MD08G1237600.v1.1

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001027
Physical Location & Seq
Forward (+)
30489013 .. 30491887
2875 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1237600.v1.1.491

Sequence Viewer

Length: 627 bp
ATGCAGCTTCGACGATGGAGTATCGTCATCGTCGCCACAGTCTTTGCAACTGTCATCCAAGCGTTTTCCACGGCAAACTCCCTCCAAGAAGCGGATAAATTCACAAGGTTGCCAGGTCAACCACAAGTCAACTTCCAGCAATATGCAGGATATGTAACTGTGGATGAAAAGCAAGAGAGATCTCTTTTTTACTACTTTGTTGAAGCACAAACTAATCCTGCTTCCAAGCCTCTTGTTCTTTGGCTCAATGGAGGTCCTGGTTGTTCATCTGTTGGAGTTGGAGCTTTCAACGAGAACGGACCTTTTCAACCAAGCAGAGACATTTTACTCAAGAACGATTTTAGTTGGAATAGAGAAGCAAATATGCTGTACTTGGAGTCACCTGCAGGAGTTGGCTTTTCCTACTCAGCAAACAAATCATTTTATGGCTACATGAACGACGATATCACAGCACGAGATAATATCGAGTTCCTTCAGCGCTGGTTCGACAAATTCCCCGAGTATAAAAATAGGGATTTCTTTATCACAGGAGAGAGCTATGCAGGCCACTATGTTCCGCAGCTTGCACAACTCATTATCCAATCAAAACTGAAGTTCAATCTGAAAGCAATAGCAGTAAGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

209

Amino Acids

23.68

Weight (kDa)

7.75

Isoelectric Point (pI)

