MD08G1041200.v1.1

Belongs to the serpin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002697
Physical Location & Seq
Forward (+)
3045254 .. 3046765
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1041200.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGGATCTGAGAGAAAGCGTCATCAACCAAAACGACTTCGCTCTGGGGCTGACGAAGCAGCTGCTTCAGACCGAAGGCAAGCAATCGAACCTCGTGTACTCGCCGGTGTCCATCCACGTGCTGTTGAGCCTGATCGCGGCCGGCTCAAAGGGTCCGACTCAGGACCAGCTGCTGTCTTTTCTCAAATCAAAGTCCACCGACCACCTCAACTCCTTCGCCGCCGAGCTCGTGTCCATGATCTTCTCCGATGGGTCCCCTAGCGGCGGCCCCCAGCTGTCGTTTGCTAATGGGGTTTGGGTGGACGGGTGTCTCCCTCTGAAGCCTTCTTTTAAGCAGGTGGTGGACACCTCTTACATGGCTGCTCTTGCCCAAGTCGATTTTCAGACCAATGCTGCTCAAGTGGCCTCGGGAGTGAATTCATGGGCTGAAAAGGAGACGAGAGGCCTTATCAAGGAGGTACTTCCTCCCGGGTCAGTTGACAGCTCAACCAGGCTCATCTTTGCAAATGCGTTATACTTCAAAGGAGCTTGGACTGAAAAGTTTGATGCATCACAAACGAAAGAGCATGATTTCTACCTTCTCGATGGGAGCACAGTTAAGGTGGCCTTCATGACCAGCAGGAAGAAGCAGTATGTAAGATCCTTCAATGGTTTCTCAGTCTTAGGGCTTCATTACAAGCAAGGTGAAGACAAGCGATGTTTCTCCATGCACATGTTTCTCCCAGAGGCGAAAGATGGGCTGCCAGCTTTGGTTGAGAAACTTGGTTCTGAGTCCGGGTTTTTGGATCGCCATCTTCCTAACCAAAAATTTGCAGTGGGTGATTTCAGGATTCCAAAGTTTAAGATTTCATTTGGGTTTGAAGCTTCCAATGTCCTTAAAGGTCTTGGCTTGGTGCTGCCATTCGGTGGCGGAGGAGGTTTGACAGAGATGGTGGACTCGCCTGTGGGTAAGAACTTGTACGTTTCCGGCATATTCCATAAGTCGTTCATCGAAGTGAACGAAGAAGGCACAGAAGCTGCAGCTGTGTCTGCCGGGCGTGTTAGGCGTTTGCGTCTGGTAGTGCCAAGGACGGTTGACTTTGTGGCCGATCGTCCATTTCTCTTCTTGATCAAGGAAGAATTGACCGGAACTGTGCTTTTCATCGGGCACGTACTCAATCCTCTTGCAGACTGA

Protein Analysis

391

Amino Acids

42.56

Weight (kDa)

7.1

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 9 - 387 7.1e-95 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 325
Acc36I ACCTGC 1 cut(s) 325
AccB7I CCANNNNNTGG 1 cut(s) 905
AccII CGCG 1 cut(s) 137
AciI CCGC 5 cut(s) 137, 219, 261, 264, 909
AclWI GGATC 3 cut(s) 12, 633, 792
AcoI YGGCCR 2 cut(s) 138, 1083
AcsI RAATTY 2 cut(s) 415, 806
AcuI CTGAAG 2 cut(s) 50, 338
AcvI CACGTG 1 cut(s) 118
AfaI GTAC 4 cut(s) 98, 459, 959, 1152
AfiI CCNNNNNNNGG 4 cut(s) 136, 263, 451, 905
AflIII ACRYGT 1 cut(s) 711
AgsI TTSAA 3 cut(s) 520, 646, 860
AjnI CCWGG 1 cut(s) 488
AjuI GAANNNNNNNTTGG 2 cut(s) 363, 395
Alw21I GWGCWC 2 cut(s) 228, 593
Alw26I GTCTC 2 cut(s) 314, 428
AlwI GGATC 3 cut(s) 12, 633, 792
AlwNI CAGNNNCTG 2 cut(s) 172, 1016
Ama87I CYCGRG 2 cut(s) 406, 467
AoxI GGCC 6 cut(s) 138, 265, 402, 442, 603, 1083
ApeKI GCWGC 9 cut(s) 58, 61, 169, 359, 392, 739, 895, 1016, 1019
ApoI RAATTY 2 cut(s) 415, 806
AspS9I GGNCC 4 cut(s) 152, 163, 252, 266
AsuC2I CCSGG 4 cut(s) 468, 469, 775, 1033
AsuHPI GGTGA 2 cut(s) 695, 830
AvaI CYCGRG 2 cut(s) 406, 467
AvaII GGWCC 3 cut(s) 152, 163, 252
BaeGI GKGCMC 1 cut(s) 1149
