Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002601

Physical Location & Seq

Genomic Coordinates Reverse (-)

42924004 .. 42944859

Gene Structure 20856 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018928

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4578 bp

ATGCGTTTTGTTTGGAGACGAATACAGGCTGACTTTTTGTTCTGTGTATTTGTGAAGGATGTGTGGAAAGAATACACAGGTTGGCCTTTAAATAATATGGAGAGGTCTTACAAGTTCATGGTGAAACATGTTCAGCTATGGAAGGTTGCATTTCACAGTACTTCTCCTAGATGGATCCACAGTGTCTATCTTGCTGCCATTGCAGCCTACTATGCCAAGTTTCATCCGGGGGTCAATAGGTGTTACTGCCCATCGGAGGAGGTAGCTCTAGTATATGGCCTTGAAGAGTCCCAAATACGTGTTTTTGGCTTGCCCATCAGGCCCTCTTTTGCGCGAGCGGTTATCTCCAAGGTTAGAGGATTTGAGACACAAATGGAGAAATGGATGGGATCTTGTGATTGCATCATAACAAAAATTGATGTCGGTTCCAGGAGCCACCCCTATTTGCTCTATGCATTGACCGTCGCCGGAGTCGCTTACCTTTTGAGACTTGGAACCGTCTCTAGTTATGCTTCCAACTCTGTTATTAGAGAGTTCAGCTTGCATCCCCACGGACCTATAACCAGTGCTGCAGCAACGCCAACCGGATGTCTTGTTGTAGGTCGGAATGACGGCTCACTTGCTTGCTTTCAACTCACACTTGAATCCACTGCTCCTGGTTTTCTGCAAGAGCTACGGGATGATCCAGGAATCGGTAGTTTGTGGGGTTTCATGTCAAGGGGTAGGACGGTAGGCGCTGTGCAGGACTTGACTCTATCTATGGTTCATGGGAAACCACTTATATTCGTTATTCATACGGATGGGGTGTTACGAGTATGGGATATTTCATTTCATAGCAGGCTCTTTTCCCATAAGATGAACGGTCTAACAATGGCAGGAGCTGCATTGGTAAGGTTGTGGGTTGGTCAAGCTGACAATGATTCAAGCATAATTCCTTTGGCAATCTTGTACAGACATAATTCGGAAATGAGCTCAGAGACGATCTACGTATATAGCCTCCATTGTAACATGGGAGACAGAATTGTTTTATCGTTGGATCCTTCAGAACAAATTATTCCTGTTGAGGAGGATGGATGGATTGATGTCAAACTTATTTCCAACAAGATATGCATATTGAAAAATCAGGGACTAGTGCTTCACAAGTTGTTGCATACAAATGTCAACATGGTAGATGCAGTTTGTTATGCTTTGCAAGAGGACTTCGTTGCAGATCAACTCTTTCAAAGTTCTGAACATTCTTCGGATGACCTTCTTTTGATAACTCATTCAATATTTTCATCTTCAAAGGATCATATTCTACCCATTGTATCTTCTATTTTCTTACGTAGGCTGCTTCTTCCTGGCATTCATCATAATGCTGCTTTGCGCACAACTTTGTTGGATTATAATAGGCACTGGACTGAGTCAGATTTCCACTCCTTAACTGCTGACGGGTTGAAGAAGGAAATTCTTTCCCTGATTGAACATGAGGGTCTGACTGGTAATTCATCGTCAATATTTTGCTGCTGGAAAATTTTTTGTGCTCGCTATTTCCAAAACTGGTGCAAGAGCAATGCACCATGTGGTCTGCTTGTTGATTCCTCAACCGGCACTGTTGGTTTAATTAGAAAGAGTTCAGTATCTCTTTTTCGTTCTTTGGAGGATATTGAGCGGGTTAATGATGGCTCTTTGGATGAACTTGGCAATTTTCCAAGCTTTGGGTTGGATTCATTTGATGAGGCTCTTGATTGTGAGCTGCTTGCTGAAATGCTCAGATGTGTCATTAACATCAGCCAGCAATTGGGCAAAACAGCTTCTGCTATATTTTATGAATCACTCATTAGTGCACCACCAGTTATCTCATCTGAAGAAATTATTCCGCGCCTATTGAAGATATTAGAAACTGGATATAGTTCAACAGCCGCAGTGCTTCATATATCAGATATTGGACCTGATGTTGCCTGGGAGAAAAATCTAGCAGATCACAAAAATTTGAGGAAATTTTCAATTGACATGATGTTGTCTCTTCATGCTTTGCACGAGAAATCTGGCACATGGAGCCGTATTTTGAGTGTGATTGAGAACTATCTAAAGTATCTCTTACCTCGGAAAATTATACAAAAATTTGATGCTGAAGTAGCATTGGATATCAATGCCTCCATCTTGGTCCAGGCTATGTCTCAAGTTGCAAAGGTTATGTTTGAGTCTGCACTGGATATTCATCTGTTTCTAAGCTATCTAGTGAGCATTAGTGGGCAGATCAATATGCTACATGATGATATATCCAAAATACAGCTTGAGTTGGTTCCAATGATTCAAGAAATCATTTATGAGTGGCTTCTCTTACATTTCTTTGCCACCACACCATCTGAATCAGCTGCAATTGAAGATTTCAGCTCTCAACTTTCATTGTTACAAATTGATAGCAACACAGGCAGAAGATCATGGAATGAGAAGCTTGGGAAGTGCGAGGTCACTCTGGCTTTTATATTCTTACTTAATGTTAGAAGTTCCTCCAAAGACCAGAGCCACTTTTCTCCAAGAAGCATACCAAACGTTCAGGACATAATTGATTCAATACGAGAATTTGCAAGCTGGATCATGTGGGGCCAGAATGGGGAATCCCTTACTGTCCTGAGGCGTGCAGCGGATCTTGCATTGATCCTGCTAAAGCACGGGCAGTATGATGCTGTTGAGCACCTACTCACTATTGTGGAGGCACATTTACAAAAGGAGAAGACTTCTCATCGTATTCAAGCTACAGATGGCGGATGGTGCGTACTGCATCATCTTTTAGGATGCTGTTTCCTTGCTCAAGCACATCGTGGATTACATGGGGTCCTGAAAGAGAGGAAAGTTCATGAAGCTGTTCGCTGTTTCTTCAGAGCTGCATCTGGAAACGGCTCAGCGCAGGCTTTACAAAGCTTGCCTCAGGAAGCGGGGTTGCCACATCTTGGTATTGATGGTAGCGTATCAGATGCTGCATGGAGGCTCCATTACTATCAGTGGGCAATGCAGATATTTGAGCAATATAATATTAGTGAAGGTGCATGTCAGTTTGCTCTTGCTGCGCTTGAGCAAGTTGAGGAAACTTATAGTGCAAACAATGAGTCCCATGACAGAGCTCCATTTGATGAATCAGTATCTACCATCAAAGGAAGACTTTGGGCACATGTTTGCAAGTTTACTTTAGATCTCAATCTCTTTTATGATGCGTATTGTGCAATAATTTCAAATCCAGATGAGGAGAGCAAGCACATCTGGTTGAGGCGTCTTATAATTGTTCTTTATGAACGTGGTGCCATAAAGATTCTTTGTGGTGGTCAGCTACCCTTCATTGGTCTTACAGAGAAGGTGGAACAAGAGCTTGCATGGAAGGCTGAACGGTCAGACATTTTAGCAAAGCCTAACTTATATAAGTTGCTTTATGCATTTGAAATGCATCGGCATAACTGGAGAAAAGCAGCAAGCTACATGTACCTTTATTCTGCTCGTTTGAGAACTGAAACATCTCTGAAAGGGTACCAGCAATTGTGGCGAGCACTGAAGGAGATACTGAATGGGCTCTCTGCTGCTATCAACGCACTTCATCTTGTTCATCCAGCATATGCTTGGATTGATCCTCTCCTTGAGAGAAATGCTATTCACAATGAGCAGTATCCAAGTAAAAAGGCTAGAATAACAATTGAAGAACAGCCAGCAAGCAATGATGTTGATCCTCAAAGTTGGAAATCTTATATAGATATTCAGAAAATTGAAAAAGAGTTTGTGCTTGCTTCAGCAGAATATTTGCTTTCACTGGCACATGTCAAATGGACAAATACAGGAACCCAGAAAGCACCATTGGAGTTGGTTGATCTCTTGATCCAGACAAATCTATATGATATGGCATTTACAGTTCTCTTAAGATTCTTCAAAGGTTCAGAGTTAAAGAGGGGATTGGAAAGAGTTTTCTCTGCTATGTCACTAAAATGCTGTCCACATATAGTTAATTCTTCAAGGGTCGGGGATGATTCCACAACACAGGGTCTCCTATTGACTTCTTCAAAGGATGAAGTGATCGTTCATGGTTCACTAGATGCAAGTTCAGTAAATCAACAATCCAGCGGAATTAATCAATGGGGAACTCTTGAGCTTTATCTTGACCGATATAAAGTCTTCCATGCACGTCTACCATTTATTGTTGCTGAAACTCTTCTTCGTACGGATTCTCAAATCGAACTGCCTCTTTGGTTGGTTAAATTATTCAAGGATGGCAGGAGGGAAAAGACATTGGGGATGACTGGCCAAGAATCAAGTCCGGCATTATTATTTCAACTATATGTTGATTATGGTCGATATAGAGAAGCTACAAATCTGTTGCTTGAGTACATAGGTTCTTTAGCATCCACGAGACCAGCCAACATCATGAATCGTAAAAGACCATTTGGAGTTTGGTTTCCATATACAACAATTCAGCGCCTCTGGTGCCAACTTGAGGAAATGATAAATTCAGGTCACATGGTAGATCAATGTAAGCAGCTCAAAGATTTGCTACATGGAGCTTTGCTGAAACATCTCCACCTGGTAAAAGCGGACTCGGAAGATGCACTTAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0008104 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016973 GO:0031090 GO:0031503 GO:0031965 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0034613 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

