pycom08g03340

Belongs to the serpin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002697
Physical Location & Seq
Forward (+)
2598006 .. 2599172
1167 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03340.1

Sequence Viewer

Length: 822 bp
ATGGATCTGAGAGAAAGCATCATCAACCAAAACGACTTCGCTCTGGGGCTGACGAAGCAGCTGCTTCAGACCGAAGGCAAGCAATCGACCCTCGTGTACTCGCCGCTGTCCATCCACGTGCTGTTGAGCCTGATTGCGGCCGGGTCAAAGGGTCCGACTCAGGACCAGCTGCTGTCTTTTCTCAAATCAAAGTCCACTGACCACCTCAACTCCTTCGCCGCCGAGCTCGTGTCCATGATCTTCTCCGATGGGTCCCCTAGCGGCGGCCCCCATCTCTCGTTTGCGAGTGGAGTATGGGTGGACAGGTGTCTCCCTCTGAAGCCTTCTTTTAAGCAGGTGGTGGACACCTCTTACATGGCTGCTCTTGCCCAAGTCGATTTTCAGACCAATGCTGCTCAAGTGGCCTCGGGAGTGAATTCATGGGCTGAAAAGGAGACGAGAGGCCTTATCAAGGAGGTACTTCCTCCCGGGTCAGTTGACAGCTCAACCAGGCTCATCTTTGCAAATGCATTATACTTCAAAGGAGCTTGGACTGAAAAGTTTGATGCATCACAAACTAAAGAGCATGACTTCCACCTTCTCGATGGGAGCACAGTTCAGGTGCCATTTATGACCAGCAGGAAGAAACAGTATGTAAGTTCCTTCAATGGTTTCTCAGTCTTAGGGCTTCATTACAAGCAAGGTGAAGACAAGCGGTGTTTCTCCATGTACATCTTTCTTCCCGAGGCGAGATATGGGCTCCCAGCTTTGGTTGAGAAACTTGGTTCTGAGTCCGGGTTTTTGGATCGACCATCTTCACAAGCAAAAATCTGCAGTGGGTGA

Protein Analysis

274

Amino Acids

29.86

Weight (kDa)

7.01

Isoelectric Point (pI)

