Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002697

Physical Location & Seq

Genomic Coordinates Forward (+)

2472316 .. 2475056

Gene Structure 2741 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1035000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1167 bp

ATGGATCTGCGAGAAAGCATCATCAACCAAACCGACGTCGCACTGGGGCTGACGAAGAAGCTGCTTCAGACCGAAGGCAAGCAATCCAACCTTGTGTACTCGCCGCTGTCCATCCACGTGGTGCTGAGCCTGATCGCGGCCGGGTCAAAGGGTCCGACCCAGGACCAGCTCCTCTCGTTCCTCAAGTCAAAGTCCGCCGACCACCTCAACTCCTTCGCCGCCGAGCTCGTATCCGTGATCTTCTCCGATGGGTCCCCCAGCGGCGGCCCCCAGCTGGCGTTTGCGAATGGGGTTTGGGTGGACAAGCCTCTCCCTCTCAAGCCTTCTTTCAAACAGGTGGTGGACACTTCTTACAAGGCCGCTCTGGCCCAAGTCGATTTCCAGACCAATGCTGCTGAAGTGGCGTTGGGAGTGAATTCATGGGCTGAAAAGAAGACGAGCGGACTTATCAAGGAGGTACTTCCTCCCGGGTCAGTTGACAGCTCAACCAGGCTCATATTTGCTAATGCATTATACTTCAAAGGAGCTTGGAATGAAAAGTTTGATGCATCGACTACAAAAGAGCATGACTTCCACCTACTCGATGGGAGCACAGTTAAGGCACCCTTCATGACCAGCAAGAAGAAGCAGTATGTAAGTGCCTATGACGGTTTCTCAGTCTTGGGGCTTCCTTACAAACAAGGCGAAGACAAGCGGCGCTTTTCCATGCACATCTTCCTTCCGGAGGTGAAAGATGGGCTGCCAGCTTTGGTTGACAAACTTGGTTCTGAGTCTGGCTTTTTGGATCGCCATCTTCCCAAAAGCCAAGTTGCAGTGGGCGACTTCAGAATCCCAAAGTTTAAGATCTCATTCGGGTTTGAAGCTTCCAATGTCCTTAAGGGTCTGGGACTGGTGCTGCCTTTCGGTGGTGAAGGAGGTTTGACAGAGATGGTGGACTCGCCTGAGGGAAAGAACCTATACGTTTCCAGCATATTCCACAAGTCGTTCATCGAAGTGAATGAAGAAGGCACAGAAGCTGCAGCTTCTTCTGCCGGCGTTATAAAACTAAGGGGTATTTCCATGACAGTTGACTTTGTGGCTGATCGTCCATTCCTCTTCCTGATCAGGGAAGAAATGACCGGAACAGTTCTGTTCATCGGGCACGTGCTCAACCCTCTTGCCGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0004857 GO:0004866 GO:0004867 GO:0004869 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0009892 GO:0010466 GO:0010605 GO:0010951 GO:0019222 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0043086 GO:0044092 GO:0044421 GO:0044424 GO:0044464 GO:0045861 GO:0048046 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko05146 map05146

Pfam Domains

Protein Families

Serpin

Protein Analysis

389

Amino Acids

41.95

Weight (kDa)

6.47

Isoelectric Point (pI)

