Rh6DG218900

Belongs to the precorrin methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002225
Physical Location & Seq
Reverse (-)
38958517 .. 38962146
3630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG218900.1

Sequence Viewer

Length: 1119 bp
ATGGCTCTTGTGAGTAAGCTTCAATCTCTGTCTTCCTCTTTGTCTTCCACCCATTTCAGAAAACCCAACCGCTTAAACCCTGAACCCATTTGCTCTCTACACTTCAACTCCCCTTCTTACACTTCTCCTTTTACAGAGAAGCATTCAATTGAGAGGTACCAGAGAGACCGGTGGCTATACAATAACCAATTGGAGCAACCCACTCTAATCTCTCTCCCTCCTGATTCTGATTCTGATTTCACAAGGCAGAATGACATTGCTTTGCAGCTACCAGAGCTGAGAAAGCTGCTTCAGGTGCTGCGAGAGAAGAGAGAGAGGGAAGGAGGGTGTGGTGGAGGCAAGTGTGGGCCTGGCAATGTCTTCTTGGTGGGCACTGGCCCTGGGGACCCTGAACTCCTGACATTGAAGGCTGTCAGAGTTATTCAGAGTGCTGATTTATTGTTGTATGATAGGTTGGTGTCCAATGATGTGTTGGATTTTGTTGCCTCTGATGCTAGACTTGTCTATGTGGGCAAGACTGCTGGGTACCATAGCAGAACCCAGGAGGAGATACATGAATTGCTACTGAGTTTTGCTGAAGCCGGAGCTACTGTTGTGAGATTAAAAGGAGGGGATCCACTGGTGTTTGGAAGGGGTGGAGAGGAGATGGATTTTTTGCAACAGCAAGGAATTCAAGTGCAAGTTATACCTGGTATCACTGCTGCTTCTGGGATAGCAGCAGAGTTTGGGATCCCATTAACTCATAGAGGTGTTGCAAATAGCATAAGATTTCTAACCGGGCACTCCAGGAAGGGAGGAACAGATCCTCTTTTTTTGACAGAGAATGCAGCTGACCCTGATTCGACCTTGGTGGTATACATGGGCTTGTCGACTCTCCCTTCTCTTGCTCAAAAGTTGGTCCATCATGGTCTGCTACCATCAACACCAGCTGTTGCAATTGAGCGAGGGACCACACCTCAACAGCGCATGGTCTTTGCAGAACTGAAGGATCTTGCCAATGAAATTACATCAGCAGAGTTAGTATCACCAACACTCATCGTCATTGGGAAAGTAGTTGCACTTTCACCATTGTGGCCACATAATTTGAATGAAGTGACCTCTCTTGCTGGAGCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120
Pfam Domains
Protein Families
TP_methylase

Protein Analysis

372

Amino Acids

40.4

Weight (kDa)

