MD05G1334200.v1.1

Minor allergen Alt a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001703
Physical Location & Seq
Forward (+)
45737172 .. 45740347
3176 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1334200.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGCCACCAAAATCTACATTGTGTACTATTCTACGTACGGACACGTTGCCAAGTTGGCACAAGAGATACTGAAAGGAGCTCAATCTGTCGAAGGAGTAGAGGCCAAACTATGGCAGGTACCAGAAACTCTGCCAGCAGAAGTTCTTGCAAAGATGGGTGCACCACCAAAGAGTGAAGTGCCTGTAATTTCACCTGAGCAACTTGCTGAGGCTGATGGGATAATCTTTGGTTTCCCTACCAGATTTGGAATGATGGCTGCACAATTTAAAGCATTTTTTGATTCAACTGGAAGTTTATGGCACAAAGGCGCACTTGCAGGCAAGCCTGCTGGACTTTTCTACTGCACTGGATCTCAAGGTGGTGGACAAGAGACTACCCCCTTGACAGCCATTACTCAGTTTACTCACCATGGAATGATCTTTGTCCCAATCGGATACACGGCCGGAGCTGGCATGTTCGAGATGGAGCAGGTCAAGGGTGGCAGCCCCTATGGTGCGGGAACTTTTGCTGGGGATGGCACAAGACAACCTTCTGATCTAGAACTAGAAATTGCTTGTCACCAGGGAAAGTACTTCGCTGGCATCGCGAAGAAGCTTAAGGGATCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01110 map00130 map01110
Pfam Domains
Protein Families
FMN_red

Protein Analysis

204

Amino Acids

21.46

Weight (kDa)

