RchiOBHm_Chr1g0326851

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003270
Physical Location & Seq
Reverse (-)
15403015 .. 15404199
1185 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55642

Sequence Viewer

Length: 951 bp
ATGAGTGGTAAAAATACTGACAACGATTCCAACAAGAAAGTAGCAGTGAACTTGAGACGTGATCAATTTCCTGTAGCTTTTGATTCCCTGGCTGGGTTTTCAACAATCAAAGGGTTCCTAAGCAACATGGCAAGAGAGAAGCCTATAGCTTACTCAGCAAAGCAAATTGCTGAATTCACACATAACCATAGCTGCTACAACATTTTAGGTTTAGGTGGTTTTGGAGCAGTATATAAAGGGCTTCTTCAAAATGGAGTACAAGGGGCTGTCAAGGTCCTTACGAACAACAATAGGGCTAAGATAGTTGAAGAGCAGTTTATGGCAGAAGTTGGAGCCTTGGGAAGATCTTACCATGTCAATTTGGTTAGACTCTATGGCTTTTTCTTCGCCCCCGAAATGAAAGCCCTCGTCTACGAGTACATGGAGAATGGTTCTCTTGACAAGTTATTGTTCAATGACAATGGAGAAGTAGATTTAGAGAAGCTTCATGTTATTGCAGTCCAAACTGCAAAAGGTTTTGGTATTCTTCTCTTTGAGATTGTTGGAAGAAGAAGACACTACGATGATAATGTGAGCGAGTCCCGGGACTGGCTACCTAAGTGGACATGGAACATGTTTGAGAAAAATAACTTAGCAGCACTATTATTGTGCTGTGGGATCGAGGAGAGAGACAGGGGAAAAGCTGAGAGGATGTTACTGATAGCTTTGTGGTGCATTCAGCATTCACCAGAAGCGAGACCTCTCATGAGCAATTTAGTGCAAATGTTGGAGGGGCGTAAAGAAATTACTCCACCTCCTTGCCCCTTTGAACATTTGCAATCTTCGCAACGAAATTTCAGTCAAAATAGTGGAACAGATGACGACAATAGTACTTGTTCATCAGGCACACTTACTGATCCACGCCCTTTTTTAAGTGCTGCACATGAAATTGAGCTAGTTATCGTGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

316

Amino Acids

35.5

Weight (kDa)

