Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001276

Physical Location & Seq

Genomic Coordinates Reverse (-)

24095162 .. 24099193

Gene Structure 4032 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1148300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3081 bp

ATGGGGTTATTTTGTCATGGACATCTCTTTGCATTTCATCTGAGCAATCGAATTGGTATTGCAAAAGCAAGACTTTTGATTCACATCGAATACTGCACTACCACCTCGCAGCAATCAGAGACGTTCAAACAGGACGATGACGAAACAGTGAGAGAAATCTCCACCGTCCTCAGAAACCACAGCGACTGGCACTTCGTTCTCAACTCAACGGACCTCCCCAGGAAGCTGAACCCACATGTGGTCCGAGCCGTTCTACAGCAGAACCACCAGGTTGGTGACCCAAAACGCCTCCTTAGTTTCTTCCTATGGACCGATACCCACCTGGGTTTTCCCCAAAATTTGCATTCCTTTTCGATTCTTGCTGTTGTTCTGTGCAATTCCAAGATGTTTGAGCAGGCTAATGCTGTGTTAGATAGAATGGTTAAGACCCGAAAACCAGTTTTCGAAGTTTTGGATTGTGTTGTTAGTTGTTTTAGAGATGGTGAGTGTGGTGGGTCTGATGAGATTGTTTTTGAGTATCTGATTAGAGCTTTTAAGGCGGCGGAGCGGTTGAATGAGGCTGCTGATGTTTTTCTGGGACTTAGGAAAGTTGGGATTTTACCAAGATTGGACTGCTGCAATTCTCTGTTGAATGATTTGTTGAAGTATAATAGAATGGAGTTGTTTTGGAAGGTGTATGATGGGATGTTAGAGGCGAAAATGAAACCTGATTTTTACACTTATTACAATGTGATCCATGCGCATTGTAGGGCAGGGAATGCCGGGCAGGGAAAGAGGTTTCTGTTAGAGATGGAGGAGAAGGGTGGTAATCCAGATTTAAGTACCTATAATGTGGTGATTGGTGGTTTGTGTAGGGCTGGGGATGTCGATGAGGCCTTAGCGGTAAAGAAGTCTATGGTGGAGAAGGGATTGGTCCCGGATAGGTATACTTATTCGGCACTTGTTGACGGGTTGTGCAGACAGAAGAGACCAGAAGAAACAAAATTGATTTTGAAATACATGTATGATAAAGGTTTGAGTCCTGACAGCACCTGCTACACTGCTTTGATTGATGGGTTGATGAAAGAAGGTTACTTGGAAGAGGCTCTGAGGATCAAAGATGAGACAATTGCTCGAGGCTTTAAGTTGTGCGATGCCACATGTAATGCAATTTTTGCTGGGATGTGTAAAGTTGGTAGGATGGAGAAGGCAGAAGTTCTCCTGAATGAGATGAATGTTATGGGCACTAGACCTAATGCCCAGACATACAAGTTTTTAATTGACGGGTATTGTCGAGAGAAAAATATGGTCAAGGCTTGTGAGTTACTTAATGAGATGAAGAAGAGGAACTTTGCACCCGGTGTGTTCACTTATGGAGCGATAATCAATGGGCTAAGCCGGCGTGGAGATATGGAAGGGGCTAATCAACTTCTGAAGGAAATGATTACCAGAGGTTTGAAACCAGGTGCCGTTATCTACACAACTGTAATTAGAGGTTACGTCCAGGAAGGAAAATTTGAAGAGGCAATAAAAGTATTAAAAGGAATGACCAAAAAGGGAGTCATGCCTGATGCATTTTGCTACAATTCTCTTATAATTGGCCTCTGTAAAGCCAGGAAAATGGATGAAGCCAGGATTTACTTCGTGGAAATGGTTGACAGAGGATTAAAGCCTAATGCGTATACTTATGGGGCTTTTATTCATGGATACTGTAAGGAGGGGCAAATGCAACTTGCAAATACGTATTTCCAGGAGATGTTGGGTTGCGGTATAGCTCCTAATGATGTTATCTATACAGCCCTGATTGAAGGGCACTGCAAAGACAGTAACTTAACAGAAGCATATTCTACGTTTAGATGTATGCTTGGACGAGGGGTGCTCCCAGATATCAAAACTTATAGTGTCATTATTCATGGTCTCTCAAAGAATGGAAAAATTCAAGAAGCAATGGGGATTTTCTCCGAGCTCCTTGGCAAGGATCTGGTTCCAGATGTTTTTACTTACAGCTCCCTCATATCGGGCTTTTGTAAGCAGGGAAATGTGGATAAGGCTTTCCAACTTCTTGAACAGATGTGCCGGAGAGGCGTTGATCCAAACATTGTTACTTATAATGCCTTGATTAATGGATTGTGTAAGTCAGGTGATACTGATAGAGCCAGAGAACTGTTTGATGGAATCTCCAGAAAGGGTTTGTCTCCTAATGCTGTGACTTATGCTACAATGATGGACGGATATAGCAAATCCGGAAAATTAACCGAGGCATTTCAGTTATTAGATGAGATGCTTCTACGTGGGATCCCTACAGATAGTTTCATCTACTGCATCCTAATTGATGGGTGCTGCAAGGCAGGTGACATGGAGAGGGCCGTGTCCTTATTTCGGGATATAGTGGGGAAGGGCATTGCGGCCACTTCTCCTTTCAATGCATTGATTGATGGCTTCTGCAAATTGGGGAGGATGGTGGAAGCTAACCAGTTGTTGGAAGATATGGTGGATAAGCATGTGACCCCAAATCATGTCACCTACACGATTCTGATTGTTTCCCTCTGCAAAGAGGGGTTGATGAGGGAATCAGAACAGCTCTTTCTGGAAATGCAGAAAAGGAATCTGACGCCAAATATTTTAACATATACTTCACTTTTACACGGATACAATAGCACGGGAAGCAGATATAAGATGTTTGCTCTTTTTGATGAGATGGTGGCGAGGGGACTCAAGCCTGATGAAGTTACATATCGTATGATGGTTGATGCTTATTGCAAAGAAGGTGATTTGGTAAAATGTTTAAAGCTGGTGGATGAAACTTTGGTCAATGGTGCAATCTCCAACAGTGCAGTGGTTGATGCACTGACAAGTACTCTATTCCGAAGGGAAGAATTTTCTGAGATAATGAAGTCACTTGATGAAATGGTAGAACATGGATTTGTGCTCAGTCTTGCGACATGCAGCACATTAGTTCGTGGATTTTACAAATTAGGGAATGCGGAAAAAGCAGCAAGGATTTTTGAGAGCATGCTTAGGTTTGGATGGGTTTCACATTCCACCAGTTTAGATGACTTAATTCATGAGGACCAAAGTGAGGTTAGCTCCGGAATATGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00260 ko00261 ko00270 ko00300 ko01100 ko01110 ko01120 ko01130 ko01210 ko01230 map00260 map00261 map00270 map00300 map01100 map01110 map01120 map01130 map01210 map01230

