Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001276

Physical Location & Seq

Genomic Coordinates Reverse (-)

27579397 .. 27583297

Gene Structure 3901 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1131900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3081 bp

ATGGGGTTATTTTCTCATGGAAATCTCTTTGCATTTCATCTGAGCAATCGAATTGGTATTGCAAAAGCAAGACTTGCGATTCATATCAAATATTGCACCACCACCTCGCAGCAATCAGAGACGTTCAAACAGGACGATGACGAGACAGTGAGAGAAATCTCCACCATCCTCAGAAACCACAGCGACTGGCACTTGGTTCTCAACTCCTCGCACCTCCCCAGGAAGCTGAACCCACATGTGGTCCGAGCCGTTCTGCAGCAGAACCACCAGGTTGGTGACCCAAAGCGCCTCCTCAGTTTCTTCCTATGGACCGATACCCATCTGGGTCTTCCCCAAAATTTGCATTCCTTTTCGATTCTTGCTGTTGTTCTGTGCAATTCCAAGATGTTTGAGCAGGCTAATGCTGTGTTAGATAGAATGGTTAAGACCCGAAAACCGGTTTTTGAAGTTTTGGATTCTGTTGTTAGTTGTTTTAGAGATGGTGAGTGTGATGGATCTGATAAGATTGTTTTTGAGATTCTGATTAGAGCTTTTATGGCGGCGGGGCGGTTGAATGAGGCTGCTGATGTTTTTCTGGGACTTAGGAAAGTTGGGATTTTGCCAAGATTGGATTGCTGCAATTTTCTGTTGAATGATTTGTTGAAGTGTAATAAAATGGAGTTGTTTTGGAAGGTGTATGATGGGATGTTAGGGGCGAAAATGAAACCCGATTTTTACACTTATTACAATGTGATCCATGCCCATTGTAGGGCAGGGAATGCCGGGCAGGGAAAGAGGTTTCTGTTTGAGATGGAGGAGAAGGGTGGTAATCCAGATTTAAGTACCTATAATGTGGTGATTGGTGGTTTGTGTAGAGCTGGGGATGTCGATGAAGCCTTAGCGGTAAAGAAGTCTATGGTTGAGAAGGGATTGGTCCCGGATAGGTATACTTATTCGGCACTTGTTGATGGGTTATGCAGACAGAAGAGATCAACAGAAGCAAAATTGATGTTGAAATACATGTATGATGAAGGTTTGAGTCCTGACAACACCTGCTACGCTGCCTTGATTGATGGGTTGATGAAAGAAGGTTACTTGGAAGAGGCTCTGAGGATCAAAGATGAGACAATTGATCGTGGCTTTAAGTTGTGCGATTCCACATGTAATGCAATTTTTTCTGGGATGTCTAAAATTGGTAAGATGGAGAAGGCAGAAGCTCTCTTGAATGAGATGAATGTTATGGGCACTAGACCTAATGCCCAGACATACAAGTTCTTAATTGACGGGTATTGTCGAGAGCAAAATATGGTCAAGGCTTGTGAGTTACTTAACGAGATGAAGAAGAGGAACTTTGCACCCAATGTGTACACTTATGGAGCTATAATCAATGGCCTAAGCCGGTGTGGAGATATGCAAGGGGCTAATCAACTTTTGAAGGAAATGACTAGCAGAGGTTTGAAGCCAGGTGCTGTTGTCTACACAACTGTAATTAGAGGTCACGTCCAGGAGGGAAAATTTGGAGAGGCAATCAAAGTACTAAAAGGAATGACGGAAAAGGGGGTCATGCCTGATGCCTTTTGCTACAATTCTCTTATAATTGGCCTCTGTAAAGCCAGGAAAATGGACGAAGCCAGGACTTACTTCGTGGAAATGGTTGAGAGAGGATTAAAGCCTAATGTGTATACTTACGGGGCTTTTATTCATGGGTACTGTAAGGAGGGGAAAATGCAACTTGCGAATACGTATTTCCAGGAGATGTTGAGTTGCGGTATAGCTCCTAATGATGTTATCTATACAGCCCTGATTGAAGGGCACTGCAAAGAGGGTAACTTAACGGAAGTATATTCTACATTTAGGTGTATGCTTGGACGAGGGGTGCTCCCAGATATCAAAACTTATAGTGTCATTATTCATGGTCTCTCAAAGAATGGAAAAATTCAAGAAGCAATGGGGATTTTCTCCGAGCTCCTTGGCAAGGATCTGGTTCCAGATGTTTTTACTTACAGCTCTCTCGTATCTGGCCTTTGTAAGCAGGGAAATGTGGATAAGGCTTTCGATCTTCTTGAACAGATGTGCCAGAGAGGCGTTGATCCAAACATTGTTACTTATAACGCCTTGATTAATGGATTGTGTAAGTCAGGTGACACTGATAGTGCCAGAGAACTGTTTGATGGAATCTCCAGAAAGGGTTTGTCTCCTAATGCTGTGACTTATGCTACAATGATGGACGGATATAGCAAATCCGGAAATATAACCGAGGCATTTCAGTTATTAGATGAGATGCTTCTACGTGGGATCCCTACAGATAGCTTCATCTACTGCATCCTCATTGATGGGTGCTGCAAGGCAGGTGACCTGGAGAAGGCCCTGTCTTTATTTCGGGGTATGGTGGGGAAGGGCATTGCTGCCACTTCTCCTTTCAATGCATTGATTGATGGCTTCTGCAAATTGGGGAGGGTGATTGAAGCTAAACAGTTGTTGGAAGATATGGTGGATAAGCATGTGACACCAGATCAAGTCACGTACACGATTCTGATTGTTTCCCTCTGCAAAGAGGGATTGATGAGAGAATCAGAACAGCTCTTTCTGGAAATGCAGAAAAGGAATATGACGCCAGATGTTTTAATGTATGCTTCACTTTTACACGGATACAATAGCACGGGAAGCAGATTTAAGATGTTTGCTCTCTTTGAGGACATGGTGACGAGGGGACTCAAGCCTAATGAAGTTACATATTGTATGATGGTTGATGCTTATTGCAAAGAAGGTGATTTGGTAAAATGTTTAAGGTTGGTGGATGAAACTTTGGTCAATGGTGCAATCTCGAACAGTGTAGTGGTTGATGCACTGACAGGTACACTATTCCAAGGGGAAGAATTTGCTGAGACAATGAAGGCACTTGATGAAATGGGAGAACAAGGATTTGTGCTCAGTCTTGCTACATGCAGCACATTAGTTCACGGATTTTACAAATTAGGGAATGGGGAGAAAGCAGCAAGGATTTTTGAGAGCATGCTTAGGTTTGGATGGGTTTCACAGTCCACCAGTTTAGATGACTTAATTCATGAGGACCAAAGTGAGGCTAGCTCCGGAATATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00260 ko00261 ko00270 ko00300 ko01100 ko01110 ko01120 ko01130 ko01210 ko01230 map00260 map00261 map00270 map00300 map01100 map01110 map01120 map01130 map01210 map01230

