Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0001276

Physical Location & Seq

Genomic Coordinates Reverse (-)

7758593 .. 7761653

Gene Structure 3061 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G008520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3024 bp

ATGGGTTTAGCTTCACATGGGAGAAACCTTGCATTTCAGATTGGTAATCCAAAATCAAGGCTTTTGATTCGGGTCCACTGCTGCAGTTATCAATCAGACAGTAAAAAGCAAGAGGATGATGAAACAGTGAGGGAAATCTGTACCATTCTGAAGCGAAGCAGAGAGTGGGAGTCCGTGTTGAGCTCATCAGGGTTTCCCAACAAGCTAAACCCTCGTGTGGTTCGATCCGTTCTTCAACAACACCACCAGGTTGGTGACCCAGAAAGACTTTTAAGTTTCTTTGATTGGTCTGGTACCCAATTGGGTGTGCTCCCAAATTTGCATTCTTTTTCGATTATGGCTCTTGTTTTGTGTAATTCCAAGCTTTTTGGGCATGCCCGTGGTGTGTTGGAGAGAATGGTTAGGACCCGGAAGCCGGCTTTAGAAGTTTTGGATTCTGTTGTTAGGTGTTTTAGACAGTTTGAGGGGTCTGATATGGTGGTTTTTGAGATTTTGATCAATGTGTTTAGGATGGGGGGTTGGTTTTATGAGGCTGCTGATGTGTTTTTGGGTGTTACGAGTGTTGGGATTTCGCCCAGATTGGAATGCTGTAATTCTCTGTTGAGTGACTTGTTGAAGGGTAATAGGATGAGGCTGTTCTGGAAAGTGTATGGCAGGATGTTAGAGGCTAAGATTGAGCCGGATTTTTACACTTACAGCAATGTGATCAACGCACATTGTAAGTTTGGGGATGTTAGGCAGGGTAAGAGGCTACTGTTTGAGATGGTACAGAAGGGCTGTAATCCGAATTTGAGTACCTTCAATGTGGTGATTGATGGATTGTGTACAAGTAGGGCTGTTGATGAAGCCATTGAGTTGAAAAAATCAATGGTTGAGAAGGGATTGACCCCTGATAGGTATACTTATTCAATACTAGTTGATGGATTGTGCAGACAGAAGAGATCAGAAGAAGCAAAGATGGTATTGAACGAGATGTATGATATAGGTTTGAATCCTGACCGCACCTTGTACATTGCTTTGATTGATGGATTCTTGAAAGAAAGTAACGTGGATAAGGCCCTGAGGATCAAAGAAGAGATGGTCGCTCGAGGAGTTAAGTTGTGCAGTGTCACATATAATGTGATTTTTGCAGGGATCTGTAAAATAGGTAAGATGAAGAAGGCAGAATCTCTTTTGAATGAGATGAATGCTATGGGAATTGAACCTAATACCCAGACATATAACTATTTAATTGATGGGTACTGTCGGGAGCAAAATGTAAACAAGGGTTATGCATTACTTAATGAGATGAAGCAAAGGAACTTGACACCCAATGAGGTCACTTGTGGAGTGGTAATCAATGGCCTATGCCTGGTAATGATCACTGGGGGTTTGAAACCCGGTAGAGTTATCTACACAACTCTAATAAAAGGTCATCTACAAGAAAGAAAATTTGAAGAGGCGATAAAACTATTGAAGGAAATGAGTGAAAAAGGTGTCATTCCTGATGTCTTTTGCTACAATTCTCTTGTAATTGGCCTGTGCAAAGCGAGGAAATTTGAAGAAGCCAACACTTATTTGGTGGAAATGGTTGAGAGAGGATTAAAACCTAATGCATATACTTATGGGGCTTTTGTTCACGGGTACTGTAAGGAGAGGGAAATGCAACTCGCGAATAGGTATTTCGAGGAGATGCTAGGTTCTGGTATAGCTCCAAATGAGTTTATCTATACAGCCCTGATTGAAGGACACTGCAAAGAGGGTAACTTGGTTGAAGCTCTTTCAACATTTAGATCCATGTTGGGACGCGGGGTGACCCCAGATATCAAAACATACAGTGTCATTATTCATGGTCTCTCCAGGAGAGGAAAACTTGAAGAAGCAATGGGGATCTTCTCTGAGCTCCTTGGCAAGAATTTGGTTCCGGATGTATTTACATACAGCTCTCTCATATCTGGCTTTTGCAAGCAGGGAAATGCAGAGAAGTCTTTCCAACTTCTTGAACAGATGTCTCAGAGAGGTATTAAACCAAACATTGTCACTTACAACGGCTTGATTAATGGATTGTGCAAGTCTGGGGACATTGACAGAGCCAGAGAAGTGTTTGATGCAATTTCAGGAAAGGGTTTACCTCCTAATGCTGTGACTTATGCTACAATGATGGACGGATATAGCAAATCTGGAAAATTAACTGAGGCTTTACAATTATTAGATAAGATGCAACTCCATGGGATTCCTACTGATAGTTTCATTTACTGCACCCTGATTGATGGGTTCTGCATGGCAGGTGATACCGACAAGGCCTTGTCTTTATTTAATGAAGTGGTGGATAAGGGCCTTGCTGCCACCTCTTCTTTCAATGCATTGATTGATGGCTTCTGTAAATTGGGGAAGCAGATGGAAGCCAAACAGTTGTTTGAAGATATGGTGGATAAGCATGTGACACCAAATCATGTTACGTACACGATTCTGATTGATTCTCTCTGCAAAGAGGGCTTGTTGAGGGAATCAGAAGAGCTATTTCTGGAAATGCAGGAAAGGAATCTGCAGGCAAATATTACAACCTACACTTCACTTTTGCAGGGCTACAACAGCACTGGAAGCAGAGTAAAGATGTTTGCTCTTTACGAGGAAATGATAGCAAGGGGACTCAAACCTGATGAAATAACCTATGGTCTGATGGTCGAGGCCTATTGCGAAGAAGGTGATGTGGTAAAGTCTCTAAAGCTAGTGGATGAATGTTTGGTCAATACTGTTAATTTGAACAGTGATGTGGTAGATGCTCTGGCAAATGCCCTATTCCAAAAGGCAGAATTTTCAAAAGTGATGAAGTCACTTGATGAAATGGGAGAGCAGGGATTTGTGCTCAGTCTCGCTACATGTAAAACATTAGTTCACGGATTTTTCTACAAATCAGGGAATGCAGAGAGGGCAGCAAGGATTCTCAAGAGCATGCTCAGGTTTGGATGGGTTTCGGAGTCCACAAGTATAAGCGACTTAATTAATAATGAAGAACAAAATGAGGCAAGCTCTGGGATTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00260 ko00261 ko00270 ko00300 ko01100 ko01110 ko01120 ko01130 ko01210 ko01230 map00260 map00261 map00270 map00300 map01100 map01110 map01120 map01130 map01210 map01230

