MD08G1041600.v1.1

Belongs to the serpin family

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
Physical Location & Seq
Forward (+)
3063406 .. 3067974
4569 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1041600.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGGATCTGAGAGAAAGCATCATCAACCAAAACGACGTCGCAAGGAGGCTGACGAAGCAGCTGGTTCAGACCGAAGGCAAGCAGTTGAACCTCGTGTACTCGCCGCTGTCCATCCACGTGGTGCTGAGCCTGATCACAGCCGGGTCAAAGGGTCCCACCCAGGAGCAGCTGCTCTCATTCCTCAAGTCAAAGTCCACCGATCACCTCAACTCCTTCGCCTCCCAGCTAGTGTCCGTGATCTTCTCCGATGGGTCCCCCGTGGGCGGCCCCAAGCTGGCGTTTGCGAATGGGCTTTGGGTGCACAAGCCTCTCACGCTGAAGCCTTCCTTCAAGCAGGTGGTGGACGCTTCTTACAAGGCAGCTCTCGCCCTAGTCGATTTTCAGACTAATACTGAGGCTGCTCAAGTGGCGTCGGGAGTGAATTCATGGGCTGAAAAGGAGACGAGCGGCCTTATCAAGGATCTACTTCCTCCCAGGTCACTTGACAGCTCAACCAGGCTCATCTTTACGAATGCATTGTACTTCAAAGGAGCTTGGACTGAGAAATTTGATGCATCAGCTACAAAAGAGCATGACTTCCACCTTCTCGACGGGAGCACAGTTAAGGCGCCCTTCATGACCAGCAAGAGGAAGCAGTATGTAAGTGCCTTTGACGGTTTCTCAGTCTTAGGGCTTCCTTACAAACAAGTTGAAGACAAGCGGCGCTTCTCCGTGCACATCTTTCTTCCAGAGGAGAAAGATGGGCTGCCAGCTTTGGTTGAGAAACTTGGCTCTGAGTCCGGGTTTTTGGATCGATATCTTCCCAAACAACCAGTTGCAGTGGGCGACTTCAGAATCCCAAAGTTTAAGATCTCATTTGGGTTTGAAGCTTCCAATGTCCTAAAGAGTCTGGGACTGGTGCTGCCTTTCGAGGGTGAAGGAGGTCTGACAGAGATGGTGGACTCGCCCGAGGGTAAGAAGCTATATGTTTCCAGCATATTTCACAAGTCGTTCATCGAAGTGAATGAAGAAGGCACAGAAGCTGCAGCTGCTTCTGCCGGCGTTATAAGTCAGAGGAGTATGCCCATGACATTTGTGGCTGATCATCCATTCCTCTTCACGATCAGGGAAGAAACCACAGGAACAGTTCTGTTCATCGGTAACCTGCTCAACCCTCTTGCCGGCTGA

Protein Analysis

389

Amino Acids

42.47

Weight (kDa)

6.73

Isoelectric Point (pI)

38.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 9 - 385 4.4e-98 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1046
AarI CACCTGC 1 cut(s) 325
AatII GACGTC 1 cut(s) 39
Acc36I ACCTGC 2 cut(s) 325, 1152
AccB1I GGYRCC 1 cut(s) 607
AccBSI CCGCTC 1 cut(s) 447
AciI CCGC 4 cut(s) 104, 264, 447, 700
AclWI GGATC 3 cut(s) 12, 468, 798
AcsI RAATTY 2 cut(s) 421, 545
AcuI CTGAAG 2 cut(s) 338, 814
AcvI CACGTG 1 cut(s) 118
AcyI GRCGYC 3 cut(s) 36, 410, 608
AdeI CACNNNGTG 1 cut(s) 121
AfaI GTAC 2 cut(s) 98, 521
AfiI CCNNNNNNNGG 6 cut(s) 263, 274, 457, 627, 911, 1160
AgsI TTSAA 5 cut(s) 88, 331, 526, 692, 866
AjnI CCWGG 3 cut(s) 159, 473, 494
