Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0001276

Physical Location & Seq

Genomic Coordinates Forward (+)

61212616 .. 61216315

Gene Structure 3700 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004657

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3060 bp

ATGGGTTTAGCTTCACATGGGAGAAACCTTGCATTTCAGATTGGTAATCCAAAATCAAGGCTTTTGATTCGGGTCCACTGCTGCAGTTATAAATCAGAGAGTAAAAAGCAAGAGGATGATGAAACAGTGAGGGAAATCTGTACCATTCTGAAGCGAAGCAGAGAGTGGGAGTCCGTGTTGAGCTCATCAGGGTTTCCCAAGAAGCTAAACCCTCGTGTGGTTCGATCCGTTCTTCAACAACACCACCAGGTTGGTGACCCAGAAAGACTTTTAAGTTTCTTTGATTGGTCTGGTGCCCAATTGGGTGTGCTCCCAAATTTGCATTCTTTTTCGATTATGGCTGTTGTTTTGTGTAATTCCAAGCTTTTTGGGCATGCCCATGGTGTGTTGGAGAGAATGGTTAGGACCCGGAAGCCGGCTTTAGAAGTTTTGGATTGTGTTGTTAGGTGTTTTAGAGAGTTTGAGGGGTCTGATATGGTGGTTTTTGAGATTTTGATCAATGTGTTTAGGATGGGGGGTTGGTTTTACGAGGCTGCTGATGTGTTTTTGGGTGTTAGGAGTGTTGGGATTTCGCTGAGATTGGAATGCTGTAATTCTCTGTTGAATGAGTTGTTGAAGGGTAATAGGATGAGGCTGTTCTGGAAAGTGTATGATGGGATGTTAGAGGCGAAGATTGAGCCAGATTTTTACACTTATAGCAATGTGATCAACGCACATTGTAAGTTTGGGGATGTTAGGCAGGGTAAGAGGCTACTGTTTGAGATGGTAGAGAAGGGCTGTCATCCGAATTTGAGTACCTTCAATGTGGTGATTGATGGATTGTGTAGAAGTAGGGCTGTTGATGAAGCCATTGAGTTGAAAAAATCGATGGTTGAGAAGGGATTGACACCTGATACGTATACATATTCAGTACTAATTGATGGATTGTGCAGACAGAAGAGATCAGAAGAAGCAAAGCTGGTATTGAACGAGATGTATGATATAGGTTTGAATCCTGACCGCACCTTGTACGTTGCTTTGATTGATGGATTCTTGAAAGAAAGTAATGTGGATAAGGCCCTGAGGATCAAAGAAGAGATGGTCACTCGAGGAGTTAAGTTGTGCGGTGTCACATATAATGTGATTTTTGCTGGGATCTGTAAAATAGGTAAGATGGAGAAGGCAGAATCTCTTTCGAATGAGATGAATGCTATGGGAATTGAACCTAATACCCAGACATATAACTATTTAATTGATGGGTACTGTCGGGAGCAAAATGTAAACAAGGCTTATGCATTACTTAATGAGATGAAGCAAAGGAACTTGACGCCCAATGAGGTCACTTGTGGAGTGGTAATCAATGGCCTATGCCGGTCTGGAGATCTACAAGGGGCTAATAATGTTTTGAAGGTAATGATCACTGGGGGTTTGAAACCCGGTAGAGTTATCTACACAACTCTAATAAAAGGTCATCTACAAGAAAGAAAATTTGAAGATGCGATAAAACTATTGAAGGAAATGAGTGAAAAAGGTGTCATTCCTGATGTCTTTTGCTACAATTCTCTTGTAATTGGCCTGTGCAAAGCGAGGAAATTTGAAGAAGCCAACACTTATTTGGTGGAAATGGTTGAGAGAGGATTAAAGCCTAATGCATATACTTATGGGGCTTTTGTTCACGGGTACTGTAAGGAGAGGGAAATGCAACTTGCGAATAGGTATTTTGAGGAGATGCTAGGTTCTGGTATAGCTCCCAATGAGTTTATCTATACAGCCCTGATTGAAGGACACTGCAAAGAGGGTAACTTGGTTGAAGCTCTTTCAACATTTAGATCCATGTTGGGACGCGGGGTGACCCCAGATATCAAAACATACAGTGTCATTATTCATGGTCTCTCCAGGAGAGGAAAACTTGAAGAAGCAATGGGGATCTTCTGTGAGCTCCTTGGCAAGAATTTGGTTCCGGATGTATTTACATACAGCTCTCTCATATCTGGCTTTTGCAAGCAGGGAAATGCAGAGAAGTCTTTCCAACTTCTTGAACAGATGTCTCAGAGAGGTATTAAACCAAACATTGTCACTTACAACGGCTTGATTAATGGATTGTGCAAGTCTGGGGACATTGACAGAGCCAGAGAAGTGTTTGATGCAATTTCAGGAAAGGGTTTAACTCCTAATGCTGTGACTTATGCTACAATGATGGACGGATATAGCAAATCTGGAAAATTAACTGAGGCTTTACAATTATTAGATGAGATGCAACTCCATGGGATTCCTACTGATAGTTTCATTTACTGCACCCTGATTGATGGGTTCTGCATGGCAGGTGATACAGACAAGGCCTTGTCTTTATTTAATGAAGTGGTGGATAAAGGCCTTGCTGCCACTTCTTCTTTCAATGCATTGATTGATGGCTTCTGTAAATTGGGGAAGCAGATGGAAGCCAAACAGTTGTTTGAAGATATGGTGGATAAGCATGTGACACCAAATCATGTTACGTACACGATTCTGATTGATTCTCTCTGCAAAGAGGGCTTGTTGAGGGAATCAGAAGAGCTATTTCTGGAAATGCAGGAAAGGAATCTGCAGGCCAATATTGTAACCTACACTTCACTTTTGCAGGGCTACAACAGCACAGGAAGCAGAGTAAAGATGTTTGCTCTTTACGAGGAAATGATAGCAAGCGGACTCAAACCTGATGAAATAACCTATGGTCTGATGGTCGAGGCCTATTGCGAAGAAGGTGATGTGGTAAAGTCTCTAAAGCTGGTGGATGAAAGTTTGGTCAATAGTGTTAATTTGAACAGTGATGTGGTAGATGCTCTGGCAAATGCCCTATTCCAAAAGGCAGAATTTTCGAAAGTGATGAAGTCACTTGATGAAATGGGAGAGCAAGGATTTGTGCTCAGTCTTGCTACATGTAAAACATTAGTTCACGGATTTTTCTACAAATCAGGGAATGCAGAGAGGGCAGCAAGGATTCTCGAGAGCATGCTCAGGTTTGGATGGGTTTCAGAGTCCACAAGTATAAGCGACTTAATTAATGATGAAGACCAAAATGAGGCAAGCTCTGGGATTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00260 ko00261 ko00270 ko00300 ko01100 ko01110 ko01120 ko01130 ko01210 ko01230 map00260 map00261 map00270 map00300 map01100 map01110 map01120 map01130 map01210 map01230

