pycom08g03320

Belongs to the serpin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002697
Physical Location & Seq
Forward (+)
2572286 .. 2574334
2049 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03320.1

Sequence Viewer

Length: 1167 bp
ATGGATCTGAGAGAAAGCATCATCAACCAAAACGACTACGTTCTGGAGCTGACGAAGCAGCTGCTTCAGACCGAAGGCAAGCAATCGAACCTCGTGTACTCGCCGCTGTCCATCCACGTGCTGTTGAGCCTGATCGCGGCCGGGTCAAAGGGTCCGACTCAGGACCAGTTGCTGTCTTTTCTCAAGTCAAAGTCCACCGACCACCTCAACTCCTTCGCCGCCGAGCTCGTCTCTGTGATCTTCTCCGATGGGTCCCCTAGCGGCGGCCCCCAGCTGTCGTTTGCGAATGGTGGTTGGGTGGACAGGCGTCTCCCTCTGAAGCCATCTTTCACGCAGGTGGTGGAAACTTCTTACACGGCCGCTCTCGCTCAAGTCGATTTTCAGACCAATGCTGCTCAAGTGGCCTTGGGAGTGAATTCATGGGCTGAAAGGGAGACGAGAGGCCTTATCAATGAGGTACTTCCTCCCGAGTCAGTTGACTGCTCAACCAAGCTCATCTTTGCAAATGCGTTATACTTCAAAGGAGCTTGGACTGAAAAGTTTGATGCATCACAAACGAAAGAGCATGACTTCCACCTTCTCGATGGAAGCACAGTTAAGGTGCCCTTCATGACCAGCAGGAAGAAGCAGTATGTAAGATCCTTCAATGGTTTCTCAGTCTTAGGGCTTCATTACAAGCAAGGTGAAGACAAGCGATGTTTCTCCATGCACATTTTTCTTCCAGAGGCGAAAGATGGGCTGCCAGCTTTGGTTGAGAAACTTGGTTCTGAGTCCGGGTTTTTGGATCGCCATCTTCCCAAGCAAAAATTTGCAGTGGGTGATTTCAGGATTCCAAAGTTTAAGATTTCATTTGGATTTGAAGCTTCGAATGTCCTTAAAGGTCTGGGCTTGGTGCTGCCATTCGGTGGTGGAGGAGGTTTGACTGAGATGGTGGACTCGCCTGAGGGTAAGAACTCATACGTTTCCCGCATATTCCATAAGTCGTTCATCGAAGTGAACGAAGAAGGCACGGAAGCCGCAGCTGCTTCCGCCGTTGTTAAAACTATGATGTGTCTGCCGAGGACGGTTGACTTTGTGGCTGATCGTCCATTCCTCTTCTTGGTCAAGGAAGAATCGACCGGAGCCGTGCTGTTCATCGGCCATGTACTCAATCCTCTTGCAGGCTGA

Protein Analysis

389

Amino Acids

42.48

Weight (kDa)

