RLG00000013272

Belongs to the precorrin methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002225
Physical Location & Seq
Forward (+)
29026563 .. 29030593
4031 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013272

Sequence Viewer

Length: 1176 bp
ATGGCTCTTGTGAGTAGGCTTCAATCTCTGTCTTCCACCCATTTCAGAAAACCCAATTGCTTAAACCCTCAACCCATTTGCTCTCTACACTTCAACTCCCCTTCTTACACTTCTCCTTTTACAGAGAAGCATTCTATTGAGAGGTACCAGAGAGACCGGTGGCTATACAATAACCAATTGGAGCAACCCACTCTAATCTCTCTCCCTCCTGATTCTGATTCTGATTTCACAAGGCAGAATGACATTGCTTTGCAGCTACCAGAGCTGAGAAAGCTGCTTCAGGTGCTGCGAGAGAAGAGAGAGAGGGAAGGAGGGTGTGGTGGAGGCAAGTGTGGGCCTGGCAATGTCTTCTTGGTGGGCACTGGCCCTGGGGACCCTGAACTCCTGACATTGAAGGCTGTCAGAGTTATTCAGAGTGCTGATTTATTGTTGTATGATAGGTTGGTGTCCAATCATGTGTTGGATTTTGTTGCCTCTGATGCTAGACTTGTCTATGTGGGCAAGACTGCTGGGTACCATAGCAGAACCCAGGAGGAGATACATGAATTGCTACTGAGTTTTGCTGAAGCCGGAGCTACTGTTGTGAGATTAAAAGGAGGGGATCCACTGGTGTTTGGAAGGGGTGGAGAGGAGATGGATTTTTTGCAACAGCAAGGAATTCAAGTGCAAGTTATTCCTGGTATCACTACTGCTTCTGGGATAGCAGCAGAGCTTGGGATCCCATTAACTCATAGAGGTGTTGCAAATAGCGTAAGATTTCTGACCGGGCACTCCAGGAAGGGAGGAACAGATCCTCTTTTTGTGACAGAGAATGCAGCTGACCCTGATTCGACCTTGGTGGTATACATGGGCTTGTTGACTCTCCCTTCTCTTGCTCAAAAGTTGGTCCATCATGGTCTGCCACCAACAACACCAGCTGTTGCAATTGAGCGAGGGACCACACCTCAACAGCGCATGGTCTTTGCAGAACTGAAGGATCTTGCCAATGAAATTACATCAGCAGAGTTAGTATCACCAACGCTCATCGTCATTGGGAAAACAGAAAAACGAGCCTTTTGTTCTGATGTTGTTTCAATCGAAACCGAGGCTTTCATGGTCGTGTGTGAATTGGTGGTTCCTTCTTTGGTTACCGGGCGCATGAGGAAGTTGAAACTACAGATCATTTCATCATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120
Pfam Domains
Protein Families
TP_methylase

Protein Analysis

392

Amino Acids

42.74

Weight (kDa)

