Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0000991

Physical Location & Seq

Genomic Coordinates Forward (+)

35014718 .. 35018576

Gene Structure 3859 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1289400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1491 bp

ATGGAGCCAGAGGCAACTATGGAAGCTACCTGCAGCACTCATGAGGAGTCAAGCACAAAAGAAGATCACCATCGTCTGATTCTAAGTCCGGCGTCACGCTTGTTCCATTCCCCGCGGTTCAACTGTTACATCGTTATTATCATGGGATTCAAGACGAGGATCGATTCAAACATTGTCAAAAATGGTTTGAAAGTCTTGCACAATCATCCTCGCTTCTCTTGCAGGCTGGATGACAGAGGCGGAAAGAAGAGATGGATTCAAGTTTCGGTAAATCAAGAAGACCATGTTTTTGTTCCAACCTTGAATTCAAAAATAGAACATCCGGAGGAATTTGTAGAAAACTACATTTCCAACCTTACTGCATCACCTCTTGACCTTTCCAAGCCGTTGTGGGAGGTACATCTCCTCAACGTCAAAACCTCGAACGCAGAAGGGCTTGCAGTGATCCGGATGCACCACTCTATGGGAGATGGCGCATCTCTCATGTCCCTTTTGCTAGCATGTACTAGAAAAACATCCGACCCAAATGCACTGCCTAGTGTTCCCACCCAGAAACGGGCGGATTCCTCAAGTGATCTGGGCTGGTTCTGGCGTTTCATCTTGGCTATTTGGTATGGTATCACACTGATAGCGAACACTTTGGTCGATATAATGTTGTTTATAGCAACACTTCTATTTTTGAACGACACAGAAACTCCTATCAAAGGTTCATCTGATGTTCATCTAACCACCAAACGCTTCTTGCATCGGACTGTCAGTATGAATCACATAAAGCAAGTGAAGAATGCGATGGGCACGACTGTCAATGATGTTGTCTTGGGAGTGACACAAGCGGGTCTCTCGTGCTATTTGAATCGCAGATACGGCCAAAATGTGAAAGATGTCGGAACAAAGCAGGAAGGGAGCAATCTTCCGAAAAATATGCACGTCAGAGCTAACATTCTCGTTAATTTAAGACCAACTGTTGGGATCCAGGATCTGGCTGATATGATGGCTAAGAAGTCCAAGGTCAGGTGGGGTAATTGTATAGGTTATATCCTTATCCCCTTCACCATTGCTCTACAAGATGACCCGTTGGATTATGTTCGCGGAGCCAAAGCTGTCATTGATCGGAAAAAACACTCCCTCGAAGCTTTCTTCACATACCTGACTGCCAATCTCTTAATCAAAATAATAGGAGCTAAGGCAGCAGGAGTTTTAGCACACAGAATTATTTCTCATTCTACATTCTCCTTCTCAAACTTGGTTGGTCCCCAGGAAGAAATTAGTTTTTACGGCCATCAAATAACTTTCATGTCTCCTCACGTTTACGGGCATCCTCACGCATTGCATATGCATTGGCAAAGCTACGCGGATGAAATGACAGTTTGCTTAACTGTTGATCCGAATGTAATACCTGATCCTGAGAAGTTGTTGGACGATTTAGAGGAATCGCTTAAACTCATTAGTCACGCTGTCATGGAAAGAGGGCTCGCCGTGGACGTTGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009987 GO:0010025 GO:0010166 GO:0012505 GO:0016020 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0019432 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0047196 GO:0071704 GO:0071944 GO:1901568 GO:1901570 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00073 map00073

Pfam Domains

Protein Families

DUF1298 WES_acyltransf

Protein Analysis

497

Amino Acids

55.66

Weight (kDa)

7.75

Isoelectric Point (pI)

