Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002697

Physical Location & Seq

Genomic Coordinates Forward (+)

3005603 .. 3009360

Gene Structure 3758 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1040600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1167 bp

ATGGATCTGAGAGAAAGCATCATCAACCAAAACAACTTCGCTCTGGGGCTGACGAAGCAGCTGCTTCAGACCGAAGGCAAGCAATCGAACCTCGTGTACTCGCCGCTGTCCATCCACGTGCTGTTGAGCCTGATCGCGGCCGGGTCAAAGGGTCCGACTCAGGACCAGCTGCTGTCTTTTCTCCAGTCAAAGTCCACCGACCACCTCAGCTCCTTCGCCGCCGAGCTCGTCTCTGTGATTTTCTCCGATGGGTCCCCTAGCGGCGGCCCCCAGCTGTCGTTTGCAAATGGTGGTTGGGTGGACAGGCGTCTCCCTCTGAAGCCATCTTTCAAGCAGGTGGTGGAAACTTCTTACATGGCCGCTCTCACCCAAGTCGATTTTCAGACCAATGCTGCTCAAGTGGCCTCGGGAGTGAATTCATGGGCTGAAAAGGAGACGAGAGGCCTTATCAAGGAGGTACTTCCTCCTGGGTCAGTTGACTGCTCAACCACGCTCATCTTTGCAAATGCGTTATACTTCAAAGGAGCTTGGACTGAAAAGTTTGATGCATCACAAACGAAAGAGCATGATTTCCACCTTCTCGATGGGAGCGCAGTTAAGGTGCCCTTCATGACCAACAGGAAGAAGCAGTGTGTAAGATCCTTCAATGGTTTCTCAGTCTTAGGGCTTCATTACAAGCAAGGTGAAGACAAGCGATGTTTCTCCATGCACATCTTTCTTCCAGAGGCGAAAGATGGGCTGCCAGCTTTGGTTGAGAAACTTGGTTCTGAGTCCGGGTTTTTGGATCGCCATCTTCCCAAGCAAAAAATTGCCGTGGGTAATTTCAGGATTCCAAAGTTTAAGATTTCATTTGGGTTTGAAGCTTCGAATGTCCTTAAAGGTCTGGGCTTGGTGCTGCCATTCGGTGGTGGAGGAGGTTTGACAGAGATGGTGGACTCGCCTGAGGGTAAGAACTTATACGTGTCCAGCATTTTCCATAAGTCGTTCATCGAAGTGAACGAAGAAGGCACGGAAGCCGCAGCTGCTTCCGCCTTTCGTAAAACTATGCTGTGTCTGCCGAGGGCGGTTGACTTTGTGGCTGATCGTCCATTCCTCTTCTTGGTCAAGGAAGAATTGACCGGAGCCGTGCTGTTCATCGGCCATGTACTCAATCCTCTTGCAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0004857 GO:0004866 GO:0004867 GO:0004869 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0009892 GO:0010466 GO:0010605 GO:0010951 GO:0019222 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0043086 GO:0044092 GO:0044421 GO:0044424 GO:0044464 GO:0045861 GO:0048046 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko05146 map05146

Pfam Domains

Protein Families

Serpin

Protein Analysis

389

Amino Acids

42.22

Weight (kDa)

7.7

Isoelectric Point (pI)

