Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0000991

Physical Location & Seq

Genomic Coordinates Reverse (-)

1930522 .. 1934934

Gene Structure 4413 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00279010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1515 bp

ATGGAGAACACAAGCAGCTTGCTGCTGAGCTTTGAGGCAGAGATGGAAGATCAGCAACCAAGTCTAAGCCCGGCAGCGCGTCTGTTTCACTCACCAAAGTTCAGCTGCTATATTGTCATAAGAATGGGGTGCAGCATAAGGATCAATCCTGAAGTAATCAGCGCCGGCTTGAAGGAAACCTTGTATAAGCATCCTCGCTTCTCTAGCAAAATGGTGAAGGATAGAAAATTATGTGGTAAAAAGAGATGGATACAAGTTCCAATAAATATGGAGGACCATGTTATTGTTCCAAACATGGATTCAGATATGGAGGATGCTGACCAGTTTGTTAGAGACTACATTTCCCACTTTACCAAAAGCCCTCTTGACCTTTCCAAGCCACTATGGGAAGTCCATATCCTCAACGTCAAAACCTCTGATGCAGAAGCCGTTGCAGTGATTCGGATTCACCACTCGATGGGGGATGGCACGTCCCTCATGTCACTTCTACTAGCTTGTACCAGAAAAACATTGGACCCGGATGCATTGCCTAGTGTTCCAACCAAGAGGAAACATGAGGGTAGTTCTTCAAGTGGATCGAGCGTGTTCTGGCGGTTGTTATTGGCTATTTGGTCAGCAGTAACATTGATAGGGAACACTCTTGTTGATGTTGTGCTGTTTGTAGCCACTATTCTGGTTTTGAAAGACACAAAGACTCCTATTAAAGGTCCACCTGGGGTTGAGCTTACTGCCAAGCGCTTCTTGCATCGAACGGTTAGTTTGGATCACATCAAACAAGTGAAGAATGCTATGAACATGACCATCAATGATGTTGTATTAGGAGTGACACAGGCAGGTCTATCGCGATATTTGAACTGCAGATATGCAGCAGGTGAAAATGAGAAATATGAGGGAGCAAAGAAGAAGGGACATAATCTACCAAAAAGTATCCGCCTTAGAGCAAATATTCTCGTTAATTTACGACCAGCTGTTGGGATCCAGGAACTGGCCGATATGATGGCTAAGAAGTCCAAGACCAAGTGGGGTAATAGGATTGGATATGTCCTGGTCCCCTTCACCATTGCTCTACAAGATGACCCTTTGGATTATGTTCGTGGAGCCAAGTTGCTTATGGATCGGAAAAAGCACTCATTTGAAGCATTTTGCACATACTTAAGTGCCAATCTTTTAACCAAACTAATCGGAGCAAAGGGAGCTGCAGCAGTAGCACACAGAGTTCTTTGTAATTCCACATTAGCATTCACGAACTTGATTGGTCCCCTCGAAGAAATTAGCTATTATGGGCATCCAATTACTTACATTGCTCCTAATGTTTACGGCCACCCACATGCATTACATATGCATTTTCAAAGTTACGTTGACAAAATGACTATTAGCTTAGCCTTTGACCCCGATTCAATCCCTGATCCTGAAAAGTTGTTGGATGATTTAGAAGAATCACTCAAACTCATTCGCGATGCTGTTGTCGAAAAGGAGCTCAACATTGCCAAGGAAGGAAGAAGCAGCTGTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009987 GO:0010025 GO:0010166 GO:0012505 GO:0016020 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0019432 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0047196 GO:0071704 GO:0071944 GO:1901568 GO:1901570 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00073 map00073

Pfam Domains

Protein Families

DUF1298 WES_acyltransf

Protein Analysis

504

Amino Acids

56.18

Weight (kDa)

8.94

Isoelectric Point (pI)

