Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0002601

Physical Location & Seq

Genomic Coordinates Forward (+)

46617041 .. 46645855

Gene Structure 28815 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000009106

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4521 bp

ATGACCGCTGCTAACGATGGAGACATAGCTATTGCTCGAGCACTTTTGCAGCTACTGTCGATCGTCAACAATATCCTTTCTTCAACTGAAATTGTGGAAGAAATGAGGAGTTGGAATACAAGAGTCGGCTATAGGCGACTTGAATGCAAACTATGTTACCGTTGTGTCCCCTATGTTGGGGCGGATTTCTCGATTTTTGGGGATGACACCAAGAATCTGTTCTCATGGAGAATCCACAAGGATTTCCCCCGTACTCTCGAATTACTCGAGCTCTCTGCTAAGAAAGAATTTCCAAAAGTCGGACTGCGAATCACTTTTCCAGACGCACTCTCTTCTTCAGTTTTTCTTTGCAAAAATGAGATTGATATCGGTTCCAGGAGCCACCCCTATTTGCTTTATGCATTGACTGTGGCCAGAGTCGCTTACCTTTTGAAACTTGGGACCACCTCTAGCTATGCACCCATCTCTGTTATTAGAGAGTTTAACTTGCATCCCTACGGACCTATTAGCAGTGCCGCAGCAACGCCGACTGGATGTCTTGTTGTAGGTCGGAATGACGGCTCACTTGCTTGCTTTCAACTCACACTTGAATCCACTGCTCCTGGTTTTCTGCAAGAGCTACGGGATGATTCAGGAACTGGTAGTTTCTGGGGTTTCATGTCAAGGGGTAGGACGGTAGGGGCTGTGCAGGACTTGGTTATATCTATGGTTCATGGGAAACCACTTATATTTGTGATTCATACGGATTGGGTGTTAAGAGTATGGGATCTTTCATTTCATGGCAGGCTCTTTGCCAATAAGATGAAAGGTCCAACAGTGGCAGGAGCTGCATTGGTAAGGTTGTGGGTTGGTCAAGCTGACAATGACTCGAGCATAATTCCTGTGGCGATCTTGTACAGGCATAATTCGGATATTAGCTCAGAGACAATCTACGTATATAGGCTCCATTGTAACGTGAGAGACAGAATTGAATTATTGATATATCGTTCAAAACAAAAAATTCCTATTGAGGAGGATGAATGGATTGATGTCAAACTTATTTCCAACAAGATATGCATATTGAAAAATGACGGACTGGTGTTTCTCAAGCTGTTGCATATAAATGCCGAGACGGTAGATGCAGTTTGTTATCCTTTGCAAGAGGACTTTGTTGCAGATCAACTCTTTCAAAGTTCAGAGCATTCTTCGGATAACCTTCTTTTGATAACTCATTCAATACTTTCATCTTCAAGGGATCATATTCTACCCGTTGTATCTTCTATTTTCTTACGTAGGCTGCTTCTTCCTGGCATTCATCATAATGCTGCTTTGCGCACAACTTTGTTGGATTATAATAGGCACTGGACTGAGTCAGATTTCCACTCCTTAACTGCTGATGGGTTGAAGAAGGAAATTCTTTCCCTGATTGAACATGAGGTTCTGACTGGTAATTCATCGTCAATACTTTGCTGCTGGAAAATTTTTTGTGCTCGCTATTTCCAAAACTGGTGCAAGAGCAATGCACCATGTGGCCTGCTTGTTGATTCCTCAACCGGCACTGTTGGTTTAATTAGAAAGAGTTCAGTATCTTTTTTTCGTTCTTTGGAGGATATTGAGCGGGTTAATGATGGCTCTTTGGATGAACTTGGCAATATTCCAAGCTTTGGGTTGGATTCATTTGGCAAGGCTGATTGTAAGCTACTTGCTGAAATGCTCAGGTGTATCACTAATGTCAGCCAGCAATTGGGAAAAACAGCTTCTGCTATATTTTACGAATTACTCATTAGTGCACCACCAGTTATCTCATCTAAAGAAATTATTCCACGCCTACTGAAGATATTAGAAACTGGATATAGTTCAACAGCAGCAGCGCTTTGGATAGCAGATCTTGGAATTGATCTTGCCTGGCAGAAAAATCTAGCAGATCACAAAAATTTGAGGAAATTTTCTATTGACATGATGAAGTCTCTTCATGCTTTGCACGAGAAATCTGGCACATGGAGCTGTATTTTAAGTGTGATTGAGAAGTATCTTAAGAATCTCATACCTCGAAAAATTATACAAAAATTTGATGCTGAAGTAGCATTGGATATTAATGCCTCCATCTTGGTCCCGGCTACGTTTCAAGTTGCAAAGGTTATGTTTGAGTCTGCATTGGATATTCATCTGTTTCTAAGCTATCTAGTGAGCATTAGCAGGCAGATCAATATGCTACATGATGATATATCCAAAATACAACTTGAGTTGGTGCCAATGATTCAAGAAGTTATTTATGAGTGGCTTCTCCTGCATTTCTTTGCCACCACACCATCTGAATCAGCTGCAATTGAAGATTTCAGCTCTCAACTTTCATTGTTACAAATTGATAGCAACACTGGTAGAAGATTATGGAATGAGAAGCTTGGGAAGTGCGAGTTCACTCTGGCTTTTATATTCTTACTTAATGTTAGAAGTTCCTCCAAAGACCAGAAGCACTTTTCTCCAAGAAGCATACCAAGCGTTGAGGACATAATTGATTCAGTACGAGAGTTTGATAGCTGGACCATGTGGGGCCAGGATGGGGAATCCCTTACTGTCCTGAAGCGTGCAGTGGATCTTGCATTGATCCTGCTAAGGCACGGGCAGTATGATGCTGTTGAGCACTTGCTCACTATTGTGGAGGCACATTTAGAAAAGGAGGAGACTTCTCACGGTATTAGAGCTACAGATGGTGGATGGTGCATACTTCATCATCTTTTAGGACGCTGCTTCCTTGCTCAGGCACATCGTGGAGTACATGGGGTTCTGAAAGAGAGGAAAGTTCATGAAGCTGTTCGTTGTTTCTTCAGAGCCGCATCTGGAAACGGGTCAGCTCATGCTTTACAAAGCTTGCCTCAGGAAGCGTGGTTGCCACATCTTGGTATCGATGGTAGCGTATCAGATGCTGCATGGAGGCTCCATTACTATCAGTGGGCAATGCAGATATGTGAGCATTATAATATTAGTGAAGGTGCATGTCAGTTTGCTCTTGCTGCGCTTGAGCAAGTTGAGGAAGCTTACAGTGCAAACAATGAGTCCCATGACAGAGCTCCATTTATTGTATCAGTATCTACCATCAAAGGAAGACTTTGGGAAAATATTTGCAAATTTACTTTAGATCTCAATCTCTATTATGATGCATATTGTGCAATAATTTCAAATCCAGATGAGGAGAGCAAGTACATCTGCTTGAGGCGTCTTATAAATGTTCTTTATGAACGTGGTGCCATAAAGATTCTATGTGGTGGTCAGCTACCATTCATTGGTCTTACAGAGAAGGTAGAACAAGAGCTTGCATGGAAGGCAGAGCGGTCAGACATTTTAGCAAAGACAAACTTATATAAGTTGCTTTATGCATTTGAAATGCATCGGCATAACTGGCGAAAAGCAGCAAGCTACATGTACCTTTATTCTGCCCGTTTGAGAACTGAAGCATCTCTGAAAGGTTACCAACAATTGTGGCGAGTACTGAAGGAGATACTGAATGGGCTCTCTGCTGCTATCAACGCACTTCATCTTGTTCATCCAGCATATGCTTGGATTGATCCTCTGCTTGCGAGAAGTGCTCTTCGCAACGAGCAGTATCCAGGTAGAGAGGCTAGAATAACAATTGAAGACCAGCCAGCAAACAATGATGTTCATCCTCGAAGTTGGAAATCTTTTATAGATATTCAGAAAATTGAGAACGAGTTTGTGCTTACTTCAGCAGAACATCTGCTTTCACTGGCACATGTCAAATGGACAAATACAGGAACCCAGACAAAACCATTAGAGTTGGTTGATCTTTTGATCCAGACAAATCTATATGATATGGCATTTACAGTTCTTGTAAGATTCTTCAAAGGTTCAGAGTTAAAGAGGGGATTGGAAAGAGTTTTCTCAGCTATGTCACTAAAATGCTTTCCACATATAGTTGATTCTTCAAGGGTTGGGGATGATCCCATCGTTCATGGTTCACCAGATATGTCTTCAGCAAATCAACAATCCAATGGAATAAATCAATGGGGAACTCTCGAGCTTTATCTTGACCGGTATAAAGTGTTTCATGCACGTCTACCACTAATTGTTGCCGAAACTCTTCTTCGTACGGATTCTCAAATCCAACTGCCTCTTTGGTTGGTTAAATTATTCAAGGATGGCAGGAGGGAAAAGACATTGGGGATGACTGGCCACGAATCAAATCCGGCATCGTTGTTTCAATTATATGTTGATTATCGTCGATATGGAGAAGCTATAAATCTGTTGCTCGAGCACAAAGGGTGTTTTGGATCCATGAGACCAGCCAACATCATGAATCGTAAAAGACCATTTGGAGTTTGGTTTCCGTATAGAATGATTCAGCGCCTCTGGTGCCAACTTGAGGAAATGATAAATTCCGGTCAAATGGTAGATCAATGTAAGCAGTTCAAAGATTTGCTACACAGAGCTTTGCTGAAACATCTCCAACTGGAAAAGATGGACTCGGAAGAAGCACTTAGTTTTATTTCAGAAAATAAGATGGAAACACTTCGGATGCAATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0008104 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016973 GO:0031090 GO:0031503 GO:0031965 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0034613 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