Pfam Domains

Protein Families

Mem_trans PPR PPR_1 PPR_2 PPR_3 PPR_long

Protein Analysis

1027

Amino Acids

115.18

Weight (kDa)

6.62

Isoelectric Point (pI)

24.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	205 - 250	6.6e-07	PPR repeat family
PPR_long	PF17177	207 - 315	2e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	227 - 281	9.1e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	243 - 285	7.4e-08	PPR repeat family
PPR_1	PF12854	267 - 300	7.9e-10	PPR repeat
PPR_2	PF13041	271 - 320	3.1e-16	PPR repeat family
PPR	PF01535	274 - 304	3e-07	PPR repeat
PPR_1	PF12854	302 - 334	5.4e-06	PPR repeat
PPR_2	PF13041	314 - 355	4.4e-07	PPR repeat family
PPR_2	PF13041	379 - 425	9.7e-14	PPR repeat family
PPR_3	PF13812	400 - 456	2.5e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	408 - 440	1.1e-08	PPR repeat
PPR	PF01535	415 - 444	1.1e-06	PPR repeat
PPR_2	PF13041	419 - 459	1.2e-12	PPR repeat family
PPR_2	PF13041	446 - 482	2.9e-07	PPR repeat family
PPR	PF01535	449 - 479	6.9e-06	PPR repeat
PPR_3	PF13812	470 - 529	3.2e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	477 - 509	5.5e-06	PPR repeat
PPR_2	PF13041	481 - 530	1.9e-15	PPR repeat family
PPR	PF01535	484 - 514	1.3e-07	PPR repeat
PPR_1	PF12854	512 - 544	9.6e-08	PPR repeat
PPR_2	PF13041	516 - 565	3.3e-15	PPR repeat family
PPR	PF01535	520 - 549	1.1e-06	PPR repeat
PPR_3	PF13812	543 - 596	4.4e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	547 - 579	2.2e-07	PPR repeat
PPR	PF01535	554 - 584	8.7e-06	PPR repeat
PPR_long	PF17177	571 - 683	8.1e-09	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	583 - 614	2.4e-06	PPR repeat
PPR_2	PF13041	586 - 635	1.5e-12	PPR repeat family
PPR_1	PF12854	617 - 648	6.6e-06	PPR repeat
PPR_2	PF13041	629 - 670	5.9e-11	PPR repeat family
PPR_3	PF13812	645 - 701	4.3e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	652 - 684	1.2e-15	PPR repeat
PPR_2	PF13041	656 - 705	3.4e-21	PPR repeat family
PPR	PF01535	659 - 689	2.7e-09	PPR repeat
PPR_long	PF17177	672 - 781	1.3e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	680 - 738	5.8e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	687 - 717	2e-12	PPR repeat
PPR_2	PF13041	691 - 740	1.6e-16	PPR repeat family
PPR	PF01535	694 - 724	1.3e-07	PPR repeat
PPR_1	PF12854	722 - 754	1.2e-10	PPR repeat
PPR_2	PF13041	726 - 775	2e-15	PPR repeat family
PPR	PF01535	729 - 759	1.1e-07	PPR repeat
PPR_1	PF12854	757 - 788	1.2e-07	PPR repeat
PPR	PF01535	765 - 794	4.5e-07	PPR repeat
PPR_long	PF17177	769 - 924	1.3e-10	Pentacotripeptide-repeat region of PRORP
PPR	PF01535	800 - 826	5.4e-06	PPR repeat
PPR_1	PF12854	800 - 824	2e-06	PPR repeat