6.51

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Flavodoxin_2 PF02525 4 - 134 1.9e-07 Flavodoxin-like fold
Flavodoxin_1 PF00258 7 - 129 1.6e-08 Flavodoxin
FMN_red PF03358 15 - 146 5.7e-16 NADPH-dependent FMN reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 106, 460
Acc65I GGTACC 1 cut(s) 118
AccB1I GGYRCC 1 cut(s) 118
AccB7I CCANNNNNTGG 1 cut(s) 111
AccII CGCG 1 cut(s) 587
AciI CCGC 1 cut(s) 497
AclWI GGATC 2 cut(s) 358, 610
AcoI YGGCCR 2 cut(s) 3, 441
AfaI GTAC 4 cut(s) 26, 38, 120, 572
AfiI CCNNNNNNNGG 1 cut(s) 111
AflII CTTAAG 1 cut(s) 596
AflIII ACRYGT 1 cut(s) 43
AgsI TTSAA 1 cut(s) 285
AjnI CCWGG 1 cut(s) 561
AluBI AGCT 3 cut(s) 80, 449, 595
AluI AGCT 3 cut(s) 80, 449, 595
Alw21I GWGCWC 2 cut(s) 82, 163
Alw26I GTCTC 1 cut(s) 365
Alw44I GTGCAC 1 cut(s) 159
AlwI GGATC 2 cut(s) 358, 610
AoxI GGCC 3 cut(s) 3, 102, 441
ApaLI GTGCAC 1 cut(s) 159
ApeKI GCWGC 2 cut(s) 257, 483
Asp718I GGTACC 1 cut(s) 118
AspLEI GCGC 1 cut(s) 311
AsuHPI GGTGA 3 cut(s) 183, 398, 551
BaeGI GKGCMC 1 cut(s) 163
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 118
BanII GRGCYC 1 cut(s) 82
Bbv12I GWGCWC 2 cut(s) 82, 163
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 2 cut(s) 244, 495
BccI CCATC 5 cut(s) 148, 209, 247, 457, 509
BceAI ACGGC 1 cut(s) 456
BciT130I CCWGG 1 cut(s) 563
BciVI GTATCC 1 cut(s) 428
BcoDI GTCTC 1 cut(s) 365
BfaI CTAG 2 cut(s) 539, 545
BfrI CTTAAG 1 cut(s) 596
BfuAI ACCTGC 2 cut(s) 106, 460
BfuI GTATCC 1 cut(s) 428
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 2 cut(s) 258, 484
BlsI GCNGC 2 cut(s) 259, 485
BmcAI AGTACT 1 cut(s) 572
Bme1390I CCNGG 1 cut(s) 563
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 1 cut(s) 563
BmsI GCATC 1 cut(s) 591
Bpu10I CCTNAGC 2 cut(s) 195, 207
BpuEI CTTGAG 1 cut(s) 339
BsaAI YACGTR 1 cut(s) 36
BsaJI CCNNGG 2 cut(s) 409, 562
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse1I ACTGG 2 cut(s) 292, 352
BseBI CCWGG 1 cut(s) 563
BseDI CCNNGG 2 cut(s) 409, 562
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 3 cut(s) 186, 198, 410
BseNI ACTGG 2 cut(s) 292, 352
BseSI GKGCMC 1 cut(s) 163
BseX3I CGGCCG 1 cut(s) 441
BseXI GCAGC 2 cut(s) 244, 495
BseYI CCCAGC 1 cut(s) 509
BsgI GTGCAG 2 cut(s) 243, 328
Bsh1236I CGCG 1 cut(s) 587
Bsh1285I CGRYCG 1 cut(s) 444
BshFI GGCC 3 cut(s) 5, 104, 443
BshNI GGYRCC 1 cut(s) 118
BsiEI CGRYCG 1 cut(s) 444
BsiHKAI GWGCWC 2 cut(s) 82, 163
BsiSI CCGG 1 cut(s) 444
BsiWI CGTACG 1 cut(s) 36
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 365
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 3 cut(s) 5, 104, 443
Bsp1286I GDGCHC 2 cut(s) 82, 163
Bsp143I GATC 4 cut(s) 350, 417, 535, 602
Bsp19I CCATGG 1 cut(s) 409
Bsp68I TCGCGA 1 cut(s) 587
BspACI CCGC 1 cut(s) 497
BspANI GGCC 3 cut(s) 5, 104, 443
BspCNI CTCAG 3 cut(s) 187, 199, 409