Pfam Domains

Protein Families

Mem_trans PPR PPR_1 PPR_2 PPR_3 PPR_long

Protein Analysis

1020

Amino Acids

114.01

Weight (kDa)

5.88

Isoelectric Point (pI)

27.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	196 - 241	2.6e-09	PPR repeat family
PPR_long	PF17177	197 - 306	9.3e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	217 - 272	9e-07	Pentatricopeptide repeat domain
PPR	PF01535	230 - 260	9.8e-06	PPR repeat
PPR_2	PF13041	232 - 276	4.3e-11	PPR repeat family
PPR_1	PF12854	258 - 291	2.2e-09	PPR repeat
PPR_2	PF13041	262 - 311	3.2e-17	PPR repeat family
PPR_1	PF12854	293 - 325	8.4e-10	PPR repeat
PPR_3	PF13812	355 - 414	3.2e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	370 - 416	1.2e-15	PPR repeat family
PPR_1	PF12854	398 - 431	3.5e-12	PPR repeat
PPR_2	PF13041	403 - 451	6e-17	PPR repeat family
PPR	PF01535	406 - 434	8.9e-07	PPR repeat
PPR_3	PF13812	469 - 520	7.2e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	472 - 521	1.2e-14	PPR repeat family
PPR	PF01535	475 - 505	1.4e-06	PPR repeat
PPR_1	PF12854	503 - 535	3.3e-06	PPR repeat
PPR_2	PF13041	507 - 556	1.4e-13	PPR repeat family
PPR_3	PF13812	533 - 586	8.2e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	538 - 570	1.9e-06	PPR repeat
PPR_long	PF17177	563 - 673	1.2e-09	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	574 - 605	4.5e-08	PPR repeat
PPR_2	PF13041	577 - 625	3.3e-14	PPR repeat family
PPR	PF01535	581 - 610	1.9e-06	PPR repeat
PPR_1	PF12854	608 - 639	4.7e-06	PPR repeat
PPR_2	PF13041	620 - 661	3.3e-11	PPR repeat family
PPR_long	PF17177	631 - 743	6.2e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	637 - 692	2e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	643 - 676	2.7e-12	PPR repeat
PPR	PF01535	650 - 680	2.4e-07	PPR repeat
PPR_2	PF13041	654 - 696	8.3e-15	PPR repeat family
PPR_long	PF17177	664 - 781	2.6e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	671 - 729	1.4e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	678 - 708	1e-12	PPR repeat
PPR_2	PF13041	682 - 731	8.2e-15	PPR repeat family
PPR	PF01535	685 - 714	2e-06	PPR repeat
PPR_1	PF12854	713 - 746	5.4e-10	PPR repeat
PPR_2	PF13041	717 - 765	1.8e-13	PPR repeat family
PPR	PF01535	720 - 750	9.2e-08	PPR repeat
PPR_1	PF12854	749 - 779	7.1e-08	PPR repeat
PPR	PF01535	756 - 785	1.3e-06	PPR repeat
PPR_3	PF13812	776 - 831	6.4e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	788 - 835	5e-16	PPR repeat family
PPR_1	PF12854	790 - 815	1.5e-06	PPR repeat