PPR_2	PF13041	800 - 844	1.5e-14	PPR repeat family
PPR_1	PF12854	827 - 858	1.4e-08	PPR repeat
PPR	PF01535	833 - 862	5.4e-07	PPR repeat
PPR_2	PF13041	838 - 877	2.1e-09	PPR repeat family
PPR_3	PF13812	854 - 913	5.6e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	885 - 914	9.6e-08	PPR repeat family
PPR_1	PF12854	896 - 927	7e-08	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001276)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61990
fragaria_vesca	FvH4_5g09340 FvH4_5g09340 FvH4_5g09340 FvH4_5g09340
malus_domestica	MD06G1131900.v1.1 MD14G1148300.v1.1
prunus_persica	Prupe.5G142900_v2.0.a1 Prupe.5G142900_v2.0.a1
pyrus_communis	pycom06g12350 pycom14g12300
rosa_chinensis	RchiOBHm_Chr1g0325241 RchiOBHm_Chr1g0325261 RchiOBHm_Chr2g0162681 RchiOBHm_Chr7g0188811 RchiOBHm_Chr7g0229441
rosa_laevigata	RLG00000004657 RLG00000028121 RLG00000028122 RLG00000031426 RLG00000035825
rosa_multiflora	Rmu_sc0001621.1_g000013 Rmu_sc0001918.1_g000006 Rmu_sc0004279.1_g000018 Rmu_sc0034360.1_g000001 Rmu_sc0034361.1_g000001
rosa_roxburghii	Rroxscaffold_3G00266000 Rroxscaffold_5G00381140
rosa_rugosa	Rorug02G0360500 Rorug05G0369000 Rorug06G0494100 Rorug07G0156300
rosa_samantha	Rh1BG033100 Rh2CG348200 Rh2CG348300 Rh4AG001800 Rh4AG163800 Rh4BG001600 Rh4BG400000 Rh4BG400100 Rh4CG415000 Rh4CG415100 Rh6DG332400 Rh6DG332500 Rh7AG098900 Rh7AG408500 Rh7BG100600 Rh7CG102100 Rh7CG477400 Rh7DG100700 Rh7DG406300 Rh7DG406400 Rh7DG446700
rosa_wichuraiana	Rw0G022150 Rw7G008520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1574, 2088
AarI	CACCTGC	2 cut(s)	1040, 2318
Acc16I	TGCGCA	1 cut(s)	741
Acc36I	ACCTGC	2 cut(s)	1040, 2318
AccB1I	GGYRCC	1 cut(s)	1445
AccB7I	CCANNNNNTGG	1 cut(s)	2458
AccBSI	CCGCTC	1 cut(s)	547
AccI	GTMKAC	2 cut(s)	926, 1661
AccIII	TCCGGA	2 cut(s)	2222, 3068
AciI	CCGC	7 cut(s)	539, 542, 547, 881, 1746, 2384, 2963
AclWI	GGATC	6 cut(s)	727, 1100, 1965, 2063, 2269, 2282
AcoI	YGGCCR	1 cut(s)	2385
AcsI	RAATTY	4 cut(s)	337, 1493, 1914, 2855
AcuI	CTGAAG	1 cut(s)	1433
AcyI	GRCGYC	1 cut(s)	2591
AdeI	CACNNNGTG	1 cut(s)	1340
AfaI	GTAC	2 cut(s)	823, 2836
AfiI	CCNNNNNNNGG	6 cut(s)	238, 1954, 1996, 2358, 2458, 3058
AflIII	ACRYGT	3 cut(s)	235, 999, 1139
AjnI	CCWGG	8 cut(s)	218, 267, 321, 1441, 1482, 1592, 1610, 1728
AluBI	AGCT	9 cut(s)	226, 530, 1754, 1945, 1986, 2447, 2560, 2770, 3066
AluI	AGCT	9 cut(s)	226, 530, 1754, 1945, 1986, 2447, 2560, 2770, 3066
Alw21I	GWGCWC	3 cut(s)	1860, 1947, 2910
Alw26I	GTCTC	5 cut(s)	113, 961, 1097, 1901, 2178
AlwI	GGATC	6 cut(s)	727, 1100, 1965, 2063, 2269, 2282
AlwNI	CAGNNNCTG	2 cut(s)	186, 1032
Ama87I	CYCGRG	1 cut(s)	1113