6.08

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 120 - 332 2.7e-50 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 156, 525
AccB1I GGYRCC 2 cut(s) 156, 525
AccI GTMKAC 2 cut(s) 855, 869
AciI CCGC 1 cut(s) 70
AclWI GGATC 6 cut(s) 608, 621, 724, 737, 797, 996
AcoI YGGCCR 1 cut(s) 1073
AcsI RAATTY 1 cut(s) 669
AcuI CTGAAG 3 cut(s) 275, 597, 1004
AfaI GTAC 2 cut(s) 158, 527
AgeI ACCGGT 1 cut(s) 168
AgsI TTSAA 6 cut(s) 23, 106, 147, 406, 674, 1087
AjnI CCWGG 5 cut(s) 349, 379, 540, 688, 785
AleI CACNNNNGTG 1 cut(s) 1069
AluBI AGCT 7 cut(s) 19, 268, 277, 286, 587, 830, 929
AluI AGCT 7 cut(s) 19, 268, 277, 286, 587, 830, 929
Alw26I GTCTC 1 cut(s) 159
AlwI GGATC 6 cut(s) 608, 621, 724, 737, 797, 996
AlwNI CAGNNNCTG 1 cut(s) 298
AoxI GGCC 3 cut(s) 347, 376, 1073
ApeKI GCWGC 6 cut(s) 265, 286, 298, 701, 716, 827
ApoI RAATTY 1 cut(s) 669
AsiGI ACCGGT 1 cut(s) 168
Asp718I GGTACC 2 cut(s) 156, 525
AspLEI GCGC 1 cut(s) 966
AspS9I GGNCC 5 cut(s) 347, 377, 385, 898, 948
AsuC2I CCSGG 1 cut(s) 778
AsuHPI GGTGA 2 cut(s) 1017, 1056
AvaII GGWCC 3 cut(s) 385, 898, 948
BaeGI GKGCMC 2 cut(s) 374, 783
BalI TGGCCA 1 cut(s) 1075
BamHI GGATCC 2 cut(s) 613, 729
BanI GGYRCC 2 cut(s) 156, 525
BbsI GAAGAC 3 cut(s) 24, 36, 352
BbvI GCAGC 6 cut(s) 273, 277, 285, 688, 728, 839
BccI CCATC 3 cut(s) 640, 909, 925
BciT130I CCWGG 5 cut(s) 351, 381, 542, 690, 787
BcnI CCSGG 1 cut(s) 778
BcoDI GTCTC 1 cut(s) 159
BfaI CTAG 1 cut(s) 495
BisI GCNGC 6 cut(s) 266, 287, 299, 702, 717, 828
BlsI GCNGC 6 cut(s) 267, 288, 300, 703, 718, 829
Bme1390I CCNGG 6 cut(s) 351, 381, 542, 690, 778, 787
Bme18I GGWCC 3 cut(s) 385, 898, 948
BmgT120I GGNCC 5 cut(s) 347, 377, 385, 898, 948
BmiI GGNNCC 8 cut(s) 158, 386, 387, 527, 615, 731, 949, 1111
BmrFI CCNGG 6 cut(s) 351, 381, 542, 690, 778, 787
BmsI GCATC 1 cut(s) 481
BpiI GAAGAC 3 cut(s) 24, 36, 352
BpmI CTGGAG 1 cut(s) 769
BpuMI CCSGG 1 cut(s) 778
BsaI GGTCTC 1 cut(s) 159
BsaJI CCNNGG 4 cut(s) 379, 380, 540, 846
BsaWI WCCGGW 1 cut(s) 168
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 576, 606
Bse118I RCCGGY 1 cut(s) 168
Bse1I ACTGG 2 cut(s) 379, 624
Bse3DI GCAATG 2 cut(s) 255, 361
BseBI CCWGG 5 cut(s) 351, 381, 542, 690, 787
BseDI CCNNGG 4 cut(s) 379, 380, 540, 846
BseMI GCAATG 2 cut(s) 255, 361
BseMII CTCAG 2 cut(s) 269, 557
BseNI ACTGG 2 cut(s) 379, 624
BseRI GAGGAG 2 cut(s) 560, 656