DUF5311 Nup160

Protein Analysis

1526

Amino Acids

172.35

Weight (kDa)

6.37

Isoelectric Point (pI)

42.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MGDG_synth	PF06925	16 - 73	3.1e-10	Monogalactosyldiacylglycerol (MGDG) synthase
Beta-prop_Nup120_160	PF11715	145 - 550	4e-48	Nucleoporin Nup120/160, beta-propeller domain
NUP160_helical_2	PF17238	567 - 761	2.8e-91	NUP160, helical domain
TPR_NUP160_120_M	PF23354	940 - 1191	4.9e-35	NUP160/120 middle TPR
HEAT_Nup120	PF23300	941 - 1090	6.5e-13	Nucleoporin Nup120, HEAT repeats
TPR_Nup160_C	PF23347	1242 - 1506	2e-13	Nup160 C-terminal TPR

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002601)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33410 AT1G33410
fragaria_vesca	FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400
malus_domestica	MD00G1034800.v1.1 MD05G1063300.v1.1 MD05G1161100.v1.1 MD05G1162300.v1.1 MD05G1162400.v1.1 MD07G1280900.v1.1 MD08G1158600.v1.1 MD10G1009800.v1.1 MD10G1160000.v1.1
prunus_persica	Prupe.5G022000_v2.0.a1
pyrus_communis	pycom10g00600
rosa_chinensis	RchiOBHm_Chr2g0126921 RchiOBHm_Chr4g0402211
rosa_laevigata	RLG00000009106 RLG00000018928
rosa_multiflora	Rmu_sc0000007.1_g000030 Rmu_sc0001405.1_g000051
rosa_roxburghii	Rroxscaffold_1G00047890 Rroxscaffold_2G00116920 Rroxscaffold_5G00346940
rosa_rugosa	Rorug02G0270100 Rorug02G0270200
rosa_samantha	Rh2AG322500 Rh2BG330700 Rh2CG310600 Rh2DG350000 Rh4BG104100 Rh4CG117900 Rh4DG101500
rosa_wichuraiana	Rw2G026160 Rw4G008910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1386, 3257
Acc16I	TGCGCA	1 cut(s)	1367
Acc65I	GGTACC	1 cut(s)	3501
AccB1I	GGYRCC	3 cut(s)	3278, 3501, 4446
AccB7I	CCANNNNNTGG	3 cut(s)	1697, 1780, 2933
AccBSI	CCGCTC	2 cut(s)	338, 1651
AccI	GTMKAC	1 cut(s)	4150
AccII	CGCG	2 cut(s)	334, 1861
AciI	CCGC	9 cut(s)	338, 1651, 1859, 1902, 2627, 2748, 2918, 4086, 4553
AclI	AACGTT	1 cut(s)	2535
AcoI	YGGCCR	1 cut(s)	4264
AcsI	RAATTY	7 cut(s)	1446, 1512, 1969, 1979, 2102, 2564, 4468
AcuI	CTGAAG	6 cut(s)	1026, 1866, 2133, 2845, 3545, 3741
AcyI	GRCGYC	1 cut(s)	3250
AdeI	CACNNNGTG	2 cut(s)	1562, 2804
AfaI	GTAC	7 cut(s)	160, 950, 2760, 3458, 3503, 4183, 4349
AfiI	CCNNNNNNNGG	8 cut(s)	256, 692, 1697, 1780, 2596, 2933, 3317, 4456
AflII	CTTAAG	1 cut(s)	3883
AflIII	ACRYGT	5 cut(s)	127, 298, 3151, 3453, 3784
AhlI	ACTAGT	1 cut(s)	1129
AjiI	CACGTC	1 cut(s)	4148
AjnI	CCWGG	7 cut(s)	428, 655, 685, 1339, 1940, 2148, 4542
AjuI	GAANNNNNNNTTGG	2 cut(s)	3806, 3838
AleI	CACNNNNGTG	1 cut(s)	2690