38.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Serpin	PF00079	9 - 385	3.2e-96	Serpin (serine protease inhibitor)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002697)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47710 AT1G47710 AT1G51330 AT1G62160 AT1G62170 AT1G62170 AT1G63280 AT1G64010 AT1G64010 AT1G64030 AT2G14540 AT2G25240 AT2G26390 AT2G35580 AT3G45220
fragaria_vesca	FvH4_2g40700
malus_domestica	MD08G1040600.v1.1 MD08G1041200.v1.1 MD08G1041300.v1.1 MD08G1041500.v1.1 MD08G1041600.v1.1 MD14G1097300.v1.1 MD15G1035000.v1.1 MD15G1286900.v1.1
prunus_persica	Prupe.1G389200_v2.0.a1 Prupe.1G389300_v2.0.a1 Prupe.3G141200_v2.0.a1
pyrus_communis	pycom08g03320 pycom08g03330 pycom08g03340 pycom08g03360 pycom15g03220
rosa_chinensis	RchiOBHm_Chr6g0299901
rosa_laevigata	RLG00000011377
rosa_multiflora	Rmu_ssc0000158.1_g000016
rosa_roxburghii	Rroxscaffold_7G00168290
rosa_rugosa	Rorug06G0293100
rosa_samantha	Rh4AG219100
rosa_wichuraiana	Rw6G035390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	325
Acc36I	ACCTGC	1 cut(s)	325
AccB1I	GGYRCC	1 cut(s)	601
AccB7I	CCANNNNNTGG	1 cut(s)	905
AccBSI	CCGCTC	1 cut(s)	362
AccII	CGCG	1 cut(s)	137
AciI	CCGC	9 cut(s)	104, 137, 219, 261, 264, 360, 1017, 1029, 1064
AclWI	GGATC	3 cut(s)	12, 633, 792
AcoI	YGGCCR	3 cut(s)	138, 357, 1138
AcsI	RAATTY	1 cut(s)	415
AcuI	CTGAAG	2 cut(s)	50, 338
AcvI	CACGTG	1 cut(s)	118
AcyI	GRCGYC	1 cut(s)	307
AfaI	GTAC	3 cut(s)	98, 459, 1146
AfiI	CCNNNNNNNGG	6 cut(s)	136, 263, 451, 905, 1099, 1160
AflIII	ACRYGT	1 cut(s)	960
AgsI	TTSAA	4 cut(s)	331, 520, 646, 860
AjnI	CCWGG	1 cut(s)	466
AjuI	GAANNNNNNNTTGG	2 cut(s)	363, 395
AluBI	AGCT	9 cut(s)	61, 169, 210, 226, 274, 527, 746, 863, 1022
AluI	AGCT	9 cut(s)	61, 169, 210, 226, 274, 527, 746, 863, 1022
Alw21I	GWGCWC	1 cut(s)	228
Alw26I	GTCTC	3 cut(s)	235, 314, 428
AlwI	GGATC	3 cut(s)	12, 633, 792
AlwNI	CAGNNNCTG	1 cut(s)	172
Ama87I	CYCGRG	1 cut(s)	406
AoxI	GGCC	6 cut(s)	138, 265, 357, 402, 442, 1138
ApeKI	GCWGC	8 cut(s)	58, 61, 169, 392, 739, 895, 1019, 1022
ApoI	RAATTY	1 cut(s)	415
AspLEI	GCGC	1 cut(s)	593