Aor13HI	TCCGGA	2 cut(s)	2222, 3068
AoxI	GGCC	4 cut(s)	873, 1579, 2343, 2385
ApeKI	GCWGC	6 cut(s)	109, 560, 615, 2319, 2925, 2972
ApoI	RAATTY	4 cut(s)	337, 1493, 1914, 2855
ArsI	GACNNNNNNTTYG	4 cut(s)	176, 208, 1524, 1556
AseI	ATTAAT	1 cut(s)	2100
AspLEI	GCGC	1 cut(s)	742
AspS9I	GGNCC	6 cut(s)	211, 241, 309, 913, 2343, 3049
AsuC2I	CCSGG	3 cut(s)	763, 917, 1338
AsuHPI	GGTGA	7 cut(s)	287, 494, 847, 2132, 2342, 2491, 2759
AsuII	TTCGAA	1 cut(s)	444
AvaI	CYCGRG	1 cut(s)	1113
AvaII	GGWCC	5 cut(s)	211, 241, 309, 913, 3049
BaeGI	GKGCMC	2 cut(s)	1226, 1794
BamHI	GGATCC	1 cut(s)	2274
BanI	GGYRCC	1 cut(s)	1445
BanII	GRGCYC	1 cut(s)	1947
Bbv12I	GWGCWC	3 cut(s)	1860, 1947, 2910
BbvI	GCAGC	6 cut(s)	121, 547, 602, 2306, 2937, 2984
BceAI	ACGGC	3 cut(s)	233, 1433, 2330
BciT130I	CCWGG	8 cut(s)	220, 269, 323, 1443, 1484, 1594, 1612, 1730
BciVI	GTATCC	2 cut(s)	1679, 2621
BcnI	CCSGG	3 cut(s)	763, 917, 1338
BcoDI	GTCTC	5 cut(s)	113, 961, 1097, 1901, 2178
BfaI	CTAG	1 cut(s)	1227
BfmI	CTRYAG	2 cut(s)	254, 2280
BfuAI	ACCTGC	2 cut(s)	1040, 2318
BfuI	GTATCC	2 cut(s)	1679, 2621
BglI	GCCNNNNNGGC	1 cut(s)	2061
BisI	GCNGC	8 cut(s)	110, 540, 561, 616, 2320, 2385, 2926, 2973
BlpI	GCTNAGC	1 cut(s)	1373
BlsI	GCNGC	8 cut(s)	111, 541, 562, 617, 2321, 2386, 2927, 2974
BmcAI	AGTACT	1 cut(s)	2836
Bme18I	GGWCC	5 cut(s)	211, 241, 309, 913, 3049
BmeT110I	CYCGRG	1 cut(s)	1113
BmgT120I	GGNCC	6 cut(s)	211, 241, 309, 913, 2343, 3049
BmiI	GGNNCC	4 cut(s)	915, 1447, 1965, 2276
BmsI	GCATC	6 cut(s)	1123, 1540, 2250, 2310, 2719, 2812
BplI	GAGNNNNNCTC	3 cut(s)	1842, 1874, 3050
BpmI	CTGGAG	1 cut(s)	2143
Bpu10I	CCTNAGC	2 cut(s)	877, 2996
Bpu1102I	GCTNAGC	1 cut(s)	1373
Bpu14I	TTCGAA	1 cut(s)	444
BpuEI	CTTGAG	1 cut(s)	2678
BpuMI	CCSGG	3 cut(s)	763, 917, 1338
BsaAI	YACGTR	2 cut(s)	1722, 2270
BsaBI	GATNNNNATC	1 cut(s)	83
BsaHI	GRCGYC	1 cut(s)	2591
BsaI	GGTCTC	2 cut(s)	961, 1901
BsaJI	CCNNGG	4 cut(s)	218, 322, 1948, 2235
BsaWI	WCCGGW	2 cut(s)	2222, 3068
Bsc4I	CCNNNNNNNGG	6 cut(s)	238, 1954, 1996, 2358, 2458, 3058
Bse118I	RCCGGY	1 cut(s)	1377
Bse1I	ACTGG	4 cut(s)	191, 437, 2452, 3024
Bse3DI	GCAATG	2 cut(s)	1932, 2379
Bse8I	GATNNNNATC	1 cut(s)	83
BseAI	TCCGGA	2 cut(s)	2222, 3068
BseBI	CCWGG	8 cut(s)	220, 269, 323, 1443, 1484, 1594, 1612, 1730
BseDI	CCNNGG	4 cut(s)	218, 322, 1948, 2235
BseGI	GGATG	9 cut(s)	690, 868, 1167, 1185, 1609, 2301, 2442, 2782, 3011
BseJI	GATNNNNATC	1 cut(s)	83
BseLI	CCNNNNNNNGG	6 cut(s)	238, 1954, 1996, 2358, 2458, 3058
BseMI	GCAATG	2 cut(s)	1932, 2379
BseMII	CTCAG	5 cut(s)	32, 184, 1079, 2853, 2923
BseNI	ACTGG	4 cut(s)	191, 437, 2452, 3024
BseRI	GAGGAG	1 cut(s)	809
BseSI	GKGCMC	2 cut(s)	1226, 1794
