Rmu_sc0013258.1_g000003

Belongs to the precorrin methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0013258.1
OG0002225
Physical Location & Seq
Forward (+)
17973 .. 19737
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0013258.1_g000003.1.cds

Sequence Viewer

Length: 456 bp
atgatcaaagtcttttattcctttccaggtatcactgctgcttctgggatagcagcagagctagggatcccattaactcacagaggtgttgcaaatagtgtaagatttctgaccgggcactccaggaagggaggaacggatcctctttttgtggcagagaatgcagctgaccctgattcaaccttggtggtatacatgggtttgtcgactctcccttctcttgctcaaaagttggtccatcatggtctgccaccaacaacaccagctgttgcagttgagcgagggaccacacctcaacagcgcatggtctttgcagaactgaaggatcttgccaatgaaattacattagcagagttagtatcaccaacgctcatcgtcattgggaaagtagttgcactttcaccattgtggccacatactttgaacgaagtggctgctcttgtcggagccatatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120
Pfam Domains
Protein Families
TP_methylase

Protein Analysis

151

Amino Acids

15.84

Weight (kDa)

6.5

Isoelectric Point (pI)

24.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 192, 206
AclWI GGATC 5 cut(s) 61, 74, 134, 147, 333
AcoI YGGCCR 1 cut(s) 410
AcuI CTGAAG 1 cut(s) 341
AgsI TTSAA 2 cut(s) 180, 424
AjnI CCWGG 2 cut(s) 25, 122
AleI CACNNNNGTG 2 cut(s) 84, 406
AluBI AGCT 3 cut(s) 61, 167, 266
AluI AGCT 3 cut(s) 61, 167, 266
AlwI GGATC 5 cut(s) 61, 74, 134, 147, 333
AoxI GGCC 1 cut(s) 410
ApeKI GCWGC 4 cut(s) 38, 53, 164, 434
AspLEI GCGC 1 cut(s) 303
AspS9I GGNCC 2 cut(s) 235, 285
AsuC2I CCSGG 1 cut(s) 115
AsuHPI GGTGA 2 cut(s) 354, 393
AvaII GGWCC 2 cut(s) 235, 285
BaeGI GKGCMC 1 cut(s) 120
BalI TGGCCA 1 cut(s) 412
BamHI GGATCC 2 cut(s) 66, 139
BbvI GCAGC 4 cut(s) 25, 65, 176, 421
BccI CCATC 1 cut(s) 246
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 2 cut(s) 27, 124
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 115
BfaI CTAG 1 cut(s) 62
BisI GCNGC 4 cut(s) 39, 54, 165, 435
BlsI GCNGC 4 cut(s) 40, 55, 166, 436
Bme1390I CCNGG 3 cut(s) 27, 115, 124
Bme18I GGWCC 2 cut(s) 235, 285
BmgT120I GGNCC 2 cut(s) 235, 285
BmiI GGNNCC 4 cut(s) 68, 141, 286, 448
BmrFI CCNGG 3 cut(s) 27, 115, 124
BpmI CTGGAG 1 cut(s) 106
BpuMI CCSGG 1 cut(s) 115
BsaJI CCNNGG 1 cut(s) 183
BseBI CCWGG 2 cut(s) 27, 124
BseDI CCNNGG 1 cut(s) 183
BseSI GKGCMC 1 cut(s) 120
BseXI GCAGC 4 cut(s) 25, 65, 176, 421
BshFI GGCC 1 cut(s) 412
BsiSI CCGG 1 cut(s) 114
BslFI GGGAC 1 cut(s) 298
BsmFI GGGAC 1 cut(s) 298
BsmI GAATGC 1 cut(s) 166
BsnI GGCC 1 cut(s) 412
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 4 cut(s) 3, 66, 139, 325
BspANI GGCC 1 cut(s) 412
BspLI GGNNCC 4 cut(s) 68, 141, 286, 448
BspPI GGATC 5 cut(s) 61, 74, 134, 147, 333
BssECI CCNNGG 1 cut(s) 183
BssMI GATC 4 cut(s) 3, 66, 139, 325
BssNAI GTATAC 1 cut(s) 193
BssT1I CCWWGG 1 cut(s) 183
Bst1107I GTATAC 1 cut(s) 193
Bst2UI CCWGG 2 cut(s) 27, 124
BstAPI GCANNNNNTGC 1 cut(s) 161
BstHHI GCGC 1 cut(s) 303
BstKTI GATC 4 cut(s) 6, 69, 142, 328
BstMBI GATC 4 cut(s) 3, 66, 139, 325
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstNI CCWGG 2 cut(s) 27, 124
BstSCI CCNGG 3 cut(s) 25, 113, 122
BstSLI GKGCMC 1 cut(s) 120
BstV1I GCAGC 4 cut(s) 25, 65, 176, 421
BstX2I RGATCY 3 cut(s) 66, 139, 325
BstYI RGATCY 3 cut(s) 66, 139, 325
BstZ17I GTATAC 1 cut(s) 193
BsuRI GGCC 1 cut(s) 412
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 1 cut(s) 33
CfoI GCGC 1 cut(s) 303
Cfr13I GGNCC 2 cut(s) 235, 285
CspCI CAANNNNNGTGG 4 cut(s) 168, 203, 402, 437
CviAII CATG 3 cut(s) 196, 242, 304
CviJI RGCY 6 cut(s) 61, 167, 266, 412, 434, 449
CviKI_1 RGCY 6 cut(s) 61, 167, 266, 412, 434, 449
