Rroxscaffold_7G00190980

Belongs to the precorrin methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002225
Physical Location & Seq
Forward (+)
31434535 .. 31438337
3803 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00190980.1

Sequence Viewer

Length: 1122 bp
ATGGCTCTTGTGAGTAGGCTTCAATCTCTGTCTTCCACCCATTTCAGAAAACCCAATTGCTTAAACCCTCAACCCATTTGCTCTCTACACTTGAACTCCCCTTCTTACACTTCTCCTTTTACAGAGAAGCATTCTATTGAGAGGTACCAGAGAGACCGGTGGCTATACAATAACCAATTGCAGCAACCCACTCTAATCTCTCTCCCTCCTGATTCTGATTCTGATTCTGATTTCACAAGGCAGAATGACATTGCTTTGCAGCTACCAGAGCTGAGAAAGCTGCTTCAGGTGCTGCGAGAGAAGAGAGAGAAGAGAGAGAGGGAAGGAGGGTGTGGTGGAGGTAAGTGTGGGCCTGGCAATGTCTTCTTGGTGGGCACTGGCCCTGGGGACCCTGAACTCCTGACATTGAAGGCTGTCAGAGTTATTCAGAGTGCCGATTTATTGTTGTATGATAGGTTGGTGTCCAATGATGTGTTGGATTTTGTTGCCTCTGATGCTAGACTTGTCTATGTGGGCAAGACTGCTGGGTACCATAGCAGGACCCAGGAGGAGATACATGAATTGCTACTGAGTTTTGCTGAAGCCGGAGCTACTGTTGTGAGATTAAAAGGAGGGGATCCACTGGTGTTTGGAAGGGGTGGAGAAGAGATGGATTTTTTGCAACAGCAAGGAATTCAAGTGCAAGTTATTCCTGGTATCACTGCTGCTTCTGGGATAGCAGCAGAGCTTGGGATCCCATTAACTCATAGAGGTGTTGCAAATAGTGTAAGATTTCTGACCGGGCACTCCAGGAAGGGAGGAACAGATCCTCTTTTTGTGACAGAGAATGCAGCTGACCCTGATTCGACCTTGGTGGTTTACATGGGTTTGTCGACTCTCCCTTCTCTTGCTCAAAAGTTCGTCCATCATGGTCTACCACCGACAACACCAGCTGTTGCAATTGAGCGAGGGACCACACCTCAACAGCGCATGGTCTTTGCAGAACTGAAGGATCTTGCCAATGAAATTACATCAGCAGAGTTAGTATCACCAACGCTCATCGTCATTGGGAAAGTAGTTGCACTTTCACCATTGTGGCCACATAATTTGAATGAAGTGGCCTCCCTTGTTGGAGCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120
Pfam Domains
Protein Families
TP_methylase

Protein Analysis

373

Amino Acids

40.55

Weight (kDa)

