RchiOBHm_Chr4g0408791

Belongs to the precorrin methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002225
Physical Location & Seq
Reverse (-)
31650191 .. 31653137
2947 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37987

Sequence Viewer

Length: 480 bp
ATGAATTTTTTGCAACAGCAAGGAATTCAAGTGCAAGTTATTCCAGGTATTACTGCTGCTTCTGGGATAGCAGCAGAGCTGGGGATCCCATTAACGCACAGAGGTGTTGCAAATAGTGTAAGATTTCTGACCAGGCACTCCAGGAAGGGAGGAACAGATTCTCTTTTTGTGGCAGAGAATGCAGCTGACCCTGATTTGACCTTGGTGGTATACATGGGTTTGTCGACTCTCCCTTCTCTTGCTCAAAAGTTGGTCCATCATGGTCTGCCACTAACAACTCCAGCTGTTGCAGTTGTGCGAGGGACCACACCTCAACAACGCATGGTGTTTGCAGAACTGAAGGATCTTGCCAATGAAATTTCATCAGCAGAGTTGGTATCACCAACGCTCATTGTCATTGGGAAAGTAGTTGCACTTTCACTTTCACCATTGTGGCTCTATACTTCAAACCAAGTGTCCTCTCTTGTCCGAACCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120
Pfam Domains
Protein Families
TP_methylase

Protein Analysis

159

Amino Acids

16.89

Weight (kDa)

9.3

Isoelectric Point (pI)