53.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 38 - 206 2.2e-74 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 391
Acc36I ACCTGC 1 cut(s) 391
AciI CCGC 2 cut(s) 92, 557
AcsI RAATTY 2 cut(s) 98, 491
AcuI CTGAAG 2 cut(s) 458, 611
AfaI GTAC 1 cut(s) 371
AfeI AGCGCT 1 cut(s) 479
AfiI CCNNNNNNNGG 1 cut(s) 91
AgsI TTSAA 4 cut(s) 203, 289, 308, 598
AjnI CCWGG 2 cut(s) 112, 256
AluBI AGCT 4 cut(s) 7, 284, 537, 562
AluI AGCT 4 cut(s) 7, 284, 537, 562
Alw26I GTCTC 1 cut(s) 312
Ama87I CYCGRG 1 cut(s) 497
Aor51HI AGCGCT 1 cut(s) 479
AoxI GGCC 1 cut(s) 544
ApeKI GCWGC 2 cut(s) 4, 559
ApoI RAATTY 2 cut(s) 98, 491
AspLEI GCGC 1 cut(s) 480
AspS9I GGNCC 2 cut(s) 254, 299
AsuHPI GGTGA 1 cut(s) 372
AvaI CYCGRG 1 cut(s) 497
AvaII GGWCC 2 cut(s) 254, 299
BauI CACGAG 1 cut(s) 453
BbvI GCAGC 2 cut(s) 16, 571
BccI CCATC 1 cut(s) 9
BceAI ACGGC 1 cut(s) 87
BciT130I CCWGG 2 cut(s) 114, 258
BcoDI GTCTC 1 cut(s) 312
BfmI CTRYAG 1 cut(s) 384
BfoI RGCGCY 1 cut(s) 481
BfuAI ACCTGC 1 cut(s) 391
BglII AGATCT 1 cut(s) 179
BisI GCNGC 2 cut(s) 5, 560
BlsI GCNGC 2 cut(s) 6, 561
Bme1390I CCNGG 2 cut(s) 114, 258
Bme18I GGWCC 2 cut(s) 254, 299
BmeT110I CYCGRG 1 cut(s) 497
BmgT120I GGNCC 2 cut(s) 254, 299
BmrFI CCNGG 2 cut(s) 114, 258
BpuEI CTTGAG 1 cut(s) 314
BsaJI CCNNGG 1 cut(s) 69
Bsc4I CCNNNNNNNGG 1 cut(s) 91
BseBI CCWGG 2 cut(s) 114, 258
BseDI CCNNGG 1 cut(s) 69
BseGI GGATG 2 cut(s) 54, 169
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMII CTCAG 1 cut(s) 420
BseXI GCAGC 2 cut(s) 16, 571
BshFI GGCC 1 cut(s) 546
BsiHKCI CYCGRG 1 cut(s) 497
BslI CCNNNNNNNGG 1 cut(s) 91
BsmAI GTCTC 1 cut(s) 312
BsnI GGCC 1 cut(s) 546
BsoBI CYCGRG 1 cut(s) 497
Bsp143I GATC 1 cut(s) 179
BspACI CCGC 2 cut(s) 92, 557
BspANI GGCC 1 cut(s) 546
BspCNI CTCAG 1 cut(s) 419
BspMAI CTGCAG 1 cut(s) 388
BspMI ACCTGC 1 cut(s) 391
BssECI CCNNGG 1 cut(s) 69
BssMI GATC 1 cut(s) 179
BssSI CACGAG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 2 cut(s) 114, 258
Bst4CI ACNGT 3 cut(s) 40, 52, 160
BstC8I GCNNGC 2 cut(s) 544, 564
BstDEI CTNAG 1 cut(s) 406
BstDSI CCRYGG 1 cut(s) 69
BstF5I GGATG 2 cut(s) 54, 169
BstH2I RGCGCY 1 cut(s) 481
BstHHI GCGC 1 cut(s) 480
BstKTI GATC 1 cut(s) 182
BstMAI GTCTC 1 cut(s) 312
BstMBI GATC 1 cut(s) 179
BstMWI GCNNNNNNNGC 1 cut(s) 543
BstNI CCWGG 2 cut(s) 114, 258
BstSCI CCNGG 2 cut(s) 112, 256
BstSFI CTRYAG 1 cut(s) 384
BstV1I GCAGC 2 cut(s) 16, 571
BstX2I RGATCY 1 cut(s) 179
BstYI RGATCY 1 cut(s) 179
BsuRI GGCC 1 cut(s) 546
BtgI CCRYGG 1 cut(s) 69
BtsCI GGATG 2 cut(s) 54, 169
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 2 cut(s) 544, 564
CfoI GCGC 1 cut(s) 480
Cfr13I GGNCC 2 cut(s) 254, 299
Csp6I GTAC 1 cut(s) 370
CspCI CAANNNNNGTGG 2 cut(s) 25, 60
CviAII CATG 1 cut(s) 433
CviJI RGCY 9 cut(s) 7, 229, 244, 284, 396, 429, 537, 546, 562
CviKI_1 RGCY 9 cut(s) 7, 229, 244, 284, 396, 429, 537, 546, 562
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 1 cut(s) 406
DpnI GATC 1 cut(s) 181