PPR_long	PF17177	805 - 969	1.5e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	818 - 849	1.1e-10	PPR repeat
PPR	PF01535	824 - 853	2e-08	PPR repeat
PPR_2	PF13041	824 - 868	3.1e-12	PPR repeat family
PPR_3	PF13812	845 - 903	4.8e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	857 - 904	3.1e-10	PPR repeat family
PPR_1	PF12854	888 - 918	3.2e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001276)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61990
fragaria_vesca	FvH4_5g09340 FvH4_5g09340 FvH4_5g09340 FvH4_5g09340
malus_domestica	MD06G1131900.v1.1 MD14G1148300.v1.1
prunus_persica	Prupe.5G142900_v2.0.a1 Prupe.5G142900_v2.0.a1
pyrus_communis	pycom06g12350 pycom14g12300
rosa_chinensis	RchiOBHm_Chr1g0325241 RchiOBHm_Chr1g0325261 RchiOBHm_Chr2g0162681 RchiOBHm_Chr7g0188811 RchiOBHm_Chr7g0229441
rosa_laevigata	RLG00000004657 RLG00000028121 RLG00000028122 RLG00000031426 RLG00000035825
rosa_multiflora	Rmu_sc0001621.1_g000013 Rmu_sc0001918.1_g000006 Rmu_sc0004279.1_g000018 Rmu_sc0034360.1_g000001 Rmu_sc0034361.1_g000001
rosa_roxburghii	Rroxscaffold_3G00266000 Rroxscaffold_5G00381140
rosa_rugosa	Rorug02G0360500 Rorug05G0369000 Rorug06G0494100 Rorug07G0156300
rosa_samantha	Rh1BG033100 Rh2CG348200 Rh2CG348300 Rh4AG001800 Rh4AG163800 Rh4BG001600 Rh4BG400000 Rh4BG400100 Rh4CG415000 Rh4CG415100 Rh6DG332400 Rh6DG332500 Rh7AG098900 Rh7AG408500 Rh7BG100600 Rh7CG102100 Rh7CG477400 Rh7DG100700 Rh7DG406300 Rh7DG406400 Rh7DG446700
rosa_wichuraiana	Rw0G022150 Rw7G008520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	90
AarI	CACCTGC	1 cut(s)	2291
Acc36I	ACCTGC	1 cut(s)	2291
AccB1I	GGYRCC	1 cut(s)	293
AccI	GTMKAC	1 cut(s)	899
AccII	CGCG	1 cut(s)	1824
AccIII	TCCGGA	1 cut(s)	1939
AciI	CCGC	4 cut(s)	1000, 1104, 1824, 2661
AclWI	GGATC	5 cut(s)	219, 1073, 1142, 1803, 1913
AcsI	RAATTY	6 cut(s)	316, 787, 1466, 1571, 1930, 2828
AcuI	CTGAAG	1 cut(s)	170
AcyI	GRCGYC	1 cut(s)	1307
AfaI	GTAC	7 cut(s)	142, 796, 912, 1010, 1241, 1661, 2477
AfiI	CCNNNNNNNGG	3 cut(s)	217, 415, 3037
AflIII	ACRYGT	1 cut(s)	2894
AjnI	CCWGG	2 cut(s)	246, 1874
AjuI	GAANNNNNNNTTGG	2 cut(s)	307, 339
Alw21I	GWGCWC	4 cut(s)	185, 312, 1920, 2883
Alw26I	GTCTC	3 cut(s)	1874, 2031, 2739
AlwI	GGATC	5 cut(s)	219, 1073, 1142, 1803, 1913
Ama87I	CYCGRG	2 cut(s)	1086, 2960
Aor13HI	TCCGGA	1 cut(s)	1939
AoxI	GGCC	7 cut(s)	1056, 1342, 1552, 2316, 2350, 2565, 2703
ApeKI	GCWGC	4 cut(s)	81, 533, 2357, 2948
ApoI	RAATTY	6 cut(s)	316, 787, 1466, 1571, 1930, 2828
