RLG00000014383

Minor allergen Alt a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001703
Physical Location & Seq
Forward (+)
52403657 .. 52406105
2449 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014383

Sequence Viewer

Length: 504 bp
ATGTCTCCATTGGCTCAATTAAATGGCGCTCTTCATCTTGATCGTCTCCAATCGGACAAGAAAACCATTCCTGGGGTGCCTGTGATAGTGCCTGAGAGACTGGTGGAGGCTGATGGGGTTTTGTTTGGGTTTCCTACCCGGTATGGGTCAATGGCTGCTCAGATGAAGGCGTTTTTCGACTCCACGGGGGAGCTTTGGAGGGAGCAGAAGCTTGCAGGGGTGCCTGCTGGGTTCTTTGTGAGTACTGGCACACAGGGTGGTGGACAGGAGACTAATGCTTGGACAGCCATTACTCAGTTAGCACACCATGGAATGATATATGTTCCAATTGGCTACTCCTTTGGTGCTGGAATGTTCCAAATGGACTCCCTTAGAGGAGGGTCTCCATATGGTTCTGGGGTTCTTTCTGGAGATGGCACAAGGGCACCAACTGAGACAGAGCTCGCTCTTGCAGAGCATCACGGAAAGTACATGGCTACTATTGTCAAGAAGCTTGTACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01110 map00130 map01110
Pfam Domains
Protein Families
FMN_red

Protein Analysis

168

Amino Acids

17.81

Weight (kDa)