6.22

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 121 - 333 2.8e-51 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 144, 528
AccB1I GGYRCC 2 cut(s) 144, 528
AccI GTMKAC 2 cut(s) 872, 913
AclWI GGATC 6 cut(s) 611, 624, 727, 740, 800, 999
AcoI YGGCCR 1 cut(s) 1076
AcsI RAATTY 1 cut(s) 672
AcuI CTGAAG 3 cut(s) 269, 600, 1007
AfaI GTAC 2 cut(s) 146, 530
AgeI ACCGGT 1 cut(s) 156
AgsI TTSAA 5 cut(s) 23, 94, 409, 677, 1090
AjnI CCWGG 5 cut(s) 352, 382, 543, 691, 788
AleI CACNNNNGTG 1 cut(s) 1072
AluBI AGCT 7 cut(s) 262, 271, 280, 590, 727, 833, 932
AluI AGCT 7 cut(s) 262, 271, 280, 590, 727, 833, 932
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 6 cut(s) 611, 624, 727, 740, 800, 999
AlwNI CAGNNNCTG 1 cut(s) 292
AoxI GGCC 4 cut(s) 350, 379, 1076, 1098
ApeKI GCWGC 7 cut(s) 181, 259, 280, 292, 704, 719, 830
ApoI RAATTY 1 cut(s) 672
AsiGI ACCGGT 1 cut(s) 156
Asp718I GGTACC 2 cut(s) 144, 528
AspLEI GCGC 1 cut(s) 969
AspS9I GGNCC 5 cut(s) 350, 380, 388, 540, 951
AsuC2I CCSGG 1 cut(s) 781
AsuHPI GGTGA 2 cut(s) 1020, 1059
AvaII GGWCC 3 cut(s) 388, 540, 951
BaeGI GKGCMC 2 cut(s) 377, 786
BalI TGGCCA 1 cut(s) 1078
BamHI GGATCC 2 cut(s) 616, 732
BanI GGYRCC 2 cut(s) 144, 528
BbsI GAAGAC 2 cut(s) 24, 355
BbvI GCAGC 7 cut(s) 193, 267, 271, 279, 691, 731, 842
BccI CCATC 2 cut(s) 643, 912
BciT130I CCWGG 5 cut(s) 354, 384, 545, 693, 790
BcnI CCSGG 1 cut(s) 781
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 1 cut(s) 498
BisI GCNGC 7 cut(s) 182, 260, 281, 293, 705, 720, 831
BlsI GCNGC 7 cut(s) 183, 261, 282, 294, 706, 721, 832
Bme1390I CCNGG 6 cut(s) 354, 384, 545, 693, 781, 790
Bme18I GGWCC 3 cut(s) 388, 540, 951
BmgT120I GGNCC 5 cut(s) 350, 380, 388, 540, 951
BmiI GGNNCC 9 cut(s) 146, 389, 390, 530, 542, 618, 734, 952, 1114
BmrFI CCNGG 6 cut(s) 354, 384, 545, 693, 781, 790
BmsI GCATC 1 cut(s) 484
BpiI GAAGAC 2 cut(s) 24, 355
BpmI CTGGAG 1 cut(s) 772
BpuMI CCSGG 1 cut(s) 781
BsaI GGTCTC 1 cut(s) 147
BsaJI CCNNGG 4 cut(s) 382, 383, 543, 849
BsaWI WCCGGW 1 cut(s) 156
BsaXI ACNNNNNCTCC 4 cut(s) 80, 110, 579, 609
Bse118I RCCGGY 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 382, 627
Bse3DI GCAATG 2 cut(s) 249, 364
BseBI CCWGG 5 cut(s) 354, 384, 545, 693, 790
BseDI CCNNGG 4 cut(s) 382, 383, 543, 849
BseMI GCAATG 2 cut(s) 249, 364
BseMII CTCAG 2 cut(s) 263, 560
BseNI ACTGG 2 cut(s) 382, 627