31.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Serpin	PF00079	10 - 385	3.1e-99	Serpin (serine protease inhibitor)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002697)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47710 AT1G47710 AT1G51330 AT1G62160 AT1G62170 AT1G62170 AT1G63280 AT1G64010 AT1G64010 AT1G64030 AT2G14540 AT2G25240 AT2G26390 AT2G35580 AT3G45220
fragaria_vesca	FvH4_2g40700
malus_domestica	MD08G1040600.v1.1 MD08G1041200.v1.1 MD08G1041300.v1.1 MD08G1041500.v1.1 MD08G1041600.v1.1 MD14G1097300.v1.1 MD15G1035000.v1.1 MD15G1286900.v1.1
prunus_persica	Prupe.1G389200_v2.0.a1 Prupe.1G389300_v2.0.a1 Prupe.3G141200_v2.0.a1
pyrus_communis	pycom08g03320 pycom08g03330 pycom08g03340 pycom08g03360 pycom15g03220
rosa_chinensis	RchiOBHm_Chr6g0299901
rosa_laevigata	RLG00000011377
rosa_multiflora	Rmu_ssc0000158.1_g000016
rosa_roxburghii	Rroxscaffold_7G00168290
rosa_rugosa	Rorug06G0293100
rosa_samantha	Rh4AG219100
rosa_wichuraiana	Rw6G035390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1040
AatII	GACGTC	1 cut(s)	39
AccB1I	GGYRCC	1 cut(s)	601
AccBSI	CCGCTC	2 cut(s)	362, 441
AccII	CGCG	1 cut(s)	137
AccIII	TCCGGA	1 cut(s)	721
AciI	CCGC	9 cut(s)	104, 137, 195, 219, 261, 264, 360, 441, 694
AclWI	GGATC	2 cut(s)	12, 792
AcoI	YGGCCR	1 cut(s)	138
AcsI	RAATTY	1 cut(s)	415
AcuI	CTGAAG	3 cut(s)	50, 417, 808
AcvI	CACGTG	2 cut(s)	118, 1144
AcyI	GRCGYC	1 cut(s)	36
AdeI	CACNNNGTG	1 cut(s)	121
AfaI	GTAC	2 cut(s)	98, 459
AfiI	CCNNNNNNNGG	7 cut(s)	136, 263, 274, 724, 905, 1105, 1160
AflII	CTTAAG	1 cut(s)	875
AgsI	TTSAA	3 cut(s)	331, 520, 860
AjnI	CCWGG	2 cut(s)	159, 488
Alw21I	GWGCWC	3 cut(s)	228, 593, 1149
AlwI	GGATC	2 cut(s)	12, 792
AlwNI	CAGNNNCTG	1 cut(s)	1016
Ama87I	CYCGRG	1 cut(s)	467
Aor13HI	TCCGGA	1 cut(s)	721
AoxI	GGCC	4 cut(s)	138, 265, 357, 366
ApeKI	GCWGC	6 cut(s)	61, 392, 739, 895, 1016, 1019
ApoI	RAATTY	1 cut(s)	415
AspLEI	GCGC	1 cut(s)	699
AspS9I	GGNCC	5 cut(s)	152, 163, 252, 266, 367
AsuC2I	CCSGG	3 cut(s)	142, 468, 469
AsuHPI	GGTGA	2 cut(s)	739, 920
AvaI	CYCGRG	1 cut(s)	467
AvaII	GGWCC	3 cut(s)	152, 163, 252
AxyI	CCTNAGG	1 cut(s)	942
