Rroxscaffold_4G00323520

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003270
Physical Location & Seq
Forward (+)
54294114 .. 54300908
6795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00323520.1

Sequence Viewer

Length: 885 bp
ATGACTTCTTCAATAACAGTGTCAAAGATTTCGATCAATTCCGTATTCAAAACAGCTGTTGTTGCTTCTGTTGTTTCTTCTGTGGTGATGATCATTGCTGTTTCAGCCATTGTATATGCCATTATTAACTGCTTGAAAAATTATGGAGATCCTATTGCAAACTATGCCCGGCTAACAAGTAGTAACGATACCGACAACGATTCCAACAACAAAGTAGCAGTGGATCTAAGAAGTGATCAATTTCCCGTAGCTTTTGATTCCCAGGCTGAGTTTCCAACAATCAAAGGGTTCCTAAGCAACATGGCAAAAGAGAAGCCTATAACTTACTCAGCAAAGCAAATTGCAGAATTCACACATAACTATAGCTACTCTAACATATTAGGTTCAGGTGGTTTTGGAGTAGTATATAAAGGGCTTCTCGAAAATGGAGTACAAGTGGCTGTCAAGGTCCTTACGAACAACAATAGGGCTAAGATAGTAGACGAGCAGTTTATGGCAGAAGTTGGAGCCTTGGGAAGAACCTACCACGTCAATTTGGTTAGACACTATGGCTTCTGCTTCTCCCCTGAAATGAAAGCCCTCGTCTATGAGTACATGGAGAATGGTTCCCTTGACAAGTTATTGTTCAGTGAAAATGGAGAAGTAGATTCAGAGAAGCTTCATGTTATTGCAGTCCAAACTGCAAAAGGGTTGGCTTATTTGCATGAGGAGTGTGAACAAAAAATCATTCACTATGATATTAAGCCCGAAAATGTGCTTCTTGATGAGAACCTTAATCCCAAGGTTGCGGATTTTGGACTCGCCAAGCTTTGCAATAGAGGGAATAGTGAAATGCTCCTAACCAATTTCAGAGGAACAGTCGGATATGCAGCCCCAGAGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

294

Amino Acids

32.33

Weight (kDa)

5.57

Isoelectric Point (pI)