Alw21I	GWGCWC	6 cut(s)	974, 1525, 1828, 2679, 3106, 3523
Alw44I	GTGCAC	1 cut(s)	1824
AlwNI	CAGNNNCTG	3 cut(s)	881, 1796, 2960
AoxI	GGCC	5 cut(s)	83, 277, 320, 2587, 4264
ApaLI	GTGCAC	1 cut(s)	1824
ApoI	RAATTY	7 cut(s)	1446, 1512, 1969, 1979, 2102, 2564, 4468
ArsI	GACNNNNNNTTYG	2 cut(s)	3074, 3106
AseI	ATTAAT	1 cut(s)	4092
Asp700I	GAANNNNTTC	4 cut(s)	1612, 1854, 2847, 4173
Asp718I	GGTACC	1 cut(s)	3501
AspLEI	GCGC	7 cut(s)	334, 737, 1368, 1863, 2890, 3052, 4440
AspS9I	GGNCC	6 cut(s)	321, 554, 1928, 2146, 2587, 2818
AsuC2I	CCSGG	1 cut(s)	228
AsuHPI	GGTGA	1 cut(s)	133
AvaII	GGWCC	4 cut(s)	554, 1928, 2146, 2818
AxyI	CCTNAGG	2 cut(s)	2615, 2910
BaeGI	GKGCMC	2 cut(s)	1828, 3151
BalI	TGGCCA	1 cut(s)	4266
BamHI	GGATCC	2 cut(s)	174, 1036
BanI	GGYRCC	3 cut(s)	3278, 3501, 4446
BanII	GRGCYC	3 cut(s)	974, 3106, 3546
BauI	CACGAG	2 cut(s)	2018, 4369
BbsI	GAAGAC	3 cut(s)	2723, 3145, 4129
Bbv12I	GWGCWC	6 cut(s)	974, 1525, 1828, 2679, 3106, 3523
BceAI	ACGGC	3 cut(s)	628, 2025, 2896
BciT130I	CCWGG	7 cut(s)	430, 657, 687, 1341, 1942, 2150, 4544
BciVI	GTATCC	1 cut(s)	3648
BcnI	CCSGG	1 cut(s)	228
BcuI	ACTAGT	1 cut(s)	1129
BfaI	CTAG	8 cut(s)	168, 269, 504, 1130, 1955, 2219, 3654, 4055
BfmI	CTRYAG	2 cut(s)	570, 2739
BfoI	RGCGCY	2 cut(s)	738, 4441
BfrI	CTTAAG	1 cut(s)	3883
BfuI	GTATCC	1 cut(s)	3648
BglI	GCCNNNNNGGC	1 cut(s)	319
BglII	AGATCT	1 cut(s)	3172
BlpI	GCTNAGC	1 cut(s)	2884
BmcAI	AGTACT	1 cut(s)	160
Bme1390I	CCNGG	8 cut(s)	228, 430, 657, 687, 1341, 1942, 2150, 4544
Bme18I	GGWCC	4 cut(s)	554, 1928, 2146, 2818
BmgBI	CACGTC	1 cut(s)	4148
BmgT120I	GGNCC	6 cut(s)	321, 554, 1928, 2146, 2587, 2818
BmrFI	CCNGG	8 cut(s)	228, 430, 657, 687, 1341, 1942, 2150, 4544
BpiI	GAAGAC	3 cut(s)	2723, 3145, 4129
BpmI	CTGGAG	1 cut(s)	3454
Bpu1102I	GCTNAGC	1 cut(s)	2884
BpuEI	CTTGAG	8 cut(s)	2145, 2297, 2778, 3074, 3629, 4130, 4364, 4475
BpuMI	CCSGG	1 cut(s)	228
BsaAI	YACGTR	3 cut(s)	299, 988, 1325
BsaBI	GATNNNNATC	3 cut(s)	2199, 3177, 3693
BsaHI	GRCGYC	1 cut(s)	3250
BsaI	GGTCTC	2 cut(s)	4013, 4366
BsaJI	CCNNGG	5 cut(s)	227, 348, 550, 1941, 2084
BsaWI	WCCGGW	1 cut(s)	584
BsaXI	ACNNNNNCTCC	2 cut(s)	3993, 4023
Bsc4I	CCNNNNNNNGG	8 cut(s)	256, 692, 1697, 1780, 2596, 2933, 3317, 4456
Bse118I	RCCGGY	1 cut(s)	1586
Bse21I	CCTNAGG	2 cut(s)	2615, 2910
Bse3DI	GCAATG	4 cut(s)	198, 1558, 2997, 3691
Bse8I	GATNNNNATC	3 cut(s)	2199, 3177, 3693
BseBI	CCWGG	7 cut(s)	430, 657, 687, 1341, 1942, 2150, 4544
BseDI	CCNNGG	5 cut(s)	227, 348, 550, 1941, 2084
BseJI	GATNNNNATC	3 cut(s)	2199, 3177, 3693
BseLI	CCNNNNNNNGG	8 cut(s)	256, 692, 1697, 1780, 2596, 2933, 3317, 4456
