Rh6BG225200

Belongs to the precorrin methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002225
Physical Location & Seq
Reverse (-)
42591048 .. 42595983
4936 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG225200.1

Sequence Viewer

Length: 1113 bp
ATGGCTCTTGTGAGTAGGTTTCAATCTCTGTCTTCCTCTTTGTCTTCCACCCATTTCAGAAAACCCAACTGCTTAAACCCTGAACCCATTTGCTCTCTACACTTCAACTCCCCTTCTTACACTTCTCCTTTTACAGAGAAGCATTCAATTGAGAGGTACCAGAGAGACCGGTGGCTATACAATAACCAATTGGAGCAACCCACTGTAATCTCTCTCCCTCCTGATTCTGATTTCACAAGGCAGAATGACATTGCTTTGCAGCTACCAGAACTGAGAAAGCTGCTTCAGGTGCTGCGAGAGAAGAGAGAGAGGGAAGGAGGGTGTGGTGGAGGCAAGTGTGGGCCTGGCAATGTCTTCTTGGTGGGCACTGGCCCTGGGGACCCTGAACTCCTGACATTGAAGGCTGTCAGAGTTATTCAGAGTGCTGACTTATTGTTGTATGATAGGTTGGTGTCCAATGATGTGTTGGATTTTGTTGCCTCTGATGCTAGACTTGTCTATGTGGGCAAGACTGCTGGGTACCATAGCAGAACCCAGGAGGAGATACATGAATTGCTACTGAGTTTTGCTGAAGCCGGAGCTACTGTTGTGAGATTAAAAGGAGGGGATCCACTGGTGTTTGGAAGGGGTGGAGAGGAGATGGATTTTTTGCAACAGCAAGGAATTCAAGTGCAAGTTATTCCTGGTATCACTGCTGCTTCTGGGATAGCAGCAGAGCTTGGGATCCCATTAACTCATAGAGGTGTTGCAAATAGTGTAAGATTTCTGACCGGGCACTCCAGGAAGGGAGGAACAGATCCTCTTTTTGTGACAGAGAATGCAGCTGACCCTGATTCGACCTTGGTGGTATACATGGGTTTGTCGACTCTCCCTTCTCTTGCTCAAAAGTTGGTCCATCATGGTCTGCCACCAACAACACCAGCTGTTGCAATTGAGCGAGGGACCACACCTCAACAGCGCATGGTCTTTGCAGAACTGAAGGATCTTGCCAATGAAATTACATCAGCAGAGTTAGTATCACCAACGCTCATCGTCATTGGGAAAGTAGTTGCACTTTCACCATTGTGGCCACATAATTTGAATGAAGTGGTCTCTCTTGTTGGAGCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120
Pfam Domains
Protein Families
TP_methylase

Protein Analysis

370

Amino Acids

40.15

Weight (kDa)