Alw21I GWGCWC 3 cut(s) 303, 599, 717
Alw26I GTCTC 1 cut(s) 434
Alw44I GTGCAC 2 cut(s) 299, 713
AlwI GGATC 3 cut(s) 12, 468, 798
AlwNI CAGNNNCTG 1 cut(s) 1022
Ama87I CYCGRG 1 cut(s) 947
AoxI GGCC 2 cut(s) 265, 448
ApaLI GTGCAC 2 cut(s) 299, 713
ApoI RAATTY 2 cut(s) 421, 545
AspLEI GCGC 2 cut(s) 610, 705
AspS9I GGNCC 3 cut(s) 152, 252, 266
AsuC2I CCSGG 2 cut(s) 142, 781
AsuHPI GGTGA 2 cut(s) 194, 926
AvaI CYCGRG 1 cut(s) 947
AvaII GGWCC 2 cut(s) 152, 252
BaeGI GKGCMC 2 cut(s) 303, 717
BanI GGYRCC 1 cut(s) 607
BauI CACGAG 1 cut(s) 92
BbrPI CACGTG 1 cut(s) 118
BbsI GAAGAC 1 cut(s) 699
Bbv12I GWGCWC 3 cut(s) 303, 599, 717
BccI CCATC 4 cut(s) 119, 242, 734, 928
BciT130I CCWGG 3 cut(s) 161, 475, 496
BclI TGATCA 2 cut(s) 132, 1081
BcnI CCSGG 2 cut(s) 142, 781
BcoDI GTCTC 1 cut(s) 434
BfaI CTAG 2 cut(s) 227, 371
BfmI CTRYAG 1 cut(s) 1023
BfoI RGCGCY 2 cut(s) 611, 706
BfuAI ACCTGC 2 cut(s) 325, 1152
BglII AGATCT 1 cut(s) 849
BlpI GCTNAGC 1 cut(s) 125
Bme1390I CCNGG 5 cut(s) 142, 161, 475, 496, 781
Bme18I GGWCC 2 cut(s) 152, 252
BmeT110I CYCGRG 1 cut(s) 947
BmgT120I GGNCC 3 cut(s) 152, 252, 266
BmiI GGNNCC 6 cut(s) 153, 154, 253, 254, 268, 609
BmrFI CCNGG 5 cut(s) 142, 161, 475, 496, 781
BmsI GCATC 3 cut(s) 27, 541, 563
BpiI GAAGAC 1 cut(s) 699
Bpu1102I GCTNAGC 1 cut(s) 125
BpuEI CTTGAG 2 cut(s) 167, 387
BpuMI CCSGG 2 cut(s) 142, 781
Bsa29I ATCGAT 1 cut(s) 793
BsaAI YACGTR 1 cut(s) 118
BsaBI GATNNNNATC 1 cut(s) 795
BsaHI GRCGYC 3 cut(s) 36, 410, 608
BsaJI CCNNGG 4 cut(s) 159, 258, 473, 948
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 6 cut(s) 263, 274, 457, 627, 911, 1160
Bse118I RCCGGY 2 cut(s) 1037, 1160
Bse1I ACTGG 2 cut(s) 812, 900
Bse8I GATNNNNATC 1 cut(s) 795
BseBI CCWGG 3 cut(s) 161, 475, 496
BseCI ATCGAT 1 cut(s) 793
BseDI CCNNGG 4 cut(s) 159, 258, 473, 948
BseGI GGATG 2 cut(s) 111, 1084
BseJI GATNNNNATC 1 cut(s) 795
BseLI CCNNNNNNNGG 6 cut(s) 263, 274, 457, 627, 911, 1160
BseMII CTCAG 5 cut(s) 116, 384, 531, 675, 765
BseNI ACTGG 2 cut(s) 812, 900
BseRI GAGGAG 2 cut(s) 746, 1069
BseSI GKGCMC 2 cut(s) 303, 717
BseYI CCCAGC 1 cut(s) 222
BshFI GGCC 2 cut(s) 267, 450
BshNI GGYRCC 1 cut(s) 607
BshVI ATCGAT 1 cut(s) 793
BsiHKAI GWGCWC 3 cut(s) 303, 599, 717
