Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0003270

Physical Location & Seq

Genomic Coordinates Forward (+)

1561793 .. 1563870

Gene Structure 2078 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1018100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1503 bp

ATGAATGTATTAAACCTTACTTTCTCCATTCTTCCAAAAAACTTTTGCAAGACAGAAAACATGTCTGGTGCATTTGAGGTGGATAAGGATGGCAATTTGGTATTCCATCAAAATCAAAATAGTGAACCTGATGAGTTCCAACAATTTCAGAAACAATTCAATTCTTCAATGCAATGGGGAGTGGCCTTATCTGTTGTTTCTGGTTTGGTGGCGACCATTGCAATTGTAGCCATCGTATATGCCATCATTGATTGCTTGAAAAAGACCGGAAGCGCTATTCCGGCATATGCCCGGATAGCCAACGGTTACAGCAGCATCGACAAGTCTTCTAACGCAAATCATTCATCCGACGATGTAGCAATCGACTTAACACCCGAATTGCAACAAGTTCGAAGCTCACAGGTTGGGTTTCCGACGGTAAGGGGGTTTCTTAGCAACATGGCGAGGGAGAAGCCGGTAGCCTTTTCACCCAAGCAACTTGCAGAATACACAGATAACTACTCCACCATATTGGGTTCAGGTGGCTTTGGAGTTGTCTTCAAAGGGATTCTCCCACATGGAGTGCAAGTCGCTGTCAAGGTGCTTACTAACACTAGGGATAAGATAGTTGAAGATCAATTCATGGCGGAAGTTGGCACCTTGGGAAGAACTTACCACATGAATTTGGTAAAACTATACGGCTTCTGCTTTGACCACGAAATGCGGGCACTTGTCTATGAGTACATGGAGAATGGATCACTTGACAAGTTCTTGTTTAGTGAGCATAGAGAAATAGGTTTGGAGAAGCTGCACGATATCGCAGTCCAAACAGCAAAAGGGTTGGCTTACCTGCATGAAGAGTGCGAACAAAGAATCATTCACTATGATATCAAGCCTGGGAATGTGCTTCTCGATAAGAATTTGACTCCAAAGGTTGCGGATTTTGGACTTGCCAAGCTTTGCAACAGAGGAAGCAGTGAAATGTTGTTAACCAAGGGAAGAGGGACACCGGGATATGCAGCCCCAGAGATGTGGAAGCCCTTTCCGGTGACTCAAAAGTGCGATGTTTACAGTTTTGGTATGCTTCTCTTTGAGATTGTTGGAAGGAGGAGAAATTTTGATGAGGATGTAAACGAGTCACGCGAATGGCTTCCAAGGTGGATCTGGAACATGTTTGACAACAATAAATTAGAAGAGGCGCTGTCATTTTGTGGGATTGAAGAACAAGACAGAGAAAAAGCAGAGAGGATTACAATGGTAGCTCTGCTGTGCATTCAGCATTCACCAGAAGCAAGGCCTCTCATGAGCAATGTAGTGCAGATGTTGGAGGGAGATAAGGAAATTGCTTCACCGCCTTCTCCTTTCCAGCATTTGCAGTCACCGCAAGGAAATTTAACACAAGATAGTGGAACAGATGAAGACTTGAGTTCATCTGCATCAGGCACAAAGACTTCTGAACAATCCGAGCGCCATTCTAATACAGCTGTGCAGAACAGATGTGAAATTGAGCTAGCTGCTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

501

Amino Acids

55.85

Weight (kDa)

5.3

Isoelectric Point (pI)