AseI	ATTAAT	2 cut(s)	2073, 3018
Asp700I	GAANNNNTTC	1 cut(s)	2003
AspS9I	GGNCC	3 cut(s)	73, 405, 1057
AsuC2I	CCSGG	2 cut(s)	409, 1416
AsuHPI	GGTGA	5 cut(s)	266, 820, 1840, 2315, 2732
AsuII	TTCGAA	2 cut(s)	1175, 2834
AvaI	CYCGRG	2 cut(s)	1086, 2960
AvaII	GGWCC	2 cut(s)	73, 405
AxyI	CCTNAGG	1 cut(s)	1061
BaeGI	GKGCMC	1 cut(s)	298
BanI	GGYRCC	1 cut(s)	293
BanII	GRGCYC	2 cut(s)	185, 1920
BauI	CACGAG	1 cut(s)	213
BbsI	GAAGAC	1 cut(s)	3033
Bbv12I	GWGCWC	4 cut(s)	185, 312, 1920, 2883
BbvI	GCAGC	4 cut(s)	68, 520, 2344, 2960
BceAI	ACGGC	1 cut(s)	2080
BcgI	CGANNNNNNTGC	2 cut(s)	2814, 2848
BciT130I	CCWGG	2 cut(s)	248, 1876
BclI	TGATCA	3 cut(s)	495, 705, 1395
BcnI	CCSGG	2 cut(s)	409, 1416
BcoDI	GTCTC	3 cut(s)	1874, 2031, 2739
BfaI	CTAG	1 cut(s)	1712
BfmI	CTRYAG	2 cut(s)	82, 2561
BfuAI	ACCTGC	1 cut(s)	2291
BglII	AGATCT	1 cut(s)	1360
BisI	GCNGC	4 cut(s)	82, 534, 2358, 2949
BlsI	GCNGC	4 cut(s)	83, 535, 2359, 2950
BmcAI	AGTACT	1 cut(s)	912
Bme1390I	CCNGG	4 cut(s)	248, 409, 1416, 1876
Bme18I	GGWCC	2 cut(s)	73, 405
BmeT110I	CYCGRG	2 cut(s)	1086, 2960
BmgT120I	GGNCC	3 cut(s)	73, 405, 1057
BmiI	GGNNCC	4 cut(s)	74, 295, 407, 1938
BmrFI	CCNGG	4 cut(s)	248, 409, 1416, 1876
BmrI	ACTGGG	1 cut(s)	1410
BmsI	GCATC	5 cut(s)	1465, 1698, 2113, 2223, 2785
BmuI	ACTGGG	1 cut(s)	1410
BpiI	GAAGAC	1 cut(s)	3033
BplI	GAGNNNNNCTC	1 cut(s)	3029
BpmI	CTGGAG	2 cut(s)	1377, 1858
Bpu10I	CCTNAGC	1 cut(s)	2972
Bpu14I	TTCGAA	2 cut(s)	1175, 2834
BpuMI	CCSGG	2 cut(s)	409, 1416
Bsa29I	ATCGAT	1 cut(s)	866
BsaAI	YACGTR	2 cut(s)	897, 2475
BsaBI	GATNNNNATC	1 cut(s)	494
BsaHI	GRCGYC	1 cut(s)	1307
BsaI	GGTCTC	1 cut(s)	1874
BsaJI	CCNNGG	3 cut(s)	379, 1921, 2242
BsaWI	WCCGGW	1 cut(s)	1939
BsaXI	ACNNNNNCTCC	2 cut(s)	161, 191
Bsc4I	CCNNNNNNNGG	3 cut(s)	217, 415, 3037
Bse118I	RCCGGY	2 cut(s)	415, 1350
Bse1I	ACTGG	1 cut(s)	1405
Bse21I	CCTNAGG	1 cut(s)	1061
Bse3DI	GCAATG	2 cut(s)	706, 1905
Bse8I	GATNNNNATC	1 cut(s)	494
BseAI	TCCGGA	1 cut(s)	1939
BseBI	CCWGG	2 cut(s)	248, 1876
BseCI	ATCGAT	1 cut(s)	866
BseDI	CCNNGG	3 cut(s)	379, 1921, 2242
BseGI	GGATG	9 cut(s)	121, 516, 633, 663, 736, 781, 1948, 2755, 2987
BseJI	GATNNNNATC	1 cut(s)	494
BseLI	CCNNNNNNNGG	3 cut(s)	217, 415, 3037
BseMI	GCAATG	2 cut(s)	706, 1905
BseMII	CTCAG	6 cut(s)	566, 1052, 2042, 2199, 2896, 2986
BseNI	ACTGG	1 cut(s)	1405
BseRI	GAGGAG	2 cut(s)	1104, 1718
BseSI	GKGCMC	1 cut(s)	298
BseXI	GCAGC	4 cut(s)	68, 520, 2344, 2960
BseYI	CCCAGC	1 cut(s)	1130
BsgI	GTGCAG	2 cut(s)	949, 2257
Bsh1236I	CGCG	1 cut(s)	1824