41.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WS_DGAT_cat	PF03007	83 - 272	8.9e-15	Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C	PF06974	339 - 482	4.8e-51	WS/DGAT C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000991)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49190 AT3G49200 AT3G49210 AT3G49210 AT5G53390
fragaria_vesca	FvH4_5g22430 FvH4_7g31660 FvH4_7g31660 FvH4_7g31660 FvH4_7g31660 FvH4_7g31660
malus_domestica	MD07G1289400.v1.1
prunus_persica	Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1
pyrus_communis	pycom07g26220
rosa_chinensis	RchiOBHm_Chr1g0380751 RchiOBHm_Chr1g0380761 RchiOBHm_Chr1g0380771 RchiOBHm_Chr1g0380861 RchiOBHm_Chr7g0210181 RchiOBHm_Chr7g0210211
rosa_laevigata	RLG00000003058 RLG00000026267 RLG00000026272
rosa_multiflora	Rmu_sc0001637.1_g000015 Rmu_sc0002892.1_g000011 Rmu_sc0002962.1_g000013 Rmu_sc0002962.1_g000014 Rmu_sc0002962.1_g000015 Rmu_sc0005438.1_g000009 Rmu_sc0006908.1_g000008
rosa_roxburghii	Rroxscaffold_3G00248550 Rroxscaffold_3G00248560 Rroxscaffold_4G00279010
rosa_rugosa	Rorug01G0424000 Rorug01G0424400 Rorug01G0424500 Rorug07G0119100
rosa_samantha	Rh1AG448900 Rh1BG403800 Rh1BG403900 Rh1BG404400 Rh1CG418100 Rh1CG418500 Rh1DG434000 Rh1DG434100 Rh1DG434800 Rh7AG252600 Rh7BG247700 Rh7CG269600 Rh7DG260200
rosa_wichuraiana	Rw0G005940 Rw1G039120 Rw7G021480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	38
AccB7I	CCANNNNNTGG	3 cut(s)	463, 965, 979
AccII	CGCG	3 cut(s)	115, 1089, 1352
AccIII	TCCGGA	2 cut(s)	322, 447
AciI	CCGC	7 cut(s)	113, 115, 240, 560, 833, 1089, 1352
AclWI	GGATC	7 cut(s)	167, 439, 964, 977, 984, 1376, 1394
AcoI	YGGCCR	2 cut(s)	865, 1276
AcsI	RAATTY	2 cut(s)	304, 329
AcyI	GRCGYC	1 cut(s)	92
AfaI	GTAC	2 cut(s)	399, 505
AfiI	CCNNNNNNNGG	7 cut(s)	463, 555, 556, 704, 965, 979, 1011
AgsI	TTSAA	9 cut(s)	121, 151, 168, 190, 260, 304, 309, 682, 853
AjiI	CACGTC	1 cut(s)	928
AjnI	CCWGG	2 cut(s)	972, 1254
AjuI	GAANNNNNNNTTGG	2 cut(s)	289, 321
AluBI	AGCT	6 cut(s)	26, 935, 1100, 1133, 1181, 1347
AluI	AGCT	6 cut(s)	26, 935, 1100, 1133, 1181, 1347
Alw26I	GTCTC	2 cut(s)	842, 1302