DUF5311 Nup160

Protein Analysis

1507

Amino Acids

170.93

Weight (kDa)

6.72

Isoelectric Point (pI)

41.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_Nup120_160	PF11715	74 - 532	9.7e-54	Nucleoporin Nup120/160, beta-propeller domain
NUP160_helical_2	PF17238	555 - 742	1.5e-81	NUP160, helical domain
TPR_NUP160_120_M	PF23354	921 - 1172	3.6e-35	NUP160/120 middle TPR
HEAT_Nup120	PF23300	922 - 1072	1.9e-12	Nucleoporin Nup120, HEAT repeats
TPR_Nup160_C	PF23347	1224 - 1473	4.7e-08	Nup160 C-terminal TPR

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002601)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33410 AT1G33410
fragaria_vesca	FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400 FvH4_6g26400
malus_domestica	MD00G1034800.v1.1 MD05G1063300.v1.1 MD05G1161100.v1.1 MD05G1162300.v1.1 MD05G1162400.v1.1 MD07G1280900.v1.1 MD08G1158600.v1.1 MD10G1009800.v1.1 MD10G1160000.v1.1
prunus_persica	Prupe.5G022000_v2.0.a1
pyrus_communis	pycom10g00600
rosa_chinensis	RchiOBHm_Chr2g0126921 RchiOBHm_Chr4g0402211
rosa_laevigata	RLG00000009106 RLG00000018928
rosa_multiflora	Rmu_sc0000007.1_g000030 Rmu_sc0001405.1_g000051
rosa_roxburghii	Rroxscaffold_1G00047890 Rroxscaffold_2G00116920 Rroxscaffold_5G00346940
rosa_rugosa	Rorug02G0270100 Rorug02G0270200
rosa_samantha	Rh2AG322500 Rh2BG330700 Rh2CG310600 Rh2DG350000 Rh4BG104100 Rh4CG117900 Rh4DG101500
rosa_wichuraiana	Rw2G026160 Rw4G008910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1332, 2955, 3200
Acc16I	TGCGCA	1 cut(s)	1313
AccB1I	GGYRCC	3 cut(s)	2227, 3221, 4347
AccB7I	CCANNNNNTGG	4 cut(s)	1643, 1723, 2876, 3260
AccBSI	CCGCTC	2 cut(s)	1597, 3307
AccI	GTMKAC	1 cut(s)	4051
AciI	CCGC	6 cut(s)	6, 182, 516, 1597, 2811, 3307
AclWI	GGATC	9 cut(s)	774, 1242, 2578, 2580, 3536, 3781, 3929, 4260, 4273
AcoI	YGGCCR	2 cut(s)	411, 4165
AcsI	RAATTY	9 cut(s)	287, 999, 1392, 1458, 1912, 1922, 2045, 3104, 4369
AcuI	CTGAAG	9 cut(s)	321, 1832, 2076, 2579, 2788, 3447, 3488, 3684, 3951
AcyI	GRCGYC	1 cut(s)	3193
AdeI	CACNNNGTG	2 cut(s)	1508, 2747
AfaI	GTAC	8 cut(s)	253, 896, 2502, 2754, 3179, 3401, 3465, 4084
AfeI	AGCGCT	1 cut(s)	1851
AflII	CTTAAG	1 cut(s)	2012
AflIII	ACRYGT	2 cut(s)	3396, 3727
AgeI	ACCGGT	1 cut(s)	4026
AhdI	GACNNNNNGTC	1 cut(s)	534
AjiI	CACGTC	1 cut(s)	4049
AjnI	CCWGG	6 cut(s)	374, 601, 1285, 1883, 2532, 3583
AjuI	GAANNNNNNNTTGG	2 cut(s)	203, 235
AleI	CACNNNNGTG	1 cut(s)	2633
Alw21I	GWGCWC	8 cut(s)	43, 273, 1471, 1771, 2622, 3049, 3565, 4251
Alw26I	GTCTC	7 cut(s)	15, 917, 954, 1103, 1950, 2654, 4267