BanII GRGCYC 1 cut(s) 228
BauI CACGAG 2 cut(s) 92, 227
BbrPI CACGTG 1 cut(s) 118
BbsI GAAGAC 1 cut(s) 693
Bbv12I GWGCWC 2 cut(s) 228, 593
BbvI GCAGC 9 cut(s) 48, 70, 156, 346, 379, 726, 882, 1003, 1031
BccI CCATC 6 cut(s) 119, 242, 578, 728, 798, 922
BcgI CGANNNNNNTGC 2 cut(s) 43, 77
BciT130I CCWGG 1 cut(s) 490
BclI TGATCA 1 cut(s) 1107
BcnI CCSGG 4 cut(s) 468, 469, 775, 1033
BcoDI GTCTC 2 cut(s) 314, 428
BfaI CTAG 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 1017
BfuAI ACCTGC 1 cut(s) 325
Bme1390I CCNGG 5 cut(s) 468, 469, 490, 775, 1033
Bme18I GGWCC 3 cut(s) 152, 163, 252
BmeT110I CYCGRG 2 cut(s) 406, 467
BmgT120I GGNCC 4 cut(s) 152, 163, 252, 266
BmiI GGNNCC 4 cut(s) 153, 253, 254, 268
BmrFI CCNGG 5 cut(s) 468, 469, 490, 775, 1033
BmsI GCATC 2 cut(s) 535, 557
BoxI GACNNNNGTC 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 693
BpuEI CTTGAG 1 cut(s) 381
BpuMI CCSGG 4 cut(s) 468, 469, 775, 1033
BsaAI YACGTR 2 cut(s) 118, 1150
BsaBI GATNNNNATC 1 cut(s) 789
BsaJI CCNNGG 3 cut(s) 405, 467, 1064
BsaWI WCCGGW 1 cut(s) 1124
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 4 cut(s) 136, 263, 451, 905
Bse118I RCCGGY 2 cut(s) 103, 140
Bse8I GATNNNNATC 1 cut(s) 789
BseBI CCWGG 1 cut(s) 490
BseDI CCNNGG 3 cut(s) 405, 467, 1064
BseGI GGATG 1 cut(s) 111
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 4 cut(s) 136, 263, 451, 905
BseMII CTCAG 3 cut(s) 173, 669, 759
BseRI GAGGAG 1 cut(s) 927
BseSI GKGCMC 1 cut(s) 1149
BseX3I CGGCCG 1 cut(s) 138
BseXI GCAGC 9 cut(s) 48, 70, 156, 346, 379, 726, 882, 1003, 1031
BseYI CCCAGC 1 cut(s) 270
Bsh1236I CGCG 1 cut(s) 137
Bsh1285I CGRYCG 2 cut(s) 141, 1090
BshFI GGCC 6 cut(s) 140, 267, 404, 444, 605, 1085
BsiEI CGRYCG 2 cut(s) 141, 1090
BsiHKAI GWGCWC 2 cut(s) 228, 593
BsiHKCI CYCGRG 2 cut(s) 406, 467
BsiSI CCGG 7 cut(s) 104, 141, 468, 774, 966, 1032, 1125
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 4 cut(s) 136, 263, 451, 905
BsmAI GTCTC 2 cut(s) 314, 428
BsmBI CGTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 6 cut(s) 140, 267, 404, 444, 605, 1085
BsoBI CYCGRG 2 cut(s) 406, 467
Bsp1286I GDGCHC 3 cut(s) 228, 593, 1149
Bsp143I GATC 7 cut(s) 4, 132, 237, 638, 784, 1087, 1107
BspACI CCGC 5 cut(s) 137, 219, 261, 264, 909
BspANI GGCC 6 cut(s) 140, 267, 404, 444, 605, 1085
BspCNI CTCAG 3 cut(s) 172, 668, 760
BspFNI CGCG 1 cut(s) 137
BspHI TCATGA 1 cut(s) 609
BspLI GGNNCC 4 cut(s) 153, 253, 254, 268
BspMAI CTGCAG 1 cut(s) 1021
BspMI ACCTGC 1 cut(s) 325
BspPI GGATC 3 cut(s) 12, 633, 792
BsrFI RCCGGY 2 cut(s) 103, 140
BssAI RCCGGY 2 cut(s) 103, 140
BssECI CCNNGG 3 cut(s) 405, 467, 1064