BaeGI	GKGCMC	1 cut(s)	1143
BanI	GGYRCC	1 cut(s)	601
BanII	GRGCYC	1 cut(s)	228
BbrPI	CACGTG	2 cut(s)	118, 1144
BbsI	GAAGAC	2 cut(s)	440, 693
Bbv12I	GWGCWC	3 cut(s)	228, 593, 1149
BbvI	GCAGC	6 cut(s)	48, 379, 726, 882, 1003, 1031
BccI	CCATC	6 cut(s)	119, 242, 578, 728, 798, 922
BcgI	CGANNNNNNTGC	2 cut(s)	43, 77
BciT130I	CCWGG	2 cut(s)	161, 490
BciVI	GTATCC	1 cut(s)	241
BclI	TGATCA	1 cut(s)	1101
BcnI	CCSGG	3 cut(s)	142, 468, 469
BfmI	CTRYAG	1 cut(s)	1017
BfoI	RGCGCY	1 cut(s)	700
BfrI	CTTAAG	1 cut(s)	875
BfuI	GTATCC	1 cut(s)	241
BglI	GCCNNNNNGGC	1 cut(s)	365
BglII	AGATCT	1 cut(s)	843
BlpI	GCTNAGC	1 cut(s)	125
Bme1390I	CCNGG	5 cut(s)	142, 161, 468, 469, 490
Bme18I	GGWCC	3 cut(s)	152, 163, 252
BmeT110I	CYCGRG	1 cut(s)	467
BmgT120I	GGNCC	5 cut(s)	152, 163, 252, 266, 367
BmiI	GGNNCC	5 cut(s)	153, 253, 254, 268, 603
BmrFI	CCNGG	5 cut(s)	142, 161, 468, 469, 490
BmrI	ACTGGG	1 cut(s)	53
BmsI	GCATC	3 cut(s)	27, 535, 557
BmuI	ACTGGG	1 cut(s)	53
BpiI	GAAGAC	2 cut(s)	440, 693
Bpu1102I	GCTNAGC	1 cut(s)	125
BpuEI	CTTGAG	2 cut(s)	167, 302
BpuMI	CCSGG	3 cut(s)	142, 468, 469
BsaAI	YACGTR	2 cut(s)	118, 1144
BsaBI	GATNNNNATC	1 cut(s)	789
BsaHI	GRCGYC	1 cut(s)	36
BsaJI	CCNNGG	2 cut(s)	159, 467
BsaWI	WCCGGW	2 cut(s)	721, 1118
BsaXI	ACNNNNNCTCC	2 cut(s)	194, 224
Bsc4I	CCNNNNNNNGG	7 cut(s)	136, 263, 274, 724, 905, 1105, 1160
Bse118I	RCCGGY	2 cut(s)	1031, 1160
Bse1I	ACTGG	2 cut(s)	48, 894
Bse21I	CCTNAGG	1 cut(s)	942
Bse8I	GATNNNNATC	1 cut(s)	789
BseAI	TCCGGA	1 cut(s)	721
BseBI	CCWGG	2 cut(s)	161, 490
BseDI	CCNNGG	2 cut(s)	159, 467
BseGI	GGATG	1 cut(s)	111
BseJI	GATNNNNATC	1 cut(s)	789
BseLI	CCNNNNNNNGG	7 cut(s)	136, 263, 274, 724, 905, 1105, 1160
BseMII	CTCAG	4 cut(s)	116, 669, 759, 933
BseNI	ACTGG	2 cut(s)	48, 894
BseRI	GAGGAG	1 cut(s)	161
BseSI	GKGCMC	1 cut(s)	1143
BseX3I	CGGCCG	1 cut(s)	138
BseXI	GCAGC	6 cut(s)	48, 379, 726, 882, 1003, 1031
BseYI	CCCAGC	2 cut(s)	257, 270
Bsh1236I	CGCG	1 cut(s)	137
Bsh1285I	CGRYCG	1 cut(s)	141
BshFI	GGCC	4 cut(s)	140, 267, 359, 368
BshNI	GGYRCC	1 cut(s)	601
BsiEI	CGRYCG	1 cut(s)	141
BsiHKAI	GWGCWC	3 cut(s)	228, 593, 1149
BsiHKCI	CYCGRG	1 cut(s)	467