6.57

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 116 - 328 9.3e-51 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1174
Acc65I GGTACC 2 cut(s) 144, 513
AccB1I GGYRCC 2 cut(s) 144, 513
AccI GTMKAC 1 cut(s) 843
AclWI GGATC 6 cut(s) 596, 609, 712, 725, 785, 984
AcsI RAATTY 1 cut(s) 657
AcuI CTGAAG 3 cut(s) 263, 585, 992
AfaI GTAC 2 cut(s) 146, 515
AgeI ACCGGT 1 cut(s) 156
AgsI TTSAA 6 cut(s) 23, 94, 394, 662, 1074, 1150
AjnI CCWGG 5 cut(s) 337, 367, 528, 676, 773
AluBI AGCT 7 cut(s) 256, 265, 274, 575, 712, 818, 917
AluI AGCT 7 cut(s) 256, 265, 274, 575, 712, 818, 917
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 6 cut(s) 596, 609, 712, 725, 785, 984
AlwNI CAGNNNCTG 1 cut(s) 286
AoxI GGCC 2 cut(s) 335, 364
ApeKI GCWGC 5 cut(s) 253, 274, 286, 704, 815
ApoI RAATTY 1 cut(s) 657
AsiGI ACCGGT 1 cut(s) 156
Asp718I GGTACC 2 cut(s) 144, 513
AspLEI GCGC 2 cut(s) 954, 1137
AspS9I GGNCC 5 cut(s) 335, 365, 373, 886, 936
AsuC2I CCSGG 2 cut(s) 766, 1132
AsuHPI GGTGA 1 cut(s) 1005
AvaII GGWCC 3 cut(s) 373, 886, 936
BaeGI GKGCMC 2 cut(s) 362, 771
BamHI GGATCC 2 cut(s) 601, 717
BanI GGYRCC 2 cut(s) 144, 513
BbsI GAAGAC 2 cut(s) 24, 340
BbvI GCAGC 5 cut(s) 261, 265, 273, 716, 827
BccI CCATC 2 cut(s) 628, 897
BciT130I CCWGG 5 cut(s) 339, 369, 530, 678, 775
BcnI CCSGG 2 cut(s) 766, 1132
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 1 cut(s) 483
BfmI CTRYAG 1 cut(s) 1154
BisI GCNGC 5 cut(s) 254, 275, 287, 705, 816
BlsI GCNGC 5 cut(s) 255, 276, 288, 706, 817
Bme1390I CCNGG 7 cut(s) 339, 369, 530, 678, 766, 775, 1132
Bme18I GGWCC 3 cut(s) 373, 886, 936
BmgT120I GGNCC 5 cut(s) 335, 365, 373, 886, 936
BmiI GGNNCC 8 cut(s) 146, 374, 375, 515, 603, 719, 937, 1116
BmrFI CCNGG 7 cut(s) 339, 369, 530, 678, 766, 775, 1132
BmsI GCATC 1 cut(s) 469
BpiI GAAGAC 2 cut(s) 24, 340
BpmI CTGGAG 1 cut(s) 757
BpuMI CCSGG 2 cut(s) 766, 1132
BsaI GGTCTC 1 cut(s) 147
BsaJI CCNNGG 5 cut(s) 367, 368, 528, 834, 1083
BsaWI WCCGGW 1 cut(s) 156
BsaXI ACNNNNNCTCC 4 cut(s) 80, 110, 564, 594
Bse118I RCCGGY 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 367, 612
Bse3DI GCAATG 2 cut(s) 243, 349
BseBI CCWGG 5 cut(s) 339, 369, 530, 678, 775
BseDI CCNNGG 5 cut(s) 367, 368, 528, 834, 1083
BseMI GCAATG 2 cut(s) 243, 349
BseMII CTCAG 2 cut(s) 257, 545
BseNI ACTGG 2 cut(s) 367, 612
BseRI GAGGAG 2 cut(s) 548, 644