6.08

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 118 - 330 4.4e-52 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 156, 519
AccB1I GGYRCC 2 cut(s) 156, 519
AccI GTMKAC 2 cut(s) 849, 863
AclWI GGATC 6 cut(s) 602, 615, 718, 731, 791, 990
AcoI YGGCCR 1 cut(s) 1067
AcsI RAATTY 1 cut(s) 663
AcuI CTGAAG 3 cut(s) 269, 591, 998
AfaI GTAC 2 cut(s) 158, 521
AgeI ACCGGT 1 cut(s) 168
AgsI TTSAA 6 cut(s) 23, 106, 147, 400, 668, 1081
AjnI CCWGG 5 cut(s) 343, 373, 534, 682, 779
AleI CACNNNNGTG 1 cut(s) 1063
AluBI AGCT 6 cut(s) 262, 280, 581, 718, 824, 923
AluI AGCT 6 cut(s) 262, 280, 581, 718, 824, 923
Alw26I GTCTC 2 cut(s) 159, 1096
AlwI GGATC 6 cut(s) 602, 615, 718, 731, 791, 990
AlwNI CAGNNNCTG 1 cut(s) 292
AoxI GGCC 3 cut(s) 341, 370, 1067
ApeKI GCWGC 6 cut(s) 259, 280, 292, 695, 710, 821
ApoI RAATTY 1 cut(s) 663
AsiGI ACCGGT 1 cut(s) 168
Asp718I GGTACC 2 cut(s) 156, 519
AspLEI GCGC 1 cut(s) 960
AspS9I GGNCC 5 cut(s) 341, 371, 379, 892, 942
AsuC2I CCSGG 1 cut(s) 772
AsuHPI GGTGA 2 cut(s) 1011, 1050
AvaII GGWCC 3 cut(s) 379, 892, 942
BaeGI GKGCMC 2 cut(s) 368, 777
BalI TGGCCA 1 cut(s) 1069
BamHI GGATCC 2 cut(s) 607, 723
BanI GGYRCC 2 cut(s) 156, 519
BbsI GAAGAC 3 cut(s) 24, 36, 346
BbvI GCAGC 6 cut(s) 267, 271, 279, 682, 722, 833
BccI CCATC 2 cut(s) 634, 903
BciT130I CCWGG 5 cut(s) 345, 375, 536, 684, 781
BcnI CCSGG 1 cut(s) 772
BcoDI GTCTC 2 cut(s) 159, 1096
BfaI CTAG 1 cut(s) 489
BisI GCNGC 6 cut(s) 260, 281, 293, 696, 711, 822
BlsI GCNGC 6 cut(s) 261, 282, 294, 697, 712, 823
Bme1390I CCNGG 6 cut(s) 345, 375, 536, 684, 772, 781
Bme18I GGWCC 3 cut(s) 379, 892, 942
BmgT120I GGNCC 5 cut(s) 341, 371, 379, 892, 942
BmiI GGNNCC 8 cut(s) 158, 380, 381, 521, 609, 725, 943, 1105
BmrFI CCNGG 6 cut(s) 345, 375, 536, 684, 772, 781
BmsI GCATC 1 cut(s) 475
BpiI GAAGAC 3 cut(s) 24, 36, 346
BpmI CTGGAG 1 cut(s) 763
BpuMI CCSGG 1 cut(s) 772
BsaI GGTCTC 2 cut(s) 159, 1096
BsaJI CCNNGG 4 cut(s) 373, 374, 534, 840
BsaWI WCCGGW 1 cut(s) 168
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 570, 600
Bse118I RCCGGY 1 cut(s) 168
Bse1I ACTGG 2 cut(s) 373, 618
Bse3DI GCAATG 2 cut(s) 249, 355
BseBI CCWGG 5 cut(s) 345, 375, 536, 684, 781
BseDI CCNNGG 4 cut(s) 373, 374, 534, 840
BseMI GCAATG 2 cut(s) 249, 355
BseMII CTCAG 2 cut(s) 263, 551
BseNI ACTGG 2 cut(s) 373, 618