BseSI GKGCMC 2 cut(s) 374, 783
BseXI GCAGC 6 cut(s) 273, 277, 285, 688, 728, 839
BseYI CCCAGC 1 cut(s) 521
BshFI GGCC 3 cut(s) 349, 378, 1075
BshNI GGYRCC 2 cut(s) 156, 525
BshTI ACCGGT 1 cut(s) 168
BsiSI CCGG 3 cut(s) 169, 582, 777
BslFI GGGAC 2 cut(s) 398, 961
BsmAI GTCTC 1 cut(s) 159
BsmFI GGGAC 2 cut(s) 398, 961
BsmI GAATGC 2 cut(s) 142, 829
BsnI GGCC 3 cut(s) 349, 378, 1075
Bso31I GGTCTC 1 cut(s) 159
Bsp1286I GDGCHC 2 cut(s) 374, 783
Bsp143I GATC 4 cut(s) 613, 729, 802, 988
BspACI CCGC 1 cut(s) 70
BspANI GGCC 3 cut(s) 349, 378, 1075
BspCNI CTCAG 2 cut(s) 270, 558
BspLI GGNNCC 8 cut(s) 158, 386, 387, 527, 615, 731, 949, 1111
BspPI GGATC 6 cut(s) 608, 621, 724, 737, 797, 996
BspT107I GGYRCC 2 cut(s) 156, 525
BspTNI GGTCTC 1 cut(s) 159
BsrDI GCAATG 2 cut(s) 255, 361
BsrFI RCCGGY 1 cut(s) 168
BsrI ACTGG 2 cut(s) 379, 624
BssAI RCCGGY 1 cut(s) 168
BssECI CCNNGG 4 cut(s) 379, 380, 540, 846
BssMI GATC 4 cut(s) 613, 729, 802, 988
BssNAI GTATAC 1 cut(s) 856
BssT1I CCWWGG 1 cut(s) 846
Bst1107I GTATAC 1 cut(s) 856
Bst2UI CCWGG 5 cut(s) 351, 381, 542, 690, 787
Bst4CI ACNGT 1 cut(s) 592
Bst6I CTCTTC 1 cut(s) 302
BstDEI CTNAG 2 cut(s) 278, 566
BstHHI GCGC 1 cut(s) 966
BstKTI GATC 4 cut(s) 616, 732, 805, 991
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 4 cut(s) 613, 729, 802, 988
BstMWI GCNNNNNNNGC 4 cut(s) 274, 283, 295, 491
BstNI CCWGG 5 cut(s) 351, 381, 542, 690, 787
BstSCI CCNGG 6 cut(s) 349, 379, 540, 688, 776, 785
BstSLI GKGCMC 2 cut(s) 374, 783
BstV1I GCAGC 6 cut(s) 273, 277, 285, 688, 728, 839
BstV2I GAAGAC 3 cut(s) 24, 36, 352
BstX2I RGATCY 4 cut(s) 613, 729, 802, 988
BstYI RGATCY 4 cut(s) 613, 729, 802, 988
BstZ17I GTATAC 1 cut(s) 856
BsuRI GGCC 3 cut(s) 349, 378, 1075
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 3 cut(s) 372, 617, 696
CaiI CAGNNNCTG 1 cut(s) 298
CfoI GCGC 1 cut(s) 966
Cfr10I RCCGGY 1 cut(s) 168
Cfr13I GGNCC 5 cut(s) 347, 377, 385, 898, 948
CsiI ACCWGGT 1 cut(s) 688
Csp6I GTAC 2 cut(s) 157, 526
CspAI ACCGGT 1 cut(s) 168
CspCI CAANNNNNGTGG 2 cut(s) 1065, 1100
CviAII CATG 4 cut(s) 554, 859, 905, 967
CviQI GTAC 2 cut(s) 157, 526
DdeI CTNAG 2 cut(s) 278, 566
DpnI GATC 4 cut(s) 615, 731, 804, 990
DpnII GATC 4 cut(s) 613, 729, 802, 988
EaeI YGGCCR 1 cut(s) 1073
Eam1104I CTCTTC 1 cut(s) 302
EarI CTCTTC 1 cut(s) 302
Eco130I CCWWGG 1 cut(s) 846
Eco31I GGTCTC 1 cut(s) 159