DpnII GATC 1 cut(s) 179
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 2 cut(s) 254, 299
Eco47III AGCGCT 1 cut(s) 479
Eco57I CTGAAG 2 cut(s) 458, 611
Eco88I CYCGRG 1 cut(s) 497
EcoO109I RGGNCCY 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 112, 256
EcoRV GATATC 1 cut(s) 445
FaeI CATG 1 cut(s) 436
FaiI YATR 8 cut(s) 144, 153, 365, 426, 434, 504, 540, 552
FatI CATG 1 cut(s) 432
Fnu4HI GCNGC 2 cut(s) 5, 560
FokI GGATG 2 cut(s) 41, 176
Fsp4HI GCNGC 2 cut(s) 5, 560
GlaI GCGC 1 cut(s) 479
GluI GCNGC 2 cut(s) 5, 560
HaeII RGCGCY 1 cut(s) 481
HaeIII GGCC 1 cut(s) 546
HhaI GCGC 1 cut(s) 480
Hin1II CATG 1 cut(s) 436
Hin6I GCGC 1 cut(s) 478
HinP1I GCGC 1 cut(s) 478
HincII GTYRAC 2 cut(s) 119, 130
HindII GTYRAC 2 cut(s) 119, 130
HinfI GANTC 1 cut(s) 377
HphI GGTGA 1 cut(s) 372
Hpy166II GTNNAC 2 cut(s) 119, 130
Hpy188I TCNGA 1 cut(s) 603
Hpy188III TCNNGA 1 cut(s) 331
Hpy8I GTNNAC 2 cut(s) 119, 130
Hpy99I CGWCG 3 cut(s) 15, 35, 443
HpyAV CCTTC 1 cut(s) 482
HpyCH4III ACNGT 3 cut(s) 40, 52, 160
HpyCH4V TGCA 6 cut(s) 4, 47, 146, 386, 542, 566
HpyF10VI GCNNNNNNNGC 1 cut(s) 543
HpyF3I CTNAG 1 cut(s) 406
Hsp92II CATG 1 cut(s) 436
HspAI GCGC 1 cut(s) 478
Kzo9I GATC 1 cut(s) 179
LmnI GCTCC 1 cut(s) 281
Lsp1109I GCAGC 2 cut(s) 16, 571
MaeIII GTNAC 2 cut(s) 154, 378
MalI GATC 1 cut(s) 181
MboI GATC 1 cut(s) 179
MflI RGATCY 1 cut(s) 179
MluCI AATT 2 cut(s) 98, 491
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 3 cut(s) 253, 259, 326
MnlI CCTC 3 cut(s) 92, 240, 245
MspR9I CCNGG 2 cut(s) 114, 258
MvaI CCWGG 2 cut(s) 114, 258
MwoI GCNNNNNNNGC 1 cut(s) 543
NdeII GATC 1 cut(s) 179
NlaIII CATG 1 cut(s) 436
NmuCI GTSAC 1 cut(s) 378
PaqCI CACCTGC 1 cut(s) 391
PkrI GCNGC 2 cut(s) 6, 561
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
PpuMI RGGWCCY 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 254
Psp6I CCWGG 2 cut(s) 112, 256
PspGI CCWGG 2 cut(s) 112, 256
PspPI GGNCC 2 cut(s) 254, 299
PspPPI RGGWCCY 1 cut(s) 254
PstI CTGCAG 1 cut(s) 388
PsuI RGATCY 1 cut(s) 179
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
SatI GCNGC 2 cut(s) 5, 560
Sau3AI GATC 1 cut(s) 179
Sau96I GGNCC 2 cut(s) 254, 299
SbfI CCTGCAGG 1 cut(s) 388
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 2 cut(s) 114, 258
SdaI CCTGCAGG 1 cut(s) 388
SetI ASST 9 cut(s) 9, 110, 118, 256, 286, 304, 385, 539, 564
SfcI CTRYAG 1 cut(s) 384
SinI GGWCC 2 cut(s) 254, 299
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse8387I CCTGCAGG 1 cut(s) 388
Sse9I AATT 2 cut(s) 98, 491
SsiI CCGC 2 cut(s) 92, 557
StyD4I CCNGG 2 cut(s) 112, 256
TaaI ACNGT 3 cut(s) 40, 52, 160
TaqI TCGA 3 cut(s) 10, 465, 486
TasI AATT 2 cut(s) 98, 491
TatI WGTACW 1 cut(s) 369
TseFI GTSAC 1 cut(s) 378
TseI GCWGC 2 cut(s) 4, 559
Tsp45I GTSAC 1 cut(s) 378
TspDTI ATGAA 3 cut(s) 180, 255, 449
TspGWI ACGGA 1 cut(s) 312
VpaK11BI GGWCC 2 cut(s) 254, 299
XapI RAATTY 2 cut(s) 98, 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.