BseRI GAGGAG 2 cut(s) 554, 650
BseSI GKGCMC 2 cut(s) 368, 777
BseXI GCAGC 6 cut(s) 267, 271, 279, 682, 722, 833
BseYI CCCAGC 1 cut(s) 515
BshFI GGCC 3 cut(s) 343, 372, 1069
BshNI GGYRCC 2 cut(s) 156, 519
BshTI ACCGGT 1 cut(s) 168
BsiSI CCGG 3 cut(s) 169, 576, 771
BslFI GGGAC 2 cut(s) 392, 955
BsmAI GTCTC 2 cut(s) 159, 1096
BsmFI GGGAC 2 cut(s) 392, 955
BsmI GAATGC 2 cut(s) 142, 823
BsnI GGCC 3 cut(s) 343, 372, 1069
Bso31I GGTCTC 2 cut(s) 159, 1096
Bsp1286I GDGCHC 2 cut(s) 368, 777
Bsp143I GATC 4 cut(s) 607, 723, 796, 982
BspANI GGCC 3 cut(s) 343, 372, 1069
BspCNI CTCAG 2 cut(s) 264, 552
BspLI GGNNCC 8 cut(s) 158, 380, 381, 521, 609, 725, 943, 1105
BspPI GGATC 6 cut(s) 602, 615, 718, 731, 791, 990
BspT107I GGYRCC 2 cut(s) 156, 519
BspTNI GGTCTC 2 cut(s) 159, 1096
BsrDI GCAATG 2 cut(s) 249, 355
BsrFI RCCGGY 1 cut(s) 168
BsrI ACTGG 2 cut(s) 373, 618
BssAI RCCGGY 1 cut(s) 168
BssECI CCNNGG 4 cut(s) 373, 374, 534, 840
BssMI GATC 4 cut(s) 607, 723, 796, 982
BssNAI GTATAC 1 cut(s) 850
BssT1I CCWWGG 1 cut(s) 840
Bst1107I GTATAC 1 cut(s) 850
Bst2UI CCWGG 5 cut(s) 345, 375, 536, 684, 781
Bst4CI ACNGT 2 cut(s) 205, 586
Bst6I CTCTTC 1 cut(s) 296
BstDEI CTNAG 2 cut(s) 272, 560
BstHHI GCGC 1 cut(s) 960
BstKTI GATC 4 cut(s) 610, 726, 799, 985
BstMAI GTCTC 2 cut(s) 159, 1096
BstMBI GATC 4 cut(s) 607, 723, 796, 982
BstMWI GCNNNNNNNGC 2 cut(s) 289, 485
BstNI CCWGG 5 cut(s) 345, 375, 536, 684, 781
BstSCI CCNGG 6 cut(s) 343, 373, 534, 682, 770, 779
BstSLI GKGCMC 2 cut(s) 368, 777
BstV1I GCAGC 6 cut(s) 267, 271, 279, 682, 722, 833
BstV2I GAAGAC 3 cut(s) 24, 36, 346
BstX2I RGATCY 4 cut(s) 607, 723, 796, 982
BstYI RGATCY 4 cut(s) 607, 723, 796, 982
BstZ17I GTATAC 1 cut(s) 850
BsuRI GGCC 3 cut(s) 343, 372, 1069
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 4 cut(s) 201, 366, 611, 690
CaiI CAGNNNCTG 1 cut(s) 292
CfoI GCGC 1 cut(s) 960
Cfr10I RCCGGY 1 cut(s) 168
Cfr13I GGNCC 5 cut(s) 341, 371, 379, 892, 942
Csp6I GTAC 2 cut(s) 157, 520
CspAI ACCGGT 1 cut(s) 168
CspCI CAANNNNNGTGG 2 cut(s) 1059, 1094
CviAII CATG 4 cut(s) 548, 853, 899, 961
CviQI GTAC 2 cut(s) 157, 520
DdeI CTNAG 2 cut(s) 272, 560
DpnI GATC 4 cut(s) 609, 725, 798, 984
DpnII GATC 4 cut(s) 607, 723, 796, 982
EaeI YGGCCR 1 cut(s) 1067
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Eco130I CCWWGG 1 cut(s) 840
