Rroxscaffold_4G00332000

Belongs to the precorrin methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002225
Physical Location & Seq
Forward (+)
65980254 .. 65984680
4427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00332000.1

Sequence Viewer

Length: 1137 bp
ATGGCTCTTGTCAGTAGGCTTCAATCTCTGTCTTCCTCTTTGTCTTCCACCCATTTCAGAAAACCCAATTCCTTAAACCCTCAACCCATTTGCTCTCTACACTTCAACAACTCCCCTTCTTACACTTCTCCTTTTACAGAGAAGCATTCCATTGAGAGGTACCAGAGAGACCAGTGGCTATACAATAACCAATTGGAGCAACCCACTCTATTCTCTCTCCCTCCTGATACTGATGCTGATGCTGATTCTGATTTCACAAGGCAGAATGACATTGCTCTGCAGCTACCAGAGCTGAGAAAGCTGCTTCAGGTGCTGCGTGAGAAGAGAGAGAGGGAAGGAGGGTGTGGTGGAGGAGGCAAGTGTGGGCCTGGCAATGTCTTCTTGGTCGGCACTGGCCCTGGGGACCCTGAACTACTCACATTGAAGGCTGTCAGAGTTGTACAGAGTGCTGATTTGTTGTTGTATGATAGGCTGGTGTCCAATCATGTGTTGGATTTTGTTGCCTCTGATGCTAGACTTGTCTATGTGGGCAAGACTGCTGGGTACCATAGCAGAACCCAGGAGGAGATACATGAACTGCTACTGAGTTTTGCTGAAGCTGGAGCTACTGTTGTGAGACTAAAAGGAGGGGATCCACTGGTGTTTGGAAGGGGTGGAGAGGAGATGGATTTTTTGCAACAGCAAGGAATTCAAGTGCAAGTTATTCCAGGTATCACCGCTGCTTCTGGGATAGCAGCAGAGCTAGGGATCCCATTAACTCACAGAGGTGTTGCAAATAGTGTAAGATTTCTGACCGGGCACTCCAGGAAGGGAGGAACCGATCCTCTTTTTGTGGCAGAGAATGCAGCTGACCCTGATTCAACCTTGGTGGTATACATGGGTTTGTCGACTCTCCCTTCTCTTGCTCAAAAGTTGGTCCATCATGGTCTGCCACCAACAACACCAGCTGTTGCAGTTGAGCGAGGGACCACACCTCAACAGCGCATGGTCTTTGCAGAACTGAAGGATCTTGCCAATGAAATTACATTAGCAGAGTTAGTATCACCAACACTCATCGTCATTGGGAAAGTAGTTGCACTTTCACCATTGTGGCCACATACTTTGAACGAAGTGGCTGCTCTTGTCGGAGCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120
Pfam Domains
Protein Families
TP_methylase

Protein Analysis

378

Amino Acids

40.82

Weight (kDa)

5.89

Isoelectric Point (pI)