AspS9I	GGNCC	4 cut(s)	152, 163, 252, 266
AsuC2I	CCSGG	2 cut(s)	142, 775
AsuHPI	GGTGA	2 cut(s)	358, 695
AsuII	TTCGAA	1 cut(s)	866
AvaI	CYCGRG	1 cut(s)	406
AvaII	GGWCC	3 cut(s)	152, 163, 252
AxyI	CCTNAGG	1 cut(s)	942
BaeGI	GKGCMC	1 cut(s)	606
BanI	GGYRCC	1 cut(s)	601
BanII	GRGCYC	1 cut(s)	228
BauI	CACGAG	1 cut(s)	92
BbrPI	CACGTG	1 cut(s)	118
BbsI	GAAGAC	1 cut(s)	693
Bbv12I	GWGCWC	1 cut(s)	228
BbvCI	CCTCAGC	1 cut(s)	206
BbvI	GCAGC	8 cut(s)	48, 70, 156, 379, 726, 882, 1009, 1031
BccI	CCATC	7 cut(s)	119, 242, 331, 578, 728, 798, 922
BceAI	ACGGC	2 cut(s)	797, 1109
BcgI	CGANNNNNNTGC	2 cut(s)	43, 77
BciT130I	CCWGG	1 cut(s)	468
BcnI	CCSGG	2 cut(s)	142, 775
BcoDI	GTCTC	3 cut(s)	235, 314, 428
BfaI	CTAG	1 cut(s)	258
BfuAI	ACCTGC	1 cut(s)	325
Bme1390I	CCNGG	3 cut(s)	142, 468, 775
Bme18I	GGWCC	3 cut(s)	152, 163, 252
BmeT110I	CYCGRG	1 cut(s)	406
BmgT120I	GGNCC	4 cut(s)	152, 163, 252, 266
BmiI	GGNNCC	6 cut(s)	153, 253, 254, 268, 603, 1123
BmrFI	CCNGG	3 cut(s)	142, 468, 775
BmsI	GCATC	3 cut(s)	27, 535, 557
BoxI	GACNNNNGTC	1 cut(s)	306
BpiI	GAAGAC	1 cut(s)	693
BplI	GAGNNNNNCTC	2 cut(s)	215, 247
BpmI	CTGGAG	1 cut(s)	167
Bpu10I	CCTNAGC	1 cut(s)	206
Bpu14I	TTCGAA	1 cut(s)	866
BpuEI	CTTGAG	1 cut(s)	381
BpuMI	CCSGG	2 cut(s)	142, 775
BsaAI	YACGTR	2 cut(s)	118, 961
BsaBI	GATNNNNATC	1 cut(s)	789
BsaHI	GRCGYC	1 cut(s)	307
BsaJI	CCNNGG	4 cut(s)	405, 467, 813, 1058
BsaWI	WCCGGW	1 cut(s)	1118
BsaXI	ACNNNNNCTCC	2 cut(s)	194, 224
Bsc4I	CCNNNNNNNGG	6 cut(s)	136, 263, 451, 905, 1099, 1160
Bse1I	ACTGG	1 cut(s)	184
Bse21I	CCTNAGG	1 cut(s)	942
Bse8I	GATNNNNATC	1 cut(s)	789
BseBI	CCWGG	1 cut(s)	468
BseDI	CCNNGG	4 cut(s)	405, 467, 813, 1058
BseGI	GGATG	1 cut(s)	111
BseJI	GATNNNNATC	1 cut(s)	789
BseLI	CCNNNNNNNGG	6 cut(s)	136, 263, 451, 905, 1099, 1160
BseMII	CTCAG	5 cut(s)	173, 220, 669, 759, 933
BseNI	ACTGG	1 cut(s)	184
BseRI	GAGGAG	1 cut(s)	927
BseSI	GKGCMC	1 cut(s)	606
BseX3I	CGGCCG	1 cut(s)	138
BseXI	GCAGC	8 cut(s)	48, 70, 156, 379, 726, 882, 1009, 1031
BseYI	CCCAGC	1 cut(s)	270
Bsh1236I	CGCG	1 cut(s)	137
Bsh1285I	CGRYCG	1 cut(s)	141