Eco31I GGTCTC 2 cut(s) 159, 1096
Eco47I GGWCC 3 cut(s) 379, 892, 942
Eco57I CTGAAG 3 cut(s) 269, 591, 998
EcoO109I RGGNCCY 1 cut(s) 379
EcoRI GAATTC 1 cut(s) 663
EcoRII CCWGG 5 cut(s) 343, 373, 534, 682, 779
EcoT14I CCWWGG 1 cut(s) 840
ErhI CCWWGG 1 cut(s) 840
FaeI CATG 4 cut(s) 551, 856, 902, 964
FaqI GGGAC 2 cut(s) 392, 955
FatI CATG 4 cut(s) 547, 852, 898, 960
FblI GTMKAC 2 cut(s) 849, 863
Fnu4HI GCNGC 6 cut(s) 260, 281, 293, 696, 711, 822
Fsp4HI GCNGC 6 cut(s) 260, 281, 293, 696, 711, 822
FspBI CTAG 1 cut(s) 489
GlaI GCGC 1 cut(s) 959
GluI GCNGC 6 cut(s) 260, 281, 293, 696, 711, 822
GsaI CCCAGC 1 cut(s) 519
GsuI CTGGAG 1 cut(s) 763
HaeIII GGCC 3 cut(s) 343, 372, 1069
HapII CCGG 3 cut(s) 169, 576, 771
HhaI GCGC 1 cut(s) 960
Hin1II CATG 4 cut(s) 551, 856, 902, 964
Hin6I GCGC 1 cut(s) 958
HinP1I GCGC 1 cut(s) 958
HincII GTYRAC 1 cut(s) 864
HindII GTYRAC 1 cut(s) 864
HinfI GANTC 3 cut(s) 224, 833, 865
HpaII CCGG 3 cut(s) 169, 576, 771
HphI GGTGA 2 cut(s) 1011, 1050
Hpy166II GTNNAC 2 cut(s) 850, 864
Hpy188I TCNGA 6 cut(s) 59, 229, 410, 420, 484, 768
Hpy188III TCNNGA 2 cut(s) 221, 391
Hpy8I GTNNAC 2 cut(s) 850, 864
HpyAV CCTTC 7 cut(s) 123, 308, 394, 618, 778, 882, 973
HpyCH4III ACNGT 2 cut(s) 205, 586
HpyCH4V TGCA 8 cut(s) 259, 652, 673, 749, 821, 929, 971, 1052
HpyF10VI GCNNNNNNNGC 2 cut(s) 289, 485
HpyF3I CTNAG 2 cut(s) 272, 560
Hsp92II CATG 4 cut(s) 551, 856, 902, 964
HspAI GCGC 1 cut(s) 958
KflI GGGWCCC 1 cut(s) 379
KpnI GGTACC 2 cut(s) 160, 523
Kzo9I GATC 4 cut(s) 607, 723, 796, 982
LmnI GCTCC 3 cut(s) 193, 578, 1103
Lsp1109I GCAGC 6 cut(s) 267, 271, 279, 682, 722, 833
LweI GCATC 1 cut(s) 475
MaeI CTAG 1 cut(s) 489
MaeIII GTNAC 1 cut(s) 808
MalI GATC 4 cut(s) 609, 725, 798, 984
MboI GATC 4 cut(s) 607, 723, 796, 982
MboII GAAGA 4 cut(s) 24, 36, 313, 346
MfeI CAATTG 3 cut(s) 147, 188, 930
MflI RGATCY 4 cut(s) 607, 723, 796, 982
MhlI GDGCHC 2 cut(s) 368, 777
MlsI TGGCCA 1 cut(s) 1069
MluCI AATT 7 cut(s) 147, 188, 551, 663, 930, 996, 1075
MluNI TGGCCA 1 cut(s) 1069
MlyI GAGTC 1 cut(s) 859
MmeI TCCRAC 2 cut(s) 447, 1081
Mox20I TGGCCA 1 cut(s) 1069
MscI TGGCCA 1 cut(s) 1069
MseI TTAA 3 cut(s) 74, 596, 731
MslI CAYNNNNRTG 2 cut(s) 741, 1063
Msp20I TGGCCA 1 cut(s) 1069
MspA1I CMGCKG 2 cut(s) 824, 923
MspI CCGG 3 cut(s) 169, 576, 771