6.33

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 10 - 385 1.8e-95 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 325
Acc36I ACCTGC 1 cut(s) 325
AccB1I GGYRCC 1 cut(s) 601
AccB7I CCANNNNNTGG 1 cut(s) 905
AccBSI CCGCTC 1 cut(s) 362
AccII CGCG 1 cut(s) 137
AciI CCGC 9 cut(s) 104, 137, 219, 261, 264, 360, 967, 1017, 1029
AclWI GGATC 3 cut(s) 12, 633, 792
AcoI YGGCCR 3 cut(s) 138, 357, 1138
AcsI RAATTY 2 cut(s) 415, 806
AcuI CTGAAG 2 cut(s) 50, 338
AcvI CACGTG 1 cut(s) 118
AcyI GRCGYC 1 cut(s) 307
AfaI GTAC 3 cut(s) 98, 459, 1146
AfiI CCNNNNNNNGG 5 cut(s) 136, 263, 905, 1099, 1160
AgsI TTSAA 3 cut(s) 520, 646, 860
AleI CACNNNNGTG 1 cut(s) 335
AluBI AGCT 9 cut(s) 49, 61, 226, 274, 493, 527, 746, 863, 1022
AluI AGCT 9 cut(s) 49, 61, 226, 274, 493, 527, 746, 863, 1022
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 3 cut(s) 235, 314, 428
AlwI GGATC 3 cut(s) 12, 633, 792
AlwNI CAGNNNCTG 1 cut(s) 172
Ama87I CYCGRG 1 cut(s) 467
AoxI GGCC 6 cut(s) 138, 265, 357, 402, 442, 1138
ApeKI GCWGC 7 cut(s) 58, 61, 392, 739, 895, 1019, 1022
ApoI RAATTY 2 cut(s) 415, 806
AspS9I GGNCC 4 cut(s) 152, 163, 252, 266
AsuC2I CCSGG 2 cut(s) 142, 775
AsuHPI GGTGA 2 cut(s) 695, 830
AsuII TTCGAA 1 cut(s) 866
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 3 cut(s) 152, 163, 252
AxyI CCTNAGG 1 cut(s) 942
BaeGI GKGCMC 1 cut(s) 606
BanI GGYRCC 1 cut(s) 601
BanII GRGCYC 1 cut(s) 228
BauI CACGAG 1 cut(s) 92
BbrPI CACGTG 1 cut(s) 118
BbsI GAAGAC 1 cut(s) 693
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 7 cut(s) 48, 70, 379, 726, 882, 1009, 1031
BccI CCATC 7 cut(s) 119, 242, 331, 578, 728, 798, 922
BceAI ACGGC 3 cut(s) 372, 1016, 1109
BcgI CGANNNNNNTGC 2 cut(s) 43, 77
BcnI CCSGG 2 cut(s) 142, 775
BcoDI GTCTC 3 cut(s) 235, 314, 428
BfaI CTAG 1 cut(s) 258
BfuAI ACCTGC 1 cut(s) 325
Bme1390I CCNGG 2 cut(s) 142, 775
Bme18I GGWCC 3 cut(s) 152, 163, 252
BmeT110I CYCGRG 1 cut(s) 467
BmgT120I GGNCC 4 cut(s) 152, 163, 252, 266
BmiI GGNNCC 6 cut(s) 153, 253, 254, 268, 603, 1123
BmrFI CCNGG 2 cut(s) 142, 775
BmsI GCATC 3 cut(s) 27, 535, 557
BoxI GACNNNNGTC 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 693
BplI GAGNNNNNCTC 2 cut(s) 215, 247
BpmI CTGGAG 1 cut(s) 65
Bpu14I TTCGAA 1 cut(s) 866
BpuEI CTTGAG 3 cut(s) 167, 354, 381
BpuMI CCSGG 2 cut(s) 142, 775
BsaAI YACGTR 1 cut(s) 118
BsaBI GATNNNNATC 1 cut(s) 789
BsaHI GRCGYC 1 cut(s) 307
BsaJI CCNNGG 2 cut(s) 405, 1058
BsaWI WCCGGW 1 cut(s) 1118
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 5 cut(s) 136, 263, 905, 1099, 1160
Bse1I ACTGG 1 cut(s) 166
Bse21I CCTNAGG 1 cut(s) 942
Bse8I GATNNNNATC 1 cut(s) 789
BseDI CCNNGG 2 cut(s) 405, 1058
BseGI GGATG 1 cut(s) 111
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 5 cut(s) 136, 263, 905, 1099, 1160
BseMII CTCAG 5 cut(s) 173, 669, 759, 915, 933
BseNI ACTGG 1 cut(s) 166
BseRI GAGGAG 1 cut(s) 927
BseSI GKGCMC 1 cut(s) 606
