RLG00000030770

Belongs to the precorrin methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002225
Physical Location & Seq
Forward (+)
67137728 .. 67141431
3704 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030770

Sequence Viewer

Length: 1149 bp
ATGGCTCTTGTCAGTAGGCTTCAATCTCTGTCTTCCTCTTTGTCTTCCACCCATTTCAGAAAACCCAATTCCTTAAACCCTCAACCCATTTGCTCTCTACACTTCAACAACTCCCCTTCTTACACTTCTCCTTTTACAGAGAAGCATTCTATTGAGAGGTACCAGAGAGACCAATGGCTATACAATAACCAATTGGAGCAACCCACTCTTTTCTCTCTCCCTCCTGATGCTGATGCTGATGCTGATGCTGATGCTGATTCTGATTTCACAAGGCAGAATGACATTGCTTTGCAGCTACCAGAGCTGAGAAAGCTGCTTCAGGTGCTAAGAGAGAAGAGAGAGAGGGAAGGAGGGTGTGGTGGAGGAGGCAAGTGTGGGCCTGGCAATGTCTTCTTGGTCGGCACTGGCCCTGGGGACCCTGAACTCCTCACATTGAAGGCTGTCAGAGTTGTACAGAGTGCTGATTTATTGTTGTATGATAGGTTGGTGTCCAATCATGTGTTGGATTTTGTTGCCTCTGATGCTAGACTTGTCTATGTGGGCAAGACTGCTGGGTACCATAGCAGAACCCAGGAGGAGATACATGAACTGCTACTGAGTTTTGCTGAAGCTGGAGCTACTGTTGTGAGACTAAAAGGAGGGGATCCACTGGTGTTTGGAAGGGGTGGAGAGGAGATGGATTTTTTGCAAAAGCAAGGAATTCAAGTGCAAGTTATTCCAGGTATCACTGCTGCTTCTGGGATAGCAGCAGAGCTAGGGATCCCATTAACTCACAGAGGTATTGCAAATAGTGTAAGATTTCTCACCGGGCACTCCAGGAAGGGAGGAACAGACCCTCTTTTTGTGGCAGAGAATGCAGCTGACCCTGATTCAACCTTGGTGGTATACATGGGTTTGTCAACTCTCCCTTCTCTTGCTCAAAAGTTGGTCCATCATGGTCTGCCACCAACAACACCAGCTGTTGCAGTTGAGCGAGGGACCACACCTCAACAGCGCATGGTCTTTGCAGAACTAAAGGATCTTGCCAATGAAATTACATTAGCAGAGTTAGTATCACCAACGCTCATCGTCATTGGGAGAGTAGTTGCACTTTCACCATTGTGGCCGCATACTTTGAATGAAGTGGCCGCTCTCGTCGGAGCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120
Pfam Domains
Protein Families
TP_methylase

Protein Analysis

383

Amino Acids

41.2

Weight (kDa)