DpnI GATC 4 cut(s) 5, 68, 141, 327
DpnII GATC 4 cut(s) 3, 66, 139, 325
EaeI YGGCCR 1 cut(s) 410
Eco130I CCWWGG 1 cut(s) 183
Eco47I GGWCC 2 cut(s) 235, 285
Eco57I CTGAAG 1 cut(s) 341
EcoRII CCWGG 2 cut(s) 25, 122
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 3 cut(s) 199, 245, 307
FaiI YATR 7 cut(s) 193, 197, 243, 305, 417, 452, 454
FaqI GGGAC 1 cut(s) 298
FatI CATG 3 cut(s) 195, 241, 303
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 2 cut(s) 192, 206
Fnu4HI GCNGC 4 cut(s) 39, 54, 165, 435
Fsp4HI GCNGC 4 cut(s) 39, 54, 165, 435
FspBI CTAG 1 cut(s) 62
GlaI GCGC 1 cut(s) 302
GluI GCNGC 4 cut(s) 39, 54, 165, 435
GsuI CTGGAG 1 cut(s) 106
HaeIII GGCC 1 cut(s) 412
HapII CCGG 1 cut(s) 114
HhaI GCGC 1 cut(s) 303
Hin1II CATG 3 cut(s) 199, 245, 307
Hin6I GCGC 1 cut(s) 301
HinP1I GCGC 1 cut(s) 301
HincII GTYRAC 1 cut(s) 207
HindII GTYRAC 1 cut(s) 207
HinfI GANTC 2 cut(s) 176, 208
HpaII CCGG 1 cut(s) 114
HphI GGTGA 2 cut(s) 354, 393
Hpy166II GTNNAC 2 cut(s) 193, 207
Hpy188I TCNGA 2 cut(s) 111, 446
Hpy8I GTNNAC 2 cut(s) 193, 207
HpyAV CCTTC 3 cut(s) 121, 225, 316
HpyCH4V TGCA 5 cut(s) 92, 164, 272, 314, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
Hsp92II CATG 3 cut(s) 199, 245, 307
HspAI GCGC 1 cut(s) 301
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 66, 139, 325
LmnI GCTCC 1 cut(s) 446
LpnPI CCDG 8 cut(s) 12, 30, 39, 109, 127, 136, 186, 276
Lsp1109I GCAGC 4 cut(s) 25, 65, 176, 421
MaeI CTAG 1 cut(s) 62
MalI GATC 4 cut(s) 5, 68, 141, 327
MboI GATC 4 cut(s) 3, 66, 139, 325
MflI RGATCY 3 cut(s) 66, 139, 325
MhlI GDGCHC 1 cut(s) 120
MlsI TGGCCA 1 cut(s) 412
MluCI AATT 1 cut(s) 339
MluNI TGGCCA 1 cut(s) 412
MlyI GAGTC 1 cut(s) 202
MmeI TCCRAC 1 cut(s) 424
MnlI CCTC 5 cut(s) 77, 125, 153, 275, 303
Mox20I TGGCCA 1 cut(s) 412
MscI TGGCCA 1 cut(s) 412
MseI TTAA 1 cut(s) 74
MslI CAYNNNNRTG 2 cut(s) 84, 406
Msp20I TGGCCA 1 cut(s) 412
MspA1I CMGCKG 2 cut(s) 167, 266
MspI CCGG 1 cut(s) 114
MspR9I CCNGG 3 cut(s) 27, 115, 124
Mva1269I GAATGC 1 cut(s) 166
MvaI CCWGG 2 cut(s) 27, 124
MwoI GCNNNNNNNGC 1 cut(s) 161
NciI CCSGG 1 cut(s) 115
NdeII GATC 4 cut(s) 3, 66, 139, 325
NlaIII CATG 3 cut(s) 199, 245, 307
NlaIV GGNNCC 4 cut(s) 68, 141, 286, 448
OliI CACNNNNGTG 2 cut(s) 84, 406
PctI GAATGC 1 cut(s) 166
PfeI GAWTC 1 cut(s) 176
PfoI TCCNGGA 1 cut(s) 122
PkrI GCNGC 4 cut(s) 40, 55, 166, 436
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
Psp6I CCWGG 2 cut(s) 25, 122
PspGI CCWGG 2 cut(s) 25, 122
PspN4I GGNNCC 4 cut(s) 68, 141, 286, 448
PspPI GGNCC 2 cut(s) 235, 285
PsuI RGATCY 3 cut(s) 66, 139, 325
PvuII CAGCTG 2 cut(s) 167, 266
RseI CAYNNNNRTG 2 cut(s) 84, 406
SalI GTCGAC 1 cut(s) 205
SaqAI TTAA 1 cut(s) 74
SatI GCNGC 4 cut(s) 39, 54, 165, 435
Sau3AI GATC 4 cut(s) 3, 66, 139, 325
Sau96I GGNCC 2 cut(s) 235, 285
SchI GAGTC 1 cut(s) 202
ScrFI CCNGG 3 cut(s) 27, 115, 124
SduI GDGCHC 1 cut(s) 120
SetI ASST 7 cut(s) 31, 63, 88, 169, 185, 268, 295
SinI GGWCC 2 cut(s) 235, 285
SmiMI CAYNNNNRTG 2 cut(s) 84, 406
Sse9I AATT 1 cut(s) 339
SspMI CTAG 1 cut(s) 62
StyD4I CCNGG 3 cut(s) 25, 113, 122
StyI CCWWGG 1 cut(s) 183
TaqI TCGA 1 cut(s) 206
TasI AATT 1 cut(s) 339
TfiI GAWTC 1 cut(s) 176
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TscAI CASTG 1 cut(s) 40
TseI GCWGC 4 cut(s) 38, 53, 164, 434
TspDTI ATGAA 1 cut(s) 351
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 1 cut(s) 40
VpaK11BI GGWCC 2 cut(s) 235, 285
XmiI GTMKAC 2 cut(s) 192, 206
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.