43.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	169 - 435	3.4e-49	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	169 - 432	4e-46	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003270)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g22030 FvH4_4g22030
malus_domestica	MD02G1307500.v1.1 MD07G1018100.v1.1
prunus_persica	Prupe.3G089700_v2.0.a1 Prupe.3G089700_v2.0.a1
pyrus_communis	pycom07g01390
rosa_chinensis	RchiOBHm_Chr1g0326751 RchiOBHm_Chr1g0326841 RchiOBHm_Chr1g0326851 RchiOBHm_Chr1g0326921
rosa_laevigata	RLG00000030051 RLG00000030053 RLG00000030059
rosa_multiflora	Rmu_sc0000842.1_g000010 Rmu_sc0000842.1_g000033 Rmu_sc0000842.1_g000035 Rmu_sc0003315.1_g000010 Rmu_sc0003315.1_g000016 Rmu_sc0003315.1_g000020 Rmu_sc0005098.1_g000011 Rmu_sc0017564.1_g000001 Rmu_sc0017869.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323520 Rroxscaffold_4G00323540 Rroxscaffold_4G00323560
rosa_rugosa	Rorug01G0061700 Rorug01G0062000
rosa_samantha	Rh1AG078300 Rh1AG079000 Rh1AG079200 Rh1AG079300 Rh1CG076900 Rh1DG083300
rosa_wichuraiana	Rw1G006260 Rw1G006270 Rw1G006290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	837
AccB1I	GGYRCC	1 cut(s)	635
AccII	CGCG	1 cut(s)	1122
AciI	CCGC	5 cut(s)	626, 703, 917, 1331, 1361
AclWI	GGATC	2 cut(s)	742, 1148
AcsI	RAATTY	4 cut(s)	661, 898, 1093, 1369
AfaI	GTAC	1 cut(s)	722
AfeI	AGCGCT	1 cut(s)	274
AflIII	ACRYGT	2 cut(s)	60, 1149
AgsI	TTSAA	6 cut(s)	160, 168, 259, 541, 611, 1199
AjnI	CCWGG	1 cut(s)	874
AluBI	AGCT	7 cut(s)	396, 787, 937, 1241, 1463, 1489, 1493
AluI	AGCT	7 cut(s)	396, 787, 937, 1241, 1463, 1489, 1493
AlwI	GGATC	2 cut(s)	742, 1148
Aor51HI	AGCGCT	1 cut(s)	274
AoxI	GGCC	2 cut(s)	183, 1274
ApeKI	GCWGC	4 cut(s)	312, 787, 998, 1493
ApoI	RAATTY	4 cut(s)	661, 898, 1093, 1369
Asp700I	GAANNNNTTC	2 cut(s)	155, 1128
AspLEI	GCGC	3 cut(s)	275, 1180, 1449
AsuC2I	CCSGG	2 cut(s)	292, 990
AsuHPI	GGTGA	5 cut(s)	459, 1039, 1254, 1320, 1350
AsuII	TTCGAA	1 cut(s)	391
AsuNHI	GCTAGC	1 cut(s)	1489
BaeGI	GKGCMC	1 cut(s)	709
BanI	GGYRCC	1 cut(s)	635
BbsI	GAAGAC	3 cut(s)	318, 529, 1404
BbvI	GCAGC	4 cut(s)	324, 774, 1010, 1480
BccI	CCATC	4 cut(s)	83, 114, 239, 251
BceAI	ACGGC	1 cut(s)	694
BcgI	CGANNNNNNTGC	2 cut(s)	371, 405
BciT130I	CCWGG	1 cut(s)	876
BcnI	CCSGG	2 cut(s)	292, 990
BfaI	CTAG	2 cut(s)	594, 1490
BfoI	RGCGCY	3 cut(s)	276, 1181, 1450
BfuAI	ACCTGC	1 cut(s)	837
BisI	GCNGC	4 cut(s)	313, 788, 999, 1494
BlsI	GCNGC	4 cut(s)	314, 789, 1000, 1495
Bme1390I	CCNGG	3 cut(s)	292, 876, 990
BmiI	GGNNCC	1 cut(s)	637