BssMI GATC 7 cut(s) 4, 132, 237, 638, 784, 1087, 1107
BssSI CACGAG 2 cut(s) 92, 227
BssT1I CCWWGG 1 cut(s) 1064
Bst2BI CACGAG 2 cut(s) 92, 227
Bst2UI CCWGG 1 cut(s) 490
Bst4CI ACNGT 3 cut(s) 595, 1072, 1132
Bst6I CTCTTC 1 cut(s) 1106
BstBAI YACGTR 2 cut(s) 118, 1150
BstC8I GCNNGC 3 cut(s) 80, 142, 744
BstDEI CTNAG 5 cut(s) 8, 159, 655, 661, 768
BstENI CCTNNNNNAGG 1 cut(s) 449
BstF5I GGATG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 137
BstKTI GATC 7 cut(s) 7, 135, 240, 641, 787, 1090, 1110
BstMAI GTCTC 2 cut(s) 314, 428
BstMBI GATC 7 cut(s) 4, 132, 237, 638, 784, 1087, 1107
BstMCI CGRYCG 2 cut(s) 141, 1090
BstMWI GCNNNNNNNGC 5 cut(s) 55, 365, 401, 1028, 1042
BstNI CCWGG 1 cut(s) 490
BstNSI RCATGY 1 cut(s) 715
BstPAI GACNNNNGTC 1 cut(s) 306
BstSCI CCNGG 5 cut(s) 466, 467, 488, 773, 1031
BstSFI CTRYAG 1 cut(s) 1017
BstSLI GKGCMC 1 cut(s) 1149
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 9 cut(s) 48, 70, 156, 346, 379, 726, 882, 1003, 1031
BstV2I GAAGAC 1 cut(s) 693
BstX2I RGATCY 2 cut(s) 4, 638
BstYI RGATCY 2 cut(s) 4, 638
BstZI CGGCCG 1 cut(s) 138
BsuRI GGCC 6 cut(s) 140, 267, 404, 444, 605, 1085
BtgZI GCGATG 1 cut(s) 709
BtsCI GGATG 1 cut(s) 111
BtsI GCAGTG 1 cut(s) 819
BtsIMutI CAGTG 1 cut(s) 819
BveI ACCTGC 1 cut(s) 325
Cac8I GCNNGC 3 cut(s) 80, 142, 744
CaiI CAGNNNCTG 2 cut(s) 172, 1016
CciI TCATGA 1 cut(s) 609
Cfr10I RCCGGY 2 cut(s) 103, 140
Cfr13I GGNCC 4 cut(s) 152, 163, 252, 266
Cfr9I CCCGGG 1 cut(s) 467
CseI GACGC 2 cut(s) 7, 1040
Csp6I GTAC 4 cut(s) 97, 458, 958, 1151
CviAII CATG 7 cut(s) 235, 355, 420, 566, 610, 706, 712
CviQI GTAC 4 cut(s) 97, 458, 958, 1151
DdeI CTNAG 5 cut(s) 8, 159, 655, 661, 768
DpnI GATC 7 cut(s) 6, 134, 239, 640, 786, 1089, 1109
DpnII GATC 7 cut(s) 4, 132, 237, 638, 784, 1087, 1107
EaeI YGGCCR 2 cut(s) 138, 1083
EagI CGGCCG 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 1106
EarI CTCTTC 1 cut(s) 1106
EciI GGCGGA 1 cut(s) 924
Ecl136II GAGCTC 1 cut(s) 226
EclXI CGGCCG 1 cut(s) 138
Eco130I CCWWGG 1 cut(s) 1064
Eco147I AGGCCT 1 cut(s) 444
Eco24I GRGCYC 1 cut(s) 228
Eco47I GGWCC 3 cut(s) 152, 163, 252
Eco52I CGGCCG 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 2 cut(s) 50, 338
Eco72I CACGTG 1 cut(s) 118
Eco88I CYCGRG 2 cut(s) 406, 467
EcoICRI GAGCTC 1 cut(s) 226
EcoNI CCTNNNNNAGG 1 cut(s) 449
EcoO109I RGGNCCY 1 cut(s) 252
EcoRI GAATTC 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 488
EcoT14I CCWWGG 1 cut(s) 1064
EcoT22I ATGCAT 1 cut(s) 550
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 1064
Esp3I CGTCTC 1 cut(s) 428
FaeI CATG 7 cut(s) 238, 358, 423, 569, 613, 709, 715
FalI AAGNNNNNCTT 2 cut(s) 590, 622