Alw44I	GTGCAC	1 cut(s)	1767
AlwI	GGATC	9 cut(s)	774, 1242, 2578, 2580, 3536, 3781, 3929, 4260, 4273
AlwNI	CAGNNNCTG	5 cut(s)	55, 638, 827, 1739, 2903
Ama87I	CYCGRG	5 cut(s)	36, 266, 868, 4010, 4244
Aor51HI	AGCGCT	1 cut(s)	1851
AoxI	GGCC	4 cut(s)	411, 1510, 2530, 4165
ApaLI	GTGCAC	1 cut(s)	1767
ApoI	RAATTY	9 cut(s)	287, 999, 1392, 1458, 1912, 1922, 2045, 3104, 4369
ArsI	GACNNNNNNTTYG	2 cut(s)	3017, 3049
AseI	ATTAAT	1 cut(s)	2073
AsiGI	ACCGGT	1 cut(s)	4026
Asp700I	GAANNNNTTC	6 cut(s)	218, 1558, 1797, 2790, 4074, 4503
AspLEI	GCGC	4 cut(s)	1314, 1852, 2995, 4341
AspS9I	GGNCC	6 cut(s)	441, 500, 809, 2089, 2520, 2530
AsuC2I	CCSGG	1 cut(s)	2093
AsuHPI	GGTGA	1 cut(s)	3945
AvaI	CYCGRG	5 cut(s)	36, 266, 868, 4010, 4244
AvaII	GGWCC	5 cut(s)	441, 500, 809, 2089, 2520
AxyI	CCTNAGG	1 cut(s)	2853
BaeGI	GKGCMC	1 cut(s)	1771
BalI	TGGCCA	2 cut(s)	413, 4167
BamHI	GGATCC	1 cut(s)	4265
BanI	GGYRCC	3 cut(s)	2227, 3221, 4347
BanII	GRGCYC	3 cut(s)	273, 3049, 3489
BauI	CACGAG	1 cut(s)	1961
BbsI	GAAGAC	3 cut(s)	3088, 3618, 3957
Bbv12I	GWGCWC	8 cut(s)	43, 273, 1471, 1771, 2622, 3049, 3565, 4251
BceAI	ACGGC	1 cut(s)	574
BcgI	CGANNNNNNTGC	4 cut(s)	126, 160, 257, 291
BciT130I	CCWGG	6 cut(s)	376, 603, 1287, 1885, 2534, 3585
BciVI	GTATCC	1 cut(s)	3591
BcnI	CCSGG	1 cut(s)	2093
BcoDI	GTCTC	7 cut(s)	15, 917, 954, 1103, 1950, 2654, 4267
BfaI	CTAG	4 cut(s)	450, 1898, 2162, 3597
BfmI	CTRYAG	2 cut(s)	130, 2682
BfoI	RGCGCY	2 cut(s)	1853, 4342
BfrI	CTTAAG	1 cut(s)	2012
BfuI	GTATCC	1 cut(s)	3591
BglII	AGATCT	2 cut(s)	1864, 3115
BmcAI	AGTACT	1 cut(s)	3465
Bme1390I	CCNGG	7 cut(s)	376, 603, 1287, 1885, 2093, 2534, 3585
Bme18I	GGWCC	5 cut(s)	441, 500, 809, 2089, 2520
BmeRI	GACNNNNNGTC	1 cut(s)	534
BmeT110I	CYCGRG	5 cut(s)	36, 266, 868, 4010, 4244
BmgBI	CACGTC	1 cut(s)	4049
BmgT120I	GGNCC	6 cut(s)	441, 500, 809, 2089, 2520, 2530
BmrFI	CCNGG	7 cut(s)	376, 603, 1287, 1885, 2093, 2534, 3585
BpiI	GAAGAC	3 cut(s)	3088, 3618, 3957
BplI	GAGNNNNNCTC	2 cut(s)	3547, 3579
Bpu10I	CCTNAGC	3 cut(s)	1694, 2591, 2736
BpuEI	CTTGAG	5 cut(s)	1070, 2240, 3017, 3208, 4376
BpuMI	CCSGG	1 cut(s)	2093
Bsa29I	ATCGAT	1 cut(s)	2883
BsaAI	YACGTR	2 cut(s)	934, 1271
BsaBI	GATNNNNATC	4 cut(s)	365, 2142, 3120, 3636
BsaHI	GRCGYC	1 cut(s)	3193
BsaI	GGTCTC	1 cut(s)	4267
BsaWI	WCCGGW	2 cut(s)	4026, 4373
BsaXI	ACNNNNNCTCC	2 cut(s)	3161, 3191
Bse118I	RCCGGY	2 cut(s)	1532, 4026
Bse21I	CCTNAGG	1 cut(s)	2853
Bse3DI	GCAATG	2 cut(s)	1504, 2940
Bse8I	GATNNNNATC	4 cut(s)	365, 2142, 3120, 3636
BseBI	CCWGG	6 cut(s)	376, 603, 1287, 1885, 2534, 3585