BshFI	GGCC	7 cut(s)	1058, 1344, 1554, 2318, 2352, 2567, 2705
BshNI	GGYRCC	1 cut(s)	293
BshVI	ATCGAT	1 cut(s)	866
BsiHKAI	GWGCWC	4 cut(s)	185, 312, 1920, 2883
BsiHKCI	CYCGRG	2 cut(s)	1086, 2960
BsiSI	CCGG	5 cut(s)	409, 416, 1351, 1416, 1940
BslFI	GGGAC	2 cut(s)	1833, 2108
BslI	CCNNNNNNNGG	3 cut(s)	217, 415, 3037
BsmAI	GTCTC	3 cut(s)	1874, 2031, 2739
BsmFI	GGGAC	2 cut(s)	1833, 2108
BsmI	GAATGC	4 cut(s)	322, 590, 1192, 2941
BsnI	GGCC	7 cut(s)	1058, 1344, 1554, 2318, 2352, 2567, 2705
Bso31I	GGTCTC	1 cut(s)	1874
BsoBI	CYCGRG	2 cut(s)	1086, 2960
Bsp119I	TTCGAA	2 cut(s)	1175, 2834
Bsp1286I	GDGCHC	5 cut(s)	185, 298, 312, 1920, 2883
Bsp13I	TCCGGA	1 cut(s)	1939
Bsp19I	CCATGG	2 cut(s)	379, 2242
BspACI	CCGC	4 cut(s)	1000, 1104, 1824, 2661
BspANI	GGCC	7 cut(s)	1058, 1344, 1554, 2318, 2352, 2567, 2705
BspCNI	CTCAG	6 cut(s)	567, 1053, 2041, 2200, 2895, 2985
BspDI	ATCGAT	1 cut(s)	866
BspEI	TCCGGA	1 cut(s)	1939
BspFNI	CGCG	1 cut(s)	1824
BspLI	GGNNCC	4 cut(s)	74, 295, 407, 1938
BspMAI	CTGCAG	2 cut(s)	86, 2565
BspMI	ACCTGC	1 cut(s)	2291
BspPI	GGATC	5 cut(s)	219, 1073, 1142, 1803, 1913
BspQI	GCTCTTC	1 cut(s)	2523
BspT104I	TTCGAA	2 cut(s)	1175, 2834
BspT107I	GGYRCC	1 cut(s)	293
BspTNI	GGTCTC	1 cut(s)	1874
BsrDI	GCAATG	2 cut(s)	706, 1905
BsrFI	RCCGGY	2 cut(s)	415, 1350
BsrI	ACTGG	1 cut(s)	1405
BssAI	RCCGGY	2 cut(s)	415, 1350
BssECI	CCNNGG	3 cut(s)	379, 1921, 2242
BssNAI	GTATAC	1 cut(s)	900
BssNI	GRCGYC	1 cut(s)	1307
BssSI	CACGAG	1 cut(s)	213
BssT1I	CCWWGG	3 cut(s)	379, 1921, 2242
Bst1107I	GTATAC	1 cut(s)	900
Bst2BI	CACGAG	1 cut(s)	213
Bst2UI	CCWGG	2 cut(s)	248, 1876
Bst4CI	ACNGT	7 cut(s)	127, 756, 1244, 1664, 1853, 2427, 2783
Bst6I	CTCTTC	3 cut(s)	932, 1068, 2523
BstACI	GRCGYC	1 cut(s)	1307
BstBAI	YACGTR	2 cut(s)	897, 2475
BstBI	TTCGAA	2 cut(s)	1175, 2834
BstC8I	GCNNGC	7 cut(s)	375, 417, 1982, 2565, 2659, 2969, 3043
BstDEI	CTNAG	6 cut(s)	575, 1061, 2028, 2208, 2882, 2972
BstDSI	CCRYGG	2 cut(s)	379, 2242
BstEII	GGTNACC	2 cut(s)	254, 1828
BstF5I	GGATG	9 cut(s)	121, 516, 633, 663, 736, 781, 1948, 2755, 2987
BstFNI	CGCG	1 cut(s)	1824
BstMAI	GTCTC	3 cut(s)	1874, 2031, 2739
BstMWI	GCNNNNNNNGC	5 cut(s)	370, 2508, 2607, 2616, 2945
BstNI	CCWGG	2 cut(s)	248, 1876
BstNSI	RCATGY	4 cut(s)	377, 2456, 2898, 2971
BstPI	GGTNACC	2 cut(s)	254, 1828
BstSCI	CCNGG	4 cut(s)	246, 407, 1414, 1874
BstSFI	CTRYAG	2 cut(s)	82, 2561
BstSLI	GKGCMC	1 cut(s)	298
BstSNI	TACGTA	2 cut(s)	897, 2475
BstUI	CGCG	1 cut(s)	1824
BstV1I	GCAGC	4 cut(s)	68, 520, 2344, 2960