BmrFI	CCNGG	3 cut(s)	292, 876, 990
BmsI	GCATC	2 cut(s)	324, 1424
BmtI	GCTAGC	1 cut(s)	1493
BpiI	GAAGAC	3 cut(s)	318, 529, 1404
Bpu14I	TTCGAA	1 cut(s)	391
BpuEI	CTTGAG	1 cut(s)	1423
BpuMI	CCSGG	2 cut(s)	292, 990
BsaJI	CCNNGG	4 cut(s)	639, 875, 972, 1133
BsaWI	WCCGGW	2 cut(s)	266, 1024
BsaXI	ACNNNNNCTCC	2 cut(s)	552, 582
Bse118I	RCCGGY	1 cut(s)	454
Bse3DI	GCAATG	3 cut(s)	179, 216, 1294
BseBI	CCWGG	1 cut(s)	876
BseDI	CCNNGG	4 cut(s)	639, 875, 972, 1133
BseGI	GGATG	3 cut(s)	94, 344, 1111
BseMI	GCAATG	3 cut(s)	179, 216, 1294
BseRI	GAGGAG	1 cut(s)	1102
BseSI	GKGCMC	1 cut(s)	709
BseXI	GCAGC	4 cut(s)	324, 774, 1010, 1480
BsgI	GTGCAG	3 cut(s)	773, 1316, 1487
Bsh1236I	CGCG	1 cut(s)	1122
BshFI	GGCC	2 cut(s)	185, 1276
BshNI	GGYRCC	1 cut(s)	635
BsiSI	CCGG	6 cut(s)	267, 281, 292, 455, 989, 1025
BslFI	GGGAC	1 cut(s)	997
BsmFI	GGGAC	1 cut(s)	997
BsmI	GAATGC	2 cut(s)	1251, 1258
BsnI	GGCC	2 cut(s)	185, 1276
Bsp119I	TTCGAA	1 cut(s)	391
Bsp1286I	GDGCHC	1 cut(s)	709
Bsp143I	GATC	3 cut(s)	613, 734, 1140
BspACI	CCGC	5 cut(s)	626, 703, 917, 1331, 1361
BspANI	GGCC	2 cut(s)	185, 1276
BspFNI	CGCG	1 cut(s)	1122
BspHI	TCATGA	1 cut(s)	1281
BspLI	GGNNCC	1 cut(s)	637
BspMI	ACCTGC	1 cut(s)	837
BspOI	GCTAGC	1 cut(s)	1493
BspPI	GGATC	2 cut(s)	742, 1148
BspT104I	TTCGAA	1 cut(s)	391
BspT107I	GGYRCC	1 cut(s)	635
BsrDI	GCAATG	3 cut(s)	179, 216, 1294
BsrFI	RCCGGY	1 cut(s)	454
BssAI	RCCGGY	1 cut(s)	454
BssECI	CCNNGG	4 cut(s)	639, 875, 972, 1133
BssMI	GATC	3 cut(s)	613, 734, 1140
BssT1I	CCWWGG	3 cut(s)	639, 972, 1133
Bst2UI	CCWGG	1 cut(s)	876
Bst4CI	ACNGT	3 cut(s)	305, 418, 1052
Bst6I	CTCTTC	3 cut(s)	831, 973, 1167
BstBI	TTCGAA	1 cut(s)	391
BstC8I	GCNNGC	2 cut(s)	705, 1491
BstDEI	CTNAG	1 cut(s)	431
BstF5I	GGATG	3 cut(s)	94, 344, 1111
BstFNI	CGCG	1 cut(s)	1122
BstH2I	RGCGCY	3 cut(s)	276, 1181, 1450
BstHHI	GCGC	3 cut(s)	275, 1180, 1449
BstKTI	GATC	3 cut(s)	616, 737, 1143
BstMBI	GATC	3 cut(s)	613, 734, 1140
BstMWI	GCNNNNNNNGC	5 cut(s)	218, 227, 281, 296, 1360
BstNI	CCWGG	1 cut(s)	876
BstNSI	RCATGY	2 cut(s)	64, 1153
BstSCI	CCNGG	3 cut(s)	290, 874, 988
BstSLI	GKGCMC	1 cut(s)	709
BstUI	CGCG	1 cut(s)	1122
BstV1I	GCAGC	4 cut(s)	324, 774, 1010, 1480
BstV2I	GAAGAC	3 cut(s)	318, 529, 1404
BstX2I	RGATCY	1 cut(s)	1140
BstXI	CCANNNNNNTGG	2 cut(s)	511, 1011
BstYI	RGATCY	1 cut(s)	1140
BsuRI	GGCC	2 cut(s)	185, 1276
BtgZI	GCGATG	1 cut(s)	1056
BtsCI	GGATG	3 cut(s)	94, 344, 1111
BtsI	GCAGTG	1 cut(s)	961
BtsIMutI	CAGTG	1 cut(s)	961
BveI	ACCTGC	1 cut(s)	837
Cac8I	GCNNGC	2 cut(s)	705, 1491
CciI	TCATGA	1 cut(s)	1281