BseXI	GCAGC	6 cut(s)	121, 547, 602, 2306, 2937, 2984
BseYI	CCCAGC	2 cut(s)	857, 1157
BsgI	GTGCAG	3 cut(s)	79, 976, 2832
BshFI	GGCC	4 cut(s)	875, 1581, 2345, 2387
BshNI	GGYRCC	1 cut(s)	1445
BsiHKAI	GWGCWC	3 cut(s)	1860, 1947, 2910
BsiHKCI	CYCGRG	1 cut(s)	1113
BsiSI	CCGG	7 cut(s)	762, 917, 1338, 1378, 2056, 2223, 3069
BslFI	GGGAC	3 cut(s)	591, 899, 2703
BslI	CCNNNNNNNGG	6 cut(s)	238, 1954, 1996, 2358, 2458, 3058
BsmAI	GTCTC	5 cut(s)	113, 961, 1097, 1901, 2178
BsmBI	CGTCTC	1 cut(s)	113
BsmFI	GGGAC	3 cut(s)	591, 899, 2703
BsmI	GAATGC	3 cut(s)	343, 763, 2965
BsnI	GGCC	4 cut(s)	875, 1581, 2345, 2387
Bso31I	GGTCTC	2 cut(s)	961, 1901
BsoBI	CYCGRG	1 cut(s)	1113
Bsp119I	TTCGAA	1 cut(s)	444
Bsp1286I	GDGCHC	5 cut(s)	1226, 1794, 1860, 1947, 2910
Bsp13I	TCCGGA	2 cut(s)	2222, 3068
Bsp143I	GATC	5 cut(s)	732, 1092, 1957, 2068, 2274
Bsp1720I	GCTNAGC	1 cut(s)	1373
BspACI	CCGC	7 cut(s)	539, 542, 547, 881, 1746, 2384, 2963
BspANI	GGCC	4 cut(s)	875, 1581, 2345, 2387
BspCNI	CTCAG	5 cut(s)	33, 183, 1080, 2854, 2922
BspEI	TCCGGA	2 cut(s)	2222, 3068
BspHI	TCATGA	1 cut(s)	3043
BspLI	GGNNCC	4 cut(s)	915, 1447, 1965, 2276
BspMI	ACCTGC	2 cut(s)	1040, 2318
BspPI	GGATC	6 cut(s)	727, 1100, 1965, 2063, 2269, 2282
BspT104I	TTCGAA	1 cut(s)	444
BspT107I	GGYRCC	1 cut(s)	1445
BspTNI	GGTCTC	2 cut(s)	961, 1901
BsrBI	CCGCTC	1 cut(s)	547
BsrDI	GCAATG	2 cut(s)	1932, 2379
BsrFI	RCCGGY	1 cut(s)	1377
BsrI	ACTGG	4 cut(s)	191, 437, 2452, 3024
BssAI	RCCGGY	1 cut(s)	1377
BssECI	CCNNGG	4 cut(s)	218, 322, 1948, 2235
BssMI	GATC	5 cut(s)	732, 1092, 1957, 2068, 2274
BssNAI	GTATAC	2 cut(s)	927, 1662
BssNI	GRCGYC	1 cut(s)	2591
BssT1I	CCWWGG	1 cut(s)	1948
Bst1107I	GTATAC	2 cut(s)	927, 1662
Bst2UI	CCWGG	8 cut(s)	220, 269, 323, 1443, 1484, 1594, 1612, 1730
Bst4CI	ACNGT	7 cut(s)	148, 166, 1465, 1691, 1805, 2145, 2810
Bst6I	CTCTTC	4 cut(s)	959, 1074, 1316, 1494
BstACI	GRCGYC	1 cut(s)	2591
BstAPI	GCANNNNNTGC	2 cut(s)	758, 1154
BstBAI	YACGTR	2 cut(s)	1722, 2270
BstBI	TTCGAA	1 cut(s)	444
BstC8I	GCNNGC	3 cut(s)	396, 1379, 2993
BstEII	GGTNACC	1 cut(s)	275
BstENI	CCTNNNNNAGG	1 cut(s)	1952
BstF5I	GGATG	9 cut(s)	690, 868, 1167, 1185, 1609, 2301, 2442, 2782, 3011
BstHHI	GCGC	1 cut(s)	742
BstKTI	GATC	5 cut(s)	735, 1095, 1960, 2071, 2277
BstMAI	GTCTC	5 cut(s)	113, 961, 1097, 1901, 2178
BstMBI	GATC	5 cut(s)	732, 1092, 1957, 2068, 2274
BstMWI	GCNNNNNNNGC	7 cut(s)	536, 758, 1154, 1378, 2061, 2643, 2969
BstNI	CCWGG	8 cut(s)	220, 269, 323, 1443, 1484, 1594, 1612, 1730
BstNSI	RCATGY	6 cut(s)	239, 1003, 1143, 2483, 2925, 2995
BstPI	GGTNACC	1 cut(s)	275
BstSFI	CTRYAG	2 cut(s)	254, 2280
BstSLI	GKGCMC	2 cut(s)	1226, 1794
BstSNI	TACGTA	1 cut(s)	1722