MspR9I CCNGG 6 cut(s) 345, 375, 536, 684, 772, 781
MunI CAATTG 3 cut(s) 147, 188, 930
Mva1269I GAATGC 2 cut(s) 142, 823
MvaI CCWGG 5 cut(s) 345, 375, 536, 684, 781
MwoI GCNNNNNNNGC 2 cut(s) 289, 485
NciI CCSGG 1 cut(s) 772
NdeII GATC 4 cut(s) 607, 723, 796, 982
NlaIII CATG 4 cut(s) 551, 856, 902, 964
NlaIV GGNNCC 8 cut(s) 158, 380, 381, 521, 609, 725, 943, 1105
NmuCI GTSAC 1 cut(s) 808
OliI CACNNNNGTG 1 cut(s) 1063
PasI CCCWGGG 1 cut(s) 374
PctI GAATGC 2 cut(s) 142, 823
PfeI GAWTC 2 cut(s) 224, 833
PfoI TCCNGGA 1 cut(s) 779
PinAI ACCGGT 1 cut(s) 168
PkrI GCNGC 6 cut(s) 261, 282, 294, 697, 712, 823
PleI GAGTC 1 cut(s) 859
PpsI GAGTC 1 cut(s) 859
PpuMI RGGWCCY 1 cut(s) 379
Psp5II RGGWCCY 1 cut(s) 379
Psp6I CCWGG 5 cut(s) 343, 373, 534, 682, 779
PspFI CCCAGC 1 cut(s) 515
PspGI CCWGG 5 cut(s) 343, 373, 534, 682, 779
PspN4I GGNNCC 8 cut(s) 158, 380, 381, 521, 609, 725, 943, 1105
PspPI GGNCC 5 cut(s) 341, 371, 379, 892, 942
PspPPI RGGWCCY 1 cut(s) 379
PstNI CAGNNNCTG 1 cut(s) 292
PsuI RGATCY 4 cut(s) 607, 723, 796, 982
PvuII CAGCTG 2 cut(s) 824, 923
RsaI GTAC 2 cut(s) 158, 521
RsaNI GTAC 2 cut(s) 157, 520
RseI CAYNNNNRTG 2 cut(s) 741, 1063
SalI GTCGAC 1 cut(s) 862
SaqAI TTAA 3 cut(s) 74, 596, 731
SatI GCNGC 6 cut(s) 260, 281, 293, 696, 711, 822
Sau3AI GATC 4 cut(s) 607, 723, 796, 982
Sau96I GGNCC 5 cut(s) 341, 371, 379, 892, 942
SchI GAGTC 1 cut(s) 859
ScrFI CCNGG 6 cut(s) 345, 375, 536, 684, 772, 781
SduI GDGCHC 2 cut(s) 368, 777
SfaNI GCATC 1 cut(s) 475
SinI GGWCC 3 cut(s) 379, 892, 942
SmiMI CAYNNNNRTG 2 cut(s) 741, 1063
Sse9I AATT 7 cut(s) 147, 188, 551, 663, 930, 996, 1075
SspMI CTAG 1 cut(s) 489
StyD4I CCNGG 6 cut(s) 343, 373, 534, 682, 770, 779
StyI CCWWGG 1 cut(s) 840
TaaI ACNGT 2 cut(s) 205, 586
TaqI TCGA 2 cut(s) 836, 863
TasI AATT 7 cut(s) 147, 188, 551, 663, 930, 996, 1075
TfiI GAWTC 2 cut(s) 224, 833
Tru1I TTAA 3 cut(s) 74, 596, 731
Tru9I TTAA 3 cut(s) 74, 596, 731
TscAI CASTG 4 cut(s) 208, 373, 618, 697
TseFI GTSAC 1 cut(s) 808
TseI GCWGC 6 cut(s) 259, 280, 292, 695, 710, 821
Tsp45I GTSAC 1 cut(s) 808
TspDTI ATGAA 3 cut(s) 564, 1008, 1098
TspRI CASTG 4 cut(s) 208, 373, 618, 697
VpaK11BI GGWCC 3 cut(s) 379, 892, 942
XapI RAATTY 1 cut(s) 663
XcmI CCANNNNNNNNNTGG 1 cut(s) 463
XmiI GTMKAC 2 cut(s) 849, 863
XspI CTAG 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.