BseX3I CGGCCG 2 cut(s) 138, 357
BseXI GCAGC 7 cut(s) 48, 70, 379, 726, 882, 1009, 1031
BseYI CCCAGC 1 cut(s) 270
Bsh1236I CGCG 1 cut(s) 137
Bsh1285I CGRYCG 3 cut(s) 141, 360, 1119
BshFI GGCC 6 cut(s) 140, 267, 359, 404, 444, 1140
BshNI GGYRCC 1 cut(s) 601
BsiEI CGRYCG 3 cut(s) 141, 360, 1119
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 467
BsiSI CCGG 3 cut(s) 141, 774, 1119
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 5 cut(s) 136, 263, 905, 1099, 1160
BsmAI GTCTC 3 cut(s) 235, 314, 428
BsmBI CGTCTC 3 cut(s) 235, 314, 428
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 6 cut(s) 140, 267, 359, 404, 444, 1140
BsoBI CYCGRG 1 cut(s) 467
Bsp119I TTCGAA 1 cut(s) 866
Bsp1286I GDGCHC 2 cut(s) 228, 606
Bsp143I GATC 6 cut(s) 4, 132, 237, 638, 784, 1081
BspACI CCGC 9 cut(s) 104, 137, 219, 261, 264, 360, 967, 1017, 1029
BspANI GGCC 6 cut(s) 140, 267, 359, 404, 444, 1140
BspCNI CTCAG 5 cut(s) 172, 668, 760, 916, 934
BspFNI CGCG 1 cut(s) 137
BspHI TCATGA 1 cut(s) 609
BspLI GGNNCC 6 cut(s) 153, 253, 254, 268, 603, 1123
BspMI ACCTGC 1 cut(s) 325
BspPI GGATC 3 cut(s) 12, 633, 792
BspT104I TTCGAA 1 cut(s) 866
BspT107I GGYRCC 1 cut(s) 601
BsrBI CCGCTC 1 cut(s) 362
BsrI ACTGG 1 cut(s) 166
BssECI CCNNGG 2 cut(s) 405, 1058
BssMI GATC 6 cut(s) 4, 132, 237, 638, 784, 1081
BssNI GRCGYC 1 cut(s) 307
BssSI CACGAG 1 cut(s) 92
BssT1I CCWWGG 1 cut(s) 405
Bst2BI CACGAG 1 cut(s) 92
Bst4CI ACNGT 2 cut(s) 595, 1066
Bst6I CTCTTC 1 cut(s) 1100
BstACI GRCGYC 1 cut(s) 307
BstBAI YACGTR 1 cut(s) 118
BstBI TTCGAA 1 cut(s) 866
BstC8I GCNNGC 3 cut(s) 80, 744, 1162
BstDEI CTNAG 7 cut(s) 8, 159, 655, 661, 768, 924, 942
BstENI CCTNNNNNAGG 1 cut(s) 1158
BstF5I GGATG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 137
BstKTI GATC 6 cut(s) 7, 135, 240, 641, 787, 1084
BstMAI GTCTC 3 cut(s) 235, 314, 428
BstMBI GATC 6 cut(s) 4, 132, 237, 638, 784, 1081
BstMCI CGRYCG 3 cut(s) 141, 360, 1119
BstMWI GCNNNNNNNGC 5 cut(s) 55, 365, 401, 1022, 1028
BstPAI GACNNNNGTC 1 cut(s) 306
BstSCI CCNGG 2 cut(s) 140, 773
BstSLI GKGCMC 1 cut(s) 606
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 7 cut(s) 48, 70, 379, 726, 882, 1009, 1031
BstV2I GAAGAC 1 cut(s) 693
BstX2I RGATCY 2 cut(s) 4, 638
BstYI RGATCY 2 cut(s) 4, 638
BstZI CGGCCG 2 cut(s) 138, 357
Bsu36I CCTNAGG 1 cut(s) 942
BsuRI GGCC 6 cut(s) 140, 267, 359, 404, 444, 1140
BtgZI GCGATG 1 cut(s) 709
BtsCI GGATG 1 cut(s) 111
BtsI GCAGTG 1 cut(s) 819
BtsIMutI CAGTG 1 cut(s) 819
BveI ACCTGC 1 cut(s) 325
Cac8I GCNNGC 3 cut(s) 80, 744, 1162
CaiI CAGNNNCTG 1 cut(s) 172
CciI TCATGA 1 cut(s) 609
Cfr13I GGNCC 4 cut(s) 152, 163, 252, 266
CseI GACGC 1 cut(s) 296
Csp6I GTAC 3 cut(s) 97, 458, 1145
CviAII CATG 5 cut(s) 420, 566, 610, 706, 1142
CviQI GTAC 3 cut(s) 97, 458, 1145
DdeI CTNAG 7 cut(s) 8, 159, 655, 661, 768, 924, 942
DpnI GATC 6 cut(s) 6, 134, 239, 640, 786, 1083