BstV1I	GCAGC	6 cut(s)	121, 547, 602, 2306, 2937, 2984
BstX2I	RGATCY	2 cut(s)	1957, 2274
BstXI	CCANNNNNNTGG	2 cut(s)	272, 1600
BstYI	RGATCY	2 cut(s)	1957, 2274
BstZ17I	GTATAC	2 cut(s)	927, 1662
BsuI	GTATCC	2 cut(s)	1679, 2621
BsuRI	GGCC	4 cut(s)	875, 1581, 2345, 2387
BtgZI	GCGATG	1 cut(s)	1146
BtsCI	GGATG	9 cut(s)	690, 868, 1167, 1185, 1609, 2301, 2442, 2782, 3011
BtsI	GCAGTG	3 cut(s)	1038, 1792, 2820
BtsIMutI	CAGTG	6 cut(s)	153, 1038, 1792, 2815, 2820, 2825
BveI	ACCTGC	2 cut(s)	1040, 2318
Cac8I	GCNNGC	3 cut(s)	396, 1379, 2993
CaiI	CAGNNNCTG	2 cut(s)	186, 1032
CciI	TCATGA	1 cut(s)	3043
CfoI	GCGC	1 cut(s)	742
Cfr10I	RCCGGY	1 cut(s)	1377
Cfr13I	GGNCC	6 cut(s)	211, 241, 309, 913, 2343, 3049
CseI	GACGC	1 cut(s)	2599
CsiI	ACCWGGT	2 cut(s)	267, 1441
Csp6I	GTAC	2 cut(s)	822, 2835
CviQI	GTAC	2 cut(s)	822, 2835
DpnI	GATC	5 cut(s)	734, 1094, 1959, 2070, 2276
DpnII	GATC	5 cut(s)	732, 1092, 1957, 2068, 2274
DraI	TTTAAA	1 cut(s)	2766
DraIII	CACNNNGTG	1 cut(s)	1340
EaeI	YGGCCR	1 cut(s)	2385
Eam1104I	CTCTTC	4 cut(s)	959, 1074, 1316, 1494
EarI	CTCTTC	4 cut(s)	959, 1074, 1316, 1494
EciI	GGCGGA	1 cut(s)	557
Ecl136II	GAGCTC	1 cut(s)	1945
Eco105I	TACGTA	1 cut(s)	1722
Eco130I	CCWWGG	1 cut(s)	1948
Eco147I	AGGCCT	1 cut(s)	875
Eco24I	GRGCYC	1 cut(s)	1947
Eco31I	GGTCTC	2 cut(s)	961, 1901
Eco32I	GATATC	1 cut(s)	1867
Eco47I	GGWCC	5 cut(s)	211, 241, 309, 913, 3049
Eco53kI	GAGCTC	1 cut(s)	1945
Eco57I	CTGAAG	1 cut(s)	1433
Eco88I	CYCGRG	1 cut(s)	1113
Eco91I	GGTNACC	1 cut(s)	275
EcoICRI	GAGCTC	1 cut(s)	1945
EcoNI	CCTNNNNNAGG	1 cut(s)	1952
EcoO65I	GGTNACC	1 cut(s)	275
EcoRII	CCWGG	8 cut(s)	218, 267, 321, 1441, 1482, 1592, 1610, 1728
EcoRV	GATATC	1 cut(s)	1867
EcoT14I	CCWWGG	1 cut(s)	1948
EcoT22I	ATGCAT	2 cut(s)	1555, 2407
EcoT38I	GRGCYC	1 cut(s)	1947
ErhI	CCWWGG	1 cut(s)	1948
Esp3I	CGTCTC	1 cut(s)	113
FalI	AAGNNNNNCTT	4 cut(s)	57, 89, 1313, 1345
FaqI	GGGAC	3 cut(s)	591, 899, 2703
FblI	GTMKAC	2 cut(s)	926, 1661
Fnu4HI	GCNGC	8 cut(s)	110, 540, 561, 616, 2320, 2385, 2926, 2973
FokI	GGATG	9 cut(s)	697, 875, 1174, 1192, 1616, 2288, 2449, 2789, 3018
FriOI	GRGCYC	1 cut(s)	1947
Fsp4HI	GCNGC	8 cut(s)	110, 540, 561, 616, 2320, 2385, 2926, 2973
FspAI	RTGCGCAY	1 cut(s)	741
FspBI	CTAG	1 cut(s)	1227
FspI	TGCGCA	1 cut(s)	741
GlaI	GCGC	1 cut(s)	741
GluI	GCNGC	8 cut(s)	110, 540, 561, 616, 2320, 2385, 2926, 2973
GsaI	CCCAGC	2 cut(s)	861, 1161
GsuI	CTGGAG	1 cut(s)	2143
HaeIII	GGCC	4 cut(s)	875, 1581, 2345, 2387
HapII	CCGG	7 cut(s)	762, 917, 1338, 1378, 2056, 2223, 3069
HgaI	GACGC	1 cut(s)	2599
HhaI	GCGC	1 cut(s)	742
Hin1I	GRCGYC	1 cut(s)	2591
Hin6I	GCGC	1 cut(s)	740
HinP1I	GCGC	1 cut(s)	740