Pfam Domains

Protein Families

Mem_trans PPR PPR_1 PPR_2 PPR_3 PPR_long

Protein Analysis

1007

Amino Acids

112.85

Weight (kDa)

6.5

Isoelectric Point (pI)

29.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	196 - 241	2.4e-08	PPR repeat family
PPR_long	PF17177	198 - 306	8.1e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	222 - 272	2.2e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	231 - 275	1.5e-11	PPR repeat family
PPR_1	PF12854	258 - 291	4.4e-08	PPR repeat
PPR_2	PF13041	266 - 311	4.3e-15	PPR repeat family
PPR_1	PF12854	293 - 325	7e-10	PPR repeat
PPR_3	PF13812	355 - 414	6.4e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	368 - 416	6.3e-17	PPR repeat family
PPR_3	PF13812	390 - 446	7.5e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	398 - 431	2.6e-10	PPR repeat
PPR_2	PF13041	402 - 450	5e-16	PPR repeat family
PPR	PF01535	406 - 434	1e-05	PPR repeat
PPR_3	PF13812	457 - 508	9.6e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	460 - 509	6.9e-15	PPR repeat family
PPR	PF01535	463 - 493	3.5e-07	PPR repeat
PPR_1	PF12854	491 - 523	3.3e-06	PPR repeat
PPR_2	PF13041	495 - 544	1.4e-13	PPR repeat family
PPR_3	PF13812	521 - 574	8.1e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	526 - 558	1.9e-06	PPR repeat
PPR_long	PF17177	550 - 663	2.6e-11	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	562 - 593	4.5e-08	PPR repeat
PPR_2	PF13041	565 - 613	3.3e-14	PPR repeat family
PPR	PF01535	569 - 598	1.9e-06	PPR repeat
PPR_1	PF12854	596 - 627	2.8e-06	PPR repeat
PPR_2	PF13041	608 - 649	2.6e-11	PPR repeat family
PPR_3	PF13812	624 - 680	1.1e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	631 - 664	2.7e-12	PPR repeat
PPR_2	PF13041	635 - 684	6.2e-19	PPR repeat family
PPR	PF01535	638 - 668	2.4e-07	PPR repeat
PPR_long	PF17177	652 - 770	1.1e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	659 - 717	6.6e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	666 - 696	9.9e-13	PPR repeat
PPR_2	PF13041	670 - 719	5.7e-15	PPR repeat family
PPR	PF01535	673 - 702	2e-06	PPR repeat
PPR_1	PF12854	701 - 734	1.5e-09	PPR repeat
PPR_2	PF13041	705 - 753	5.9e-13	PPR repeat family
PPR	PF01535	708 - 738	2.3e-07	PPR repeat
PPR_1	PF12854	737 - 767	7e-08	PPR repeat
PPR	PF01535	744 - 773	1.3e-06	PPR repeat
PPR_long	PF17177	755 - 896	8.8e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	764 - 819	6.3e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	776 - 823	4.9e-16	PPR repeat family