36.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WS_DGAT_cat	PF03007	58 - 272	8.3e-18	Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C	PF06974	341 - 484	2.5e-50	WS/DGAT C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000991)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49190 AT3G49200 AT3G49210 AT3G49210 AT5G53390
fragaria_vesca	FvH4_5g22430 FvH4_7g31660 FvH4_7g31660 FvH4_7g31660 FvH4_7g31660 FvH4_7g31660
malus_domestica	MD07G1289400.v1.1
prunus_persica	Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1 Prupe.2G310700_v2.0.a1
pyrus_communis	pycom07g26220
rosa_chinensis	RchiOBHm_Chr1g0380751 RchiOBHm_Chr1g0380761 RchiOBHm_Chr1g0380771 RchiOBHm_Chr1g0380861 RchiOBHm_Chr7g0210181 RchiOBHm_Chr7g0210211
rosa_laevigata	RLG00000003058 RLG00000026267 RLG00000026272
rosa_multiflora	Rmu_sc0001637.1_g000015 Rmu_sc0002892.1_g000011 Rmu_sc0002962.1_g000013 Rmu_sc0002962.1_g000014 Rmu_sc0002962.1_g000015 Rmu_sc0005438.1_g000009 Rmu_sc0006908.1_g000008
rosa_roxburghii	Rroxscaffold_3G00248550 Rroxscaffold_3G00248560 Rroxscaffold_4G00279010
rosa_rugosa	Rorug01G0424000 Rorug01G0424400 Rorug01G0424500 Rorug07G0119100
rosa_samantha	Rh1AG448900 Rh1BG403800 Rh1BG403900 Rh1BG404400 Rh1CG418100 Rh1CG418500 Rh1DG434000 Rh1DG434100 Rh1DG434800 Rh7AG252600 Rh7BG247700 Rh7CG269600 Rh7DG260200
rosa_wichuraiana	Rw0G005940 Rw1G039120 Rw7G021480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	860
Acc36I	ACCTGC	2 cut(s)	824, 860
AccB7I	CCANNNNNTGG	3 cut(s)	457, 971, 985
AccII	CGCG	3 cut(s)	79, 844, 1455
AciI	CCGC	2 cut(s)	592, 931
AclWI	GGATC	7 cut(s)	149, 583, 771, 970, 983, 1122, 1400
AcoI	YGGCCR	2 cut(s)	987, 1318
AcuI	CTGAAG	1 cut(s)	171
AfaI	GTAC	1 cut(s)	499
AfeI	AGCGCT	1 cut(s)	737
AfiI	CCNNNNNNNGG	4 cut(s)	457, 704, 971, 985
AflII	CTTAAG	1 cut(s)	1153
AgsI	TTSAA	7 cut(s)	172, 570, 682, 853, 1136, 1349, 1398
AjiI	CACGTC	1 cut(s)	471
AjnI	CCWGG	3 cut(s)	712, 978, 1044
AjuI	GAANNNNNNNTTGG	2 cut(s)	283, 315
Alw21I	GWGCWC	1 cut(s)	1479
Alw26I	GTCTC	1 cut(s)	327
AlwI	GGATC	7 cut(s)	149, 583, 771, 970, 983, 1122, 1400
AlwNI	CAGNNNCTG	1 cut(s)	985
Aor51HI	AGCGCT	1 cut(s)	737