BsiSI	CCGG	6 cut(s)	141, 468, 722, 1032, 1119, 1161
BslFI	GGGAC	2 cut(s)	238, 900
BslI	CCNNNNNNNGG	7 cut(s)	136, 263, 274, 724, 905, 1105, 1160
BsmFI	GGGAC	2 cut(s)	238, 900
BsnI	GGCC	4 cut(s)	140, 267, 359, 368
BsoBI	CYCGRG	1 cut(s)	467
Bsp1286I	GDGCHC	4 cut(s)	228, 593, 1143, 1149
Bsp13I	TCCGGA	1 cut(s)	721
Bsp143I	GATC	7 cut(s)	4, 132, 237, 784, 843, 1081, 1101
Bsp1720I	GCTNAGC	1 cut(s)	125
BspACI	CCGC	9 cut(s)	104, 137, 195, 219, 261, 264, 360, 441, 694
BspANI	GGCC	4 cut(s)	140, 267, 359, 368
BspCNI	CTCAG	4 cut(s)	117, 668, 760, 934
BspEI	TCCGGA	1 cut(s)	721
BspFNI	CGCG	1 cut(s)	137
BspHI	TCATGA	1 cut(s)	609
BspLI	GGNNCC	5 cut(s)	153, 253, 254, 268, 603
BspMAI	CTGCAG	1 cut(s)	1021
BspPI	GGATC	2 cut(s)	12, 792
BspT107I	GGYRCC	1 cut(s)	601
BspTI	CTTAAG	1 cut(s)	875
BsrBI	CCGCTC	2 cut(s)	362, 441
BsrFI	RCCGGY	2 cut(s)	1031, 1160
BsrI	ACTGG	2 cut(s)	48, 894
BssAI	RCCGGY	2 cut(s)	1031, 1160
BssECI	CCNNGG	2 cut(s)	159, 467
BssMI	GATC	7 cut(s)	4, 132, 237, 784, 843, 1081, 1101
BssNI	GRCGYC	1 cut(s)	36
Bst2UI	CCWGG	2 cut(s)	161, 490
Bst4CI	ACNGT	4 cut(s)	595, 650, 1066, 1126
Bst6I	CTCTTC	1 cut(s)	1100
BstACI	GRCGYC	1 cut(s)	36
BstAFI	CTTAAG	1 cut(s)	875
BstBAI	YACGTR	2 cut(s)	118, 1144
BstC8I	GCNNGC	5 cut(s)	80, 276, 744, 1033, 1162
BstDEI	CTNAG	5 cut(s)	125, 655, 768, 942, 1046
BstENI	CCTNNNNNAGG	1 cut(s)	722
BstF5I	GGATG	1 cut(s)	111
BstFNI	CGCG	1 cut(s)	137
BstH2I	RGCGCY	1 cut(s)	700
BstHHI	GCGC	1 cut(s)	699
BstKTI	GATC	7 cut(s)	7, 135, 240, 787, 846, 1084, 1104
BstMBI	GATC	7 cut(s)	4, 132, 237, 784, 843, 1081, 1101
BstMCI	CGRYCG	1 cut(s)	141
BstMWI	GCNNNNNNNGC	3 cut(s)	365, 401, 1028
BstNI	CCWGG	2 cut(s)	161, 490
BstSCI	CCNGG	5 cut(s)	140, 159, 466, 467, 488
BstSFI	CTRYAG	1 cut(s)	1017
BstSLI	GKGCMC	1 cut(s)	1143
BstUI	CGCG	1 cut(s)	137
BstV1I	GCAGC	6 cut(s)	48, 379, 726, 882, 1003, 1031
BstV2I	GAAGAC	2 cut(s)	440, 693
BstX2I	RGATCY	2 cut(s)	4, 843
BstXI	CCANNNNNNTGG	1 cut(s)	118
BstYI	RGATCY	2 cut(s)	4, 843
BstZI	CGGCCG	1 cut(s)	138
Bsu36I	CCTNAGG	1 cut(s)	942
BsuI	GTATCC	1 cut(s)	241
BsuRI	GGCC	4 cut(s)	140, 267, 359, 368
BtsCI	GGATG	1 cut(s)	111
BtsI	GCAGTG	1 cut(s)	819