BseSI GKGCMC 2 cut(s) 362, 771
BseXI GCAGC 5 cut(s) 261, 265, 273, 716, 827
BseYI CCCAGC 1 cut(s) 509
BshFI GGCC 2 cut(s) 337, 366
BshNI GGYRCC 2 cut(s) 144, 513
BshTI ACCGGT 1 cut(s) 156
BsiSI CCGG 4 cut(s) 157, 570, 765, 1131
BslFI GGGAC 2 cut(s) 386, 949
BsmAI GTCTC 1 cut(s) 147
BsmFI GGGAC 2 cut(s) 386, 949
BsmI GAATGC 2 cut(s) 130, 817
BsnI GGCC 2 cut(s) 337, 366
Bso31I GGTCTC 1 cut(s) 147
Bsp1286I GDGCHC 2 cut(s) 362, 771
Bsp143I GATC 5 cut(s) 601, 717, 790, 976, 1158
BspANI GGCC 2 cut(s) 337, 366
BspCNI CTCAG 2 cut(s) 258, 546
BspLI GGNNCC 8 cut(s) 146, 374, 375, 515, 603, 719, 937, 1116
BspPI GGATC 6 cut(s) 596, 609, 712, 725, 785, 984
BspT107I GGYRCC 2 cut(s) 144, 513
BspTNI GGTCTC 1 cut(s) 147
BsrDI GCAATG 2 cut(s) 243, 349
BsrFI RCCGGY 1 cut(s) 156
BsrI ACTGG 2 cut(s) 367, 612
BssAI RCCGGY 1 cut(s) 156
BssECI CCNNGG 5 cut(s) 367, 368, 528, 834, 1083
BssMI GATC 5 cut(s) 601, 717, 790, 976, 1158
BssNAI GTATAC 1 cut(s) 844
BssT1I CCWWGG 1 cut(s) 834
Bst1107I GTATAC 1 cut(s) 844
Bst2UI CCWGG 5 cut(s) 339, 369, 530, 678, 775
Bst4CI ACNGT 1 cut(s) 580
Bst6I CTCTTC 1 cut(s) 290
BstDEI CTNAG 2 cut(s) 266, 554
BstEII GGTNACC 1 cut(s) 1126
BstHHI GCGC 2 cut(s) 954, 1137
BstKTI GATC 5 cut(s) 604, 720, 793, 979, 1161
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 5 cut(s) 601, 717, 790, 976, 1158
BstMWI GCNNNNNNNGC 4 cut(s) 262, 271, 283, 479
BstNI CCWGG 5 cut(s) 339, 369, 530, 678, 775
BstPI GGTNACC 1 cut(s) 1126
BstSCI CCNGG 7 cut(s) 337, 367, 528, 676, 764, 773, 1130
BstSFI CTRYAG 1 cut(s) 1154
BstSLI GKGCMC 2 cut(s) 362, 771
BstV1I GCAGC 5 cut(s) 261, 265, 273, 716, 827
BstV2I GAAGAC 2 cut(s) 24, 340
BstX2I RGATCY 4 cut(s) 601, 717, 790, 976
BstYI RGATCY 4 cut(s) 601, 717, 790, 976
BstZ17I GTATAC 1 cut(s) 844
BsuRI GGCC 2 cut(s) 337, 366
BtsIMutI CAGTG 2 cut(s) 360, 605
CaiI CAGNNNCTG 1 cut(s) 286
CfoI GCGC 2 cut(s) 954, 1137
Cfr10I RCCGGY 1 cut(s) 156
Cfr13I GGNCC 5 cut(s) 335, 365, 373, 886, 936
Csp6I GTAC 2 cut(s) 145, 514
CspAI ACCGGT 1 cut(s) 156
CviAII CATG 7 cut(s) 455, 542, 847, 893, 955, 1093, 1138
CviQI GTAC 2 cut(s) 145, 514
DdeI CTNAG 2 cut(s) 266, 554
DpnI GATC 5 cut(s) 603, 719, 792, 978, 1160
DpnII GATC 5 cut(s) 601, 717, 790, 976, 1158
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 834
Eco31I GGTCTC 1 cut(s) 147
Eco47I GGWCC 3 cut(s) 373, 886, 936