Pfam Domains

Protein Families

Mem_trans PPR PPR_1 PPR_2 PPR_3 PPR_long

Protein Analysis

1027

Amino Acids

114.27

Weight (kDa)

6.19

Isoelectric Point (pI)

26.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	205 - 250	2.9e-06	PPR repeat family
PPR_long	PF17177	219 - 315	2.4e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	243 - 285	2e-07	PPR repeat family
PPR_1	PF12854	267 - 300	7.9e-10	PPR repeat
PPR_2	PF13041	271 - 320	3.1e-16	PPR repeat family
PPR	PF01535	274 - 304	3e-07	PPR repeat
PPR_1	PF12854	302 - 334	3.8e-07	PPR repeat
PPR_2	PF13041	314 - 355	7.1e-06	PPR repeat family
PPR_2	PF13041	379 - 425	7.7e-13	PPR repeat family
PPR_3	PF13812	400 - 456	7.9e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	408 - 440	1.7e-08	PPR repeat
PPR	PF01535	415 - 444	5.2e-06	PPR repeat
PPR_2	PF13041	416 - 459	1.7e-14	PPR repeat family
PPR_3	PF13812	435 - 482	7.3e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	443 - 475	6e-07	PPR repeat
PPR_3	PF13812	470 - 529	3.6e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	481 - 530	2.3e-13	PPR repeat family
PPR	PF01535	484 - 514	9.5e-06	PPR repeat
PPR_long	PF17177	502 - 609	1.9e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	512 - 545	1.5e-08	PPR repeat
PPR_2	PF13041	516 - 564	4.8e-15	PPR repeat family
PPR	PF01535	520 - 549	1.5e-07	PPR repeat
PPR_3	PF13812	543 - 596	8.5e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	547 - 579	1.8e-08	PPR repeat
PPR	PF01535	554 - 584	3e-06	PPR repeat
PPR_long	PF17177	574 - 683	5.2e-08	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	586 - 635	1.1e-12	PPR repeat family
PPR_long	PF17177	605 - 707	9.7e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	617 - 648	6.6e-06	PPR repeat
PPR_2	PF13041	629 - 670	2.4e-10	PPR repeat family
PPR_3	PF13812	645 - 701	6.8e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	652 - 684	2.7e-14	PPR repeat
PPR	PF01535	659 - 689	3.7e-08	PPR repeat
PPR_2	PF13041	664 - 705	8e-16	PPR repeat family
PPR_long	PF17177	673 - 758	1.4e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	680 - 738	4.7e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	687 - 717	3.1e-12	PPR repeat
PPR_2	PF13041	691 - 740	1.4e-16	PPR repeat family
PPR	PF01535	694 - 724	1.6e-07	PPR repeat
PPR_1	PF12854	722 - 754	2.3e-10	PPR repeat
PPR_2	PF13041	726 - 775	3.4e-15	PPR repeat family
PPR	PF01535	729 - 759	1.8e-07	PPR repeat
PPR_1	PF12854	757 - 790	1.4e-08	PPR repeat
PPR_2	PF13041	762 - 796	4.6e-06	PPR repeat family
PPR	PF01535	765 - 794	2.4e-08	PPR repeat
PPR_long	PF17177	769 - 926	1e-08	Pentacotripeptide-repeat region of PRORP
PPR	PF01535	800 - 826	1.8e-06	PPR repeat
PPR_1	PF12854	800 - 824	1e-06	PPR repeat
PPR_2	PF13041	800 - 844	1.2e-13	PPR repeat family