BseCI	ATCGAT	1 cut(s)	2883
BseJI	GATNNNNATC	4 cut(s)	365, 2142, 3120, 3636
BseMI	GCAATG	2 cut(s)	1504, 2940
BseMII	CTCAG	6 cut(s)	933, 1338, 1708, 2750, 2867, 3891
BseRI	GAGGAG	4 cut(s)	121, 1025, 2672, 3182
BseSI	GKGCMC	1 cut(s)	1771
BsgI	GTGCAG	2 cut(s)	707, 2586
Bsh1285I	CGRYCG	1 cut(s)	63
BshFI	GGCC	4 cut(s)	413, 1512, 2532, 4167
BshNI	GGYRCC	3 cut(s)	2227, 3221, 4347
BshTI	ACCGGT	1 cut(s)	4026
BshVI	ATCGAT	1 cut(s)	2883
BsiEI	CGRYCG	1 cut(s)	63
BsiHKAI	GWGCWC	8 cut(s)	43, 273, 1471, 1771, 2622, 3049, 3565, 4251
BsiHKCI	CYCGRG	5 cut(s)	36, 266, 868, 4010, 4244
BsiSI	CCGG	5 cut(s)	1533, 2093, 4027, 4181, 4374
BsiWI	CGTACG	1 cut(s)	4082
BslFI	GGGAC	4 cut(s)	152, 454, 2075, 3019
BsmAI	GTCTC	7 cut(s)	15, 917, 954, 1103, 1950, 2654, 4267
BsmBI	CGTCTC	1 cut(s)	1103
BsmFI	GGGAC	4 cut(s)	152, 454, 2075, 3019
BsmI	GAATGC	3 cut(s)	149, 1180, 1290
BsnI	GGCC	4 cut(s)	413, 1512, 2532, 4167
Bso31I	GGTCTC	1 cut(s)	4267
BsoBI	CYCGRG	5 cut(s)	36, 266, 868, 4010, 4244
Bsp1286I	GDGCHC	9 cut(s)	43, 273, 1471, 1771, 2622, 3049, 3489, 3565, 4251
Bsp1407I	TGTACA	1 cut(s)	894
BspACI	CCGC	6 cut(s)	6, 182, 516, 1597, 2811, 3307
BspANI	GGCC	4 cut(s)	413, 1512, 2532, 4167
BspCNI	CTCAG	6 cut(s)	932, 1339, 1707, 2749, 2866, 3890
BspDI	ATCGAT	1 cut(s)	2883
BspHI	TCATGA	2 cut(s)	2782, 4287
BspPI	GGATC	9 cut(s)	774, 1242, 2578, 2580, 3536, 3781, 3929, 4260, 4273
BspQI	GCTCTTC	1 cut(s)	3570
BspT107I	GGYRCC	3 cut(s)	2227, 3221, 4347
BspTI	CTTAAG	1 cut(s)	2012
BspTNI	GGTCTC	1 cut(s)	4267
BsrBI	CCGCTC	2 cut(s)	1597, 3307
BsrDI	GCAATG	2 cut(s)	1504, 2940
BsrFI	RCCGGY	2 cut(s)	1532, 4026
BsrGI	TGTACA	1 cut(s)	894
BssAI	RCCGGY	2 cut(s)	1532, 4026
BssNI	GRCGYC	1 cut(s)	3193
BssSI	CACGAG	1 cut(s)	1961
Bst2BI	CACGAG	1 cut(s)	1961
Bst2UI	CCWGG	6 cut(s)	376, 603, 1287, 1885, 2534, 3585
Bst6I	CTCTTC	4 cut(s)	337, 1953, 3570, 4080
BstACI	GRCGYC	1 cut(s)	3193
BstAFI	CTTAAG	1 cut(s)	2012
BstAPI	GCANNNNNTGC	2 cut(s)	827, 3143
BstAUI	TGTACA	1 cut(s)	894
BstBAI	YACGTR	2 cut(s)	934, 1271
BstEII	GGTNACC	1 cut(s)	3443
BstENI	CCTNNNNNAGG	1 cut(s)	2735
BstH2I	RGCGCY	2 cut(s)	1853, 4342
BstHHI	GCGC	4 cut(s)	1314, 1852, 2995, 4341
BstMAI	GTCTC	7 cut(s)	15, 917, 954, 1103, 1950, 2654, 4267
BstMCI	CGRYCG	1 cut(s)	63
BstNI	CCWGG	6 cut(s)	376, 603, 1287, 1885, 2534, 3585
BstNSI	RCATGY	3 cut(s)	2976, 3400, 3731
BstPI	GGTNACC	1 cut(s)	3443
BstSCI	CCNGG	7 cut(s)	374, 601, 1285, 1883, 2091, 2532, 3583
BstSFI	CTRYAG	2 cut(s)	130, 2682
BstSLI	GKGCMC	1 cut(s)	1771
BstSNI	TACGTA	2 cut(s)	934, 1271
BstV2I	GAAGAC	3 cut(s)	3088, 3618, 3957