AlwI	GGATC	7 cut(s)	167, 439, 964, 977, 984, 1376, 1394
AlwNI	CAGNNNCTG	1 cut(s)	979
Aor13HI	TCCGGA	2 cut(s)	322, 447
AoxI	GGCC	2 cut(s)	865, 1276
ApeKI	GCWGC	2 cut(s)	33, 1187
ApoI	RAATTY	2 cut(s)	304, 329
ArsI	GACNNNNNNTTYG	2 cut(s)	272, 304
Asp700I	GAANNNNTTC	1 cut(s)	1213
AspLEI	GCGC	1 cut(s)	476
AspS9I	GGNCC	1 cut(s)	1250
AsuHPI	GGTGA	3 cut(s)	59, 357, 1042
AsuNHI	GCTAGC	1 cut(s)	496
AvaII	GGWCC	1 cut(s)	1250
BaeGI	GKGCMC	1 cut(s)	797
BamHI	GGATCC	1 cut(s)	969
BanII	GRGCYC	1 cut(s)	1473
BauI	CACGAG	1 cut(s)	841
BbsI	GAAGAC	1 cut(s)	285
BbvI	GCAGC	2 cut(s)	45, 1199
BccI	CCATC	6 cut(s)	78, 246, 464, 784, 985, 1287
BceAI	ACGGC	4 cut(s)	370, 880, 1291, 1460
BcgI	CGANNNNNNTGC	4 cut(s)	509, 543, 904, 938
BciT130I	CCWGG	2 cut(s)	974, 1256
BcoDI	GTCTC	2 cut(s)	842, 1302
BfaI	CTAG	4 cut(s)	497, 507, 537, 1489
BfmI	CTRYAG	1 cut(s)	31
BfuAI	ACCTGC	1 cut(s)	38
BisI	GCNGC	2 cut(s)	34, 1188
BlsI	GCNGC	2 cut(s)	35, 1189
Bme1390I	CCNGG	2 cut(s)	974, 1256
Bme18I	GGWCC	1 cut(s)	1250
BmgBI	CACGTC	1 cut(s)	928
BmgT120I	GGNCC	1 cut(s)	1250
BmiI	GGNNCC	4 cut(s)	6, 971, 1093, 1252
BmrFI	CCNGG	2 cut(s)	974, 1256
BmsI	GCATC	5 cut(s)	371, 441, 485, 754, 1324
BmtI	GCTAGC	1 cut(s)	500
BpiI	GAAGAC	1 cut(s)	285
Bpu10I	CCTNAGC	1 cut(s)	1182
BpuEI	CTTGAG	1 cut(s)	553
Bsa29I	ATCGAT	1 cut(s)	162
BsaBI	GATNNNNATC	2 cut(s)	69, 720
BsaHI	GRCGYC	1 cut(s)	92
BsaI	GGTCTC	1 cut(s)	842
BsaJI	CCNNGG	4 cut(s)	113, 1005, 1254, 1476
BsaWI	WCCGGW	2 cut(s)	322, 447
BsaXI	ACNNNNNCTCC	2 cut(s)	679, 709
Bsc4I	CCNNNNNNNGG	7 cut(s)	463, 555, 556, 704, 965, 979, 1011
Bse3DI	GCAATG	2 cut(s)	1053, 1325
Bse8I	GATNNNNATC	2 cut(s)	69, 720
BseAI	TCCGGA	2 cut(s)	322, 447
BseBI	CCWGG	2 cut(s)	974, 1256
BseCI	ATCGAT	1 cut(s)	162
BseDI	CCNNGG	4 cut(s)	113, 1005, 1254, 1476
BseGI	GGATG	7 cut(s)	205, 235, 319, 456, 515, 1315, 1360
BseJI	GATNNNNATC	2 cut(s)	69, 720
BseLI	CCNNNNNNNGG	7 cut(s)	463, 555, 556, 704, 965, 979, 1011
BseMI	GCAATG	2 cut(s)	1053, 1325
BseMII	CTCAG	1 cut(s)	1395
BseRI	GAGGAG	3 cut(s)	59, 395, 1290
BseSI	GKGCMC	1 cut(s)	797
BseXI	GCAGC	2 cut(s)	45, 1199
Bsh1236I	CGCG	3 cut(s)	115, 1089, 1352
