MD07G1002800.v1.1

Belongs to the precorrin methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002225
Physical Location & Seq
Forward (+)
295585 .. 298516
2932 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1002800.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGGCTCTTGTGAGTCGGATTCAGTCTCTCTCTGCCTTCTCTTCTTCCTCCACCCATTTCAGAAAACCCCACTCCTCCTCCTCCTCCTTCAATCCCATTTCTTCATTACGCTTCAACTTCACTTCCTCCAATTCCTCCTCCTCCTCCTCCTCCTCACCTTTTACAGAGAAGGCTTCTATCGAGAGATACCAGCGAGACCACTGGCTCTACAAAAACCAACATTCAGAATCCGACCAACCAGTACCCACTACCTGCTGCTCTCTCCCGCCCGATTTTGATTCCTTGAGGGAAAACGACATCGCTTTGCAGCTACCCGAGCTCAGGAACCTGCTTCAGGTGCTCAGAGAAAAGAGGCAGACTGAAGGTGATCGAGGTGGCTGCGGGCCCGGGAATGTCTTCTTGGTGGGGACGGGGCCTGGGGACCCCGAGCTCCTGACAATGAAGGCTTACAGAGTTATTCAGACTGCTGATTTGTTGCTCTATGATAGGTTGGTGTCCAATGATGTCTTAGATTTGGTTGGCTCTGGTGCTAGACTCCTCTATGTGGGCAAGACTGCTGGGTACCATAGCAGAACCCAGGAGGAGATACATGAATTGTTACTGAGTTTTGCCGAAGCTGGAGCTAATGTTGTGAGGCTAAAAGGAGGGGATCCGCTGGTGTTTGGAAGGGGTGGAGAGGAGATGGATTTTCTGCGGCAACAAGGAATTGAAGTTAACGTTATTCCAGGTATTACTGCTGCTTCAGGGATAGCAGCAAATCTAGGGATTCCGTTAACTCATCGAGGTGTCGCAAATAGTGTCAGATTTCTCACCGGCCACTCCAGGAAGGGAGGAACAGATCCTCTGTTTGTGGCAGAGAATGCAGCTGACCCTGATTCGACCTTGGTGGTTTACATGGGTTTGTCGACTCTCCCTTCTCTGGCTCAAAAGTTGGTGCATCATGGTCTATCGCCAAATACACCAGCTGTTGCAGTTGAGCGAGGGACCACACCTCAACAACGCATGGTTTTTGCAGAACTGAAGGATGTTGCAGATGAAATTGTATCAGCAGAGTTAGTATCACCAACGTTGATCATCATTGGGAAAGTAGTGGCACTTTCACCATTGTGGCCATATGCTTCAAAGGAAGTATCCTGTCTTGTAGAAGCACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120
Pfam Domains
Protein Families
TP_methylase

Protein Analysis

385

Amino Acids

41.43

Weight (kDa)

5.95

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 132 - 343 2.3e-51 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 260, 336
Acc65I GGTACC 1 cut(s) 561
AccB1I GGYRCC 1 cut(s) 561
AccI GTMKAC 1 cut(s) 905
AciI CCGC 4 cut(s) 266, 381, 653, 694
AclI AACGTT 2 cut(s) 717, 1067
AclWI GGATC 3 cut(s) 644, 657, 833
AcoI YGGCCR 2 cut(s) 814, 1109
AcuI CTGAAG 4 cut(s) 317, 381, 726, 1040
AfaI GTAC 2 cut(s) 243, 563
AfiI CCNNNNNNNGG 4 cut(s) 321, 334, 544, 919
AgsI TTSAA 4 cut(s) 91, 115, 710, 1122
AjnI CCWGG 4 cut(s) 415, 576, 724, 821
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AleI CACNNNNGTG 1 cut(s) 1105
AluBI AGCT 7 cut(s) 310, 319, 430, 617, 623, 866, 965
AluI AGCT 7 cut(s) 310, 319, 430, 617, 623, 866, 965
Alw21I GWGCWC 3 cut(s) 321, 342, 432
Alw26I GTCTC 2 cut(s) 30, 189
AlwI GGATC 3 cut(s) 644, 657, 833
Ama87I CYCGRG 3 cut(s) 314, 386, 425