PPR_1	PF12854	827 - 858	1.8e-08	PPR repeat
PPR	PF01535	833 - 862	6.6e-07	PPR repeat
PPR_2	PF13041	838 - 877	9.1e-06	PPR repeat family
PPR_3	PF13812	854 - 914	2.9e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	865 - 914	2e-10	PPR repeat family
PPR_1	PF12854	896 - 927	1.4e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001276)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61990
fragaria_vesca	FvH4_5g09340 FvH4_5g09340 FvH4_5g09340 FvH4_5g09340
malus_domestica	MD06G1131900.v1.1 MD14G1148300.v1.1
prunus_persica	Prupe.5G142900_v2.0.a1 Prupe.5G142900_v2.0.a1
pyrus_communis	pycom06g12350 pycom14g12300
rosa_chinensis	RchiOBHm_Chr1g0325241 RchiOBHm_Chr1g0325261 RchiOBHm_Chr2g0162681 RchiOBHm_Chr7g0188811 RchiOBHm_Chr7g0229441
rosa_laevigata	RLG00000004657 RLG00000028121 RLG00000028122 RLG00000031426 RLG00000035825
rosa_multiflora	Rmu_sc0001621.1_g000013 Rmu_sc0001918.1_g000006 Rmu_sc0004279.1_g000018 Rmu_sc0034360.1_g000001 Rmu_sc0034361.1_g000001
rosa_roxburghii	Rroxscaffold_3G00266000 Rroxscaffold_5G00381140
rosa_rugosa	Rorug02G0360500 Rorug05G0369000 Rorug06G0494100 Rorug07G0156300
rosa_samantha	Rh1BG033100 Rh2CG348200 Rh2CG348300 Rh4AG001800 Rh4AG163800 Rh4BG001600 Rh4BG400000 Rh4BG400100 Rh4CG415000 Rh4CG415100 Rh6DG332400 Rh6DG332500 Rh7AG098900 Rh7AG408500 Rh7BG100600 Rh7CG102100 Rh7CG477400 Rh7DG100700 Rh7DG406300 Rh7DG406400 Rh7DG446700
rosa_wichuraiana	Rw0G022150 Rw7G008520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1574, 2088
AarI	CACCTGC	2 cut(s)	1040, 2318
Acc36I	ACCTGC	2 cut(s)	1040, 2318
AccI	GTMKAC	3 cut(s)	926, 1455, 1661
AccIII	TCCGGA	2 cut(s)	2222, 3068
AciI	CCGC	5 cut(s)	539, 542, 547, 881, 1746
AclWI	GGATC	7 cut(s)	502, 727, 1100, 1965, 2063, 2269, 2282
AcsI	RAATTY	4 cut(s)	337, 1493, 1914, 2855
AcyI	GRCGYC	1 cut(s)	2591
AfaI	GTAC	6 cut(s)	823, 1346, 1515, 1688, 2504, 2836
AfiI	CCNNNNNNNGG	7 cut(s)	238, 436, 747, 748, 1954, 2341, 3058
AflIII	ACRYGT	3 cut(s)	235, 999, 1139
AgeI	ACCGGT	1 cut(s)	436
AjiI	CACGTC	1 cut(s)	1480
AjnI	CCWGG	8 cut(s)	218, 267, 1441, 1482, 1592, 1610, 1728, 2334
Alw21I	GWGCWC	3 cut(s)	1860, 1947, 2910
Alw26I	GTCTC	6 cut(s)	113, 137, 1097, 1901, 2178, 2858
AlwI	GGATC	7 cut(s)	502, 727, 1100, 1965, 2063, 2269, 2282
AlwNI	CAGNNNCTG	2 cut(s)	186, 1448
Aor13HI	TCCGGA	2 cut(s)	2222, 3068
AoxI	GGCC	4 cut(s)	1369, 1579, 1999, 2343
ApeKI	GCWGC	9 cut(s)	109, 256, 560, 615, 1040, 2319, 2384, 2925, 2972
ApoI	RAATTY	4 cut(s)	337, 1493, 1914, 2855
AseI	ATTAAT	1 cut(s)	2100
AsiGI	ACCGGT	1 cut(s)	436
AspLEI	GCGC	1 cut(s)	288
AspS9I	GGNCC	5 cut(s)	241, 309, 913, 2344, 3049
AsuC2I	CCSGG	2 cut(s)	763, 917
AsuHPI	GGTGA	8 cut(s)	287, 494, 847, 2132, 2342, 2449, 2692, 2759
AsuNHI	GCTAGC	1 cut(s)	3062
AvaII	GGWCC	4 cut(s)	241, 309, 913, 3049