BshFI	GGCC	2 cut(s)	867, 1278
BshVI	ATCGAT	1 cut(s)	162
BsiSI	CCGG	3 cut(s)	89, 323, 448
BslFI	GGGAC	2 cut(s)	472, 1236
BslI	CCNNNNNNNGG	7 cut(s)	463, 555, 556, 704, 965, 979, 1011
BsmAI	GTCTC	2 cut(s)	842, 1302
BsmFI	GGGAC	2 cut(s)	472, 1236
BsmI	GAATGC	1 cut(s)	790
BsnI	GGCC	2 cut(s)	867, 1278
Bso31I	GGTCTC	1 cut(s)	842
Bsp1286I	GDGCHC	2 cut(s)	797, 1473
Bsp13I	TCCGGA	2 cut(s)	322, 447
Bsp143I	GATC	9 cut(s)	64, 159, 444, 574, 969, 976, 1108, 1381, 1399
BspACI	CCGC	7 cut(s)	113, 115, 240, 560, 833, 1089, 1352
BspANI	GGCC	2 cut(s)	867, 1278
BspCNI	CTCAG	1 cut(s)	1396
BspDI	ATCGAT	1 cut(s)	162
BspEI	TCCGGA	2 cut(s)	322, 447
BspFNI	CGCG	3 cut(s)	115, 1089, 1352
BspHI	TCATGA	1 cut(s)	40
BspLI	GGNNCC	4 cut(s)	6, 971, 1093, 1252
BspMAI	CTGCAG	1 cut(s)	35
BspMI	ACCTGC	1 cut(s)	38
BspOI	GCTAGC	1 cut(s)	500
BspPI	GGATC	7 cut(s)	167, 439, 964, 977, 984, 1376, 1394
BspTNI	GGTCTC	1 cut(s)	842
BsrDI	GCAATG	2 cut(s)	1053, 1325
BssECI	CCNNGG	4 cut(s)	113, 1005, 1254, 1476
BssMI	GATC	9 cut(s)	64, 159, 444, 574, 969, 976, 1108, 1381, 1399
BssNI	GRCGYC	1 cut(s)	92
BssSI	CACGAG	1 cut(s)	841
BssT1I	CCWWGG	1 cut(s)	1005
Bst2BI	CACGAG	1 cut(s)	841
Bst2UI	CCWGG	2 cut(s)	974, 1256
Bst4CI	ACNGT	6 cut(s)	125, 754, 802, 964, 1366, 1378
Bst6I	CTCTTC	1 cut(s)	242
BstACI	GRCGYC	1 cut(s)	92
BstC8I	GCNNGC	4 cut(s)	224, 438, 498, 1473
BstDEI	CTNAG	4 cut(s)	83, 996, 1182, 1404
BstDSI	CCRYGG	2 cut(s)	113, 1476
BstENI	CCTNNNNNAGG	1 cut(s)	702
BstF5I	GGATG	7 cut(s)	205, 235, 319, 456, 515, 1315, 1360
BstFNI	CGCG	3 cut(s)	115, 1089, 1352
BstHHI	GCGC	1 cut(s)	476
BstKTI	GATC	9 cut(s)	67, 162, 447, 577, 972, 979, 1111, 1384, 1402
BstMAI	GTCTC	2 cut(s)	842, 1302
BstMBI	GATC	9 cut(s)	64, 159, 444, 574, 969, 976, 1108, 1381, 1399
BstMWI	GCNNNNNNNGC	3 cut(s)	219, 864, 1187
BstNI	CCWGG	2 cut(s)	974, 1256
BstNSI	RCATGY	1 cut(s)	504
BstSCI	CCNGG	2 cut(s)	972, 1254
BstSFI	CTRYAG	1 cut(s)	31
BstSLI	GKGCMC	1 cut(s)	797
BstUI	CGCG	3 cut(s)	115, 1089, 1352
BstV1I	GCAGC	2 cut(s)	45, 1199
BstV2I	GAAGAC	1 cut(s)	285
BstX2I	RGATCY	2 cut(s)	969, 976
BstYI	RGATCY	2 cut(s)	969, 976
Bsu15I	ATCGAT	1 cut(s)	162
