MD07G1165700.v1.1

Belongs to the leguminous lectin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001977
Physical Location & Seq
Forward (+)
24037202 .. 24037753
552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1165700.v1.1.491

Sequence Viewer

Length: 552 bp
ATGGTCGCATCTTGCATCTCCACCCACTTCGTTGCATTAGATTTCCTCGTTTTCTTCCTCAGAACCACCTTCACCGCCTCCAACTCCTCATTTTCGTTCACTGATTTCGATAAAGTTTCAAACTTTGGGTCATATATTGCTCTGTACGGAGATGCAGAGGTCGTCAATGGCAGATACGCAGTTCAACTCACCAGTTCAATGAGCTCGAGCGTCGGCCGAGCTATTTACAAGAAACCCATCGAGCTGTATGAAGGTAAGCCTTGGAAATCTGTGTCTTTTTTGACGAATTTCTTGTTTTCTATTTACAACGGTGGTGGGGATGGATTGACATTTGTAATGGTTCCAAAGGGTTTTAATCTTAGCCTATTTGGGCTTTCTCTGGGAAATGGTAAAAGTAAATTCAAAGTTGCTGCGGTTAAATTTGATGCATTGAGTGATGGAAATATTCATGTTGGAATTGAAGTGGGTAGTTCTATTTCTGCTAAGAATTTGGATCCAAGTAGTGGGAATAAAACGCAGGCTTTGATTGATTACGAAGCGGGTTCGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB

Protein Analysis

184

Amino Acids

19.65

Weight (kDa)

7.79

Isoelectric Point (pI)