DpnII GATC 6 cut(s) 4, 132, 237, 638, 784, 1081
EaeI YGGCCR 3 cut(s) 138, 357, 1138
EagI CGGCCG 2 cut(s) 138, 357
Eam1104I CTCTTC 1 cut(s) 1100
EarI CTCTTC 1 cut(s) 1100
EciI GGCGGA 1 cut(s) 1018
Ecl136II GAGCTC 1 cut(s) 226
EclXI CGGCCG 2 cut(s) 138, 357
Eco130I CCWWGG 1 cut(s) 405
Eco147I AGGCCT 1 cut(s) 444
Eco24I GRGCYC 1 cut(s) 228
Eco47I GGWCC 3 cut(s) 152, 163, 252
Eco52I CGGCCG 2 cut(s) 138, 357
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 2 cut(s) 50, 338
Eco72I CACGTG 1 cut(s) 118
Eco81I CCTNAGG 1 cut(s) 942
Eco88I CYCGRG 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 226
EcoNI CCTNNNNNAGG 1 cut(s) 1158
EcoO109I RGGNCCY 1 cut(s) 252
EcoRI GAATTC 1 cut(s) 415
EcoT14I CCWWGG 1 cut(s) 405
EcoT22I ATGCAT 1 cut(s) 550
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 405
Esp3I CGTCTC 3 cut(s) 235, 314, 428
FaeI CATG 5 cut(s) 423, 569, 613, 709, 1145
FalI AAGNNNNNCTT 4 cut(s) 482, 514, 590, 622
FaqI GGGAC 1 cut(s) 238
FatI CATG 5 cut(s) 419, 565, 609, 705, 1141
FauI CCCGC 1 cut(s) 974
FokI GGATG 1 cut(s) 98
FriOI GRGCYC 1 cut(s) 228
FspBI CTAG 1 cut(s) 258
GsaI CCCAGC 1 cut(s) 274
GsuI CTGGAG 1 cut(s) 65
HaeIII GGCC 6 cut(s) 140, 267, 359, 404, 444, 1140
HapII CCGG 3 cut(s) 141, 774, 1119
HgaI GACGC 1 cut(s) 296
Hin1I GRCGYC 1 cut(s) 307
Hin1II CATG 5 cut(s) 423, 569, 613, 709, 1145
HincII GTYRAC 2 cut(s) 478, 1069
HindII GTYRAC 2 cut(s) 478, 1069
HindIII AAGCTT 1 cut(s) 861
HinfI GANTC 6 cut(s) 157, 470, 770, 829, 935, 1112
HpaII CCGG 3 cut(s) 141, 774, 1119
HphI GGTGA 2 cut(s) 695, 830
Hpy166II GTNNAC 7 cut(s) 97, 195, 301, 478, 934, 997, 1069
Hpy188I TCNGA 7 cut(s) 9, 69, 156, 247, 318, 384, 769
Hpy188III TCNNGA 7 cut(s) 44, 161, 467, 581, 610, 722, 826
Hpy8I GTNNAC 7 cut(s) 97, 195, 301, 478, 934, 997, 1069
HpyAV CCTTC 6 cut(s) 68, 223, 587, 616, 652, 998
HpyCH4III ACNGT 2 cut(s) 595, 1066
HpyCH4IV ACGT 3 cut(s) 39, 117, 960
HpyCH4V TGCA 5 cut(s) 503, 548, 709, 812, 1160
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 365, 401, 1022, 1028
HpyF3I CTNAG 7 cut(s) 8, 159, 655, 661, 768, 924, 942
HpySE526I ACGT 3 cut(s) 39, 117, 960
Hsp92I GRCGYC 1 cut(s) 307
Hsp92II CATG 5 cut(s) 423, 569, 613, 709, 1145
KflI GGGWCCC 1 cut(s) 252
Kzo9I GATC 6 cut(s) 4, 132, 237, 638, 784, 1081
LmnI GCTCC 3 cut(s) 46, 524, 1121
Lsp1109I GCAGC 7 cut(s) 48, 70, 379, 726, 882, 1009, 1031
LweI GCATC 3 cut(s) 27, 535, 557
MaeI CTAG 1 cut(s) 258
MaeII ACGT 3 cut(s) 39, 117, 960
MalI GATC 6 cut(s) 6, 134, 239, 640, 786, 1083
MbiI CCGCTC 1 cut(s) 362
MboI GATC 6 cut(s) 4, 132, 237, 638, 784, 1081
MboII GAAGA 8 cut(s) 232, 634, 698, 710, 785, 1013, 1087, 1121
MflI RGATCY 2 cut(s) 4, 638
MhlI GDGCHC 2 cut(s) 228, 606
MluCI AATT 2 cut(s) 415, 806
MlyI GAGTC 4 cut(s) 151, 479, 779, 929
MmeI TCCRAC 1 cut(s) 179
Mph1103I ATGCAT 1 cut(s) 550
MseI TTAA 4 cut(s) 597, 840, 876, 1038
MslI CAYNNNNRTG 3 cut(s) 116, 335, 992