BsiHKCI CYCGRG 1 cut(s) 947
BsiSI CCGG 4 cut(s) 141, 780, 1038, 1161
BslFI GGGAC 3 cut(s) 138, 238, 906
BslI CCNNNNNNNGG 6 cut(s) 263, 274, 457, 627, 911, 1160
BsmAI GTCTC 1 cut(s) 434
BsmBI CGTCTC 1 cut(s) 434
BsmFI GGGAC 3 cut(s) 138, 238, 906
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 2 cut(s) 267, 450
BsoBI CYCGRG 1 cut(s) 947
Bsp1286I GDGCHC 3 cut(s) 303, 599, 717
Bsp143I GATC 9 cut(s) 4, 132, 199, 237, 460, 790, 849, 1081, 1101
Bsp1720I GCTNAGC 1 cut(s) 125
BspACI CCGC 4 cut(s) 104, 264, 447, 700
BspANI GGCC 2 cut(s) 267, 450
BspCNI CTCAG 5 cut(s) 117, 385, 532, 674, 766
BspDI ATCGAT 1 cut(s) 793
BspHI TCATGA 1 cut(s) 615
BspLI GGNNCC 6 cut(s) 153, 154, 253, 254, 268, 609
BspMAI CTGCAG 1 cut(s) 1027
BspMI ACCTGC 2 cut(s) 325, 1152
BspPI GGATC 3 cut(s) 12, 468, 798
BspT107I GGYRCC 1 cut(s) 607
BsrBI CCGCTC 1 cut(s) 447
BsrFI RCCGGY 2 cut(s) 1037, 1160
BsrI ACTGG 2 cut(s) 812, 900
BssAI RCCGGY 2 cut(s) 1037, 1160
BssECI CCNNGG 4 cut(s) 159, 258, 473, 948
BssMI GATC 9 cut(s) 4, 132, 199, 237, 460, 790, 849, 1081, 1101
BssNI GRCGYC 3 cut(s) 36, 410, 608
BssSI CACGAG 1 cut(s) 92
Bst2BI CACGAG 1 cut(s) 92
Bst2UI CCWGG 3 cut(s) 161, 475, 496
Bst4CI ACNGT 3 cut(s) 601, 656, 1126
Bst6I CTCTTC 1 cut(s) 1100
BstACI GRCGYC 3 cut(s) 36, 410, 608
BstBAI YACGTR 1 cut(s) 118
BstC8I GCNNGC 5 cut(s) 80, 276, 750, 1039, 1162
BstDEI CTNAG 7 cut(s) 8, 125, 393, 540, 661, 667, 774
BstDSI CCRYGG 1 cut(s) 258
BstEII GGTNACC 1 cut(s) 1139
BstENI CCTNNNNNAGG 1 cut(s) 455
BstF5I GGATG 2 cut(s) 111, 1084
BstH2I RGCGCY 2 cut(s) 611, 706
BstHHI GCGC 2 cut(s) 610, 705
BstKTI GATC 9 cut(s) 7, 135, 202, 240, 463, 793, 852, 1084, 1104
BstMAI GTCTC 1 cut(s) 434
BstMBI GATC 9 cut(s) 4, 132, 199, 237, 460, 790, 849, 1081, 1101
BstMWI GCNNNNNNNGC 7 cut(s) 55, 298, 313, 365, 407, 1028, 1034
BstNI CCWGG 3 cut(s) 161, 475, 496
BstPI GGTNACC 1 cut(s) 1139
BstSCI CCNGG 5 cut(s) 140, 159, 473, 494, 779
BstSFI CTRYAG 1 cut(s) 1023
BstSLI GKGCMC 2 cut(s) 303, 717
BstV2I GAAGAC 1 cut(s) 699
BstX2I RGATCY 3 cut(s) 4, 460, 849
BstXI CCANNNNNNTGG 1 cut(s) 118
BstYI RGATCY 3 cut(s) 4, 460, 849
Bsu15I ATCGAT 1 cut(s) 793
BsuRI GGCC 2 cut(s) 267, 450
BsuTUI ATCGAT 1 cut(s) 793