PPR_1	PF12854	778 - 803	1.4e-06	PPR repeat
PPR_long	PF17177	790 - 958	3.9e-08	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	806 - 837	1.1e-10	PPR repeat
PPR	PF01535	812 - 841	1.9e-08	PPR repeat
PPR_2	PF13041	814 - 856	4.3e-10	PPR repeat family
PPR_3	PF13812	833 - 891	1.8e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	875 - 906	2e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001276)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61990
fragaria_vesca	FvH4_5g09340 FvH4_5g09340 FvH4_5g09340 FvH4_5g09340
malus_domestica	MD06G1131900.v1.1 MD14G1148300.v1.1
prunus_persica	Prupe.5G142900_v2.0.a1 Prupe.5G142900_v2.0.a1
pyrus_communis	pycom06g12350 pycom14g12300
rosa_chinensis	RchiOBHm_Chr1g0325241 RchiOBHm_Chr1g0325261 RchiOBHm_Chr2g0162681 RchiOBHm_Chr7g0188811 RchiOBHm_Chr7g0229441
rosa_laevigata	RLG00000004657 RLG00000028121 RLG00000028122 RLG00000031426 RLG00000035825
rosa_multiflora	Rmu_sc0001621.1_g000013 Rmu_sc0001918.1_g000006 Rmu_sc0004279.1_g000018 Rmu_sc0034360.1_g000001 Rmu_sc0034361.1_g000001
rosa_roxburghii	Rroxscaffold_3G00266000 Rroxscaffold_5G00381140
rosa_rugosa	Rorug02G0360500 Rorug05G0369000 Rorug06G0494100 Rorug07G0156300
rosa_samantha	Rh1BG033100 Rh2CG348200 Rh2CG348300 Rh4AG001800 Rh4AG163800 Rh4BG001600 Rh4BG400000 Rh4BG400100 Rh4CG415000 Rh4CG415100 Rh6DG332400 Rh6DG332500 Rh7AG098900 Rh7AG408500 Rh7BG100600 Rh7CG102100 Rh7CG477400 Rh7DG100700 Rh7DG406300 Rh7DG406400 Rh7DG446700
rosa_wichuraiana	Rw0G022150 Rw7G008520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2255
Acc36I	ACCTGC	1 cut(s)	2255
Acc65I	GGTACC	1 cut(s)	293
AccB1I	GGYRCC	1 cut(s)	293
AccI	GTMKAC	1 cut(s)	899
AccII	CGCG	2 cut(s)	1652, 1788
AccIII	TCCGGA	1 cut(s)	1903
AciI	CCGC	2 cut(s)	1000, 1788
AclWI	GGATC	5 cut(s)	219, 1073, 1142, 1767, 1877
AcsI	RAATTY	6 cut(s)	316, 787, 1430, 1535, 1894, 2792
AcuI	CTGAAG	1 cut(s)	170
AfaI	GTAC	9 cut(s)	142, 295, 768, 796, 826, 1010, 1241, 1625, 2441
AfiI	CCNNNNNNNGG	4 cut(s)	217, 415, 894, 1351
AflIII	ACRYGT	1 cut(s)	2858
AhlI	ACTAGT	1 cut(s)	913
AjnI	CCWGG	3 cut(s)	246, 1350, 1838
AjuI	GAANNNNNNNTTGG	2 cut(s)	307, 339
Alw21I	GWGCWC	4 cut(s)	185, 312, 1884, 2847
Alw26I	GTCTC	4 cut(s)	1838, 1995, 2703, 2855
AlwI	GGATC	5 cut(s)	219, 1073, 1142, 1767, 1877
Ama87I	CYCGRG	1 cut(s)	1086
Aor13HI	TCCGGA	1 cut(s)	1903
AoxI	GGCC	6 cut(s)	1056, 1342, 1516, 2280, 2314, 2667
ApeKI	GCWGC	4 cut(s)	81, 533, 2321, 2912
ApoI	RAATTY	6 cut(s)	316, 787, 1430, 1535, 1894, 2792