34.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 126 - 338 1.4e-51 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 159, 543
AccB1I GGYRCC 2 cut(s) 159, 543
AccI GTMKAC 2 cut(s) 873, 887
AciI CCGC 1 cut(s) 717
AclWI GGATC 6 cut(s) 626, 639, 742, 755, 815, 1014
AcoI YGGCCR 1 cut(s) 1091
AcsI RAATTY 1 cut(s) 687
AcuI CTGAAG 3 cut(s) 290, 615, 1022
AfaI GTAC 3 cut(s) 161, 441, 545
AfiI CCNNNNNNNGG 1 cut(s) 156
AgsI TTSAA 6 cut(s) 23, 106, 424, 692, 861, 1105
AjnI CCWGG 5 cut(s) 367, 397, 558, 706, 803
AleI CACNNNNGTG 2 cut(s) 765, 1087
AluBI AGCT 8 cut(s) 283, 292, 301, 599, 605, 742, 848, 947
AluI AGCT 8 cut(s) 283, 292, 301, 599, 605, 742, 848, 947
Alw26I GTCTC 2 cut(s) 162, 610
AlwI GGATC 6 cut(s) 626, 639, 742, 755, 815, 1014
AlwNI CAGNNNCTG 1 cut(s) 313
AoxI GGCC 3 cut(s) 365, 394, 1091
ApeKI GCWGC 7 cut(s) 280, 301, 313, 719, 734, 845, 1115
ApoI RAATTY 1 cut(s) 687
Asp718I GGTACC 2 cut(s) 159, 543
AspLEI GCGC 1 cut(s) 984
AspS9I GGNCC 5 cut(s) 365, 395, 403, 916, 966
AsuC2I CCSGG 1 cut(s) 796
AsuHPI GGTGA 3 cut(s) 706, 1035, 1074
AvaII GGWCC 3 cut(s) 403, 916, 966
BaeGI GKGCMC 1 cut(s) 801
BalI TGGCCA 1 cut(s) 1093
BamHI GGATCC 2 cut(s) 631, 747
BanI GGYRCC 2 cut(s) 159, 543
BbsI GAAGAC 3 cut(s) 24, 36, 370
BbvI GCAGC 7 cut(s) 288, 292, 300, 706, 746, 857, 1102
BccI CCATC 2 cut(s) 658, 927
BcgI CGANNNNNNTGC 2 cut(s) 1097, 1131
BciT130I CCWGG 5 cut(s) 369, 399, 560, 708, 805
BcnI CCSGG 1 cut(s) 796
BcoDI GTCTC 2 cut(s) 162, 610
BfaI CTAG 2 cut(s) 513, 743
BfmI CTRYAG 1 cut(s) 278
BisI GCNGC 7 cut(s) 281, 302, 314, 720, 735, 846, 1116
BlsI GCNGC 7 cut(s) 282, 303, 315, 721, 736, 847, 1117
Bme1390I CCNGG 6 cut(s) 369, 399, 560, 708, 796, 805
Bme18I GGWCC 3 cut(s) 403, 916, 966
BmgT120I GGNCC 5 cut(s) 365, 395, 403, 916, 966
BmiI GGNNCC 9 cut(s) 161, 404, 405, 545, 633, 749, 817, 967, 1129
BmrFI CCNGG 6 cut(s) 369, 399, 560, 708, 796, 805
BmsI GCATC 3 cut(s) 223, 229, 499
BpiI GAAGAC 3 cut(s) 24, 36, 370
BpmI CTGGAG 2 cut(s) 621, 787
BpuMI CCSGG 1 cut(s) 796
BsaI GGTCTC 1 cut(s) 162
BsaJI CCNNGG 4 cut(s) 397, 398, 558, 864
BsaXI ACNNNNNCTCC 2 cut(s) 594, 624
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 3 cut(s) 172, 397, 642
Bse3DI GCAATG 2 cut(s) 270, 379
BseBI CCWGG 5 cut(s) 369, 399, 560, 708, 805
BseDI CCNNGG 4 cut(s) 397, 398, 558, 864
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMI GCAATG 2 cut(s) 270, 379