5.78

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 130 - 342 1.4e-51 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 159, 555
AccB1I GGYRCC 2 cut(s) 159, 555
AccBSI CCGCTC 1 cut(s) 1130
AccI GTMKAC 1 cut(s) 885
AciI CCGC 2 cut(s) 1106, 1128
AclWI GGATC 5 cut(s) 638, 651, 754, 767, 1026
AcoI YGGCCR 2 cut(s) 1103, 1125
AcsI RAATTY 1 cut(s) 699
AcuI CTGAAG 2 cut(s) 302, 627
AfaI GTAC 3 cut(s) 161, 453, 557
AgsI TTSAA 6 cut(s) 23, 106, 436, 704, 873, 1117
AjnI CCWGG 5 cut(s) 379, 409, 570, 718, 815
AleI CACNNNNGTG 1 cut(s) 1099
AluBI AGCT 8 cut(s) 295, 304, 313, 611, 617, 754, 860, 959
AluI AGCT 8 cut(s) 295, 304, 313, 611, 617, 754, 860, 959
Alw26I GTCTC 2 cut(s) 162, 622
AlwI GGATC 5 cut(s) 638, 651, 754, 767, 1026
AoxI GGCC 4 cut(s) 377, 406, 1103, 1125
ApeKI GCWGC 5 cut(s) 292, 313, 731, 746, 857
ApoI RAATTY 1 cut(s) 699
ArsI GACNNNNNNTTYG 2 cut(s) 824, 856
Asp718I GGTACC 2 cut(s) 159, 555
AspLEI GCGC 1 cut(s) 996
AspS9I GGNCC 5 cut(s) 377, 407, 415, 928, 978
AsuC2I CCSGG 1 cut(s) 808
AsuHPI GGTGA 3 cut(s) 796, 1047, 1086
AvaII GGWCC 3 cut(s) 415, 928, 978
BaeGI GKGCMC 1 cut(s) 813
BamHI GGATCC 2 cut(s) 643, 759
BanI GGYRCC 2 cut(s) 159, 555
BbsI GAAGAC 3 cut(s) 24, 36, 382
BbvI GCAGC 5 cut(s) 300, 304, 718, 758, 869
BccI CCATC 2 cut(s) 670, 939
BciT130I CCWGG 5 cut(s) 381, 411, 572, 720, 817
BcnI CCSGG 1 cut(s) 808
BcoDI GTCTC 2 cut(s) 162, 622
BfaI CTAG 2 cut(s) 525, 755
BisI GCNGC 7 cut(s) 293, 314, 732, 747, 858, 1106, 1128
BlsI GCNGC 7 cut(s) 294, 315, 733, 748, 859, 1107, 1129
Bme1390I CCNGG 6 cut(s) 381, 411, 572, 720, 808, 817
Bme18I GGWCC 3 cut(s) 415, 928, 978
BmgT120I GGNCC 5 cut(s) 377, 407, 415, 928, 978
BmiI GGNNCC 8 cut(s) 161, 416, 417, 557, 645, 761, 979, 1141
BmrFI CCNGG 6 cut(s) 381, 411, 572, 720, 808, 817
BmsI GCATC 6 cut(s) 217, 223, 229, 235, 241, 511
BpiI GAAGAC 3 cut(s) 24, 36, 382
BpmI CTGGAG 2 cut(s) 633, 799
BpuMI CCSGG 1 cut(s) 808
BsaI GGTCTC 1 cut(s) 162
BsaJI CCNNGG 4 cut(s) 409, 410, 570, 876
BsaXI ACNNNNNCTCC 2 cut(s) 606, 636
Bse1I ACTGG 2 cut(s) 409, 654
Bse3DI GCAATG 2 cut(s) 282, 391
BseBI CCWGG 5 cut(s) 381, 411, 572, 720, 817
BseDI CCNNGG 4 cut(s) 409, 410, 570, 876
BseMI GCAATG 2 cut(s) 282, 391
BseMII CTCAG 2 cut(s) 296, 587
BseNI ACTGG 2 cut(s) 409, 654
BseRI GAGGAG 4 cut(s) 378, 416, 590, 686
BseSI GKGCMC 1 cut(s) 813
BseXI GCAGC 5 cut(s) 300, 304, 718, 758, 869
BseYI CCCAGC 1 cut(s) 551
BshFI GGCC 4 cut(s) 379, 408, 1105, 1127
BshNI GGYRCC 2 cut(s) 159, 555
BsiSI CCGG 1 cut(s) 807
BslFI GGGAC 2 cut(s) 428, 991
BsmAI GTCTC 2 cut(s) 162, 622
BsmFI GGGAC 2 cut(s) 428, 991
BsmI GAATGC 2 cut(s) 145, 859
BsnI GGCC 4 cut(s) 379, 408, 1105, 1127
Bso31I GGTCTC 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 813
Bsp1407I TGTACA 1 cut(s) 451
Bsp143I GATC 3 cut(s) 643, 759, 1018
BspACI CCGC 2 cut(s) 1106, 1128
BspANI GGCC 4 cut(s) 379, 408, 1105, 1127
BspCNI CTCAG 2 cut(s) 297, 588
BspLI GGNNCC 8 cut(s) 161, 416, 417, 557, 645, 761, 979, 1141