BshFI	GGCC	6 cut(s)	140, 267, 359, 404, 444, 1140
BshNI	GGYRCC	1 cut(s)	601
BsiEI	CGRYCG	1 cut(s)	141
BsiHKAI	GWGCWC	1 cut(s)	228
BsiHKCI	CYCGRG	1 cut(s)	406
BsiSI	CCGG	3 cut(s)	141, 774, 1119
BslFI	GGGAC	1 cut(s)	238
BslI	CCNNNNNNNGG	6 cut(s)	136, 263, 451, 905, 1099, 1160
BsmAI	GTCTC	3 cut(s)	235, 314, 428
BsmBI	CGTCTC	3 cut(s)	235, 314, 428
BsmFI	GGGAC	1 cut(s)	238
BsnI	GGCC	6 cut(s)	140, 267, 359, 404, 444, 1140
BsoBI	CYCGRG	1 cut(s)	406
Bsp119I	TTCGAA	1 cut(s)	866
Bsp1286I	GDGCHC	2 cut(s)	228, 606
Bsp143I	GATC	5 cut(s)	4, 132, 638, 784, 1081
BspACI	CCGC	9 cut(s)	104, 137, 219, 261, 264, 360, 1017, 1029, 1064
BspANI	GGCC	6 cut(s)	140, 267, 359, 404, 444, 1140
BspCNI	CTCAG	5 cut(s)	172, 219, 668, 760, 934
BspFNI	CGCG	1 cut(s)	137
BspHI	TCATGA	1 cut(s)	609
BspLI	GGNNCC	6 cut(s)	153, 253, 254, 268, 603, 1123
BspMI	ACCTGC	1 cut(s)	325
BspPI	GGATC	3 cut(s)	12, 633, 792
BspT104I	TTCGAA	1 cut(s)	866
BspT107I	GGYRCC	1 cut(s)	601
BsrBI	CCGCTC	1 cut(s)	362
BsrI	ACTGG	1 cut(s)	184
BssECI	CCNNGG	4 cut(s)	405, 467, 813, 1058
BssMI	GATC	5 cut(s)	4, 132, 638, 784, 1081
BssNI	GRCGYC	1 cut(s)	307
BssSI	CACGAG	1 cut(s)	92
Bst2BI	CACGAG	1 cut(s)	92
Bst2UI	CCWGG	1 cut(s)	468
Bst6I	CTCTTC	1 cut(s)	1100
BstACI	GRCGYC	1 cut(s)	307
BstBAI	YACGTR	2 cut(s)	118, 961
BstBI	TTCGAA	1 cut(s)	866
BstC8I	GCNNGC	3 cut(s)	80, 744, 1162
BstDEI	CTNAG	7 cut(s)	8, 159, 206, 655, 661, 768, 942
BstDSI	CCRYGG	1 cut(s)	813
BstENI	CCTNNNNNAGG	2 cut(s)	449, 1158
BstF5I	GGATG	1 cut(s)	111
BstFNI	CGCG	1 cut(s)	137
BstHHI	GCGC	1 cut(s)	593
BstKTI	GATC	5 cut(s)	7, 135, 641, 787, 1084
BstMAI	GTCTC	3 cut(s)	235, 314, 428
BstMBI	GATC	5 cut(s)	4, 132, 638, 784, 1081
BstMCI	CGRYCG	1 cut(s)	141
BstMWI	GCNNNNNNNGC	5 cut(s)	55, 401, 1022, 1028, 1054
BstNI	CCWGG	1 cut(s)	468
BstPAI	GACNNNNGTC	1 cut(s)	306
BstSCI	CCNGG	3 cut(s)	140, 466, 773
BstSLI	GKGCMC	1 cut(s)	606
BstUI	CGCG	1 cut(s)	137
BstV1I	GCAGC	8 cut(s)	48, 70, 156, 379, 726, 882, 1009, 1031
BstV2I	GAAGAC	1 cut(s)	693
BstX2I	RGATCY	2 cut(s)	4, 638
BstYI	RGATCY	2 cut(s)	4, 638
BstZI	CGGCCG	1 cut(s)	138
Bsu36I	CCTNAGG	1 cut(s)	942