6.58

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMN_red PF03358 32 - 112 2.2e-11 NADPH-dependent FMN reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 76, 220, 424
AdeI CACNNNGTG 1 cut(s) 257
AfaI GTAC 3 cut(s) 244, 470, 498
AfiI CCNNNNNNNGG 2 cut(s) 72, 144
AjnI CCWGG 1 cut(s) 70
AluBI AGCT 4 cut(s) 193, 211, 442, 493
AluI AGCT 4 cut(s) 193, 211, 442, 493
Alw21I GWGCWC 1 cut(s) 444
Alw26I GTCTC 6 cut(s) 9, 50, 91, 263, 387, 428
ApeKI GCWGC 1 cut(s) 155
AspLEI GCGC 1 cut(s) 29
AsuC2I CCSGG 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 427
BanI GGYRCC 3 cut(s) 76, 220, 424
BanII GRGCYC 1 cut(s) 444
Bbv12I GWGCWC 1 cut(s) 444
BbvI GCAGC 1 cut(s) 142
BccI CCATC 2 cut(s) 107, 407
BciT130I CCWGG 1 cut(s) 72
BcnI CCSGG 1 cut(s) 139
BcoDI GTCTC 6 cut(s) 9, 50, 91, 263, 387, 428
BfoI RGCGCY 1 cut(s) 30
BisI GCNGC 1 cut(s) 156
BlsI GCNGC 1 cut(s) 157
BmcAI AGTACT 1 cut(s) 244
Bme1390I CCNGG 2 cut(s) 72, 139
BmiI GGNNCC 3 cut(s) 78, 222, 426
BmrFI CCNGG 2 cut(s) 72, 139
BmsI GCATC 1 cut(s) 466
BpmI CTGGAG 1 cut(s) 429
BpuMI CCSGG 1 cut(s) 139
BsaI GGTCTC 1 cut(s) 387
BsaJI CCNNGG 3 cut(s) 71, 183, 307
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 144
Bse1I ACTGG 2 cut(s) 105, 250
BseBI CCWGG 1 cut(s) 72
BseDI CCNNGG 3 cut(s) 71, 183, 307
BseLI CCNNNNNNNGG 2 cut(s) 72, 144
BseMII CTCAG 4 cut(s) 84, 173, 308, 423
BseNI ACTGG 2 cut(s) 105, 250
BseRI GAGGAG 1 cut(s) 390
BseSI GKGCMC 1 cut(s) 427
BseXI GCAGC 1 cut(s) 142
BseYI CCCAGC 1 cut(s) 227
BshNI GGYRCC 3 cut(s) 76, 220, 424
BsiHKAI GWGCWC 1 cut(s) 444
BsiSI CCGG 1 cut(s) 139
BslI CCNNNNNNNGG 2 cut(s) 72, 144
BsmAI GTCTC 6 cut(s) 9, 50, 91, 263, 387, 428
BsmBI CGTCTC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 387
Bsp1286I GDGCHC 2 cut(s) 427, 444
Bsp1407I TGTACA 1 cut(s) 496
Bsp143I GATC 1 cut(s) 40
Bsp19I CCATGG 1 cut(s) 307
BspCNI CTCAG 4 cut(s) 85, 172, 307, 424
BspLI GGNNCC 3 cut(s) 78, 222, 426
BspQI GCTCTTC 1 cut(s) 36
BspT107I GGYRCC 3 cut(s) 76, 220, 424
BspTNI GGTCTC 1 cut(s) 387
BsrGI TGTACA 1 cut(s) 496
BsrI ACTGG 2 cut(s) 105, 250
BssECI CCNNGG 3 cut(s) 71, 183, 307
BssMI GATC 1 cut(s) 40
BssT1I CCWWGG 1 cut(s) 307
Bst2UI CCWGG 1 cut(s) 72
Bst6I CTCTTC 1 cut(s) 36
BstAUI TGTACA 1 cut(s) 496
BstC8I GCNNGC 3 cut(s) 213, 225, 444
BstDEI CTNAG 5 cut(s) 93, 159, 294, 371, 432
BstDSI CCRYGG 2 cut(s) 183, 307
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 1 cut(s) 43
BstMAI GTCTC 6 cut(s) 9, 50, 91, 263, 387, 428
BstMBI GATC 1 cut(s) 40
BstMWI GCNNNNNNNGC 1 cut(s) 284
BstNI CCWGG 1 cut(s) 72
BstSCI CCNGG 2 cut(s) 70, 137
BstSLI GKGCMC 1 cut(s) 427
BstV1I GCAGC 1 cut(s) 142
BtgI CCRYGG 2 cut(s) 183, 307
Cac8I GCNNGC 3 cut(s) 213, 225, 444
CfoI GCGC 1 cut(s) 29
Csp6I GTAC 3 cut(s) 243, 469, 497
CviAII CATG 2 cut(s) 308, 472
CviQI GTAC 3 cut(s) 243, 469, 497
DdeI CTNAG 5 cut(s) 93, 159, 294, 371, 432
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
DraIII CACNNNGTG 1 cut(s) 257
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 442
Eco130I CCWWGG 1 cut(s) 307
Eco24I GRGCYC 1 cut(s) 444
Eco31I GGTCTC 1 cut(s) 387
Eco53kI GAGCTC 1 cut(s) 442
EcoICRI GAGCTC 1 cut(s) 442
EcoRII CCWGG 1 cut(s) 70
EcoT14I CCWWGG 1 cut(s) 307
EcoT38I GRGCYC 1 cut(s) 444
ErhI CCWWGG 1 cut(s) 307
Esp3I CGTCTC 1 cut(s) 50
FaeI CATG 2 cut(s) 311, 475
FaiI YATR 7 cut(s) 144, 309, 319, 321, 388, 390, 473
FatI CATG 2 cut(s) 307, 471
FauNDI CATATG 1 cut(s) 388
Fnu4HI GCNGC 1 cut(s) 156
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 1 cut(s) 156
GlaI GCGC 1 cut(s) 28
GluI GCNGC 1 cut(s) 156
GsaI CCCAGC 1 cut(s) 231
GsuI CTGGAG 1 cut(s) 429
HaeII RGCGCY 1 cut(s) 30
HapII CCGG 1 cut(s) 139
HhaI GCGC 1 cut(s) 29
Hin1II CATG 2 cut(s) 311, 475
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HindIII AAGCTT 2 cut(s) 209, 491
HinfI GANTC 2 cut(s) 179, 365
HpaII CCGG 1 cut(s) 139
Hpy166II GTNNAC 1 cut(s) 263
Hpy188I TCNGA 2 cut(s) 55, 162
Hpy188III TCNNGA 3 cut(s) 38, 408, 487
Hpy8I GTNNAC 1 cut(s) 263
HpyAV CCTTC 1 cut(s) 160
HpyCH4V TGCA 2 cut(s) 215, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 284
HpyF3I CTNAG 5 cut(s) 93, 159, 294, 371, 432
Hsp92II CATG 2 cut(s) 311, 475
HspAI GCGC 1 cut(s) 27
Kzo9I GATC 1 cut(s) 40
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 2 cut(s) 190, 202
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 1 cut(s) 466
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MboII GAAGA 1 cut(s) 23
MfeI CAATTG 1 cut(s) 327
MhlI GDGCHC 2 cut(s) 427, 444
MluCI AATT 2 cut(s) 17, 327
MlyI GAGTC 2 cut(s) 173, 359
MnlI CCTC 4 cut(s) 100, 192, 368, 371
MseI TTAA 1 cut(s) 20
MspI CCGG 1 cut(s) 139
MspR9I CCNGG 2 cut(s) 72, 139
MunI CAATTG 1 cut(s) 327
MvaI CCWGG 1 cut(s) 72
MwoI GCNNNNNNNGC 1 cut(s) 284
NciI CCSGG 1 cut(s) 139
NcoI CCATGG 1 cut(s) 307
NdeI CATATG 1 cut(s) 388
NdeII GATC 1 cut(s) 40
NlaIII CATG 2 cut(s) 311, 475
NlaIV GGNNCC 3 cut(s) 78, 222, 426
PciSI GCTCTTC 1 cut(s) 36
PkrI GCNGC 1 cut(s) 157
PleI GAGTC 2 cut(s) 173, 359
PpsI GAGTC 2 cut(s) 173, 359
Psp124BI GAGCTC 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 70
PspFI CCCAGC 1 cut(s) 227
PspGI CCWGG 1 cut(s) 70
PspN4I GGNNCC 3 cut(s) 78, 222, 426
RsaI GTAC 3 cut(s) 244, 470, 498
RsaNI GTAC 3 cut(s) 243, 469, 497
SacI GAGCTC 1 cut(s) 444
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 1 cut(s) 156
Sau3AI GATC 1 cut(s) 40
ScaI AGTACT 1 cut(s) 244
SchI GAGTC 2 cut(s) 173, 359
ScrFI CCNGG 2 cut(s) 72, 139
SduI GDGCHC 2 cut(s) 427, 444
SetI ASST 4 cut(s) 195, 213, 444, 495
SfaNI GCATC 1 cut(s) 466
Sse9I AATT 2 cut(s) 17, 327
SstI GAGCTC 1 cut(s) 444
StyD4I CCNGG 2 cut(s) 70, 137
StyI CCWWGG 1 cut(s) 307
TaqI TCGA 1 cut(s) 177
TasI AATT 2 cut(s) 17, 327
TatI WGTACW 3 cut(s) 242, 468, 496
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TseI GCWGC 1 cut(s) 155
TspDTI ATGAA 2 cut(s) 23, 179
TspGWI ACGGA 1 cut(s) 477
ZrmI AGTACT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.