Eco47I GGWCC 3 cut(s) 385, 898, 948
Eco57I CTGAAG 3 cut(s) 275, 597, 1004
EcoO109I RGGNCCY 1 cut(s) 385
EcoRI GAATTC 1 cut(s) 669
EcoRII CCWGG 5 cut(s) 349, 379, 540, 688, 785
EcoT14I CCWWGG 1 cut(s) 846
ErhI CCWWGG 1 cut(s) 846
FaeI CATG 4 cut(s) 557, 862, 908, 970
FaqI GGGAC 2 cut(s) 398, 961
FatI CATG 4 cut(s) 553, 858, 904, 966
FblI GTMKAC 2 cut(s) 855, 869
Fnu4HI GCNGC 6 cut(s) 266, 287, 299, 702, 717, 828
Fsp4HI GCNGC 6 cut(s) 266, 287, 299, 702, 717, 828
FspBI CTAG 1 cut(s) 495
GlaI GCGC 1 cut(s) 965
GluI GCNGC 6 cut(s) 266, 287, 299, 702, 717, 828
GsaI CCCAGC 1 cut(s) 525
GsuI CTGGAG 1 cut(s) 769
HaeIII GGCC 3 cut(s) 349, 378, 1075
HapII CCGG 3 cut(s) 169, 582, 777
HhaI GCGC 1 cut(s) 966
Hin1II CATG 4 cut(s) 557, 862, 908, 970
Hin6I GCGC 1 cut(s) 964
HinP1I GCGC 1 cut(s) 964
HincII GTYRAC 1 cut(s) 870
HindII GTYRAC 1 cut(s) 870
HindIII AAGCTT 1 cut(s) 17
HinfI GANTC 4 cut(s) 224, 230, 839, 871
HpaII CCGG 3 cut(s) 169, 582, 777
HphI GGTGA 2 cut(s) 1017, 1056
Hpy166II GTNNAC 2 cut(s) 856, 870
Hpy188I TCNGA 6 cut(s) 59, 229, 235, 416, 426, 490
Hpy188III TCNNGA 2 cut(s) 221, 397
Hpy8I GTNNAC 2 cut(s) 856, 870
HpyAV CCTTC 7 cut(s) 123, 314, 400, 624, 784, 888, 979
HpyCH4III ACNGT 1 cut(s) 592
HpyCH4V TGCA 8 cut(s) 265, 658, 679, 755, 827, 935, 977, 1058
HpyF10VI GCNNNNNNNGC 4 cut(s) 274, 283, 295, 491
HpyF3I CTNAG 2 cut(s) 278, 566
Hsp92II CATG 4 cut(s) 557, 862, 908, 970
HspAI GCGC 1 cut(s) 964
KflI GGGWCCC 1 cut(s) 385
KpnI GGTACC 2 cut(s) 160, 529
Kzo9I GATC 4 cut(s) 613, 729, 802, 988
LmnI GCTCC 3 cut(s) 193, 584, 1109
Lsp1109I GCAGC 6 cut(s) 273, 277, 285, 688, 728, 839
LweI GCATC 1 cut(s) 481
MabI ACCWGGT 1 cut(s) 688
MaeI CTAG 1 cut(s) 495
MaeIII GTNAC 1 cut(s) 1093
MalI GATC 4 cut(s) 615, 731, 804, 990
MboI GATC 4 cut(s) 613, 729, 802, 988
MboII GAAGA 4 cut(s) 24, 36, 319, 352
MfeI CAATTG 3 cut(s) 147, 188, 936
MflI RGATCY 4 cut(s) 613, 729, 802, 988
MhlI GDGCHC 2 cut(s) 374, 783
MlsI TGGCCA 1 cut(s) 1075
MluCI AATT 7 cut(s) 147, 188, 557, 669, 936, 1002, 1081
MluNI TGGCCA 1 cut(s) 1075
MlyI GAGTC 1 cut(s) 865
MmeI TCCRAC 1 cut(s) 453
Mox20I TGGCCA 1 cut(s) 1075
MscI TGGCCA 1 cut(s) 1075
MseI TTAA 3 cut(s) 74, 602, 737
MslI CAYNNNNRTG 2 cut(s) 747, 1069
Msp20I TGGCCA 1 cut(s) 1075
MspA1I CMGCKG 2 cut(s) 830, 929
MspI CCGG 3 cut(s) 169, 582, 777
MspR9I CCNGG 6 cut(s) 351, 381, 542, 690, 778, 787