BaeGI	GKGCMC	2 cut(s)	1226, 1794
BamHI	GGATCC	1 cut(s)	2274
BanII	GRGCYC	1 cut(s)	1947
BbsI	GAAGAC	1 cut(s)	320
Bbv12I	GWGCWC	3 cut(s)	1860, 1947, 2910
BbvI	GCAGC	9 cut(s)	121, 268, 547, 602, 1027, 2306, 2371, 2937, 2984
BceAI	ACGGC	1 cut(s)	233
BciT130I	CCWGG	8 cut(s)	220, 269, 1443, 1484, 1594, 1612, 1730, 2336
BciVI	GTATCC	1 cut(s)	2621
BcnI	CCSGG	2 cut(s)	763, 917
BcoDI	GTCTC	6 cut(s)	113, 137, 1097, 1901, 2178, 2858
BfaI	CTAG	3 cut(s)	1227, 1425, 3063
BfmI	CTRYAG	2 cut(s)	254, 2280
BfoI	RGCGCY	1 cut(s)	289
BfuAI	ACCTGC	2 cut(s)	1040, 2318
BfuI	GTATCC	1 cut(s)	2621
BglI	GCCNNNNNGGC	1 cut(s)	2061
BmcAI	AGTACT	1 cut(s)	1515
Bme18I	GGWCC	4 cut(s)	241, 309, 913, 3049
BmgBI	CACGTC	1 cut(s)	1480
BmgT120I	GGNCC	5 cut(s)	241, 309, 913, 2344, 3049
BmiI	GGNNCC	3 cut(s)	915, 1965, 2276
BmsI	GCATC	5 cut(s)	1540, 2250, 2310, 2719, 2812
BmtI	GCTAGC	1 cut(s)	3066
BpiI	GAAGAC	1 cut(s)	320
BplI	GAGNNNNNCTC	3 cut(s)	1842, 1874, 3050
BpmI	CTGGAG	2 cut(s)	2143, 2357
Bpu10I	CCTNAGC	3 cut(s)	877, 1373, 2996
BpuEI	CTTGAG	1 cut(s)	2678
BpuMI	CCSGG	2 cut(s)	763, 917
BsaAI	YACGTR	3 cut(s)	1722, 2270, 2502
BsaBI	GATNNNNATC	2 cut(s)	83, 318
BsaHI	GRCGYC	1 cut(s)	2591
BsaI	GGTCTC	1 cut(s)	1901
BsaJI	CCNNGG	4 cut(s)	218, 1948, 2235, 2845
BsaWI	WCCGGW	3 cut(s)	436, 2222, 3068
Bsc4I	CCNNNNNNNGG	7 cut(s)	238, 436, 747, 748, 1954, 2341, 3058
Bse118I	RCCGGY	2 cut(s)	436, 1377
Bse1I	ACTGG	2 cut(s)	191, 3024
Bse3DI	GCAATG	2 cut(s)	1932, 2379
Bse8I	GATNNNNATC	2 cut(s)	83, 318
BseAI	TCCGGA	2 cut(s)	2222, 3068
BseBI	CCWGG	8 cut(s)	220, 269, 1443, 1484, 1594, 1612, 1730, 2336
BseDI	CCNNGG	4 cut(s)	218, 1948, 2235, 2845
BseGI	GGATG	7 cut(s)	165, 690, 868, 1167, 2301, 2782, 3011
BseJI	GATNNNNATC	2 cut(s)	83, 318
BseLI	CCNNNNNNNGG	7 cut(s)	238, 436, 747, 748, 1954, 2341, 3058
BseMI	GCAATG	2 cut(s)	1932, 2379
BseMII	CTCAG	6 cut(s)	32, 184, 307, 1079, 2853, 2923
BseNI	ACTGG	2 cut(s)	191, 3024
BseRI	GAGGAG	3 cut(s)	196, 281, 809
BseSI	GKGCMC	2 cut(s)	1226, 1794
BseXI	GCAGC	9 cut(s)	121, 268, 547, 602, 1027, 2306, 2371, 2937, 2984
BseYI	CCCAGC	1 cut(s)	857
BshFI	GGCC	4 cut(s)	1371, 1581, 2001, 2345
BshTI	ACCGGT	1 cut(s)	436
BsiHKAI	GWGCWC	3 cut(s)	1860, 1947, 2910
BsiSI	CCGG	6 cut(s)	437, 762, 917, 1378, 2223, 3069
BslFI	GGGAC	3 cut(s)	591, 899, 2703
BslI	CCNNNNNNNGG	7 cut(s)	238, 436, 747, 748, 1954, 2341, 3058
BsmAI	GTCTC	6 cut(s)	113, 137, 1097, 1901, 2178, 2858
BsmBI	CGTCTC	1 cut(s)	113
BsmFI	GGGAC	3 cut(s)	591, 899, 2703
BsmI	GAATGC	2 cut(s)	343, 763
BsnI	GGCC	4 cut(s)	1371, 1581, 2001, 2345
Bso31I	GGTCTC	1 cut(s)	1901
Bsp1286I	GDGCHC	5 cut(s)	1226, 1794, 1860, 1947, 2910
Bsp13I	TCCGGA	2 cut(s)	2222, 3068
Bsp1407I	TGTACA	1 cut(s)	1344
BspACI	CCGC	5 cut(s)	539, 542, 547, 881, 1746