BseMI	GCAATG	4 cut(s)	198, 1558, 2997, 3691
BseMII	CTCAG	6 cut(s)	987, 1392, 1765, 2606, 2898, 2924
BseRI	GAGGAG	3 cut(s)	272, 1079, 3239
BseSI	GKGCMC	2 cut(s)	1828, 3151
BsgI	GTGCAG	3 cut(s)	761, 2172, 2643
Bsh1236I	CGCG	2 cut(s)	334, 1861
BshFI	GGCC	5 cut(s)	85, 279, 322, 2589, 4266
BshNI	GGYRCC	3 cut(s)	3278, 3501, 4446
BsiHKAI	GWGCWC	6 cut(s)	974, 1525, 1828, 2679, 3106, 3523
BsiSI	CCGG	5 cut(s)	227, 468, 585, 1587, 4280
BsiWI	CGTACG	1 cut(s)	4181
BslFI	GGGAC	3 cut(s)	274, 1140, 3076
BslI	CCNNNNNNNGG	8 cut(s)	256, 692, 1697, 1780, 2596, 2933, 3317, 4456
BsmBI	CGTCTC	3 cut(s)	10, 505, 971
BsmFI	GGGAC	3 cut(s)	274, 1140, 3076
BsmI	GAATGC	1 cut(s)	1344
BsnI	GGCC	5 cut(s)	85, 279, 322, 2589, 4266
Bso31I	GGTCTC	2 cut(s)	4013, 4366
Bsp1286I	GDGCHC	8 cut(s)	974, 1525, 1828, 2679, 3106, 3151, 3523, 3546
Bsp1407I	TGTACA	1 cut(s)	948
Bsp1720I	GCTNAGC	1 cut(s)	2884
BspACI	CCGC	9 cut(s)	338, 1651, 1859, 1902, 2627, 2748, 2918, 4086, 4553
BspANI	GGCC	5 cut(s)	85, 279, 322, 2589, 4266
BspCNI	CTCAG	6 cut(s)	986, 1393, 1764, 2607, 2897, 2923
BspFNI	CGCG	2 cut(s)	334, 1861
BspHI	TCATGA	2 cut(s)	2839, 4386
BspMAI	CTGCAG	1 cut(s)	574
BspT107I	GGYRCC	3 cut(s)	3278, 3501, 4446
BspTI	CTTAAG	1 cut(s)	3883
BspTNI	GGTCTC	2 cut(s)	4013, 4366
BsrBI	CCGCTC	2 cut(s)	338, 1651
BsrDI	GCAATG	4 cut(s)	198, 1558, 2997, 3691
BsrFI	RCCGGY	1 cut(s)	1586
BsrGI	TGTACA	1 cut(s)	948
BssAI	RCCGGY	1 cut(s)	1586
BssECI	CCNNGG	5 cut(s)	227, 348, 550, 1941, 2084
BssNI	GRCGYC	1 cut(s)	3250
BssSI	CACGAG	2 cut(s)	2018, 4369
BssT1I	CCWWGG	1 cut(s)	348
Bst2BI	CACGAG	2 cut(s)	2018, 4369
Bst2UI	CCWGG	7 cut(s)	430, 657, 687, 1341, 1942, 2150, 4544
Bst6I	CTCTTC	3 cut(s)	279, 2010, 4179
BstACI	GRCGYC	1 cut(s)	3250
BstAFI	CTTAAG	1 cut(s)	3883
BstAPI	GCANNNNNTGC	1 cut(s)	881
BstAUI	TGTACA	1 cut(s)	948
BstBAI	YACGTR	3 cut(s)	299, 988, 1325
BstDEI	CTNAG	8 cut(s)	973, 1401, 1751, 2210, 2615, 2884, 2910, 4571
BstDSI	CCRYGG	1 cut(s)	550
BstFNI	CGCG	2 cut(s)	334, 1861
BstH2I	RGCGCY	2 cut(s)	738, 4441
BstHHI	GCGC	7 cut(s)	334, 737, 1368, 1863, 2890, 3052, 4440
BstNI	CCWGG	7 cut(s)	430, 657, 687, 1341, 1942, 2150, 4544
BstNSI	RCATGY	5 cut(s)	131, 3033, 3155, 3457, 3788
BstSCI	CCNGG	8 cut(s)	226, 428, 655, 685, 1339, 1940, 2148, 4542
BstSFI	CTRYAG	2 cut(s)	570, 2739
BstSLI	GKGCMC	2 cut(s)	1828, 3151
BstSNI	TACGTA	2 cut(s)	988, 1325
BstUI	CGCG	2 cut(s)	334, 1861
BstV2I	GAAGAC	3 cut(s)	2723, 3145, 4129
BstX2I	RGATCY	5 cut(s)	174, 389, 1036, 2629, 3172