AseI	ATTAAT	2 cut(s)	2037, 2982
Asp700I	GAANNNNTTC	1 cut(s)	1967
Asp718I	GGTACC	1 cut(s)	293
AspS9I	GGNCC	4 cut(s)	73, 405, 1057, 2314
AsuC2I	CCSGG	2 cut(s)	409, 1380
AsuHPI	GGTGA	5 cut(s)	266, 820, 1804, 2279, 2696
AvaI	CYCGRG	1 cut(s)	1086
AvaII	GGWCC	2 cut(s)	73, 405
AxyI	CCTNAGG	1 cut(s)	1061
BanI	GGYRCC	1 cut(s)	293
BanII	GRGCYC	2 cut(s)	185, 1884
BauI	CACGAG	1 cut(s)	213
Bbv12I	GWGCWC	4 cut(s)	185, 312, 1884, 2847
BbvI	GCAGC	4 cut(s)	68, 520, 2308, 2924
BceAI	ACGGC	1 cut(s)	2044
BciT130I	CCWGG	3 cut(s)	248, 1352, 1840
BclI	TGATCA	3 cut(s)	495, 705, 1359
BcnI	CCSGG	2 cut(s)	409, 1380
BcoDI	GTCTC	4 cut(s)	1838, 1995, 2703, 2855
BcuI	ACTAGT	1 cut(s)	913
BfaI	CTAG	3 cut(s)	914, 1676, 2708
BfmI	CTRYAG	2 cut(s)	82, 2525
BfuAI	ACCTGC	1 cut(s)	2255
BisI	GCNGC	4 cut(s)	82, 534, 2322, 2913
BlsI	GCNGC	4 cut(s)	83, 535, 2323, 2914
Bme1390I	CCNGG	5 cut(s)	248, 409, 1352, 1380, 1840
Bme18I	GGWCC	2 cut(s)	73, 405
BmeT110I	CYCGRG	1 cut(s)	1086
BmgT120I	GGNCC	4 cut(s)	73, 405, 1057, 2314
BmiI	GGNNCC	4 cut(s)	74, 295, 407, 1902
BmrFI	CCNGG	5 cut(s)	248, 409, 1352, 1380, 1840
BmrI	ACTGGG	1 cut(s)	1374
BmsI	GCATC	4 cut(s)	1662, 2077, 2187, 2749
BmuI	ACTGGG	1 cut(s)	1374
BplI	GAGNNNNNCTC	1 cut(s)	2993
BpmI	CTGGAG	1 cut(s)	1822
Bpu10I	CCTNAGC	1 cut(s)	2936
BpuEI	CTTGAG	1 cut(s)	2909
BpuMI	CCSGG	2 cut(s)	409, 1380
BsaAI	YACGTR	1 cut(s)	2439
BsaBI	GATNNNNATC	1 cut(s)	494
BsaI	GGTCTC	1 cut(s)	1838
BsaJI	CCNNGG	3 cut(s)	379, 1885, 2206
BsaWI	WCCGGW	1 cut(s)	1903
BsaXI	ACNNNNNCTCC	2 cut(s)	161, 191
Bsc4I	CCNNNNNNNGG	4 cut(s)	217, 415, 894, 1351
Bse118I	RCCGGY	1 cut(s)	415
Bse1I	ACTGG	2 cut(s)	1369, 2581
Bse21I	CCTNAGG	1 cut(s)	1061
Bse3DI	GCAATG	3 cut(s)	706, 1011, 1869
Bse8I	GATNNNNATC	1 cut(s)	494
BseAI	TCCGGA	1 cut(s)	1903
BseBI	CCWGG	3 cut(s)	248, 1352, 1840
BseDI	CCNNGG	3 cut(s)	379, 1885, 2206
BseGI	GGATG	8 cut(s)	121, 516, 633, 663, 736, 1912, 2719, 2951
BseJI	GATNNNNATC	1 cut(s)	494
BseLI	CCNNNNNNNGG	4 cut(s)	217, 415, 894, 1351
BseMI	GCAATG	3 cut(s)	706, 1011, 1869
BseMII	CTCAG	6 cut(s)	1052, 1869, 2006, 2163, 2860, 2950
BseNI	ACTGG	2 cut(s)	1369, 2581
BseRI	GAGGAG	2 cut(s)	1104, 1682
BseXI	GCAGC	4 cut(s)	68, 520, 2308, 2924
BsgI	GTGCAG	3 cut(s)	949, 1123, 2221
Bsh1236I	CGCG	2 cut(s)	1652, 1788
BshFI	GGCC	6 cut(s)	1058, 1344, 1518, 2282, 2316, 2669
BshNI	GGYRCC	1 cut(s)	293
BsiHKAI	GWGCWC	4 cut(s)	185, 312, 1884, 2847
BsiHKCI	CYCGRG	1 cut(s)	1086
BsiSI	CCGG	5 cut(s)	409, 416, 680, 1380, 1904
BslFI	GGGAC	3 cut(s)	1797, 2072, 2640