BspANI	GGCC	4 cut(s)	1371, 1581, 2001, 2345
BspCNI	CTCAG	6 cut(s)	33, 183, 306, 1080, 2854, 2922
BspEI	TCCGGA	2 cut(s)	2222, 3068
BspHI	TCATGA	1 cut(s)	3043
BspLI	GGNNCC	3 cut(s)	915, 1965, 2276
BspMAI	CTGCAG	1 cut(s)	258
BspMI	ACCTGC	2 cut(s)	1040, 2318
BspOI	GCTAGC	1 cut(s)	3066
BspPI	GGATC	7 cut(s)	502, 727, 1100, 1965, 2063, 2269, 2282
BspTNI	GGTCTC	1 cut(s)	1901
BsrDI	GCAATG	2 cut(s)	1932, 2379
BsrFI	RCCGGY	2 cut(s)	436, 1377
BsrGI	TGTACA	1 cut(s)	1344
BsrI	ACTGG	2 cut(s)	191, 3024
BssAI	RCCGGY	2 cut(s)	436, 1377
BssECI	CCNNGG	4 cut(s)	218, 1948, 2235, 2845
BssNAI	GTATAC	2 cut(s)	927, 1662
BssNI	GRCGYC	1 cut(s)	2591
BssT1I	CCWWGG	2 cut(s)	1948, 2845
Bst1107I	GTATAC	2 cut(s)	927, 1662
Bst2UI	CCWGG	8 cut(s)	220, 269, 1443, 1484, 1594, 1612, 1730, 2336
Bst4CI	ACNGT	7 cut(s)	148, 1465, 1691, 2145, 2454, 2810, 3018
Bst6I	CTCTTC	3 cut(s)	959, 1074, 1316
BstACI	GRCGYC	1 cut(s)	2591
BstAPI	GCANNNNNTGC	2 cut(s)	74, 758
BstAUI	TGTACA	1 cut(s)	1344
BstBAI	YACGTR	3 cut(s)	1722, 2270, 2502
BstC8I	GCNNGC	3 cut(s)	396, 2993, 3064
BstEII	GGTNACC	2 cut(s)	275, 2330
BstENI	CCTNNNNNAGG	2 cut(s)	1952, 2339
BstF5I	GGATG	7 cut(s)	165, 690, 868, 1167, 2301, 2782, 3011
BstH2I	RGCGCY	1 cut(s)	289
BstHHI	GCGC	1 cut(s)	288
BstMAI	GTCTC	6 cut(s)	113, 137, 1097, 1901, 2178, 2858
BstMWI	GCNNNNNNNGC	5 cut(s)	74, 536, 758, 2061, 2643
BstNI	CCWGG	8 cut(s)	220, 269, 1443, 1484, 1594, 1612, 1730, 2336
BstNSI	RCATGY	6 cut(s)	239, 1003, 1143, 2483, 2925, 2995
BstPI	GGTNACC	2 cut(s)	275, 2330
BstSFI	CTRYAG	2 cut(s)	254, 2280
BstSLI	GKGCMC	2 cut(s)	1226, 1794
BstSNI	TACGTA	1 cut(s)	1722
BstV1I	GCAGC	9 cut(s)	121, 268, 547, 602, 1027, 2306, 2371, 2937, 2984
BstV2I	GAAGAC	1 cut(s)	320
BstX2I	RGATCY	3 cut(s)	494, 1957, 2274
BstXI	CCANNNNNNTGG	2 cut(s)	272, 1600
BstYI	RGATCY	3 cut(s)	494, 1957, 2274
BstZ17I	GTATAC	2 cut(s)	927, 1662
BsuI	GTATCC	1 cut(s)	2621
BsuRI	GGCC	4 cut(s)	1371, 1581, 2001, 2345
BtrI	CACGTC	1 cut(s)	1480
BtsCI	GGATG	7 cut(s)	165, 690, 868, 1167, 2301, 2782, 3011
BtsI	GCAGTG	1 cut(s)	1792
BtsIMutI	CAGTG	5 cut(s)	153, 1792, 2124, 2815, 2825
BveI	ACCTGC	2 cut(s)	1040, 2318
Cac8I	GCNNGC	3 cut(s)	396, 2993, 3064
CaiI	CAGNNNCTG	2 cut(s)	186, 1448
CciI	TCATGA	1 cut(s)	3043
CfoI	GCGC	1 cut(s)	288
Cfr10I	RCCGGY	2 cut(s)	436, 1377
Cfr13I	GGNCC	5 cut(s)	241, 309, 913, 2344, 3049
CseI	GACGC	1 cut(s)	2599
CsiI	ACCWGGT	1 cut(s)	267
Csp6I	GTAC	6 cut(s)	822, 1345, 1514, 1687, 2503, 2835
CspAI	ACCGGT	1 cut(s)	436
CviQI	GTAC	6 cut(s)	822, 1345, 1514, 1687, 2503, 2835
Eam1104I	CTCTTC	3 cut(s)	959, 1074, 1316
EarI	CTCTTC	3 cut(s)	959, 1074, 1316
Ecl136II	GAGCTC	1 cut(s)	1945
Eco105I	TACGTA	1 cut(s)	1722
Eco130I	CCWWGG	2 cut(s)	1948, 2845
Eco24I	GRGCYC	1 cut(s)	1947
Eco31I	GGTCTC	1 cut(s)	1901
Eco32I	GATATC	1 cut(s)	1867