32.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 9 - 258 6.1e-61 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 325
Acc36I ACCTGC 1 cut(s) 325
AccB1I GGYRCC 1 cut(s) 601
AciI CCGC 6 cut(s) 104, 137, 219, 261, 264, 694
AclWI GGATC 2 cut(s) 12, 792
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 1 cut(s) 415
AcuI CTGAAG 2 cut(s) 50, 338
AcvI CACGTG 1 cut(s) 118
AfaI GTAC 3 cut(s) 98, 459, 710
AfiI CCNNNNNNNGG 4 cut(s) 136, 263, 451, 748
AgsI TTSAA 2 cut(s) 520, 646
AjnI CCWGG 1 cut(s) 488
AjuI GAANNNNNNNTTGG 2 cut(s) 363, 395
AluBI AGCT 6 cut(s) 61, 169, 226, 483, 527, 746
AluI AGCT 6 cut(s) 61, 169, 226, 483, 527, 746
Alw21I GWGCWC 2 cut(s) 228, 593
Alw26I GTCTC 2 cut(s) 314, 428
AlwI GGATC 2 cut(s) 12, 792
AlwNI CAGNNNCTG 1 cut(s) 172
Ama87I CYCGRG 3 cut(s) 406, 467, 722
AoxI GGCC 4 cut(s) 138, 265, 402, 442
ApeKI GCWGC 5 cut(s) 58, 61, 169, 359, 392
ApoI RAATTY 1 cut(s) 415
AspS9I GGNCC 4 cut(s) 152, 163, 252, 266
AsuC2I CCSGG 4 cut(s) 142, 468, 469, 775
AsuHPI GGTGA 1 cut(s) 695
AvaI CYCGRG 3 cut(s) 406, 467, 722
AvaII GGWCC 3 cut(s) 152, 163, 252
BanI GGYRCC 1 cut(s) 601
BanII GRGCYC 2 cut(s) 228, 741
BauI CACGAG 2 cut(s) 92, 227
BbrPI CACGTG 1 cut(s) 118
BbsI GAAGAC 1 cut(s) 693
Bbv12I GWGCWC 2 cut(s) 228, 593
BbvI GCAGC 5 cut(s) 48, 70, 156, 346, 379
BccI CCATC 5 cut(s) 119, 242, 279, 578, 799
BcgI CGANNNNNNTGC 2 cut(s) 43, 77
BciT130I CCWGG 1 cut(s) 490
BcnI CCSGG 4 cut(s) 142, 468, 469, 775
BcoDI GTCTC 2 cut(s) 314, 428
BfaI CTAG 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 811
BfuAI ACCTGC 1 cut(s) 325
Bme1390I CCNGG 5 cut(s) 142, 468, 469, 490, 775
Bme18I GGWCC 3 cut(s) 152, 163, 252
BmeT110I CYCGRG 3 cut(s) 406, 467, 722
BmgT120I GGNCC 4 cut(s) 152, 163, 252, 266
BmiI GGNNCC 6 cut(s) 153, 253, 254, 268, 603, 740
BmrFI CCNGG 5 cut(s) 142, 468, 469, 490, 775
BmsI GCATC 3 cut(s) 27, 535, 557
BoxI GACNNNNGTC 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 693
BpuEI CTTGAG 1 cut(s) 381
BpuMI CCSGG 4 cut(s) 142, 468, 469, 775
BsaAI YACGTR 1 cut(s) 118
BsaJI CCNNGG 3 cut(s) 405, 467, 723
BsaXI ACNNNNNCTCC 4 cut(s) 194, 224, 282, 312
Bsc4I CCNNNNNNNGG 4 cut(s) 136, 263, 451, 748
BseBI CCWGG 1 cut(s) 490
BseDI CCNNGG 3 cut(s) 405, 467, 723
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 4 cut(s) 136, 263, 451, 748
BseMII CTCAG 3 cut(s) 173, 669, 759
BseX3I CGGCCG 1 cut(s) 138
BseXI GCAGC 5 cut(s) 48, 70, 156, 346, 379
BseYI CCCAGC 1 cut(s) 742
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 4 cut(s) 140, 267, 404, 444
BshNI GGYRCC 1 cut(s) 601
BsiEI CGRYCG 1 cut(s) 141
BsiHKAI GWGCWC 2 cut(s) 228, 593
BsiHKCI CYCGRG 3 cut(s) 406, 467, 722
BsiSI CCGG 3 cut(s) 141, 468, 774
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 4 cut(s) 136, 263, 451, 748
BsmAI GTCTC 2 cut(s) 314, 428
BsmBI CGTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 4 cut(s) 140, 267, 404, 444
BsoBI CYCGRG 3 cut(s) 406, 467, 722
Bsp1286I GDGCHC 3 cut(s) 228, 593, 741
Bsp1407I TGTACA 1 cut(s) 708
Bsp143I GATC 3 cut(s) 4, 237, 784
BspACI CCGC 6 cut(s) 104, 137, 219, 261, 264, 694
BspANI GGCC 4 cut(s) 140, 267, 404, 444