BseMII CTCAG 2 cut(s) 284, 575
BseNI ACTGG 3 cut(s) 172, 397, 642
BseRI GAGGAG 3 cut(s) 366, 578, 674
BseSI GKGCMC 1 cut(s) 801
BseXI GCAGC 7 cut(s) 288, 292, 300, 706, 746, 857, 1102
BseYI CCCAGC 1 cut(s) 539
BshFI GGCC 3 cut(s) 367, 396, 1093
BshNI GGYRCC 2 cut(s) 159, 543
BsiSI CCGG 1 cut(s) 795
BslFI GGGAC 2 cut(s) 416, 979
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 2 cut(s) 162, 610
BsmFI GGGAC 2 cut(s) 416, 979
BsmI GAATGC 2 cut(s) 145, 847
BsnI GGCC 3 cut(s) 367, 396, 1093
Bso31I GGTCTC 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 801
Bsp1407I TGTACA 1 cut(s) 439
Bsp143I GATC 4 cut(s) 631, 747, 820, 1006
BspACI CCGC 1 cut(s) 717
BspANI GGCC 3 cut(s) 367, 396, 1093
BspCNI CTCAG 2 cut(s) 285, 576
BspLI GGNNCC 9 cut(s) 161, 404, 405, 545, 633, 749, 817, 967, 1129
BspMAI CTGCAG 1 cut(s) 282
BspPI GGATC 6 cut(s) 626, 639, 742, 755, 815, 1014
BspT107I GGYRCC 2 cut(s) 159, 543
BspTNI GGTCTC 1 cut(s) 162
BsrDI GCAATG 2 cut(s) 270, 379
BsrGI TGTACA 1 cut(s) 439
BsrI ACTGG 3 cut(s) 172, 397, 642
BssECI CCNNGG 4 cut(s) 397, 398, 558, 864
BssMI GATC 4 cut(s) 631, 747, 820, 1006
BssNAI GTATAC 1 cut(s) 874
BssT1I CCWWGG 1 cut(s) 864
Bst1107I GTATAC 1 cut(s) 874
Bst2UI CCWGG 5 cut(s) 369, 399, 560, 708, 805
Bst4CI ACNGT 1 cut(s) 610
Bst6I CTCTTC 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 842
BstAUI TGTACA 1 cut(s) 439
BstDEI CTNAG 2 cut(s) 293, 584
BstHHI GCGC 1 cut(s) 984
BstKTI GATC 4 cut(s) 634, 750, 823, 1009
BstMAI GTCTC 2 cut(s) 162, 610
BstMBI GATC 4 cut(s) 631, 747, 820, 1006
BstMWI GCNNNNNNNGC 5 cut(s) 289, 298, 310, 509, 842
BstNI CCWGG 5 cut(s) 369, 399, 560, 708, 805
BstSCI CCNGG 6 cut(s) 367, 397, 558, 706, 794, 803
BstSFI CTRYAG 1 cut(s) 278
BstSLI GKGCMC 1 cut(s) 801
BstV1I GCAGC 7 cut(s) 288, 292, 300, 706, 746, 857, 1102
BstV2I GAAGAC 3 cut(s) 24, 36, 370
BstX2I RGATCY 3 cut(s) 631, 747, 1006
BstYI RGATCY 3 cut(s) 631, 747, 1006
BstZ17I GTATAC 1 cut(s) 874
BsuRI GGCC 3 cut(s) 367, 396, 1093
BtsIMutI CAGTG 3 cut(s) 179, 390, 635
CaiI CAGNNNCTG 1 cut(s) 313
CfoI GCGC 1 cut(s) 984
Cfr13I GGNCC 5 cut(s) 365, 395, 403, 916, 966
Csp6I GTAC 3 cut(s) 160, 440, 544
CspCI CAANNNNNGTGG 4 cut(s) 849, 884, 1083, 1118
CviAII CATG 5 cut(s) 485, 572, 877, 923, 985
CviQI GTAC 3 cut(s) 160, 440, 544
DdeI CTNAG 2 cut(s) 293, 584
DpnI GATC 4 cut(s) 633, 749, 822, 1008