CfoI	GCGC	3 cut(s)	275, 1180, 1449
Cfr10I	RCCGGY	1 cut(s)	454
Csp6I	GTAC	1 cut(s)	721
CviAII	CATG	9 cut(s)	61, 439, 557, 622, 658, 724, 833, 1150, 1282
CviQI	GTAC	1 cut(s)	721
DdeI	CTNAG	1 cut(s)	431
DpnI	GATC	3 cut(s)	615, 736, 1142
DpnII	GATC	3 cut(s)	613, 734, 1140
Eam1104I	CTCTTC	3 cut(s)	831, 973, 1167
EarI	CTCTTC	3 cut(s)	831, 973, 1167
EciI	GGCGGA	1 cut(s)	641
Eco130I	CCWWGG	3 cut(s)	639, 972, 1133
Eco147I	AGGCCT	1 cut(s)	1276
Eco32I	GATATC	2 cut(s)	796, 868
Eco47III	AGCGCT	1 cut(s)	274
EcoRII	CCWGG	1 cut(s)	874
EcoRV	GATATC	2 cut(s)	796, 868
EcoT14I	CCWWGG	3 cut(s)	639, 972, 1133
ErhI	CCWWGG	3 cut(s)	639, 972, 1133
FaeI	CATG	9 cut(s)	64, 442, 560, 625, 661, 727, 836, 1153, 1285
FaqI	GGGAC	1 cut(s)	997
FatI	CATG	9 cut(s)	60, 438, 556, 621, 657, 723, 832, 1149, 1281
FauI	CCCGC	1 cut(s)	696
FauNDI	CATATG	1 cut(s)	286
Fnu4HI	GCNGC	4 cut(s)	313, 788, 999, 1494
FokI	GGATG	3 cut(s)	101, 331, 1118
Fsp4HI	GCNGC	4 cut(s)	313, 788, 999, 1494
FspBI	CTAG	2 cut(s)	594, 1490
GlaI	GCGC	3 cut(s)	274, 1179, 1448
GluI	GCNGC	4 cut(s)	313, 788, 999, 1494
HaeII	RGCGCY	3 cut(s)	276, 1181, 1450
HaeIII	GGCC	2 cut(s)	185, 1276
HapII	CCGG	6 cut(s)	267, 281, 292, 455, 989, 1025
HhaI	GCGC	3 cut(s)	275, 1180, 1449
Hin1II	CATG	9 cut(s)	64, 442, 560, 625, 661, 727, 836, 1153, 1285
Hin6I	GCGC	3 cut(s)	273, 1178, 1447
HinP1I	GCGC	3 cut(s)	273, 1178, 1447
HincII	GTYRAC	1 cut(s)	969
HindII	GTYRAC	1 cut(s)	969
HindIII	AAGCTT	1 cut(s)	935
HinfI	GANTC	5 cut(s)	547, 852, 904, 1030, 1115
HpaI	GTTAAC	1 cut(s)	969
HpaII	CCGG	6 cut(s)	267, 281, 292, 455, 989, 1025
HphI	GGTGA	5 cut(s)	459, 1039, 1254, 1320, 1350
Hpy166II	GTNNAC	4 cut(s)	125, 969, 1048, 1111
Hpy188I	TCNGA	5 cut(s)	150, 349, 414, 1435, 1444
Hpy188III	TCNNGA	3 cut(s)	890, 1144, 1282
Hpy8I	GTNNAC	4 cut(s)	125, 969, 1048, 1111
Hpy99I	CGWCG	2 cut(s)	353, 418
HpyAV	CCTTC	2 cut(s)	1077, 1344
HpyCH4III	ACNGT	3 cut(s)	305, 418, 1052
HpyF10VI	GCNNNNNNNGC	5 cut(s)	218, 227, 281, 296, 1360
HpyF3I	CTNAG	1 cut(s)	431
Hsp92II	CATG	9 cut(s)	64, 442, 560, 625, 661, 727, 836, 1153, 1285
HspAI	GCGC	3 cut(s)	273, 1178, 1447
KspAI	GTTAAC	1 cut(s)	969
Kzo9I	GATC	3 cut(s)	613, 734, 1140
Lsp1109I	GCAGC	4 cut(s)	324, 774, 1010, 1480
LweI	GCATC	2 cut(s)	324, 1424
MaeI	CTAG	2 cut(s)	594, 1490
MaeIII	GTNAC	4 cut(s)	305, 1027, 1116, 1356
MalI	GATC	3 cut(s)	615, 736, 1142
MboI	GATC	3 cut(s)	613, 734, 1140
MfeI	CAATTG	1 cut(s)	222
MflI	RGATCY	1 cut(s)	1140
MhlI	GDGCHC	1 cut(s)	709
MlyI	GAGTC	3 cut(s)	898, 1024, 1124
MmeI	TCCRAC	5 cut(s)	163, 372, 437, 1060, 1284