AoxI GGCC 4 cut(s) 383, 413, 814, 1109
ApaI GGGCCC 1 cut(s) 387
ApeKI GCWGC 6 cut(s) 255, 307, 378, 737, 752, 863
ArsI GACNNNNNNTTYG 2 cut(s) 454, 486
Asp700I GAANNNNTTC 1 cut(s) 395
Asp718I GGTACC 1 cut(s) 561
AspS9I GGNCC 5 cut(s) 383, 384, 413, 421, 984
AsuC2I CCSGG 2 cut(s) 387, 388
AsuHPI GGTGA 5 cut(s) 147, 377, 802, 1053, 1092
AvaI CYCGRG 3 cut(s) 314, 386, 425
AvaII GGWCC 2 cut(s) 421, 984
BaeGI GKGCMC 1 cut(s) 387
BalI TGGCCA 1 cut(s) 1111
BamHI GGATCC 1 cut(s) 649
BanI GGYRCC 1 cut(s) 561
BanII GRGCYC 3 cut(s) 321, 387, 432
BbsI GAAGAC 1 cut(s) 388
Bbv12I GWGCWC 3 cut(s) 321, 342, 432
BbvI GCAGC 6 cut(s) 242, 319, 365, 724, 764, 875
BccI CCATC 1 cut(s) 676
BcgI CGANNNNNNTGC 2 cut(s) 360, 394
BciT130I CCWGG 4 cut(s) 417, 578, 726, 823
BciVI GTATCC 1 cut(s) 1141
BclI TGATCA 1 cut(s) 1071
BcnI CCSGG 2 cut(s) 387, 388
BcoDI GTCTC 2 cut(s) 30, 189
BfaI CTAG 2 cut(s) 531, 761
BfmI CTRYAG 1 cut(s) 1151
BfuAI ACCTGC 2 cut(s) 260, 336
BfuI GTATCC 1 cut(s) 1141
BisI GCNGC 7 cut(s) 256, 308, 379, 695, 738, 753, 864
BlsI GCNGC 7 cut(s) 257, 309, 380, 696, 739, 754, 865
Bme1390I CCNGG 6 cut(s) 387, 388, 417, 578, 726, 823
Bme18I GGWCC 2 cut(s) 421, 984
BmeT110I CYCGRG 3 cut(s) 314, 386, 425
BmgT120I GGNCC 5 cut(s) 383, 384, 413, 421, 984
BmiI GGNNCC 8 cut(s) 326, 385, 414, 422, 423, 563, 651, 985
BmrFI CCNGG 6 cut(s) 387, 388, 417, 578, 726, 823
BmsI GCATC 1 cut(s) 946
BpiI GAAGAC 1 cut(s) 388
BpmI CTGGAG 2 cut(s) 639, 805
Bpu10I CCTNAGC 1 cut(s) 320
BpuEI CTTGAG 1 cut(s) 304
BpuMI CCSGG 2 cut(s) 387, 388
BsaI GGTCTC 1 cut(s) 189
BsaJI CCNNGG 4 cut(s) 386, 416, 576, 882
BsaXI ACNNNNNCTCC 4 cut(s) 62, 92, 612, 642
Bsc4I CCNNNNNNNGG 4 cut(s) 321, 334, 544, 919
Bse118I RCCGGY 1 cut(s) 812
Bse1I ACTGG 2 cut(s) 206, 239
BseBI CCWGG 4 cut(s) 417, 578, 726, 823
BseDI CCNNGG 4 cut(s) 386, 416, 576, 882
BseGI GGATG 1 cut(s) 1030
BseLI CCNNNNNNNGG 4 cut(s) 321, 334, 544, 919
BseMII CTCAG 3 cut(s) 334, 355, 593
BseNI ACTGG 2 cut(s) 206, 239
BseSI GKGCMC 1 cut(s) 387
BseXI GCAGC 6 cut(s) 242, 319, 365, 724, 764, 875
BseYI CCCAGC 1 cut(s) 557
BshFI GGCC 4 cut(s) 385, 415, 816, 1111
BshNI GGYRCC 1 cut(s) 561
BsiHKAI GWGCWC 3 cut(s) 321, 342, 432
BsiHKCI CYCGRG 3 cut(s) 314, 386, 425
BsiSI CCGG 2 cut(s) 387, 813
BslFI GGGAC 3 cut(s) 421, 434, 997
BslI CCNNNNNNNGG 4 cut(s) 321, 334, 544, 919
BsmAI GTCTC 2 cut(s) 30, 189
BsmFI GGGAC 3 cut(s) 421, 434, 997
BsmI GAATGC 1 cut(s) 865
BsnI GGCC 4 cut(s) 385, 415, 816, 1111