MspA1I CMGCKG 4 cut(s) 61, 106, 274, 1022
MspI CCGG 3 cut(s) 141, 774, 1119
MspR9I CCNGG 2 cut(s) 142, 775
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 5 cut(s) 55, 365, 401, 1022, 1028
NciI CCSGG 2 cut(s) 142, 775
NdeII GATC 6 cut(s) 4, 132, 237, 638, 784, 1081
NlaIII CATG 5 cut(s) 423, 569, 613, 709, 1145
NlaIV GGNNCC 6 cut(s) 153, 253, 254, 268, 603, 1123
NmeAIII GCCGAG 2 cut(s) 247, 1083
NsiI ATGCAT 1 cut(s) 550
NspV TTCGAA 1 cut(s) 866
OliI CACNNNNGTG 1 cut(s) 335
PagI TCATGA 1 cut(s) 609
PaqCI CACCTGC 1 cut(s) 325
PceI AGGCCT 1 cut(s) 444
PcsI WCGNNNNNNNCGW 2 cut(s) 372, 996
PfeI GAWTC 2 cut(s) 829, 1112
PflMI CCANNNNNTGG 1 cut(s) 905
PleI GAGTC 4 cut(s) 151, 478, 778, 929
PmaCI CACGTG 1 cut(s) 118
PmlI CACGTG 1 cut(s) 118
PpsI GAGTC 4 cut(s) 151, 478, 778, 929
Ppu21I YACGTR 1 cut(s) 118
PpuMI RGGWCCY 1 cut(s) 252
PshAI GACNNNNGTC 1 cut(s) 306
Psp124BI GAGCTC 1 cut(s) 228
Psp5II RGGWCCY 1 cut(s) 252
PspCI CACGTG 1 cut(s) 118
PspFI CCCAGC 1 cut(s) 270
PspN4I GGNNCC 6 cut(s) 153, 253, 254, 268, 603, 1123
PspPI GGNCC 4 cut(s) 152, 163, 252, 266
PspPPI RGGWCCY 1 cut(s) 252
PsrI GAACNNNNNNTAC 2 cut(s) 80, 112
PstNI CAGNNNCTG 1 cut(s) 172
PsuI RGATCY 2 cut(s) 4, 638
PvuII CAGCTG 3 cut(s) 61, 274, 1022
RsaI GTAC 3 cut(s) 98, 459, 1146
RsaNI GTAC 3 cut(s) 97, 458, 1145
RseI CAYNNNNRTG 3 cut(s) 116, 335, 992
SacI GAGCTC 1 cut(s) 228
SaqAI TTAA 4 cut(s) 597, 840, 876, 1038
Sau3AI GATC 6 cut(s) 4, 132, 237, 638, 784, 1081
Sau96I GGNCC 4 cut(s) 152, 163, 252, 266
SchI GAGTC 4 cut(s) 151, 479, 779, 929
ScrFI CCNGG 2 cut(s) 142, 775
SduI GDGCHC 2 cut(s) 228, 606
SfaNI GCATC 3 cut(s) 27, 535, 557
SfuI TTCGAA 1 cut(s) 866
SinI GGWCC 3 cut(s) 152, 163, 252
SmiMI CAYNNNNRTG 3 cut(s) 116, 335, 992
SmlI CTYRAG 3 cut(s) 182, 369, 396
SmoI CTYRAG 3 cut(s) 182, 369, 396
Sse9I AATT 2 cut(s) 415, 806
SseBI AGGCCT 1 cut(s) 444
SsiI CCGC 9 cut(s) 104, 137, 219, 261, 264, 360, 967, 1017, 1029
SspMI CTAG 1 cut(s) 258
SstI GAGCTC 1 cut(s) 228
StuI AGGCCT 1 cut(s) 444
StyD4I CCNGG 2 cut(s) 140, 773
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 2 cut(s) 595, 1066
TaiI ACGT 3 cut(s) 42, 120, 963
TaqI TCGA 6 cut(s) 86, 375, 582, 866, 990, 1115
TaqII GACCGA 1 cut(s) 86
TasI AATT 2 cut(s) 415, 806
TatI WGTACW 2 cut(s) 96, 1144
TauI GCSGC 7 cut(s) 106, 140, 221, 264, 267, 362, 1019
TfiI GAWTC 2 cut(s) 829, 1112
Tru1I TTAA 4 cut(s) 597, 840, 876, 1038
Tru9I TTAA 4 cut(s) 597, 840, 876, 1038
TscAI CASTG 1 cut(s) 819
TseI GCWGC 7 cut(s) 58, 61, 392, 739, 895, 1019, 1022
TspDTI ATGAA 6 cut(s) 408, 598, 659, 837, 976, 1123
TspGWI ACGGA 1 cut(s) 1025
TspRI CASTG 1 cut(s) 819
Van91I CCANNNNNTGG 1 cut(s) 905
VpaK11BI GGWCC 3 cut(s) 152, 163, 252
XagI CCTNNNNNAGG 1 cut(s) 1158
XapI RAATTY 2 cut(s) 415, 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 581
XspI CTAG 1 cut(s) 258
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.