BseRI GAGGAG 1 cut(s) 563
BseSI GKGCMC 2 cut(s) 377, 786
BseXI GCAGC 7 cut(s) 193, 267, 271, 279, 691, 731, 842
BseYI CCCAGC 1 cut(s) 524
BshFI GGCC 4 cut(s) 352, 381, 1078, 1100
BshNI GGYRCC 2 cut(s) 144, 528
BshTI ACCGGT 1 cut(s) 156
BsiSI CCGG 3 cut(s) 157, 585, 780
BslFI GGGAC 2 cut(s) 401, 964
BsmAI GTCTC 1 cut(s) 147
BsmFI GGGAC 2 cut(s) 401, 964
BsmI GAATGC 2 cut(s) 130, 832
BsnI GGCC 4 cut(s) 352, 381, 1078, 1100
Bso31I GGTCTC 1 cut(s) 147
Bsp1286I GDGCHC 2 cut(s) 377, 786
Bsp143I GATC 4 cut(s) 616, 732, 805, 991
BspANI GGCC 4 cut(s) 352, 381, 1078, 1100
BspCNI CTCAG 2 cut(s) 264, 561
BspLI GGNNCC 9 cut(s) 146, 389, 390, 530, 542, 618, 734, 952, 1114
BspPI GGATC 6 cut(s) 611, 624, 727, 740, 800, 999
BspT107I GGYRCC 2 cut(s) 144, 528
BspTNI GGTCTC 1 cut(s) 147
BsrDI GCAATG 2 cut(s) 249, 364
BsrFI RCCGGY 1 cut(s) 156
BsrI ACTGG 2 cut(s) 382, 627
BssAI RCCGGY 1 cut(s) 156
BssECI CCNNGG 4 cut(s) 382, 383, 543, 849
BssMI GATC 4 cut(s) 616, 732, 805, 991
BssT1I CCWWGG 1 cut(s) 849
Bst2UI CCWGG 5 cut(s) 354, 384, 545, 693, 790
Bst4CI ACNGT 1 cut(s) 595
Bst6I CTCTTC 3 cut(s) 296, 305, 639
BstDEI CTNAG 2 cut(s) 272, 569
BstHHI GCGC 1 cut(s) 969
BstKTI GATC 4 cut(s) 619, 735, 808, 994
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 4 cut(s) 616, 732, 805, 991
BstMWI GCNNNNNNNGC 4 cut(s) 268, 277, 289, 494
BstNI CCWGG 5 cut(s) 354, 384, 545, 693, 790
BstSCI CCNGG 6 cut(s) 352, 382, 543, 691, 779, 788
BstSLI GKGCMC 2 cut(s) 377, 786
BstV1I GCAGC 7 cut(s) 193, 267, 271, 279, 691, 731, 842
BstV2I GAAGAC 2 cut(s) 24, 355
BstX2I RGATCY 4 cut(s) 616, 732, 805, 991
BstYI RGATCY 4 cut(s) 616, 732, 805, 991
BsuRI GGCC 4 cut(s) 352, 381, 1078, 1100
BtsI GCAGTG 1 cut(s) 699
BtsIMutI CAGTG 3 cut(s) 375, 620, 699
CaiI CAGNNNCTG 1 cut(s) 292
CfoI GCGC 1 cut(s) 969
Cfr10I RCCGGY 1 cut(s) 156
Cfr13I GGNCC 5 cut(s) 350, 380, 388, 540, 951
Csp6I GTAC 2 cut(s) 145, 529
CspAI ACCGGT 1 cut(s) 156
CspCI CAANNNNNGTGG 2 cut(s) 1068, 1103
CviAII CATG 4 cut(s) 557, 862, 908, 970
CviQI GTAC 2 cut(s) 145, 529
DdeI CTNAG 2 cut(s) 272, 569
DpnI GATC 4 cut(s) 618, 734, 807, 993
DpnII GATC 4 cut(s) 616, 732, 805, 991
EaeI YGGCCR 1 cut(s) 1076
Eam1104I CTCTTC 3 cut(s) 296, 305, 639
EarI CTCTTC 3 cut(s) 296, 305, 639
Eco130I CCWWGG 1 cut(s) 849
Eco31I GGTCTC 1 cut(s) 147
Eco47I GGWCC 3 cut(s) 388, 540, 951