AoxI	GGCC	2 cut(s)	987, 1318
ApeKI	GCWGC	9 cut(s)	15, 22, 74, 105, 132, 866, 1196, 1199, 1503
AspLEI	GCGC	3 cut(s)	79, 164, 738
AspS9I	GGNCC	5 cut(s)	274, 514, 707, 1048, 1256
AsuC2I	CCSGG	2 cut(s)	71, 518
AsuHPI	GGTGA	5 cut(s)	84, 226, 440, 884, 1048
AvaII	GGWCC	5 cut(s)	274, 514, 707, 1048, 1256
BamHI	GGATCC	1 cut(s)	975
BanII	GRGCYC	1 cut(s)	1479
Bbv12I	GWGCWC	1 cut(s)	1479
BbvI	GCAGC	8 cut(s)	9, 27, 86, 92, 144, 878, 1183, 1211
BccI	CCATC	6 cut(s)	37, 240, 451, 458, 809, 991
BceAI	ACGGC	2 cut(s)	413, 1333
BcgI	CGANNNNNNTGC	2 cut(s)	822, 856
BciT130I	CCWGG	3 cut(s)	714, 980, 1046
BciVI	GTATCC	2 cut(s)	243, 938
BcnI	CCSGG	2 cut(s)	71, 518
BcoDI	GTCTC	1 cut(s)	327
BfaI	CTAG	3 cut(s)	204, 491, 531
BfmI	CTRYAG	2 cut(s)	856, 1197
BfoI	RGCGCY	2 cut(s)	165, 739
BfrI	CTTAAG	1 cut(s)	1153
BfuAI	ACCTGC	2 cut(s)	824, 860
BfuI	GTATCC	2 cut(s)	243, 938
BisI	GCNGC	9 cut(s)	16, 23, 75, 106, 133, 867, 1197, 1200, 1504
BlpI	GCTNAGC	2 cut(s)	26, 1378
BlsI	GCNGC	9 cut(s)	17, 24, 76, 107, 134, 868, 1198, 1201, 1505
Bme1390I	CCNGG	5 cut(s)	71, 518, 714, 980, 1046
Bme18I	GGWCC	5 cut(s)	274, 514, 707, 1048, 1256
BmgBI	CACGTC	1 cut(s)	471
BmgT120I	GGNCC	5 cut(s)	274, 514, 707, 1048, 1256
BmiI	GGNNCC	5 cut(s)	516, 977, 1050, 1099, 1258
BmrFI	CCNGG	5 cut(s)	71, 518, 714, 980, 1046
BmsI	GCATC	7 cut(s)	199, 304, 409, 511, 754, 1294, 1447
Bpu1102I	GCTNAGC	2 cut(s)	26, 1378
BpuMI	CCSGG	2 cut(s)	71, 518
BsaJI	CCNNGG	2 cut(s)	713, 1488
BsaXI	ACNNNNNCTCC	2 cut(s)	679, 709
Bsc4I	CCNNNNNNNGG	4 cut(s)	457, 704, 971, 985
Bse118I	RCCGGY	1 cut(s)	164
Bse1I	ACTGG	2 cut(s)	322, 990
Bse3DI	GCAATG	4 cut(s)	524, 1059, 1299, 1482
BseBI	CCWGG	3 cut(s)	714, 980, 1046
BseDI	CCNNGG	2 cut(s)	713, 1488
BseGI	GGATG	6 cut(s)	190, 319, 469, 526, 1285, 1429
BseLI	CCNNNNNNNGG	4 cut(s)	457, 704, 971, 985
BseMI	GCAATG	4 cut(s)	524, 1059, 1299, 1482
BseMII	CTCAG	1 cut(s)	17
BseNI	ACTGG	2 cut(s)	322, 990
BseXI	GCAGC	8 cut(s)	9, 27, 86, 92, 144, 878, 1183, 1211
BsgI	GTGCAG	1 cut(s)	151
Bsh1236I	CGCG	3 cut(s)	79, 844, 1455
BshFI	GGCC	2 cut(s)	989, 1320
BsiHKAI	GWGCWC	1 cut(s)	1479
BsiSI	CCGG	3 cut(s)	71, 165, 518