DpnII GATC 4 cut(s) 631, 747, 820, 1006
EaeI YGGCCR 1 cut(s) 1091
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
Eco130I CCWWGG 1 cut(s) 864
Eco31I GGTCTC 1 cut(s) 162
Eco47I GGWCC 3 cut(s) 403, 916, 966
Eco57I CTGAAG 3 cut(s) 290, 615, 1022
EcoO109I RGGNCCY 1 cut(s) 403
EcoRI GAATTC 1 cut(s) 687
EcoRII CCWGG 5 cut(s) 367, 397, 558, 706, 803
EcoT14I CCWWGG 1 cut(s) 864
ErhI CCWWGG 1 cut(s) 864
FaeI CATG 5 cut(s) 488, 575, 880, 926, 988
FaqI GGGAC 2 cut(s) 416, 979
FatI CATG 5 cut(s) 484, 571, 876, 922, 984
FblI GTMKAC 2 cut(s) 873, 887
Fnu4HI GCNGC 7 cut(s) 281, 302, 314, 720, 735, 846, 1116
Fsp4HI GCNGC 7 cut(s) 281, 302, 314, 720, 735, 846, 1116
FspBI CTAG 2 cut(s) 513, 743
GlaI GCGC 1 cut(s) 983
GluI GCNGC 7 cut(s) 281, 302, 314, 720, 735, 846, 1116
GsaI CCCAGC 1 cut(s) 543
GsuI CTGGAG 2 cut(s) 621, 787
HaeIII GGCC 3 cut(s) 367, 396, 1093
HapII CCGG 1 cut(s) 795
HhaI GCGC 1 cut(s) 984
Hin1II CATG 5 cut(s) 488, 575, 880, 926, 988
Hin6I GCGC 1 cut(s) 982
HinP1I GCGC 1 cut(s) 982
HincII GTYRAC 1 cut(s) 888
HindII GTYRAC 1 cut(s) 888
HinfI GANTC 3 cut(s) 245, 857, 889
HpaII CCGG 1 cut(s) 795
HphI GGTGA 3 cut(s) 706, 1035, 1074
Hpy166II GTNNAC 2 cut(s) 874, 888
Hpy188I TCNGA 6 cut(s) 59, 250, 434, 508, 792, 1127
Hpy188III TCNNGA 1 cut(s) 224
Hpy8I GTNNAC 2 cut(s) 874, 888
HpyAV CCTTC 7 cut(s) 126, 329, 418, 642, 802, 906, 997
HpyCH4III ACNGT 1 cut(s) 610
HpyCH4V TGCA 8 cut(s) 280, 676, 697, 773, 845, 953, 995, 1076
HpyF10VI GCNNNNNNNGC 5 cut(s) 289, 298, 310, 509, 842
HpyF3I CTNAG 2 cut(s) 293, 584
Hsp92II CATG 5 cut(s) 488, 575, 880, 926, 988
HspAI GCGC 1 cut(s) 982
KflI GGGWCCC 1 cut(s) 403
KpnI GGTACC 2 cut(s) 163, 547
Kzo9I GATC 4 cut(s) 631, 747, 820, 1006
LmnI GCTCC 3 cut(s) 196, 602, 1127
Lsp1109I GCAGC 7 cut(s) 288, 292, 300, 706, 746, 857, 1102
LweI GCATC 3 cut(s) 223, 229, 499
MaeI CTAG 2 cut(s) 513, 743
MalI GATC 4 cut(s) 633, 749, 822, 1008
MboI GATC 4 cut(s) 631, 747, 820, 1006
MboII GAAGA 4 cut(s) 24, 36, 334, 370
MfeI CAATTG 1 cut(s) 191
MflI RGATCY 3 cut(s) 631, 747, 1006
MhlI GDGCHC 1 cut(s) 801
MlsI TGGCCA 1 cut(s) 1093
MluCI AATT 4 cut(s) 67, 191, 687, 1020
MluNI TGGCCA 1 cut(s) 1093
MlyI GAGTC 1 cut(s) 883
MmeI TCCRAC 2 cut(s) 471, 1105
Mox20I TGGCCA 1 cut(s) 1093
MscI TGGCCA 1 cut(s) 1093
MseI TTAA 2 cut(s) 74, 755
MslI CAYNNNNRTG 2 cut(s) 765, 1087
Msp20I TGGCCA 1 cut(s) 1093