BstYI	RGATCY	5 cut(s)	174, 389, 1036, 2629, 3172
Bsu36I	CCTNAGG	2 cut(s)	2615, 2910
BsuI	GTATCC	1 cut(s)	3648
BsuRI	GGCC	5 cut(s)	85, 279, 322, 2589, 4266
BtgI	CCRYGG	1 cut(s)	550
BtrI	CACGTC	1 cut(s)	4148
BtsI	GCAGTG	2 cut(s)	648, 1911
CaiI	CAGNNNCTG	3 cut(s)	881, 1796, 2960
CciI	TCATGA	2 cut(s)	2839, 4386
CfoI	GCGC	7 cut(s)	334, 737, 1368, 1863, 2890, 3052, 4440
Cfr10I	RCCGGY	1 cut(s)	1586
Cfr13I	GGNCC	6 cut(s)	321, 554, 1928, 2146, 2587, 2818
CseI	GACGC	1 cut(s)	3239
CsiI	ACCWGGT	1 cut(s)	4542
Csp6I	GTAC	7 cut(s)	159, 949, 2759, 3457, 3502, 4182, 4348
CviQI	GTAC	7 cut(s)	159, 949, 2759, 3457, 3502, 4182, 4348
DdeI	CTNAG	8 cut(s)	973, 1401, 1751, 2210, 2615, 2884, 2910, 4571
DraI	TTTAAA	1 cut(s)	90
DraIII	CACNNNGTG	2 cut(s)	1562, 2804
EaeI	YGGCCR	1 cut(s)	4264
Eam1104I	CTCTTC	3 cut(s)	279, 2010, 4179
EarI	CTCTTC	3 cut(s)	279, 2010, 4179
EciI	GGCGGA	1 cut(s)	2763
Ecl136II	GAGCTC	2 cut(s)	972, 3104
Eco105I	TACGTA	2 cut(s)	988, 1325
Eco130I	CCWWGG	1 cut(s)	348
Eco24I	GRGCYC	3 cut(s)	974, 3106, 3546
Eco31I	GGTCTC	2 cut(s)	4013, 4366
Eco32I	GATATC	1 cut(s)	2128
Eco47I	GGWCC	4 cut(s)	554, 1928, 2146, 2818
Eco53kI	GAGCTC	2 cut(s)	972, 3104
Eco57I	CTGAAG	6 cut(s)	1026, 1866, 2133, 2845, 3545, 3741
Eco81I	CCTNAGG	2 cut(s)	2615, 2910
EcoICRI	GAGCTC	2 cut(s)	972, 3104
EcoO109I	RGGNCCY	2 cut(s)	321, 2818
EcoRII	CCWGG	7 cut(s)	428, 655, 685, 1339, 1940, 2148, 4542
EcoRV	GATATC	1 cut(s)	2128
EcoT14I	CCWWGG	1 cut(s)	348
EcoT22I	ATGCAT	4 cut(s)	457, 1112, 3412, 3423
EcoT38I	GRGCYC	3 cut(s)	974, 3106, 3546
ErhI	CCWWGG	1 cut(s)	348
Esp3I	CGTCTC	3 cut(s)	10, 505, 971
FalI	AAGNNNNNCTT	7 cut(s)	2063, 2095, 3126, 3158, 3374, 3406, 4554
FaqI	GGGAC	3 cut(s)	274, 1140, 3076
FauI	CCCGC	2 cut(s)	1644, 2911
FauNDI	CATATG	1 cut(s)	3586
FblI	GTMKAC	1 cut(s)	4150
FriOI	GRGCYC	3 cut(s)	974, 3106, 3546
FspBI	CTAG	8 cut(s)	168, 269, 504, 1130, 1955, 2219, 3654, 4055
FspI	TGCGCA	1 cut(s)	1367
GlaI	GCGC	7 cut(s)	333, 736, 1367, 1862, 2889, 3051, 4439
GsuI	CTGGAG	1 cut(s)	3454
HaeII	RGCGCY	2 cut(s)	738, 4441
HaeIII	GGCC	5 cut(s)	85, 279, 322, 2589, 4266
HapII	CCGG	5 cut(s)	227, 468, 585, 1587, 4280
HgaI	GACGC	1 cut(s)	3239
HhaI	GCGC	7 cut(s)	334, 737, 1368, 1863, 2890, 3052, 4440
Hin1I	GRCGYC	1 cut(s)	3250
Hin6I	GCGC	7 cut(s)	332, 735, 1366, 1861, 2888, 3050, 4438
HinP1I	GCGC	7 cut(s)	332, 735, 1366, 1861, 2888, 3050, 4438
HincII	GTYRAC	1 cut(s)	1162
HindII	GTYRAC	1 cut(s)	1162
HindIII	AAGCTT	3 cut(s)	1693, 2435, 2902
HpaII	CCGG	5 cut(s)	227, 468, 585, 1587, 4280
HphI	GGTGA	1 cut(s)	133