BslFI	GGGAC	4 cut(s)	457, 921, 1034, 1242
BslI	CCNNNNNNNGG	4 cut(s)	457, 704, 971, 985
BsmAI	GTCTC	1 cut(s)	327
BsmFI	GGGAC	4 cut(s)	457, 921, 1034, 1242
BsmI	GAATGC	2 cut(s)	790, 1238
BsnI	GGCC	2 cut(s)	989, 1320
Bsp1286I	GDGCHC	1 cut(s)	1479
Bsp143I	GATC	7 cut(s)	49, 141, 575, 763, 975, 1114, 1405
Bsp1720I	GCTNAGC	2 cut(s)	26, 1378
Bsp68I	TCGCGA	2 cut(s)	844, 1455
BspACI	CCGC	2 cut(s)	592, 931
BspANI	GGCC	2 cut(s)	989, 1320
BspCNI	CTCAG	1 cut(s)	18
BspFNI	CGCG	3 cut(s)	79, 844, 1455
BspLI	GGNNCC	5 cut(s)	516, 977, 1050, 1099, 1258
BspMAI	CTGCAG	2 cut(s)	860, 1201
BspMI	ACCTGC	2 cut(s)	824, 860
BspPI	GGATC	7 cut(s)	149, 583, 771, 970, 983, 1122, 1400
BspTI	CTTAAG	1 cut(s)	1153
BsrDI	GCAATG	4 cut(s)	524, 1059, 1299, 1482
BsrFI	RCCGGY	1 cut(s)	164
BsrI	ACTGG	2 cut(s)	322, 990
BssAI	RCCGGY	1 cut(s)	164
BssECI	CCNNGG	2 cut(s)	713, 1488
BssMI	GATC	7 cut(s)	49, 141, 575, 763, 975, 1114, 1405
BssT1I	CCWWGG	1 cut(s)	1488
Bst2UI	CCWGG	3 cut(s)	714, 980, 1046
Bst4CI	ACNGT	1 cut(s)	754
BstAFI	CTTAAG	1 cut(s)	1153
BstC8I	GCNNGC	2 cut(s)	20, 166
BstDEI	CTNAG	5 cut(s)	26, 65, 935, 1002, 1378
BstENI	CCTNNNNNAGG	1 cut(s)	702
BstF5I	GGATG	6 cut(s)	190, 319, 469, 526, 1285, 1429
BstFNI	CGCG	3 cut(s)	79, 844, 1455
BstH2I	RGCGCY	2 cut(s)	165, 739
BstHHI	GCGC	3 cut(s)	79, 164, 738
BstKTI	GATC	7 cut(s)	52, 144, 578, 766, 978, 1117, 1408
BstMAI	GTCTC	1 cut(s)	327
BstMBI	GATC	7 cut(s)	49, 141, 575, 763, 975, 1114, 1405
BstMWI	GCNNNNNNNGC	4 cut(s)	204, 742, 1193, 1205
BstNI	CCWGG	3 cut(s)	714, 980, 1046
BstNSI	RCATGY	1 cut(s)	1331
BstSCI	CCNGG	5 cut(s)	69, 516, 712, 978, 1044
BstSFI	CTRYAG	2 cut(s)	856, 1197
BstUI	CGCG	3 cut(s)	79, 844, 1455
BstV1I	GCAGC	8 cut(s)	9, 27, 86, 92, 144, 878, 1183, 1211
BstX2I	RGATCY	1 cut(s)	975
BstXI	CCANNNNNNTGG	1 cut(s)	673
BstYI	RGATCY	1 cut(s)	975
BsuI	GTATCC	2 cut(s)	243, 938
BsuRI	GGCC	2 cut(s)	989, 1320
BtgZI	GCGATG	1 cut(s)	1470
BtrI	CACGTC	1 cut(s)	471
BtsCI	GGATG	6 cut(s)	190, 319, 469, 526, 1285, 1429
BtsI	GCAGTG	1 cut(s)	441
BtsIMutI	CAGTG	1 cut(s)	441
BtuMI	TCGCGA	2 cut(s)	844, 1455
BveI	ACCTGC	2 cut(s)	824, 860
Cac8I	GCNNGC	2 cut(s)	20, 166