BtsIMutI	CAGTG	2 cut(s)	41, 819
Cac8I	GCNNGC	5 cut(s)	80, 276, 744, 1033, 1162
CaiI	CAGNNNCTG	1 cut(s)	1016
CciI	TCATGA	1 cut(s)	609
CfoI	GCGC	1 cut(s)	699
Cfr10I	RCCGGY	2 cut(s)	1031, 1160
Cfr13I	GGNCC	5 cut(s)	152, 163, 252, 266, 367
Cfr9I	CCCGGG	1 cut(s)	467
Csp6I	GTAC	2 cut(s)	97, 458
CviAII	CATG	5 cut(s)	420, 566, 610, 706, 1060
CviQI	GTAC	2 cut(s)	97, 458
DdeI	CTNAG	5 cut(s)	125, 655, 768, 942, 1046
DpnI	GATC	7 cut(s)	6, 134, 239, 786, 845, 1083, 1103
DpnII	GATC	7 cut(s)	4, 132, 237, 784, 843, 1081, 1101
DraIII	CACNNNGTG	1 cut(s)	121
EaeI	YGGCCR	1 cut(s)	138
EagI	CGGCCG	1 cut(s)	138
Eam1104I	CTCTTC	1 cut(s)	1100
EarI	CTCTTC	1 cut(s)	1100
EciI	GGCGGA	1 cut(s)	184
Ecl136II	GAGCTC	1 cut(s)	226
EclXI	CGGCCG	1 cut(s)	138
Eco24I	GRGCYC	1 cut(s)	228
Eco47I	GGWCC	3 cut(s)	152, 163, 252
Eco52I	CGGCCG	1 cut(s)	138
Eco53kI	GAGCTC	1 cut(s)	226
Eco57I	CTGAAG	3 cut(s)	50, 417, 808
Eco72I	CACGTG	2 cut(s)	118, 1144
Eco81I	CCTNAGG	1 cut(s)	942
Eco88I	CYCGRG	1 cut(s)	467
EcoICRI	GAGCTC	1 cut(s)	226
EcoNI	CCTNNNNNAGG	1 cut(s)	722
EcoO109I	RGGNCCY	1 cut(s)	252
EcoRI	GAATTC	1 cut(s)	415
EcoRII	CCWGG	2 cut(s)	159, 488
EcoT22I	ATGCAT	2 cut(s)	511, 550
EcoT38I	GRGCYC	1 cut(s)	228
FaeI	CATG	5 cut(s)	423, 569, 613, 709, 1063
FalI	AAGNNNNNCTT	4 cut(s)	590, 622, 683, 715
FaqI	GGGAC	2 cut(s)	238, 900
FatI	CATG	5 cut(s)	419, 565, 609, 705, 1059
FbaI	TGATCA	1 cut(s)	1101
FokI	GGATG	1 cut(s)	98
FriOI	GRGCYC	1 cut(s)	228
GlaI	GCGC	1 cut(s)	698
GsaI	CCCAGC	2 cut(s)	261, 274
HaeII	RGCGCY	1 cut(s)	700
HaeIII	GGCC	4 cut(s)	140, 267, 359, 368
HapII	CCGG	6 cut(s)	141, 468, 722, 1032, 1119, 1161
HhaI	GCGC	1 cut(s)	699
Hin1I	GRCGYC	1 cut(s)	36
Hin1II	CATG	5 cut(s)	423, 569, 613, 709, 1063
Hin6I	GCGC	1 cut(s)	697
HinP1I	GCGC	1 cut(s)	697
HincII	GTYRAC	3 cut(s)	478, 754, 1069
HindII	GTYRAC	3 cut(s)	478, 754, 1069
HindIII	AAGCTT	1 cut(s)	861
HinfI	GANTC	3 cut(s)	770, 828, 935
HpaII	CCGG	6 cut(s)	141, 468, 722, 1032, 1119, 1161
HphI	GGTGA	2 cut(s)	739, 920
Hpy166II	GTNNAC	7 cut(s)	97, 301, 343, 478, 754, 934, 1069
Hpy188I	TCNGA	5 cut(s)	69, 156, 247, 769, 827
Hpy188III	TCNNGA	4 cut(s)	382, 610, 722, 1099