Eco57I CTGAAG 3 cut(s) 269, 600, 1007
EcoO109I RGGNCCY 2 cut(s) 388, 540
EcoRI GAATTC 1 cut(s) 672
EcoRII CCWGG 5 cut(s) 352, 382, 543, 691, 788
EcoT14I CCWWGG 1 cut(s) 849
ErhI CCWWGG 1 cut(s) 849
FaeI CATG 4 cut(s) 560, 865, 911, 973
FaqI GGGAC 2 cut(s) 401, 964
FatI CATG 4 cut(s) 556, 861, 907, 969
FblI GTMKAC 2 cut(s) 872, 913
Fnu4HI GCNGC 7 cut(s) 182, 260, 281, 293, 705, 720, 831
Fsp4HI GCNGC 7 cut(s) 182, 260, 281, 293, 705, 720, 831
FspBI CTAG 1 cut(s) 498
GlaI GCGC 1 cut(s) 968
GluI GCNGC 7 cut(s) 182, 260, 281, 293, 705, 720, 831
GsaI CCCAGC 1 cut(s) 528
GsuI CTGGAG 1 cut(s) 772
HaeIII GGCC 4 cut(s) 352, 381, 1078, 1100
HapII CCGG 3 cut(s) 157, 585, 780
HhaI GCGC 1 cut(s) 969
Hin1II CATG 4 cut(s) 560, 865, 911, 973
Hin6I GCGC 1 cut(s) 967
HinP1I GCGC 1 cut(s) 967
HincII GTYRAC 1 cut(s) 873
HindII GTYRAC 1 cut(s) 873
HinfI GANTC 5 cut(s) 212, 218, 224, 842, 874
HpaII CCGG 3 cut(s) 157, 585, 780
HphI GGTGA 2 cut(s) 1020, 1059
Hpy166II GTNNAC 3 cut(s) 859, 873, 914
Hpy188I TCNGA 8 cut(s) 47, 217, 223, 229, 419, 429, 493, 777
Hpy188III TCNNGA 2 cut(s) 209, 400
Hpy8I GTNNAC 3 cut(s) 859, 873, 914
HpyAV CCTTC 7 cut(s) 111, 317, 403, 627, 787, 891, 982
HpyCH4III ACNGT 1 cut(s) 595
HpyCH4V TGCA 9 cut(s) 181, 259, 661, 682, 758, 830, 938, 980, 1061
HpyF10VI GCNNNNNNNGC 4 cut(s) 268, 277, 289, 494
HpyF3I CTNAG 2 cut(s) 272, 569
Hsp92II CATG 4 cut(s) 560, 865, 911, 973
HspAI GCGC 1 cut(s) 967
KflI GGGWCCC 1 cut(s) 388
KpnI GGTACC 2 cut(s) 148, 532
Kzo9I GATC 4 cut(s) 616, 732, 805, 991
LmnI GCTCC 2 cut(s) 587, 1112
Lsp1109I GCAGC 7 cut(s) 193, 267, 271, 279, 691, 731, 842
LweI GCATC 1 cut(s) 484
MaeI CTAG 1 cut(s) 498
MaeIII GTNAC 1 cut(s) 817
MalI GATC 4 cut(s) 618, 734, 807, 993
MboI GATC 4 cut(s) 616, 732, 805, 991
MboII GAAGA 5 cut(s) 24, 313, 322, 355, 656
MfeI CAATTG 3 cut(s) 55, 176, 939
MflI RGATCY 4 cut(s) 616, 732, 805, 991
MhlI GDGCHC 2 cut(s) 377, 786
MlsI TGGCCA 1 cut(s) 1078
MluCI AATT 7 cut(s) 55, 176, 560, 672, 939, 1005, 1084
MluNI TGGCCA 1 cut(s) 1078
MlyI GAGTC 1 cut(s) 868
MmeI TCCRAC 2 cut(s) 456, 1090
Mox20I TGGCCA 1 cut(s) 1078
MscI TGGCCA 1 cut(s) 1078
MseI TTAA 3 cut(s) 62, 605, 740
MslI CAYNNNNRTG 2 cut(s) 750, 1072
Msp20I TGGCCA 1 cut(s) 1078
MspA1I CMGCKG 2 cut(s) 833, 932
MspI CCGG 3 cut(s) 157, 585, 780
MspR9I CCNGG 6 cut(s) 354, 384, 545, 693, 781, 790