MroXI	GAANNNNTTC	2 cut(s)	155, 1128
MseI	TTAA	5 cut(s)	11, 368, 968, 1373, 1501
MslI	CAYNNNNRTG	1 cut(s)	1123
MspA1I	CMGCKG	1 cut(s)	1463
MspI	CCGG	6 cut(s)	267, 281, 292, 455, 989, 1025
MspR9I	CCNGG	3 cut(s)	292, 876, 990
MunI	CAATTG	1 cut(s)	222
Mva1269I	GAATGC	2 cut(s)	1251, 1258
MvaI	CCWGG	1 cut(s)	876
MvnI	CGCG	1 cut(s)	1122
MwoI	GCNNNNNNNGC	5 cut(s)	218, 227, 281, 296, 1360
NciI	CCSGG	2 cut(s)	292, 990
NdeI	CATATG	1 cut(s)	286
NdeII	GATC	3 cut(s)	613, 734, 1140
NheI	GCTAGC	1 cut(s)	1489
NlaIII	CATG	9 cut(s)	64, 442, 560, 625, 661, 727, 836, 1153, 1285
NlaIV	GGNNCC	1 cut(s)	637
NmuCI	GTSAC	3 cut(s)	1027, 1116, 1356
NspI	RCATGY	2 cut(s)	64, 1153
NspV	TTCGAA	1 cut(s)	391
PagI	TCATGA	1 cut(s)	1281
PceI	AGGCCT	1 cut(s)	1276
PciI	ACATGT	2 cut(s)	60, 1149
PcsI	WCGNNNNNNNCGW	1 cut(s)	1119
PctI	GAATGC	2 cut(s)	1251, 1258
PdmI	GAANNNNTTC	2 cut(s)	155, 1128
PfeI	GAWTC	2 cut(s)	547, 852
PkrI	GCNGC	4 cut(s)	314, 789, 1000, 1495
PleI	GAGTC	3 cut(s)	898, 1024, 1123
PpsI	GAGTC	3 cut(s)	898, 1024, 1123
PscI	ACATGT	2 cut(s)	60, 1149
Psp6I	CCWGG	1 cut(s)	874
PspGI	CCWGG	1 cut(s)	874
PspN4I	GGNNCC	1 cut(s)	637
PsuI	RGATCY	1 cut(s)	1140
PvuII	CAGCTG	1 cut(s)	1463
RsaI	GTAC	1 cut(s)	722
RsaNI	GTAC	1 cut(s)	721
RseI	CAYNNNNRTG	1 cut(s)	1123
SaqAI	TTAA	5 cut(s)	11, 368, 968, 1373, 1501
SatI	GCNGC	4 cut(s)	313, 788, 999, 1494
Sau3AI	GATC	3 cut(s)	613, 734, 1140
SchI	GAGTC	3 cut(s)	898, 1024, 1124
ScrFI	CCNGG	3 cut(s)	292, 876, 990
SduI	GDGCHC	1 cut(s)	709
SfaNI	GCATC	2 cut(s)	324, 1424
SfuI	TTCGAA	1 cut(s)	391
SmiMI	CAYNNNNRTG	1 cut(s)	1123
SmlI	CTYRAG	1 cut(s)	1402
SmoI	CTYRAG	1 cut(s)	1402
SseBI	AGGCCT	1 cut(s)	1276
SsiI	CCGC	5 cut(s)	626, 703, 917, 1331, 1361
SspMI	CTAG	2 cut(s)	594, 1490
StuI	AGGCCT	1 cut(s)	1276
StyD4I	CCNGG	3 cut(s)	290, 874, 988
StyI	CCWWGG	3 cut(s)	639, 972, 1133
TaaI	ACNGT	3 cut(s)	305, 418, 1052
TaqI	TCGA	4 cut(s)	318, 363, 391, 891
TatI	WGTACW	1 cut(s)	720
TfiI	GAWTC	2 cut(s)	547, 852
Tru1I	TTAA	5 cut(s)	11, 368, 968, 1373, 1501
Tru9I	TTAA	5 cut(s)	11, 368, 968, 1373, 1501
TscAI	CASTG	1 cut(s)	961
TseFI	GTSAC	3 cut(s)	1027, 1116, 1356
TseI	GCWGC	4 cut(s)	312, 787, 998, 1493
Tsp45I	GTSAC	3 cut(s)	1027, 1116, 1356
TspDTI	ATGAA	7 cut(s)	17, 333, 610, 674, 849, 1398, 1410
TspRI	CASTG	1 cut(s)	961
XapI	RAATTY	4 cut(s)	661, 898, 1093, 1369
XceI	RCATGY	2 cut(s)	64, 1153
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1140
XmnI	GAANNNNTTC	2 cut(s)	155, 1128
XspI	CTAG	2 cut(s)	594, 1490

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.