BslI	CCNNNNNNNGG	4 cut(s)	217, 415, 894, 1351
BsmAI	GTCTC	4 cut(s)	1838, 1995, 2703, 2855
BsmFI	GGGAC	3 cut(s)	1797, 2072, 2640
BsmI	GAATGC	4 cut(s)	322, 590, 1192, 2905
BsnI	GGCC	6 cut(s)	1058, 1344, 1518, 2282, 2316, 2669
Bso31I	GGTCTC	1 cut(s)	1838
BsoBI	CYCGRG	1 cut(s)	1086
Bsp1286I	GDGCHC	4 cut(s)	185, 312, 1884, 2847
Bsp13I	TCCGGA	1 cut(s)	1903
Bsp1407I	TGTACA	2 cut(s)	824, 1008
Bsp143I	GATC	9 cut(s)	224, 495, 705, 941, 1065, 1134, 1359, 1772, 1869
Bsp19I	CCATGG	1 cut(s)	2206
Bsp68I	TCGCGA	1 cut(s)	1652
BspACI	CCGC	2 cut(s)	1000, 1788
BspANI	GGCC	6 cut(s)	1058, 1344, 1518, 2282, 2316, 2669
BspCNI	CTCAG	6 cut(s)	1053, 1870, 2005, 2164, 2859, 2949
BspEI	TCCGGA	1 cut(s)	1903
BspFNI	CGCG	2 cut(s)	1652, 1788
BspLI	GGNNCC	4 cut(s)	74, 295, 407, 1902
BspMAI	CTGCAG	2 cut(s)	86, 2529
BspMI	ACCTGC	1 cut(s)	2255
BspPI	GGATC	5 cut(s)	219, 1073, 1142, 1767, 1877
BspQI	GCTCTTC	1 cut(s)	2487
BspT107I	GGYRCC	1 cut(s)	293
BspTNI	GGTCTC	1 cut(s)	1838
BsrDI	GCAATG	3 cut(s)	706, 1011, 1869
BsrFI	RCCGGY	1 cut(s)	415
BsrGI	TGTACA	2 cut(s)	824, 1008
BsrI	ACTGG	2 cut(s)	1369, 2581
BssAI	RCCGGY	1 cut(s)	415
BssECI	CCNNGG	3 cut(s)	379, 1885, 2206
BssMI	GATC	9 cut(s)	224, 495, 705, 941, 1065, 1134, 1359, 1772, 1869
BssNAI	GTATAC	1 cut(s)	900
BssSI	CACGAG	1 cut(s)	213
BssT1I	CCWWGG	2 cut(s)	1885, 2206
Bst1107I	GTATAC	1 cut(s)	900
Bst2BI	CACGAG	1 cut(s)	213
Bst2UI	CCWGG	3 cut(s)	248, 1352, 1840
Bst6I	CTCTTC	5 cut(s)	932, 1068, 1431, 2335, 2487
BstAUI	TGTACA	2 cut(s)	824, 1008
BstBAI	YACGTR	1 cut(s)	2439
BstC8I	GCNNGC	6 cut(s)	375, 417, 1946, 2529, 2933, 3007
BstDEI	CTNAG	7 cut(s)	669, 1061, 1878, 1992, 2172, 2846, 2936
BstDSI	CCRYGG	2 cut(s)	379, 2206
BstEII	GGTNACC	2 cut(s)	254, 1792
BstF5I	GGATG	8 cut(s)	121, 516, 633, 663, 736, 1912, 2719, 2951
BstFNI	CGCG	2 cut(s)	1652, 1788
BstKTI	GATC	9 cut(s)	227, 498, 708, 944, 1068, 1137, 1362, 1775, 1872
BstMAI	GTCTC	4 cut(s)	1838, 1995, 2703, 2855
BstMBI	GATC	9 cut(s)	224, 495, 705, 941, 1065, 1134, 1359, 1772, 1869
BstMWI	GCNNNNNNNGC	5 cut(s)	370, 2472, 2571, 2580, 2909
BstNI	CCWGG	3 cut(s)	248, 1352, 1840
BstNSI	RCATGY	4 cut(s)	377, 2420, 2862, 2935
BstPI	GGTNACC	2 cut(s)	254, 1792
BstSCI	CCNGG	5 cut(s)	246, 407, 1350, 1378, 1838
BstSFI	CTRYAG	2 cut(s)	82, 2525
BstSNI	TACGTA	1 cut(s)	2439
BstUI	CGCG	2 cut(s)	1652, 1788
BstV1I	GCAGC	4 cut(s)	68, 520, 2308, 2924
BstX2I	RGATCY	3 cut(s)	1134, 1772, 1869
BstXI	CCANNNNNNTGG	1 cut(s)	251
BstYI	RGATCY	3 cut(s)	1134, 1772, 1869