Hpy166II	GTNNAC	6 cut(s)	1162, 1826, 3165, 3959, 4052, 4151
Hpy8I	GTNNAC	6 cut(s)	1162, 1826, 3165, 3959, 4052, 4151
Hpy99I	CGWCG	1 cut(s)	467
HpyCH4IV	ACGT	6 cut(s)	298, 987, 1324, 2535, 3274, 4147
HpyF3I	CTNAG	8 cut(s)	973, 1401, 1751, 2210, 2615, 2884, 2910, 4571
HpySE526I	ACGT	6 cut(s)	298, 987, 1324, 2535, 3274, 4147
Hsp92I	GRCGYC	1 cut(s)	3250
HspAI	GCGC	7 cut(s)	332, 735, 1366, 1861, 2888, 3050, 4438
KpnI	GGTACC	1 cut(s)	3505
LmnI	GCTCC	7 cut(s)	432, 658, 878, 2037, 2976, 3109, 4520
MabI	ACCWGGT	1 cut(s)	4542
MaeI	CTAG	8 cut(s)	168, 269, 504, 1130, 1955, 2219, 3654, 4055
MaeII	ACGT	6 cut(s)	298, 987, 1324, 2535, 3274, 4147
MaeIII	GTNAC	7 cut(s)	242, 807, 1004, 2391, 2452, 3942, 4475
MbiI	CCGCTC	2 cut(s)	338, 1651
MfeI	CAATTG	5 cut(s)	1778, 1986, 2361, 3509, 3663
MflI	RGATCY	5 cut(s)	174, 389, 1036, 2629, 3172
MhlI	GDGCHC	8 cut(s)	974, 1525, 1828, 2679, 3106, 3151, 3523, 3546
MlsI	TGGCCA	1 cut(s)	4266
MluNI	TGGCCA	1 cut(s)	4266
MlyI	GAGTC	7 cut(s)	296, 480, 745, 1412, 2192, 3098, 4550
MmeI	TCCRAC	7 cut(s)	540, 584, 1014, 1122, 1359, 1683, 3686
Mox20I	TGGCCA	1 cut(s)	4266
Mph1103I	ATGCAT	4 cut(s)	457, 1112, 3412, 3423
MroXI	GAANNNNTTC	4 cut(s)	1612, 1854, 2847, 4173
MscI	TGGCCA	1 cut(s)	4266
MslI	CAYNNNNRTG	5 cut(s)	798, 1155, 1353, 2690, 3949
Msp20I	TGGCCA	1 cut(s)	4266
MspA1I	CMGCKG	3 cut(s)	2357, 2627, 4086
MspCI	CTTAAG	1 cut(s)	3883
MspI	CCGG	5 cut(s)	227, 468, 585, 1587, 4280
MspR9I	CCNGG	8 cut(s)	228, 430, 657, 687, 1341, 1942, 2150, 4544
MunI	CAATTG	5 cut(s)	1778, 1986, 2361, 3509, 3663
Mva1269I	GAATGC	1 cut(s)	1344
MvaI	CCWGG	7 cut(s)	430, 657, 687, 1341, 1942, 2150, 4544
MvnI	CGCG	2 cut(s)	334, 1861
NciI	CCSGG	1 cut(s)	228
NdeI	CATATG	1 cut(s)	3586
NmuCI	GTSAC	3 cut(s)	2452, 3942, 4475
NsbI	TGCGCA	1 cut(s)	1367
NsiI	ATGCAT	4 cut(s)	457, 1112, 3412, 3423
NspI	RCATGY	5 cut(s)	131, 3033, 3155, 3457, 3788
OliI	CACNNNNGTG	1 cut(s)	2690
PagI	TCATGA	2 cut(s)	2839, 4386
PciI	ACATGT	4 cut(s)	127, 3151, 3453, 3784
PctI	GAATGC	1 cut(s)	1344
PdmI	GAANNNNTTC	4 cut(s)	1612, 1854, 2847, 4173
Pfl23II	CGTACG	1 cut(s)	4181
PflFI	GACNNNGTC	1 cut(s)	1402
PflMI	CCANNNNNTGG	3 cut(s)	1697, 1780, 2933
PfoI	TCCNGGA	2 cut(s)	428, 685
PleI	GAGTC	7 cut(s)	295, 479, 745, 1411, 2191, 3097, 4550
PpsI	GAGTC	7 cut(s)	295, 479, 745, 1411, 2191, 3097, 4550
Ppu21I	YACGTR	3 cut(s)	299, 988, 1325
PpuMI	RGGWCCY	1 cut(s)	2818
PscI	ACATGT	4 cut(s)	127, 3151, 3453, 3784
PshBI	ATTAAT	1 cut(s)	4092
PsiI	TTATAA	2 cut(s)	1386, 3257
Psp124BI	GAGCTC	2 cut(s)	974, 3106
Psp1406I	AACGTT	1 cut(s)	2535