BstV2I	GAAGAC	1 cut(s)	3033
BstX2I	RGATCY	4 cut(s)	1134, 1360, 1808, 1905
BstXI	CCANNNNNNTGG	1 cut(s)	251
BstYI	RGATCY	4 cut(s)	1134, 1360, 1808, 1905
BstZ17I	GTATAC	1 cut(s)	900
Bsu15I	ATCGAT	1 cut(s)	866
Bsu36I	CCTNAGG	1 cut(s)	1061
BsuRI	GGCC	7 cut(s)	1058, 1344, 1554, 2318, 2352, 2567, 2705
BsuTUI	ATCGAT	1 cut(s)	866
BtgI	CCRYGG	2 cut(s)	379, 2242
BtsCI	GGATG	9 cut(s)	121, 516, 633, 663, 736, 781, 1948, 2755, 2987
BtsI	GCAGTG	2 cut(s)	76, 1765
BtsIMutI	CAGTG	6 cut(s)	76, 132, 1398, 1765, 1858, 2788
BveI	ACCTGC	1 cut(s)	2291
Cac8I	GCNNGC	7 cut(s)	375, 417, 1982, 2565, 2659, 2969, 3043
Cfr10I	RCCGGY	2 cut(s)	415, 1350
Cfr13I	GGNCC	3 cut(s)	73, 405, 1057
ClaI	ATCGAT	1 cut(s)	866
CseI	GACGC	2 cut(s)	1315, 1830
CsiI	ACCWGGT	1 cut(s)	246
Csp6I	GTAC	7 cut(s)	141, 795, 911, 1009, 1240, 1660, 2476
CviQI	GTAC	7 cut(s)	141, 795, 911, 1009, 1240, 1660, 2476
DdeI	CTNAG	6 cut(s)	575, 1061, 2028, 2208, 2882, 2972
Eam1104I	CTCTTC	3 cut(s)	932, 1068, 2523
EarI	CTCTTC	3 cut(s)	932, 1068, 2523
Ecl136II	GAGCTC	2 cut(s)	183, 1918
Eco105I	TACGTA	2 cut(s)	897, 2475
Eco130I	CCWWGG	3 cut(s)	379, 1921, 2242
Eco147I	AGGCCT	3 cut(s)	2318, 2352, 2705
Eco24I	GRGCYC	2 cut(s)	185, 1920
Eco31I	GGTCTC	1 cut(s)	1874
Eco32I	GATATC	1 cut(s)	1840
Eco47I	GGWCC	2 cut(s)	73, 405
Eco53kI	GAGCTC	2 cut(s)	183, 1918
Eco57I	CTGAAG	1 cut(s)	170
Eco81I	CCTNAGG	1 cut(s)	1061
Eco88I	CYCGRG	2 cut(s)	1086, 2960
Eco91I	GGTNACC	2 cut(s)	254, 1828
EcoICRI	GAGCTC	2 cut(s)	183, 1918
EcoO109I	RGGNCCY	2 cut(s)	405, 1057
EcoO65I	GGTNACC	2 cut(s)	254, 1828
EcoRII	CCWGG	2 cut(s)	246, 1874
EcoRV	GATATC	1 cut(s)	1840
EcoT14I	CCWWGG	3 cut(s)	379, 1921, 2242
EcoT22I	ATGCAT	3 cut(s)	1276, 1633, 2380
EcoT38I	GRGCYC	2 cut(s)	185, 1920
ErhI	CCWWGG	3 cut(s)	379, 1921, 2242
FaqI	GGGAC	2 cut(s)	1833, 2108
FauI	CCCGC	1 cut(s)	1817
FbaI	TGATCA	3 cut(s)	495, 705, 1395
FblI	GTMKAC	1 cut(s)	899
Fnu4HI	GCNGC	4 cut(s)	82, 534, 2358, 2949
FokI	GGATG	9 cut(s)	128, 523, 640, 670, 743, 768, 1955, 2762, 2994
FriOI	GRGCYC	2 cut(s)	185, 1920
Fsp4HI	GCNGC	4 cut(s)	82, 534, 2358, 2949
FspBI	CTAG	1 cut(s)	1712
GluI	GCNGC	4 cut(s)	82, 534, 2358, 2949
GsaI	CCCAGC	1 cut(s)	1134
GsuI	CTGGAG	2 cut(s)	1377, 1858
HaeIII	GGCC	7 cut(s)	1058, 1344, 1554, 2318, 2352, 2567, 2705
HapII	CCGG	5 cut(s)	409, 416, 1351, 1416, 1940
HgaI	GACGC	2 cut(s)	1315, 1830
Hin1I	GRCGYC	1 cut(s)	1307
HindIII	AAGCTT	1 cut(s)	362
HpaII	CCGG	5 cut(s)	409, 416, 1351, 1416, 1940
HphI	GGTGA	5 cut(s)	266, 820, 1840, 2315, 2732