MunI CAATTG 3 cut(s) 147, 188, 936
Mva1269I GAATGC 2 cut(s) 142, 829
MvaI CCWGG 5 cut(s) 351, 381, 542, 690, 787
MwoI GCNNNNNNNGC 4 cut(s) 274, 283, 295, 491
NciI CCSGG 1 cut(s) 778
NdeII GATC 4 cut(s) 613, 729, 802, 988
NlaIII CATG 4 cut(s) 557, 862, 908, 970
NlaIV GGNNCC 8 cut(s) 158, 386, 387, 527, 615, 731, 949, 1111
NmuCI GTSAC 1 cut(s) 1093
OliI CACNNNNGTG 1 cut(s) 1069
PasI CCCWGGG 1 cut(s) 380
PctI GAATGC 2 cut(s) 142, 829
PfeI GAWTC 3 cut(s) 224, 230, 839
PfoI TCCNGGA 1 cut(s) 785
PinAI ACCGGT 1 cut(s) 168
PkrI GCNGC 6 cut(s) 267, 288, 300, 703, 718, 829
PleI GAGTC 1 cut(s) 865
PpsI GAGTC 1 cut(s) 865
PpuMI RGGWCCY 1 cut(s) 385
Psp5II RGGWCCY 1 cut(s) 385
Psp6I CCWGG 5 cut(s) 349, 379, 540, 688, 785
PspFI CCCAGC 1 cut(s) 521
PspGI CCWGG 5 cut(s) 349, 379, 540, 688, 785
PspN4I GGNNCC 8 cut(s) 158, 386, 387, 527, 615, 731, 949, 1111
PspPI GGNCC 5 cut(s) 347, 377, 385, 898, 948
PspPPI RGGWCCY 1 cut(s) 385
PstNI CAGNNNCTG 1 cut(s) 298
PsuI RGATCY 4 cut(s) 613, 729, 802, 988
PvuII CAGCTG 2 cut(s) 830, 929
RsaI GTAC 2 cut(s) 158, 527
RsaNI GTAC 2 cut(s) 157, 526
RseI CAYNNNNRTG 2 cut(s) 747, 1069
SalI GTCGAC 1 cut(s) 868
SaqAI TTAA 3 cut(s) 74, 602, 737
SatI GCNGC 6 cut(s) 266, 287, 299, 702, 717, 828
Sau3AI GATC 4 cut(s) 613, 729, 802, 988
Sau96I GGNCC 5 cut(s) 347, 377, 385, 898, 948
SchI GAGTC 1 cut(s) 865
ScrFI CCNGG 6 cut(s) 351, 381, 542, 690, 778, 787
SduI GDGCHC 2 cut(s) 374, 783
SexAI ACCWGGT 1 cut(s) 688
SfaNI GCATC 1 cut(s) 481
SinI GGWCC 3 cut(s) 385, 898, 948
SmiMI CAYNNNNRTG 2 cut(s) 747, 1069
Sse9I AATT 7 cut(s) 147, 188, 557, 669, 936, 1002, 1081
SsiI CCGC 1 cut(s) 70
SspMI CTAG 1 cut(s) 495
StyD4I CCNGG 6 cut(s) 349, 379, 540, 688, 776, 785
StyI CCWWGG 1 cut(s) 846
TaaI ACNGT 1 cut(s) 592
TaqI TCGA 2 cut(s) 842, 869
TasI AATT 7 cut(s) 147, 188, 557, 669, 936, 1002, 1081
TfiI GAWTC 3 cut(s) 224, 230, 839
Tru1I TTAA 3 cut(s) 74, 602, 737
Tru9I TTAA 3 cut(s) 74, 602, 737
TscAI CASTG 3 cut(s) 379, 624, 703
TseFI GTSAC 1 cut(s) 1093
TseI GCWGC 6 cut(s) 265, 286, 298, 701, 716, 827
Tsp45I GTSAC 1 cut(s) 1093
TspDTI ATGAA 3 cut(s) 570, 1014, 1104
TspRI CASTG 3 cut(s) 379, 624, 703
VpaK11BI GGWCC 3 cut(s) 385, 898, 948
XapI RAATTY 1 cut(s) 669
XcmI CCANNNNNNNNNTGG 1 cut(s) 469
XmiI GTMKAC 2 cut(s) 855, 869
XspI CTAG 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.