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 2 cut(s) 111, 1084
BtsI GCAGTG 1 cut(s) 825
BtsIMutI CAGTG 1 cut(s) 825
BveI ACCTGC 2 cut(s) 325, 1152
Cac8I GCNNGC 5 cut(s) 80, 276, 750, 1039, 1162
CaiI CAGNNNCTG 1 cut(s) 1022
CciI TCATGA 1 cut(s) 615
CfoI GCGC 2 cut(s) 610, 705
Cfr10I RCCGGY 2 cut(s) 1037, 1160
Cfr13I GGNCC 3 cut(s) 152, 252, 266
ClaI ATCGAT 1 cut(s) 793
CseI GACGC 2 cut(s) 353, 399
Csp6I GTAC 2 cut(s) 97, 520
CviAII CATG 4 cut(s) 426, 572, 616, 1066
CviQI GTAC 2 cut(s) 97, 520
DdeI CTNAG 7 cut(s) 8, 125, 393, 540, 661, 667, 774
DinI GGCGCC 1 cut(s) 609
DpnI GATC 9 cut(s) 6, 134, 201, 239, 462, 792, 851, 1083, 1103
DpnII GATC 9 cut(s) 4, 132, 199, 237, 460, 790, 849, 1081, 1101
DraIII CACNNNGTG 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 1100
EarI CTCTTC 1 cut(s) 1100
Eco32I GATATC 1 cut(s) 797
Eco47I GGWCC 2 cut(s) 152, 252
Eco57I CTGAAG 2 cut(s) 338, 814
Eco72I CACGTG 1 cut(s) 118
Eco88I CYCGRG 1 cut(s) 947
Eco91I GGTNACC 1 cut(s) 1139
EcoNI CCTNNNNNAGG 1 cut(s) 455
EcoO109I RGGNCCY 2 cut(s) 152, 252
EcoO65I GGTNACC 1 cut(s) 1139
EcoRI GAATTC 1 cut(s) 421
EcoRII CCWGG 3 cut(s) 159, 473, 494
EcoRV GATATC 1 cut(s) 797
EcoT22I ATGCAT 2 cut(s) 517, 556
EgeI GGCGCC 1 cut(s) 609
EheI GGCGCC 1 cut(s) 609
Esp3I CGTCTC 1 cut(s) 434
FaeI CATG 4 cut(s) 429, 575, 619, 1069
FalI AAGNNNNNCTT 6 cut(s) 311, 343, 596, 628, 689, 721
FaqI GGGAC 3 cut(s) 138, 238, 906
FatI CATG 4 cut(s) 425, 571, 615, 1065
FbaI TGATCA 2 cut(s) 132, 1081
FokI GGATG 2 cut(s) 98, 1071
FspBI CTAG 2 cut(s) 227, 371
GlaI GCGC 2 cut(s) 609, 704
GsaI CCCAGC 1 cut(s) 226
HaeII RGCGCY 2 cut(s) 611, 706
HaeIII GGCC 2 cut(s) 267, 450
HapII CCGG 4 cut(s) 141, 780, 1038, 1161
HgaI GACGC 2 cut(s) 353, 399
HhaI GCGC 2 cut(s) 610, 705
Hin1I GRCGYC 3 cut(s) 36, 410, 608
Hin1II CATG 4 cut(s) 429, 575, 619, 1069
Hin6I GCGC 2 cut(s) 608, 703
HinP1I GCGC 2 cut(s) 608, 703
HindIII AAGCTT 1 cut(s) 867
HinfI GANTC 4 cut(s) 776, 834, 886, 941
HpaII CCGG 4 cut(s) 141, 780, 1038, 1161
HphI GGTGA 2 cut(s) 194, 926
Hpy166II GTNNAC 6 cut(s) 97, 195, 301, 343, 715, 940
Hpy188I TCNGA 8 cut(s) 9, 69, 247, 384, 775, 833, 927, 1053
Hpy188III TCNNGA 5 cut(s) 414, 587, 616, 728, 1099