HincII	GTYRAC	2 cut(s)	946, 1636
HindII	GTYRAC	2 cut(s)	946, 1636
HinfI	GANTC	9 cut(s)	79, 355, 1018, 1539, 2154, 2509, 2549, 2584, 2691
HpaII	CCGG	7 cut(s)	762, 917, 1338, 1378, 2056, 2223, 3069
HphI	GGTGA	7 cut(s)	287, 494, 847, 2132, 2342, 2491, 2759
Hpy166II	GTNNAC	5 cut(s)	927, 946, 1347, 1636, 1662
Hpy8I	GTNNAC	5 cut(s)	927, 946, 1347, 1636, 1662
HpyCH4III	ACNGT	7 cut(s)	148, 166, 1465, 1691, 1805, 2145, 2810
HpyCH4IV	ACGT	5 cut(s)	122, 1479, 1721, 1829, 2269
HpyF10VI	GCNNNNNNNGC	7 cut(s)	536, 758, 1154, 1378, 2061, 2643, 2969
HpySE526I	ACGT	5 cut(s)	122, 1479, 1721, 1829, 2269
Hsp92I	GRCGYC	1 cut(s)	2591
HspAI	GCGC	1 cut(s)	740
Kpn2I	TCCGGA	2 cut(s)	2222, 3068
KroI	GCCGGC	1 cut(s)	1377
KroNI	GCCGGC	1 cut(s)	1379
Kzo9I	GATC	5 cut(s)	732, 1092, 1957, 2068, 2274
LmnI	GCTCC	7 cut(s)	544, 1355, 1759, 1863, 1950, 1991, 3071
Lsp1109I	GCAGC	6 cut(s)	121, 547, 602, 2306, 2937, 2984
LweI	GCATC	6 cut(s)	1123, 1540, 2250, 2310, 2719, 2812
MabI	ACCWGGT	2 cut(s)	267, 1441
MaeI	CTAG	1 cut(s)	1227
MaeII	ACGT	5 cut(s)	122, 1479, 1721, 1829, 2269
MalI	GATC	5 cut(s)	734, 1094, 1959, 2070, 2276
MbiI	CCGCTC	1 cut(s)	547
MboI	GATC	5 cut(s)	732, 1092, 1957, 2068, 2274
MboII	GAAGA	9 cut(s)	292, 976, 986, 1091, 1330, 1333, 1511, 2474, 2864
MfeI	CAATTG	1 cut(s)	1107
MflI	RGATCY	2 cut(s)	1957, 2274
MhlI	GDGCHC	5 cut(s)	1226, 1794, 1860, 1947, 2910
MlyI	GAGTC	3 cut(s)	1027, 1548, 2685
MmeI	TCCRAC	3 cut(s)	2059, 2439, 2829
Mph1103I	ATGCAT	2 cut(s)	1555, 2407
MroI	TCCGGA	2 cut(s)	2222, 3068
MroNI	GCCGGC	1 cut(s)	1377
MspI	CCGG	7 cut(s)	762, 917, 1338, 1378, 2056, 2223, 3069
MunI	CAATTG	1 cut(s)	1107
Mva1269I	GAATGC	3 cut(s)	343, 763, 2965
MvaI	CCWGG	8 cut(s)	220, 269, 323, 1443, 1484, 1594, 1612, 1730
MwoI	GCNNNNNNNGC	7 cut(s)	536, 758, 1154, 1378, 2061, 2643, 2969
NaeI	GCCGGC	1 cut(s)	1379
NciI	CCSGG	3 cut(s)	763, 917, 1338
NdeII	GATC	5 cut(s)	732, 1092, 1957, 2068, 2274
NgoMIV	GCCGGC	1 cut(s)	1377
NlaIV	GGNNCC	4 cut(s)	915, 1447, 1965, 2276
NmuCI	GTSAC	6 cut(s)	275, 2185, 2330, 2482, 2497, 2874
NsbI	TGCGCA	1 cut(s)	741
NsiI	ATGCAT	2 cut(s)	1555, 2407
NspI	RCATGY	6 cut(s)	239, 1003, 1143, 2483, 2925, 2995
NspV	TTCGAA	1 cut(s)	444
PaeI	GCATGC	1 cut(s)	2995
PaeR7I	CTCGAG	1 cut(s)	1113
PagI	TCATGA	1 cut(s)	3043
PaqCI	CACCTGC	2 cut(s)	1040, 2318
PceI	AGGCCT	1 cut(s)	875
PciI	ACATGT	3 cut(s)	235, 999, 1139
PctI	GAATGC	3 cut(s)	343, 763, 2965
PdiI	GCCGGC	1 cut(s)	1379
PfeI	GAWTC	6 cut(s)	79, 355, 2154, 2509, 2549, 2584
PflMI	CCANNNNNTGG	1 cut(s)	2458
PfoI	TCCNGGA	3 cut(s)	915, 1482, 1728
PkrI	GCNGC	8 cut(s)	111, 541, 562, 617, 2321, 2386, 2927, 2974
PleI	GAGTC	3 cut(s)	1026, 1547, 2685