BstZ17I	GTATAC	1 cut(s)	900
Bsu36I	CCTNAGG	1 cut(s)	1061
BsuRI	GGCC	6 cut(s)	1058, 1344, 1518, 2282, 2316, 2669
BtgI	CCRYGG	2 cut(s)	379, 2206
BtsCI	GGATG	8 cut(s)	121, 516, 633, 663, 736, 1912, 2719, 2951
BtsI	GCAGTG	3 cut(s)	76, 1111, 1729
BtsIMutI	CAGTG	8 cut(s)	76, 132, 1111, 1362, 1729, 1822, 2574, 2752
BtuMI	TCGCGA	1 cut(s)	1652
BveI	ACCTGC	1 cut(s)	2255
Cac8I	GCNNGC	6 cut(s)	375, 417, 1946, 2529, 2933, 3007
Cfr10I	RCCGGY	1 cut(s)	415
Cfr13I	GGNCC	4 cut(s)	73, 405, 1057, 2314
CseI	GACGC	1 cut(s)	1794
CsiI	ACCWGGT	1 cut(s)	246
Csp6I	GTAC	9 cut(s)	141, 294, 767, 795, 825, 1009, 1240, 1624, 2440
CviQI	GTAC	9 cut(s)	141, 294, 767, 795, 825, 1009, 1240, 1624, 2440
DdeI	CTNAG	7 cut(s)	669, 1061, 1878, 1992, 2172, 2846, 2936
DpnI	GATC	9 cut(s)	226, 497, 707, 943, 1067, 1136, 1361, 1774, 1871
DpnII	GATC	9 cut(s)	224, 495, 705, 941, 1065, 1134, 1359, 1772, 1869
Eam1104I	CTCTTC	5 cut(s)	932, 1068, 1431, 2335, 2487
EarI	CTCTTC	5 cut(s)	932, 1068, 1431, 2335, 2487
Ecl136II	GAGCTC	2 cut(s)	183, 1882
Eco105I	TACGTA	1 cut(s)	2439
Eco130I	CCWWGG	2 cut(s)	1885, 2206
Eco147I	AGGCCT	2 cut(s)	2282, 2669
Eco24I	GRGCYC	2 cut(s)	185, 1884
Eco31I	GGTCTC	1 cut(s)	1838
Eco32I	GATATC	1 cut(s)	1804
Eco47I	GGWCC	2 cut(s)	73, 405
Eco53kI	GAGCTC	2 cut(s)	183, 1882
Eco57I	CTGAAG	1 cut(s)	170
Eco81I	CCTNAGG	1 cut(s)	1061
Eco88I	CYCGRG	1 cut(s)	1086
Eco91I	GGTNACC	2 cut(s)	254, 1792
EcoICRI	GAGCTC	2 cut(s)	183, 1882
EcoO109I	RGGNCCY	3 cut(s)	405, 1057, 2314
EcoO65I	GGTNACC	2 cut(s)	254, 1792
EcoRII	CCWGG	3 cut(s)	246, 1350, 1838
EcoRV	GATATC	1 cut(s)	1804
EcoT14I	CCWWGG	2 cut(s)	1885, 2206
EcoT22I	ATGCAT	3 cut(s)	1276, 1597, 2344
EcoT38I	GRGCYC	2 cut(s)	185, 1884
ErhI	CCWWGG	2 cut(s)	1885, 2206
FaqI	GGGAC	3 cut(s)	1797, 2072, 2640
FauI	CCCGC	1 cut(s)	1781
FbaI	TGATCA	3 cut(s)	495, 705, 1359
FblI	GTMKAC	1 cut(s)	899
Fnu4HI	GCNGC	4 cut(s)	82, 534, 2322, 2913
FokI	GGATG	8 cut(s)	128, 523, 640, 670, 743, 1919, 2726, 2958
FriOI	GRGCYC	2 cut(s)	185, 1884
Fsp4HI	GCNGC	4 cut(s)	82, 534, 2322, 2913
FspBI	CTAG	3 cut(s)	914, 1676, 2708
GluI	GCNGC	4 cut(s)	82, 534, 2322, 2913
GsuI	CTGGAG	1 cut(s)	1822
HaeIII	GGCC	6 cut(s)	1058, 1344, 1518, 2282, 2316, 2669
HapII	CCGG	5 cut(s)	409, 416, 680, 1380, 1904
HgaI	GACGC	1 cut(s)	1794
HindIII	AAGCTT	1 cut(s)	362
HpaII	CCGG	5 cut(s)	409, 416, 680, 1380, 1904
HphI	GGTGA	5 cut(s)	266, 820, 1804, 2279, 2696
Hpy166II	GTNNAC	9 cut(s)	76, 825, 900, 1261, 1618, 2108, 2442, 2875, 2961
Hpy8I	GTNNAC	9 cut(s)	76, 825, 900, 1261, 1618, 2108, 2442, 2875, 2961