BsuRI	GGCC	2 cut(s)	867, 1278
BsuTUI	ATCGAT	1 cut(s)	162
BtgI	CCRYGG	2 cut(s)	113, 1476
BtgZI	GCGATG	1 cut(s)	803
BtrI	CACGTC	1 cut(s)	928
BtsCI	GGATG	7 cut(s)	205, 235, 319, 456, 515, 1315, 1360
BtsI	GCAGTG	2 cut(s)	447, 530
BtsIMutI	CAGTG	3 cut(s)	447, 530, 623
BveI	ACCTGC	1 cut(s)	38
Cac8I	GCNNGC	4 cut(s)	224, 438, 498, 1473
CaiI	CAGNNNCTG	1 cut(s)	979
CciI	TCATGA	1 cut(s)	40
CfoI	GCGC	1 cut(s)	476
Cfr13I	GGNCC	1 cut(s)	1250
Cfr42I	CCGCGG	1 cut(s)	116
ClaI	ATCGAT	1 cut(s)	162
CseI	GACGC	1 cut(s)	81
Csp6I	GTAC	2 cut(s)	398, 504
CviAII	CATG	7 cut(s)	41, 142, 284, 484, 501, 1294, 1459
CviQI	GTAC	2 cut(s)	398, 504
DdeI	CTNAG	4 cut(s)	83, 996, 1182, 1404
DpnI	GATC	9 cut(s)	66, 161, 446, 576, 971, 978, 1110, 1383, 1401
DpnII	GATC	9 cut(s)	64, 159, 444, 574, 969, 976, 1108, 1381, 1399
EaeI	YGGCCR	2 cut(s)	865, 1276
Eam1104I	CTCTTC	1 cut(s)	242
EarI	CTCTTC	1 cut(s)	242
EciI	GGCGGA	2 cut(s)	255, 575
Eco130I	CCWWGG	1 cut(s)	1005
Eco24I	GRGCYC	1 cut(s)	1473
Eco31I	GGTCTC	1 cut(s)	842
Eco47I	GGWCC	1 cut(s)	1250
EcoNI	CCTNNNNNAGG	1 cut(s)	702
EcoRI	GAATTC	1 cut(s)	304
EcoRII	CCWGG	2 cut(s)	972, 1254
EcoT14I	CCWWGG	1 cut(s)	1005
EcoT22I	ATGCAT	1 cut(s)	1338
EcoT38I	GRGCYC	1 cut(s)	1473
ErhI	CCWWGG	1 cut(s)	1005
FaeI	CATG	7 cut(s)	44, 145, 287, 487, 504, 1297, 1462
FaqI	GGGAC	2 cut(s)	472, 1236
FatI	CATG	7 cut(s)	40, 141, 283, 483, 500, 1293, 1458
FauI	CCCGC	2 cut(s)	120, 826
FauNDI	CATATG	1 cut(s)	1332
Fnu4HI	GCNGC	2 cut(s)	34, 1188
FokI	GGATG	7 cut(s)	192, 242, 306, 463, 502, 1302, 1367
FriOI	GRGCYC	1 cut(s)	1473
Fsp4HI	GCNGC	2 cut(s)	34, 1188
FspBI	CTAG	4 cut(s)	497, 507, 537, 1489
GlaI	GCGC	1 cut(s)	475
GluI	GCNGC	2 cut(s)	34, 1188
HaeIII	GGCC	2 cut(s)	867, 1278
HapII	CCGG	3 cut(s)	89, 323, 448
HgaI	GACGC	1 cut(s)	81
HhaI	GCGC	1 cut(s)	476
Hin1I	GRCGYC	1 cut(s)	92
Hin1II	CATG	7 cut(s)	44, 145, 287, 487, 504, 1297, 1462
Hin6I	GCGC	1 cut(s)	474
HinP1I	GCGC	1 cut(s)	474
HindIII	AAGCTT	1 cut(s)	1131
HinfI	GANTC	9 cut(s)	47, 79, 147, 164, 256, 563, 763, 853, 1430
HpaII	CCGG	3 cut(s)	89, 323, 448
HphI	GGTGA	3 cut(s)	59, 357, 1042
Hpy166II	GTNNAC	2 cut(s)	1309, 1480