Hpy8I	GTNNAC	7 cut(s)	97, 301, 343, 478, 754, 934, 1069
Hpy99I	CGWCG	2 cut(s)	38, 41
HpyAV	CCTTC	7 cut(s)	68, 223, 333, 616, 728, 905, 998
HpyCH4III	ACNGT	4 cut(s)	595, 650, 1066, 1126
HpyCH4IV	ACGT	4 cut(s)	36, 117, 960, 1143
HpyCH4V	TGCA	5 cut(s)	509, 548, 709, 812, 1019
HpyF10VI	GCNNNNNNNGC	3 cut(s)	365, 401, 1028
HpyF3I	CTNAG	5 cut(s)	125, 655, 768, 942, 1046
HpySE526I	ACGT	4 cut(s)	36, 117, 960, 1143
Hsp92I	GRCGYC	1 cut(s)	36
Hsp92II	CATG	5 cut(s)	423, 569, 613, 709, 1063
HspAI	GCGC	1 cut(s)	697
KflI	GGGWCCC	1 cut(s)	252
Kpn2I	TCCGGA	1 cut(s)	721
KroI	GCCGGC	2 cut(s)	1031, 1160
KroNI	GCCGGC	2 cut(s)	1033, 1162
Ksp22I	TGATCA	1 cut(s)	1101
Kzo9I	GATC	7 cut(s)	4, 132, 237, 784, 843, 1081, 1101
LmnI	GCTCC	3 cut(s)	174, 524, 588
Lsp1109I	GCAGC	6 cut(s)	48, 379, 726, 882, 1003, 1031
LweI	GCATC	3 cut(s)	27, 535, 557
MaeII	ACGT	4 cut(s)	36, 117, 960, 1143
MalI	GATC	7 cut(s)	6, 134, 239, 786, 845, 1083, 1103
MbiI	CCGCTC	2 cut(s)	362, 441
MboI	GATC	7 cut(s)	4, 132, 237, 784, 843, 1081, 1101
MflI	RGATCY	2 cut(s)	4, 843
MhlI	GDGCHC	4 cut(s)	228, 593, 1143, 1149
MluCI	AATT	1 cut(s)	415
MlyI	GAGTC	2 cut(s)	779, 929
MmeI	TCCRAC	2 cut(s)	111, 179
Mph1103I	ATGCAT	2 cut(s)	511, 550
MroI	TCCGGA	1 cut(s)	721
MroNI	GCCGGC	2 cut(s)	1031, 1160
MseI	TTAA	3 cut(s)	597, 840, 876
MslI	CAYNNNNRTG	2 cut(s)	116, 992
MspA1I	CMGCKG	3 cut(s)	106, 261, 274
MspCI	CTTAAG	1 cut(s)	875
MspI	CCGG	6 cut(s)	141, 468, 722, 1032, 1119, 1161
MspR9I	CCNGG	5 cut(s)	142, 161, 468, 469, 490
MvaI	CCWGG	2 cut(s)	161, 490
MvnI	CGCG	1 cut(s)	137
MwoI	GCNNNNNNNGC	3 cut(s)	365, 401, 1028
NaeI	GCCGGC	2 cut(s)	1033, 1162
NciI	CCSGG	3 cut(s)	142, 468, 469
NdeII	GATC	7 cut(s)	4, 132, 237, 784, 843, 1081, 1101
NgoMIV	GCCGGC	2 cut(s)	1031, 1160
NlaIII	CATG	5 cut(s)	423, 569, 613, 709, 1063
NlaIV	GGNNCC	5 cut(s)	153, 253, 254, 268, 603
NmeAIII	GCCGAG	1 cut(s)	247
NsiI	ATGCAT	2 cut(s)	511, 550
PagI	TCATGA	1 cut(s)	609
PdiI	GCCGGC	2 cut(s)	1033, 1162
PfeI	GAWTC	1 cut(s)	828
PleI	GAGTC	2 cut(s)	778, 929
PmaCI	CACGTG	2 cut(s)	118, 1144
PmlI	CACGTG	2 cut(s)	118, 1144
PpsI	GAGTC	2 cut(s)	778, 929
Ppu21I	YACGTR	2 cut(s)	118, 1144