BstX2I	RGATCY	5 cut(s)	766, 1864, 2572, 3115, 4265
BstYI	RGATCY	5 cut(s)	766, 1864, 2572, 3115, 4265
Bsu15I	ATCGAT	1 cut(s)	2883
Bsu36I	CCTNAGG	1 cut(s)	2853
BsuI	GTATCC	1 cut(s)	3591
BsuRI	GGCC	4 cut(s)	413, 1512, 2532, 4167
BsuTUI	ATCGAT	1 cut(s)	2883
BtrI	CACGTC	1 cut(s)	4049
BtsI	GCAGTG	3 cut(s)	517, 594, 2574
CaiI	CAGNNNCTG	5 cut(s)	55, 638, 827, 1739, 2903
CciI	TCATGA	2 cut(s)	2782, 4287
CfoI	GCGC	4 cut(s)	1314, 1852, 2995, 4341
Cfr10I	RCCGGY	2 cut(s)	1532, 4026
Cfr13I	GGNCC	6 cut(s)	441, 500, 809, 2089, 2520, 2530
ClaI	ATCGAT	1 cut(s)	2883
CseI	GACGC	3 cut(s)	332, 2730, 3182
Csp6I	GTAC	8 cut(s)	252, 895, 2501, 2753, 3178, 3400, 3464, 4083
CspAI	ACCGGT	1 cut(s)	4026
CspCI	CAANNNNNGTGG	2 cut(s)	3437, 3472
CviQI	GTAC	8 cut(s)	252, 895, 2501, 2753, 3178, 3400, 3464, 4083
DraIII	CACNNNGTG	2 cut(s)	1508, 2747
DriI	GACNNNNNGTC	1 cut(s)	534
EaeI	YGGCCR	2 cut(s)	411, 4165
Eam1104I	CTCTTC	4 cut(s)	337, 1953, 3570, 4080
Eam1105I	GACNNNNNGTC	1 cut(s)	534
EarI	CTCTTC	4 cut(s)	337, 1953, 3570, 4080
EciI	GGCGGA	1 cut(s)	197
Ecl136II	GAGCTC	2 cut(s)	271, 3047
Eco105I	TACGTA	2 cut(s)	934, 1271
Eco24I	GRGCYC	3 cut(s)	273, 3049, 3489
Eco31I	GGTCTC	1 cut(s)	4267
Eco32I	GATATC	1 cut(s)	367
Eco47I	GGWCC	5 cut(s)	441, 500, 809, 2089, 2520
Eco47III	AGCGCT	1 cut(s)	1851
Eco53kI	GAGCTC	2 cut(s)	271, 3047
Eco57I	CTGAAG	9 cut(s)	321, 1832, 2076, 2579, 2788, 3447, 3488, 3684, 3951
Eco81I	CCTNAGG	1 cut(s)	2853
Eco88I	CYCGRG	5 cut(s)	36, 266, 868, 4010, 4244
Eco91I	GGTNACC	1 cut(s)	3443
EcoICRI	GAGCTC	2 cut(s)	271, 3047
EcoNI	CCTNNNNNAGG	1 cut(s)	2735
EcoO65I	GGTNACC	1 cut(s)	3443
EcoRII	CCWGG	6 cut(s)	374, 601, 1285, 1883, 2532, 3583
EcoRV	GATATC	1 cut(s)	367
EcoT22I	ATGCAT	5 cut(s)	403, 1058, 3139, 3355, 3366
EcoT38I	GRGCYC	3 cut(s)	273, 3049, 3489
Esp3I	CGTCTC	1 cut(s)	1103
FalI	AAGNNNNNCTT	6 cut(s)	3069, 3101, 3317, 3349, 4455, 4487
FaqI	GGGAC	4 cut(s)	152, 454, 2075, 3019
FauI	CCCGC	1 cut(s)	1590
FauNDI	CATATG	1 cut(s)	3529
FblI	GTMKAC	1 cut(s)	4051
FriOI	GRGCYC	3 cut(s)	273, 3049, 3489
FspBI	CTAG	4 cut(s)	450, 1898, 2162, 3597
FspI	TGCGCA	1 cut(s)	1313
GlaI	GCGC	4 cut(s)	1313, 1851, 2994, 4340
HaeII	RGCGCY	2 cut(s)	1853, 4342
HaeIII	GGCC	4 cut(s)	413, 1512, 2532, 4167
HapII	CCGG	5 cut(s)	1533, 2093, 4027, 4181, 4374
HgaI	GACGC	3 cut(s)	332, 2730, 3182
HhaI	GCGC	4 cut(s)	1314, 1852, 2995, 4341
Hin1I	GRCGYC	1 cut(s)	3193
Hin6I	GCGC	4 cut(s)	1312, 1850, 2993, 4339
HinP1I	GCGC	4 cut(s)	1312, 1850, 2993, 4339
HincII	GTYRAC	1 cut(s)	67
HindII	GTYRAC	1 cut(s)	67