Hpy166II	GTNNAC	7 cut(s)	76, 900, 1261, 1654, 2478, 2911, 2997
Hpy8I	GTNNAC	7 cut(s)	76, 900, 1261, 1654, 2478, 2911, 2997
HpyAV	CCTTC	9 cut(s)	610, 766, 808, 871, 1153, 1381, 1486, 1754, 2711
HpyCH4III	ACNGT	7 cut(s)	127, 756, 1244, 1664, 1853, 2427, 2783
HpyCH4IV	ACGT	3 cut(s)	896, 1011, 2474
HpyF10VI	GCNNNNNNNGC	5 cut(s)	370, 2508, 2607, 2616, 2945
HpyF3I	CTNAG	6 cut(s)	575, 1061, 2028, 2208, 2882, 2972
HpySE526I	ACGT	3 cut(s)	896, 1011, 2474
Hsp92I	GRCGYC	1 cut(s)	1307
Kpn2I	TCCGGA	1 cut(s)	1939
KroI	GCCGGC	1 cut(s)	415
KroNI	GCCGGC	1 cut(s)	417
Ksp22I	TGATCA	3 cut(s)	495, 705, 1395
LguI	GCTCTTC	1 cut(s)	2523
LmnI	GCTCC	4 cut(s)	315, 1249, 1732, 1923
Lsp1109I	GCAGC	4 cut(s)	68, 520, 2344, 2960
LweI	GCATC	5 cut(s)	1465, 1698, 2113, 2223, 2785
MabI	ACCWGGT	1 cut(s)	246
MaeI	CTAG	1 cut(s)	1712
MaeII	ACGT	3 cut(s)	896, 1011, 2474
MfeI	CAATTG	1 cut(s)	299
MflI	RGATCY	4 cut(s)	1134, 1360, 1808, 1905
MhlI	GDGCHC	5 cut(s)	185, 298, 312, 1920, 2883
MlyI	GAGTC	3 cut(s)	179, 2658, 3002
MmeI	TCCRAC	2 cut(s)	369, 2032
Mph1103I	ATGCAT	3 cut(s)	1276, 1633, 2380
MroI	TCCGGA	1 cut(s)	1939
MroNI	GCCGGC	1 cut(s)	415
MroXI	GAANNNNTTC	1 cut(s)	2003
MslI	CAYNNNNRTG	1 cut(s)	378
MspI	CCGG	5 cut(s)	409, 416, 1351, 1416, 1940
MspR9I	CCNGG	4 cut(s)	248, 409, 1416, 1876
MunI	CAATTG	1 cut(s)	299
Mva1269I	GAATGC	4 cut(s)	322, 590, 1192, 2941
MvaI	CCWGG	2 cut(s)	248, 1876
MvnI	CGCG	1 cut(s)	1824
MwoI	GCNNNNNNNGC	5 cut(s)	370, 2508, 2607, 2616, 2945
NaeI	GCCGGC	1 cut(s)	417
NciI	CCSGG	2 cut(s)	409, 1416
NcoI	CCATGG	2 cut(s)	379, 2242
NgoMIV	GCCGGC	1 cut(s)	415
NlaIV	GGNNCC	4 cut(s)	74, 295, 407, 1938
NmuCI	GTSAC	9 cut(s)	254, 1081, 1108, 1318, 1828, 2053, 2158, 2455, 2847
NsiI	ATGCAT	3 cut(s)	1276, 1633, 2380
NspI	RCATGY	4 cut(s)	377, 2456, 2898, 2971
NspV	TTCGAA	2 cut(s)	1175, 2834
PacI	TTAATTAA	1 cut(s)	3018
PaeI	GCATGC	2 cut(s)	377, 2971
PaeR7I	CTCGAG	2 cut(s)	1086, 2960
PaqCI	CACCTGC	1 cut(s)	2291
PceI	AGGCCT	3 cut(s)	2318, 2352, 2705
PciI	ACATGT	1 cut(s)	2894
PciSI	GCTCTTC	1 cut(s)	2523
PcsI	WCGNNNNNNNCGW	1 cut(s)	220
PctI	GAATGC	4 cut(s)	322, 590, 1192, 2941
PdiI	GCCGGC	1 cut(s)	417
PdmI	GAANNNNTTC	1 cut(s)	2003
PfoI	TCCNGGA	1 cut(s)	1874
PkrI	GCNGC	4 cut(s)	83, 535, 2359, 2950
PleI	GAGTC	3 cut(s)	178, 2658, 3001
PpsI	GAGTC	3 cut(s)	178, 2658, 3001
Ppu21I	YACGTR	2 cut(s)	897, 2475
PpuMI	RGGWCCY	1 cut(s)	405
PscI	ACATGT	1 cut(s)	2894
PshBI	ATTAAT	2 cut(s)	2073, 3018
PsiI	TTATAA	1 cut(s)	90
