Rh1AG078300

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003270
Physical Location & Seq
Reverse (-)
13108464 .. 13109000
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG078300.1

Sequence Viewer

Length: 537 bp
ATGCTCCTAACCAATTTCAGAGGGACAGCCGGATATGCAGCCCCAGAGATGTGGATGCCTTATCCTGGGACTCACAAATGTGATGTCTACAGTAAATGTGATGTCTACAGTTTTGGTATTCTTCTCTTTGAGATTGTTGGAAGAAGAAGACACTGCGACGATAATTTGAGCCAGTCCCGAGACTGGCTACCTAAGTGGACATGGAACATGTTTGAGAAAAATGACTTAGCAGCACTATTATTGCGCTGTGGGATCGAAGAGAGAGACAGGGGAAAAGCAGAGAGGATGTTAATGATAGCTCTGTGGTGCATTCAGCATTCACCAGAAGCAAGACCTCTCATGAGCAATGTAGTGCAAATGTTGGAGGGGCATAAAGAAATTACTCCACCTCCTTTCCCCTTTGAACATTTGCAATCTGCACAACTGAATTTCAGTCAAAATAGTGGAACAGATGACGACACTAGTACTTTGGCATCAGCCACAATGATTGATCCACACCCTAGTGTGGCACATGAAATTGAGCTAGTTGTCGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

178

Amino Acids

20.35

Weight (kDa)

5.33

Isoelectric Point (pI)