21.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 29 - 182 1.6e-25 Legume lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 503
AciI CCGC 3 cut(s) 75, 413, 539
AclWI GGATC 2 cut(s) 488, 501
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 4 cut(s) 286, 398, 419, 487
AfaI GTAC 1 cut(s) 146
AfiI CCNNNNNNNGG 1 cut(s) 503
AgsI TTSAA 5 cut(s) 120, 185, 198, 403, 461
AluBI AGCT 3 cut(s) 204, 221, 244
AluI AGCT 3 cut(s) 204, 221, 244
Alw21I GWGCWC 1 cut(s) 206
AlwI GGATC 2 cut(s) 488, 501
Ama87I CYCGRG 1 cut(s) 205
AoxI GGCC 1 cut(s) 214
ApeKI GCWGC 1 cut(s) 410
ApoI RAATTY 4 cut(s) 286, 398, 419, 487
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
AsuHPI GGTGA 2 cut(s) 64, 181
AsuII TTCGAA 1 cut(s) 545
AvaI CYCGRG 1 cut(s) 205
BamHI GGATCC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 206
BbvI GCAGC 1 cut(s) 397
BccI CCATC 3 cut(s) 245, 314, 431
BisI GCNGC 1 cut(s) 411
BlsI GCNGC 1 cut(s) 412
BmeT110I CYCGRG 1 cut(s) 205
BmiI GGNNCC 2 cut(s) 342, 495
BmsI GCATC 4 cut(s) 17, 24, 142, 415
Bpu14I TTCGAA 1 cut(s) 545
BsaJI CCNNGG 1 cut(s) 260
Bsc4I CCNNNNNNNGG 1 cut(s) 503
Bse1I ACTGG 1 cut(s) 192
BseDI CCNNGG 1 cut(s) 260
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 1 cut(s) 503
BseMII CTCAG 1 cut(s) 73
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 76
BseX3I CGGCCG 1 cut(s) 214
BseXI GCAGC 1 cut(s) 397
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 1 cut(s) 216
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 206
BsiHKCI CYCGRG 1 cut(s) 205
BslI CCNNNNNNNGG 1 cut(s) 503
BsnI GGCC 1 cut(s) 216
BsoBI CYCGRG 1 cut(s) 205
Bsp119I TTCGAA 1 cut(s) 545
Bsp1286I GDGCHC 1 cut(s) 206
Bsp143I GATC 1 cut(s) 493
BspACI CCGC 3 cut(s) 75, 413, 539
BspANI GGCC 1 cut(s) 216
BspCNI CTCAG 1 cut(s) 72
BspLI GGNNCC 2 cut(s) 342, 495
BspPI GGATC 2 cut(s) 488, 501
BspT104I TTCGAA 1 cut(s) 545
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 1 cut(s) 260
BssMI GATC 1 cut(s) 493
BssT1I CCWWGG 1 cut(s) 260
Bst4CI ACNGT 1 cut(s) 311
BstBI TTCGAA 1 cut(s) 545
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 3 cut(s) 59, 359, 483
BstF5I GGATG 1 cut(s) 325
BstKTI GATC 1 cut(s) 496
BstMBI GATC 1 cut(s) 493
BstMCI CGRYCG 1 cut(s) 217
BstV1I GCAGC 1 cut(s) 397
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
BstZI CGGCCG 1 cut(s) 214
BsuRI GGCC 1 cut(s) 216
BtsCI GGATG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 519
CseI GACGC 1 cut(s) 199
Csp6I GTAC 1 cut(s) 145
CspCI CAANNNNNGTGG 2 cut(s) 295, 330
CviAII CATG 1 cut(s) 449
CviJI RGCY 8 cut(s) 204, 216, 221, 244, 259, 363, 373, 521
CviKI_1 RGCY 8 cut(s) 204, 216, 221, 244, 259, 363, 373, 521
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 3 cut(s) 59, 359, 483
DpnI GATC 1 cut(s) 495
DpnII GATC 1 cut(s) 493
EaeI YGGCCR 1 cut(s) 214
EagI CGGCCG 1 cut(s) 214
Ecl136II GAGCTC 1 cut(s) 204
EclXI CGGCCG 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 260
Eco24I GRGCYC 1 cut(s) 206
Eco52I CGGCCG 1 cut(s) 214
Eco53kI GAGCTC 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 205
EcoICRI GAGCTC 1 cut(s) 204
EcoT14I CCWWGG 1 cut(s) 260
EcoT22I ATGCAT 1 cut(s) 430
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 260
FaeI CATG 1 cut(s) 452
FaiI YATR 4 cut(s) 133, 135, 249, 450
FatI CATG 1 cut(s) 448
FauI CCCGC 1 cut(s) 532
Fnu4HI GCNGC 1 cut(s) 411
FokI GGATG 1 cut(s) 332
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 1 cut(s) 411
GluI GCNGC 1 cut(s) 411
HaeIII GGCC 1 cut(s) 216
HgaI GACGC 1 cut(s) 199
Hin1II CATG 1 cut(s) 452
HphI GGTGA 2 cut(s) 64, 181
Hpy166II GTNNAC 1 cut(s) 99
Hpy188I TCNGA 1 cut(s) 62
Hpy8I GTNNAC 1 cut(s) 99
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 2 cut(s) 79, 245
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4V TGCA 4 cut(s) 15, 35, 155, 428
HpyF3I CTNAG 3 cut(s) 59, 359, 483
Hsp92II CATG 1 cut(s) 452
Kzo9I GATC 1 cut(s) 493
LpnPI CCDG 3 cut(s) 205, 365, 503
Lsp1109I GCAGC 1 cut(s) 397
LweI GCATC 4 cut(s) 17, 24, 142, 415
MalI GATC 1 cut(s) 495
MboI GATC 1 cut(s) 493
MboII GAAGA 1 cut(s) 46
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 1 cut(s) 206
MluCI AATT 6 cut(s) 286, 398, 419, 456, 487, 547
MmeI TCCRAC 2 cut(s) 105, 433
MnlI CCTC 5 cut(s) 56, 68, 88, 97, 151
Mph1103I ATGCAT 1 cut(s) 430
MseI TTAA 2 cut(s) 354, 417
NdeII GATC 1 cut(s) 493
NlaIII CATG 1 cut(s) 452
NlaIV GGNNCC 2 cut(s) 342, 495
NmeAIII GCCGAG 1 cut(s) 242
NsiI ATGCAT 1 cut(s) 430
NspV TTCGAA 1 cut(s) 545
PaeR7I CTCGAG 1 cut(s) 205
PflMI CCANNNNNTGG 1 cut(s) 503
PkrI GCNGC 1 cut(s) 412
Psp124BI GAGCTC 1 cut(s) 206
PspN4I GGNNCC 2 cut(s) 342, 495
PspXI VCTCGAGB 1 cut(s) 205
PsuI RGATCY 1 cut(s) 493
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
SacI GAGCTC 1 cut(s) 206
SaqAI TTAA 2 cut(s) 354, 417
SatI GCNGC 1 cut(s) 411
Sau3AI GATC 1 cut(s) 493
SduI GDGCHC 1 cut(s) 206
SetI ASST 6 cut(s) 71, 162, 206, 223, 246, 256
SfaNI GCATC 4 cut(s) 17, 24, 142, 415
Sfr274I CTCGAG 1 cut(s) 205
SfuI TTCGAA 1 cut(s) 545
SlaI CTCGAG 1 cut(s) 205
SmlI CTYRAG 1 cut(s) 205
SmoI CTYRAG 1 cut(s) 205
Sse9I AATT 6 cut(s) 286, 398, 419, 456, 487, 547
SsiI CCGC 3 cut(s) 75, 413, 539
SspI AATATT 1 cut(s) 445
SstI GAGCTC 1 cut(s) 206
StyI CCWWGG 1 cut(s) 260
TaaI ACNGT 1 cut(s) 311
TaqI TCGA 4 cut(s) 108, 206, 240, 545
TasI AATT 6 cut(s) 286, 398, 419, 456, 487, 547
Tru1I TTAA 2 cut(s) 354, 417
Tru9I TTAA 2 cut(s) 354, 417
TscAI CASTG 1 cut(s) 106
TseI GCWGC 1 cut(s) 410
TspDTI ATGAA 2 cut(s) 264, 437
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 1 cut(s) 106
Van91I CCANNNNNTGG 1 cut(s) 503
XapI RAATTY 4 cut(s) 286, 398, 419, 487
XhoI CTCGAG 1 cut(s) 205
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.