Hpy188I	TCNGA	9 cut(s)	78, 520, 715, 750, 887, 915, 932, 1113, 1386
Hpy188III	TCNNGA	7 cut(s)	41, 151, 275, 323, 371, 448, 1403
Hpy8I	GTNNAC	2 cut(s)	1309, 1480
HpyAV	CCTTC	4 cut(s)	425, 893, 1057, 1243
HpyCH4III	ACNGT	6 cut(s)	125, 754, 802, 964, 1366, 1378
HpyCH4IV	ACGT	4 cut(s)	411, 927, 1305, 1482
HpyF10VI	GCNNNNNNNGC	3 cut(s)	219, 864, 1187
HpyF3I	CTNAG	4 cut(s)	83, 996, 1182, 1404
HpySE526I	ACGT	4 cut(s)	411, 927, 1305, 1482
Hsp92I	GRCGYC	1 cut(s)	92
Hsp92II	CATG	7 cut(s)	44, 145, 287, 487, 504, 1297, 1462
HspAI	GCGC	1 cut(s)	474
Kpn2I	TCCGGA	2 cut(s)	322, 447
KspI	CCGCGG	1 cut(s)	116
Kzo9I	GATC	9 cut(s)	64, 159, 444, 574, 969, 976, 1108, 1381, 1399
LmnI	GCTCC	4 cut(s)	4, 903, 1091, 1178
Lsp1109I	GCAGC	2 cut(s)	45, 1199
LweI	GCATC	5 cut(s)	371, 441, 485, 754, 1324
MaeI	CTAG	4 cut(s)	497, 507, 537, 1489
MaeII	ACGT	4 cut(s)	411, 927, 1305, 1482
MaeIII	GTNAC	4 cut(s)	93, 125, 823, 1448
MalI	GATC	9 cut(s)	66, 161, 446, 576, 971, 978, 1110, 1383, 1401
MboI	GATC	9 cut(s)	64, 159, 444, 574, 969, 976, 1108, 1381, 1399
MboII	GAAGA	7 cut(s)	74, 259, 290, 793, 902, 1129, 1271
MflI	RGATCY	2 cut(s)	969, 976
MhlI	GDGCHC	2 cut(s)	797, 1473
MluCI	AATT	6 cut(s)	304, 329, 949, 1021, 1209, 1263
MlyI	GAGTC	1 cut(s)	56
MmeI	TCCRAC	6 cut(s)	320, 375, 543, 865, 1056, 1395
Mph1103I	ATGCAT	1 cut(s)	1338
MroI	TCCGGA	2 cut(s)	322, 447
MroXI	GAANNNNTTC	1 cut(s)	1213
MseI	TTAA	5 cut(s)	948, 953, 1163, 1373, 1437
MspA1I	CMGCKG	1 cut(s)	115
MspI	CCGG	3 cut(s)	89, 323, 448
MspR9I	CCNGG	2 cut(s)	974, 1256
Mva1269I	GAATGC	1 cut(s)	790
MvaI	CCWGG	2 cut(s)	974, 1256
MvnI	CGCG	3 cut(s)	115, 1089, 1352
MwoI	GCNNNNNNNGC	3 cut(s)	219, 864, 1187
NdeI	CATATG	1 cut(s)	1332
NdeII	GATC	9 cut(s)	64, 159, 444, 574, 969, 976, 1108, 1381, 1399
NheI	GCTAGC	1 cut(s)	496
NlaIII	CATG	7 cut(s)	44, 145, 287, 487, 504, 1297, 1462
NlaIV	GGNNCC	4 cut(s)	6, 971, 1093, 1252
NmuCI	GTSAC	3 cut(s)	93, 823, 1448
NsiI	ATGCAT	1 cut(s)	1338
NspI	RCATGY	1 cut(s)	504
PagI	TCATGA	1 cut(s)	40
PcsI	WCGNNNNNNNCGW	1 cut(s)	1479
PctI	GAATGC	1 cut(s)	790
PdmI	GAANNNNTTC	1 cut(s)	1213
PfeI	GAWTC	8 cut(s)	79, 147, 164, 256, 563, 763, 853, 1430