58.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 13 - 121 3e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 87, 105
AclWI GGATC 2 cut(s) 260, 485
AcsI RAATTY 1 cut(s) 427
AfaI GTAC 1 cut(s) 466
AfiI CCNNNNNNNGG 3 cut(s) 65, 183, 505
AflIII ACRYGT 1 cut(s) 207
AgsI TTSAA 1 cut(s) 404
AhlI ACTAGT 1 cut(s) 461
AjnI CCWGG 1 cut(s) 64
AleI CACNNNNGTG 2 cut(s) 78, 501
AluBI AGCT 2 cut(s) 299, 523
AluI AGCT 2 cut(s) 299, 523
Alw26I GTCTC 2 cut(s) 174, 258
AlwI GGATC 2 cut(s) 260, 485
Ama87I CYCGRG 1 cut(s) 177
ApeKI GCWGC 2 cut(s) 38, 230
ApoI RAATTY 1 cut(s) 427
ArsI GACNNNNNNTTYG 2 cut(s) 69, 101
AspLEI GCGC 1 cut(s) 246
AsuHPI GGTGA 1 cut(s) 312
AvaI CYCGRG 1 cut(s) 177
BbsI GAAGAC 1 cut(s) 154
BbvI GCAGC 2 cut(s) 50, 242
BciT130I CCWGG 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 174, 258
BcuI ACTAGT 1 cut(s) 461
BfaI CTAG 3 cut(s) 462, 501, 524
BfmI CTRYAG 2 cut(s) 88, 106
BisI GCNGC 2 cut(s) 39, 231
BlsI GCNGC 2 cut(s) 40, 232
BmcAI AGTACT 1 cut(s) 466
Bme1390I CCNGG 1 cut(s) 66
BmeT110I CYCGRG 1 cut(s) 177
BmrFI CCNGG 1 cut(s) 66
BmsI GCATC 2 cut(s) 45, 482
BpiI GAAGAC 1 cut(s) 154
BsaJI CCNNGG 1 cut(s) 65
BsaXI ACNNNNNCTCC 2 cut(s) 373, 403
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 183, 505
Bse1I ACTGG 2 cut(s) 172, 188
Bse3DI GCAATG 1 cut(s) 352
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 65
BseGI GGATG 2 cut(s) 60, 291
BseLI CCNNNNNNNGG 3 cut(s) 65, 183, 505
BseMI GCAATG 1 cut(s) 352
BseNI ACTGG 2 cut(s) 172, 188
BseXI GCAGC 2 cut(s) 50, 242
BsgI GTGCAG 1 cut(s) 402
BsiHKCI CYCGRG 1 cut(s) 177
BsiSI CCGG 1 cut(s) 30
BslFI GGGAC 3 cut(s) 37, 82, 160
BslI CCNNNNNNNGG 3 cut(s) 65, 183, 505
BsmAI GTCTC 2 cut(s) 174, 258
BsmFI GGGAC 3 cut(s) 37, 82, 160
BsmI GAATGC 2 cut(s) 309, 316
BsoBI CYCGRG 1 cut(s) 177
Bsp143I GATC 2 cut(s) 252, 490
BspHI TCATGA 1 cut(s) 339
BspPI GGATC 2 cut(s) 260, 485
BsrDI GCAATG 1 cut(s) 352
BsrI ACTGG 2 cut(s) 172, 188
BssECI CCNNGG 1 cut(s) 65
BssMI GATC 2 cut(s) 252, 490
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 2 cut(s) 92, 110
Bst6I CTCTTC 1 cut(s) 252
BstDEI CTNAG 2 cut(s) 192, 226
BstF5I GGATG 2 cut(s) 60, 291
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 2 cut(s) 255, 493
BstMAI GTCTC 2 cut(s) 174, 258
BstMBI GATC 2 cut(s) 252, 490
BstMWI GCNNNNNNNGC 1 cut(s) 35
BstNI CCWGG 1 cut(s) 66
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 1 cut(s) 64
BstSFI CTRYAG 2 cut(s) 88, 106
BstV1I GCAGC 2 cut(s) 50, 242
BstV2I GAAGAC 1 cut(s) 154
BstXI CCANNNNNNTGG 1 cut(s) 51
BtsCI GGATG 2 cut(s) 60, 291
BtsI GCAGTG 1 cut(s) 151
BtsIMutI CAGTG 1 cut(s) 151
CciI TCATGA 1 cut(s) 339
CfoI GCGC 1 cut(s) 246
Csp6I GTAC 1 cut(s) 465
CspCI CAANNNNNGTGG 2 cut(s) 469, 504
CviAII CATG 4 cut(s) 201, 208, 340, 512
CviJI RGCY 7 cut(s) 29, 41, 171, 187, 299, 479, 523
CviKI_1 RGCY 7 cut(s) 29, 41, 171, 187, 299, 479, 523
CviQI GTAC 1 cut(s) 465
DdeI CTNAG 2 cut(s) 192, 226
DpnI GATC 2 cut(s) 254, 492
DpnII GATC 2 cut(s) 252, 490