Eco57I CTGAAG 3 cut(s) 263, 585, 992
Eco91I GGTNACC 1 cut(s) 1126
EcoO109I RGGNCCY 1 cut(s) 373
EcoO65I GGTNACC 1 cut(s) 1126
EcoRI GAATTC 1 cut(s) 657
EcoRII CCWGG 5 cut(s) 337, 367, 528, 676, 773
EcoT14I CCWWGG 1 cut(s) 834
ErhI CCWWGG 1 cut(s) 834
FaeI CATG 7 cut(s) 458, 545, 850, 896, 958, 1096, 1141
FaqI GGGAC 2 cut(s) 386, 949
FatI CATG 7 cut(s) 454, 541, 846, 892, 954, 1092, 1137
FblI GTMKAC 1 cut(s) 843
Fnu4HI GCNGC 5 cut(s) 254, 275, 287, 705, 816
Fsp4HI GCNGC 5 cut(s) 254, 275, 287, 705, 816
FspBI CTAG 1 cut(s) 483
GlaI GCGC 2 cut(s) 953, 1136
GluI GCNGC 5 cut(s) 254, 275, 287, 705, 816
GsaI CCCAGC 1 cut(s) 513
GsuI CTGGAG 1 cut(s) 757
HaeIII GGCC 2 cut(s) 337, 366
HapII CCGG 4 cut(s) 157, 570, 765, 1131
HhaI GCGC 2 cut(s) 954, 1137
Hin1II CATG 7 cut(s) 458, 545, 850, 896, 958, 1096, 1141
Hin6I GCGC 2 cut(s) 952, 1135
HinP1I GCGC 2 cut(s) 952, 1135
HincII GTYRAC 1 cut(s) 858
HindII GTYRAC 1 cut(s) 858
HinfI GANTC 4 cut(s) 212, 218, 827, 859
HpaII CCGG 4 cut(s) 157, 570, 765, 1131
HphI GGTGA 1 cut(s) 1005
Hpy166II GTNNAC 2 cut(s) 844, 858
Hpy188I TCNGA 8 cut(s) 47, 217, 223, 404, 414, 478, 762, 1063
Hpy188III TCNNGA 2 cut(s) 209, 385
Hpy8I GTNNAC 2 cut(s) 844, 858
HpyAV CCTTC 8 cut(s) 111, 302, 388, 612, 772, 876, 967, 1128
HpyCH4III ACNGT 1 cut(s) 580
HpyCH4V TGCA 7 cut(s) 253, 646, 667, 743, 815, 923, 965
HpyF10VI GCNNNNNNNGC 4 cut(s) 262, 271, 283, 479
HpyF3I CTNAG 2 cut(s) 266, 554
Hsp92II CATG 7 cut(s) 458, 545, 850, 896, 958, 1096, 1141
HspAI GCGC 2 cut(s) 952, 1135
KflI GGGWCCC 1 cut(s) 373
KpnI GGTACC 2 cut(s) 148, 517
Kzo9I GATC 5 cut(s) 601, 717, 790, 976, 1158
LmnI GCTCC 2 cut(s) 181, 572
Lsp1109I GCAGC 5 cut(s) 261, 265, 273, 716, 827
LweI GCATC 1 cut(s) 469
MaeI CTAG 1 cut(s) 483
MaeIII GTNAC 2 cut(s) 802, 1126
MalI GATC 5 cut(s) 603, 719, 792, 978, 1160
MboI GATC 5 cut(s) 601, 717, 790, 976, 1158
MboII GAAGA 3 cut(s) 24, 307, 340
MfeI CAATTG 3 cut(s) 55, 176, 924
MflI RGATCY 4 cut(s) 601, 717, 790, 976
MhlI GDGCHC 2 cut(s) 362, 771
MluCI AATT 7 cut(s) 55, 176, 545, 657, 924, 990, 1106
MlyI GAGTC 1 cut(s) 853
MmeI TCCRAC 1 cut(s) 441
MseI TTAA 3 cut(s) 62, 590, 725
MslI CAYNNNNRTG 2 cut(s) 735, 1097
MspA1I CMGCKG 2 cut(s) 818, 917
MspI CCGG 4 cut(s) 157, 570, 765, 1131
MspR9I CCNGG 7 cut(s) 339, 369, 530, 678, 766, 775, 1132