BspPI GGATC 5 cut(s) 638, 651, 754, 767, 1026
BspT107I GGYRCC 2 cut(s) 159, 555
BspTNI GGTCTC 1 cut(s) 162
BsrBI CCGCTC 1 cut(s) 1130
BsrDI GCAATG 2 cut(s) 282, 391
BsrGI TGTACA 1 cut(s) 451
BsrI ACTGG 2 cut(s) 409, 654
BssECI CCNNGG 4 cut(s) 409, 410, 570, 876
BssMI GATC 3 cut(s) 643, 759, 1018
BssNAI GTATAC 1 cut(s) 886
BssT1I CCWWGG 1 cut(s) 876
Bst1107I GTATAC 1 cut(s) 886
Bst2UI CCWGG 5 cut(s) 381, 411, 572, 720, 817
Bst4CI ACNGT 1 cut(s) 622
Bst6I CTCTTC 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 854
BstAUI TGTACA 1 cut(s) 451
BstDEI CTNAG 3 cut(s) 305, 326, 596
BstHHI GCGC 1 cut(s) 996
BstKTI GATC 3 cut(s) 646, 762, 1021
BstMAI GTCTC 2 cut(s) 162, 622
BstMBI GATC 3 cut(s) 643, 759, 1018
BstMWI GCNNNNNNNGC 5 cut(s) 301, 310, 322, 521, 854
BstNI CCWGG 5 cut(s) 381, 411, 572, 720, 817
BstSCI CCNGG 6 cut(s) 379, 409, 570, 718, 806, 815
BstSLI GKGCMC 1 cut(s) 813
BstV1I GCAGC 5 cut(s) 300, 304, 718, 758, 869
BstV2I GAAGAC 3 cut(s) 24, 36, 382
BstX2I RGATCY 3 cut(s) 643, 759, 1018
BstYI RGATCY 3 cut(s) 643, 759, 1018
BstZ17I GTATAC 1 cut(s) 886
BsuRI GGCC 4 cut(s) 379, 408, 1105, 1127
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 3 cut(s) 402, 647, 726
CfoI GCGC 1 cut(s) 996
Cfr13I GGNCC 5 cut(s) 377, 407, 415, 928, 978
Csp6I GTAC 3 cut(s) 160, 452, 556
CspCI CAANNNNNGTGG 2 cut(s) 861, 896
CviAII CATG 5 cut(s) 497, 584, 889, 935, 997
CviQI GTAC 3 cut(s) 160, 452, 556
DdeI CTNAG 3 cut(s) 305, 326, 596
DpnI GATC 3 cut(s) 645, 761, 1020
DpnII GATC 3 cut(s) 643, 759, 1018
EaeI YGGCCR 2 cut(s) 1103, 1125
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
Eco130I CCWWGG 1 cut(s) 876
Eco31I GGTCTC 1 cut(s) 162
Eco47I GGWCC 3 cut(s) 415, 928, 978
Eco57I CTGAAG 2 cut(s) 302, 627
EcoO109I RGGNCCY 1 cut(s) 415
EcoRI GAATTC 1 cut(s) 699
EcoRII CCWGG 5 cut(s) 379, 409, 570, 718, 815
EcoT14I CCWWGG 1 cut(s) 876
ErhI CCWWGG 1 cut(s) 876
FaeI CATG 5 cut(s) 500, 587, 892, 938, 1000
FaqI GGGAC 2 cut(s) 428, 991
FatI CATG 5 cut(s) 496, 583, 888, 934, 996
FblI GTMKAC 1 cut(s) 885
Fnu4HI GCNGC 7 cut(s) 293, 314, 732, 747, 858, 1106, 1128
Fsp4HI GCNGC 7 cut(s) 293, 314, 732, 747, 858, 1106, 1128
FspBI CTAG 2 cut(s) 525, 755
GlaI GCGC 1 cut(s) 995
GluI GCNGC 7 cut(s) 293, 314, 732, 747, 858, 1106, 1128
GsaI CCCAGC 1 cut(s) 555
GsuI CTGGAG 2 cut(s) 633, 799
HaeIII GGCC 4 cut(s) 379, 408, 1105, 1127
HapII CCGG 1 cut(s) 807
HhaI GCGC 1 cut(s) 996
Hin1II CATG 5 cut(s) 500, 587, 892, 938, 1000
Hin6I GCGC 1 cut(s) 994
HinP1I GCGC 1 cut(s) 994
HincII GTYRAC 1 cut(s) 900
HindII GTYRAC 1 cut(s) 900
HinfI GANTC 2 cut(s) 257, 869
HpaII CCGG 1 cut(s) 807
HphI GGTGA 3 cut(s) 796, 1047, 1086
Hpy166II GTNNAC 2 cut(s) 886, 900
Hpy188I TCNGA 5 cut(s) 59, 262, 446, 520, 1139
Hpy188III TCNNGA 1 cut(s) 224
Hpy8I GTNNAC 2 cut(s) 886, 900
Hpy99I CGWCG 1 cut(s) 1139
HpyAV CCTTC 6 cut(s) 126, 341, 430, 654, 814, 918
HpyCH4III ACNGT 1 cut(s) 622
HpyCH4V TGCA 8 cut(s) 292, 688, 709, 785, 857, 965, 1007, 1088