Hpy8I GTNNAC 6 cut(s) 97, 195, 301, 343, 715, 940
Hpy99I CGWCG 4 cut(s) 38, 41, 415, 593
HpyAV CCTTC 8 cut(s) 68, 223, 333, 337, 593, 622, 911, 1004
HpyCH4III ACNGT 3 cut(s) 601, 656, 1126
HpyCH4IV ACGT 2 cut(s) 36, 117
HpyCH4V TGCA 6 cut(s) 301, 515, 554, 715, 818, 1025
HpyF10VI GCNNNNNNNGC 7 cut(s) 55, 298, 313, 365, 407, 1028, 1034
HpyF3I CTNAG 7 cut(s) 8, 125, 393, 540, 661, 667, 774
HpySE526I ACGT 2 cut(s) 36, 117
Hsp92I GRCGYC 3 cut(s) 36, 410, 608
Hsp92II CATG 4 cut(s) 429, 575, 619, 1069
HspAI GCGC 2 cut(s) 608, 703
KasI GGCGCC 1 cut(s) 607
KflI GGGWCCC 2 cut(s) 152, 252
KroI GCCGGC 2 cut(s) 1037, 1160
KroNI GCCGGC 2 cut(s) 1039, 1162
Ksp22I TGATCA 2 cut(s) 132, 1081
Kzo9I GATC 9 cut(s) 4, 132, 199, 237, 460, 790, 849, 1081, 1101
LmnI GCTCC 3 cut(s) 163, 530, 594
LweI GCATC 3 cut(s) 27, 541, 563
MaeI CTAG 2 cut(s) 227, 371
MaeII ACGT 2 cut(s) 36, 117
MaeIII GTNAC 2 cut(s) 477, 1139
MalI GATC 9 cut(s) 6, 134, 201, 239, 462, 792, 851, 1083, 1103
MbiI CCGCTC 1 cut(s) 447
MboI GATC 9 cut(s) 4, 132, 199, 237, 460, 790, 849, 1081, 1101
MboII GAAGA 7 cut(s) 232, 704, 716, 791, 1019, 1087, 1121
MflI RGATCY 3 cut(s) 4, 460, 849
MhlI GDGCHC 3 cut(s) 303, 599, 717
MluCI AATT 2 cut(s) 421, 545
Mly113I GGCGCC 1 cut(s) 608
MlyI GAGTC 3 cut(s) 785, 895, 935
Mph1103I ATGCAT 2 cut(s) 517, 556
MroNI GCCGGC 2 cut(s) 1037, 1160
MseI TTAA 2 cut(s) 603, 846
MslI CAYNNNNRTG 2 cut(s) 116, 998
MspA1I CMGCKG 4 cut(s) 61, 106, 169, 1028
MspI CCGG 4 cut(s) 141, 780, 1038, 1161
MspR9I CCNGG 5 cut(s) 142, 161, 475, 496, 781
Mva1269I GAATGC 1 cut(s) 517
MvaI CCWGG 3 cut(s) 161, 475, 496
MwoI GCNNNNNNNGC 7 cut(s) 55, 298, 313, 365, 407, 1028, 1034
NaeI GCCGGC 2 cut(s) 1039, 1162
NarI GGCGCC 1 cut(s) 608
NciI CCSGG 2 cut(s) 142, 781
NdeII GATC 9 cut(s) 4, 132, 199, 237, 460, 790, 849, 1081, 1101
NgoMIV GCCGGC 2 cut(s) 1037, 1160
NlaIII CATG 4 cut(s) 429, 575, 619, 1069
NlaIV GGNNCC 6 cut(s) 153, 154, 253, 254, 268, 609
NmuCI GTSAC 1 cut(s) 477
NsiI ATGCAT 2 cut(s) 517, 556
PagI TCATGA 1 cut(s) 615
PaqCI CACCTGC 1 cut(s) 325
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PctI GAATGC 1 cut(s) 517
PdiI GCCGGC 2 cut(s) 1039, 1162
PfeI GAWTC 1 cut(s) 834