CaiI	CAGNNNCTG	1 cut(s)	985
CfoI	GCGC	3 cut(s)	79, 164, 738
Cfr10I	RCCGGY	1 cut(s)	164
Cfr13I	GGNCC	5 cut(s)	274, 514, 707, 1048, 1256
CseI	GACGC	1 cut(s)	68
Csp6I	GTAC	1 cut(s)	498
CviAII	CATG	6 cut(s)	278, 295, 478, 554, 796, 1328
CviQI	GTAC	1 cut(s)	498
DdeI	CTNAG	5 cut(s)	26, 65, 935, 1002, 1378
DpnI	GATC	7 cut(s)	51, 143, 577, 765, 977, 1116, 1407
DpnII	GATC	7 cut(s)	49, 141, 575, 763, 975, 1114, 1405
EaeI	YGGCCR	2 cut(s)	987, 1318
EciI	GGCGGA	1 cut(s)	920
Ecl136II	GAGCTC	1 cut(s)	1477
Eco130I	CCWWGG	1 cut(s)	1488
Eco24I	GRGCYC	1 cut(s)	1479
Eco47I	GGWCC	5 cut(s)	274, 514, 707, 1048, 1256
Eco47III	AGCGCT	1 cut(s)	737
Eco53kI	GAGCTC	1 cut(s)	1477
Eco57I	CTGAAG	1 cut(s)	171
EcoICRI	GAGCTC	1 cut(s)	1477
EcoNI	CCTNNNNNAGG	1 cut(s)	702
EcoRII	CCWGG	3 cut(s)	712, 978, 1044
EcoT14I	CCWWGG	1 cut(s)	1488
EcoT22I	ATGCAT	3 cut(s)	526, 1333, 1344
EcoT38I	GRGCYC	1 cut(s)	1479
ErhI	CCWWGG	1 cut(s)	1488
FaeI	CATG	6 cut(s)	281, 298, 481, 557, 799, 1331
FalI	AAGNNNNNCTT	4 cut(s)	164, 196, 725, 757
FaqI	GGGAC	4 cut(s)	457, 921, 1034, 1242
FatI	CATG	6 cut(s)	277, 294, 477, 553, 795, 1327
FauNDI	CATATG	1 cut(s)	1338
Fnu4HI	GCNGC	9 cut(s)	16, 23, 75, 106, 133, 867, 1197, 1200, 1504
FokI	GGATG	6 cut(s)	177, 326, 476, 533, 1272, 1436
FriOI	GRGCYC	1 cut(s)	1479
Fsp4HI	GCNGC	9 cut(s)	16, 23, 75, 106, 133, 867, 1197, 1200, 1504
FspBI	CTAG	3 cut(s)	204, 491, 531
GlaI	GCGC	3 cut(s)	78, 163, 737
GluI	GCNGC	9 cut(s)	16, 23, 75, 106, 133, 867, 1197, 1200, 1504
HaeII	RGCGCY	2 cut(s)	165, 739
HaeIII	GGCC	2 cut(s)	989, 1320
HapII	CCGG	3 cut(s)	71, 165, 518
HgaI	GACGC	1 cut(s)	68
HhaI	GCGC	3 cut(s)	79, 164, 738
Hin1II	CATG	6 cut(s)	281, 298, 481, 557, 799, 1331
Hin6I	GCGC	3 cut(s)	77, 162, 736
HinP1I	GCGC	3 cut(s)	77, 162, 736
HincII	GTYRAC	1 cut(s)	1360
HindII	GTYRAC	1 cut(s)	1360
HinfI	GANTC	6 cut(s)	299, 439, 445, 694, 1394, 1436
HpaII	CCGG	3 cut(s)	71, 165, 518
HphI	GGTGA	5 cut(s)	84, 226, 440, 884, 1048
Hpy166II	GTNNAC	3 cut(s)	710, 1315, 1360
Hpy188I	TCNGA	5 cut(s)	304, 418, 444, 1119, 1184
Hpy188III	TCNNGA	6 cut(s)	149, 365, 843, 1243, 1409, 1454
Hpy8I	GTNNAC	3 cut(s)	710, 1315, 1360