BsuRI	GGCC	6 cut(s)	140, 267, 359, 404, 444, 1140
BtgI	CCRYGG	1 cut(s)	813
BtgZI	GCGATG	1 cut(s)	709
BtsCI	GGATG	1 cut(s)	111
BtsI	GCAGTG	1 cut(s)	635
BtsIMutI	CAGTG	1 cut(s)	635
BveI	ACCTGC	1 cut(s)	325
Cac8I	GCNNGC	3 cut(s)	80, 744, 1162
CaiI	CAGNNNCTG	1 cut(s)	172
CciI	TCATGA	1 cut(s)	609
CfoI	GCGC	1 cut(s)	593
Cfr13I	GGNCC	4 cut(s)	152, 163, 252, 266
CseI	GACGC	1 cut(s)	296
Csp6I	GTAC	3 cut(s)	97, 458, 1145
CviAII	CATG	6 cut(s)	355, 420, 566, 610, 706, 1142
CviQI	GTAC	3 cut(s)	97, 458, 1145
DdeI	CTNAG	7 cut(s)	8, 159, 206, 655, 661, 768, 942
DpnI	GATC	5 cut(s)	6, 134, 640, 786, 1083
DpnII	GATC	5 cut(s)	4, 132, 638, 784, 1081
EaeI	YGGCCR	3 cut(s)	138, 357, 1138
EagI	CGGCCG	1 cut(s)	138
Eam1104I	CTCTTC	1 cut(s)	1100
EarI	CTCTTC	1 cut(s)	1100
EciI	GGCGGA	1 cut(s)	1018
Ecl136II	GAGCTC	1 cut(s)	226
EclXI	CGGCCG	1 cut(s)	138
Eco147I	AGGCCT	1 cut(s)	444
Eco24I	GRGCYC	1 cut(s)	228
Eco47I	GGWCC	3 cut(s)	152, 163, 252
Eco52I	CGGCCG	1 cut(s)	138
Eco53kI	GAGCTC	1 cut(s)	226
Eco57I	CTGAAG	2 cut(s)	50, 338
Eco72I	CACGTG	1 cut(s)	118
Eco81I	CCTNAGG	1 cut(s)	942
Eco88I	CYCGRG	1 cut(s)	406
EcoICRI	GAGCTC	1 cut(s)	226
EcoNI	CCTNNNNNAGG	2 cut(s)	449, 1158
EcoO109I	RGGNCCY	1 cut(s)	252
EcoRI	GAATTC	1 cut(s)	415
EcoRII	CCWGG	1 cut(s)	466
EcoT22I	ATGCAT	1 cut(s)	550
EcoT38I	GRGCYC	1 cut(s)	228
Esp3I	CGTCTC	3 cut(s)	235, 314, 428
FaeI	CATG	6 cut(s)	358, 423, 569, 613, 709, 1145
FalI	AAGNNNNNCTT	2 cut(s)	590, 622
FaqI	GGGAC	1 cut(s)	238
FatI	CATG	6 cut(s)	354, 419, 565, 609, 705, 1141
FokI	GGATG	1 cut(s)	98
FriOI	GRGCYC	1 cut(s)	228
FspBI	CTAG	1 cut(s)	258
GlaI	GCGC	1 cut(s)	592
GsaI	CCCAGC	1 cut(s)	274
GsuI	CTGGAG	1 cut(s)	167
HaeIII	GGCC	6 cut(s)	140, 267, 359, 404, 444, 1140
HapII	CCGG	3 cut(s)	141, 774, 1119
HgaI	GACGC	1 cut(s)	296
HhaI	GCGC	1 cut(s)	593
Hin1I	GRCGYC	1 cut(s)	307
Hin1II	CATG	6 cut(s)	358, 423, 569, 613, 709, 1145
Hin6I	GCGC	1 cut(s)	591
HinP1I	GCGC	1 cut(s)	591
HincII	GTYRAC	2 cut(s)	478, 1069
HindII	GTYRAC	2 cut(s)	478, 1069
HindIII	AAGCTT	1 cut(s)	861
HinfI	GANTC	4 cut(s)	157, 770, 829, 935
HpaII	CCGG	3 cut(s)	141, 774, 1119