6.1

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 67 - 174 1.1e-16 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 67 - 173 3.2e-13 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 411
AclWI GGATC 2 cut(s) 665, 890
AcsI RAATTY 2 cut(s) 173, 832
AfaI GTAC 3 cut(s) 258, 419, 871
AfiI CCNNNNNNNGG 2 cut(s) 93, 588
AflIII ACRYGT 1 cut(s) 612
AgsI TTSAA 5 cut(s) 102, 248, 308, 454, 809
AjiI CACGTC 1 cut(s) 59
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 7 cut(s) 77, 149, 192, 484, 683, 704, 934
AluI AGCT 7 cut(s) 77, 149, 192, 484, 683, 704, 934
Alw26I GTCTC 3 cut(s) 49, 663, 730
AlwI GGATC 2 cut(s) 665, 890
Ama87I CYCGRG 1 cut(s) 582
ApeKI GCWGC 3 cut(s) 192, 635, 917
ApoI RAATTY 2 cut(s) 173, 832
ArsI GACNNNNNNTTYG 2 cut(s) 823, 855
AspS9I GGNCC 1 cut(s) 274
AsuC2I CCSGG 2 cut(s) 583, 584
AsuHPI GGTGA 1 cut(s) 717
AvaI CYCGRG 1 cut(s) 582
AvaII GGWCC 1 cut(s) 274
BbsI GAAGAC 1 cut(s) 559
BbvI GCAGC 3 cut(s) 179, 647, 904
BciT130I CCWGG 1 cut(s) 89
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 2 cut(s) 583, 584
BcoDI GTCTC 3 cut(s) 49, 663, 730
BfaI CTAG 1 cut(s) 935
BfmI CTRYAG 2 cut(s) 72, 144
BglII AGATCT 1 cut(s) 344
BisI GCNGC 3 cut(s) 193, 636, 918
BlsI GCNGC 3 cut(s) 194, 637, 919
BmcAI AGTACT 1 cut(s) 871
Bme1390I CCNGG 3 cut(s) 89, 583, 584
Bme18I GGWCC 1 cut(s) 274
BmeT110I CYCGRG 1 cut(s) 582
BmgBI CACGTC 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 274
BmiI GGNNCC 2 cut(s) 116, 334
BmrFI CCNGG 3 cut(s) 89, 583, 584
BpiI GAAGAC 1 cut(s) 559
Bpu10I CCTNAGC 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 73
BpuMI CCSGG 2 cut(s) 583, 584
BsaI GGTCTC 1 cut(s) 730
BsaJI CCNNGG 3 cut(s) 87, 336, 582
BsaXI ACNNNNNCTCC 2 cut(s) 778, 808
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 588
Bse1I ACTGG 1 cut(s) 593
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 3 cut(s) 87, 336, 582
BseGI GGATG 1 cut(s) 696
BseLI CCNNNNNNNGG 2 cut(s) 93, 588
BseMII CTCAG 2 cut(s) 168, 675
BseNI ACTGG 1 cut(s) 593
BseRI GAGGAG 1 cut(s) 677
BseXI GCAGC 3 cut(s) 179, 647, 904
BseYI CCCAGC 1 cut(s) 92
BsgI GTGCAG 1 cut(s) 903
BsiHKCI CYCGRG 1 cut(s) 582
BsiSI CCGG 1 cut(s) 583
BslFI GGGAC 2 cut(s) 565, 599
BslI CCNNNNNNNGG 2 cut(s) 93, 588
BsmAI GTCTC 3 cut(s) 49, 663, 730
BsmBI CGTCTC 1 cut(s) 49
BsmFI GGGAC 2 cut(s) 565, 599
BsmI GAATGC 2 cut(s) 714, 721
Bso31I GGTCTC 1 cut(s) 730
BsoBI CYCGRG 1 cut(s) 582
Bsp143I GATC 4 cut(s) 61, 344, 657, 895
BspCNI CTCAG 2 cut(s) 167, 676
BspHI TCATGA 1 cut(s) 744
BspLI GGNNCC 2 cut(s) 116, 334
BspPI GGATC 2 cut(s) 665, 890
BspQI GCTCTTC 1 cut(s) 303
BspTNI GGTCTC 1 cut(s) 730
BsrI ACTGG 1 cut(s) 593
BssECI CCNNGG 3 cut(s) 87, 336, 582
BssMI GATC 4 cut(s) 61, 344, 657, 895
BssT1I CCWWGG 1 cut(s) 336
Bst2UI CCWGG 1 cut(s) 89
Bst6I CTCTTC 1 cut(s) 303
BstDEI CTNAG 6 cut(s) 119, 154, 297, 597, 631, 684
BstF5I GGATG 1 cut(s) 696
BstKTI GATC 4 cut(s) 64, 347, 660, 898
BstMAI GTCTC 3 cut(s) 49, 663, 730
BstMBI GATC 4 cut(s) 61, 344, 657, 895
BstMWI GCNNNNNNNGC 2 cut(s) 155, 823
BstNI CCWGG 1 cut(s) 89
BstNSI RCATGY 1 cut(s) 616
BstSCI CCNGG 3 cut(s) 87, 581, 582
BstSFI CTRYAG 2 cut(s) 72, 144
BstV1I GCAGC 3 cut(s) 179, 647, 904
BstV2I GAAGAC 1 cut(s) 559
BstX2I RGATCY 1 cut(s) 344
BstYI RGATCY 1 cut(s) 344
BtrI CACGTC 1 cut(s) 59
BtsCI GGATG 1 cut(s) 696
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 1 cut(s) 51
CciI TCATGA 1 cut(s) 744
Cfr13I GGNCC 1 cut(s) 274
Cfr9I CCCGGG 1 cut(s) 582
Csp6I GTAC 3 cut(s) 257, 418, 870
CviAII CATG 8 cut(s) 127, 353, 421, 488, 606, 613, 745, 923
CviQI GTAC 3 cut(s) 257, 418, 870
DdeI CTNAG 6 cut(s) 119, 154, 297, 597, 631, 684
DpnI GATC 4 cut(s) 63, 346, 659, 897
DpnII GATC 4 cut(s) 61, 344, 657, 895
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Eco130I CCWWGG 1 cut(s) 336
Eco31I GGTCTC 1 cut(s) 730
Eco47I GGWCC 1 cut(s) 274
Eco88I CYCGRG 1 cut(s) 582
EcoO109I RGGNCCY 1 cut(s) 274