26.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 3 - 117 2.2e-19 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 210, 224
AclWI GGATC 3 cut(s) 79, 92, 351
AcsI RAATTY 3 cut(s) 4, 24, 357
AcuI CTGAAG 1 cut(s) 359
AgsI TTSAA 2 cut(s) 29, 447
AjnI CCWGG 3 cut(s) 43, 131, 140
AleI CACNNNNGTG 2 cut(s) 102, 430
AluBI AGCT 3 cut(s) 79, 185, 284
AluI AGCT 3 cut(s) 79, 185, 284
AlwI GGATC 3 cut(s) 79, 92, 351
ApeKI GCWGC 3 cut(s) 56, 71, 182
ApoI RAATTY 3 cut(s) 4, 24, 357
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
Asp700I GAANNNNTTC 1 cut(s) 157
AspS9I GGNCC 2 cut(s) 253, 303
AsuHPI GGTGA 2 cut(s) 372, 417
AvaII GGWCC 2 cut(s) 253, 303
BamHI GGATCC 1 cut(s) 84
BbvI GCAGC 3 cut(s) 43, 83, 194
BccI CCATC 1 cut(s) 264
BciT130I CCWGG 3 cut(s) 45, 133, 142
BisI GCNGC 3 cut(s) 57, 72, 183
BlsI GCNGC 3 cut(s) 58, 73, 184
Bme1390I CCNGG 3 cut(s) 45, 133, 142
Bme18I GGWCC 2 cut(s) 253, 303
BmgT120I GGNCC 2 cut(s) 253, 303
BmiI GGNNCC 2 cut(s) 86, 304
BmrFI CCNGG 3 cut(s) 45, 133, 142
BpmI CTGGAG 2 cut(s) 124, 264
BsaJI CCNNGG 1 cut(s) 201
BseBI CCWGG 3 cut(s) 45, 133, 142
BseDI CCNNGG 1 cut(s) 201
BseXI GCAGC 3 cut(s) 43, 83, 194
BseYI CCCAGC 1 cut(s) 79
BslFI GGGAC 1 cut(s) 316
BsmFI GGGAC 1 cut(s) 316
BsmI GAATGC 1 cut(s) 184
Bsp143I GATC 2 cut(s) 84, 343
BspLI GGNNCC 2 cut(s) 86, 304
BspPI GGATC 3 cut(s) 79, 92, 351
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 2 cut(s) 84, 343
BssNAI GTATAC 1 cut(s) 211
BssT1I CCWWGG 1 cut(s) 201
Bst1107I GTATAC 1 cut(s) 211
Bst2UI CCWGG 3 cut(s) 45, 133, 142
BstAPI GCANNNNNTGC 1 cut(s) 179
BstKTI GATC 2 cut(s) 87, 346
BstMBI GATC 2 cut(s) 84, 343
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 3 cut(s) 45, 133, 142
BstSCI CCNGG 3 cut(s) 43, 131, 140
BstV1I GCAGC 3 cut(s) 43, 83, 194
BstX2I RGATCY 2 cut(s) 84, 343
BstYI RGATCY 2 cut(s) 84, 343
BstZ17I GTATAC 1 cut(s) 211
Cfr13I GGNCC 2 cut(s) 253, 303
CviAII CATG 3 cut(s) 214, 260, 322
CviJI RGCY 4 cut(s) 79, 185, 284, 436
CviKI_1 RGCY 4 cut(s) 79, 185, 284, 436
DpnI GATC 2 cut(s) 86, 345
DpnII GATC 2 cut(s) 84, 343
Eco130I CCWWGG 1 cut(s) 201
Eco47I GGWCC 2 cut(s) 253, 303
Eco57I CTGAAG 1 cut(s) 359
EcoRI GAATTC 1 cut(s) 24
EcoRII CCWGG 3 cut(s) 43, 131, 140
EcoT14I CCWWGG 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 3 cut(s) 217, 263, 325
FaiI YATR 7 cut(s) 211, 215, 261, 323, 441, 476, 478
FaqI GGGAC 1 cut(s) 316
FatI CATG 3 cut(s) 213, 259, 321
FblI GTMKAC 2 cut(s) 210, 224
Fnu4HI GCNGC 3 cut(s) 57, 72, 183
Fsp4HI GCNGC 3 cut(s) 57, 72, 183
GluI GCNGC 3 cut(s) 57, 72, 183
GsaI CCCAGC 1 cut(s) 83
GsuI CTGGAG 2 cut(s) 124, 264
Hin1II CATG 3 cut(s) 217, 263, 325
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HinfI GANTC 2 cut(s) 158, 226
HphI GGTGA 2 cut(s) 372, 417
Hpy166II GTNNAC 2 cut(s) 211, 225
Hpy188I TCNGA 2 cut(s) 129, 470
Hpy8I GTNNAC 2 cut(s) 211, 225
HpyAV CCTTC 3 cut(s) 139, 243, 334
HpyCH4V TGCA 7 cut(s) 13, 34, 110, 182, 290, 332, 413
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
Hsp92II CATG 3 cut(s) 217, 263, 325
Kzo9I GATC 2 cut(s) 84, 343
Lsp1109I GCAGC 3 cut(s) 43, 83, 194
MalI GATC 2 cut(s) 86, 345
MboI GATC 2 cut(s) 84, 343
MflI RGATCY 2 cut(s) 84, 343
MluCI AATT 3 cut(s) 4, 24, 357
MlyI GAGTC 1 cut(s) 220
MnlI CCTC 5 cut(s) 95, 143, 293, 321, 469
MroXI GAANNNNTTC 1 cut(s) 157
MseI TTAA 1 cut(s) 92
MslI CAYNNNNRTG 2 cut(s) 102, 430
MspA1I CMGCKG 2 cut(s) 185, 284
MspR9I CCNGG 3 cut(s) 45, 133, 142
Mva1269I GAATGC 1 cut(s) 184
MvaI CCWGG 3 cut(s) 45, 133, 142
MwoI GCNNNNNNNGC 1 cut(s) 179
NdeII GATC 2 cut(s) 84, 343
NlaIII CATG 3 cut(s) 217, 263, 325
NlaIV GGNNCC 2 cut(s) 86, 304
OliI CACNNNNGTG 2 cut(s) 102, 430
PctI GAATGC 1 cut(s) 184
PdmI GAANNNNTTC 1 cut(s) 157
PfeI GAWTC 1 cut(s) 158
PfoI TCCNGGA 1 cut(s) 140
PkrI GCNGC 3 cut(s) 58, 73, 184
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
Psp6I CCWGG 3 cut(s) 43, 131, 140
PspFI CCCAGC 1 cut(s) 79
PspGI CCWGG 3 cut(s) 43, 131, 140
PspN4I GGNNCC 2 cut(s) 86, 304
PspPI GGNCC 2 cut(s) 253, 303
PsuI RGATCY 2 cut(s) 84, 343
PvuII CAGCTG 2 cut(s) 185, 284
RseI CAYNNNNRTG 2 cut(s) 102, 430
SalI GTCGAC 1 cut(s) 223
SaqAI TTAA 1 cut(s) 92
SatI GCNGC 3 cut(s) 57, 72, 183
Sau3AI GATC 2 cut(s) 84, 343
Sau96I GGNCC 2 cut(s) 253, 303
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 3 cut(s) 45, 133, 142
SetI ASST 7 cut(s) 49, 81, 106, 187, 203, 286, 313
SinI GGWCC 2 cut(s) 253, 303
SmiMI CAYNNNNRTG 2 cut(s) 102, 430
Sse9I AATT 3 cut(s) 4, 24, 357
StyD4I CCNGG 3 cut(s) 43, 131, 140
StyI CCWWGG 1 cut(s) 201
TaqI TCGA 1 cut(s) 224
TasI AATT 3 cut(s) 4, 24, 357
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 1 cut(s) 92
Tru9I TTAA 1 cut(s) 92
TseI GCWGC 3 cut(s) 56, 71, 182
TspDTI ATGAA 3 cut(s) 17, 351, 369
VpaK11BI GGWCC 2 cut(s) 253, 303
XapI RAATTY 3 cut(s) 4, 24, 357
XmiI GTMKAC 2 cut(s) 210, 224
XmnI GAANNNNTTC 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.