Psp5II	RGGWCCY	1 cut(s)	2818
Psp6I	CCWGG	7 cut(s)	428, 655, 685, 1339, 1940, 2148, 4542
PspGI	CCWGG	7 cut(s)	428, 655, 685, 1339, 1940, 2148, 4542
PspLI	CGTACG	1 cut(s)	4181
PspPI	GGNCC	6 cut(s)	321, 554, 1928, 2146, 2587, 2818
PspPPI	RGGWCCY	1 cut(s)	2818
PstI	CTGCAG	1 cut(s)	574
PstNI	CAGNNNCTG	3 cut(s)	881, 1796, 2960
PsuI	RGATCY	5 cut(s)	174, 389, 1036, 2629, 3172
PsyI	GACNNNGTC	1 cut(s)	1402
PvuII	CAGCTG	1 cut(s)	2357
RsaI	GTAC	7 cut(s)	160, 950, 2760, 3458, 3503, 4183, 4349
RsaNI	GTAC	7 cut(s)	159, 949, 2759, 3457, 3502, 4182, 4348
RseI	CAYNNNNRTG	5 cut(s)	798, 1155, 1353, 2690, 3949
SacI	GAGCTC	2 cut(s)	974, 3106
Sau96I	GGNCC	6 cut(s)	321, 554, 1928, 2146, 2587, 2818
ScaI	AGTACT	1 cut(s)	160
SchI	GAGTC	7 cut(s)	296, 480, 745, 1412, 2192, 3098, 4550
ScrFI	CCNGG	8 cut(s)	228, 430, 657, 687, 1341, 1942, 2150, 4544
SduI	GDGCHC	8 cut(s)	974, 1525, 1828, 2679, 3106, 3151, 3523, 3546
SexAI	ACCWGGT	1 cut(s)	4542
SfcI	CTRYAG	2 cut(s)	570, 2739
SinI	GGWCC	4 cut(s)	554, 1928, 2146, 2818
SmiMI	CAYNNNNRTG	5 cut(s)	798, 1155, 1353, 2690, 3949
SmlI	CTYRAG	9 cut(s)	2160, 2276, 2793, 3053, 3608, 3883, 4109, 4343, 4454
SmoI	CTYRAG	9 cut(s)	2160, 2276, 2793, 3053, 3608, 3883, 4109, 4343, 4454
SnaBI	TACGTA	2 cut(s)	988, 1325
SpeI	ACTAGT	1 cut(s)	1129
SsiI	CCGC	9 cut(s)	338, 1651, 1859, 1902, 2627, 2748, 2918, 4086, 4553
SspI	AATATT	4 cut(s)	1272, 1497, 3016, 3767
SspMI	CTAG	8 cut(s)	168, 269, 504, 1130, 1955, 2219, 3654, 4055
SstI	GAGCTC	2 cut(s)	974, 3106
StyD4I	CCNGG	8 cut(s)	226, 428, 655, 685, 1339, 1940, 2148, 4542
StyI	CCWWGG	1 cut(s)	348
TaiI	ACGT	6 cut(s)	301, 990, 1327, 2538, 3277, 4150
TaqI	TCGA	2 cut(s)	4197, 4315
TaqII	GACCGA	1 cut(s)	4140
TatI	WGTACW	3 cut(s)	158, 948, 4347
TauI	GCSGC	1 cut(s)	1904
TseFI	GTSAC	3 cut(s)	2452, 3942, 4475
Tsp45I	GTSAC	3 cut(s)	2452, 3942, 4475
TspGWI	ACGGA	3 cut(s)	567, 812, 4199
Tth111I	GACNNNGTC	1 cut(s)	1402
Van91I	CCANNNNNTGG	3 cut(s)	1697, 1780, 2933
Vha464I	CTTAAG	1 cut(s)	3883
VneI	GTGCAC	1 cut(s)	1824
VpaK11BI	GGWCC	4 cut(s)	554, 1928, 2146, 2818
VspI	ATTAAT	1 cut(s)	4092
XapI	RAATTY	7 cut(s)	1446, 1512, 1969, 1979, 2102, 2564, 4468
XceI	RCATGY	5 cut(s)	131, 3033, 3155, 3457, 3788
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3588, 4410
XmiI	GTMKAC	1 cut(s)	4150
XmnI	GAANNNNTTC	4 cut(s)	1612, 1854, 2847, 4173
XspI	CTAG	8 cut(s)	168, 269, 504, 1130, 1955, 2219, 3654, 4055
ZrmI	AGTACT	1 cut(s)	160
Zsp2I	ATGCAT	4 cut(s)	457, 1112, 3412, 3423

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.