HpyAV	CCTTC	8 cut(s)	610, 766, 808, 871, 1153, 1450, 1718, 2675
HpyCH4IV	ACGT	2 cut(s)	1047, 2438
HpyF10VI	GCNNNNNNNGC	5 cut(s)	370, 2472, 2571, 2580, 2909
HpyF3I	CTNAG	7 cut(s)	669, 1061, 1878, 1992, 2172, 2846, 2936
HpySE526I	ACGT	2 cut(s)	1047, 2438
Kpn2I	TCCGGA	1 cut(s)	1903
KpnI	GGTACC	1 cut(s)	297
KroI	GCCGGC	1 cut(s)	415
KroNI	GCCGGC	1 cut(s)	417
Ksp22I	TGATCA	3 cut(s)	495, 705, 1359
Kzo9I	GATC	9 cut(s)	224, 495, 705, 941, 1065, 1134, 1359, 1772, 1869
LguI	GCTCTTC	1 cut(s)	2487
LmnI	GCTCC	4 cut(s)	315, 1249, 1696, 1887
Lsp1109I	GCAGC	4 cut(s)	68, 520, 2308, 2924
LweI	GCATC	4 cut(s)	1662, 2077, 2187, 2749
MabI	ACCWGGT	1 cut(s)	246
MaeI	CTAG	3 cut(s)	914, 1676, 2708
MaeII	ACGT	2 cut(s)	1047, 2438
MalI	GATC	9 cut(s)	226, 497, 707, 943, 1067, 1136, 1361, 1774, 1871
MboI	GATC	9 cut(s)	224, 495, 705, 941, 1065, 1134, 1359, 1772, 1869
MfeI	CAATTG	1 cut(s)	299
MflI	RGATCY	3 cut(s)	1134, 1772, 1869
MhlI	GDGCHC	4 cut(s)	185, 312, 1884, 2847
MlyI	GAGTC	3 cut(s)	179, 2622, 2966
MmeI	TCCRAC	2 cut(s)	369, 1996
Mph1103I	ATGCAT	3 cut(s)	1276, 1597, 2344
MroI	TCCGGA	1 cut(s)	1903
MroNI	GCCGGC	1 cut(s)	415
MroXI	GAANNNNTTC	1 cut(s)	1967
MslI	CAYNNNNRTG	1 cut(s)	378
MspI	CCGG	5 cut(s)	409, 416, 680, 1380, 1904
MspR9I	CCNGG	5 cut(s)	248, 409, 1352, 1380, 1840
MunI	CAATTG	1 cut(s)	299
Mva1269I	GAATGC	4 cut(s)	322, 590, 1192, 2905
MvaI	CCWGG	3 cut(s)	248, 1352, 1840
MvnI	CGCG	2 cut(s)	1652, 1788
MwoI	GCNNNNNNNGC	5 cut(s)	370, 2472, 2571, 2580, 2909
NaeI	GCCGGC	1 cut(s)	417
NciI	CCSGG	2 cut(s)	409, 1380
NcoI	CCATGG	1 cut(s)	2206
NdeII	GATC	9 cut(s)	224, 495, 705, 941, 1065, 1134, 1359, 1772, 1869
NgoMIV	GCCGGC	1 cut(s)	415
NlaIV	GGNNCC	4 cut(s)	74, 295, 407, 1902
NmuCI	GTSAC	9 cut(s)	254, 605, 1108, 1318, 1792, 2017, 2122, 2419, 2811
NruI	TCGCGA	1 cut(s)	1652
NsiI	ATGCAT	3 cut(s)	1276, 1597, 2344
NspI	RCATGY	4 cut(s)	377, 2420, 2862, 2935
PacI	TTAATTAA	1 cut(s)	2982
PaeI	GCATGC	2 cut(s)	377, 2935
PaeR7I	CTCGAG	1 cut(s)	1086
PaqCI	CACCTGC	1 cut(s)	2255
PceI	AGGCCT	2 cut(s)	2282, 2669
PciI	ACATGT	1 cut(s)	2858
PciSI	GCTCTTC	1 cut(s)	2487
PcsI	WCGNNNNNNNCGW	1 cut(s)	220
PctI	GAATGC	4 cut(s)	322, 590, 1192, 2905
PdiI	GCCGGC	1 cut(s)	417
PdmI	GAANNNNTTC	1 cut(s)	1967
PfoI	TCCNGGA	1 cut(s)	1838
PkrI	GCNGC	4 cut(s)	83, 535, 2323, 2914
PleI	GAGTC	3 cut(s)	178, 2622, 2965
PpsI	GAGTC	3 cut(s)	178, 2622, 2965
Ppu21I	YACGTR	1 cut(s)	2439
PpuMI	RGGWCCY	1 cut(s)	405
PscI	ACATGT	1 cut(s)	2858