27.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 121 - 294 3.7e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 124 - 293 1.2e-34 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 480
AciI CCGC 1 cut(s) 788
AclWI GGATC 2 cut(s) 143, 231
AcsI RAATTY 1 cut(s) 347
AfaI GTAC 2 cut(s) 432, 593
AgsI TTSAA 3 cut(s) 12, 49, 136
AjiI CACGTC 1 cut(s) 529
AjnI CCWGG 1 cut(s) 261
AluBI AGCT 5 cut(s) 56, 251, 366, 658, 808
AluI AGCT 5 cut(s) 56, 251, 366, 658, 808
AlwI GGATC 2 cut(s) 143, 231
ApeKI GCWGC 1 cut(s) 869
ApoI RAATTY 1 cut(s) 347
AspS9I GGNCC 1 cut(s) 448
AsuC2I CCSGG 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 97
AvaII GGWCC 1 cut(s) 448
BbvI GCAGC 1 cut(s) 881
BciT130I CCWGG 1 cut(s) 263
BclI TGATCA 2 cut(s) 90, 235
BcnI CCSGG 1 cut(s) 169
BfmI CTRYAG 1 cut(s) 361
BisI GCNGC 1 cut(s) 870
BlsI GCNGC 1 cut(s) 871
Bme1390I CCNGG 2 cut(s) 169, 263
Bme18I GGWCC 1 cut(s) 448
BmgBI CACGTC 1 cut(s) 529
BmgT120I GGNCC 1 cut(s) 448
BmiI GGNNCC 3 cut(s) 290, 508, 607
BmrFI CCNGG 2 cut(s) 169, 263
Bpu10I CCTNAGC 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 169
BsaBI GATNNNNATC 1 cut(s) 32
BsaJI CCNNGG 3 cut(s) 261, 510, 780
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bse3DI GCAATG 1 cut(s) 93
Bse8I GATNNNNATC 1 cut(s) 32
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 3 cut(s) 261, 510, 780
BseJI GATNNNNATC 1 cut(s) 32
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 2 cut(s) 258, 342
BseRI GAGGAG 1 cut(s) 722
BseXI GCAGC 1 cut(s) 881
BsiSI CCGG 1 cut(s) 169
Bsp143I GATC 5 cut(s) 33, 90, 148, 223, 235
BspACI CCGC 1 cut(s) 788
BspCNI CTCAG 2 cut(s) 259, 341
BspLI GGNNCC 3 cut(s) 290, 508, 607
BspPI GGATC 2 cut(s) 143, 231
BsrDI GCAATG 1 cut(s) 93
BssECI CCNNGG 3 cut(s) 261, 510, 780
BssMI GATC 5 cut(s) 33, 90, 148, 223, 235
BssT1I CCWWGG 2 cut(s) 510, 780
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 2 cut(s) 19, 859
BstAPI GCANNNNNTGC 1 cut(s) 164
BstDEI CTNAG 5 cut(s) 227, 267, 293, 328, 471
BstKTI GATC 5 cut(s) 36, 93, 151, 226, 238
BstMBI GATC 5 cut(s) 33, 90, 148, 223, 235
BstMWI GCNNNNNNNGC 3 cut(s) 62, 104, 164
BstNI CCWGG 1 cut(s) 263
BstSCI CCNGG 2 cut(s) 167, 261
BstSFI CTRYAG 1 cut(s) 361
BstV1I GCAGC 1 cut(s) 881
BstX2I RGATCY 2 cut(s) 148, 223
BstYI RGATCY 2 cut(s) 148, 223
BtrI CACGTC 1 cut(s) 529
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 3 cut(s) 24, 225, 634
Cfr13I GGNCC 1 cut(s) 448
Csp6I GTAC 2 cut(s) 431, 592
CviAII CATG 4 cut(s) 301, 595, 662, 704
CviQI GTAC 2 cut(s) 431, 592
DdeI CTNAG 5 cut(s) 227, 267, 293, 328, 471
DpnI GATC 5 cut(s) 35, 92, 150, 225, 237
DpnII GATC 5 cut(s) 33, 90, 148, 223, 235
Eco130I CCWWGG 2 cut(s) 510, 780
Eco47I GGWCC 1 cut(s) 448
EcoO109I RGGNCCY 1 cut(s) 448
EcoRI GAATTC 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 261
EcoT14I CCWWGG 2 cut(s) 510, 780
ErhI CCWWGG 2 cut(s) 510, 780
FaeI CATG 4 cut(s) 304, 598, 665, 707
FatI CATG 4 cut(s) 300, 594, 661, 703
