477
MaeIII	GTNAC	6 cut(s)	1328, 2092, 2724, 3830, 4272, 4805
MbiI	CCGCTC	3 cut(s)	270, 1987, 3694
MfeI	CAATTG	4 cut(s)	2111, 2694, 3839, 3993
MflI	RGATCY	7 cut(s)	249, 1144, 1164, 2254, 2962, 3502, 4694
MhlI	GDGCHC	9 cut(s)	544, 651, 1861, 2161, 3012, 3436, 3876, 3952, 4680
MlsI	TGGCCA	2 cut(s)	138, 3256
MluNI	TGGCCA	2 cut(s)	138, 3256
MlyI	GAGTC	7 cut(s)	44, 804, 1238, 1748, 2525, 3428, 4880
Mox20I	TGGCCA	2 cut(s)	138, 3256
Mph1103I	ATGCAT	6 cut(s)	91, 781, 1433, 3526, 3742, 3753
MroXI	GAANNNNTTC	5 cut(s)	1948, 2187, 3180, 4503, 4932
MscI	TGGCCA	2 cut(s)	138, 3256
MslI	CAYNNNNRTG	5 cut(s)	88, 446, 1689, 3023, 4279
Msp20I	TGGCCA	2 cut(s)	138, 3256
MspA1I	CMGCKG	3 cut(s)	276, 2690, 2960
MspCI	CTTAAG	1 cut(s)	2402
MspI	CCGG	7 cut(s)	753, 792, 909, 1923, 4456, 4610, 4803
MspR9I	CCNGG	7 cut(s)	32, 754, 981, 1677, 2275, 2924, 3972
MunI	CAATTG	4 cut(s)	2111, 2694, 3839, 3993
MvaI	CCWGG	6 cut(s)	32, 981, 1677, 2275, 2924, 3972
MvnI	CGCG	1 cut(s)	205
NciI	CCSGG	1 cut(s)	754
NdeI	CATATG	2 cut(s)	602, 3916
NmeAIII	GCCGAG	1 cut(s)	2462
NmuCI	GTSAC	3 cut(s)	2092, 4272, 4805
NsbI	TGCGCA	1 cut(s)	1703
NsiI	ATGCAT	6 cut(s)	91, 781, 1433, 3526, 3742, 3753
NspI	RCATGY	3 cut(s)	89, 3363, 3787
NspV	TTCGAA	1 cut(s)	1869
OliI	CACNNNNGTG	1 cut(s)	3023
PaeR7I	CTCGAG	3 cut(s)	644, 1246, 4439
PagI	TCATGA	2 cut(s)	3172, 4716
PciI	ACATGT	1 cut(s)	3783
PciSI	GCTCTTC	1 cut(s)	3957
PcsI	WCGNNNNNNNCGW	2 cut(s)	642, 3276
PdmI	GAANNNNTTC	5 cut(s)	1948, 2187, 3180, 4503, 4932
Pfl23II	CGTACG	1 cut(s)	4511
PflFI	GACNNNGTC	1 cut(s)	1738
PflMI	CCANNNNNTGG	5 cut(s)	458, 2033, 2113, 3263, 3647
PfoI	TCCNGGA	1 cut(s)	752
PinAI	ACCGGT	1 cut(s)	4455
PleI	GAGTC	7 cut(s)	44, 803, 1238, 1747, 2524, 3427, 4880
PpsI	GAGTC	7 cut(s)	44, 803, 1238, 1747, 2524, 3427, 4880
Ppu21I	YACGTR	2 cut(s)	1312, 1661
PscI	ACATGT	1 cut(s)	3783
PshBI	ATTAAT	1 cut(s)	2463
PsiI	TTATAA	3 cut(s)	1722, 3342, 3587
Psp124BI	GAGCTC	2 cut(s)	651, 3436
Psp6I	CCWGG	6 cut(s)	30, 979, 1675, 2273, 2922, 3970
PspEI	GGTNACC	1 cut(s)	3830
PspFI	CCCAGC	1 cut(s)	1842
PspGI	CCWGG	6 cut(s)	30, 979, 1675, 2273, 2922, 3970
PspLI	CGTACG	1 cut(s)	4511
PspPI	GGNCC	4 cut(s)	819, 878, 1187, 2920
PspXI	VCTCGAGB	2 cut(s)	644, 1246