FaqI GGGAC 1 cut(s) 238
FatI CATG 7 cut(s) 234, 354, 419, 565, 609, 705, 711
FbaI TGATCA 1 cut(s) 1107
FokI GGATG 1 cut(s) 98
FriOI GRGCYC 1 cut(s) 228
FspBI CTAG 1 cut(s) 258
GsaI CCCAGC 1 cut(s) 274
HaeIII GGCC 6 cut(s) 140, 267, 404, 444, 605, 1085
HapII CCGG 7 cut(s) 104, 141, 468, 774, 966, 1032, 1125
HgaI GACGC 2 cut(s) 7, 1040
Hin1II CATG 7 cut(s) 238, 358, 423, 569, 613, 709, 715
HincII GTYRAC 2 cut(s) 478, 1075
HindII GTYRAC 2 cut(s) 478, 1075
HindIII AAGCTT 1 cut(s) 861
HinfI GANTC 4 cut(s) 157, 770, 829, 935
HpaII CCGG 7 cut(s) 104, 141, 468, 774, 966, 1032, 1125
HphI GGTGA 2 cut(s) 695, 830
Hpy166II GTNNAC 8 cut(s) 97, 195, 301, 343, 478, 934, 997, 1075
Hpy188I TCNGA 7 cut(s) 9, 69, 156, 247, 318, 384, 769
Hpy188III TCNNGA 6 cut(s) 161, 408, 581, 610, 826, 1105
Hpy8I GTNNAC 8 cut(s) 97, 195, 301, 343, 478, 934, 997, 1075
HpyAV CCTTC 7 cut(s) 68, 223, 333, 587, 616, 652, 998
HpyCH4III ACNGT 3 cut(s) 595, 1072, 1132
HpyCH4IV ACGT 3 cut(s) 117, 960, 1149
HpyCH4V TGCA 6 cut(s) 503, 548, 709, 812, 1019, 1166
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 365, 401, 1028, 1042
HpyF3I CTNAG 5 cut(s) 8, 159, 655, 661, 768
HpySE526I ACGT 3 cut(s) 117, 960, 1149
Hsp92II CATG 7 cut(s) 238, 358, 423, 569, 613, 709, 715
KflI GGGWCCC 1 cut(s) 252
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
Ksp22I TGATCA 1 cut(s) 1107
Kzo9I GATC 7 cut(s) 4, 132, 237, 638, 784, 1087, 1107
LmnI GCTCC 2 cut(s) 524, 588
Lsp1109I GCAGC 9 cut(s) 48, 70, 156, 346, 379, 726, 882, 1003, 1031
LweI GCATC 2 cut(s) 535, 557
MaeI CTAG 1 cut(s) 258
MaeII ACGT 3 cut(s) 117, 960, 1149
MalI GATC 7 cut(s) 6, 134, 239, 640, 786, 1089, 1109
MboI GATC 7 cut(s) 4, 132, 237, 638, 784, 1087, 1107
MboII GAAGA 7 cut(s) 232, 634, 698, 785, 1013, 1093, 1127
MflI RGATCY 2 cut(s) 4, 638
MhlI GDGCHC 3 cut(s) 228, 593, 1149
MluCI AATT 3 cut(s) 415, 806, 1118
MlyI GAGTC 3 cut(s) 151, 779, 929
MmeI TCCRAC 1 cut(s) 179
Mph1103I ATGCAT 1 cut(s) 550
MroNI GCCGGC 1 cut(s) 140
MseI TTAA 4 cut(s) 330, 597, 840, 876
MslI CAYNNNNRTG 3 cut(s) 116, 710, 992
MspA1I CMGCKG 4 cut(s) 61, 169, 274, 1022
MspI CCGG 7 cut(s) 104, 141, 468, 774, 966, 1032, 1125
MspR9I CCNGG 5 cut(s) 468, 469, 490, 775, 1033
MvaI CCWGG 1 cut(s) 490
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 5 cut(s) 55, 365, 401, 1028, 1042
NaeI GCCGGC 1 cut(s) 142
NciI CCSGG 4 cut(s) 468, 469, 775, 1033
NdeII GATC 7 cut(s) 4, 132, 237, 638, 784, 1087, 1107
NgoMIV GCCGGC 1 cut(s) 140
NlaIII CATG 7 cut(s) 238, 358, 423, 569, 613, 709, 715
NlaIV GGNNCC 4 cut(s) 153, 253, 254, 268
NmeAIII GCCGAG 1 cut(s) 247
NsiI ATGCAT 1 cut(s) 550
NspI RCATGY 1 cut(s) 715