HphI	GGTGA	2 cut(s)	358, 695
Hpy166II	GTNNAC	7 cut(s)	97, 195, 301, 478, 934, 997, 1069
Hpy188I	TCNGA	7 cut(s)	9, 69, 156, 247, 318, 384, 769
Hpy188III	TCNNGA	6 cut(s)	161, 408, 581, 610, 722, 826
Hpy8I	GTNNAC	7 cut(s)	97, 195, 301, 478, 934, 997, 1069
HpyAV	CCTTC	6 cut(s)	68, 223, 587, 616, 652, 998
HpyCH4IV	ACGT	2 cut(s)	117, 960
HpyCH4V	TGCA	5 cut(s)	284, 503, 548, 709, 1160
HpyF10VI	GCNNNNNNNGC	5 cut(s)	55, 401, 1022, 1028, 1054
HpyF3I	CTNAG	7 cut(s)	8, 159, 206, 655, 661, 768, 942
HpySE526I	ACGT	2 cut(s)	117, 960
Hsp92I	GRCGYC	1 cut(s)	307
Hsp92II	CATG	6 cut(s)	358, 423, 569, 613, 709, 1145
HspAI	GCGC	1 cut(s)	591
KflI	GGGWCCC	1 cut(s)	252
Kzo9I	GATC	5 cut(s)	4, 132, 638, 784, 1081
LmnI	GCTCC	4 cut(s)	215, 524, 588, 1121
Lsp1109I	GCAGC	8 cut(s)	48, 70, 156, 379, 726, 882, 1009, 1031
LweI	GCATC	3 cut(s)	27, 535, 557
MaeI	CTAG	1 cut(s)	258
MaeII	ACGT	2 cut(s)	117, 960
MalI	GATC	5 cut(s)	6, 134, 640, 786, 1083
MbiI	CCGCTC	1 cut(s)	362
MboI	GATC	5 cut(s)	4, 132, 638, 784, 1081
MboII	GAAGA	7 cut(s)	634, 698, 710, 785, 1013, 1087, 1121
MflI	RGATCY	2 cut(s)	4, 638
MhlI	GDGCHC	2 cut(s)	228, 606
MluCI	AATT	4 cut(s)	415, 807, 820, 1112
MlyI	GAGTC	3 cut(s)	151, 779, 929
MmeI	TCCRAC	1 cut(s)	179
Mph1103I	ATGCAT	1 cut(s)	550
MseI	TTAA	3 cut(s)	597, 840, 876
MslI	CAYNNNNRTG	2 cut(s)	116, 992
MspA1I	CMGCKG	5 cut(s)	61, 106, 169, 274, 1022
MspI	CCGG	3 cut(s)	141, 774, 1119
MspR9I	CCNGG	3 cut(s)	142, 468, 775
MvaI	CCWGG	1 cut(s)	468
MvnI	CGCG	1 cut(s)	137
MwoI	GCNNNNNNNGC	5 cut(s)	55, 401, 1022, 1028, 1054
NciI	CCSGG	2 cut(s)	142, 775
NdeII	GATC	5 cut(s)	4, 132, 638, 784, 1081
NlaIII	CATG	6 cut(s)	358, 423, 569, 613, 709, 1145
NlaIV	GGNNCC	6 cut(s)	153, 253, 254, 268, 603, 1123
NmeAIII	GCCGAG	2 cut(s)	247, 1083
NsiI	ATGCAT	1 cut(s)	550
NspV	TTCGAA	1 cut(s)	866
PagI	TCATGA	1 cut(s)	609
PaqCI	CACCTGC	1 cut(s)	325
PceI	AGGCCT	1 cut(s)	444
PcsI	WCGNNNNNNNCGW	1 cut(s)	996
PfeI	GAWTC	1 cut(s)	829
PflMI	CCANNNNNTGG	1 cut(s)	905
PleI	GAGTC	3 cut(s)	151, 778, 929
PmaCI	CACGTG	1 cut(s)	118
PmlI	CACGTG	1 cut(s)	118