PshBI	ATTAAT	2 cut(s)	2037, 2982
Psp124BI	GAGCTC	2 cut(s)	185, 1884
Psp5II	RGGWCCY	1 cut(s)	405
Psp6I	CCWGG	3 cut(s)	246, 1350, 1838
PspEI	GGTNACC	2 cut(s)	254, 1792
PspGI	CCWGG	3 cut(s)	246, 1350, 1838
PspN4I	GGNNCC	4 cut(s)	74, 295, 407, 1902
PspPI	GGNCC	4 cut(s)	73, 405, 1057, 2314
PspPPI	RGGWCCY	1 cut(s)	405
PspXI	VCTCGAGB	1 cut(s)	1086
PstI	CTGCAG	2 cut(s)	86, 2529
PsuI	RGATCY	3 cut(s)	1134, 1772, 1869
RruI	TCGCGA	1 cut(s)	1652
RsaI	GTAC	9 cut(s)	142, 295, 768, 796, 826, 1010, 1241, 1625, 2441
RsaNI	GTAC	9 cut(s)	141, 294, 767, 795, 825, 1009, 1240, 1624, 2440
RseI	CAYNNNNRTG	1 cut(s)	378
SacI	GAGCTC	2 cut(s)	185, 1884
SapI	GCTCTTC	1 cut(s)	2487
SatI	GCNGC	4 cut(s)	82, 534, 2322, 2913
Sau3AI	GATC	9 cut(s)	224, 495, 705, 941, 1065, 1134, 1359, 1772, 1869
Sau96I	GGNCC	4 cut(s)	73, 405, 1057, 2314
SchI	GAGTC	3 cut(s)	179, 2622, 2966
ScrFI	CCNGG	5 cut(s)	248, 409, 1352, 1380, 1840
SduI	GDGCHC	4 cut(s)	185, 312, 1884, 2847
SexAI	ACCWGGT	1 cut(s)	246
SfaNI	GCATC	4 cut(s)	1662, 2077, 2187, 2749
SfcI	CTRYAG	2 cut(s)	82, 2525
Sfr274I	CTCGAG	1 cut(s)	1086
SinI	GGWCC	2 cut(s)	73, 405
SlaI	CTCGAG	1 cut(s)	1086
SmiMI	CAYNNNNRTG	1 cut(s)	378
SmlI	CTYRAG	2 cut(s)	1086, 2924
SmoI	CTYRAG	2 cut(s)	1086, 2924
SnaBI	TACGTA	1 cut(s)	2439
SpeI	ACTAGT	1 cut(s)	913
SphI	GCATGC	2 cut(s)	377, 2935
SseBI	AGGCCT	2 cut(s)	2282, 2669
SsiI	CCGC	2 cut(s)	1000, 1788
SspI	AATATT	1 cut(s)	2536
SspMI	CTAG	3 cut(s)	914, 1676, 2708
SstI	GAGCTC	2 cut(s)	185, 1884
StuI	AGGCCT	2 cut(s)	2282, 2669
StyD4I	CCNGG	5 cut(s)	246, 407, 1350, 1378, 1838
StyI	CCWWGG	2 cut(s)	1885, 2206
TaiI	ACGT	2 cut(s)	1050, 2441
TaqI	TCGA	5 cut(s)	223, 332, 1087, 1665, 2664
TatI	WGTACW	2 cut(s)	824, 1008
TscAI	CASTG	8 cut(s)	83, 132, 1111, 1369, 1736, 1822, 2581, 2752
TseFI	GTSAC	9 cut(s)	254, 605, 1108, 1318, 1792, 2017, 2122, 2419, 2811
TseI	GCWGC	4 cut(s)	81, 533, 2321, 2912
Tsp45I	GTSAC	9 cut(s)	254, 605, 1108, 1318, 1792, 2017, 2122, 2419, 2811
TspGWI	ACGGA	4 cut(s)	163, 217, 2160, 2892
TspRI	CASTG	8 cut(s)	83, 132, 1111, 1369, 1736, 1822, 2581, 2752
VpaK11BI	GGWCC	2 cut(s)	73, 405
VspI	ATTAAT	2 cut(s)	2037, 2982
XapI	RAATTY	6 cut(s)	316, 787, 1430, 1535, 1894, 2792
XceI	RCATGY	4 cut(s)	377, 2420, 2862, 2935
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1555
XhoI	CTCGAG	1 cut(s)	1086
XmiI	GTMKAC	1 cut(s)	899
XmnI	GAANNNNTTC	1 cut(s)	1967
XspI	CTAG	3 cut(s)	914, 1676, 2708
Zsp2I	ATGCAT	3 cut(s)	1276, 1597, 2344

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.