HpyAV	CCTTC	5 cut(s)	166, 211, 898, 1063, 1487
HpyCH4III	ACNGT	1 cut(s)	754
HpyCH4IV	ACGT	3 cut(s)	405, 470, 1356
HpyF10VI	GCNNNNNNNGC	4 cut(s)	204, 742, 1193, 1205
HpyF3I	CTNAG	5 cut(s)	26, 65, 935, 1002, 1378
HpySE526I	ACGT	3 cut(s)	405, 470, 1356
Hsp92II	CATG	6 cut(s)	281, 298, 481, 557, 799, 1331
HspAI	GCGC	3 cut(s)	77, 162, 736
KroI	GCCGGC	1 cut(s)	164
KroNI	GCCGGC	1 cut(s)	166
Kzo9I	GATC	7 cut(s)	49, 141, 575, 763, 975, 1114, 1405
LmnI	GCTCC	6 cut(s)	893, 1097, 1184, 1193, 1309, 1474
Lsp1109I	GCAGC	8 cut(s)	9, 27, 86, 92, 144, 878, 1183, 1211
LweI	GCATC	7 cut(s)	199, 304, 409, 511, 754, 1294, 1447
MaeI	CTAG	3 cut(s)	204, 491, 531
MaeII	ACGT	3 cut(s)	405, 470, 1356
MaeIII	GTNAC	4 cut(s)	480, 619, 823, 1352
MalI	GATC	7 cut(s)	51, 143, 577, 765, 977, 1116, 1407
MboI	GATC	7 cut(s)	49, 141, 575, 763, 975, 1114, 1405
MboII	GAAGA	7 cut(s)	59, 558, 793, 913, 1277, 1445, 1509
MflI	RGATCY	1 cut(s)	975
MhlI	GDGCHC	1 cut(s)	1479
MluCI	AATT	5 cut(s)	227, 955, 1225, 1269, 1290
MlyI	GAGTC	1 cut(s)	688
MmeI	TCCRAC	2 cut(s)	563, 1401
Mph1103I	ATGCAT	3 cut(s)	526, 1333, 1344
MroNI	GCCGGC	1 cut(s)	164
MseI	TTAA	4 cut(s)	702, 954, 1154, 1169
MslI	CAYNNNNRTG	2 cut(s)	122, 1326
MspA1I	CMGCKG	3 cut(s)	105, 968, 1506
MspCI	CTTAAG	1 cut(s)	1153
MspI	CCGG	3 cut(s)	71, 165, 518
MspR9I	CCNGG	5 cut(s)	71, 518, 714, 980, 1046
Mva1269I	GAATGC	2 cut(s)	790, 1238
MvaI	CCWGG	3 cut(s)	714, 980, 1046
MvnI	CGCG	3 cut(s)	79, 844, 1455
MwoI	GCNNNNNNNGC	4 cut(s)	204, 742, 1193, 1205
NaeI	GCCGGC	1 cut(s)	166
NciI	CCSGG	2 cut(s)	71, 518
NdeI	CATATG	1 cut(s)	1338
NdeII	GATC	7 cut(s)	49, 141, 575, 763, 975, 1114, 1405
NgoMIV	GCCGGC	1 cut(s)	164
NlaIII	CATG	6 cut(s)	281, 298, 481, 557, 799, 1331
NlaIV	GGNNCC	5 cut(s)	516, 977, 1050, 1099, 1258
NmuCI	GTSAC	2 cut(s)	480, 823
NruI	TCGCGA	2 cut(s)	844, 1455
NsiI	ATGCAT	3 cut(s)	526, 1333, 1344
NspI	RCATGY	1 cut(s)	1331
PaqCI	CACCTGC	1 cut(s)	860
PctI	GAATGC	2 cut(s)	790, 1238
PdiI	GCCGGC	1 cut(s)	166
PfeI	GAWTC	5 cut(s)	299, 439, 445, 1394, 1436
PflMI	CCANNNNNTGG	3 cut(s)	457, 971, 985
PfoI	TCCNGGA	1 cut(s)	978
PkrI	GCNGC	9 cut(s)	17, 24, 76, 107, 134, 868, 1198, 1201, 1505