PflFI	GACNNNGTC	1 cut(s)	1484
PflMI	CCANNNNNTGG	3 cut(s)	463, 965, 979
PfoI	TCCNGGA	1 cut(s)	972
PkrI	GCNGC	2 cut(s)	35, 1189
PleI	GAGTC	1 cut(s)	55
PpsI	GAGTC	1 cut(s)	55
Psp6I	CCWGG	2 cut(s)	972, 1254
PspGI	CCWGG	2 cut(s)	972, 1254
PspN4I	GGNNCC	4 cut(s)	6, 971, 1093, 1252
PspPI	GGNCC	1 cut(s)	1250
PstI	CTGCAG	1 cut(s)	35
PstNI	CAGNNNCTG	1 cut(s)	979
PsuI	RGATCY	2 cut(s)	969, 976
PsyI	GACNNNGTC	1 cut(s)	1484
RsaI	GTAC	2 cut(s)	399, 505
RsaNI	GTAC	2 cut(s)	398, 504
SacII	CCGCGG	1 cut(s)	116
SaqAI	TTAA	5 cut(s)	948, 953, 1163, 1373, 1437
SatI	GCNGC	2 cut(s)	34, 1188
Sau3AI	GATC	9 cut(s)	64, 159, 444, 574, 969, 976, 1108, 1381, 1399
Sau96I	GGNCC	1 cut(s)	1250
SchI	GAGTC	1 cut(s)	56
ScrFI	CCNGG	2 cut(s)	974, 1256
SduI	GDGCHC	2 cut(s)	797, 1473
SfaNI	GCATC	5 cut(s)	371, 441, 485, 754, 1324
SfcI	CTRYAG	1 cut(s)	31
Sfr303I	CCGCGG	1 cut(s)	116
SgrBI	CCGCGG	1 cut(s)	116
SinI	GGWCC	1 cut(s)	1250
SmlI	CTYRAG	1 cut(s)	568
SmoI	CTYRAG	1 cut(s)	568
Sse9I	AATT	6 cut(s)	304, 329, 949, 1021, 1209, 1263
SsiI	CCGC	7 cut(s)	113, 115, 240, 560, 833, 1089, 1352
SspMI	CTAG	4 cut(s)	497, 507, 537, 1489
StyD4I	CCNGG	2 cut(s)	972, 1254
StyI	CCWWGG	1 cut(s)	1005
TaaI	ACNGT	6 cut(s)	125, 754, 802, 964, 1366, 1378
TaiI	ACGT	4 cut(s)	414, 930, 1308, 1485
TaqI	TCGA	4 cut(s)	162, 422, 645, 1128
TasI	AATT	6 cut(s)	304, 329, 949, 1021, 1209, 1263
TatI	WGTACW	1 cut(s)	503
TfiI	GAWTC	8 cut(s)	79, 147, 164, 256, 563, 763, 853, 1430
Tru1I	TTAA	5 cut(s)	948, 953, 1163, 1373, 1437
Tru9I	TTAA	5 cut(s)	948, 953, 1163, 1373, 1437
TscAI	CASTG	3 cut(s)	447, 537, 630
TseFI	GTSAC	3 cut(s)	93, 823, 1448
TseI	GCWGC	2 cut(s)	33, 1187
Tsp45I	GTSAC	3 cut(s)	93, 823, 1448
TspDTI	ATGAA	6 cut(s)	586, 699, 710, 776, 1282, 1371
TspRI	CASTG	3 cut(s)	447, 537, 630
Tth111I	GACNNNGTC	1 cut(s)	1484
Van91I	CCANNNNNTGG	3 cut(s)	463, 965, 979
VpaK11BI	GGWCC	1 cut(s)	1250
XagI	CCTNNNNNAGG	1 cut(s)	702
XapI	RAATTY	2 cut(s)	304, 329
XceI	RCATGY	1 cut(s)	504
XmnI	GAANNNNTTC	1 cut(s)	1213
XspI	CTAG	4 cut(s)	497, 507, 537, 1489
Zsp2I	ATGCAT	1 cut(s)	1338

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.