MunI CAATTG 3 cut(s) 55, 176, 939
Mva1269I GAATGC 2 cut(s) 130, 832
MvaI CCWGG 5 cut(s) 354, 384, 545, 693, 790
MwoI GCNNNNNNNGC 4 cut(s) 268, 277, 289, 494
NciI CCSGG 1 cut(s) 781
NdeII GATC 4 cut(s) 616, 732, 805, 991
NlaIII CATG 4 cut(s) 560, 865, 911, 973
NlaIV GGNNCC 9 cut(s) 146, 389, 390, 530, 542, 618, 734, 952, 1114
NmuCI GTSAC 1 cut(s) 817
OliI CACNNNNGTG 1 cut(s) 1072
PasI CCCWGGG 1 cut(s) 383
PctI GAATGC 2 cut(s) 130, 832
PfeI GAWTC 4 cut(s) 212, 218, 224, 842
PfoI TCCNGGA 1 cut(s) 788
PinAI ACCGGT 1 cut(s) 156
PkrI GCNGC 7 cut(s) 183, 261, 282, 294, 706, 721, 832
PleI GAGTC 1 cut(s) 868
PpsI GAGTC 1 cut(s) 868
PpuMI RGGWCCY 2 cut(s) 388, 540
Psp5II RGGWCCY 2 cut(s) 388, 540
Psp6I CCWGG 5 cut(s) 352, 382, 543, 691, 788
PspFI CCCAGC 1 cut(s) 524
PspGI CCWGG 5 cut(s) 352, 382, 543, 691, 788
PspN4I GGNNCC 9 cut(s) 146, 389, 390, 530, 542, 618, 734, 952, 1114
PspPI GGNCC 5 cut(s) 350, 380, 388, 540, 951
PspPPI RGGWCCY 2 cut(s) 388, 540
PstNI CAGNNNCTG 1 cut(s) 292
PsuI RGATCY 4 cut(s) 616, 732, 805, 991
PvuII CAGCTG 2 cut(s) 833, 932
RsaI GTAC 2 cut(s) 146, 530
RsaNI GTAC 2 cut(s) 145, 529
RseI CAYNNNNRTG 2 cut(s) 750, 1072
SalI GTCGAC 1 cut(s) 871
SaqAI TTAA 3 cut(s) 62, 605, 740
SatI GCNGC 7 cut(s) 182, 260, 281, 293, 705, 720, 831
Sau3AI GATC 4 cut(s) 616, 732, 805, 991
Sau96I GGNCC 5 cut(s) 350, 380, 388, 540, 951
SchI GAGTC 1 cut(s) 868
ScrFI CCNGG 6 cut(s) 354, 384, 545, 693, 781, 790
SduI GDGCHC 2 cut(s) 377, 786
SfaNI GCATC 1 cut(s) 484
SinI GGWCC 3 cut(s) 388, 540, 951
SmiMI CAYNNNNRTG 2 cut(s) 750, 1072
Sse9I AATT 7 cut(s) 55, 176, 560, 672, 939, 1005, 1084
SspMI CTAG 1 cut(s) 498
StyD4I CCNGG 6 cut(s) 352, 382, 543, 691, 779, 788
StyI CCWWGG 1 cut(s) 849
TaaI ACNGT 1 cut(s) 595
TaqI TCGA 2 cut(s) 845, 872
TasI AATT 7 cut(s) 55, 176, 560, 672, 939, 1005, 1084
TfiI GAWTC 4 cut(s) 212, 218, 224, 842
Tru1I TTAA 3 cut(s) 62, 605, 740
Tru9I TTAA 3 cut(s) 62, 605, 740
TscAI CASTG 3 cut(s) 382, 627, 706
TseFI GTSAC 1 cut(s) 817
TseI GCWGC 7 cut(s) 181, 259, 280, 292, 704, 719, 830
Tsp45I GTSAC 1 cut(s) 817
TspDTI ATGAA 3 cut(s) 573, 1017, 1107
TspRI CASTG 3 cut(s) 382, 627, 706
VpaK11BI GGWCC 3 cut(s) 388, 540, 951
XapI RAATTY 1 cut(s) 672
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XmiI GTMKAC 2 cut(s) 872, 913
XspI CTAG 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.