MunI CAATTG 3 cut(s) 55, 176, 924
Mva1269I GAATGC 2 cut(s) 130, 817
MvaI CCWGG 5 cut(s) 339, 369, 530, 678, 775
MwoI GCNNNNNNNGC 4 cut(s) 262, 271, 283, 479
NciI CCSGG 2 cut(s) 766, 1132
NdeII GATC 5 cut(s) 601, 717, 790, 976, 1158
NlaIII CATG 7 cut(s) 458, 545, 850, 896, 958, 1096, 1141
NlaIV GGNNCC 8 cut(s) 146, 374, 375, 515, 603, 719, 937, 1116
NmuCI GTSAC 1 cut(s) 802
PasI CCCWGGG 1 cut(s) 368
PctI GAATGC 2 cut(s) 130, 817
PfeI GAWTC 3 cut(s) 212, 218, 827
PfoI TCCNGGA 1 cut(s) 773
PinAI ACCGGT 1 cut(s) 156
PkrI GCNGC 5 cut(s) 255, 276, 288, 706, 817
PleI GAGTC 1 cut(s) 853
PpsI GAGTC 1 cut(s) 853
PpuMI RGGWCCY 1 cut(s) 373
PsiI TTATAA 1 cut(s) 1174
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 5 cut(s) 337, 367, 528, 676, 773
PspEI GGTNACC 1 cut(s) 1126
PspFI CCCAGC 1 cut(s) 509
PspGI CCWGG 5 cut(s) 337, 367, 528, 676, 773
PspN4I GGNNCC 8 cut(s) 146, 374, 375, 515, 603, 719, 937, 1116
PspPI GGNCC 5 cut(s) 335, 365, 373, 886, 936
PspPPI RGGWCCY 1 cut(s) 373
PstNI CAGNNNCTG 1 cut(s) 286
PsuI RGATCY 4 cut(s) 601, 717, 790, 976
PvuII CAGCTG 2 cut(s) 818, 917
RsaI GTAC 2 cut(s) 146, 515
RsaNI GTAC 2 cut(s) 145, 514
RseI CAYNNNNRTG 2 cut(s) 735, 1097
SaqAI TTAA 3 cut(s) 62, 590, 725
SatI GCNGC 5 cut(s) 254, 275, 287, 705, 816
Sau3AI GATC 5 cut(s) 601, 717, 790, 976, 1158
Sau96I GGNCC 5 cut(s) 335, 365, 373, 886, 936
SchI GAGTC 1 cut(s) 853
ScrFI CCNGG 7 cut(s) 339, 369, 530, 678, 766, 775, 1132
SduI GDGCHC 2 cut(s) 362, 771
SfaNI GCATC 1 cut(s) 469
SfcI CTRYAG 1 cut(s) 1154
SinI GGWCC 3 cut(s) 373, 886, 936
SmiMI CAYNNNNRTG 2 cut(s) 735, 1097
Sse9I AATT 7 cut(s) 55, 176, 545, 657, 924, 990, 1106
SspMI CTAG 1 cut(s) 483
StyD4I CCNGG 7 cut(s) 337, 367, 528, 676, 764, 773, 1130
StyI CCWWGG 1 cut(s) 834
TaaI ACNGT 1 cut(s) 580
TaqI TCGA 2 cut(s) 830, 1077
TasI AATT 7 cut(s) 55, 176, 545, 657, 924, 990, 1106
TfiI GAWTC 3 cut(s) 212, 218, 827
Tru1I TTAA 3 cut(s) 62, 590, 725
Tru9I TTAA 3 cut(s) 62, 590, 725
TscAI CASTG 2 cut(s) 367, 612
TseFI GTSAC 1 cut(s) 802
TseI GCWGC 5 cut(s) 253, 274, 286, 704, 815
Tsp45I GTSAC 1 cut(s) 802
TspDTI ATGAA 4 cut(s) 558, 1002, 1081, 1155
TspRI CASTG 2 cut(s) 367, 612
VpaK11BI GGWCC 3 cut(s) 373, 886, 936
XapI RAATTY 1 cut(s) 657
XcmI CCANNNNNNNNNTGG 1 cut(s) 457
XmiI GTMKAC 1 cut(s) 843
XspI CTAG 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.