PpsI	GAGTC	3 cut(s)	1026, 1547, 2685
Ppu21I	YACGTR	2 cut(s)	1722, 2270
PscI	ACATGT	3 cut(s)	235, 999, 1139
PshBI	ATTAAT	1 cut(s)	2100
PsiI	TTATAA	2 cut(s)	1574, 2088
Psp124BI	GAGCTC	1 cut(s)	1947
Psp6I	CCWGG	8 cut(s)	218, 267, 321, 1441, 1482, 1592, 1610, 1728
PspEI	GGTNACC	1 cut(s)	275
PspFI	CCCAGC	2 cut(s)	857, 1157
PspGI	CCWGG	8 cut(s)	218, 267, 321, 1441, 1482, 1592, 1610, 1728
PspN4I	GGNNCC	4 cut(s)	915, 1447, 1965, 2276
PspPI	GGNCC	6 cut(s)	211, 241, 309, 913, 2343, 3049
PspXI	VCTCGAGB	1 cut(s)	1113
PstNI	CAGNNNCTG	2 cut(s)	186, 1032
PsuI	RGATCY	2 cut(s)	1957, 2274
RsaI	GTAC	2 cut(s)	823, 2836
RsaNI	GTAC	2 cut(s)	822, 2835
SacI	GAGCTC	1 cut(s)	1947
SatI	GCNGC	8 cut(s)	110, 540, 561, 616, 2320, 2385, 2926, 2973
Sau3AI	GATC	5 cut(s)	732, 1092, 1957, 2068, 2274
Sau96I	GGNCC	6 cut(s)	211, 241, 309, 913, 2343, 3049
ScaI	AGTACT	1 cut(s)	2836
SchI	GAGTC	3 cut(s)	1027, 1548, 2685
SduI	GDGCHC	5 cut(s)	1226, 1794, 1860, 1947, 2910
SexAI	ACCWGGT	2 cut(s)	267, 1441
SfaNI	GCATC	6 cut(s)	1123, 1540, 2250, 2310, 2719, 2812
SfcI	CTRYAG	2 cut(s)	254, 2280
Sfr274I	CTCGAG	1 cut(s)	1113
SfuI	TTCGAA	1 cut(s)	444
SinI	GGWCC	5 cut(s)	211, 241, 309, 913, 3049
SlaI	CTCGAG	1 cut(s)	1113
SmlI	CTYRAG	2 cut(s)	1113, 2693
SmoI	CTYRAG	2 cut(s)	1113, 2693
SnaBI	TACGTA	1 cut(s)	1722
SphI	GCATGC	1 cut(s)	2995
SseBI	AGGCCT	1 cut(s)	875
SsiI	CCGC	7 cut(s)	539, 542, 547, 881, 1746, 2384, 2963
SspI	AATATT	1 cut(s)	2599
SspMI	CTAG	1 cut(s)	1227
SstI	GAGCTC	1 cut(s)	1947
StuI	AGGCCT	1 cut(s)	875
StyI	CCWWGG	1 cut(s)	1948
TaaI	ACNGT	7 cut(s)	148, 166, 1465, 1691, 1805, 2145, 2810
TaiI	ACGT	5 cut(s)	125, 1482, 1724, 1832, 2272
TaqI	TCGA	7 cut(s)	49, 87, 353, 444, 867, 1114, 1273
TaqII	GACCGA	1 cut(s)	326
TatI	WGTACW	1 cut(s)	2834
TauI	GCSGC	2 cut(s)	542, 2387
TfiI	GAWTC	6 cut(s)	79, 355, 2154, 2509, 2549, 2584
TscAI	CASTG	6 cut(s)	153, 1045, 1799, 2815, 2820, 2832
TseFI	GTSAC	6 cut(s)	275, 2185, 2330, 2482, 2497, 2874
TseI	GCWGC	6 cut(s)	109, 560, 615, 2319, 2925, 2972
Tsp45I	GTSAC	6 cut(s)	275, 2185, 2330, 2482, 2497, 2874
TspGWI	ACGGA	3 cut(s)	224, 2223, 2640
TspRI	CASTG	6 cut(s)	153, 1045, 1799, 2815, 2820, 2832
Van91I	CCANNNNNTGG	1 cut(s)	2458
VpaK11BI	GGWCC	5 cut(s)	211, 241, 309, 913, 3049
VspI	ATTAAT	1 cut(s)	2100
XagI	CCTNNNNNAGG	1 cut(s)	1952
XapI	RAATTY	4 cut(s)	337, 1493, 1914, 2855
XceI	RCATGY	6 cut(s)	239, 1003, 1143, 2483, 2925, 2995
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2812
XhoI	CTCGAG	1 cut(s)	1113
XmiI	GTMKAC	2 cut(s)	926, 1661
XspI	CTAG	1 cut(s)	1227
ZrmI	AGTACT	1 cut(s)	2836
Zsp2I	ATGCAT	2 cut(s)	1555, 2407

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.