PsrI	GAACNNNNNNTAC	4 cut(s)	3135, 3167, 4451, 4483
PstNI	CAGNNNCTG	4 cut(s)	1016, 1205, 2129, 3290
PsuI	RGATCY	7 cut(s)	249, 1144, 1164, 2254, 2962, 3502, 4694
PsyI	GACNNNGTC	1 cut(s)	1738
PvuII	CAGCTG	2 cut(s)	276, 2690
RseI	CAYNNNNRTG	5 cut(s)	88, 446, 1689, 3023, 4279
SacI	GAGCTC	2 cut(s)	651, 3436
SapI	GCTCTTC	1 cut(s)	3957
Sau96I	GGNCC	4 cut(s)	819, 878, 1187, 2920
ScaI	AGTACT	1 cut(s)	3852
SchI	GAGTC	7 cut(s)	44, 804, 1238, 1748, 2525, 3428, 4880
ScrFI	CCNGG	7 cut(s)	32, 754, 981, 1677, 2275, 2924, 3972
SduI	GDGCHC	9 cut(s)	544, 651, 1861, 2161, 3012, 3436, 3876, 3952, 4680
SexAI	ACCWGGT	1 cut(s)	30
SfcI	CTRYAG	1 cut(s)	3072
Sfr274I	CTCGAG	3 cut(s)	644, 1246, 4439
SfuI	TTCGAA	1 cut(s)	1869
SinI	GGWCC	3 cut(s)	819, 878, 1187
SlaI	CTCGAG	3 cut(s)	644, 1246, 4439
SmiMI	CAYNNNNRTG	5 cut(s)	88, 446, 1689, 3023, 4279
SnaBI	TACGTA	2 cut(s)	1312, 1661
SsiI	CCGC	9 cut(s)	205, 268, 354, 516, 522, 1987, 2960, 3201, 3694
SspI	AATATT	4 cut(s)	1608, 1833, 2023, 3346
SspMI	CTAG	5 cut(s)	828, 2288, 2552, 3984, 4593
SstI	GAGCTC	2 cut(s)	651, 3436
StyD4I	CCNGG	7 cut(s)	30, 752, 979, 1675, 2273, 2922, 3970
StyI	CCWWGG	1 cut(s)	457
TaaI	ACNGT	9 cut(s)	787, 1054, 1187, 1195, 1489, 1930, 3062, 3407, 4207
TaiI	ACGT	7 cut(s)	1314, 1335, 1663, 2492, 2637, 3607, 4480
TatI	WGTACW	4 cut(s)	1272, 3142, 3564, 3850
TauI	GCSGC	2 cut(s)	518, 3203
TseFI	GTSAC	3 cut(s)	2092, 4272, 4805
Tsp45I	GTSAC	3 cut(s)	2092, 4272, 4805
TspGWI	ACGGA	7 cut(s)	117, 260, 891, 1136, 1461, 4529, 4740
Tth111I	GACNNNGTC	1 cut(s)	1738
Van91I	CCANNNNNTGG	5 cut(s)	458, 2033, 2113, 3263, 3647
Vha464I	CTTAAG	1 cut(s)	2402
VneI	GTGCAC	1 cut(s)	2157
VpaK11BI	GGWCC	3 cut(s)	819, 878, 1187
VspI	ATTAAT	1 cut(s)	2463
XagI	CCTNNNNNAGG	1 cut(s)	3125
XapI	RAATTY	8 cut(s)	665, 1782, 1848, 2302, 2312, 2435, 3491, 4798
XceI	RCATGY	3 cut(s)	89, 3363, 3787
XcmI	CCANNNNNNNNNTGG	3 cut(s)	87, 3918, 4740
XhoI	CTCGAG	3 cut(s)	644, 1246, 4439
XmiI	GTMKAC	2 cut(s)	4480, 4754
XmnI	GAANNNNTTC	5 cut(s)	1948, 2187, 3180, 4503, 4932
XspI	CTAG	5 cut(s)	828, 2288, 2552, 3984, 4593
ZraI	GACGTC	1 cut(s)	2635
ZrmI	AGTACT	1 cut(s)	3852
Zsp2I	ATGCAT	6 cut(s)	91, 781, 1433, 3526, 3742, 3753

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.