BspFNI CGCG 1 cut(s) 587
BspLI GGNNCC 1 cut(s) 120
BspMI ACCTGC 2 cut(s) 106, 460
BspPI GGATC 2 cut(s) 358, 610
BspT107I GGYRCC 1 cut(s) 118
BspTI CTTAAG 1 cut(s) 596
BsrI ACTGG 2 cut(s) 292, 352
BssECI CCNNGG 2 cut(s) 409, 562
BssMI GATC 4 cut(s) 350, 417, 535, 602
BssT1I CCWWGG 1 cut(s) 409
Bst2UI CCWGG 1 cut(s) 563
BstAFI CTTAAG 1 cut(s) 596
BstBAI YACGTR 1 cut(s) 36
BstC8I GCNNGC 6 cut(s) 135, 319, 323, 327, 451, 580
BstDEI CTNAG 3 cut(s) 195, 207, 396
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 1 cut(s) 520
BstFNI CGCG 1 cut(s) 587
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 4 cut(s) 353, 420, 538, 605
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 4 cut(s) 350, 417, 535, 602
BstMCI CGRYCG 1 cut(s) 444
BstMWI GCNNNNNNNGC 2 cut(s) 56, 584
BstNI CCWGG 1 cut(s) 563
BstNSI RCATGY 1 cut(s) 457
BstSCI CCNGG 1 cut(s) 561
BstSLI GKGCMC 1 cut(s) 163
BstSNI TACGTA 1 cut(s) 36
BstUI CGCG 1 cut(s) 587
BstV1I GCAGC 2 cut(s) 244, 495
BstX2I RGATCY 2 cut(s) 350, 602
BstYI RGATCY 2 cut(s) 350, 602
BstZI CGGCCG 1 cut(s) 441
BsuI GTATCC 1 cut(s) 428
BsuRI GGCC 3 cut(s) 5, 104, 443
BtgI CCRYGG 1 cut(s) 409
BtgZI GCGATG 1 cut(s) 568
BtsCI GGATG 1 cut(s) 520
BtsIMutI CAGTG 1 cut(s) 345
BtuMI TCGCGA 1 cut(s) 587
BveI ACCTGC 2 cut(s) 106, 460
Cac8I GCNNGC 6 cut(s) 135, 319, 323, 327, 451, 580
CfoI GCGC 1 cut(s) 311
Csp6I GTAC 4 cut(s) 25, 37, 119, 571
CviAII CATG 2 cut(s) 410, 454
CviQI GTAC 4 cut(s) 25, 37, 119, 571
DdeI CTNAG 3 cut(s) 195, 207, 396
DpnI GATC 4 cut(s) 352, 419, 537, 604
DpnII GATC 4 cut(s) 350, 417, 535, 602
DraI TTTAAA 1 cut(s) 268
EaeI YGGCCR 2 cut(s) 3, 441
EagI CGGCCG 1 cut(s) 441
Ecl136II GAGCTC 1 cut(s) 80
EclXI CGGCCG 1 cut(s) 441
Eco105I TACGTA 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 409
Eco24I GRGCYC 1 cut(s) 82
Eco52I CGGCCG 1 cut(s) 441
Eco53kI GAGCTC 1 cut(s) 80
EcoICRI GAGCTC 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 561
EcoT14I CCWWGG 1 cut(s) 409
EcoT38I GRGCYC 1 cut(s) 82
ErhI CCWWGG 1 cut(s) 409
FaeI CATG 2 cut(s) 413, 457
FaiI YATR 5 cut(s) 112, 298, 411, 455, 492
FalI AAGNNNNNCTT 4 cut(s) 297, 329, 514, 546
FaqI GGGAC 1 cut(s) 410
FatI CATG 2 cut(s) 409, 453
FauI CCCGC 1 cut(s) 490
Fnu4HI GCNGC 2 cut(s) 258, 484
FokI GGATG 1 cut(s) 527
FriOI GRGCYC 1 cut(s) 82
Fsp4HI GCNGC 2 cut(s) 258, 484
FspBI CTAG 2 cut(s) 539, 545
GlaI GCGC 1 cut(s) 310
GluI GCNGC 2 cut(s) 258, 484
GsaI CCCAGC 1 cut(s) 513
HaeIII GGCC 3 cut(s) 5, 104, 443
HapII CCGG 1 cut(s) 444
HhaI GCGC 1 cut(s) 311
Hin1II CATG 2 cut(s) 413, 457
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 1 cut(s) 281
HpaII CCGG 1 cut(s) 444
HphI GGTGA 3 cut(s) 183, 398, 551
Hpy166II GTNNAC 4 cut(s) 25, 161, 365, 402
Hpy188I TCNGA 2 cut(s) 434, 535
Hpy188III TCNNGA 3 cut(s) 460, 539, 586
Hpy8I GTNNAC 4 cut(s) 25, 161, 365, 402