FbaI TGATCA 2 cut(s) 90, 235
FblI GTMKAC 1 cut(s) 480
Fnu4HI GCNGC 1 cut(s) 870
Fsp4HI GCNGC 1 cut(s) 870
GluI GCNGC 1 cut(s) 870
HapII CCGG 1 cut(s) 169
Hin1II CATG 4 cut(s) 304, 598, 665, 707
HindIII AAGCTT 2 cut(s) 656, 806
HinfI GANTC 4 cut(s) 200, 257, 647, 798
HpaII CCGG 1 cut(s) 169
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 2 cut(s) 481, 716
Hpy188I TCNGA 3 cut(s) 652, 851, 863
Hpy188III TCNNGA 2 cut(s) 419, 761
Hpy8I GTNNAC 2 cut(s) 481, 716
HpyCH4III ACNGT 2 cut(s) 19, 859
HpyCH4IV ACGT 1 cut(s) 528
HpyCH4V TGCA 7 cut(s) 158, 344, 671, 683, 703, 813, 869
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 104, 164
HpyF3I CTNAG 5 cut(s) 227, 267, 293, 328, 471
HpySE526I ACGT 1 cut(s) 528
Hsp92II CATG 4 cut(s) 304, 598, 665, 707
Ksp22I TGATCA 2 cut(s) 90, 235
Kzo9I GATC 5 cut(s) 33, 90, 148, 223, 235
LmnI GCTCC 2 cut(s) 506, 840
LpnPI CCDG 5 cut(s) 182, 248, 275, 372, 579
Lsp1109I GCAGC 1 cut(s) 881
MaeII ACGT 1 cut(s) 528
MaeIII GTNAC 1 cut(s) 182
MalI GATC 5 cut(s) 35, 92, 150, 225, 237
MboI GATC 5 cut(s) 33, 90, 148, 223, 235
MboII GAAGA 2 cut(s) 69, 528
MflI RGATCY 2 cut(s) 148, 223
MluCI AATT 7 cut(s) 37, 139, 239, 339, 347, 532, 844
MlyI GAGTC 1 cut(s) 792
MmeI TCCRAC 4 cut(s) 228, 299, 484, 841
MnlI CCTC 4 cut(s) 590, 700, 812, 845
MseI TTAA 3 cut(s) 126, 741, 774
MspA1I CMGCKG 1 cut(s) 56
MspI CCGG 1 cut(s) 169
MspR9I CCNGG 2 cut(s) 169, 263
MvaI CCWGG 1 cut(s) 263
MwoI GCNNNNNNNGC 3 cut(s) 62, 104, 164
NciI CCSGG 1 cut(s) 169
NdeII GATC 5 cut(s) 33, 90, 148, 223, 235
NlaIII CATG 4 cut(s) 304, 598, 665, 707
NlaIV GGNNCC 3 cut(s) 290, 508, 607
PfeI GAWTC 3 cut(s) 200, 257, 647
PkrI GCNGC 1 cut(s) 871
PleI GAGTC 1 cut(s) 792
PpsI GAGTC 1 cut(s) 792
PpuMI RGGWCCY 1 cut(s) 448
Psp5II RGGWCCY 1 cut(s) 448
Psp6I CCWGG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 3 cut(s) 290, 508, 607
PspPI GGNCC 1 cut(s) 448
PspPPI RGGWCCY 1 cut(s) 448
PsuI RGATCY 2 cut(s) 148, 223
PvuII CAGCTG 1 cut(s) 56
RsaI GTAC 2 cut(s) 432, 593
RsaNI GTAC 2 cut(s) 431, 592
SaqAI TTAA 3 cut(s) 126, 741, 774
SatI GCNGC 1 cut(s) 870
Sau3AI GATC 5 cut(s) 33, 90, 148, 223, 235
Sau96I GGNCC 1 cut(s) 448
SchI GAGTC 1 cut(s) 792
ScrFI CCNGG 2 cut(s) 169, 263
SfcI CTRYAG 1 cut(s) 361
SinI GGWCC 1 cut(s) 448
Sse9I AATT 7 cut(s) 37, 139, 239, 339, 347, 532, 844
SsiI CCGC 1 cut(s) 788
StyD4I CCNGG 2 cut(s) 167, 261
StyI CCWWGG 2 cut(s) 510, 780
TaaI ACNGT 2 cut(s) 19, 859
TaiI ACGT 1 cut(s) 531
TaqI TCGA 2 cut(s) 32, 420
TasI AATT 7 cut(s) 37, 139, 239, 339, 347, 532, 844
TatI WGTACW 2 cut(s) 430, 591
TfiI GAWTC 3 cut(s) 200, 257, 647
Tru1I TTAA 3 cut(s) 126, 741, 774
Tru9I TTAA 3 cut(s) 126, 741, 774
TscAI CASTG 3 cut(s) 24, 225, 634
TseI GCWGC 1 cut(s) 869
TspDTI ATGAA 2 cut(s) 587, 650
TspGWI ACGGA 1 cut(s) 31
TspRI CASTG 3 cut(s) 24, 225, 634
VpaK11BI GGWCC 1 cut(s) 448
XapI RAATTY 1 cut(s) 347
XmiI GTMKAC 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.