BspCNI CTCAG 3 cut(s) 172, 668, 760
BspLI GGNNCC 6 cut(s) 153, 253, 254, 268, 603, 740
BspMAI CTGCAG 1 cut(s) 815
BspMI ACCTGC 1 cut(s) 325
BspPI GGATC 2 cut(s) 12, 792
BspT107I GGYRCC 1 cut(s) 601
BsrGI TGTACA 1 cut(s) 708
BssECI CCNNGG 3 cut(s) 405, 467, 723
BssMI GATC 3 cut(s) 4, 237, 784
BssSI CACGAG 2 cut(s) 92, 227
Bst2BI CACGAG 2 cut(s) 92, 227
Bst2UI CCWGG 1 cut(s) 490
Bst4CI ACNGT 2 cut(s) 595, 630
BstAUI TGTACA 1 cut(s) 708
BstBAI YACGTR 1 cut(s) 118
BstC8I GCNNGC 1 cut(s) 80
BstDEI CTNAG 5 cut(s) 8, 159, 655, 661, 768
BstENI CCTNNNNNAGG 1 cut(s) 449
BstF5I GGATG 1 cut(s) 111
BstKTI GATC 3 cut(s) 7, 240, 787
BstMAI GTCTC 2 cut(s) 314, 428
BstMBI GATC 3 cut(s) 4, 237, 784
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 3 cut(s) 55, 365, 401
BstNI CCWGG 1 cut(s) 490
BstPAI GACNNNNGTC 1 cut(s) 306
BstSCI CCNGG 5 cut(s) 140, 466, 467, 488, 773
BstSFI CTRYAG 1 cut(s) 811
BstV1I GCAGC 5 cut(s) 48, 70, 156, 346, 379
BstV2I GAAGAC 1 cut(s) 693
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZI CGGCCG 1 cut(s) 138
BsuRI GGCC 4 cut(s) 140, 267, 404, 444
BtsCI GGATG 1 cut(s) 111
BtsI GCAGTG 1 cut(s) 820
BtsIMutI CAGTG 2 cut(s) 195, 820
BveI ACCTGC 1 cut(s) 325
Cac8I GCNNGC 1 cut(s) 80
CaiI CAGNNNCTG 1 cut(s) 172
Cfr13I GGNCC 4 cut(s) 152, 163, 252, 266
Cfr9I CCCGGG 1 cut(s) 467
Csp6I GTAC 3 cut(s) 97, 458, 709
CviAII CATG 5 cut(s) 235, 355, 420, 566, 706
CviQI GTAC 3 cut(s) 97, 458, 709
DdeI CTNAG 5 cut(s) 8, 159, 655, 661, 768
DpnI GATC 3 cut(s) 6, 239, 786
DpnII GATC 3 cut(s) 4, 237, 784
EaeI YGGCCR 1 cut(s) 138
EagI CGGCCG 1 cut(s) 138
Ecl136II GAGCTC 1 cut(s) 226
EclXI CGGCCG 1 cut(s) 138
Eco147I AGGCCT 1 cut(s) 444
Eco24I GRGCYC 2 cut(s) 228, 741
Eco47I GGWCC 3 cut(s) 152, 163, 252
Eco52I CGGCCG 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 2 cut(s) 50, 338
Eco72I CACGTG 1 cut(s) 118
Eco88I CYCGRG 3 cut(s) 406, 467, 722
EcoICRI GAGCTC 1 cut(s) 226
EcoNI CCTNNNNNAGG 1 cut(s) 449
EcoO109I RGGNCCY 1 cut(s) 252
EcoRI GAATTC 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 488
EcoT22I ATGCAT 2 cut(s) 511, 550
EcoT38I GRGCYC 2 cut(s) 228, 741
Esp3I CGTCTC 1 cut(s) 428
FaeI CATG 5 cut(s) 238, 358, 423, 569, 709
FaqI GGGAC 1 cut(s) 238
FatI CATG 5 cut(s) 234, 354, 419, 565, 705
FokI GGATG 1 cut(s) 98
FriOI GRGCYC 2 cut(s) 228, 741
FspBI CTAG 1 cut(s) 258
GsaI CCCAGC 1 cut(s) 746
HaeIII GGCC 4 cut(s) 140, 267, 404, 444
HapII CCGG 3 cut(s) 141, 468, 774
Hin1II CATG 5 cut(s) 238, 358, 423, 569, 709
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HinfI GANTC 2 cut(s) 157, 770
HpaII CCGG 3 cut(s) 141, 468, 774
HphI GGTGA 1 cut(s) 695
Hpy166II GTNNAC 5 cut(s) 97, 195, 301, 343, 478
Hpy188I TCNGA 7 cut(s) 9, 69, 156, 247, 318, 384, 769
Hpy188III TCNNGA 4 cut(s) 161, 408, 581, 722
Hpy8I GTNNAC 5 cut(s) 97, 195, 301, 343, 478
HpyAV CCTTC 5 cut(s) 68, 223, 333, 587, 652
HpyCH4III ACNGT 2 cut(s) 595, 630
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 4 cut(s) 503, 509, 548, 813
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 365, 401
HpyF3I CTNAG 5 cut(s) 8, 159, 655, 661, 768
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 5 cut(s) 238, 358, 423, 569, 709