HpyF10VI GCNNNNNNNGC 5 cut(s) 301, 310, 322, 521, 854
HpyF3I CTNAG 3 cut(s) 305, 326, 596
Hsp92II CATG 5 cut(s) 500, 587, 892, 938, 1000
HspAI GCGC 1 cut(s) 994
KflI GGGWCCC 1 cut(s) 415
KpnI GGTACC 2 cut(s) 163, 559
Kzo9I GATC 3 cut(s) 643, 759, 1018
LmnI GCTCC 3 cut(s) 196, 614, 1139
Lsp1109I GCAGC 5 cut(s) 300, 304, 718, 758, 869
LweI GCATC 6 cut(s) 217, 223, 229, 235, 241, 511
MaeI CTAG 2 cut(s) 525, 755
MalI GATC 3 cut(s) 645, 761, 1020
MbiI CCGCTC 1 cut(s) 1130
MboI GATC 3 cut(s) 643, 759, 1018
MboII GAAGA 4 cut(s) 24, 36, 346, 382
MfeI CAATTG 1 cut(s) 191
MflI RGATCY 3 cut(s) 643, 759, 1018
MhlI GDGCHC 1 cut(s) 813
MluCI AATT 4 cut(s) 67, 191, 699, 1032
MmeI TCCRAC 2 cut(s) 483, 1117
MseI TTAA 2 cut(s) 74, 767
MslI CAYNNNNRTG 1 cut(s) 1099
MspA1I CMGCKG 2 cut(s) 860, 959
MspI CCGG 1 cut(s) 807
MspR9I CCNGG 6 cut(s) 381, 411, 572, 720, 808, 817
MunI CAATTG 1 cut(s) 191
Mva1269I GAATGC 2 cut(s) 145, 859
MvaI CCWGG 5 cut(s) 381, 411, 572, 720, 817
MwoI GCNNNNNNNGC 5 cut(s) 301, 310, 322, 521, 854
NciI CCSGG 1 cut(s) 808
NdeII GATC 3 cut(s) 643, 759, 1018
NlaIII CATG 5 cut(s) 500, 587, 892, 938, 1000
NlaIV GGNNCC 8 cut(s) 161, 416, 417, 557, 645, 761, 979, 1141
OliI CACNNNNGTG 1 cut(s) 1099
PasI CCCWGGG 1 cut(s) 410
PctI GAATGC 2 cut(s) 145, 859
PfeI GAWTC 2 cut(s) 257, 869
PfoI TCCNGGA 1 cut(s) 815
PkrI GCNGC 7 cut(s) 294, 315, 733, 748, 859, 1107, 1129
PpuMI RGGWCCY 1 cut(s) 415
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 5 cut(s) 379, 409, 570, 718, 815
PspFI CCCAGC 1 cut(s) 551
PspGI CCWGG 5 cut(s) 379, 409, 570, 718, 815
PspN4I GGNNCC 8 cut(s) 161, 416, 417, 557, 645, 761, 979, 1141
PspPI GGNCC 5 cut(s) 377, 407, 415, 928, 978
PspPPI RGGWCCY 1 cut(s) 415
PsuI RGATCY 3 cut(s) 643, 759, 1018
PvuII CAGCTG 2 cut(s) 860, 959
RsaI GTAC 3 cut(s) 161, 453, 557
RsaNI GTAC 3 cut(s) 160, 452, 556
RseI CAYNNNNRTG 1 cut(s) 1099
SaqAI TTAA 2 cut(s) 74, 767
SatI GCNGC 7 cut(s) 293, 314, 732, 747, 858, 1106, 1128
Sau3AI GATC 3 cut(s) 643, 759, 1018
Sau96I GGNCC 5 cut(s) 377, 407, 415, 928, 978
ScrFI CCNGG 6 cut(s) 381, 411, 572, 720, 808, 817
SduI GDGCHC 1 cut(s) 813
SfaNI GCATC 6 cut(s) 217, 223, 229, 235, 241, 511
SinI GGWCC 3 cut(s) 415, 928, 978
SmiMI CAYNNNNRTG 1 cut(s) 1099
Sse9I AATT 4 cut(s) 67, 191, 699, 1032
SsiI CCGC 2 cut(s) 1106, 1128
SspMI CTAG 2 cut(s) 525, 755
StyD4I CCNGG 6 cut(s) 379, 409, 570, 718, 806, 815
StyI CCWWGG 1 cut(s) 876
TaaI ACNGT 1 cut(s) 622
TasI AATT 4 cut(s) 67, 191, 699, 1032
TatI WGTACW 1 cut(s) 451
TauI GCSGC 2 cut(s) 1108, 1130
TfiI GAWTC 2 cut(s) 257, 869
Tru1I TTAA 2 cut(s) 74, 767
Tru9I TTAA 2 cut(s) 74, 767
TscAI CASTG 3 cut(s) 409, 654, 733
TseI GCWGC 5 cut(s) 292, 313, 731, 746, 857
TspDTI ATGAA 3 cut(s) 600, 1044, 1134
TspRI CASTG 3 cut(s) 409, 654, 733
VpaK11BI GGWCC 3 cut(s) 415, 928, 978
XapI RAATTY 1 cut(s) 699
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 885
XspI CTAG 2 cut(s) 525, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.