PleI GAGTC 3 cut(s) 784, 894, 935
PluTI GGCGCC 1 cut(s) 611
PmaCI CACGTG 1 cut(s) 118
PmlI CACGTG 1 cut(s) 118
PpsI GAGTC 3 cut(s) 784, 894, 935
Ppu21I YACGTR 1 cut(s) 118
PpuMI RGGWCCY 2 cut(s) 152, 252
PsiI TTATAA 1 cut(s) 1046
Psp5II RGGWCCY 2 cut(s) 152, 252
Psp6I CCWGG 3 cut(s) 159, 473, 494
PspCI CACGTG 1 cut(s) 118
PspEI GGTNACC 1 cut(s) 1139
PspFI CCCAGC 1 cut(s) 222
PspGI CCWGG 3 cut(s) 159, 473, 494
PspN4I GGNNCC 6 cut(s) 153, 154, 253, 254, 268, 609
PspPI GGNCC 3 cut(s) 152, 252, 266
PspPPI RGGWCCY 2 cut(s) 152, 252
PsrI GAACNNNNNNTAC 2 cut(s) 80, 112
PstI CTGCAG 1 cut(s) 1027
PstNI CAGNNNCTG 1 cut(s) 1022
PsuI RGATCY 3 cut(s) 4, 460, 849
PvuII CAGCTG 3 cut(s) 61, 169, 1028
RsaI GTAC 2 cut(s) 98, 521
RsaNI GTAC 2 cut(s) 97, 520
RseI CAYNNNNRTG 2 cut(s) 116, 998
SaqAI TTAA 2 cut(s) 603, 846
Sau3AI GATC 9 cut(s) 4, 132, 199, 237, 460, 790, 849, 1081, 1101
Sau96I GGNCC 3 cut(s) 152, 252, 266
SchI GAGTC 3 cut(s) 785, 895, 935
ScrFI CCNGG 5 cut(s) 142, 161, 475, 496, 781
SduI GDGCHC 3 cut(s) 303, 599, 717
SfaNI GCATC 3 cut(s) 27, 541, 563
SfcI CTRYAG 1 cut(s) 1023
SfoI GGCGCC 1 cut(s) 609
SinI GGWCC 2 cut(s) 152, 252
SmiMI CAYNNNNRTG 2 cut(s) 116, 998
SmlI CTYRAG 2 cut(s) 182, 402
SmoI CTYRAG 2 cut(s) 182, 402
Sse9I AATT 2 cut(s) 421, 545
SsiI CCGC 4 cut(s) 104, 264, 447, 700
SspDI GGCGCC 1 cut(s) 607
SspMI CTAG 2 cut(s) 227, 371
StyD4I CCNGG 5 cut(s) 140, 159, 473, 494, 779
TaaI ACNGT 3 cut(s) 601, 656, 1126
TaiI ACGT 2 cut(s) 39, 120
TaqI TCGA 5 cut(s) 375, 588, 793, 909, 996
TaqII GACCGA 1 cut(s) 86
TasI AATT 2 cut(s) 421, 545
TatI WGTACW 2 cut(s) 96, 519
TauI GCSGC 4 cut(s) 106, 267, 450, 703
TfiI GAWTC 1 cut(s) 834
Tru1I TTAA 2 cut(s) 603, 846
Tru9I TTAA 2 cut(s) 603, 846
TscAI CASTG 1 cut(s) 825
TseFI GTSAC 1 cut(s) 477
Tsp45I GTSAC 1 cut(s) 477
TspDTI ATGAA 5 cut(s) 414, 604, 982, 1020, 1123
TspGWI ACGGA 2 cut(s) 223, 700
TspRI CASTG 1 cut(s) 825
VneI GTGCAC 2 cut(s) 299, 713
VpaK11BI GGWCC 2 cut(s) 152, 252
XagI CCTNNNNNAGG 1 cut(s) 455
XapI RAATTY 2 cut(s) 421, 545
XcmI CCANNNNNNNNNTGG 1 cut(s) 1072
XspI CTAG 2 cut(s) 227, 371
ZraI GACGTC 1 cut(s) 37
Zsp2I ATGCAT 2 cut(s) 517, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.