KflI GGGWCCC 1 cut(s) 252
Kzo9I GATC 3 cut(s) 4, 237, 784
LmnI GCTCC 3 cut(s) 524, 588, 744
Lsp1109I GCAGC 5 cut(s) 48, 70, 156, 346, 379
LweI GCATC 3 cut(s) 27, 535, 557
MaeI CTAG 1 cut(s) 258
MaeII ACGT 1 cut(s) 117
MalI GATC 3 cut(s) 6, 239, 786
MboI GATC 3 cut(s) 4, 237, 784
MboII GAAGA 5 cut(s) 232, 634, 698, 710, 786
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 3 cut(s) 228, 593, 741
MluCI AATT 1 cut(s) 415
MlyI GAGTC 2 cut(s) 151, 779
MmeI TCCRAC 1 cut(s) 179
MnlI CCTC 9 cut(s) 101, 215, 324, 358, 415, 434, 448, 474, 718
Mph1103I ATGCAT 2 cut(s) 511, 550
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 116
MspA1I CMGCKG 3 cut(s) 61, 106, 169
MspI CCGG 3 cut(s) 141, 468, 774
MspR9I CCNGG 5 cut(s) 142, 468, 469, 490, 775
MvaI CCWGG 1 cut(s) 490
MwoI GCNNNNNNNGC 3 cut(s) 55, 365, 401
NciI CCSGG 4 cut(s) 142, 468, 469, 775
NdeII GATC 3 cut(s) 4, 237, 784
NlaIII CATG 5 cut(s) 238, 358, 423, 569, 709
NlaIV GGNNCC 6 cut(s) 153, 253, 254, 268, 603, 740
NmeAIII GCCGAG 1 cut(s) 247
NsiI ATGCAT 2 cut(s) 511, 550
PaqCI CACCTGC 1 cut(s) 325
PceI AGGCCT 1 cut(s) 444
PleI GAGTC 2 cut(s) 151, 778
PmaCI CACGTG 1 cut(s) 118
PmlI CACGTG 1 cut(s) 118
PpsI GAGTC 2 cut(s) 151, 778
Ppu21I YACGTR 1 cut(s) 118
PpuMI RGGWCCY 1 cut(s) 252
PshAI GACNNNNGTC 1 cut(s) 306
Psp124BI GAGCTC 1 cut(s) 228
Psp5II RGGWCCY 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 488
PspCI CACGTG 1 cut(s) 118
PspFI CCCAGC 1 cut(s) 742
PspGI CCWGG 1 cut(s) 488
PspN4I GGNNCC 6 cut(s) 153, 253, 254, 268, 603, 740
PspPI GGNCC 4 cut(s) 152, 163, 252, 266
PspPPI RGGWCCY 1 cut(s) 252
PstI CTGCAG 1 cut(s) 815
PstNI CAGNNNCTG 1 cut(s) 172
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 2 cut(s) 61, 169
RsaI GTAC 3 cut(s) 98, 459, 710
RsaNI GTAC 3 cut(s) 97, 458, 709
RseI CAYNNNNRTG 1 cut(s) 116
SacI GAGCTC 1 cut(s) 228
SaqAI TTAA 1 cut(s) 330
Sau3AI GATC 3 cut(s) 4, 237, 784
Sau96I GGNCC 4 cut(s) 152, 163, 252, 266
SchI GAGTC 2 cut(s) 151, 779
ScrFI CCNGG 5 cut(s) 142, 468, 469, 490, 775
SduI GDGCHC 3 cut(s) 228, 593, 741
SfaNI GCATC 3 cut(s) 27, 535, 557
SfcI CTRYAG 1 cut(s) 811
SinI GGWCC 3 cut(s) 152, 163, 252
SmaI CCCGGG 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
Sse9I AATT 1 cut(s) 415
SseBI AGGCCT 1 cut(s) 444
SsiI CCGC 6 cut(s) 104, 137, 219, 261, 264, 694
SspMI CTAG 1 cut(s) 258
SstI GAGCTC 1 cut(s) 228
StuI AGGCCT 1 cut(s) 444
StyD4I CCNGG 5 cut(s) 140, 466, 467, 488, 773
TaaI ACNGT 2 cut(s) 595, 630
TaiI ACGT 1 cut(s) 120
TaqI TCGA 4 cut(s) 86, 375, 582, 787
TaqII GACCGA 1 cut(s) 86
TasI AATT 1 cut(s) 415
TatI WGTACW 2 cut(s) 96, 708
TauI GCSGC 5 cut(s) 106, 140, 221, 264, 267
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 2 cut(s) 202, 820
TseI GCWGC 5 cut(s) 58, 61, 169, 359, 392
TspDTI ATGAA 2 cut(s) 408, 659
TspMI CCCGGG 1 cut(s) 467
TspRI CASTG 2 cut(s) 202, 820
VpaK11BI GGWCC 3 cut(s) 152, 163, 252
XagI CCTNNNNNAGG 1 cut(s) 449
XapI RAATTY 1 cut(s) 415
XcmI CCANNNNNNNNNTGG 1 cut(s) 581
XmaI CCCGGG 1 cut(s) 467
XspI CTAG 1 cut(s) 258
Zsp2I ATGCAT 2 cut(s) 511, 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.