HpyAV CCTTC 2 cut(s) 86, 540
HpyCH4IV ACGT 2 cut(s) 35, 45
HpyCH4V TGCA 5 cut(s) 149, 161, 260, 317, 345
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 584
HpyF3I CTNAG 3 cut(s) 195, 207, 396
HpySE526I ACGT 2 cut(s) 35, 45
Hsp92II CATG 2 cut(s) 413, 457
HspAI GCGC 1 cut(s) 309
KpnI GGTACC 1 cut(s) 122
Kzo9I GATC 4 cut(s) 350, 417, 535, 602
LmnI GCTCC 3 cut(s) 77, 446, 466
Lsp1109I GCAGC 2 cut(s) 244, 495
LweI GCATC 1 cut(s) 591
MaeI CTAG 2 cut(s) 539, 545
MaeII ACGT 2 cut(s) 35, 45
MaeIII GTNAC 1 cut(s) 557
MalI GATC 4 cut(s) 352, 419, 537, 604
MboI GATC 4 cut(s) 350, 417, 535, 602
MboII GAAGA 1 cut(s) 601
MflI RGATCY 2 cut(s) 350, 602
MhlI GDGCHC 2 cut(s) 82, 163
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 186, 263, 549
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 2 cut(s) 94, 202
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 267, 597
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 596
MspI CCGG 1 cut(s) 444
MspR9I CCNGG 1 cut(s) 563
MvaI CCWGG 1 cut(s) 563
MvnI CGCG 1 cut(s) 587
MwoI GCNNNNNNNGC 2 cut(s) 56, 584
NcoI CCATGG 1 cut(s) 409
NdeII GATC 4 cut(s) 350, 417, 535, 602
NlaIII CATG 2 cut(s) 413, 457
NlaIV GGNNCC 1 cut(s) 120
NmuCI GTSAC 1 cut(s) 557
NruI TCGCGA 1 cut(s) 587
NspI RCATGY 1 cut(s) 457
PfeI GAWTC 1 cut(s) 281
Pfl23II CGTACG 1 cut(s) 36
PflMI CCANNNNNTGG 1 cut(s) 111
PkrI GCNGC 2 cut(s) 259, 485
Ppu21I YACGTR 1 cut(s) 36
Psp124BI GAGCTC 1 cut(s) 82
Psp6I CCWGG 1 cut(s) 561
PspFI CCCAGC 1 cut(s) 509
PspGI CCWGG 1 cut(s) 561
PspLI CGTACG 1 cut(s) 36
PspN4I GGNNCC 1 cut(s) 120
PsuI RGATCY 2 cut(s) 350, 602
RruI TCGCGA 1 cut(s) 587
RsaI GTAC 4 cut(s) 26, 38, 120, 572
RsaNI GTAC 4 cut(s) 25, 37, 119, 571
SacI GAGCTC 1 cut(s) 82
SaqAI TTAA 2 cut(s) 267, 597
SatI GCNGC 2 cut(s) 258, 484
Sau3AI GATC 4 cut(s) 350, 417, 535, 602
ScaI AGTACT 1 cut(s) 572
ScrFI CCNGG 1 cut(s) 563
SduI GDGCHC 2 cut(s) 82, 163
SfaNI GCATC 1 cut(s) 591
SmlI CTYRAG 2 cut(s) 354, 596
SmoI CTYRAG 2 cut(s) 354, 596
SnaBI TACGTA 1 cut(s) 36
Sse9I AATT 3 cut(s) 186, 263, 549
SsiI CCGC 1 cut(s) 497
SspMI CTAG 2 cut(s) 539, 545
SstI GAGCTC 1 cut(s) 82
StyD4I CCNGG 1 cut(s) 561
StyI CCWWGG 1 cut(s) 409
TaiI ACGT 2 cut(s) 38, 48
TaqI TCGA 2 cut(s) 90, 459
TasI AATT 3 cut(s) 186, 263, 549
TatI WGTACW 2 cut(s) 24, 570
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 2 cut(s) 267, 597
Tru9I TTAA 2 cut(s) 267, 597
TscAI CASTG 1 cut(s) 352
TseFI GTSAC 1 cut(s) 557
TseI GCWGC 2 cut(s) 257, 483
Tsp45I GTSAC 1 cut(s) 557
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 1 cut(s) 352
Van91I CCANNNNNTGG 1 cut(s) 111
Vha464I CTTAAG 1 cut(s) 596
VneI GTGCAC 1 cut(s) 159
XbaI TCTAGA 1 cut(s) 538
XceI RCATGY 1 cut(s) 457
XspI CTAG 2 cut(s) 539, 545
ZrmI AGTACT 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.