PleI	GAGTC	1 cut(s)	688
PpsI	GAGTC	1 cut(s)	688
Psp124BI	GAGCTC	1 cut(s)	1479
Psp6I	CCWGG	3 cut(s)	712, 978, 1044
PspGI	CCWGG	3 cut(s)	712, 978, 1044
PspN4I	GGNNCC	5 cut(s)	516, 977, 1050, 1099, 1258
PspPI	GGNCC	5 cut(s)	274, 514, 707, 1048, 1256
PstI	CTGCAG	2 cut(s)	860, 1201
PstNI	CAGNNNCTG	1 cut(s)	985
PsuI	RGATCY	1 cut(s)	975
PvuII	CAGCTG	3 cut(s)	105, 968, 1506
RruI	TCGCGA	2 cut(s)	844, 1455
RsaI	GTAC	1 cut(s)	499
RsaNI	GTAC	1 cut(s)	498
RseI	CAYNNNNRTG	2 cut(s)	122, 1326
SacI	GAGCTC	1 cut(s)	1479
SaqAI	TTAA	4 cut(s)	702, 954, 1154, 1169
SatI	GCNGC	9 cut(s)	16, 23, 75, 106, 133, 867, 1197, 1200, 1504
Sau3AI	GATC	7 cut(s)	49, 141, 575, 763, 975, 1114, 1405
Sau96I	GGNCC	5 cut(s)	274, 514, 707, 1048, 1256
SchI	GAGTC	1 cut(s)	688
ScrFI	CCNGG	5 cut(s)	71, 518, 714, 980, 1046
SduI	GDGCHC	1 cut(s)	1479
SfaNI	GCATC	7 cut(s)	199, 304, 409, 511, 754, 1294, 1447
SfcI	CTRYAG	2 cut(s)	856, 1197
SinI	GGWCC	5 cut(s)	274, 514, 707, 1048, 1256
SmiMI	CAYNNNNRTG	2 cut(s)	122, 1326
SmlI	CTYRAG	1 cut(s)	1153
SmoI	CTYRAG	1 cut(s)	1153
Sse9I	AATT	5 cut(s)	227, 955, 1225, 1269, 1290
SsiI	CCGC	2 cut(s)	592, 931
SspI	AATATT	1 cut(s)	946
SspMI	CTAG	3 cut(s)	204, 491, 531
SstI	GAGCTC	1 cut(s)	1479
StyD4I	CCNGG	5 cut(s)	69, 516, 712, 978, 1044
StyI	CCWWGG	1 cut(s)	1488
TaaI	ACNGT	1 cut(s)	754
TaiI	ACGT	3 cut(s)	408, 473, 1359
TaqI	TCGA	5 cut(s)	455, 578, 748, 1263, 1467
TasI	AATT	5 cut(s)	227, 955, 1225, 1269, 1290
TfiI	GAWTC	5 cut(s)	299, 439, 445, 1394, 1436
Tru1I	TTAA	4 cut(s)	702, 954, 1154, 1169
Tru9I	TTAA	4 cut(s)	702, 954, 1154, 1169
TscAI	CASTG	1 cut(s)	441
TseFI	GTSAC	2 cut(s)	480, 823
TseI	GCWGC	9 cut(s)	15, 22, 74, 105, 132, 866, 1196, 1199, 1503
Tsp45I	GTSAC	2 cut(s)	480, 823
TspDTI	ATGAA	1 cut(s)	806
TspRI	CASTG	1 cut(s)	441
Van91I	CCANNNNNTGG	3 cut(s)	457, 971, 985
Vha464I	CTTAAG	1 cut(s)	1153
VpaK11BI	GGWCC	5 cut(s)	274, 514, 707, 1048, 1256
XagI	CCTNNNNNAGG	1 cut(s)	702
XceI	RCATGY	1 cut(s)	1331
XcmI	CCANNNNNNNNNTGG	2 cut(s)	508, 1108
XspI	CTAG	3 cut(s)	204, 491, 531
Zsp2I	ATGCAT	3 cut(s)	526, 1333, 1344

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.