Bso31I GGTCTC 1 cut(s) 189
BsoBI CYCGRG 3 cut(s) 314, 386, 425
Bsp120I GGGCCC 1 cut(s) 383
Bsp1286I GDGCHC 4 cut(s) 321, 342, 387, 432
Bsp143I GATC 4 cut(s) 367, 649, 838, 1071
BspACI CCGC 4 cut(s) 266, 381, 653, 694
BspANI GGCC 4 cut(s) 385, 415, 816, 1111
BspCNI CTCAG 3 cut(s) 333, 354, 594
BspLI GGNNCC 8 cut(s) 326, 385, 414, 422, 423, 563, 651, 985
BspMI ACCTGC 2 cut(s) 260, 336
BspPI GGATC 3 cut(s) 644, 657, 833
BspT107I GGYRCC 1 cut(s) 561
BspTNI GGTCTC 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 812
BsrI ACTGG 2 cut(s) 206, 239
BssAI RCCGGY 1 cut(s) 812
BssECI CCNNGG 4 cut(s) 386, 416, 576, 882
BssMI GATC 4 cut(s) 367, 649, 838, 1071
BssT1I CCWWGG 1 cut(s) 882
Bst2UI CCWGG 4 cut(s) 417, 578, 726, 823
Bst6I CTCTTC 1 cut(s) 46
BstAPI GCANNNNNTGC 1 cut(s) 860
BstC8I GCNNGC 1 cut(s) 383
BstDEI CTNAG 4 cut(s) 320, 341, 508, 602
BstENI CCTNNNNNAGG 1 cut(s) 332
BstF5I GGATG 1 cut(s) 1030
BstKTI GATC 4 cut(s) 370, 652, 841, 1074
BstMAI GTCTC 2 cut(s) 30, 189
BstMBI GATC 4 cut(s) 367, 649, 838, 1071
BstMWI GCNNNNNNNGC 3 cut(s) 316, 337, 860
BstNI CCWGG 4 cut(s) 417, 578, 726, 823
BstSCI CCNGG 6 cut(s) 385, 386, 415, 576, 724, 821
BstSFI CTRYAG 1 cut(s) 1151
BstSLI GKGCMC 1 cut(s) 387
BstV1I GCAGC 6 cut(s) 242, 319, 365, 724, 764, 875
BstV2I GAAGAC 1 cut(s) 388
BstX2I RGATCY 2 cut(s) 649, 838
BstYI RGATCY 2 cut(s) 649, 838
BsuI GTATCC 1 cut(s) 1141
BsuRI GGCC 4 cut(s) 385, 415, 816, 1111
BtgZI GCGATG 1 cut(s) 283
BtsCI GGATG 1 cut(s) 1030
BtsIMutI CAGTG 1 cut(s) 199
BveI ACCTGC 2 cut(s) 260, 336
Cac8I GCNNGC 1 cut(s) 383
Cfr10I RCCGGY 1 cut(s) 812
Cfr13I GGNCC 5 cut(s) 383, 384, 413, 421, 984
Cfr9I CCCGGG 1 cut(s) 386
Csp6I GTAC 2 cut(s) 242, 562
CviAII CATG 4 cut(s) 590, 895, 941, 1003
CviQI GTAC 2 cut(s) 242, 562
DdeI CTNAG 4 cut(s) 320, 341, 508, 602
DpnI GATC 4 cut(s) 369, 651, 840, 1073
DpnII GATC 4 cut(s) 367, 649, 838, 1071
EaeI YGGCCR 2 cut(s) 814, 1109
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
Ecl136II GAGCTC 2 cut(s) 319, 430
Eco130I CCWWGG 1 cut(s) 882
Eco24I GRGCYC 3 cut(s) 321, 387, 432
Eco31I GGTCTC 1 cut(s) 189
Eco47I GGWCC 2 cut(s) 421, 984
Eco53kI GAGCTC 2 cut(s) 319, 430
Eco57I CTGAAG 4 cut(s) 317, 381, 726, 1040
Eco88I CYCGRG 3 cut(s) 314, 386, 425
EcoICRI GAGCTC 2 cut(s) 319, 430
EcoNI CCTNNNNNAGG 1 cut(s) 332
EcoO109I RGGNCCY 2 cut(s) 413, 421
EcoRII CCWGG 4 cut(s) 415, 576, 724, 821
EcoT14I CCWWGG 1 cut(s) 882
EcoT38I GRGCYC 3 cut(s) 321, 387, 432