HindIII	AAGCTT	4 cut(s)	1639, 2378, 2845, 3012
HpaII	CCGG	5 cut(s)	1533, 2093, 4027, 4181, 4374
HphI	GGTGA	1 cut(s)	3945
Hpy166II	GTNNAC	5 cut(s)	67, 1769, 2397, 3953, 4052
Hpy8I	GTNNAC	5 cut(s)	67, 1769, 2397, 3953, 4052
Hpy99I	CGWCG	1 cut(s)	4218
HpyAV	CCTTC	6 cut(s)	1205, 1381, 2960, 3268, 3292, 3463
HpyCH4IV	ACGT	6 cut(s)	933, 954, 1270, 2099, 3217, 4048
HpySE526I	ACGT	6 cut(s)	933, 954, 1270, 2099, 3217, 4048
Hsp92I	GRCGYC	1 cut(s)	3193
HspAI	GCGC	4 cut(s)	1312, 1850, 2993, 4339
LguI	GCTCTTC	1 cut(s)	3570
LmnI	GCTCC	7 cut(s)	378, 604, 824, 948, 1980, 2919, 3052
MaeI	CTAG	4 cut(s)	450, 1898, 2162, 3597
MaeII	ACGT	6 cut(s)	933, 954, 1270, 2099, 3217, 4048
MaeIII	GTNAC	5 cut(s)	155, 950, 2334, 3443, 3885
MbiI	CCGCTC	2 cut(s)	1597, 3307
MfeI	CAATTG	4 cut(s)	1721, 2304, 3452, 3606
MflI	RGATCY	5 cut(s)	766, 1864, 2572, 3115, 4265
MhlI	GDGCHC	9 cut(s)	43, 273, 1471, 1771, 2622, 3049, 3489, 3565, 4251
MlsI	TGGCCA	2 cut(s)	413, 4167
MluNI	TGGCCA	2 cut(s)	413, 4167
MlyI	GAGTC	7 cut(s)	132, 426, 860, 1358, 2135, 3041, 4451
Mox20I	TGGCCA	2 cut(s)	413, 4167
Mph1103I	ATGCAT	5 cut(s)	403, 1058, 3139, 3355, 3366
MroXI	GAANNNNTTC	6 cut(s)	218, 1558, 1797, 2790, 4074, 4503
MscI	TGGCCA	2 cut(s)	413, 4167
MslI	CAYNNNNRTG	4 cut(s)	1101, 1299, 2633, 3892
Msp20I	TGGCCA	2 cut(s)	413, 4167
MspA1I	CMGCKG	2 cut(s)	8, 2300
MspCI	CTTAAG	1 cut(s)	2012
MspI	CCGG	5 cut(s)	1533, 2093, 4027, 4181, 4374
MspR9I	CCNGG	7 cut(s)	376, 603, 1287, 1885, 2093, 2534, 3585
MunI	CAATTG	4 cut(s)	1721, 2304, 3452, 3606
Mva1269I	GAATGC	3 cut(s)	149, 1180, 1290
MvaI	CCWGG	6 cut(s)	376, 603, 1287, 1885, 2534, 3585
NciI	CCSGG	1 cut(s)	2093
NdeI	CATATG	1 cut(s)	3529
NmeAIII	GCCGAG	1 cut(s)	1132
NmuCI	GTSAC	1 cut(s)	3885
NsbI	TGCGCA	1 cut(s)	1313
NsiI	ATGCAT	5 cut(s)	403, 1058, 3139, 3355, 3366
NspI	RCATGY	3 cut(s)	2976, 3400, 3731
OliI	CACNNNNGTG	1 cut(s)	2633
PaeR7I	CTCGAG	5 cut(s)	36, 266, 868, 4010, 4244
PagI	TCATGA	2 cut(s)	2782, 4287
PciI	ACATGT	2 cut(s)	3396, 3727
PciSI	GCTCTTC	1 cut(s)	3570
PcsI	WCGNNNNNNNCGW	2 cut(s)	264, 2889
PctI	GAATGC	3 cut(s)	149, 1180, 1290
PdmI	GAANNNNTTC	6 cut(s)	218, 1558, 1797, 2790, 4074, 4503
Pfl23II	CGTACG	1 cut(s)	4082
PflFI	GACNNNGTC	1 cut(s)	1348
PflMI	CCANNNNNTGG	4 cut(s)	1643, 1723, 2876, 3260
PfoI	TCCNGGA	1 cut(s)	374
PinAI	ACCGGT	1 cut(s)	4026
Ple19I	CGATCG	1 cut(s)	63
PleI	GAGTC	7 cut(s)	131, 425, 860, 1357, 2134, 3040, 4451
PpsI	GAGTC	7 cut(s)	131, 425, 860, 1357, 2134, 3040, 4451
Ppu21I	YACGTR	2 cut(s)	934, 1271
PscI	ACATGT	2 cut(s)	3396, 3727