PagI TCATGA 1 cut(s) 609
PaqCI CACCTGC 1 cut(s) 325
PceI AGGCCT 1 cut(s) 444
PciI ACATGT 1 cut(s) 711
PcsI WCGNNNNNNNCGW 1 cut(s) 996
PdiI GCCGGC 1 cut(s) 142
PfeI GAWTC 1 cut(s) 829
PflMI CCANNNNNTGG 1 cut(s) 905
Ple19I CGATCG 1 cut(s) 1090
PleI GAGTC 3 cut(s) 151, 778, 929
PmaCI CACGTG 1 cut(s) 118
PmlI CACGTG 1 cut(s) 118
PpsI GAGTC 3 cut(s) 151, 778, 929
Ppu21I YACGTR 2 cut(s) 118, 1150
PpuMI RGGWCCY 1 cut(s) 252
PscI ACATGT 1 cut(s) 711
PshAI GACNNNNGTC 1 cut(s) 306
Psp124BI GAGCTC 1 cut(s) 228
Psp5II RGGWCCY 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 488
PspCI CACGTG 1 cut(s) 118
PspFI CCCAGC 1 cut(s) 270
PspGI CCWGG 1 cut(s) 488
PspN4I GGNNCC 4 cut(s) 153, 253, 254, 268
PspPI GGNCC 4 cut(s) 152, 163, 252, 266
PspPPI RGGWCCY 1 cut(s) 252
PsrI GAACNNNNNNTAC 2 cut(s) 80, 112
PstI CTGCAG 1 cut(s) 1021
PstNI CAGNNNCTG 2 cut(s) 172, 1016
PsuI RGATCY 2 cut(s) 4, 638
PvuI CGATCG 1 cut(s) 1090
PvuII CAGCTG 4 cut(s) 61, 169, 274, 1022
RsaI GTAC 4 cut(s) 98, 459, 959, 1152
RsaNI GTAC 4 cut(s) 97, 458, 958, 1151
RseI CAYNNNNRTG 3 cut(s) 116, 710, 992
SacI GAGCTC 1 cut(s) 228
SaqAI TTAA 4 cut(s) 330, 597, 840, 876
Sau3AI GATC 7 cut(s) 4, 132, 237, 638, 784, 1087, 1107
Sau96I GGNCC 4 cut(s) 152, 163, 252, 266
SchI GAGTC 3 cut(s) 151, 779, 929
ScrFI CCNGG 5 cut(s) 468, 469, 490, 775, 1033
SduI GDGCHC 3 cut(s) 228, 593, 1149
SfaNI GCATC 2 cut(s) 535, 557
SfcI CTRYAG 1 cut(s) 1017
SgrAI CRCCGGYG 1 cut(s) 103
SinI GGWCC 3 cut(s) 152, 163, 252
SmaI CCCGGG 1 cut(s) 469
SmiMI CAYNNNNRTG 3 cut(s) 116, 710, 992
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
Sse9I AATT 3 cut(s) 415, 806, 1118
SseBI AGGCCT 1 cut(s) 444
SsiI CCGC 5 cut(s) 137, 219, 261, 264, 909
SspMI CTAG 1 cut(s) 258
SstI GAGCTC 1 cut(s) 228
StuI AGGCCT 1 cut(s) 444
StyD4I CCNGG 5 cut(s) 466, 467, 488, 773, 1031
StyI CCWWGG 1 cut(s) 1064
TaaI ACNGT 3 cut(s) 595, 1072, 1132
TaiI ACGT 3 cut(s) 120, 963, 1152
TaqI TCGA 4 cut(s) 86, 375, 582, 990
TaqII GACCGA 1 cut(s) 86
TasI AATT 3 cut(s) 415, 806, 1118
TatI WGTACW 1 cut(s) 96
TauI GCSGC 4 cut(s) 140, 221, 264, 267
TfiI GAWTC 1 cut(s) 829
Tru1I TTAA 4 cut(s) 330, 597, 840, 876
Tru9I TTAA 4 cut(s) 330, 597, 840, 876
TscAI CASTG 1 cut(s) 819
TseI GCWGC 9 cut(s) 58, 61, 169, 359, 392, 739, 895, 1016, 1019
TspDTI ATGAA 6 cut(s) 408, 598, 659, 837, 976, 1129
TspMI CCCGGG 1 cut(s) 467
TspRI CASTG 1 cut(s) 819
Van91I CCANNNNNTGG 1 cut(s) 905
VpaK11BI GGWCC 3 cut(s) 152, 163, 252
XagI CCTNNNNNAGG 1 cut(s) 449
XapI RAATTY 2 cut(s) 415, 806
XceI RCATGY 1 cut(s) 715
XmaI CCCGGG 1 cut(s) 467
XspI CTAG 1 cut(s) 258
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.