PpsI	GAGTC	3 cut(s)	151, 778, 929
Ppu21I	YACGTR	2 cut(s)	118, 961
PpuMI	RGGWCCY	1 cut(s)	252
PshAI	GACNNNNGTC	1 cut(s)	306
Psp124BI	GAGCTC	1 cut(s)	228
Psp5II	RGGWCCY	1 cut(s)	252
Psp6I	CCWGG	1 cut(s)	466
PspCI	CACGTG	1 cut(s)	118
PspFI	CCCAGC	1 cut(s)	270
PspGI	CCWGG	1 cut(s)	466
PspN4I	GGNNCC	6 cut(s)	153, 253, 254, 268, 603, 1123
PspPI	GGNCC	4 cut(s)	152, 163, 252, 266
PspPPI	RGGWCCY	1 cut(s)	252
PsrI	GAACNNNNNNTAC	2 cut(s)	80, 112
PstNI	CAGNNNCTG	1 cut(s)	172
PsuI	RGATCY	2 cut(s)	4, 638
PvuII	CAGCTG	4 cut(s)	61, 169, 274, 1022
RsaI	GTAC	3 cut(s)	98, 459, 1146
RsaNI	GTAC	3 cut(s)	97, 458, 1145
RseI	CAYNNNNRTG	2 cut(s)	116, 992
SacI	GAGCTC	1 cut(s)	228
SaqAI	TTAA	3 cut(s)	597, 840, 876
Sau3AI	GATC	5 cut(s)	4, 132, 638, 784, 1081
Sau96I	GGNCC	4 cut(s)	152, 163, 252, 266
SchI	GAGTC	3 cut(s)	151, 779, 929
ScrFI	CCNGG	3 cut(s)	142, 468, 775
SduI	GDGCHC	2 cut(s)	228, 606
SfaNI	GCATC	3 cut(s)	27, 535, 557
SfuI	TTCGAA	1 cut(s)	866
SinI	GGWCC	3 cut(s)	152, 163, 252
SmiMI	CAYNNNNRTG	2 cut(s)	116, 992
SmlI	CTYRAG	1 cut(s)	396
SmoI	CTYRAG	1 cut(s)	396
Sse9I	AATT	4 cut(s)	415, 807, 820, 1112
SseBI	AGGCCT	1 cut(s)	444
SsiI	CCGC	9 cut(s)	104, 137, 219, 261, 264, 360, 1017, 1029, 1064
SspMI	CTAG	1 cut(s)	258
SstI	GAGCTC	1 cut(s)	228
StuI	AGGCCT	1 cut(s)	444
StyD4I	CCNGG	3 cut(s)	140, 466, 773
TaiI	ACGT	2 cut(s)	120, 963
TaqI	TCGA	5 cut(s)	86, 375, 582, 866, 990
TaqII	GACCGA	1 cut(s)	86
TasI	AATT	4 cut(s)	415, 807, 820, 1112
TatI	WGTACW	2 cut(s)	96, 1144
TauI	GCSGC	7 cut(s)	106, 140, 221, 264, 267, 362, 1019
TfiI	GAWTC	1 cut(s)	829
Tru1I	TTAA	3 cut(s)	597, 840, 876
Tru9I	TTAA	3 cut(s)	597, 840, 876
TscAI	CASTG	1 cut(s)	635
TseI	GCWGC	8 cut(s)	58, 61, 169, 392, 739, 895, 1019, 1022
TspDTI	ATGAA	6 cut(s)	408, 598, 659, 837, 976, 1123
TspGWI	ACGGA	1 cut(s)	1025
TspRI	CASTG	1 cut(s)	635
Van91I	CCANNNNNTGG	1 cut(s)	905
VpaK11BI	GGWCC	3 cut(s)	152, 163, 252
XagI	CCTNNNNNAGG	2 cut(s)	449, 1158
XapI	RAATTY	1 cut(s)	415
XcmI	CCANNNNNNNNNTGG	1 cut(s)	581
XspI	CTAG	1 cut(s)	258
Zsp2I	ATGCAT	1 cut(s)	550

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.