Psp124BI	GAGCTC	2 cut(s)	185, 1920
Psp5II	RGGWCCY	1 cut(s)	405
Psp6I	CCWGG	2 cut(s)	246, 1874
PspEI	GGTNACC	2 cut(s)	254, 1828
PspFI	CCCAGC	1 cut(s)	1130
PspGI	CCWGG	2 cut(s)	246, 1874
PspN4I	GGNNCC	4 cut(s)	74, 295, 407, 1938
PspPI	GGNCC	3 cut(s)	73, 405, 1057
PspPPI	RGGWCCY	1 cut(s)	405
PspXI	VCTCGAGB	1 cut(s)	1086
PstI	CTGCAG	2 cut(s)	86, 2565
PsuI	RGATCY	4 cut(s)	1134, 1360, 1808, 1905
RsaI	GTAC	7 cut(s)	142, 796, 912, 1010, 1241, 1661, 2477
RsaNI	GTAC	7 cut(s)	141, 795, 911, 1009, 1240, 1660, 2476
RseI	CAYNNNNRTG	1 cut(s)	378
SacI	GAGCTC	2 cut(s)	185, 1920
SapI	GCTCTTC	1 cut(s)	2523
SatI	GCNGC	4 cut(s)	82, 534, 2358, 2949
Sau96I	GGNCC	3 cut(s)	73, 405, 1057
ScaI	AGTACT	1 cut(s)	912
SchI	GAGTC	3 cut(s)	179, 2658, 3002
ScrFI	CCNGG	4 cut(s)	248, 409, 1416, 1876
SduI	GDGCHC	5 cut(s)	185, 298, 312, 1920, 2883
SexAI	ACCWGGT	1 cut(s)	246
SfaNI	GCATC	5 cut(s)	1465, 1698, 2113, 2223, 2785
SfcI	CTRYAG	2 cut(s)	82, 2561
Sfr274I	CTCGAG	2 cut(s)	1086, 2960
SfuI	TTCGAA	2 cut(s)	1175, 2834
SinI	GGWCC	2 cut(s)	73, 405
SlaI	CTCGAG	2 cut(s)	1086, 2960
SmiMI	CAYNNNNRTG	1 cut(s)	378
SmlI	CTYRAG	2 cut(s)	1086, 2960
SmoI	CTYRAG	2 cut(s)	1086, 2960
SnaBI	TACGTA	2 cut(s)	897, 2475
SphI	GCATGC	2 cut(s)	377, 2971
SseBI	AGGCCT	3 cut(s)	2318, 2352, 2705
SsiI	CCGC	4 cut(s)	1000, 1104, 1824, 2661
SspI	AATATT	1 cut(s)	2572
SspMI	CTAG	1 cut(s)	1712
SstI	GAGCTC	2 cut(s)	185, 1920
StuI	AGGCCT	3 cut(s)	2318, 2352, 2705
StyD4I	CCNGG	4 cut(s)	246, 407, 1414, 1874
StyI	CCWWGG	3 cut(s)	379, 1921, 2242
TaaI	ACNGT	7 cut(s)	127, 756, 1244, 1664, 1853, 2427, 2783
TaiI	ACGT	3 cut(s)	899, 1014, 2477
TaqI	TCGA	8 cut(s)	223, 332, 866, 1087, 1175, 2700, 2834, 2961
TatI	WGTACW	1 cut(s)	910
TscAI	CASTG	6 cut(s)	83, 132, 1405, 1772, 1858, 2788
TseFI	GTSAC	9 cut(s)	254, 1081, 1108, 1318, 1828, 2053, 2158, 2455, 2847
TseI	GCWGC	4 cut(s)	81, 533, 2357, 2948
Tsp45I	GTSAC	9 cut(s)	254, 1081, 1108, 1318, 1828, 2053, 2158, 2455, 2847
TspGWI	ACGGA	4 cut(s)	163, 217, 2196, 2928
TspRI	CASTG	6 cut(s)	83, 132, 1405, 1772, 1858, 2788
VpaK11BI	GGWCC	2 cut(s)	73, 405
VspI	ATTAAT	2 cut(s)	2073, 3018
XapI	RAATTY	6 cut(s)	316, 787, 1466, 1571, 1930, 2828
XceI	RCATGY	4 cut(s)	377, 2456, 2898, 2971
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1591
XhoI	CTCGAG	2 cut(s)	1086, 2960
XmiI	GTMKAC	1 cut(s)	899
XmnI	GAANNNNTTC	1 cut(s)	2003
XspI	CTAG	1 cut(s)	1712
ZrmI	AGTACT	1 cut(s)	912
Zsp2I	ATGCAT	3 cut(s)	1276, 1633, 2380

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.