Eco47I	GGWCC	4 cut(s)	241, 309, 913, 3049
Eco53kI	GAGCTC	1 cut(s)	1945
Eco91I	GGTNACC	2 cut(s)	275, 2330
EcoICRI	GAGCTC	1 cut(s)	1945
EcoNI	CCTNNNNNAGG	2 cut(s)	1952, 2339
EcoO109I	RGGNCCY	1 cut(s)	2344
EcoO65I	GGTNACC	2 cut(s)	275, 2330
EcoRII	CCWGG	8 cut(s)	218, 267, 1441, 1482, 1592, 1610, 1728, 2334
EcoRV	GATATC	1 cut(s)	1867
EcoT14I	CCWWGG	2 cut(s)	1948, 2845
EcoT22I	ATGCAT	1 cut(s)	2407
EcoT38I	GRGCYC	1 cut(s)	1947
ErhI	CCWWGG	2 cut(s)	1948, 2845
Esp3I	CGTCTC	1 cut(s)	113
FalI	AAGNNNNNCTT	4 cut(s)	57, 89, 1313, 1345
FaqI	GGGAC	3 cut(s)	591, 899, 2703
FauI	CCCGC	1 cut(s)	535
FblI	GTMKAC	3 cut(s)	926, 1455, 1661
FokI	GGATG	7 cut(s)	152, 697, 875, 1174, 2288, 2789, 3018
FriOI	GRGCYC	1 cut(s)	1947
FspBI	CTAG	3 cut(s)	1227, 1425, 3063
GlaI	GCGC	1 cut(s)	287
GsaI	CCCAGC	1 cut(s)	861
GsuI	CTGGAG	2 cut(s)	2143, 2357
HaeII	RGCGCY	1 cut(s)	289
HaeIII	GGCC	4 cut(s)	1371, 1581, 2001, 2345
HapII	CCGG	6 cut(s)	437, 762, 917, 1378, 2223, 3069
HgaI	GACGC	1 cut(s)	2599
HhaI	GCGC	1 cut(s)	288
Hin1I	GRCGYC	1 cut(s)	2591
Hin6I	GCGC	1 cut(s)	286
HinP1I	GCGC	1 cut(s)	286
HpaII	CCGG	6 cut(s)	437, 762, 917, 1378, 2223, 3069
HphI	GGTGA	8 cut(s)	287, 494, 847, 2132, 2342, 2449, 2692, 2759
Hpy166II	GTNNAC	9 cut(s)	927, 1345, 1347, 1456, 1662, 2505, 2837, 2938, 3021
Hpy8I	GTNNAC	9 cut(s)	927, 1345, 1347, 1456, 1662, 2505, 2837, 2938, 3021
HpyCH4III	ACNGT	7 cut(s)	148, 1465, 1691, 2145, 2454, 2810, 3018
HpyCH4IV	ACGT	5 cut(s)	122, 1479, 1721, 2269, 2501
HpyF10VI	GCNNNNNNNGC	5 cut(s)	74, 536, 758, 2061, 2643
HpySE526I	ACGT	5 cut(s)	122, 1479, 1721, 2269, 2501
Hsp92I	GRCGYC	1 cut(s)	2591
HspAI	GCGC	1 cut(s)	286
Kpn2I	TCCGGA	2 cut(s)	2222, 3068
LmnI	GCTCC	5 cut(s)	1355, 1759, 1863, 1950, 3071
Lsp1109I	GCAGC	9 cut(s)	121, 268, 547, 602, 1027, 2306, 2371, 2937, 2984
LweI	GCATC	5 cut(s)	1540, 2250, 2310, 2719, 2812
MabI	ACCWGGT	1 cut(s)	267
MaeI	CTAG	3 cut(s)	1227, 1425, 3063
MaeII	ACGT	5 cut(s)	122, 1479, 1721, 2269, 2501
MboII	GAAGA	9 cut(s)	292, 320, 976, 1091, 1330, 1333, 2030, 2474, 2864
MfeI	CAATTG	1 cut(s)	1107
MflI	RGATCY	3 cut(s)	494, 1957, 2274
MhlI	GDGCHC	5 cut(s)	1226, 1794, 1860, 1947, 2910
MlyI	GAGTC	2 cut(s)	1027, 2685
MmeI	TCCRAC	1 cut(s)	2439
Mph1103I	ATGCAT	1 cut(s)	2407
MroI	TCCGGA	2 cut(s)	2222, 3068
MslI	CAYNNNNRTG	1 cut(s)	1834
MspI	CCGG	6 cut(s)	437, 762, 917, 1378, 2223, 3069
MunI	CAATTG	1 cut(s)	1107
Mva1269I	GAATGC	2 cut(s)	343, 763
MvaI	CCWGG	8 cut(s)	220, 269, 1443, 1484, 1594, 1612, 1730, 2336
MwoI	GCNNNNNNNGC	5 cut(s)	74, 536, 758, 2061, 2643
NciI	CCSGG	2 cut(s)	763, 917
NheI	GCTAGC	1 cut(s)	3062
NlaIV	GGNNCC	3 cut(s)	915, 1965, 2276
NmuCI	GTSAC	8 cut(s)	275, 1475, 2120, 2185, 2330, 2482, 2497, 2680
NsiI	ATGCAT	1 cut(s)	2407