MspA1I CMGCKG 3 cut(s) 719, 848, 947
MspI CCGG 1 cut(s) 795
MspR9I CCNGG 6 cut(s) 369, 399, 560, 708, 796, 805
MunI CAATTG 1 cut(s) 191
Mva1269I GAATGC 2 cut(s) 145, 847
MvaI CCWGG 5 cut(s) 369, 399, 560, 708, 805
MwoI GCNNNNNNNGC 5 cut(s) 289, 298, 310, 509, 842
NciI CCSGG 1 cut(s) 796
NdeII GATC 4 cut(s) 631, 747, 820, 1006
NlaIII CATG 5 cut(s) 488, 575, 880, 926, 988
NlaIV GGNNCC 9 cut(s) 161, 404, 405, 545, 633, 749, 817, 967, 1129
OliI CACNNNNGTG 2 cut(s) 765, 1087
PasI CCCWGGG 1 cut(s) 398
PctI GAATGC 2 cut(s) 145, 847
PfeI GAWTC 2 cut(s) 245, 857
PfoI TCCNGGA 1 cut(s) 803
PkrI GCNGC 7 cut(s) 282, 303, 315, 721, 736, 847, 1117
PleI GAGTC 1 cut(s) 883
PpsI GAGTC 1 cut(s) 883
PpuMI RGGWCCY 1 cut(s) 403
Psp5II RGGWCCY 1 cut(s) 403
Psp6I CCWGG 5 cut(s) 367, 397, 558, 706, 803
PspFI CCCAGC 1 cut(s) 539
PspGI CCWGG 5 cut(s) 367, 397, 558, 706, 803
PspN4I GGNNCC 9 cut(s) 161, 404, 405, 545, 633, 749, 817, 967, 1129
PspPI GGNCC 5 cut(s) 365, 395, 403, 916, 966
PspPPI RGGWCCY 1 cut(s) 403
PstI CTGCAG 1 cut(s) 282
PstNI CAGNNNCTG 1 cut(s) 313
PsuI RGATCY 3 cut(s) 631, 747, 1006
PvuII CAGCTG 2 cut(s) 848, 947
RsaI GTAC 3 cut(s) 161, 441, 545
RsaNI GTAC 3 cut(s) 160, 440, 544
RseI CAYNNNNRTG 2 cut(s) 765, 1087
SalI GTCGAC 1 cut(s) 886
SaqAI TTAA 2 cut(s) 74, 755
SatI GCNGC 7 cut(s) 281, 302, 314, 720, 735, 846, 1116
Sau3AI GATC 4 cut(s) 631, 747, 820, 1006
Sau96I GGNCC 5 cut(s) 365, 395, 403, 916, 966
SchI GAGTC 1 cut(s) 883
ScrFI CCNGG 6 cut(s) 369, 399, 560, 708, 796, 805
SduI GDGCHC 1 cut(s) 801
SfaNI GCATC 3 cut(s) 223, 229, 499
SfcI CTRYAG 1 cut(s) 278
SinI GGWCC 3 cut(s) 403, 916, 966
SmiMI CAYNNNNRTG 2 cut(s) 765, 1087
Sse9I AATT 4 cut(s) 67, 191, 687, 1020
SsiI CCGC 1 cut(s) 717
SspMI CTAG 2 cut(s) 513, 743
StyD4I CCNGG 6 cut(s) 367, 397, 558, 706, 794, 803
StyI CCWWGG 1 cut(s) 864
TaaI ACNGT 1 cut(s) 610
TaqI TCGA 1 cut(s) 887
TasI AATT 4 cut(s) 67, 191, 687, 1020
TatI WGTACW 1 cut(s) 439
TfiI GAWTC 2 cut(s) 245, 857
Tru1I TTAA 2 cut(s) 74, 755
Tru9I TTAA 2 cut(s) 74, 755
TscAI CASTG 3 cut(s) 179, 397, 642
TseI GCWGC 7 cut(s) 280, 301, 313, 719, 734, 845, 1115
TspDTI ATGAA 2 cut(s) 588, 1032
TspRI CASTG 3 cut(s) 179, 397, 642
VpaK11BI GGWCC 3 cut(s) 403, 916, 966
XapI RAATTY 1 cut(s) 687
XcmI CCANNNNNNNNNTGG 1 cut(s) 487
XmiI GTMKAC 2 cut(s) 873, 887
XspI CTAG 2 cut(s) 513, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.