ErhI CCWWGG 1 cut(s) 882
FaeI CATG 4 cut(s) 593, 898, 944, 1006
FaqI GGGAC 3 cut(s) 421, 434, 997
FatI CATG 4 cut(s) 589, 894, 940, 1002
FauI CCCGC 2 cut(s) 273, 374
FauNDI CATATG 1 cut(s) 1114
FbaI TGATCA 1 cut(s) 1071
FblI GTMKAC 1 cut(s) 905
Fnu4HI GCNGC 7 cut(s) 256, 308, 379, 695, 738, 753, 864
FokI GGATG 1 cut(s) 1037
FriOI GRGCYC 3 cut(s) 321, 387, 432
Fsp4HI GCNGC 7 cut(s) 256, 308, 379, 695, 738, 753, 864
FspBI CTAG 2 cut(s) 531, 761
GluI GCNGC 7 cut(s) 256, 308, 379, 695, 738, 753, 864
GsaI CCCAGC 1 cut(s) 561
GsuI CTGGAG 2 cut(s) 639, 805
HaeIII GGCC 4 cut(s) 385, 415, 816, 1111
HapII CCGG 2 cut(s) 387, 813
Hin1II CATG 4 cut(s) 593, 898, 944, 1006
HincII GTYRAC 3 cut(s) 715, 774, 906
HindII GTYRAC 3 cut(s) 715, 774, 906
HinfI GANTC 8 cut(s) 13, 19, 227, 278, 534, 766, 875, 907
HpaI GTTAAC 2 cut(s) 715, 774
HpaII CCGG 2 cut(s) 387, 813
HphI GGTGA 5 cut(s) 147, 377, 802, 1053, 1092
Hpy166II GTNNAC 4 cut(s) 715, 774, 892, 906
Hpy188I TCNGA 7 cut(s) 18, 62, 226, 232, 344, 462, 803
Hpy188III TCNNGA 3 cut(s) 181, 322, 433
Hpy8I GTNNAC 4 cut(s) 715, 774, 892, 906
HpyAV CCTTC 9 cut(s) 46, 97, 163, 356, 436, 660, 820, 924, 1015
HpyCH4IV ACGT 2 cut(s) 717, 1067
HpyCH4V TGCA 6 cut(s) 307, 863, 937, 971, 1013, 1031
HpyF10VI GCNNNNNNNGC 3 cut(s) 316, 337, 860
HpyF3I CTNAG 4 cut(s) 320, 341, 508, 602
HpySE526I ACGT 2 cut(s) 717, 1067
Hsp92II CATG 4 cut(s) 593, 898, 944, 1006
KflI GGGWCCC 1 cut(s) 421
KpnI GGTACC 1 cut(s) 565
Ksp22I TGATCA 1 cut(s) 1071
KspAI GTTAAC 2 cut(s) 715, 774
Kzo9I GATC 4 cut(s) 367, 649, 838, 1071
LmnI GCTCC 2 cut(s) 435, 620
Lsp1109I GCAGC 6 cut(s) 242, 319, 365, 724, 764, 875
LweI GCATC 1 cut(s) 946
MaeI CTAG 2 cut(s) 531, 761
MaeII ACGT 2 cut(s) 717, 1067
MaeIII GTNAC 1 cut(s) 597
MalI GATC 4 cut(s) 369, 651, 840, 1073
MboI GATC 4 cut(s) 367, 649, 838, 1071
MboII GAAGA 4 cut(s) 33, 36, 93, 388
MflI RGATCY 2 cut(s) 649, 838
MhlI GDGCHC 4 cut(s) 321, 342, 387, 432
MlsI TGGCCA 1 cut(s) 1111
MluCI AATT 4 cut(s) 130, 593, 705, 1038
MluNI TGGCCA 1 cut(s) 1111
MlyI GAGTC 3 cut(s) 22, 528, 901
MmeI TCCRAC 1 cut(s) 255
Mox20I TGGCCA 1 cut(s) 1111
MroXI GAANNNNTTC 1 cut(s) 395
MscI TGGCCA 1 cut(s) 1111
MseI TTAA 2 cut(s) 714, 773
MslI CAYNNNNRTG 2 cut(s) 783, 1105
Msp20I TGGCCA 1 cut(s) 1111
MspA1I CMGCKG 3 cut(s) 655, 866, 965
MspI CCGG 2 cut(s) 387, 813
MspR9I CCNGG 6 cut(s) 387, 388, 417, 578, 726, 823
Mva1269I GAATGC 1 cut(s) 865
MvaI CCWGG 4 cut(s) 417, 578, 726, 823