NspI	RCATGY	6 cut(s)	239, 1003, 1143, 2483, 2925, 2995
PaeI	GCATGC	1 cut(s)	2995
PagI	TCATGA	1 cut(s)	3043
PaqCI	CACCTGC	2 cut(s)	1040, 2318
PciI	ACATGT	3 cut(s)	235, 999, 1139
PctI	GAATGC	2 cut(s)	343, 763
PfeI	GAWTC	8 cut(s)	79, 355, 455, 517, 1133, 2154, 2509, 2549
PfoI	TCCNGGA	3 cut(s)	915, 1482, 1728
PinAI	ACCGGT	1 cut(s)	436
PleI	GAGTC	2 cut(s)	1026, 2685
PpsI	GAGTC	2 cut(s)	1026, 2685
Ppu21I	YACGTR	3 cut(s)	1722, 2270, 2502
PscI	ACATGT	3 cut(s)	235, 999, 1139
PshBI	ATTAAT	1 cut(s)	2100
PsiI	TTATAA	2 cut(s)	1574, 2088
Psp124BI	GAGCTC	1 cut(s)	1947
Psp6I	CCWGG	8 cut(s)	218, 267, 1441, 1482, 1592, 1610, 1728, 2334
PspEI	GGTNACC	2 cut(s)	275, 2330
PspFI	CCCAGC	1 cut(s)	857
PspGI	CCWGG	8 cut(s)	218, 267, 1441, 1482, 1592, 1610, 1728, 2334
PspN4I	GGNNCC	3 cut(s)	915, 1965, 2276
PspPI	GGNCC	5 cut(s)	241, 309, 913, 2344, 3049
PstI	CTGCAG	1 cut(s)	258
PstNI	CAGNNNCTG	2 cut(s)	186, 1448
PsuI	RGATCY	3 cut(s)	494, 1957, 2274
RsaI	GTAC	6 cut(s)	823, 1346, 1515, 1688, 2504, 2836
RsaNI	GTAC	6 cut(s)	822, 1345, 1514, 1687, 2503, 2835
RseI	CAYNNNNRTG	1 cut(s)	1834
SacI	GAGCTC	1 cut(s)	1947
Sau96I	GGNCC	5 cut(s)	241, 309, 913, 2344, 3049
ScaI	AGTACT	1 cut(s)	1515
SchI	GAGTC	2 cut(s)	1027, 2685
SduI	GDGCHC	5 cut(s)	1226, 1794, 1860, 1947, 2910
SexAI	ACCWGGT	1 cut(s)	267
SfaNI	GCATC	5 cut(s)	1540, 2250, 2310, 2719, 2812
SfcI	CTRYAG	2 cut(s)	254, 2280
SinI	GGWCC	4 cut(s)	241, 309, 913, 3049
SmiMI	CAYNNNNRTG	1 cut(s)	1834
SmlI	CTYRAG	1 cut(s)	2693
SmoI	CTYRAG	1 cut(s)	2693
SnaBI	TACGTA	1 cut(s)	1722
SphI	GCATGC	1 cut(s)	2995
SsiI	CCGC	5 cut(s)	539, 542, 547, 881, 1746
SspI	AATATT	1 cut(s)	92
SspMI	CTAG	3 cut(s)	1227, 1425, 3063
SstI	GAGCTC	1 cut(s)	1947
StyI	CCWWGG	2 cut(s)	1948, 2845
TaaI	ACNGT	7 cut(s)	148, 1465, 1691, 2145, 2454, 2810, 3018
TaiI	ACGT	5 cut(s)	125, 1482, 1724, 2272, 2504
TaqI	TCGA	6 cut(s)	49, 353, 867, 1273, 2034, 2804
TaqII	GACCGA	1 cut(s)	326
TatI	WGTACW	2 cut(s)	1344, 1513
TauI	GCSGC	1 cut(s)	542
TfiI	GAWTC	8 cut(s)	79, 355, 455, 517, 1133, 2154, 2509, 2549
TscAI	CASTG	5 cut(s)	153, 1799, 2131, 2815, 2832
TseFI	GTSAC	8 cut(s)	275, 1475, 2120, 2185, 2330, 2482, 2497, 2680
TseI	GCWGC	9 cut(s)	109, 256, 560, 615, 1040, 2319, 2384, 2925, 2972
Tsp45I	GTSAC	8 cut(s)	275, 1475, 2120, 2185, 2330, 2482, 2497, 2680
TspGWI	ACGGA	5 cut(s)	1544, 1829, 2223, 2640, 2955
TspRI	CASTG	5 cut(s)	153, 1799, 2131, 2815, 2832
VpaK11BI	GGWCC	4 cut(s)	241, 309, 913, 3049
VspI	ATTAAT	1 cut(s)	2100
XagI	CCTNNNNNAGG	2 cut(s)	1952, 2339
XapI	RAATTY	4 cut(s)	337, 1493, 1914, 2855
XceI	RCATGY	6 cut(s)	239, 1003, 1143, 2483, 2925, 2995
XmiI	GTMKAC	3 cut(s)	926, 1455, 1661
XspI	CTAG	3 cut(s)	1227, 1425, 3063
ZrmI	AGTACT	1 cut(s)	1515
Zsp2I	ATGCAT	1 cut(s)	2407

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.