EcoRI GAATTC 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 336
Esp3I CGTCTC 1 cut(s) 49
FaeI CATG 8 cut(s) 130, 356, 424, 491, 609, 616, 748, 926
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FaqI GGGAC 2 cut(s) 565, 599
FatI CATG 8 cut(s) 126, 352, 420, 487, 605, 612, 744, 922
FbaI TGATCA 1 cut(s) 61
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 3 cut(s) 193, 636, 918
FokI GGATG 1 cut(s) 703
Fsp4HI GCNGC 3 cut(s) 193, 636, 918
FspBI CTAG 1 cut(s) 935
GluI GCNGC 3 cut(s) 193, 636, 918
GsaI CCCAGC 1 cut(s) 96
HapII CCGG 1 cut(s) 583
Hin1II CATG 8 cut(s) 130, 356, 424, 491, 609, 616, 748, 926
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 4 cut(s) 26, 83, 369, 578
HpaII CCGG 1 cut(s) 583
HphI GGTGA 1 cut(s) 717
Hpy166II GTNNAC 3 cut(s) 49, 412, 603
Hpy188III TCNNGA 2 cut(s) 437, 745
Hpy8I GTNNAC 3 cut(s) 49, 412, 603
HpyCH4IV ACGT 1 cut(s) 58
HpyCH4V TGCA 6 cut(s) 497, 509, 714, 760, 817, 920
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 823
HpyF3I CTNAG 6 cut(s) 119, 154, 297, 597, 631, 684
HpySE526I ACGT 1 cut(s) 58
Hsp92II CATG 8 cut(s) 130, 356, 424, 491, 609, 616, 748, 926
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 4 cut(s) 61, 344, 657, 895
LguI GCTCTTC 1 cut(s) 303
LmnI GCTCC 2 cut(s) 224, 332
LpnPI CCDG 9 cut(s) 74, 78, 84, 101, 574, 596, 658, 741, 867
Lsp1109I GCAGC 3 cut(s) 179, 647, 904
MaeI CTAG 1 cut(s) 935
MaeII ACGT 1 cut(s) 58
MaeIII GTNAC 1 cut(s) 693
MalI GATC 4 cut(s) 63, 346, 659, 897
MboI GATC 4 cut(s) 61, 344, 657, 895
MboII GAAGA 9 cut(s) 236, 320, 354, 376, 518, 558, 561, 564, 813
MflI RGATCY 1 cut(s) 344
MluCI AATT 8 cut(s) 65, 165, 173, 358, 751, 783, 832, 927
MlyI GAGTC 2 cut(s) 363, 587
MmeI TCCRAC 4 cut(s) 54, 310, 523, 747
MnlI CCTC 6 cut(s) 416, 655, 681, 750, 763, 804
MseI TTAA 1 cut(s) 911
MslI CAYNNNNRTG 1 cut(s) 561
MspI CCGG 1 cut(s) 583
MspR9I CCNGG 3 cut(s) 89, 583, 584
Mva1269I GAATGC 2 cut(s) 714, 721
MvaI CCWGG 1 cut(s) 89
MwoI GCNNNNNNNGC 2 cut(s) 155, 823
NciI CCSGG 2 cut(s) 583, 584
NdeII GATC 4 cut(s) 61, 344, 657, 895
NlaIII CATG 8 cut(s) 130, 356, 424, 491, 609, 616, 748, 926
NlaIV GGNNCC 2 cut(s) 116, 334
NspI RCATGY 1 cut(s) 616
PagI TCATGA 1 cut(s) 744
PciI ACATGT 1 cut(s) 612
PciSI GCTCTTC 1 cut(s) 303
PctI GAATGC 2 cut(s) 714, 721
PfeI GAWTC 2 cut(s) 26, 83
PkrI GCNGC 3 cut(s) 194, 637, 919
PleI GAGTC 2 cut(s) 363, 586
PpsI GAGTC 2 cut(s) 363, 586
PpuMI RGGWCCY 1 cut(s) 274
PscI ACATGT 1 cut(s) 612
Psp5II RGGWCCY 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 87
PspFI CCCAGC 1 cut(s) 92
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 116, 334
PspPI GGNCC 1 cut(s) 274
PspPPI RGGWCCY 1 cut(s) 274
PsuI RGATCY 1 cut(s) 344
RsaI GTAC 3 cut(s) 258, 419, 871
RsaNI GTAC 3 cut(s) 257, 418, 870
RseI CAYNNNNRTG 1 cut(s) 561
SapI GCTCTTC 1 cut(s) 303
SaqAI TTAA 1 cut(s) 911
SatI GCNGC 3 cut(s) 193, 636, 918
Sau3AI GATC 4 cut(s) 61, 344, 657, 895
Sau96I GGNCC 1 cut(s) 274
ScaI AGTACT 1 cut(s) 871
SchI GAGTC 2 cut(s) 363, 587
ScrFI CCNGG 3 cut(s) 89, 583, 584
SfcI CTRYAG 2 cut(s) 72, 144
SinI GGWCC 1 cut(s) 274
SmaI CCCGGG 1 cut(s) 584
SmiMI CAYNNNNRTG 1 cut(s) 561
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 8 cut(s) 65, 165, 173, 358, 751, 783, 832, 927
SspMI CTAG 1 cut(s) 935
StyD4I CCNGG 3 cut(s) 87, 581, 582
StyI CCWWGG 1 cut(s) 336
TaiI ACGT 1 cut(s) 61
TaqI TCGA 1 cut(s) 660
TasI AATT 8 cut(s) 65, 165, 173, 358, 751, 783, 832, 927
TatI WGTACW 3 cut(s) 256, 417, 869
TfiI GAWTC 2 cut(s) 26, 83
Tru1I TTAA 1 cut(s) 911
Tru9I TTAA 1 cut(s) 911
TscAI CASTG 1 cut(s) 51
TseI GCWGC 3 cut(s) 192, 635, 917
TspDTI ATGAA 4 cut(s) 413, 476, 867, 939
TspMI CCCGGG 1 cut(s) 582
TspRI CASTG 1 cut(s) 51
VpaK11BI GGWCC 1 cut(s) 274
XapI RAATTY 2 cut(s) 173, 832
XceI RCATGY 1 cut(s) 616
XmaI CCCGGG 1 cut(s) 582
XmiI GTMKAC 1 cut(s) 411
XspI CTAG 1 cut(s) 935
ZrmI AGTACT 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.