PpuMI	RGGWCCY	1 cut(s)	252
PsiI	TTATAA	1 cut(s)	1040
Psp124BI	GAGCTC	1 cut(s)	228
Psp5II	RGGWCCY	1 cut(s)	252
Psp6I	CCWGG	2 cut(s)	159, 488
PspCI	CACGTG	2 cut(s)	118, 1144
PspFI	CCCAGC	2 cut(s)	257, 270
PspGI	CCWGG	2 cut(s)	159, 488
PspN4I	GGNNCC	5 cut(s)	153, 253, 254, 268, 603
PspPI	GGNCC	5 cut(s)	152, 163, 252, 266, 367
PspPPI	RGGWCCY	1 cut(s)	252
PstI	CTGCAG	1 cut(s)	1021
PstNI	CAGNNNCTG	1 cut(s)	1016
PsuI	RGATCY	2 cut(s)	4, 843
PvuII	CAGCTG	1 cut(s)	274
RsaI	GTAC	2 cut(s)	98, 459
RsaNI	GTAC	2 cut(s)	97, 458
RseI	CAYNNNNRTG	2 cut(s)	116, 992
SacI	GAGCTC	1 cut(s)	228
SaqAI	TTAA	3 cut(s)	597, 840, 876
Sau3AI	GATC	7 cut(s)	4, 132, 237, 784, 843, 1081, 1101
Sau96I	GGNCC	5 cut(s)	152, 163, 252, 266, 367
SchI	GAGTC	2 cut(s)	779, 929
ScrFI	CCNGG	5 cut(s)	142, 161, 468, 469, 490
SduI	GDGCHC	4 cut(s)	228, 593, 1143, 1149
SfaNI	GCATC	3 cut(s)	27, 535, 557
SfcI	CTRYAG	1 cut(s)	1017
SfiI	GGCCNNNNNGGCC	1 cut(s)	365
SinI	GGWCC	3 cut(s)	152, 163, 252
SmaI	CCCGGG	1 cut(s)	469
SmiMI	CAYNNNNRTG	2 cut(s)	116, 992
SmlI	CTYRAG	3 cut(s)	182, 317, 875
SmoI	CTYRAG	3 cut(s)	182, 317, 875
Sse9I	AATT	1 cut(s)	415
SsiI	CCGC	9 cut(s)	104, 137, 195, 219, 261, 264, 360, 441, 694
SstI	GAGCTC	1 cut(s)	228
StyD4I	CCNGG	5 cut(s)	140, 159, 466, 467, 488
TaaI	ACNGT	4 cut(s)	595, 650, 1066, 1126
TaiI	ACGT	4 cut(s)	39, 120, 963, 1146
TaqI	TCGA	4 cut(s)	375, 551, 582, 990
TaqII	GACCGA	1 cut(s)	86
TasI	AATT	1 cut(s)	415
TatI	WGTACW	1 cut(s)	96
TauI	GCSGC	7 cut(s)	106, 140, 221, 264, 267, 362, 697
TfiI	GAWTC	1 cut(s)	828
Tru1I	TTAA	3 cut(s)	597, 840, 876
Tru9I	TTAA	3 cut(s)	597, 840, 876
TscAI	CASTG	2 cut(s)	48, 819
TseI	GCWGC	6 cut(s)	61, 392, 739, 895, 1016, 1019
TspDTI	ATGAA	6 cut(s)	408, 549, 598, 976, 1014, 1123
TspGWI	ACGGA	1 cut(s)	223
TspMI	CCCGGG	1 cut(s)	467
TspRI	CASTG	2 cut(s)	48, 819
Vha464I	CTTAAG	1 cut(s)	875
VpaK11BI	GGWCC	3 cut(s)	152, 163, 252
XagI	CCTNNNNNAGG	1 cut(s)	722
XapI	RAATTY	1 cut(s)	415
XcmI	CCANNNNNNNNNTGG	1 cut(s)	581
XmaI	CCCGGG	1 cut(s)	467
ZraI	GACGTC	1 cut(s)	37
Zsp2I	ATGCAT	2 cut(s)	511, 550

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.