PshBI	ATTAAT	1 cut(s)	2073
PsiI	TTATAA	3 cut(s)	1332, 2955, 3200
Psp124BI	GAGCTC	2 cut(s)	273, 3049
Psp6I	CCWGG	6 cut(s)	374, 601, 1285, 1883, 2532, 3583
PspEI	GGTNACC	1 cut(s)	3443
PspGI	CCWGG	6 cut(s)	374, 601, 1285, 1883, 2532, 3583
PspLI	CGTACG	1 cut(s)	4082
PspPI	GGNCC	6 cut(s)	441, 500, 809, 2089, 2520, 2530
PspXI	VCTCGAGB	4 cut(s)	36, 266, 868, 4244
PsrI	GAACNNNNNNTAC	2 cut(s)	2745, 2777
PstNI	CAGNNNCTG	5 cut(s)	55, 638, 827, 1739, 2903
PsuI	RGATCY	5 cut(s)	766, 1864, 2572, 3115, 4265
PsyI	GACNNNGTC	1 cut(s)	1348
PvuI	CGATCG	1 cut(s)	63
PvuII	CAGCTG	1 cut(s)	2300
RsaI	GTAC	8 cut(s)	253, 896, 2502, 2754, 3179, 3401, 3465, 4084
RsaNI	GTAC	8 cut(s)	252, 895, 2501, 2753, 3178, 3400, 3464, 4083
RseI	CAYNNNNRTG	4 cut(s)	1101, 1299, 2633, 3892
SacI	GAGCTC	2 cut(s)	273, 3049
SapI	GCTCTTC	1 cut(s)	3570
Sau96I	GGNCC	6 cut(s)	441, 500, 809, 2089, 2520, 2530
ScaI	AGTACT	1 cut(s)	3465
SchI	GAGTC	7 cut(s)	132, 426, 860, 1358, 2135, 3041, 4451
ScrFI	CCNGG	7 cut(s)	376, 603, 1287, 1885, 2093, 2534, 3585
SduI	GDGCHC	9 cut(s)	43, 273, 1471, 1771, 2622, 3049, 3489, 3565, 4251
SfcI	CTRYAG	2 cut(s)	130, 2682
Sfr274I	CTCGAG	5 cut(s)	36, 266, 868, 4010, 4244
SinI	GGWCC	5 cut(s)	441, 500, 809, 2089, 2520
SlaI	CTCGAG	5 cut(s)	36, 266, 868, 4010, 4244
SmiMI	CAYNNNNRTG	4 cut(s)	1101, 1299, 2633, 3892
SnaBI	TACGTA	2 cut(s)	934, 1271
SsiI	CCGC	6 cut(s)	6, 182, 516, 1597, 2811, 3307
SspI	AATATT	3 cut(s)	1633, 2959, 3097
SspMI	CTAG	4 cut(s)	450, 1898, 2162, 3597
SstI	GAGCTC	2 cut(s)	273, 3049
StyD4I	CCNGG	7 cut(s)	374, 601, 1285, 1883, 2091, 2532, 3583
TaiI	ACGT	6 cut(s)	936, 957, 1273, 2102, 3220, 4051
TatI	WGTACW	4 cut(s)	894, 2752, 3177, 3463
TauI	GCSGC	2 cut(s)	518, 2813
TseFI	GTSAC	1 cut(s)	3885
Tsp45I	GTSAC	1 cut(s)	3885
TspGWI	ACGGA	5 cut(s)	513, 758, 1086, 4100, 4311
Tth111I	GACNNNGTC	1 cut(s)	1348
Van91I	CCANNNNNTGG	4 cut(s)	1643, 1723, 2876, 3260
Vha464I	CTTAAG	1 cut(s)	2012
VneI	GTGCAC	1 cut(s)	1767
VpaK11BI	GGWCC	5 cut(s)	441, 500, 809, 2089, 2520
VspI	ATTAAT	1 cut(s)	2073
XagI	CCTNNNNNAGG	1 cut(s)	2735
XapI	RAATTY	9 cut(s)	287, 999, 1392, 1458, 1912, 1922, 2045, 3104, 4369
XceI	RCATGY	3 cut(s)	2976, 3400, 3731
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3531, 4311
XhoI	CTCGAG	5 cut(s)	36, 266, 868, 4010, 4244
XmiI	GTMKAC	1 cut(s)	4051
XmnI	GAANNNNTTC	6 cut(s)	218, 1558, 1797, 2790, 4074, 4503
XspI	CTAG	4 cut(s)	450, 1898, 2162, 3597
ZrmI	AGTACT	1 cut(s)	3465
Zsp2I	ATGCAT	5 cut(s)	403, 1058, 3139, 3355, 3366

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.