MwoI GCNNNNNNNGC 3 cut(s) 316, 337, 860
NciI CCSGG 2 cut(s) 387, 388
NdeI CATATG 1 cut(s) 1114
NdeII GATC 4 cut(s) 367, 649, 838, 1071
NlaIII CATG 4 cut(s) 593, 898, 944, 1006
NlaIV GGNNCC 8 cut(s) 326, 385, 414, 422, 423, 563, 651, 985
OliI CACNNNNGTG 1 cut(s) 1105
PctI GAATGC 1 cut(s) 865
PdmI GAANNNNTTC 1 cut(s) 395
PfeI GAWTC 5 cut(s) 19, 227, 278, 766, 875
PfoI TCCNGGA 1 cut(s) 821
PkrI GCNGC 7 cut(s) 257, 309, 380, 696, 739, 754, 865
PleI GAGTC 3 cut(s) 21, 528, 901
PpsI GAGTC 3 cut(s) 21, 528, 901
PpuMI RGGWCCY 1 cut(s) 421
Psp124BI GAGCTC 2 cut(s) 321, 432
Psp1406I AACGTT 2 cut(s) 717, 1067
Psp5II RGGWCCY 1 cut(s) 421
Psp6I CCWGG 4 cut(s) 415, 576, 724, 821
PspFI CCCAGC 1 cut(s) 557
PspGI CCWGG 4 cut(s) 415, 576, 724, 821
PspN4I GGNNCC 8 cut(s) 326, 385, 414, 422, 423, 563, 651, 985
PspOMI GGGCCC 1 cut(s) 383
PspPI GGNCC 5 cut(s) 383, 384, 413, 421, 984
PspPPI RGGWCCY 1 cut(s) 421
PsuI RGATCY 2 cut(s) 649, 838
PvuII CAGCTG 2 cut(s) 866, 965
RsaI GTAC 2 cut(s) 243, 563
RsaNI GTAC 2 cut(s) 242, 562
RseI CAYNNNNRTG 2 cut(s) 783, 1105
SacI GAGCTC 2 cut(s) 321, 432
SalI GTCGAC 1 cut(s) 904
SaqAI TTAA 2 cut(s) 714, 773
SatI GCNGC 7 cut(s) 256, 308, 379, 695, 738, 753, 864
Sau3AI GATC 4 cut(s) 367, 649, 838, 1071
Sau96I GGNCC 5 cut(s) 383, 384, 413, 421, 984
SchI GAGTC 3 cut(s) 22, 528, 901
ScrFI CCNGG 6 cut(s) 387, 388, 417, 578, 726, 823
SduI GDGCHC 4 cut(s) 321, 342, 387, 432
SfaNI GCATC 1 cut(s) 946
SfcI CTRYAG 1 cut(s) 1151
SinI GGWCC 2 cut(s) 421, 984
SmaI CCCGGG 1 cut(s) 388
SmiMI CAYNNNNRTG 2 cut(s) 783, 1105
SmlI CTYRAG 1 cut(s) 283
SmoI CTYRAG 1 cut(s) 283
Sse9I AATT 4 cut(s) 130, 593, 705, 1038
SsiI CCGC 4 cut(s) 266, 381, 653, 694
SspMI CTAG 2 cut(s) 531, 761
SstI GAGCTC 2 cut(s) 321, 432
StyD4I CCNGG 6 cut(s) 385, 386, 415, 576, 724, 821
StyI CCWWGG 1 cut(s) 882
TaiI ACGT 2 cut(s) 720, 1070
TaqI TCGA 5 cut(s) 180, 370, 781, 878, 905
TasI AATT 4 cut(s) 130, 593, 705, 1038
TauI GCSGC 1 cut(s) 697
TfiI GAWTC 5 cut(s) 19, 227, 278, 766, 875
Tru1I TTAA 2 cut(s) 714, 773
Tru9I TTAA 2 cut(s) 714, 773
TscAI CASTG 1 cut(s) 206
TseI GCWGC 6 cut(s) 255, 307, 378, 737, 752, 863
TspDTI ATGAA 4 cut(s) 93, 455, 606, 1050
TspGWI ACGGA 1 cut(s) 759
TspMI CCCGGG 1 cut(s) 386
TspRI CASTG 1 cut(s) 206
VpaK11BI GGWCC 2 cut(s) 421, 984
XagI CCTNNNNNAGG 1 cut(s) 332
XmaI CCCGGG 1 cut(s) 386
XmiI GTMKAC 1 cut(s) 905
XmnI GAANNNNTTC 1 cut(s) 395
XspI CTAG 2 cut(s) 531, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.