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco88I CYCGRG 1 cut(s) 177
EcoRII CCWGG 1 cut(s) 64
FaeI CATG 4 cut(s) 204, 211, 343, 515
FaiI YATR 6 cut(s) 36, 202, 209, 341, 372, 513
FaqI GGGAC 3 cut(s) 37, 82, 160
FatI CATG 4 cut(s) 200, 207, 339, 511
FblI GTMKAC 2 cut(s) 87, 105
Fnu4HI GCNGC 2 cut(s) 39, 231
FokI GGATG 2 cut(s) 67, 298
Fsp4HI GCNGC 2 cut(s) 39, 231
FspBI CTAG 3 cut(s) 462, 501, 524
GlaI GCGC 1 cut(s) 245
GluI GCNGC 2 cut(s) 39, 231
HapII CCGG 1 cut(s) 30
HhaI GCGC 1 cut(s) 246
Hin1II CATG 4 cut(s) 204, 211, 343, 515
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HinfI GANTC 1 cut(s) 70
HpaII CCGG 1 cut(s) 30
HphI GGTGA 1 cut(s) 312
Hpy166II GTNNAC 3 cut(s) 88, 106, 198
Hpy188I TCNGA 1 cut(s) 20
Hpy188III TCNNGA 2 cut(s) 177, 340
Hpy8I GTNNAC 3 cut(s) 88, 106, 198
Hpy99I CGWCG 1 cut(s) 161
HpyCH4III ACNGT 2 cut(s) 92, 110
HpyCH4V TGCA 5 cut(s) 38, 309, 355, 412, 419
HpyF10VI GCNNNNNNNGC 1 cut(s) 35
HpyF3I CTNAG 2 cut(s) 192, 226
Hsp92II CATG 4 cut(s) 204, 211, 343, 515
HspAI GCGC 1 cut(s) 244
Kzo9I GATC 2 cut(s) 252, 490
LmnI GCTCC 1 cut(s) 9
LpnPI CCDG 8 cut(s) 43, 51, 57, 78, 169, 185, 253, 336
Lsp1109I GCAGC 2 cut(s) 50, 242
LweI GCATC 2 cut(s) 45, 482
MaeI CTAG 3 cut(s) 462, 501, 524
MalI GATC 2 cut(s) 254, 492
MboI GATC 2 cut(s) 252, 490
MboII GAAGA 5 cut(s) 113, 153, 156, 159, 269
MluCI AATT 5 cut(s) 13, 163, 378, 427, 516
MlyI GAGTC 1 cut(s) 64
MmeI TCCRAC 2 cut(s) 118, 342
MnlI CCTC 5 cut(s) 14, 276, 345, 358, 399
MseI TTAA 1 cut(s) 290
MslI CAYNNNNRTG 2 cut(s) 78, 501
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 1 cut(s) 66
Mva1269I GAATGC 2 cut(s) 309, 316
MvaI CCWGG 1 cut(s) 66
MwoI GCNNNNNNNGC 1 cut(s) 35
NdeII GATC 2 cut(s) 252, 490
NlaIII CATG 4 cut(s) 204, 211, 343, 515
NspI RCATGY 1 cut(s) 211
OliI CACNNNNGTG 2 cut(s) 78, 501
PagI TCATGA 1 cut(s) 339
PciI ACATGT 1 cut(s) 207
PctI GAATGC 2 cut(s) 309, 316
PkrI GCNGC 2 cut(s) 40, 232
PleI GAGTC 1 cut(s) 64
PpsI GAGTC 1 cut(s) 64
PscI ACATGT 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 64
PspGI CCWGG 1 cut(s) 64
RsaI GTAC 1 cut(s) 466
RsaNI GTAC 1 cut(s) 465
RseI CAYNNNNRTG 2 cut(s) 78, 501
SaqAI TTAA 1 cut(s) 290
SatI GCNGC 2 cut(s) 39, 231
Sau3AI GATC 2 cut(s) 252, 490
ScaI AGTACT 1 cut(s) 466
SchI GAGTC 1 cut(s) 64
ScrFI CCNGG 1 cut(s) 66
SetI ASST 5 cut(s) 193, 301, 337, 391, 525
SfaNI GCATC 2 cut(s) 45, 482
SfcI CTRYAG 2 cut(s) 88, 106
SmiMI CAYNNNNRTG 2 cut(s) 78, 501
SpeI ACTAGT 1 cut(s) 461
Sse9I AATT 5 cut(s) 13, 163, 378, 427, 516
SspMI CTAG 3 cut(s) 462, 501, 524
StyD4I CCNGG 1 cut(s) 64
TaaI ACNGT 2 cut(s) 92, 110
TaqI TCGA 2 cut(s) 255, 531
TasI AATT 5 cut(s) 13, 163, 378, 427, 516
TatI WGTACW 1 cut(s) 464
Tru1I TTAA 1 cut(s) 290
Tru9I TTAA 1 cut(s) 290
TscAI CASTG 1 cut(s) 158
TseI GCWGC 2 cut(s) 38, 230
TspDTI ATGAA 1 cut(s) 528
TspRI CASTG 1 cut(s) 158
XapI RAATTY 1 cut(s) 427
XceI RCATGY 1 cut(s) 211
XmiI GTMKAC 2 cut(s) 87, 105
XspI CTAG 3 cut(s) 462, 501, 524
ZrmI AGTACT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.